Protease is an enzyme that catalyses the hydrolysis of peptide bond in polypeptide chain and hold a wide range of applications in industry. The aim of this study is to clone and to express several genes encoding proteases from alkalitolerant bacteria Bacillus lehensis strain G1. A total of 13 genes encoding proteases have been selected using bioinformatics approach. These genes were then amplified using polymerase chain reaction (PCR) method. Subsequently, the PCR product was cloned into cloning vector pGEM®T easy and transformed into competent cell E. coli DH5α. The transformants were further verified by sequencing. The positive cloned were subcloned into the expression vector and were then expressed in Luria Bertani medium in the present of IPTG using E. coli BL21. The expressions of recombinant proteases were optimized for several hours at different temperatures, 16-37°C. Furthermore, structural prediction was performed using Modeller v9.18 for BleG1_1940. Each generated model was verified for overall completeness and bias, using PROCHECK, ERRAT, and Verify 3D. The overall quality of the model was relatively good with percentage of Ramachandran plot is 96.3%, PROCHECK is 86.2% and ERRAT score is 95%,.