Displaying publications 1 - 20 of 83 in total

Abstract:
Sort:
  1. Fulltext Correlation of Foot Bimalleolar Angle and Ultrasonography in Assessing the Severity of Club Foot in Neonates Treated by the Ponseti Method
    Bajaj V, Anshuman R, Verma N, Singh MP, Tandon A
    Malays Orthop J, 2018 Nov;12(3):14-18.
    PMID: 30555641 DOI: 10.5704/MOJ.1811.003
    Introduction: Correlation of Pirani score and foot bimalleolar (FBM) angle has been used in few studies but correlation of FBM angle with ultrasonography has never been evaluated so they are being correlated in assessing the severity of clubfoot in neonates treated by Ponseti method. Material and Methods: Thirty-two feet with congenital talipes equinovarus (CTEV) deformity in neonates were prospectively treated by the Ponseti method. FBM angle and ultrasound parameters were measured three times i.e. at the time of initial presentation, at four weeks of treatment and at completion of treatment. The feet were divided according to the Pirani score in groups: one (0-2.0), two (2.5-4) and three (4.5-6). Correlation between FBM angle and ultrasound parameters were evaluated using Pearson correlation/regression. Results: Correlation between FBM angle and ultrasound parameters were statistically significant (p-value < 0.05). Conclusion: Ultrasound has the potential to accurately depict the pathoanatomy in clubfoot. FBM angle and ultrasound are objective methods to assess the severity of clubfoot. FBM angle and ultrasonography correlated in severity of deformity and correction achieved along the course of treatment.
  2. Uncovering the complex role of interferon-gamma in suppressing type 2 immunity to cancer
    Bhat AA, Goyal A, Thapa R, Almalki WH, Kazmi I, Alzarea SI, et al.
    Cytokine, 2023 Nov;171:156376.
    PMID: 37748333 DOI: 10.1016/j.cyto.2023.156376
    Cancer involves cells' abnormal growth and ability to invade or metastasize to different body parts. Cancerous cells can divide uncontrollably and spread to other areas through the lymphatic or circulatory systems. Tumors form when malignant cells clump together in an uncontrolled manner. In this context, the cytokine interferon-gamma (IFN-γ) is crucial in regulating immunological responses, particularly malignancy. While IFN-γ is well-known for its potent anti-tumor effects by activating type 1 immunity, recent research has revealed its ability to suppress type 2 immunity, associated with allergy and inflammatory responses. This review aims to elucidate the intricate function of IFN-γ in inhibiting type 2 immune responses to cancer. We explore how IFN-γ influences the development and function of immune cells involved in type 2 immunity, such as mast cells, eosinophils, and T-helper 2 (Th2) cells. Additionally, we investigate the impact of IFN-mediated reduction of type 2 immunity on tumor development, metastasis, and the response to immunotherapeutic interventions. To develop successful cancer immunotherapies, it is crucial to comprehend the complex interplay between type 2 and type 1 immune response and the regulatory role of IFN-γ. This understanding holds tremendous promise for the development of innovative treatment approaches that harness the abilities of both immune response types to combat cancer. However, unraveling the intricate interplay between IFN-γ and type 2 immunity in the tumor microenvironment will be essential for achieving this goal.
  3. Partial superficial parotidectomy for pleomorphic adenoma of the parotid gland
    Bhattacharya A, Singh M, Shah A, Varghese LL
    BMJ Case Rep, 2021 Jun 14;14(6).
    PMID: 34127499 DOI: 10.1136/bcr-2020-238759
    Pleomorphic adenoma, otherwise called as benign mixed tumour, is the most common salivary gland tumour which accounts for 60% of all benign salivary gland tumours. The clinical, radiological and histopathological presentations are varied. The tumour occurs in diverse anatomical sites and can consist of epithelial and mesenchymal components. In this case report, the patient reported with an asymptomatic swelling on the face. CT scan with contrast was advised. The clinical, roentgenographic findings and Fine Needle Aspiration Cytology were indicative of pleomorphic adenoma of the parotid gland. Treatment included partial superficial parotidectomy under general anaesthesia using the modified Blair's incision. The facial nerve was not involved. Part of the gland along with the tumour was resected completely superficial to the facial nerve with a margin of normal tissue all around. Histopathologic examination of tissue specimen confirmed the lesion as pleomorphic adenoma. The patient was asymptomatic at 6-month follow-up.
  4. Post-mortem pulmonary tuberculosis: comparison of available diagnostic methods
    Chainchel Singh MK, Johari B, Naik VR, Lai PS, Siew SF
    Trop Biomed, 2023 Jun 01;40(2):165-169.
    PMID: 37650402 DOI: 10.47665/tb.40.2.006
    Tuberculosis (TB) caused by Mycobacterium tuberculosis remains a serious public health threat with the World Health Organisation (WHO) reporting 5.8 million cases and 1.3 million deaths in the year 2020 due to TB. TB can be diagnosed by imaging, histopathological and bacteriological methods with culture remaining the gold standard. This study was performed to look at the sensitivity and specificity of post-mortem computed tomography (PMCT) imaging when compared to culture in diagnosing pulmonary tuberculosis. This was a retrospective comparative study looking at post mortem cases where lung tissue samples sent for TB culture at Hospital Kuala Lumpur were compared against PMCT imaging. Exclusion criteria included contaminated samples, decomposed cases, immunocompromised subjects and those below 18 years of age. Subjects included 80 medico-legal autopsy cases at the National Institute of Forensic Medicine, Hospital Kuala Lumpur, Malaysia who had whole body PMCT done in accordance with the Institute's protocol and tissue samples sent for bacteriology culture for tuberculosis. PMCT findings were positively associated with acid-fast organisms in 23.5 out of 33 cases (71.2%). Our study also showed that PMCT had a sensitivity of 71.3% and specificity of 54.3% (95% CI: 39.5-68.4) in diagnosing TB based on the protocol set in this study. This study showed that there was relatively good agreement between radiological PMCT findings and bacterial culture, suggesting that radiological examination is a relatively reliable tool for preliminary screening and possible diagnosis of TB prior to a postmortem examination which would be beneficial in reducing the risk of transmission of TB to health workers during autopsy.
  5. The first isolate of Mycoplasma pneumoniae in Malaysia
    Cheong YM, Jegathesan M, Singh M, Wong S, Ong L
    Malays J Pathol, 1985 Aug;7:51-2.
    PMID: 3939616
  6. Harm reduction in Asia: a successful response to hidden epidemics. The Asian Harm Reduction Network
    Crofts N, Costigan G, Narayanan P, Gray J, Dorabjee J, Langkham B, et al.
    AIDS, 1998;12 Suppl B:S109-15.
    PMID: 9679636
  7. Fulltext Stochastic Leader Gravitational Search Algorithm for Enhanced Adaptive Beamforming Technique
    Darzi S, Islam MT, Tiong SK, Kibria S, Singh M
    PLoS One, 2015;10(11):e0140526.
    PMID: 26552032 DOI: 10.1371/journal.pone.0140526
    In this paper, stochastic leader gravitational search algorithm (SL-GSA) based on randomized k is proposed. Standard GSA (SGSA) utilizes the best agents without any randomization, thus it is more prone to converge at suboptimal results. Initially, the new approach randomly choses k agents from the set of all agents to improve the global search ability. Gradually, the set of agents is reduced by eliminating the agents with the poorest performances to allow rapid convergence. The performance of the SL-GSA was analyzed for six well-known benchmark functions, and the results are compared with SGSA and some of its variants. Furthermore, the SL-GSA is applied to minimum variance distortionless response (MVDR) beamforming technique to ensure compatibility with real world optimization problems. The proposed algorithm demonstrates superior convergence rate and quality of solution for both real world problems and benchmark functions compared to original algorithm and other recent variants of SGSA.
  8. Fulltext Enhanced efficacy of β-carotene loaded solid lipid nanoparticles optimized and developed via central composite design on breast cancer cell lines
    Dutta RS, Elhassan GO, Devi TB, Bhattacharjee B, Singh M, Jana BK, et al.
    Heliyon, 2024 Apr 15;10(7):e28457.
    PMID: 38586388 DOI: 10.1016/j.heliyon.2024.e28457
    β-carotene is obtained from both plants and animals and has been the subject of intense research because of its provitamin-A, antioxidant, and anticancer effects. Its limited absorption and oxidative degradation significantly reduce its antitumor efficacy when taken orally. In our study, we utilize a central composite design to develop "bio-safe and highly bio-compatible" solid lipid nanoparticles (SLNs) by using only the combination of palmitic acid and poloxamer-407, a block co-polymer as a surfactant. The current research aim to develop and characterize SLNs loaded with β-carotene to improve their bioavailability and therapeutic efficacy. In addition, the improved cytotoxicity of solid lipid nanoparticles loaded with β-carotene was screened in-vitro in human breast cancer cell lines (MCF-7). The nanoparticles exhibits good stability, as indicated by their mean zeta potential of -26.3 ± 1.3 mV. The particles demonstrated high drug loading and entrapment capabilities. The fabricated nanoparticle's prolonged release potential was shown by the in-vitro release kinetics, which showed a first-order release pattern that adhered to the Higuchi model and showed a slow, linear, and steady release over 48 h. Moreover, a diffusion-type release mechanism was used to liberate β-carotene from the nanoparticles. For six months, the nanoparticles also showed a notable degree of physical stability. Lastly, using the MTT assay, the anti-cancer properties of β-carotene-loaded solid lipid nanoparticles were compared with intact β-carotene on MCF-7 cell lines. The cytotoxicity tests have shown that the encapsulation of β-carotene in the lipid bilayers of the optimized formulation does not interfere with the anti-cancer activity of the drug. When compared to standard β-carotene, β-carotene loaded SLNs showed enhanced anticancer efficacy and it is a plausible therapeutic candidate for enhancing the solubility of water-insoluble and degradation-sensitive biotherapeutics like β-carotene.
  9. Fulltext Metamaterial-Embedded Low SAR PIFA for Cellular Phone
    Faruque MR, Hossain MI, Misran N, Singh M, Islam MT
    PLoS One, 2015;10(11):e0142663.
    PMID: 26599584 DOI: 10.1371/journal.pone.0142663
    A metamaterial-embedded planar inverted-F antenna (PIFA) is proposed in this study for cellular phone applications. A dual-band PIFA is designed to operate both GSM 900 MHz and DCS 1800 MHz. The ground plane of a conventional PIFA is modified using a planar one-dimensional metamaterial array. The investigation is performed using the Finite Integration Technique (FIT) of CST Microwave Studio. The performance of the developed antenna was measured in an anechoic chamber. The specific absorption rate (SAR) values are calculated considering two different holding positions: cheek and tilt. The SAR values are measured using COMOSAR measurement system. Good agreement is observed between the simulated and measured data. The results indicate that the proposed metamaterial-embedded antenna produces significantly lower SAR in the human head compared to the conventional PIFA. Moreover, the modified antenna substrate leads to slight improvement of the antenna performances.
  10. Analysis of differentially expressed proteins in cancerous and normal colonic tissues
    Gam LH, Leow CH, Man CN, Gooi BH, Singh M
    World J Gastroenterol, 2006 Aug 21;12(31):4973-80.
    PMID: 16937492
    AIM: To identify and analyze the differentially expressed proteins in normal and cancerous tissues of four patients suffering from colon cancer.

    METHODS: Colon tissues (normal and cancerous) were homogenized and the proteins were extracted using three protein extraction buffers. The extraction buffers were used in an orderly sequence of increasing extraction strength for proteins with hydrophobic properties. The protein extracts were separated using the SDS-PAGE method and the images were captured and analyzed using Quantity One software. The target protein bands were subjected to in-gel digestion with trypsin and finally analyzed using an ESI-ion trap mass spectrometer.

    RESULTS: A total of 50 differentially expressed proteins in colonic cancerous and normal tissues were identified.

    CONCLUSION: Many of the identified proteins have been reported to be involved in the progression of similar or other types of cancers. However, some of the identified proteins have not been reported before. In addition, a number of hypothetical proteins were also identified.

  11. Green synthesis of silver nanoparticles using Ocimum sanctum Linn. and its antibacterial activity against multidrug resistant Acinetobacter baumannii
    Gautam D, Dolma KG, Khandelwal B, Gupta M, Singh M, Mahboob T, et al.
    PeerJ, 2023;11:e15590.
    PMID: 37529215 DOI: 10.7717/peerj.15590
    The biosynthesis of nanoparticles using the green route is an effective strategy in nanotechnology that provides a cost-effective and environmentally friendly alternative to physical and chemical methods. This study aims to prepare an aqueous extract of Ocimum sanctum (O. sanctum)-based silver nanoparticles (AgNPs) through the green route and test their antibacterial activity. The biosynthesized silver nanoparticles were characterised by colour change, UV spectrometric analysis, FTIR, and particle shape and size morphology by SEM and TEM images. The nanoparticles are almost spherical to oval or rod-shaped with smooth surfaces and have a mean particle size in the range of 55 nm with a zeta potential of -2.7 mV. The antibacterial activities of AgNPs evaluated against clinically isolated multidrug-resistant Acinetobacter baumannii (A. baumannii) showed that the AgNPs from O. sanctum are effective in inhibiting A. baumannii growth with a zone of inhibition of 15 mm in the agar well diffusion method and MIC and MBC of 32 µg/mL and 64 µg/mL, respectively. The SEM images of A. baumannii treated with AgNPs revealed damage and rupture in bacterial cells. The time-killing assay by spectrophotometry revealed the time- and dose-dependent killing action of AgNPs against A. baumannii, and the assay at various concentrations and time intervals indicated a statistically significant result in comparison with the positive control colistin at 2 µg/mL (P 
  12. Fulltext Acute Paraplegia in a Toddler: A Diagnostic Journey Compounding the Challenge in Management: A Case Report
    Goh JH, Fazir M, Zainal-Abidin NA, Amir D, Singh M
    Malays Orthop J, 2016 Jul;10(2):56-60.
    PMID: 28435564 MyJurnal DOI: 10.5704/MOJ.1607.012
    Spinal tuberculosis is not common in the paediatric age group. Initial clinical features are often vague and non specific until the disease progresses to later stages. We highlight the diagnostic difficulties and management challenges of a complicated extradural tuberculoma with neurological deficits in a very young girl.
  13. Alginate-based bipolymeric-nanobioceramic composite matrices for sustained drug release
    Hasnain MS, Nayak AK, Singh M, Tabish M, Ansari MT, Ara TJ
    Int J Biol Macromol, 2016 Feb;83:71-7.
    PMID: 26608007 DOI: 10.1016/j.ijbiomac.2015.11.044
    Alginate-based bipolymeric-nanobioceramic composite matrices for sustained drug release were developed through incorporation of nano-hydroxyapatite [nHAp] powders within ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems. nHAp powders were synthesized by precipitation technique using calcium hydroxide [Ca(OH)2] and orthophosphoric acid [H3PO4] as raw materials. The average particle size of these was synthesized. nHAp powders was found as 19.04 nm and used to prepare nHAp-alginate-PVP beads containing DS. These beads exhibited drug entrapment efficiency (%) of 65.82±1.88 to 94.45±3.72% and average bead sizes of 0.98±0.07 to 1.23±0.15 mm. These beads were characterized by scanning electron microscopy (SEM) and Fourier transform-infra red (FTIR) spectroscopy analyses. Various nHAp-alginate-PVP beads containing DS exhibited prolonged sustained drug release and followed the Koresmeyer-Peppas model of drug release (R2=0.9908-0.9978) with non-Fickian release (anomalous transport) mechanism (n=0.73-0.84) for drug release over 8 h.
  14. QbD-Driven Development and Validation of Liquid Chromatography Tandem Mass Spectrometric Method for the Quantitation of Sildenafil in Human Plasma
    Hasnain MS, Ansari SA, Rao S, Tabish M, Singh M, Abdullah MS, et al.
    J Chromatogr Sci, 2017 Jul 01;55(6):587-594.
    PMID: 28335023 DOI: 10.1093/chromsci/bmx010
    The present work was employing the Quality by Design approach for the development and validation of a LC-MS-MS method to support the clinical advancement in determination of sildenafil in human plasma using lorazepam as an internal standard. Sample preparation involved solid phase extraction and calibration range observed between 3 and 1,700 ng/mL. The method was systematically optimized by employing Box-Behnken design and used mobile phase flow rate, pH and composition of mobile phase as the critical factors, and assessing the design for retention time and peak area as the responses. A substantial decrease in the variability associated with the method variables was shown in optimization studies and confirmed enhanced method robustness. The present studies revealed that developed method achieves all the regulatory requirements for linearity, accuracy, precision, selectivity, sensitivity and stability for the determination of sildenafil in human plasma. There was not any significant change in the stability of the drug shown by stability studies, performed in human plasma through freeze-thaw cycles, bench-top stability, short-term stability, long-term stability and auto sampler stability. In short, this method shows satisfactory results for the analysis of sildenafil in human plasma and possesses high degree of utility in pharmacokinetic and bioequivalence studies.
  15. Studies on the Malayan forest rat filaria, Breinlia booliati (Filarioidea: Onchocercidae): transmission to laboratory rats
    Ho BC, Singh M, Yap EH, Lim BL
    Int J Parasitol, 1976 Apr;6(2):113-6.
    PMID: 1262122
  16. Observations on the development of a new filaria (Breinlia booliati, Singh and Ho, 1973) of a rat Rattus sabanus in the mosquito Aedes togoi
    Ho BC, Singh M, Lim BL
    J Helminthol, 1973;47(2):135-40.
    PMID: 4724313
  17. Fulltext A Polarization Independent Quasi-TEM Metamaterial Absorber for X and Ku Band Sensing Applications
    Hoque A, Tariqul Islam M, Almutairi AF, Alam T, Jit Singh M, Amin N
    Sensors (Basel), 2018 Nov 30;18(12).
    PMID: 30513675 DOI: 10.3390/s18124209
    In this paper, a dual-band metamaterial absorber (MMA) ring with a mirror reflexed C-shape is introduced for X and Ku band sensing applications. The proposed metamaterial consists of two square ring resonators and a mirror reflexed C-shape, which reveals two distinctive absorption bands in the electromagnetic wave spectrum. The mechanism of the two-band absorber particularly demonstrates two resonance frequencies and absorption was analyzed using a quasi-TEM field distribution. The absorption can be tunable by changing the size of the metallic ring in the frequency spectrum. Design and analysis of the proposed meta-absorber was performed using the finite-integration technique (FIT)-based CST microwave studio simulation software. Two specific absorption peaks value of 99.6% and 99.14% are achieved at 13.78 GHz and 15.3 GHz, respectively. The absorption results have been measured and compared with computational results. The proposed dual-band absorber has potential applications in sensing techniques for satellite communication and radar systems.
  18. Fulltext Pan-Asian adapted ESMO Clinical Practice Guidelines for the diagnosis, staging and treatment of patients with metastatic breast cancer
    Im SA, Gennari A, Park YH, Kim JH, Jiang ZF, Gupta S, et al.
    ESMO Open, 2023 Jun;8(3):101541.
    PMID: 37178669 DOI: 10.1016/j.esmoop.2023.101541
    The most recent version of the European Society for Medical Oncology (ESMO) Clinical Practice Guidelines for the diagnosis, staging and treatment of patients with metastatic breast cancer (MBC) was published in 2021. A special, hybrid guidelines meeting was convened by ESMO and the Korean Society of Medical Oncology (KSMO) in collaboration with nine other Asian national oncology societies in May 2022 in order to adapt the ESMO 2021 guidelines to take into account the differences associated with the treatment of MBC in Asia. These guidelines represent the consensus opinions reached by a panel of Asian experts in the treatment of patients with MBC representing the oncological societies of China (CSCO), India (ISMPO), Indonesia (ISHMO), Japan (JSMO), Korea (KSMO), Malaysia (MOS), the Philippines (PSMO), Singapore (SSO), Taiwan (TOS) and Thailand (TSCO). The voting was based on the best available scientific evidence and was independent of drug access or practice restrictions in the different Asian countries. The latter were discussed when appropriate. The aim of these guidelines is to provide guidance for the harmonisation of the management of patients with MBC across the different regions of Asia, drawing from data provided by global and Asian trials whilst at the same time integrating the differences in genetics, demographics and scientific evidence, together with restricted access to certain therapeutic strategies.
  19. Studies on human filariasis in Malaysia: immunoglobulin and complement levels in persons infected with Brugia malayi and Wuchereria bancrofti
    Joon-Wah M, Singh M, Yap EH, Ho BC, Kang KL
    Trans R Soc Trop Med Hyg, 1979;73(4):395-9.
    PMID: 400204
    Levels of immunoglobulins G, A, M and E as well as complement components C3c and C4 have been determined in populations in various endemic areas in Peninsular Malaysia and also in filariasis patients. High immunoglobulin levels were seen. In the microfilarial-negative group IgG was 2009 mg% while IgE was 3967 I.U./ml. In the filariasis group, Wuchereria bancrofti patients had significantly higher levels of IgG, IgM and IgE, namely, 3314 mg%, 804 mg% and 18400 I.U./ml respectively. The significance of these levels is discussed.
  20. Fulltext Soil-transmitted helminthiasis among children in Pulau Ketam, West Malaysia
    Kan SP, Singh M, Singh S
    Med J Malaysia, 1987 Mar;42(1):40-3.
    PMID: 3431501
    Only 8.4% of 1,286 Chinese boys and girls from the ages of 5-12 years in Pulau Ketam were infected with soil-transmitted helminths. The majority of these children had single infections with Trichuris or Ascaris alone. Mixed infections made up less than 5% of all infected cases. The worm burdens of infected children were very low. There was no definite pattern of distribution of infection among children of different ages and no differences in the distribution of infection between boys and girls. The main factors for the low prevalence and intensity of infection were the absence of suitable soil for the development and survival of infective helminth stages and the lack of contact with contaminated soil on this island. These two factors acted as very effective barriers to the transmission of soiltransmitted helminths on Pulau Ketam.
Related Terms
Filters
Contact Us

Please provide feedback to Administrator (afdal@afpm.org.my)

External Links