Displaying publications 1 - 20 of 35 in total

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  1. A dose-response and meta-analysis of dehydroepiandrosterone (DHEA) supplementation on testosterone levels: perinatal prediction of randomized clinical trials
    Li Y, Ren J, Li N, Liu J, Tan SC, Low TY, et al.
    Exp Gerontol, 2020 11;141:111110.
    PMID: 33045358 DOI: 10.1016/j.exger.2020.111110
    BACKGROUND: Dehydroepiandrosterone (DHEA) has been aggressively sold as a dietary supplement to boost testosterone levels although the impact of DHEA supplementation on testosterone levels has not been fully established. Therefore, we performed a systematic review and meta-analysis of RCTs to investigate the effect of oral DHEA supplementation on testosterone levels.

    METHODS: A systematic literature search was performed in Scopus, Embase, Web of Science, and PubMed databases up to February 2020 for RCTs that investigated the effect of DHEA supplementation on testosterone levels. The estimated effect of the data was calculated using the weighted mean difference (WMD). Subgroup analysis was performed to identify the source of heterogeneity among studies.

    RESULTS: Overall results from 42 publications (comprising 55 arms) demonstrated that testosterone level was significantly increased after DHEA administration (WMD: 28.02 ng/dl, 95% CI: 21.44-34.60, p = 0.00). Subgroup analyses revealed that DHEA increased testosterone level in all subgroups, but the magnitude of increment was higher in females compared to men (WMD: 30.98 ng/dl vs. 21.36 ng/dl); DHEA dosage of ˃50 mg/d compared to ≤50 mg/d (WMD: 57.96 ng/dl vs. 19.43 ng/dl); intervention duration of ≤12 weeks compared to ˃12 weeks (WMD: 44.64 ng/dl vs. 19 ng/dl); healthy participants compared to postmenopausal women, pregnant women, non-healthy participants and androgen-deficient patients (WMD: 52.17 ng/dl vs. 25.04 ng/dl, 16.44 ng/dl and 16.47 ng/dl); and participants below 60 years old compared to above 60 years old (WMD: 31.42 ng/dl vs. 23.93 ng/dl).

    CONCLUSION: DHEA supplementation is effective for increasing testosterone levels, although the magnitude varies among different subgroups. More study needed on pregnant women and miscarriage.

  2. Fulltext An alternative peptone preparation using Hermetia illucens (Black soldier fly) hydrolysis: process optimization and performance evaluation
    Liu G, Tiang MF, Ma S, Wei Z, Liang X, Sajab MS, et al.
    PeerJ, 2024;12:e16995.
    PMID: 38426145 DOI: 10.7717/peerj.16995
    BACKGROUND: Hermetia illucens (HI), commonly known as the black soldier fly, has been recognized for its prowess in resource utilization and environmental protection because of its ability to transform organic waste into animal feed for livestock, poultry, and aquaculture. However, the potential of the black soldier fly's high protein content for more than cheap feedstock is still largely unexplored.

    METHODS: This study innovatively explores the potential of H. illucens larvae (HIL) protein as a peptone substitute for microbial culture media. Four commercial proteases (alkaline protease, trypsin, trypsase, and papain) were explored to hydrolyze the defatted HIL, and the experimental conditions were optimized via response surface methodology experimental design. The hydrolysate of the defatted HIL was subsequently vacuum freeze-dried and deployed as a growth medium for three bacterial strains (Staphylococcus aureus, Bacillus subtilis, and Escherichia coli) to determine the growth kinetics between the HIL peptone and commercial peptone.

    RESULTS: The optimal conditions were 1.70% w/w complex enzyme (alkaline protease: trypsin at 1:1 ratio) at pH 7.0 and 54 °C for a duration of 4 h. Under these conditions, the hydrolysis of defatted HIL yielded 19.25% ±0.49%. A growth kinetic analysis showed no significant difference in growth parameters (μmax, Xmax, and λ) between the HIL peptone and commercial peptone, demonstrating that the HIL hydrolysate could serve as an effective, low-cost alternative to commercial peptone. This study introduces an innovative approach to HIL protein resource utilization, broadening its application beyond its current use in animal feed.

  3. An updated checklist of the marine fish fauna of Redang Islands, Malaysia
    Du J, Loh KH, Hu W, Zheng X, Affendi YA, Ooi JLS, et al.
    Biodivers Data J, 2019;7:e47537.
    PMID: 31849564 DOI: 10.3897/BDJ.7.e47537
    Background: Redang Islands Marine Park consists of nine islands in the state of Terengganu, Malaysia. Redang Island is one of the largest off the east coast of Peninsular Malaysia, which is famous for its crystal-clear waters and white sandy beaches. The ichthyofauna of the Redang archipelago was surveyed by underwater visual observations between August 2016 and May 2018. Census data were compiled with existing records into the checklist of the marine fish of the Redang archipelago presented herein. A total of 314 species belonging to 51 families were recorded. The most speciose families (Pomacentridae, Labridae, Scaridae, Serranidae, Apogonidae, Carangidae, Gobiidae, Chaetodontidae, Lutjanidae, Nemipteridae and Siganidae) were also amongst the most speciose at the neighbouring Tioman archipelago (except Chaetodontidae). The coral fish diversity index value for the six families of coral reef fishes (Chaetodontidae, Pomacanthidae, Pomacentridae, Labridae, Scaridae and Acanthuridae) of the study sites was 132. We estimated that there were 427 coral reef fish species in the Redang archipelago. According to the IUCN Red List, eight species are Near Threatened (Carcharhinus melanopterus, Chaetodon trifascialis, Choerodon schoenleinii, Epinephelus fuscoguttatus, E. polyphekadion, Plectropomus leopardus, Taeniura lymma and Triaenodon obesus), eleven are Vulnerable (Bolbometopon muricatum, Chaetodon trifasciatus, Chlorurus sordidus, Dascyllus trimaculatus, Epinephelus fuscoguttatus, E. polyphekadion, Halichoeres marginatus, Heniochus acuminatus, Nebrius ferrugineus, Neopomacentrus cyanomos and Plectropomus areolatus) and three are Endangered (Amphiprion clarkia, Cheilinus undulatus and Scarus ghobban) in the Redang archipelago.

    New information: Five species are new records for Malaysia (Ctenogobiops mitodes, Epibulus brevis, Halichoeres erdmanni, H. richmondi and Scarus caudofasciatus) and 25 species are newly recorded in the Redang archipelago.

  4. Application of regression and artificial neural network analysis of Red-Green-Blue image components in prediction of chlorophyll content in microalgae
    Ying Ying Tang D, Wayne Chew K, Ting HY, Sia YH, Gentili FG, Park YK, et al.
    Bioresour Technol, 2023 Feb;370:128503.
    PMID: 36535615 DOI: 10.1016/j.biortech.2022.128503
    This study presented a novel methodology to predict microalgae chlorophyll content from colour models using linear regression and artificial neural network. The analysis was performed using SPSS software. Type of extractant solvents and image indexes were used as the input data for the artificial neural network calculation. The findings revealed that the regression model was highly significant, with high R2 of 0.58 and RSME of 3.16, making it a useful tool for predicting the chlorophyll concentration. Simultaneously, artificial neural network model with R2 of 0.66 and low RMSE of 2.36 proved to be more accurate than regression model. The model which fitted to the experimental data indicated that acetone was a suitable extraction solvent. In comparison to the cyan-magenta-yellow-black model in image analysis, the red-greenblue model offered a better correlation. In short, the estimation of chlorophyll concentration using prediction models are rapid, more efficient, and less expensive.
  5. Assessing the Redox Toxicity of 2D Nanosheets Based on Their Redox Effect on Cytochrome c in Microchannels
    Zhao L, Wang Q, Cui X, Li H, Zhao L, Wang Z, et al.
    Anal Chem, 2024 Feb 06;96(5):1913-1921.
    PMID: 38266028 DOI: 10.1021/acs.analchem.3c04062
    2D nanosheets (NSs) have been widely used in drug-related applications. However, a comprehensive investigation into the cytotoxicity mechanism linked to the redox activity is lacking. In this study, with cytochrome c (Cyt c) as the model biospecies, the cytotoxicity of 2D NSs was evaluated systematically based on their redox effect with microfluidic techniques. The interface interaction, dissolution, and redox effect of 2D NSs on Cyt c were monitored with pulsed streaming potential (SP) measurement and capillary electrophoresis (CE). The relationship between the redox activity of 2D NSs and the function of Cyt c was evaluated in vitro with Hela cells. The results indicated that the dissolution and redox activity of 2D NSs can be simultaneously monitored with CE under weak interface interactions and at low sample volumes. Both WS2 NSs and MoS2 NSs can reduce Cyt c without significant dissolution, with reduction rates measured at 6.24 × 10-5 M for WS2 NSs and 3.76 × 10-5 M for MoS2 NSs. Furthermore, exposure to 2D NSs exhibited heightened reducibility, which prompted more pronounced alterations associated with Cyt c dysfunction, encompassing ATP synthesis, modifications in mitochondrial membrane potential, and increased reactive oxygen species production. These observations suggest a positive correlation between the redox activity of 2D NSs and their redox toxicity in Hela cells. These findings provide valuable insight into the redox properties of 2D NSs regarding cytotoxicity and offer the possibility to modify the 2D NSs to reduce their redox toxicity for clinical applications.
  6. Association of body mass index and intestinal (faecal) Streptococcus in adults in Xining city, China P.R
    Ma M, Su J, Wang Y, Wang L, Li Y, Ding G, et al.
    Benef Microbes, 2022 Dec 07;13(6):465-472.
    PMID: 36264094 DOI: 10.3920/BM2021.0046
    Body mass index (BMI) and gut microbiota show significant interaction, but most studies on the relationship between BMI and gut microbiota have been done in Western countries. Relationships that are also identified in other cultural backgrounds are likely to have functional importance. Hence here we explore gut microbiota in adults living in Xining city (China P.R.) and relate results to subject BMI. Analysis of bacterial 16s rRNA gene was performed on faecal samples from participants with normal-weight (n=24), overweight (n=24), obesity (n=11) and type 2 diabetes (T2D) (n=8). The results show that unweighted but not weighted Unifrac distance was significantly different when gut microbiota composition was compared between the groups. Importantly, the genus Streptococcus was remarkably decreased in both obese subjects and subjects suffering from T2D, as compared to normal-weight subjects. Accordingly, strong association was identified between the genus Streptococcus and BMI and especially Streptococcus salivarius subsp. thermophiles was a major contributor in this respect. As previous studies have shown that Streptococcus salivarius subsp. thermophiles is also negatively associated with obesity in Western cohorts, our results suggest that this species is a potential probiotic for the prevention of obesity and related disorders.
  7. Asymmetric Synthesis of Arboduridine
    Yang R, Zhou Z, Jiang H, Kam TS, Chen K, Ma Z
    Angew Chem Int Ed Engl, 2024 Jan 15;63(3):e202316016.
    PMID: 38038685 DOI: 10.1002/anie.202316016
    The first asymmetric total synthesis of the monoterpenoid indole alkaloid arboduridine has been accomplished. The tricyclic A/B/D ring system was constructed by an enantioselective Michael reaction followed by intramolecular nucleophilic addition. Intramolecular α-amination of a ketone forged the piperidine ring, while a Horner-Wadsworth-Emmons (HWE) reaction was used to form the pyrrolidine ring. A reduction cyclization cascade led to formation of the tetrahydrofuran ring.
  8. Bifidobacterium infantis M-63 improves mental health in victims with irritable bowel syndrome developed after a major flood disaster
    Ma ZF, Yusof N, Hamid N, Lawenko RM, Mohammad WMZW, Liong MT, et al.
    Benef Microbes, 2019 Mar 13;10(2):111-120.
    PMID: 30525951 DOI: 10.3920/BM2018.0008
    Individuals in a community who developed irritable bowel syndrome (IBS) after major floods have significant mental health impairment. We aimed to determine if Bifidobacterium infantis M-63 was effective in improving symptoms, psychology and quality of life measures in flood-affected individuals with IBS and if the improvement was mediated by gut microbiota changes. Design was non-randomised, open-label, controlled before-and-after. Of 53 participants, 20 with IBS were given B. infantis M-63 (1×109 cfu/sachet/day) for three months and 33 were controls. IBS symptom severity scale, hospital anxiety and depression scale, SF-36 Questionnaire, hydrogen breath testing for small intestinal bacterial overgrowth and stools for 16S rRNA metagenomic analysis were performed before and after intervention. 11 of 20 who were given probiotics (M-63) and 20 of 33 controls completed study as per-protocol. Mental well-being was improved with M-63 vs controls for full analysis (P=0.03) and per-protocol (P=0.01) populations. Within-group differences were observed for anxiety and bodily pain (both P=0.04) in the M-63 per-protocol population. Lower ratio of Firmicutes/Bacteroidetes was observed with M-63 vs controls (P=0.01) and the lower ratio was correlated with higher post-intervention mental score (P=0.04). B. infantis M-63 is probably effective in improving mental health of victims who developed IBS after floods and this is maybe due to restoration of microbial balance and the gut-brain axis. However, our conclusion must be interpreted within the context of limited sample size. The study was retrospectively registered on 12 October 2017 and the Trial Registration Number (TRN) was NCT03318614.
  9. Biological active metabolites from microalgae for healthcare and pharmaceutical industries: A comprehensive review
    Ibrahim TNBT, Feisal NAS, Kamaludin NH, Cheah WY, How V, Bhatnagar A, et al.
    Bioresour Technol, 2023 Jan 21;372:128661.
    PMID: 36690215 DOI: 10.1016/j.biortech.2023.128661
    Microalgae are photoautotrophic microorganisms which comprise of species from several phyla. Microalgae are promising in producing a varieties of products, including food, feed supplements, chemicals, and biofuels. Medicinal supplements derived from microalgae are of a significant market in which compounds such as -carotene, astaxanthin, polyunsaturated fatty acids (PUFA) such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), and polysaccharides such as -glucan, are prominent. Microalgae species which are commonly applied for commercial productions include Isochrysis sp., Chaetoceros (Chlorella sp.), Arthrospira sp. (Spirulina Bioactive) and many more. In this present review, microalgae species which are feasible in metabolites production are being summarized. Metabolites produced by microalgae as well as their prospective applications in the healthcare and pharmaceutical industries, are comprehensively discussed. This evaluation is greatly assisting industrial stakeholders, investors, and researchers in making business decisions, investing in ventures, and moving the production of microalgae-based metabolites forward.
  10. Biomass waste as an alternative source of carbon and silicon-based absorbents for CO2 capturing application
    Suresh R, Gnanasekaran L, Rajendran S, Jalil AA, Soto-Moscoso M, Khoo KS, et al.
    Chemosphere, 2023 Dec;343:140173.
    PMID: 37714490 DOI: 10.1016/j.chemosphere.2023.140173
    The production of low-cost solid adsorbents for carbon dioxide (CO2) capture has gained massive consideration. Biomass wastes are preferred as precursors for synthesis of CO2 solid adsorbents, due to their high CO2 adsorption efficiency, and ease of scalable low-cost production. This review particularly focuses on waste biomass-derived adsorbents with their CO2 adsorption performances. Specifically, studies related to carbon (biochar and activated carbon) and silicon (silicates and geopolymers)-based adsorbents were summarized. The impact of experimental parameters including nature of biomass, synthesis route, carbonization temperature and type of activation methods on the CO2 adsorption capacities of biomass-derived pure carbon and silicon-based adsorbents were evaluated. The development of various enhancement strategies on biomass-derived adsorbents for CO2 capture and their responsible factors that impact adsorbent's CO2 capture proficiency were also reviewed. The possible CO2 adsorption mechanisms on the adsorbent's surface were highlighted. The challenges and research gaps identified in this research area have also been emphasized, which will help as further research prospects.
  11. Fulltext Cell-based influenza vaccine: current production, halal status assessment, and recommendations towards Islamic-compliant manufacturing
    Zulkarnain NN, Anuar N, Abd Rahman N, Sheikh Abdullah SR, Alias MN, Yaacob M, et al.
    Hum Vaccin Immunother, 2021 07 03;17(7):2158-2168.
    PMID: 33539195 DOI: 10.1080/21645515.2020.1865044
    Influenza virus is a life-threatening pathogen that infects millions of people every year, with annual mortality in the hundreds of thousands. The scenario for controlling infection has worsened with increasing numbers of vaccine hesitancy cases reported worldwide due to objections on safety, religious and other grounds. Uses of haram (impermissible) and mashbooh (doubtful) ingredients in vaccine production has raised doubts among Muslim consumers and consequently stimulated serious vaccine hesitancy. To address this major problem, we have reviewed and recommended some alternatives appropriate for manufacturing cell-based influenza vaccine which comply with Islamic laws and consumers' needs. Intensive assessments of current influenza vaccine production in both scientific and Islamic views have led to the identification of four main ingredients deemed impermissible in novel sharia-compliant (approved by Islamic laws) vaccine manufacturing. Only some of these impermissible components could be replaced with halal (permissible) alternatives, while others remain impermissible due to unavailability and unsuitability.
  12. Complete genome sequence of Bacillus Licheniformis NWMCC0046, a candidate for the laundry industry
    Zhou W, Zeng S, Yu J, Xiang J, Zhang F, Takriff MS, et al.
    J Basic Microbiol, 2023 Feb;63(2):223-234.
    PMID: 36538731 DOI: 10.1002/jobm.202200528
    In this study, selected properties of protease and the complete genome sequence of Bacillus licheniformis NWMCC0046 were investigated, to discover laundry applications and other potential probiotic properties of this strain. Partial characterization of B. licheniformis NWMCC0046 showed that its protease has good activity both in alkaline environments and at low temperatures. Also, the protease is compatible with commercial detergents and can be used as a detergent additive for effective stain removal at low temperatures. The complete genome sequence of B. licheniformis NWMCC0046 is comprised of a 4,321,565 bp linear chromosome with a G + C content of 46.78% and no plasmids. It had 4504 protein-encoding genes, 81 transfer RNA (tRNA) genes, and 24 ribosomal RNA (rRNA) genes. Genomic analysis revealed genes involved in exocellular enzyme production and probiotic properties. In addition, genomic sequence analysis revealed specific genes encoding carbohydrate metabolism pathways, resistance, and cold adaptation capacity. Overall, protease properties show its potential as a detergent additive enzyme. The complete genome sequence information of B. licheniformis NWMCC0046 was obtained, and functional prediction revealed its numerous probiotic properties.
  13. Fulltext Deterioration of left ventricular systolic function in extended Pacing to Avoid Cardiac Enlargement (PACE) trial: the predictive value of early systolic dyssynchrony
    Fang F, Luo XX, Zhang Q, Azlan H, Razali O, Ma Z, et al.
    Europace, 2015 Oct;17 Suppl 2:ii47-53.
    PMID: 26842115 DOI: 10.1093/europace/euv130
    Biventricular (BiV) pacing was superior to right ventricular apical (RVA) pacing at extended follow-up in the Pacing to Avoid Cardiac Enlargement (PACE) trial. Early pacing-induced systolic dyssynchrony (DYS) might be related to mid-term result. However, it remains unknown whether early pacing-induced DYS can predict long-term reduction of left ventricular (LV) systolic function.
  14. Divergent Synthesis of Skeletally Distinct Arboridinine and Arborisidine
    Wang C, Pang Y, Wu Y, Zhang N, Yang R, Li Y, et al.
    Angew Chem Int Ed Engl, 2021 12 20;60(52):26978-26985.
    PMID: 34665909 DOI: 10.1002/anie.202110149
    A divergent synthesis of skeletally distinct arboridinine and arborisidine was achieved. The central divergent strategy was inspired by the divergent biosynthetic cyclization mode of arboridinine and arborisidine and their hidden topological connection. The branch point was reached through a Michael and Mannich cascade process. A site-selective intramolecular Mannich reaction was developed to construct the tetracyclic core of arboridinine, while a site-selective intramolecular α-amination of ketone was used to access the tetracyclic core of arborisidine. A strategic Peterson olefination through intramolecular nucleophile delivery was able to set up the exocyclic olefin of arboridinine.
  15. Fulltext Donkey whey protein and peptides regulate gut microbiota community and physiological functions of D-galactose-induced aging mice
    Zhou X, Tian X, Song L, Luo L, Ma Z, Zhang F
    Food Sci Nutr, 2023 Feb;11(2):752-764.
    PMID: 36789044 DOI: 10.1002/fsn3.3111
    The prolongation of life span has attracted more and more attention in the current world. Gut microbiota is considered one of the most critical elements and is essential in regulating life span and quality. The effects of donkey whey protein (DWP) and donkey whey hydrolysate (DWPP) on physiological functions and gut microbiota of D-galactose-induced aging mice were investigated to find new strategies for resisting aging. Our results showed that DWP and DWPP could increase the body weight gain velocity, superoxide dismutase (SOD) activity, and thymus index, whereas decrease the level of reactive oxygen species (ROS) and malondialdehyde (MDA), and improve the aging of the body in the liver congestion, oozy draw focal sclerosis of chronic inflammation. The effects of medium and high concentrations of DWP and low and medium concentrations of DWPP were the same as the vitamin C (Vc)-positive control group. It was found that both DWP and DWPP could change α-diversity; the relative abundance of Lactobacillus increased, whereas the relative abundance of Helicobacter and Stenotrophomonas decreased after being treated with DWP and DWPP. The correlation between intestinal microflora and physiological indexes showed that chao1, ACE, and observed species indexes in the α index were positively correlated with weight gain velocity, SOD activity, and thymus index. The relative abundance of Lactobacillus was positively correlated with SOD and thymus index but negatively correlated with MDA. The relative abundance of Stenotrophomonas was opposite to that of Lactobacillus. The Anaerobiospirillum, Fusobacterium, and Dubosiella had a significant positive correlation with the weight gain velocity. The study provided a deeper more profound understanding of the potential use of DWP and DWPP in senescence delays.
  16. Fulltext Effects of different types and doses of whey protein on the physiological and intestinal flora in D-galactose induced aging mice
    Ma Z, Zhang F, Ma H, Chen X, Yang J, Yang Y, et al.
    PLoS One, 2021;16(4):e0248329.
    PMID: 33857162 DOI: 10.1371/journal.pone.0248329
    The elderly usually suffer from many diseases. Improving the quality of life of the elderly is an urgent social issue. In this present study, D-galactose treated aging mice models were used to reveal the effects of different animal sources and different doses of whey protein (WP) on the immune indexes organs and intestinal flora. A total of 9 groups were set up, including normal control (NC), negative control (NS), positive control (Vc), low-, medium- and high-doses of cow WP intervention groups (CL, CM and CH for short, correspondingly) and low-, medium- and high-doses of goat WP intervention groups (GL, GM and GH for short, correspondingly). The body weight gain, thymus/body weight ratio, superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, spleen immunoglobulins G (IgG), spleen interleukin-2 (IL-2) and spleen interleukin-2 (IL-6) were measured. Then, the intestinal contents were collected, and 16s genes of intestinal bacteria were sequenced to reveal the changes in bacterial flora structure. WP intervention significantly increased the weight gain, thymus/body ratio and SOD activity, but decrease the content of MDA. WP intervention increased some immune indicators. All the WP treated aging mice showed similar values of physiological indexes to that of the Vc group, even better. The relative abundance of Lactobacillus and Stenotrophomonas was increased and decreased, respectively, by both cow and goat WP. Lactobacillus may be involved in regulating the functional repair of organisms. In contrast, Stenotrophomonas might play a negative role in the immune and antioxidant capacity of the body. Combining physiological indicators and intestinal flora structure, low-concentration WP for cow and goat might be optimal for aging models.
  17. Fulltext Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
    Klionsky DJ, Abdelmohsen K, Abe A, Abedin MJ, Abeliovich H, Acevedo Arozena A, et al.
    Autophagy, 2016;12(1):1-222.
    PMID: 26799652 DOI: 10.1080/15548627.2015.1100356
  18. Fulltext Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1
    Klionsky DJ, Abdel-Aziz AK, Abdelfatah S, Abdellatif M, Abdoli A, Abel S, et al.
    Autophagy, 2021 Jan;17(1):1-382.
    PMID: 33634751 DOI: 10.1080/15548627.2020.1797280
    In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.
  19. Hydrogen production and pollution mitigation: Enhanced gasification of plastic waste and biomass with machine learning & storage for a sustainable future
    Bin Abu Sofian ADA, Lim HR, Chew KW, Khoo KS, Tan IS, Ma Z, et al.
    Environ Pollut, 2024 Feb 01;342:123024.
    PMID: 38030108 DOI: 10.1016/j.envpol.2023.123024
    The pursuit of carbon neutrality confronts the twofold challenge of meeting energy demands and reducing pollution. This review article examines the potential of gasifying plastic waste and biomass as innovative, sustainable sources for hydrogen production, a critical element in achieving environmental reform. Addressing the problem of greenhouse gas emissions, the work highlights how the co-gasification of these feedstocks could contribute to environmental preservation by reducing waste and generating clean energy. Through an analysis of current technologies, the potential for machine learning to refine gasification for optimal hydrogen production is revealed. Additionally, hydrogen storage solutions are evaluated for their importance in creating a viable, sustainable energy infrastructure. The economic viability of these production methods is critically assessed, providing insights into both their cost-effectiveness and ecological benefits. Findings indicate that machine learning can significantly improve process efficiencies, thereby influencing the economic and environmental aspects of hydrogen production. Furthermore, the study presents the advancements in these technologies and their role in promoting a transition to a green economy and circular energy practices. Ultimately, the review delineates how integrating hydrogen production from unconventional feedstocks, bolstered by machine learning and advanced storage, can contribute to a sustainable and pollution-free future.
  20. Fulltext Improvement of chicken plasma protein hydrolysate angiotensin I-converting enzyme inhibitory activity by optimizing plastein reaction
    Gao D, Guo P, Cao X, Ge L, Ma H, Cheng H, et al.
    Food Sci Nutr, 2020 Jun;8(6):2798-2808.
    PMID: 32566197 DOI: 10.1002/fsn3.1572
    Chicken plasma protein hydrolysate (CPPH) was prepared by trypsin with angiotensin I-converting enzyme (ACE) inhibitory activity of 53.5% ± 0.14% and the degree of hydrolysis (DH) of 16.22% ± 0.21% at 1 mg·ml-1; then, five proteases, including pepsin, trypsin, papain, alcalase, and neutrase, were employed to improve ACE inhibitory ability by catalyzing plastein reaction. The results indicated that trypsin-catalyzed plastein reaction showed the highest ACE inhibitory activity. The exogenous amino acids of leucine, histidine, tyrosine, valine, and cysteine were selected to modify the CPPH. The leucine-modified plastein reaction released the highest ACE inhibitory activity. The effects of four reaction parameters on plastein reaction were studied, and the optimal conditions with the purpose of obtaining the most powerful ACE inhibitory peptides from modified products were obtained by response surface methodology (RSM). The maximum ACE inhibition rate of the modified hydrolysate reached 82.07% ± 0.03% prepared at concentration of hydrolysates of 30%, reaction time of 4.9 hr, pH value of 8.0, temperature of 40°C, and E/S ratio of 5,681.62 U·g-1. The results indicated that trypsin-catalyzed plastein reaction increased ACE inhibitory activity of chicken plasma protein hydrolysates by 28.57%.
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