Browse publications by year: 2017

  1. Quar TK, Soli SD, Chan YF, Ishak WS, Abdul Wahat NH
    Int J Audiol, 2017 02;56(2):92-98.
    PMID: 27686009 DOI: 10.1080/14992027.2016.1210828
    OBJECTIVE: This study was conducted to evaluate the speech perception of Malaysian Chinese adults using the Taiwanese Mandarin HINT (MHINT-T) and the Malay HINT (MyHINT).

    DESIGN: The MHINT-T and the MyHINT were presented in quiet and noise (front, right and left) conditions under headphones. Results for the two tests were compared with each other and with the norms for each test.

    STUDY SAMPLE: Malaysian Chinese native speakers of Mandarin (N = 58), 18-31 years of age with normal hearing.

    RESULTS: On average, subjects demonstrated poorer speech perception ability than the normative samples for these tests. Repeated measures ANOVA showed that speech reception thresholds (SRTs) were slightly poorer on the MHINT-T than on the MyHINT for all test conditions. However, normalized SRTs were poorer by 0.6 standard deviations for MyHINT as compared with MHINT-T.

    CONCLUSIONS: MyHINT and MHINT-T can be used as norm-referenced speech perception measures for Mandarin-speaking Chinese in Malaysia.

    MeSH terms: Acoustic Stimulation/methods*; Adolescent; Adult; Audiometry, Speech/methods*; Auditory Threshold; Female; Hearing; Humans; Male; Noise/adverse effects*; Perceptual Masking*; Predictive Value of Tests; Speech Intelligibility; Speech Perception*; Multilingualism*; Young Adult
  2. Abduljabbar T, Javed F, Shah A, Samer MS, Vohra F, Akram Z
    Lasers Med Sci, 2017 Feb;32(2):449-459.
    PMID: 27686888 DOI: 10.1007/s10103-016-2086-5
    The aim of the study was to assess the efficacy of adjunctive use of laser therapy (LT) alone or antimicrobial photodynamic therapy (aPDT) to improve clinical periodontal and HbA1c levels in patients with both chronic periodontitis (CP) and type 2 diabetes mellitus (T2DM). Electronic search of the MEDLINE, PubMed, EMBASE, Science Direct, and SCOPUS databases were combined with hand searching of articles published from 1975 up to and including March 2016 using relevant MeSH terms. Six studies were selected for this review. In these six studies, laser treatment was applied, after scaling and root planing (SRP), in two ways: Three studies used laser alone and three studies used laser with photosensitizer. All the six included studies reporting clinical periodontal and glycemic parameters showed that LT and aPDT were effective in the treatment of CP in T2DM subjects at follow-up. Two studies showed significantly better periodontal outcomes for LT as an adjunct to SRP as compared to SRP alone, whereas four studies showed comparable periodontal outcomes among adjunctive LT or aPDT with SRP. Two studies showed significant reduction of HbA1c levels in LT and aPDT as compared to SRP, whereas three studies showed comparable percentage levels at follow-up. It remains debatable whether LT or aPDT as adjunct to SRP is more effective as compared to SRP alone in the improvement of clinical periodontal and glycemic control in patients with both CP and T2DM, given that the scientific evidence is weak.
    MeSH terms: Adult; Aged; Diabetes Mellitus, Type 2/therapy*; Female; Humans; Lasers*; Male; Middle Aged; Periodontium/radiation effects; Dental Scaling/methods*; Root Planing/methods*; Treatment Outcome; Photosensitizing Agents/therapeutic use
  3. Mohamad N, Buang F, Mat Lazim A, Ahmad N, Martin C, Mohd Amin MCI
    J Biomed Mater Res B Appl Biomater, 2017 Nov;105(8):2553-2564.
    PMID: 27690276 DOI: 10.1002/jbm.b.33776
    The use of bacterial cellulose (BC)-based hydrogel has been gaining attention owing to its biocompatibility and biodegradability. This study was designed to investigate the effect of radiation doses and acrylic acid (AA) composition on in vitro and in vivo biocompatibility of BC/AA as wound dressing materials. Physical properties of the hydrogel, that is, thickness, adhesiveness, rate of water vapor transmission, and swelling were measured. Moreover, the effect of these parameters on skin irritation and sensitization, blood compatibility, and cytotoxicity was studied. Increased AA content and irradiation doses increased the thickness, crosslinking density, and improved the mechanical properties of the hydrogel, but reduced its adhesiveness. The swelling capacity of the hydrogel increased significantly with a decrease in the AA composition in simulated wound fluid. The water vapor permeability of polymeric hydrogels was in the range of 2035-2666 [g/(m-2  day-1 )]. Dermal irritation and sensitization test demonstrated that the hydrogel was nonirritant and nonallergic. The BC/AA hydrogel was found to be nontoxic to primary human dermal fibroblast skin cells with viability >88% and was found to be biocompatible with blood with a low hemolytic index (0.80-1.30%). Collectively, these results indicate that these hydrogels have the potential to be used as wound dressings. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2553-2564, 2017.
    MeSH terms: Animals; Bacteria/chemistry*; Bandages*; Beta Particles; Dose-Response Relationship, Radiation; Humans; Materials Testing*; Rabbits; Rats
  4. Rehman FU, Shah KU, Shah SU, Khan IU, Khan GM, Khan A
    Expert Opin Drug Deliv, 2017 Nov;14(11):1325-1340.
    PMID: 27485144 DOI: 10.1080/17425247.2016.1218462
    INTRODUCTION: Lipid-based drug delivery systems (LBDDS) are the most promising technique to formulate the poorly water soluble drugs. Nanotechnology strongly influences the therapeutic performance of hydrophobic drugs and has become an essential approach in drug delivery research. Self-nanoemulsifying drug delivery systems (SNEDDS) are a vital strategy that combines benefits of LBDDS and nanotechnology. SNEDDS are now preferred to improve the formulation of drugs with poor aqueous solubility. Areas covered: The review in its first part shortly describes the LBDDS, nanoemulsions and clarifies the ambiguity between nanoemulsions and microemulsions. In the second part, the review discusses SNEDDS and elaborates on the current developments and modifications in this area without discussing their associated preparation techniques and excipient properties. Expert opinion: SNEDDS have exhibit the potential to increase the bioavailability of poorly water soluble drugs. The stability of SNEDDS is further increased by solidification. Controlled release and supersaturation can be achieved, and are associated with increased patient compliance and improved drug loads, respectively. Presence of biodegradable ingredients and ease of large-scale manufacturing combined with a lot of 'drug-targeting opportunities' give SNEDDS a clear distinction and prominence over other solubility enhancement techniques.
    MeSH terms: Pharmaceutical Preparations/administration & dosage; Pharmaceutical Preparations/chemistry; Emulsions/administration & dosage*; Emulsions/chemistry; Drug Delivery Systems*; Nanotechnology
  5. Breadmore MC, Wuethrich A, Li F, Phung SC, Kalsoom U, Cabot JM, et al.
    Electrophoresis, 2017 01;38(1):33-59.
    PMID: 27678139 DOI: 10.1002/elps.201600331
    One of the most cited limitations of capillary (and microchip) electrophoresis is the poor sensitivity. This review continues to update this series of biennial reviews, first published in Electrophoresis in 2007, on developments in the field of on-line/in-line concentration methods in capillaries and microchips, covering the period July 2014-June 2016. It includes developments in the field of stacking, covering all methods from field amplified sample stacking and large volume sample stacking, through to isotachophoresis, dynamic pH junction, and sweeping. Attention is also given to on-line or in-line extraction methods that have been used for electrophoresis.
    MeSH terms: Cell Line; Humans; Inorganic Chemicals/analysis; Isoelectric Focusing; Organic Chemicals/analysis; Sensitivity and Specificity; Electrophoresis, Capillary/methods*; Microchip Analytical Procedures/methods*; Nanostructures/analysis; Capillary Electrochromatography; Isotachophoresis/methods*
  6. Yeap GY, Hrishikesan E, Chan YH, Mahmood WA
    J Fluoresc, 2017 Jan;27(1):105-110.
    PMID: 27679994 DOI: 10.1007/s10895-016-1938-5
    A new chalcone-based probe containing coumarin and naphthol at both ends has been synthesized via aldol condensation. The uniqueness of the newly derived probe can be ascribed to the presence of naphthol and coumarin units acting as binding site and signaling element, respectively. The fluorogenic behaviors toward various anions were investigated. The probe was characterized by various spectroscopic techniques and the in-depth study led to show excellent selectivity and sensitivity for fluoride ions. The hydrogen bonding thus formed with fluoride anion provides remarkable fluorometric responses. The interactions of the probe with fluoride ions were determined by fluorescence, FT-IR and NMR spectroscopic techniques. The exploratory studies by fluorescent spectral changes augur well for the naked-eye sensing applications.
  7. Coombs CM, Shields RY, Hunt EA, Lum YW, Sosnay PR, Perretta JS, et al.
    Acad Med, 2017 04;92(4):494-500.
    PMID: 27680320 DOI: 10.1097/ACM.0000000000001387
    PROBLEM: Because reported use of simulation in preclinical basic science courses is limited, the authors describe the design, implementation, and preliminary evaluation of a simulation-based clinical correlation curriculum in an anatomy course for first-year medical students at Perdana University Graduate School of Medicine (in collaboration with Johns Hopkins University School of Medicine).

    APPROACH: The simulation curriculum, with five weekly modules, was a component of a noncadaveric human anatomy course for three classes (n = 81 students) from September 2011 to November 2013. The modules were designed around major anatomical regions (thorax; abdomen and pelvis; lower extremities and back; upper extremities; and head and neck) and used various types of simulation (standardized patients, high-fidelity simulators, and task trainers). Several methods were used to evaluate the curriculum's efficacy, including comparing pre- versus posttest scores and comparing posttest scores against the score on 15 clinical correlation final exam questions.

    OUTCOMES: A total of 81 students (response rate: 100%) completed all pre- and posttests and consented to participate. Posttest scores suggest significant knowledge acquisition and better consistency of performance after participation in the curriculum. The comparison of performance on the posttests and final exam suggests that using simulation as an adjunctive pedagogy can lead to excellent short-term knowledge retention.

    NEXT STEPS: Simulation-based medical education may prove useful in preclinical basic science curricula. Next steps should be to validate the use of this approach, demonstrate cost-efficacy or the "return on investment" for educational and institutional leadership, and examine longer-term knowledge retention.

    MeSH terms: Simulation Training*; Adult; Anatomy/education*; Curriculum*; Education, Medical, Undergraduate/methods*; Educational Measurement; Female; Humans; Male; Patient Simulation*; Young Adult
  8. Lim LY, Mohd Firdaus CA, Fam XI, Goh EH
    J Comput Assist Tomogr, 2017 Jan;41(1):65-66.
    PMID: 27680416 DOI: 10.1097/RCT.0000000000000487
    Computed tomography (CT) is a widely used imaging modality. Although hyponatremia after CT imaging is rare, its effects can be devastating. Hyperosmolar radiocontrast acts as effective osmoles and causes fluid migration from intracellular into extracellular compartment. Dilutional hyponatremia will ensue if translocation of fluid is in excess of diuresis. This case report detailed an unusual case of acute symptomatic hyponatremia after CT renal protocol and the treatments given after its recognition.
    MeSH terms: Acute Disease; Contrast Media/administration & dosage; Contrast Media/adverse effects; Diagnosis, Differential; Humans; Hyponatremia/chemically induced*; Hyponatremia/diagnosis*; Hyponatremia/therapy; Iohexol/administration & dosage; Iohexol/adverse effects*; Male; Middle Aged; Saline Solution, Hypertonic/administration & dosage; Sodium, Dietary/therapeutic use; Tomography, X-Ray Computed/adverse effects*; Treatment Outcome; Symptom Assessment
  9. Rashidi Nodeh H, Wan Ibrahim WA, Kamboh MA, Sanagi MM
    Chemosphere, 2017 Jan;166:21-30.
    PMID: 27681257 DOI: 10.1016/j.chemosphere.2016.09.054
    A new graphene-based tetraethoxysilane-methyltrimethoxysilane sol-gel hybrid magnetic nanocomposite (Fe3O4@G-TEOS-MTMOS) was synthesised, characterized and successfully applied in magnetic solid-phase extraction (MSPE) for simultaneous analysis of polar and non-polar organophosphorus pesticides from several water samples. The Fe3O4@G-TEOS-MTMOS nanocomposite was characterized using Fourier transform-infrared spectroscopy, energy-dispersive X-ray spectroscopy, field emission scanning electron microscopy and X-ray diffraction. Separation, determination and quantification were achieved using gas chromatography coupled with micro electron capture detector. Adsorption capacity of the sorbent was calculated using Langmuir equation. MSPE was linear in the range 100-1000 pg mL(-1) for phosphamidon and dimethoate, and 10-100 pg mL(-1) for chlorpyrifos and diazinon, with limit of detection (S/N = 3) of 19.8, 23.7, 1.4 and 2.9 pg mL(-1) for phosphamidon, dimethoate, diazinon and chlorpyrifos, respectively. The LODs obtained is well below the maximum residual level (100 pg mL(-1)) as set by European Union for pesticides in drinking water. Acceptable precision (%RSD) was achieved for intra-day (1.3-8.7%, n = 3) and inter-day (7.6-17.8%, n = 15) analyses. Fe3O4@G-TEOS-MTMOS showed high adsorption capacity (54.4-76.3 mg g(-1)) for the selected OPPs. No pesticide residues were detected in the water samples analysed. Excellent extraction recoveries (83-105%) were obtained for the spiked OPPs from tap, river, lake and sea water samples. The newly synthesised Fe3O4@G-TEOS-MTMOS showed high potential as adsorbent for OPPs analysis.
    MeSH terms: Adsorption; Calibration; Chromatography, Gas; Diazinon/analysis; Dimethoate/analysis; Chlorpyrifos/analysis; Ferric Compounds/chemistry; Graphite/chemistry*; Magnetics*; Organic Chemicals/chemistry; Oxides/chemistry; Pesticide Residues/analysis*; Pesticides/analysis*; Phosphamidon/analysis; Phosphorus/chemistry; Silanes; Solvents/chemistry; X-Ray Diffraction; Spectroscopy, Fourier Transform Infrared; Phase Transition; Rivers; Solid Phase Extraction/methods*; Nanocomposites/chemistry*; Limit of Detection; Lakes/analysis
  10. Mumtaz A, Ashfaq UA, Ul Qamar MT, Anwar F, Gulzar F, Ali MA, et al.
    Nat Prod Res, 2017 Jun;31(11):1228-1236.
    PMID: 27681445 DOI: 10.1080/14786419.2016.1233409
    Medicinal plants are the main natural pools for the discovery and development of new drugs. In the modern era of computer-aided drug designing (CADD), there is need of prompt efforts to design and construct useful database management system that allows proper data storage, retrieval and management with user-friendly interface. An inclusive database having information about classification, activity and ready-to-dock library of medicinal plant's phytochemicals is therefore required to assist the researchers in the field of CADD. The present work was designed to merge activities of phytochemicals from medicinal plants, their targets and literature references into a single comprehensive database named as Medicinal Plants Database for Drug Designing (MPD3). The newly designed online and downloadable MPD3 contains information about more than 5000 phytochemicals from around 1000 medicinal plants with 80 different activities, more than 900 literature references and 200 plus targets. The designed database is deemed to be very useful for the researchers who are engaged in medicinal plants research, CADD and drug discovery/development with ease of operation and increased efficiency. The designed MPD3 is a comprehensive database which provides most of the information related to the medicinal plants at a single platform. MPD3 is freely available at: http://bioinform.info .
    MeSH terms: Online Systems; Plants, Medicinal/chemistry*; Drug Design; Databases, Factual*; Drug Discovery; Phytochemicals
  11. Houston SA, Ugusman A, Gnanadesikan S, Kennedy S
    Platelets, 2017 May;28(3):295-300.
    PMID: 27681689 DOI: 10.1080/09537104.2016.1218456
    Succinobucol is a phenolic antioxidant with anti-inflammatory and antiplatelet effects. Given the importance of oxidant stress in modulating platelet-platelet and platelet-vessel wall interactions, the aim of this study was to establish if antioxidant activity was responsible for the antiplatelet activity of succinobucol. Platelet aggregation in response to collagen and adenosine diphosphate (ADP) was studied in rabbit whole blood and platelet-rich plasma using impedance aggregometry. The effect of oxidant stress on aggregation, platelet lipid peroxides, and vascular tone was studied by incubating platelets, washed platelets or preconstricted rabbit iliac artery rings respectively with a combination of xanthine and xanthine oxidase (X/XO). To study the effect of succinobucol in vivo, anaesthetized rats were injected with up to 150 mg/kg succinobucol and aggregation measured in blood removed 15 mins later. Succinobucol (10-5-10-4M) significantly attenuated platelet aggregation to collagen and ADP in whole blood and platelet-rich plasma. X/XO significantly increased aggregation to collagen and platelet lipid peroxides and this was reversed by succinobucol. Addition of X/XO to denuded rabbit iliac arteries caused a dose-dependent relaxation which was significantly inhibited by succinobucol. In vivo administration up to 150 mg/kg had no effect on heart rate or mean arterial blood pressure but significantly inhibited platelet aggregation to collagen ex vivo. In conclusion, succinobucol displays anti-platelet activity in rabbit and rat blood and reverses the increase in platelet aggregation in response to oxidant stress.
    MeSH terms: Adenosine Diphosphate/pharmacology; Animals; Antioxidants/pharmacology*; Blood Platelets/cytology; Blood Platelets/drug effects*; Blood Pressure/drug effects; Blood Pressure/physiology; Collagen/antagonists & inhibitors; Collagen/pharmacology; Heart Rate/drug effects; Heart Rate/physiology; Iliac Artery/drug effects; Iliac Artery/physiology; Male; Myography; Platelet Aggregation/drug effects*; Platelet Aggregation Inhibitors/pharmacology*; Platelet Function Tests; Probucol/analogs & derivatives*; Probucol/pharmacology; Rabbits; Xanthine Oxidase/antagonists & inhibitors; Xanthine Oxidase/pharmacology; Rats, Sprague-Dawley; Xanthine/antagonists & inhibitors; Xanthine/pharmacology; Tissue Culture Techniques; Rats; Platelet-Rich Plasma/cytology
  12. Gartner CE, Lund KE, Barendregt JJ, Mohamed Nor N, Hassan H, Vedøy TF, et al.
    Eur J Public Health, 2017 Feb 1;27(1):139-144.
    PMID: 28177432 DOI: 10.1093/eurpub/ckw180
    Norway has achieved a noteworthy reduction in smoking prevalence over the past forty years. In 2015, 13% of Norwegians aged 13-74 smoked daily and a further 9% smoked occasionally. One of the objectives of the Norwegian 2013-16 national strategy for tobacco control is to achieve a reduction in the daily smoking prevalence to < 10% by 2016. This paper aims to estimate how long it will take for Norway to achieve the 10% smoking prevalence.
    MeSH terms: Humans; Norway; Smoking; Prevalence
  13. Mirzapour T, Tengku Ibrahim TAB, Movahedin M, Nowroozi MR
    Andrologia, 2017 Sep;49(7).
    PMID: 27682317 DOI: 10.1111/and.12700
    Destruction of spermatogonial stem cells (SSCs) along the chemotherapy and radiotherapy is one of the side effects of cancer treatments that lead to infertility. In vitro propagation of hSSCs is necessary to obtain an adequate number of cells for successful transplantation. In this study, hSSCs were isolated from testis biopsies of the patients with maturation arrest and proliferated in DMEM in the presence of LIF and bFGF for 5 weeks. The various types of human spermatogonia were identified in culture system and compared with testis tissue using morphological criteria at the ultrastructural level. The results showed that although many various types of spermatogonia were identified, but no remarkable difference was observed between spermatogonial cells in culture system and testis tissue. Electron and light microscopic studies of hSSC colonies did not show differentiated SSCs in the culture system. The results also showed that probably the suitable time for transplanting of SSCs in recipient testis is 2-3 weeks after culture. Because apoptosis which may affect the development of germ cells has not started in colony cells at this time and the population of apoptotic cells are low.
    MeSH terms: Adult; Cell Differentiation; Cells, Cultured; Humans; Infertility, Male/etiology*; Infertility, Male/pathology*; Male; Middle Aged; Neoplasms/therapy*; Sertoli Cells/ultrastructure; Testis/ultrastructure*; Time Factors; Apoptosis; Stem Cell Transplantation/methods; Flap Endonucleases; Microscopy, Electron, Transmission; Cell Proliferation; Adult Germline Stem Cells/drug effects; Adult Germline Stem Cells/radiation effects; Adult Germline Stem Cells/ultrastructure*
  14. Nurs Older People, 2017 08 31;29(7):10.
    PMID: 28856996 DOI: 10.7748/nop.29.7.10.s9
    1. ASEAN Conference on Healthy Ageing 10-12 October, Kuching, Malaysia tinyurl.com/ASEAN-conference.
  15. Shobugawa Y, Takeuchi T, Hibino A, Hassan MR, Yagami R, Kondo H, et al.
    Epidemiol Infect, 2017 Jan;145(2):272-284.
    PMID: 27682641
    In temperate zones, human respiratory syncytial virus (HRSV) outbreaks typically occur in cold weather, i.e. in late autumn and winter. However, recent outbreaks in Japan have tended to start during summer and autumn. This study examined associations of meteorological conditions with the numbers of HRSV cases reported in summer in Japan. Using data from the HRSV national surveillance system and national meteorological data for summer during the period 2007-2014, we utilized negative binomial logistic regression analysis to identify associations between meteorological conditions and reported cases of HRSV. HRSV cases increased when summer temperatures rose and when relative humidity increased. Consideration of the interaction term temperature × relative humidity enabled us to show synergistic effects of high temperature with HRSV occurrence. In particular, HRSV cases synergistically increased when relative humidity increased while the temperature was ⩾28·2 °C. Seasonal-trend decomposition analysis using the HRSV national surveillance data divided by 11 climate divisions showed that summer HRSV cases occurred in South Japan (Okinawa Island), Kyushu, and Nankai climate divisions, which are located in southwest Japan. Higher temperature and higher relative humidity were necessary conditions for HRSV occurrence in summer in Japan. Paediatricians in temperate zones should be mindful of possible HRSV cases in summer, when suitable conditions are present.
    MeSH terms: Humans; Humidity; Japan/epidemiology; Meteorological Concepts; Seasons; Temperature; Incidence; Respiratory Syncytial Virus, Human/isolation & purification*; Respiratory Syncytial Virus Infections/epidemiology*
  16. Kulchaitanaroaj P, Brooks JM, Chaiyakunapruk N, Goedken AM, Chrischilles EA, Carter BL
    J Hypertens, 2017 Jan;35(1):178-187.
    PMID: 27684354
    OBJECTIVE: To estimate long-term costs and outcomes attributable to a physician-pharmacist collaborative intervention compared with physician management alone for treating essential hypertension.

    METHODS: A Markov model cohort simulation with a 6-month cycle length to predict acute coronary syndrome, stroke, and heart failure throughout lifetime was performed. A cohort of 399 patients was obtained from two prospective, cluster randomized controlled clinical trials implementing physician-pharmacist collaborative interventions in community-based medical offices in the Midwest, USA. Framingham risk equations and other algorithms were used to predict the vascular diseases. SBP reduction due to the interventions deteriorated until 5 years. Direct medical costs using a payer perspective were adjusted to 2015 dollar value, and the main outcome was quality-adjusted life years (QALYs); both were discounted at 3%. The intervention costs were estimated from the trials, whereas the remaining parameters were from published studies. A series of sensitivity analyses including changing patient risks of vascular diseases, probabilistic sensitivity analysis, and a cost-effectiveness acceptability curve were performed.

    RESULTS: The lifetime incremental costs were $26 807.83 per QALY (QALYs gained = 0.14). The intervention provided the greatest benefit for the high-risk patients, moderate benefit for the trial patients, and the lowest benefit for the low-risk patients. If a payer is willing to pay $50 000 per QALY gained, in 48.6% of the time the intervention would be cost-effective.

    CONCLUSION: Team-based care such as a physician-pharmacist collaboration appears to be a cost-effective strategy for treating hypertension. The intervention is most cost-effective for high-risk patients.

    MeSH terms: Adult; Aged; Cost-Benefit Analysis; Female; Heart Failure; Humans; Hypertension/drug therapy*; Hypertension/economics*; Male; Middle Aged; Patient Care Team*; Pharmacists*; Physicians*; Prospective Studies; Health Care Costs*; Quality-Adjusted Life Years; Stroke
  17. Jayash SN, Hashim NM, Misran M, Baharuddin NA
    J Biomed Mater Res A, 2017 02;105(2):398-407.
    PMID: 27684563 DOI: 10.1002/jbm.a.35919
    The osteoprotegerin (OPG) system plays a critical role in bone remodelling by regulating osteoclast formation and activity. The study aimed to determine the physicochemical properties and biocompatibility of a newly formulated OPG-chitosan gel. The OPG-chitosan gel was formulated using human OPG protein and water-soluble chitosan. The physicochemical properties were determined using Fourier transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). Gel morphology was determined using scanning electron microscopy (SEM) and then it was subjected to a protein release assay and biodegradability test. An in vitro cytotoxicity test on normal human periodontal ligament (NHPL) fibroblasts and normal human (NH) osteoblasts was carried out using the AlamarBlue assay. In vivo evaluation in a rabbit model involved creating critical-sized defects in calvarial bone, filling with the OPG-chitosan gel and sacrificing at 12 weeks. In vitro results demonstrated that the 25 kDa OPG-chitosan gel had the highest rate of protein release and achieved 90% degradation in 28 days. At 12 weeks, the defects filled with 25 kDa OPG-chitosan gel showed significant (p 
    MeSH terms: Bone Regeneration*; Cell Line; Clergy; Female; Fibroblasts/metabolism; Fibroblasts/pathology; Humans; Osteoblasts/metabolism*; Osteoblasts/pathology; Periodontal Ligament/metabolism; Periodontal Ligament/pathology; Hydrogels/pharmacology; Hydrogels/chemistry
  18. Basri KN, Hussain MN, Bakar J, Sharif Z, Khir MFA, Zoolfakar AS
    Spectrochim Acta A Mol Biomol Spectrosc, 2017 Feb 15;173:335-342.
    PMID: 27685001 DOI: 10.1016/j.saa.2016.09.028
    Short wave near infrared spectroscopy (NIR) method was used to detect the presence of lard adulteration in palm oil. MicroNIR was set up in two different scan modes to study the effect of path length to the performance of spectral measurement. Pure and adulterated palm oil sample were classified using soft independent modeling class analogy (SIMCA) algorithm with model accuracy more than 0.95 reported for both transflectance and transmission modes. Additionally, by employing partial least square (PLS) regression, the coefficient of determination (R2) of transflectance and transmission were 0.9987 and 0.9994 with root mean square error of calibration (RMSEC) of 0.5931 and 0.6703 respectively. In order to remove the uninformative variables, variable selection using cumulative adaptive reweighted sampling (CARS) has been performed. The result of R2 and RMSEC after variable selection for transflectance and transmission were improved significantly. Based on the result of classification and quantification analysis, the transmission mode has yield better prediction model compared to the transflectance mode to distinguish the pure and adulterated palm oil.
    MeSH terms: Algorithms; Calibration; Dietary Fats/analysis; Food Contamination/analysis*; Signal Processing, Computer-Assisted; Least-Squares Analysis; Spectroscopy, Near-Infrared/instrumentation; Spectroscopy, Near-Infrared/methods*
  19. Wijedasa LS, Jauhiainen J, Könönen M, Lampela M, Vasander H, Leblanc MC, et al.
    Glob Chang Biol, 2017 03;23(3):977-982.
    PMID: 27670948 DOI: 10.1111/gcb.13516
  20. Hassan LEA, Iqbal MA, Dahham SS, Tabana YM, Ahamed MBK, Majid AMSA
    Anticancer Agents Med Chem, 2017;17(4):590-598.
    PMID: 27671298 DOI: 10.2174/1871520616666160926113711
    BACKGROUND: Cancer is characterized by uncontrolled cell division caused by dysregulation of cell proliferation. Therefore, agents that impair cancer cell proliferation could have potential therapeutic value. Higher plants are considered to be a good source of anticancer agents, and several clinically tested chemotherapeutic agents have been isolated from plants or derived from constituents of plant origin.

    METHODS: In the present study, a prenylated flavone (isoglabratephrin) was isolated from aerial parts of Tephrosia apollinea using a bioassay-guided technique. Chemical structure of the isolated compound was elucidated using spectroscopic techniques (NMR, IR, and LC-MC), elemental analysis and confirmed by using single crystal X-ray analysis. The antiproliferative effect of isoglabratephrin was tested using three human cancer cell lines (prostate (PC3), pancreatic (PANC-1), and colon (HCT-116) and one normal cell line (human fibroblast).

    RESULTS: Isoglabratephrin displayed selective inhibitory activity against proliferation of PC3 and PANC-1 cells with median inhibitory concentration values of 20.4 and 26.6 μg/ml, respectively. Isoglabratephrin demonstrated proapoptotic features, as it induced chromatin dissolution, nuclear condensation, and fragmentation. It also disrupted the mitochondrial membrane potential in the treated cancer cells.

    CONCLUSION: Isoglabratephrin could be a new lead to treat human prostate (PC3) and pancreatic (PANC-1) malignancies.

    MeSH terms: Antineoplastic Agents, Phytogenic/isolation & purification; Antineoplastic Agents, Phytogenic/pharmacology*; Antineoplastic Agents, Phytogenic/chemistry; Cells, Cultured; Dose-Response Relationship, Drug; Drug Screening Assays, Antitumor; Flavonoids/isolation & purification; Flavonoids/pharmacology*; Flavonoids/chemistry; Humans; Male; Models, Molecular; Pancreatic Neoplasms/drug therapy*; Pancreatic Neoplasms/pathology; Prostatic Neoplasms/drug therapy*; Prostatic Neoplasms/pathology; Structure-Activity Relationship; Colorectal Neoplasms/drug therapy*; Colorectal Neoplasms/pathology; Molecular Structure; Crystallography, X-Ray; Tephrosia/chemistry*; Cell Proliferation/drug effects
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