Sains Malaysiana, 2013;42:1827-1829.

Abstract

DNA barcoding is the technique of sequencing a short fragment of cytochrome c oxidase subunit I (COI) mtDNA – a ‘DNA barcode’ - from a taxonomically unknown organism. This DNA barcode is then matched against a reference library of barcodes of known species origin to make a species identification. Since 2004 an international consortium has been promoting DNA barcoding as a global standard for taxonomic identifications (Savolainen et al. 2005). While we applaud Kumar et al. (2012) for bringing DNA barcoding to the attention of researchers in Malaysia, we have concerns about their understanding of the theory and methods of DNA barcoding.