Browse publications by year: 2015

  1. Chan KG, Kher HL, Chang CY, Yin WF, Tan KH
    Genome Announc, 2015;3(2).
    PMID: 25792063 DOI: 10.1128/genomeA.00145-15
    Dickeya chrysanthemi is well known as a plant pathogen that caused major blackleg in the European potato industry in the 1990s. D. chrysanthemi strain L11 was discovered in a recreational lake in Malaysia. Here, we present its draft genome sequence.
    MeSH terms: Chromosome Mapping; Malaysia; Solanum tuberosum; Pectobacterium chrysanthemi; Plant Tubers; Lakes
  2. Nordin N, Majid NA, Hashim NM, Rahman MA, Hassan Z, Ali HM
    Drug Des Devel Ther, 2015;9:1437-48.
    PMID: 25792804 DOI: 10.2147/DDDT.S77727
    Enicosanthellum pulchrum is a tropical plant from Malaysia and belongs to the Annonaceae family. This plant is rich in isoquinoline alkaloids. In the present study, liriodenine, an isoquinoline alkaloid, was examined as a potential anticancer agent, particularly in ovarian cancer. Liriodenine was isolated by preparative high-performance liquid chromatography. Cell viability was performed to determine the cytotoxicity, whilst the detection of morphological changes was carried out by acridine orange/propidium iodide assay. Initial and late apoptosis was examined by Annexin V-fluorescein isothiocyanate and DNA laddering assays, respectively. The involvement of pathways was detected via caspase-3, caspase-8, and caspase-9 analyses. Confirmation of pathways was further performed in mitochondria using a cytotoxicity 3 assay. Apoptosis was confirmed at the protein level, including Bax, Bcl-2, and survivin, while interruption of the cell cycle was used for final validation of apoptosis. The result showed that liriodenine inhibits proliferation of CAOV-3 cells at 37.3 μM after 24 hours of exposure. Changes in cell morphology were detected by the presence of cell membrane blebbing, chromatin condensation, and formation of apoptotic bodies. Early apoptosis was observed by Annexin V-fluorescein isothiocyanate bound to the cell membrane as early as 24 hours. Liriodenine activated the intrinsic pathway by induction of caspase-3 and caspase-9. Involvement of the intrinsic pathway in the mitochondria could be seen, with a significant increase in mitochondrial permeability and cytochrome c release, whereas the mitochondrial membrane potential was decreased. DNA fragmentation occurred at 72 hours upon exposure to liriodenine. The presence of DNA fragmentation indicates the CAOV-3 cells undergo late apoptosis or final stage of apoptosis. Confirmation of apoptosis at the protein level showed overexpression of Bax and suppression of Bcl-2 and survivin. Liriodenine inhibits progression of the CAOV-3 cell cycle in S phase. These findings indicate that liriodenine could be considered as a promising anticancer agent.
  3. Loh SY, Musa AN
    PMID: 25792854 DOI: 10.2147/BCTT.S47012
    CONTEXT: Breast cancer is the most prevalent cancer amongst women but it has the highest survival rates amongst all cancer. Rehabilitation therapy of post-treatment effects from cancer and its treatment is needed to improve functioning and quality of life. This review investigated the range of methods for improving physical, psychosocial, occupational, and social wellbeing in women with breast cancer after receiving breast cancer surgery.
    METHOD: A search for articles published in English between the years 2009 and 2014 was carried out using The Cochrane Database of Systematic Reviews, the Database of Abstracts of Reviews of Effects, PubMed, and ScienceDirect. Search terms included: 'breast cancer', 'breast carcinoma', 'surgery', 'mastectomy', 'lumpectomy', 'breast conservation', 'axillary lymph node dissection', 'rehabilitation', 'therapy', 'physiotherapy', 'occupational therapy', 'psychological', 'psychosocial', 'psychotherapy', 'exercise', 'physical activity', 'cognitive', 'occupational', 'alternative', 'complementary', and 'systematic review'.
    STUDY SELECTION: Systematic reviews on the effectiveness of rehabilitation methods in improving post-operative physical, and psychological outcomes for breast cancer were selected. Sixteen articles met all the eligibility criteria and were included in the review.
    DATA EXTRACTION: Included review year, study aim, total number of participants included, and results.
    DATA SYNTHESIS: Evidence for exercise rehabilitation is predominantly in the improvement of shoulder mobility and limb strength. Inconclusive results exist for a range of rehabilitation methods (physical, psycho-education, nutritional, alternative-complementary methods) for addressing the domains of psychosocial, cognitive, and occupational outcomes.
    CONCLUSION: There is good evidence for narrowly-focused exercise rehabilitation in improving physical outcome particularly for shoulder mobility and lymphedema. There were inconclusive results for methods to improve psychosocial, cognitive, and occupational outcomes. There were no reviews on broader performance areas and lifestyle factors to enable effective living after treatment. The review suggests that comprehensiveness and effectiveness of post-operative breast cancer rehabilitation should consider patients' self-management approaches towards lifestyle redesign, and incorporate health promotion aspects, in light of the fact that breast cancer is now taking the form of a chronic illness with longer survivorship years.
    KEYWORDS: breast cancer surgery; lifestyle redesign; quality of life; rehabilitation methods; self-management; symptom-management
    MeSH terms: Breast Neoplasms; Chronic Disease; Cognition; Exercise Therapy; Female; Health Promotion; Humans; Life Style; Lymph Node Excision; Lymphedema; Mastectomy; Occupational Therapy; Psychotherapy; Quality of Life; Shoulder; Mastectomy, Segmental; Exercise; Survival Rate; Physical Therapy Modalities; PubMed
  4. Ravindran S, Jambek AB, Muthusamy H, Neoh SC
    Comput Math Methods Med, 2015;2015:283532.
    PMID: 25793009 DOI: 10.1155/2015/283532
    A novel clinical decision support system is proposed in this paper for evaluating the fetal well-being from the cardiotocogram (CTG) dataset through an Improved Adaptive Genetic Algorithm (IAGA) and Extreme Learning Machine (ELM). IAGA employs a new scaling technique (called sigma scaling) to avoid premature convergence and applies adaptive crossover and mutation techniques with masking concepts to enhance population diversity. Also, this search algorithm utilizes three different fitness functions (two single objective fitness functions and multi-objective fitness function) to assess its performance. The classification results unfold that promising classification accuracy of 94% is obtained with an optimal feature subset using IAGA. Also, the classification results are compared with those of other Feature Reduction techniques to substantiate its exhaustive search towards the global optimum. Besides, five other benchmark datasets are used to gauge the strength of the proposed IAGA algorithm.
    MeSH terms: Machine Learning; Algorithms; Female; Fetal Monitoring/methods*; Humans; Mutation; Pregnancy; Software; Stochastic Processes; Cardiotocography/methods; Reproducibility of Results; Area Under Curve; Decision Support Systems, Clinical*; Support Vector Machine
  5. Alomari YM, Sheikh Abdullah SN, MdZin RR, Omar K
    Comput Math Methods Med, 2015;2015:673658.
    PMID: 25793010 DOI: 10.1155/2015/673658
    Analysis of whole-slide tissue for digital pathology images has been clinically approved to provide a second opinion to pathologists. Localization of focus points from Ki-67-stained histopathology whole-slide tissue microscopic images is considered the first step in the process of proliferation rate estimation. Pathologists use eye pooling or eagle-view techniques to localize the highly stained cell-concentrated regions from the whole slide under microscope, which is called focus-point regions. This procedure leads to a high variety of interpersonal observations and time consuming, tedious work and causes inaccurate findings. The localization of focus-point regions can be addressed as a clustering problem. This paper aims to automate the localization of focus-point regions from whole-slide images using the random patch probabilistic density method. Unlike other clustering methods, random patch probabilistic density method can adaptively localize focus-point regions without predetermining the number of clusters. The proposed method was compared with the k-means and fuzzy c-means clustering methods. Our proposed method achieves a good performance, when the results were evaluated by three expert pathologists. The proposed method achieves an average false-positive rate of 0.84% for the focus-point region localization error. Moreover, regarding RPPD used to localize tissue from whole-slide images, 228 whole-slide images have been tested; 97.3% localization accuracy was achieved.
    MeSH terms: Algorithms; Brain/metabolism; Brain Neoplasms/metabolism*; Diagnosis, Computer-Assisted/methods*; Humans; Microscopy; Pattern Recognition, Automated; Probability; Software; Reproducibility of Results; Gene Expression Regulation, Neoplastic*; Cluster Analysis; Computational Biology; Ki-67 Antigen/metabolism*; Cell Proliferation
  6. Seman NA, Mohamud WN, Östenson CG, Brismar K, Gu HF
    Clin Epigenetics, 2015;7:30.
    PMID: 25793017 DOI: 10.1186/s13148-015-0049-5
    Recent studies have demonstrated that DNA polymorphisms in the solute carrier family 30 member 8 (SLC30A8) gene confer the risk susceptibility to type 2 diabetes (T2D). The present study aimed to analyze DNA methylation levels of this gene in T2D and diabetic nephropathy (DN).
  7. Sze TL, Abdul Aziz YF, Abu Bakar N, Mohd Sani F, Oemar H
    Iran J Radiol, 2015 Jan;12(1):e6878.
    PMID: 25793089 DOI: 10.5812/iranjradiol.6878
    Coronary artery fistula (CAF) is a rare anomaly of the coronary artery. Patients with this condition are usually asymptomatic. However, cardiac failure may occur later in life due to progressive enlargement of the fistula. Diagnosis is traditionally made by echocardiogram and conventional angiogram. However with the advantage of new technologies such as computed tomography (CT) coronary angiography, the course and communications of these fistulae can be delineated non-invasively and with greater accuracy. We report a case of a left circumflex artery fistula to the coronary sinus which was suspected on echocardiogram and the diagnosis was clinched on ECG-gated CT.
    MeSH terms: Coronary Artery Disease; Echocardiography; Electrocardiography; Fistula; Heart Failure; Humans; Tomography, X-Ray Computed; Coronary Angiography; Coronary Sinus
  8. Karthivashan G, Arulselvan P, Alimon AR, Safinar Ismail I, Fakurazi S
    Biomed Res Int, 2015;2015:970398.
    PMID: 25793214 DOI: 10.1155/2015/970398
    The influence of Moringa oleifera (MO) leaf extract as a dietary supplement on the growth performance and antioxidant parameters was evaluated on broiler meat and the compounds responsible for the corresponding antioxidant activity were identified. 0.5%, 1.0%, and 1.5% w/v of MO leaf aqueous extracts (MOLE) were prepared, and nutritional feed supplemented with 0%, 0.5%, 1.0%, and 1.5% w/w of MO leaf meal (MOLM) extracts were also prepared and analysed for their in vitro antioxidant potential. Furthermore, the treated broiler groups (control (T1) and treatment (T2, T3, and T4)) were evaluated for performance, meat quality, and antioxidant status. The results of this study revealed that, among the broilers fed MOLM, the broilers fed 0.5% w/w MOLM (T2) exhibited enhanced meat quality and antioxidant status (P < 0.05). However, the antioxidant activity of the MOLE is greater than that of the MOLM. The LC-MS/MS analysis of MOLM showed high expression of isoflavones and fatty acids from soy and corn source, which antagonistically inhibit the expression of the flavonoids/phenols in the MO leaves thereby masking its antioxidant effects. Thus, altering the soy and corn gradients in conventional nutrition feed with 0.5% w/w MO leaves supplement would provide an efficient and cost-effective feed supplement.
    MeSH terms: Animals; Animal Feed; Antioxidants/pharmacology*; Antioxidants/chemistry; Chickens/growth & development*; Fatty Acids/pharmacology; Fatty Acids/chemistry; Isoflavones/pharmacology; Isoflavones/chemistry; Meat; Nutritional Status/physiology; Plant Extracts/pharmacology*; Plant Extracts/chemistry; Plant Leaves/chemistry; Dietary Supplements; Moringa oleifera/chemistry*
  9. Omar L, Ahmed OH, Ab Majid NM
    ScientificWorldJournal, 2015;2015:574201.
    PMID: 25793220 DOI: 10.1155/2015/574201
    Improper use of urea may cause environmental pollution through NH3 volatilization and NO3 (-) leaching from urea. Clinoptilolite zeolite and compost could be used to control N loss from urea by controlling NH4 (+) and NO3 (-) release from urea. Soil incubation and leaching experiments were conducted to determine the effects of clinoptilolite zeolite and compost on controlling NH4 (+) and NO3 (-) losses from urea. Bekenu Series soil (Typic Paleudults) was incubated for 30, 60, and 90 days. A soil leaching experiment was conducted for 30 days. Urea amended with clinoptilolite zeolite and compost significantly reduced NH4 (+) and NO3 (-) release from urea (soil incubation study) compared with urea alone, thus reducing leaching of these ions. Ammonium and NO3 (-) leaching losses during the 30 days of the leaching experiment were highest in urea alone compared with urea with clinoptilolite zeolite and compost treatments. At 30 days of the leaching experiment, NH4 (+) retention in soil with urea amended with clinoptilolite zeolite and compost was better than that with urea alone. These observations were because of the high pH, CEC, and other chemical properties of clinoptilolite zeolite and compost. Urea can be amended with clinoptilolite zeolite and compost to improve NH4 (+) and NO3 (-) release from urea.
    MeSH terms: Agriculture; Nitrates/analysis*; Soil/chemistry; Soil Pollutants/analysis; Urea/chemistry*; Waste Products/analysis; Zeolites*; Crops, Agricultural/chemistry; Ammonium Compounds/analysis*
  10. Ramanathan S, Parthasarathy S, Murugaiyah V, Magosso E, Tan SC, Mansor SM
    Molecules, 2015 Mar 18;20(3):4915-27.
    PMID: 25793541 DOI: 10.3390/molecules20034915
    Varied pharmacological responses have been reported for mitragynine in the literature, but no supportive scientific explanations have been given for this. These studies have been undertaken without a sufficient understanding of the physicochemical properties of mitragynine. In this work a UV spectrophotometer approach and HPLC-UV method were employed to ascertain the physicochemical properties of mitragynine. The pKa of mitragynine measured by conventional UV (8.11 ± 0.11) was in agreement with the microplate reader determination (8.08 ± 0.04). Mitragynine is a lipophilic alkaloid, as indicated by a logP value of 1.73. Mitragynine had poor solubility in water and basic media, and conversely in acidic environments, but it is acid labile. In an in vitro dissolution the total drug release was higher for the simulated gastric fluid but was prolonged and incomplete for the simulated intestinal fluid. The hydrophobicity, poor water solubility, high variability of drug release in simulated biological fluids and acid degradable characteristics of mitragynine probably explain the large variability of its pharmacological responses reported in the literature. The determined physicochemical properties of mitragynine will provide a basis for developing a suitable formulation to further improve its solubility, stability and oral absorption for better assessment of this compound in preclinical studies.
    MeSH terms: Chromatography, High Pressure Liquid/methods; Drug Evaluation, Preclinical; Solubility; Spectrophotometry, Ultraviolet/methods; Plant Leaves/chemistry; Mitragyna/chemistry*; Secologanin Tryptamine Alkaloids/isolation & purification*; Secologanin Tryptamine Alkaloids/chemistry*
  11. Ain MK, Rosdinom R, Raynuha M
    Int Psychogeriatr, 2015 Sep;27(9):1573-5.
    PMID: 25794136 DOI: 10.1017/S1041610215000332
    We report a Malay man, with underlying chronic medical illnesses, presenting with positive symptoms of schizophrenia, including Capgras syndrome (CS) at the age of 73. Physical examination and blood investigations were normal and brain CT scan showed age-related cerebral atrophy. Neuropsychological assessment showed probable right hemisphere lesions but relatively intact memory and intellectual functions. Several neuroleptics including depot injections were tried but ineffective. Positive symptoms including CS eventually improved with clozapine before his death from myocardial infarction. This case report highlights the uncommon occurrence of CS in treatment resistant schizophrenia (TRS) of very late onset and its treatment challenges.
    MeSH terms: Late Onset Disorders; Aged; Autopsy; Capgras Syndrome/diagnosis*; Capgras Syndrome/drug therapy*; Clozapine/therapeutic use*; Delusions; Humans; Male; Neuropsychological Tests; Psychiatric Status Rating Scales; Schizophrenia/diagnosis*; Schizophrenia/drug therapy*; Schizophrenic Psychology; Antipsychotic Agents/therapeutic use*; Comorbidity
  12. Al-Darraji HA, Tan C, Kamarulzaman A, Altice FL
    Occup Environ Med, 2015 Jun;72(6):442-7.
    PMID: 25794506 DOI: 10.1136/oemed-2014-102695
    OBJECTIVES: Although prison employees share the same tuberculosis (TB) risk environment with prisoners, the magnitude of TB problems among prison employees is unknown in most resource-limited prisons. This survey was conducted to investigate the prevalence and correlates of tuberculin skin test (TST) positivity among employees in Malaysia's largest prison.

    METHODS: Consented, full-time prison employees were interviewed using a structured questionnaire that included sociodemographic data, history of working in the correctional system and TB-related risk. TST was placed intradermally and read after 48-72 h. Induration size of ≥10 mm was considered positive. Logistic regression analyses were conducted to explore associations with TST positivity.

    RESULTS: Of the 445 recruited prison employees, 420 (94.4%) had complete data. Most were young (median=30.0 years) men (88.8%) who had only worked at this prison (76.4%) for a median total employment period of 60 months (IQR 34.5-132.0). The majority were correctional officers, while civilian employees represented only 7.6% of the sample. Only 26 (6.2%) reported having ever been screened for TB since employment. Prevalence of TST positivity was 81% and was independently associated with longer (≥12 months) prison employment (AOR 4.9; 95% CI 1.5 to 15.9) and current tobacco smoking (AOR=1.9, 95% CI 1.2 to 3.2).

    CONCLUSIONS: Latent TB prevalence was high in this sample, approximating that of prisoners in this setting, perhaps suggesting within prison TB transmission in this facility. Formal TB control programmes for personnel and prisoners alike are urgently needed within the Malaysian correctional system.

    MeSH terms: Adult; Cross-Sectional Studies; Female; Humans; Malaysia/epidemiology; Male; Middle Aged; Occupational Diseases/etiology; Occupational Diseases/epidemiology*; Prisons/statistics & numerical data*; Risk Factors; Prevalence; Logistic Models; Young Adult; Latent Tuberculosis/etiology; Latent Tuberculosis/epidemiology*
  13. Wo MC, Lim KS, Choo WY, Tan CT
    Epilepsy Behav, 2015 Apr;45:21-30.
    PMID: 25794681 DOI: 10.1016/j.yebeh.2015.02.016
    This study aimed to explore positive and negative factors affecting the employability in patients with uncontrolled seizures.
    MeSH terms: Adult; Automobile Driving/psychology*; Employment/psychology*; Female; Humans; Male; Marriage; Middle Aged; Seizures/psychology*; Self Concept*; Social Support; Unemployment/psychology*; Social Stigma
  14. He Z, Tan JS, Lai OM, Ariff AB
    Food Chem, 2015 Aug 15;181:19-24.
    PMID: 25794715 DOI: 10.1016/j.foodchem.2014.11.166
    In this study, the methods for extraction and purification of miraculin from Synsepalum dulcificum were investigated. For extraction, the effect of different extraction buffers (phosphate buffer saline, Tris-HCl and NaCl) on the extraction efficiency of total protein was evaluated. Immobilized metal ion affinity chromatography (IMAC) with nickel-NTA was used for the purification of the extracted protein, where the influence of binding buffer pH, crude extract pH and imidazole concentration in elution buffer upon the purification performance was explored. The total amount of protein extracted from miracle fruit was found to be 4 times higher using 0.5M NaCl as compared to Tris-HCl and phosphate buffer saline. On the other hand, the use of Tris-HCl as binding buffer gave higher purification performance than sodium phosphate and citrate-phosphate buffers in IMAC system. The optimum purification condition of miraculin using IMAC was achieved with crude extract at pH 7, Tris-HCl binding buffer at pH 7 and the use of 300 mM imidazole as elution buffer, which gave the overall yield of 80.3% and purity of 97.5%. IMAC with nickel-NTA was successfully used as a single step process for the purification of miraculin from crude extract of S. dulcificum.
    MeSH terms: Chromatography, Affinity/methods; Glycoproteins/chemistry*; Imidazoles; Metals/chemistry*; Synsepalum/chemistry*
  15. Mushtaq F, Abdullah TA, Mat R, Ani FN
    Bioresour Technol, 2015 Aug;190:442-50.
    PMID: 25794811 DOI: 10.1016/j.biortech.2015.02.055
    In this study, solid oil palm shell (OPS) waste biomass was subjected to microwave pyrolysis conditions with uniformly distributed coconut activated carbon (CAC) microwave absorber. The effects of CAC loading (wt%), microwave power (W) and N2 flow rate (LPM) were investigated on heating profile, bio-oil yield and its composition. Response surface methodology based on central composite design was used to study the significance of process parameters on bio-oil yield. The coefficient of determination (R(2)) for the bio-oil yield is 0.89017 indicating 89.017% of data variability is accounted to the model. The largest effect on bio-oil yield is from linear and quadratic terms of N2 flow rate. The phenol content in bio-oil is 32.24-58.09% GC-MS area. The bio-oil also contain 1,1-dimethyl hydrazine of 10.54-21.20% GC-MS area. The presence of phenol and 1,1-dimethyl hydrazine implies that the microwave pyrolysis of OPS with carbon absorber has the potential to produce valuable fuel products.
    MeSH terms: Agriculture/methods; Biodegradation, Environmental; Computer Simulation; Heating/methods*; Industrial Waste/prevention & control; Microwaves*; Models, Chemical; Plant Extracts/radiation effects; Plant Extracts/chemistry*; Radiation Dosage; Refuse Disposal/methods*; Arecaceae/radiation effects; Arecaceae/chemistry*; Biofuels/analysis; Biofuels/radiation effects*; Absorption, Radiation
  16. Yew KL, Kang Z
    Int J Cardiol, 2015 Apr 15;185:150-2.
    PMID: 25795207 DOI: 10.1016/j.ijcard.2015.03.127
    MeSH terms: Aged; Coronary Vessel Anomalies/complications; Coronary Vessel Anomalies/radiography; Coronary Vessel Anomalies/surgery*; Coronary Vessels/radiography; Coronary Vessels/surgery*; Humans; Male; Myocardial Infarction/etiology; Myocardial Infarction/radiography; Myocardial Infarction/surgery*; Prosthesis Design; Stents*; Coronary Angiography; Radial Artery; Percutaneous Coronary Intervention/methods*
  17. Yew KL
    Int J Cardiol, 2015 Apr 15;185:155-6.
    PMID: 25795209 DOI: 10.1016/j.ijcard.2015.03.003
    MeSH terms: Coronary Vessel Anomalies/complications; Coronary Vessel Anomalies/radiography; Coronary Vessel Anomalies/surgery*; Coronary Vessels/radiography; Coronary Vessels/surgery*; Humans; Immunosuppressive Agents/pharmacology; Male; Middle Aged; Prosthesis Design; Coronary Angiography; Sirolimus/pharmacology*; Acute Coronary Syndrome/etiology; Acute Coronary Syndrome/radiography; Acute Coronary Syndrome/surgery*; Drug-Eluting Stents*; Percutaneous Coronary Intervention/methods*
  18. Foo JB, Saiful Yazan L, Tor YS, Wibowo A, Ismail N, How CW, et al.
    J Ethnopharmacol, 2015 May 26;166:270-8.
    PMID: 25797115 DOI: 10.1016/j.jep.2015.03.039
    Dillenia suffruticosa (Family: Dilleniaceae) or commonly known as "Simpoh air" in Malaysia, is traditionally used for treatment of cancerous growth including breast cancer.
    MeSH terms: Humans; Mitochondria/drug effects*; Mitochondria/metabolism; Plant Extracts/pharmacology; Plant Extracts/chemistry; Protein Processing, Post-Translational/drug effects; RNA, Messenger/metabolism; Triterpenes/pharmacology*; Signal Transduction/drug effects; Tumor Suppressor Protein p53/metabolism*; G0 Phase/drug effects; Apoptosis/drug effects*; Plant Roots/chemistry; Proto-Oncogene Proteins c-bcl-2/metabolism; Dilleniaceae/chemistry*; Cell Line, Tumor; Cyclin-Dependent Kinase Inhibitor p21/metabolism*; Apoptosis Regulatory Proteins/metabolism; bcl-2-Associated X Protein/drug effects; G1 Phase Cell Cycle Checkpoints/drug effects*; MCF-7 Cells
  19. Fiala I, Hlavničková M, Kodádková A, Freeman MA, Bartošová-Sojková P, Atkinson SD
    Mol Phylogenet Evol, 2015 May;86:75-89.
    PMID: 25797924 DOI: 10.1016/j.ympev.2015.03.004
    In order to clarify the phylogenetic relationships among the main marine myxosporean clades including newly established Ceratonova clade and scrutinizing their evolutionary origins, we performed large-scale phylogenetic analysis of all myxosporean species from the marine myxosporean lineage based on three gene analyses and statistical topology tests. Furthermore, we obtained new molecular data for Ceratonova shasta, C. gasterostea, eight Ceratomyxa species and one Myxodavisia species. We described five new species: Ceratomyxa ayami n. sp., C. leatherjacketi n. sp., C. synaphobranchi n. sp., C. verudaensis n. sp. and Myxodavisia bulani n. sp.; two of these formed a new, basal Ceratomyxa subclade. We identified that the Ceratomyxa clade is basal to all other marine myxosporean lineages, and Kudoa with Enteromyxum are the most recently branching clades. Topologies were least stable at the nodes connecting the marine urinary clade, the marine gall bladder clade and the Ceratonova clade. Bayesian inference analysis of SSU rDNA and the statistical tree topology tests suggested that Ceratonova is closely related to the Enteromyxum and Kudoa clades, which represent a large group of histozoic species. A close relationship between Ceratomyxa and Ceratonova was not supported, despite their similar myxospore morphologies. Overall, the site of sporulation in the vertebrate host is a more accurate predictor of phylogenetic relationships than the morphology of the myxospore.
    MeSH terms: Animals; Bayes Theorem; DNA, Ribosomal/genetics; Biological Evolution*; Models, Genetic; Phylogeny*; Likelihood Functions; Sequence Analysis, DNA; Myxozoa/classification*
  20. Basri R, Zueter AR, Mohamed Z, Alam MK, Norsa'adah B, Hasan SA, et al.
    Nagoya J Med Sci, 2015 Feb;77(1-2):59-68.
    PMID: 25797971
    To describe the clinical characteristics and the risk factors associated with mortality in patients with meningitis. This is a retrospective review of patients diagnosed to have meningitis with positive culture of the cerebrospinal fluid (CSF) specimen. All cases aged 19 > years who were admitted to Hospital USM between January 2004 and December 2011 were included in the study. The CSF results database were obtained from the Department of Medical Microbiology and Parasitology, Hospital USM, Kelantan. A checklist was used to record the clinical characteristics. A total of 125 cases met the inclusion criteria. The age of patients ranged between newborn and 19 years old (Mean±SD, 74.5±80.6 months). The majority of them were males (65.6%). Fever was the most common presentation (73.6%) followed by poor oral intake (48.0%), seizure (36.0%) and headache (24.8%). The mortality rate was 31.2%. Coagulase negative staphylococcus was the most frequent pathogens isolated (21.6%), followed by Acinetobacter spp. (17.6%), Staphylococcus aureus (13.6%), Streptococcus spp. (11.2%) and Klebsiella pneumoniae (6.4%). There were significant association of in-hospital death with age (p=0.020) and conscious level (p=0.001). Infectious meningitis is a big health concern, especially among children. We found that coagulase negative staphylococcus, Acinetobacter species, S. aureus, Streptococcus spp and K. pneumoniae were prevalent in our hospital. These microorganisms were hospital associated pathogens. The 31% mortality linked to hospital acquired meningitis specifies the need for focused physician attention especially among younger aged patients.
    MeSH terms: Acinetobacter; Adult; Child; Coagulase; Headache; Humans; Infant, Newborn; Klebsiella pneumoniae; Male; Meningitis; Retrospective Studies; Risk Factors; Seizures; Staphylococcal Infections; Staphylococcus; Staphylococcus aureus; Streptococcus; Prevalence; Hospital Mortality; Young Adult; Checklist
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