Displaying publications 1 - 20 of 435 in total

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  1. Zulkifli FH, Hussain FSJ, Harun WSW, Yusoff MM
    Int J Biol Macromol, 2019 Feb 01;122:562-571.
    PMID: 30365990 DOI: 10.1016/j.ijbiomac.2018.10.156
    This study is focusing to develop a porous biocompatible scaffold using hydroxyethyl cellulose (HEC) and poly (vinyl alcohol) (PVA) with improved cellular adhesion profiles and stability. The combination of HEC and PVA were synthesized using freeze-drying technique and characterized using SEM, ATR-FTIR, TGA, DSC, and UTM. Pore size of HEC/PVA (2-40 μm) scaffolds showed diameter in a range of both pure HEC (2-20 μm) and PVA (14-70 μm). All scaffolds revealed high porosity above 85%. The water uptake of HEC was controlled by PVA cooperation in the polymer matrix. After 7 days, all blended scaffolds showed low degradation rate with the increased of PVA composition. The FTIR and TGA results explicit possible chemical interactions and mass loss of blended scaffolds, respectively. The Tg values of DSC curved in range of HEC and PVA represented the miscibility of HEC/PVA blend polymers. Higher Young's modulus was obtained with the increasing of HEC value. Cell-scaffolds interaction demonstrated that human fibroblast (hFB) cells adhered to polymer matrices with better cell proliferation observed after 7 days of cultivation. These results suggested that biocompatible of HEC/PVA scaffolds fabricated by freeze-drying method might be suitable for skin tissue engineering applications.
  2. Zulfiana D, Karimah A, Anita SH, Masruchin N, Wijaya K, Suryanegara L, et al.
    Int J Biol Macromol, 2020 Dec 01;164:892-901.
    PMID: 32673720 DOI: 10.1016/j.ijbiomac.2020.07.102
    Recently, the interest in active packaging utilization has increased with population growth, food demand and new consumer trend like food delivery services. This new system, however, requires the use of additives to extend the food product quality and safety as well as in maintaining the shelf-life. This study was to prepare the antimicrobial paper from I. cylindrica coated anionic nanocellulose crosslinked cationic to create a system with the ability to actively control microbe growth in the packaging materials. The process involved pulping of I. cylindrica using semi-chemical and soda chemical method. The antimicrobial paper was prepared by printing the pulp suspension in 60 g/m2 grammage in mold followed by the spray of anionic nanocellulose and subsequent soaking of the paper in cationic solution. The results showed the I. cylindrica paper coated anionic nanocellulose crosslinked with H+ and Al3+ cations were successfully produced. The paper produced was also observed to have antimicrobial activity against Gram-negative of E. coli and S. typhi as well as Gram-positive of S. aureus and B. subtilis bacteria. Furthermore, the best coating method was found on antimicrobial paper coated anionic nanocellulose crosslinked Al3+ as evidenced by smoother and compact surface structure.
  3. Zhang Y, Hu M, Zhu K, Wu G, Tan L
    Int J Biol Macromol, 2018 Feb;107(Pt B):1395-1405.
    PMID: 29017887 DOI: 10.1016/j.ijbiomac.2017.10.001
    Jackfruit is now receiving extensive attention as a new source of starch. However, jackfruit seeds are discarded as waste, although they are rich in starch. The functional properties of the starches were investigated from new Chinese jackfruit species. All the starches have a high amylose (26.56-38.34%) with a potential to become functional foods rich in resistant starch. The jackfruit starches varied from trigonal and tetragonal, round to semi-oval/bell shapes and showed significant variations in particle sizes (5.53-14.46μm). These variations led to significant differences in their functional properties, and significant correlations were found in their pasting, thermal, crystal and texture parameters. Hierarchical cluster analysis sorted the samples into three groups of 1) Malaysia 8 (M8) and ZhenZhu (ZZ); 2) Malaysia 2, Malaysia 3 and Malaysia 4, (M2, M3, M4); and 3) Xiangyinsuo 11, Xiangyinsuo 4, Xiangyinsuo 3 and Xiangyinsuo 2 (X11, X4, X3, X2). The first group could be used as food thickening or gelling agents. The second group could be applied in glutinous foods. The third group make them suitable for fillings in confectionery or weaning foods.
  4. Zhang Y, Ren H, Li B, Udin SM, Maarof H, Zhou W, et al.
    Int J Biol Macromol, 2023 Jul 01;242(Pt 2):124829.
    PMID: 37210053 DOI: 10.1016/j.ijbiomac.2023.124829
    Deep eutectic solvents (DESs) composed by amino acids (L-arginine, L-proline, L-alanine) as the hydrogen bond acceptors (HBAs) and carboxylic acids (formic acid, acetic acid, lactic acid, levulinic acid) as hydrogen bond donors (HBDs) were prepared and used for the dissolution of dealkaline lignin (DAL). The mechanism of lignin dissolution in DESs was explored at molecular level by combining the analysis of Kamlet-Taft (K-T) solvatochromic parameters, FTIR spectrum and density functional theory (DFT) calculations of DESs. Firstly, it was found that the formation of new hydrogen bonds between lignin and DESs mainly drove the dissolution of lignin, which were accompanied by the erosion of hydrogen bond networks in both lignin and DESs. The nature of hydrogen bond network within DESs was fundamentally determined by the type and number of functional groups in both HBA and HBD, which affected its ability to form hydrogen bond with lignin. One hydroxyl group and carboxyl group in HBDs provided active protons, which facilitated proton-catalyzed cleavage of β-O-4, thus enhancing the dissolution of DESs. The superfluous functional group resulted in more extensive and stronger hydrogen bond network in the DESs, thus decreasing the lignin dissolving ability. Moreover, it was found that lignin solubility had a closed positive correlation with the subtraction value of α and β (net hydrogen donating ability) of DESs. Among all the investigated DESs, L-alanine/formic acid (1:3) with the strong hydrogen-bond donating ability (acidity), weak hydrogen-bond accepting ability (basicity) and small steric-hindrance effect showed the best lignin dissolving ability (23.99 wt%, 60 °C). On top of that, the value of α and β of L-proline/carboxylic acids DESs showed some positive correlation with the global electrostatic potential (ESP) maxima and minima of the corresponding DESs respectively, indicating the analysis of ESP quantitative distributions of DESs could be an effective tool for DESs screening and design for lignin dissolution as well as other applications.
  5. Zhang J, Mohd Said F, Jing Z
    Int J Biol Macromol, 2023 Dec 31;253(Pt 1):126482.
    PMID: 37640188 DOI: 10.1016/j.ijbiomac.2023.126482
    Chitin is extensively applied in vast applications due to its excellent biological properties, such as biodegradable and non-toxic. About 50 % of waste generated during seafood processing is chitin. Conventionally, chitin is extracted via chemical method. However, it has many shortcomings. Many novel extraction methods have emerged, including enzymatic hydrolysis, microbial fermentation, ultrasonic or microwave-assisted, ionic liquids, and deep eutectic solvents. Chitin and its derivatives-based hydrogels have attracted much attention due to their excellent properties. Nevertheless, they all have many limitations. Therefore, the preparation and application of chitin and its derivatives-based hydrogels are still facing great challenges. This review focuses on the challenges and prospects for sustainable chitin extraction from seafood waste and the preparation and application of chitin and its derivatives-based hydrogels. First section summarizes the mechanism and application of several methods of extracting chitin. The different extraction methods were evaluated from the aspects of yield, degree of acetylation, and protein and mineral residuals. The shortcomings of the extraction methods are also discussed. Next section summarizes the preparation and application of chitin and its derivatives-based hydrogels. Overall, we hope this mini-review can provide a practical reference for selecting chitin extraction methods from seafood and applying chitin and its derivatives-based hydrogels.
  6. Zarudin NH, Normaya E, Shamsuri SS, Iqbal A, Mat Piah MB, Abdullah Z, et al.
    Int J Biol Macromol, 2024 Feb;258(Pt 2):129168.
    PMID: 38171432 DOI: 10.1016/j.ijbiomac.2023.129168
    Tyrosinase is a key enzyme in enzymatic browning, causing quality losses in food through the oxidation process. Thus, the discovery of an effective and natural tyrosinase inhibitor via green technology is of great interest to the global food market due to food security and climate change issues. In this study, Syzygium aqueum (S. aqueum) leaves, which are known to be rich in phenolic compounds (PC), were chosen as a natural source of tyrosinase inhibitor, and the effect of the sustainable, supercritical fluid extraction (SFE) process was evaluated. Response surface methodology-assisted supercritical fluid extraction (RSM-assisted SFE) was utilized to optimize the PCs extracted from S. aqueum. The highest amount of PC was obtained at the optimum conditions (55 °C, 3350 psi, and 70 min). The IC50 (661.815 μg/mL) of the optimized extract was evaluated, and its antioxidant activity (96.8 %) was determined. Gas chromatography-mass spectrometry (GC-MS) results reveal that 2',6'-dihydroxy-4'-methoxychalcone (2,6-D4MC) (82.65 %) was the major PC in S. aqueum. Chemometric analysis indicated that 2,6-D4MC has similar chemical properties to the tyrosinase inhibitor control (kaempferol). The toxicity and physiochemical properties of the novel 2,6-D4MC from S. aqueum revealed that the 2,6-D4MC is safer than kaempferol as predicted via absorption, distribution, metabolism, and excretion (ADME) evaluation. Enzyme kinetic analysis shows that the type of inhibition of the optimized extract is non-competitive inhibition with Km = 1.55 mM and Vmax = 0.017 μM/s. High-performance liquid chromatography (HPLC) analysis shows the effectiveness of S. aqueum as a tyrosinase inhibitor. The mechanistic insight of the tyrosinase inhibition using 2,6-D4MC was successfully calculated using density functional theory (DFT) and molecular docking approaches. The findings could have a significant impact on food security development by devising a sustainable and effective tyrosinase inhibitor from waste by-products that is aligned with the United Nation's SDG 2, zero hunger.
  7. Zaid RM, Mishra P, Tabassum S, Wahid ZA, Sakinah AMM
    Int J Biol Macromol, 2019 Dec 01;141:1147-1157.
    PMID: 31494156 DOI: 10.1016/j.ijbiomac.2019.09.017
    The effect of physicochemical treatment on pectin yield, degree of esterification, along with the kinetics and thermodynamics characteristics was investigated in the present study. Several extraction parameters were observed to have impacted the yield and degree of esterification significantly, and the best extraction condition was as follows: agitation rate of 250 rpm, temperature of 70 °C, extraction time of 120 min, pH 2, and liquid to solid ratio of 10 v/w which has resulted in 28.20% of pectin yield, with DE (degree of esterification) of 57.00%. A theoretical model which describes the extractability, dissolution and degradation rate of pectin to predict the maximal yield at the maximal time was established to study the extraction kinetics of pectin from HPP. The kinetic analysis from Panchev's model shows the extraction rate was found highest at LSR 10 with ymax 30.85%. The calculated activation energy for pectin dissolution and degradation was found to be 4.532 kJ/mol and 28.054 kJ/mol, respectively. The thermodynamic study has suggested that the process was endothermic, spontaneous and reversible. These results suggest that the physical and chemical treatment applied could be an efficient technique for the extraction of pectin from Hylocereus polyrhizus peels.
  8. Zaid RM, Mishra P, Wahid ZA, Sakinah AMM
    Int J Biol Macromol, 2019 Aug 01;134:361-367.
    PMID: 31059740 DOI: 10.1016/j.ijbiomac.2019.03.143
    In the present study, high-methoxyl pectin (HMP) was extracted from Hylocereus polyrhizus peel's using physico-chemical process. In addition, the hypolipidemic activity of HMP was investigated at different concentration and time corresponding to its adsorption ability. FTIR and contact angle analysis were used to determine the sorbent characterization. A high degree of esterification (63.8%) and the contact angle (95.5°) confirmed hydrophobic nature and resulting bad wetting of the HMP extract, respectively. The methoxyl content in the pectin acted as an affinity-precursor of the pectin towards cholesterol due to its increased hydrophobicity. The maximum equilibrium uptake capacity of cholesterol of 370.5mg/g (0.96mmol/g) was observed by HMP. The experimental data showed good fitting for Freundlich isotherm equation and followed pseudo-first-order kinetic model with a correlation coefficient (R2) of 0.89-0.97 due to physisorption mechanism. Intra-particle model confirmed that the cholesterol sorption rate by HMP was significantly influenced by external mass transfer (surface diffusion) and intra-particle diffusion (diffusion control). It was also revealed that the HMP extracted from Hylocereus polyrhizus peels possess a high affinity towards cholesterol, making it an ideal hypolipidemic agent.
  9. Zabidi N', Zainal NN, Tawakkal ISMA, Mohd Basri MS, Ariffin SH, Naim MN
    Int J Biol Macromol, 2023 Aug 09;251:126212.
    PMID: 37567533 DOI: 10.1016/j.ijbiomac.2023.126212
    The present study developed the formulation of active bionanocomposites films endowed with the abilities of high biodegradability and antimicrobials for active packaging applications. The aim of this work was to prepare poly (lactic acid)/poly (butylene succinate) (PLA/PBS) blended films reinforced with different concentrations of nanofibrillated cellulose (NFC) and 9 % of thymol essential oil (EO) using the casting method. The active films were further evaluated through Fourier transform infrared spectroscopy (FTIR); as well as mechanical, physical, water vapour permeability (WVP), thermal analysis (TGA), biodegradation, morphological, and antimicrobial (% reduction of bacteria) testing. The tensile strength (TS) of PLA/PBS blend films increased by 12 % with the incorporation of 2 wt% of NFC. The PLA/PBS/NFC with 9 % thymol EO has a good water barrier performance with its tensile strength, elongation at break, and tensile modulus was 13.2 MPa, 13.1 %, and 513 MPa respectively. The presence of NFC promoted the disintegration of PLA/PBS films by 70.5 %. These films promoted the antibacterial activity against S. aureus and E. coli. The study demonstrates that the developed films improved the qualities of chicken fillets and have great potential to be used as active bionanocomposites in food packaging applications.
  10. Yusuf J, Sapuan SM, Ansari MA, Siddiqui VU, Jamal T, Ilyas RA, et al.
    Int J Biol Macromol, 2024 Jan;255:128121.
    PMID: 37984579 DOI: 10.1016/j.ijbiomac.2023.128121
    Material is an inseparable entity for humans to serve different purposes. However, synthetic polymers represent a major category of anthropogenic pollutants with detrimental impacts on natural ecosystems. This escalating environmental issue is characterized by the accumulation of non-biodegradable plastic materials, which pose serious threats to the health of our planet's ecosystem. Cellulose is becoming a focal point for many researchers due to its high availability. It has been used to serve various purposes. Recent scientific advancements have unveiled innovative prospects for the utilization of nanocellulose within the area of advanced science. This comprehensive review investigates deeply into the field of nanocellulose, explaining the methodologies employed in separating nanocellulose from cellulose. It also explains upon two intricately examined applications that emphasize the pivotal role of nanocellulose in nanocomposites. The initial instance pertains to the automotive sector, encompassing cutting-edge applications in electric vehicle (EV) batteries, while the second exemplifies the use of nanocellulose in the field of biomedical applications like otorhinolaryngology, ophthalmology, and wound dressing. This review aims to provide comprehensive information starting from the definitions, identifying the sources of the nanocellulose and its extraction, and ending with the recent applications in the emerging field such as energy storage and biomedical applications.
  11. Yusof NH, Foo KY, Hameed BH, Hussin MH, Lee HK, Sabar S
    Int J Biol Macromol, 2020 Aug 15;157:648-658.
    PMID: 31790734 DOI: 10.1016/j.ijbiomac.2019.11.218
    Chitosan-polyethyleneimine with calcium chloride as ionic cross-linker (CsPC) was synthesized as a new kind of adsorbent using a simple, green and cost-effective technique. The adsorption properties of the adsorbent for Acid Red 88 (AR88) dye, as a model analyte, were investigated in a batch system as the function of solution pH (pH 3-12), initial AR88 concentration (50-500 mg L-1), contact time (0-24 h), and temperature (30-50 °C). Results showed that the adsorption process obeyed the pseudo-first order kinetic model and the adsorption rate was governed by both intra-particle and liquid-film mechanism. Equilibrium data were well correlated with the Freundlich isotherm model, with the calculated maximum adsorption capacity (qm) of 1000 mg g-1 at 30 °C. The findings underlined CsPC to be an effective and efficient adsorbent, which can be easily synthesized via one-step process with promising prospects for the removal of AR88 or any other similar dyes from the aqueous solutions.
  12. Yusof NAA, Zain NM, Pauzi N
    Int J Biol Macromol, 2019 Mar 01;124:1132-1136.
    PMID: 30496864 DOI: 10.1016/j.ijbiomac.2018.11.228
    Antibacterial activity of zinc oxide (ZnO) nanoparticles have received significant interest, particularly by the implementation of nanotechnology to synthesize particles in nanometer region. ZnO nanoparticles were successfully synthesized through microwave heating by using chitosan as a stabilizing agent and characterized by UV-vis, FTIR, XRD and FESEM-EDX. The aim of the present study is to determine the antibacterial activity of ZnO nanoparticles against Gram-positive bacterium Staphylococcus aureus (S. aureus) and Gram-negative bacterium Escherichia coli (E. coli). The antibacterial effect of ZnO nanoparticles was investigated for the inhibition zone and inactivation of cell growth. The absorption of ZnO nanoparticles was found to be around 360 nm. FTIR results showed the stretching mode of ZnO nanoparticles at 475 cm-1 of the absorption band. EDX results indicated that ZnO nanoparticles have been successfully formed with an atomic percentage of zinc and oxygen at 23.61 and 46.57% respectively. X-ray diffraction result was confirmed the single-phase formation of ZnO nanoparticles and the particle sizes were observed to be around 50 to 130 nm. The results showed that ZnO nanoparticles have displayed inhibition zone of 16 and 13 mm against S. aureus and E. coli respectively. Gram-negative bacteria seemed to be more resistant to ZnO nanoparticles than Gram-positive bacteria.
  13. Yusefi M, Soon ML, Teow SY, Monchouguy EI, Neerooa BNHM, Izadiyan Z, et al.
    Int J Biol Macromol, 2022 Feb 28;199:372-385.
    PMID: 34998882 DOI: 10.1016/j.ijbiomac.2021.12.189
    Polysaccharide nanocrystals have great potential to be used as improved drug carriers due to their low cost, high biodegradability, and biocompatibility. This study reports the synthesis of cellulose nanocrystals (CNC) loaded with 5-fluorouracil (CNC/5FU) to evaluate their anticancer activity against colorectal cancer cells. X-ray and Fourier-transform infrared spectroscopy demonstrated that acid hydrolysis successfully degraded the amorphous cellulose to liberate the crystal regions. From transmission electron microscopy, CNC/5FU appeared as rod-like nanocrystals with an average length and width of 69.53 ± 1.14 nm and 8.13 ± 0.72 nm, respectively. The anticancer drug 5FU showed improved thermal stability after being loading onto CNC. From UV-vis spectroscopy data, the drug encapsulation efficiency in CNC/5FU was estimated to be 83.50 ± 1.52%. The drug release of CNC/5FU was higher at pH 7.4 compared to those at pH 4.2 and 1.2. From the cytotoxicity assays, CNC did not affect the viability of CCD112 colon normal cells. On the other hand, CNC/5FU exhibited anticancer effects against HCT116 and HT-29 colorectal cancer cells. The anticancer actions of CNC/5FU against HCT116 cells were then confirmed using an in vitro tumor-on-chip model and clonogenic assay. Mechanistic studies demonstrated that CNC/5FU killed the cancer cells by mainly inducing cell apoptosis and mitochondrial membrane damage. Overall, this study indicated that CNC/5FU could be a potential nanoformulation for improved drug delivery and colorectal cancer treatment.
  14. Yusefi M, Shameli K, Lee-Kiun MS, Teow SY, Moeini H, Ali RR, et al.
    Int J Biol Macromol, 2023 Apr 01;233:123388.
    PMID: 36706873 DOI: 10.1016/j.ijbiomac.2023.123388
    Polysaccharide-based magnetic nanocomposites can eminently illuminate several attractive features as anticancer drug carriers. In this study, rice straw-based cellulose nanowhisker (CNW) was used as solid support for Fe3O4 nanofillers to synthesize magnetic CNW. Then, cross-linked chitosan-coated magnetic CNW for 5-fluorouracil carrier abbreviated as CH/MCNW/5FU. Fourier-transform infrared, X-Ray diffraction, and X-ray photoelectron spectroscopy analysis indicated successful fabrication and multifunctional properties of the CH/MCNW/5FU nanocomposites. In addition, CH/MCNW/5FU nanocomposites showed hydrodynamic diameter and zeta potential value of 181.31 ± 3.46 nm and +23 ± 1.8 mV, respectively. Based on images of transmission electron microscopy, magnetic CNW as reinforcement was coated with chitosan to obtain almost spherical CH/MCNW/5FU nanocomposites with an average diameter of 37.16 ± 3.08. The nanocomposites indicated desired saturation magnetization and thermal stability, high drug encapsulation efficiency, and pH-dependent swelling and drug release performance. CH/MCNW/5FU nanocomposites showed potent killing effects against colorectal cancer cells in both 2D monolayer and 3D spheroid models. These findings suggest CH/MCNW as a potential carrier for anticancer drugs with high tumour-penetrating capacity.
  15. Yuan Y, Wang YB, Jiang Y, Prasad KN, Yang J, Qu H, et al.
    Int J Biol Macromol, 2016 Jan;82:696-701.
    PMID: 26505952 DOI: 10.1016/j.ijbiomac.2015.10.069
    The water-soluble bioactive polysaccharides can contribute to the health benefits of Lycium barbarium fruit. However, the structure characteristics of these polysaccharides remain unclear yet. An important polysaccharide (LBPA) was isolated and purified from L. barbarium in this work. It was identified by chemical and spectroscopic methods as arabinogalactan with β-d-(1→6)-galactan as backbone, which was different to any reported polysaccharides from this species before. This arabinogalactan was comprised of Araf, Galp, GlcpA and Rhap with a molar ratio of 9.2:6.6:1.0:0.9. The side chains, including α-l-Araf-(1→, α-l-Araf-(1→5)-α-l-Araf-(1→, β-l-Araf-(1→5)-α-l-Araf-(1→ and α-l-Rhap-(1→4)-β-d-GlcpA-(1→6)-β-d-Galp-(1→, were linked to β-d-(1→6)-galactan at O-3. The putative structure was drawn as below. The molecular weight was determined to be 470,000g/mol by gel permeation chromatography.
  16. Yoong WC, Loke CF, Juan JC, Yusoff K, Mohtarrudin N, Tatsuma T, et al.
    Int J Biol Macromol, 2022 Jan 15;201:516-527.
    PMID: 35041888 DOI: 10.1016/j.ijbiomac.2022.01.062
    We report herein the design and synthesis of colloidally-stable S/Ag1.93S nanoparticles, their photothermal conversion properties and in vitro cytotoxicity toward A431 skin cancer cells under the excitation of a minimally-invasive 980 nm near-infrared (NIR) laser. Micron-sized S particles were first synthesized via acidifying Na2S2O3 using biocompatible sodium alginate as a surfactant. In the presence of AgNO3 and under rapid microwave-induced heating, alginate reduced AgNO3 to nascent Ag which reacted with molten S in situ forming S/Ag1.93S nanoparticles. The nanoparticles were characterized using a combination of X-ray diffraction, electron microscopies, elemental analysis, zeta-potential analysis and UV-VIS-NIR spectroscopy. The average particles size was controlled between 40 and 60 nm by fixing the mole ratio of Ag+:S2O32-. When excited by a 980 nm laser, S/Ag1.93S nanoparticles (~40 nm) produced with the least amount of AgNO3 exhibited a respectable photothermal conversion efficiency of circa 62% with the test aqueous solution heated to a hyperthermia-inducing 52 °C in 15 min. At 0.7 W/cm2, the viability of A431 skin cancer cells incubated with 7.0 ± 0.2 μg/mL of S/Ag1.93S nanoparticles reduced to 14 ± 0.6%, while an A431 cell control maintained an 80% cell viability. These results suggested that S/Ag1.93S nanoparticles may have good potential in reducing metastatic skin carcinoma.
  17. Yip YS, Manas NHA, Jaafar NR, Rahman RA, Puspaningsih NNT, Illias RM
    Int J Biol Macromol, 2023 Jul 01;242(Pt 1):124675.
    PMID: 37127056 DOI: 10.1016/j.ijbiomac.2023.124675
    Maltooligosaccharides (MOS) are functional oligosaccharides that can be synthesized through enzymatic cascade reaction between cyclodextrin glucanotransferase (CGTase) and maltogenic amylase (Mag1) from Bacillus lehensis G1. To address the problems of low operational stability and non-reusability of free enzymes, both enzymes were co-immobilized as combined cross-linked enzyme aggregates (Combi-CLEAs-CM) with incorporation of bovine serum albumin (BSA) and Tween 80 (Combi-CLEAs-CM-add). Combi-CLEAs-CM and Combi-CLEAs-CM-add showed activity recoveries of 54.12 % and 69.44 %, respectively after optimization. Combi-CLEAs-CM-add showed higher thermal stability at higher temperatures (40 °C) with longer half-life (46.20 min) as compared to those of free enzymes (36.67 min) and Combi-CLEAs-CM (41.51 min). Both combi-CLEAs also exhibited higher pH stability over pH 5 to pH 9, and displayed excellent reusability with >50 % of initial activity retained after four cycles. The reduction in Km value of about 22.80 % and 1.76-fold increase in starch hydrolysis in comparison to Combi-CLEAs-CM attested the improvement of enzyme-substrate interaction by Tween 80 and pores formation by BSA in Combi-CLEAs-CM-add. The improved product specificity of Combi-CLEAs-CM-add also produced the highest yield of MOS (492 mg/g) after 3 h. Therefore, Combi-CLEAs-CM-add with ease of preparation, excellent reusability and high operational stability is believed to be highly efficacious biocatalyst for MOS production.
  18. Yao J, Li S, Zhang L, Yang Y, Gopinath SCB, Lakshmipriya T, et al.
    Int J Biol Macromol, 2020 May 15;151:1133-1138.
    PMID: 31743722 DOI: 10.1016/j.ijbiomac.2019.10.156
    Haemophilia is a blood clotting disorder known as 'Christmas disease' caused when the blood has defect with the clotting factor(s). Bleeding leads various issues, such as chronic pain, arthritis and a serious complication during the surgery. Identifying this disease is mandatory to take the necessary treatment and maintains the normal clotting. It has been proved that the level of factor IX (FIX) is lesser with haemophilia patient and the attempt here is focused to quantify FIX level by interdigitated electrode (IDE) sensor. Single-walled carbon nanotube (SWCNT) was utilized to modify IDE sensing surface. On this surface, dual probing was evaluated with aptamer and antibody to bring the possible advantages. The detection limit with antibody was found to be 1 pM, while aptamer shows 100 fM. Further, a fine-tuning was attempted with sandwich pattern of aptamer-FIX-antibody and antibody-FIX-aptamer and compared. Specific elevation of detection with 10 folds was noticed and displayed the detection at 100 f. in both sandwich patterns. In addition, FIX was detected in the diluted human serum by aptamer-FIX-antibody sandwich, it was found that FIX detected from the dilution factor 1:640. A novel demonstration is with higher discrimination against other clotting factors, XI and VII.
  19. Yang Y, Aghbashlo M, Gupta VK, Amiri H, Pan J, Tabatabaei M, et al.
    Int J Biol Macromol, 2023 May 01;236:123954.
    PMID: 36898453 DOI: 10.1016/j.ijbiomac.2023.123954
    Large amounts of agricultural waste, especially marine product waste, are produced annually. These wastes can be used to produce compounds with high-added value. Chitosan is one such valuable product that can be obtained from crustacean wastes. Various biological activities of chitosan and its derivatives, especially antimicrobial, antioxidant, and anticancer properties, have been confirmed by many studies. The unique characteristics of chitosan, especially chitosan nanocarriers, have led to the expansion of using chitosan in various sectors, especially in biomedical sciences and food industries. On the other hand, essential oils, known as volatile and aromatic compounds of plants, have attracted the attention of researchers in recent years. Like chitosan, essential oils have various biological activities, including antimicrobial, antioxidant, and anticancer. In recent years, one of the ways to improve the biological properties of chitosan is to use essential oils encapsulated in chitosan nanocarriers. Among the various biological activities of chitosan nanocarriers containing essential oils, most studies conducted in recent years have been in the field of antimicrobial activity. It was documented that the antimicrobial activity was increased by reducing the size of chitosan particles in the nanoscale. In addition, the antimicrobial activity was intensified when essential oils were in the structure of chitosan nanoparticles. Essential oils can increase the antimicrobial activity of chitosan nanoparticles with synergistic effects. Using essential oils in the structure of chitosan nanocarriers can also improve the other biological properties (antioxidant and anticancer activities) of chitosan and increase the application fields of chitosan. Of course, using essential oils in chitosan nanocarriers for commercial use requires more studies, including stability during storage and effectiveness in real environments. This review aims to overview recent studies on the biological effects of essential oils encapsulated in chitosan nanocarriers, with notes on their biological mechanisms.
  20. Yang Y, Gupta VK, Du Y, Aghbashlo M, Show PL, Pan J, et al.
    Int J Biol Macromol, 2023 Jul 01;242(Pt 2):124800.
    PMID: 37178880 DOI: 10.1016/j.ijbiomac.2023.124800
    Mucilages are natural compounds consisting mainly of polysaccharides with complex chemical structures. Mucilages also contain uronic acids, proteins, lipids, and bioactive compounds. Because of their unique properties, mucilages are used in various industries, including food, cosmetics, and pharmaceuticals. Typically, commercial gums are composed only of polysaccharides, which increase their hydrophilicity and surface tension, reducing their emulsifying ability. As a result of the presence of proteins in combination with polysaccharides, mucilages possess unique emulsifying properties due to their ability to reduce surface tension. In recent years, various studies have been conducted on using mucilages as emulsifiers in classical and Pickering emulsions because of their unique emulsifying feature. Studies have shown that some mucilages, such as yellow mustard, mutamba, and flaxseed mucilages, have a higher emulsifying capacity than commercial gums. A synergistic effect has also been shown in some mucilages, such as Dioscorea opposita mucilage when combined with commercial gums. This review article investigates whether mucilages can be used as emulsifiers and what factors affect their emulsifying properties. A discussion of the challenges and prospects of using mucilages as emulsifiers is also presented in this review.
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