Displaying publications 1 - 20 of 341 in total

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  1. Febrianingtyas, Yunita, Februhartanty, Judhiastuty, Hadihardjono, Dian Nurcahyati
    Malays J Nutr, 2019;25(1):129-142.
    MyJurnal
    Introduction: The primary reason that compels working mothers to decrease their
    ability to continue to breastfeeding successfully is their return to work. Attempts
    to continue breastfeeding at work encounters several difficulties. This study
    aims to provide an overview of the workplace environment and how facilities at
    the workplace affect breastfeeding in Indonesia. Methods: We used a qualitative
    approach to provide a detailed picture of the influence of workplace support for
    working mothers to perform breastfeeding. We enrolled working mothers (n=18)
    in Jakarta, as participants and obtained the following information from them:
    parity, type of family and type of work. Results: The findings provided the general
    overview of the workplace environment and identified three factors that affected
    breastfeeding: seeking information during pregnancy, expression of breast milk at
    office facilities and the problem faced by working mothers. Conclusion: Although
    the breastfeeding performance of most working mothers in this study comprised
    exclusive breastfeeding, some fed their infants with formula milk. This study
    suggests that working mothers who continue to breastfeed after returning to work
    need the support of their employers, co-workers and others in the workplace to
    ensure the provision of health facilities and the protection and dissemination the
    rights of breastfeeding working mothers that might lead them to discontinue their
    breastfeeding practice.
  2. Koo, H.C., S, Daniel Robert, Hamid Jan
    Malays J Nutr, 2013;19(2):163-172.
    MyJurnal
    Introduction: The increasing prevalence of adiposity is associated with the development of type 2 diabetes mellitus (T2DM). The two most popular methods to measure adiposity are body mass index (BMI) and waist-hip-ratio (WHR), which measure the overall and abdominal adiposity, respectively. The aim of this study was to determine whether BMI or WHR was more strongly associated with macronutrient intakes and lipid profiles in T2DM patients. Methods: In this cross-sectional study, a total of 210 T2DM patients, mean age of 56.73(SD 10.23) years, were recruited from Penang. Information on socio-demographic, body measurements (height, weight, waist circumference and hip circumference)and macronutrient intakes (three 24-hour dietary recalls) were recorded. Routine laboratory tests (fasting blood glucose, HbA1c, LDL, HDL, triglyceride and total cholesterol) were performed according to standard procedure. Results:Among the respondents, 84% had abdominal and 63% had overall adiposity respectively.There was a significant correlation between WHR and the total calorie(r=0.27; p
  3. Chee YF, Roseline Yap WK, Siti Sabariah B
    Malays J Nutr, 2008 Mar;14(1):79-89.
    PMID: 22691766 MyJurnal
    A cross-sectional study was conducted among 13 female children and 40 adolescents residing in a welfare home. The objectives of this study were to determine underweight, overweight rates and body fat percentages as well as assess the dietary intake of energy, selected macro- and micro-nutrients among the subjects aged 6-17 years. The anthropometric measurements collected were Body Mass Index (BMI) and skinfold thickness of five body sites - triceps, subscapular, abdomen, suprailiac, and thigh. Dietary intake was assessed using a 24-hour dietary recall. This study indicated that the majority (75%) of the subjects were of normal weight, 21% were underweight and 4% were overweight. BMI and body fat percentages of the adolescents were found to be significantly higher than those of the children (t=-3.083, p=0.003; t=-7.321, p<0.001). A positive correlation between BMI and body fat percentage was also significant (r=0.791, p<0.001). In terms of dietary assessment, there were significant differences in percentage of RNI attainment between children and adolescents for energy, protein, iron, and folate (p<0.05). In conclusion, the majority of the children and adolescents had normal weight and average body fat percentages. Besides, all the children met the RNI requirements for all the studied nutrients while the adolescents met the RNI requirements for energy, protein, iron, and zinc with dietary calcium and folate slightly below the RNI requirements.
  4. Hashim N, Pongjata J
    Malays J Nutr, 2000 Mar;6(1):65-73.
    PMID: 22692393
    The objective of this study was to identify the effect of different drying methods on vitamin A activity of formulated weaning food. Weaned foods on vitamin A activity of formulated using treated cowpea flour, locally available rice flour, banana-pumpkin, skim milk powder and sugar in the ratio 35:35:15:15:5. Treated cowpea flour consisted of original cowpea flour, 24 h germinated cowpea flour. Each treated flour was mixed separately with the other ingredients and cooked into a slurry. Each mixture was either oven-dried or freeze-dried to produce a dry flaky mixture. The carotenoid composition of the product was determined by HPLC. Vitamin A activity of oven-dried weaning food was significantly reduced (p<0.05) compared to freeze-dried weaning food. The freeze-dried weaning foods showed a higher retinol equivalent than oven-dried weaning foods for all treatments. The results of the study found that an intake of 100 g of freeze-dried weaning foods enriched with banana-pumpkin and cowpea flour provided an adequate amount of the recommended daily allowance (RDA) of vitamin A for infants.
  5. Gan WY, Mohd NM, Siti AH, Zalilah MS
    Malays J Nutr, 2012 Dec;18(3):295-306.
    PMID: 24568070 MyJurnal
    This study aimed to evaluate the validity and reliability of the Multidimensional Body Image Scale (MBIS), a seven-factor, 62-item scale developed for Malaysian female adolescents. This scale was evaluated among male and female Malaysian university students.
  6. Wan Fadzlina Wan Muhd Shukeri, Samiullah Saeed, Azrina Md Ralib, Mohd Basri Mat-Nor
    Malays J Nutr, 2019;25(4):413-421.
    MyJurnal
    Introduction: The mNUTRIC score is a nutritional assessment tool to identify critically
    ill patients with high nutritional risk who could benefit from nutritional interventions.
    This study was conducted to validate the 28-day mortality prognostic performance
    of the mNUTRIC score in a Malaysian intensive care unit (ICU). Methods: This was
    a retrospective cohort study of adult patients who were consecutively admitted to
    the ICU from January 2017 to December 2018 for >24 hours. Data were collected on
    variables required to calculate the mNUTRIC score. Patients with mNUTRIC score
    ≥5 points were considered to be at high nutritional risk. Main outcome was 28-
    day mortality from all causes; ICU length of stay (LOS) and prolonged mechanical
    ventilation (MV) (>2 days) were secondary outcomes. Results: From a total of 432
    admissions, 382 (88.4%) patients fulfilled the study criteria. Seventy-seven (20.2%)
    of these patients were at high nutritional risk. They had longer mean ICU LOS
    (7.1±7.5 days versus 4.2±4.0 days, p=0.001), greater proportion of prolonged MV
    (57.1% versus 14.4%, p
  7. Suzana S, Siti Saifa H
    Malays J Nutr, 2007 Mar;13(1):29-44.
    PMID: 22692187 MyJurnal
    This cross sectional study was conducted to determine the validity of three screening tools, Mini Nutritional Assessment Short Form (MNA-SF), Malnutrition Risk Screening Tool for Community (MRST-C) and Malnutrition Risk Screening Tool for Hospital (MRST-H) among elderly people at health clinics. The screening tools were validated against anthropometric and functional assessments. The anthropometric assessments that were carried out included body weight, height, arm span, body mass index (BMI), calf circumference (CC) and mid upper arm circumference (MUAC). A set of questionnaire on manual dexterity, muscular strength, instrumental activities daily living (IADL) and cognitive status was used to assess functional abilities. A total of 156 subjects were recruited from rural (38 subjects) and urban (118 subjects) health clinics at Sabak Bernam and Cheras respectively. Subjects' age ranged from 60 to 83 years old, with 44.2% were men and 55.8% women. The prevalence of muscle wasting among the subjects assessed from MUAC and CC were both 7.0%. MNA-SF had the highest correlation with BMI (r = 0.497, p<0.001), followed by MUAC (r = 0.398, p<0.001), CC (r = 0.473, p<0.001), cognitive assessment (r = 0.229, p<0.001) and handgrip strength (r = 0.209, p<0.001). Whilst MRST-C had the highest correlation with IADL score (r =-0.320, p<0.001) and MRST-H had the highest correlation with the lock and key test (r = -0.325, p<0.01). Sensitivity was the highest for MNA-SF (93.2%), followed by MRST-H (52.5%) and MRST-C (25.8%). Specificity was the highest for MRST-H (97.3%), followed by MRST-C (90.8%) and MNA-SF (79.4%). Positive predictive value (PPV) for MRST-H, MNA-SF and MRST-C was 55.5%, 18.2% and 14.1%, respectively. In conclusion, among the screening tools being validated, MNA-SF is considered the most appropriate tool to be used in health clinics for identification of elderly individuals who are at high risk of malnutrition.
  8. Nurhidayah, M., Suzana, S., Zahara, A.M., Mahadir, A., Mohd Azahadi, O., Mohamad Hasnan, A., et al.
    Malays J Nutr, 2016;22(2):233-243.
    MyJurnal
    Introduction: Omega-3 Polyunsaturated Fatty Acids (PUFAs) play an important role in developing cognitive function in children, especially Docosahexanoic Acid (DHA). However, there is no suitable dietary assessment tool to assess DHA intake among Malaysian children. Thus, this study assessed the validity of an interviewer-administered semi-quantitative Food Frequency Questionnaire (FFQ) for estimating DHA intake among Malay school children in the Sepang District.

    Methods: Thirty 12-year-old Malay children (13 boys and 17 girls) were recruited through purposive sampling. Their DHA intake for one month duration was assessed using a 30-food item FFQ and validated against a 3-day food record.

    Results: The majority of the subjects (70%) had normal body mass index, 6.7% were overweight, 16.7% obese, whilst the rest were in the thin category. The reliability of FFQ was found to be good with a Cronbach’s α coefficient value of 0.815. Wilcoxon Signed Rank Test indicated no significant difference in mean intake between the two assessment methods. Significant and strong correlation between FFQ and the 3-day food record was found for total omega-3 PUFAs (r=0.812)and DHA (r=0.839) using both methods. BlandAltman analysis exhibited no apparent systematic bias between the two methods for DHA and total omega-3 PUFAs intake, whilst a quartile analysis assigned 73.3% of the subjects into the same quartile.

    Conclusion: The FFQ was found to be valid in estimating DHA intake among Malay school children, and it is recommended that its validity be tested on other ethnic population groups.
  9. Shahar S, Earland J, Abdulrahman S
    Malays J Nutr, 2000 Mar;6(1):33-44.
    PMID: 22692390 MyJurnal
    Energy and nutrient intake estimated using a pre-coded dietary history questionnaire (DHQ) was compared with results obtained from a 7-d weighed intake record (WI) in a group of 37 elderly Malays residing in rural areas of Mersing District, Johor, Malaysia to determine the validity of the DHQ. The DHQ consists of a pre-coded dietary history with a qualitative food frequency questionnaire which was developed to obtain information on food intake and usual dietary habits. The 7-d WI requires subjects to weigh each food immediately before eating and to weigh any leftovers. The medians of intake from the two methods were rather similar and varied by less than 30% for every nutrient, except for vitamin C (114%). For most of the nutrients, analysis of group means using the Wilcoxon matched pairs signed rank sum test showed no significant difference between the estimation of intake from the DHQ and from the WI, with the exceptions of vitamin C and niacin. The DHQ significantly overestimated the intake of vitamin C compared to the WI (p<0.05), whilst, the intake of niacin was significantly underestimated (p<0.05). The consistency of ranking as assessed using the Spearman's rank correlation coefficient (r) was satisfactory since there were positive correlations between all of the investigated nutrients estimated using the DHQ with those assessed using the WI, except for niacin. Furthermore, both the DHQ and the WI classified approximately 38 to 62% of the subjects into the same tertile for all nutrients, except vitamin C. Therefore, the DHQ was modified by adding a checklist of foods rich in vitamin C and niacin. In conclusion, the DHQ was fairly valid for obtaining the usual intake of most nutrients, particularly on a group basis. These findings indicate that in an elderly population with a high prevalence of illiteracy, a specially designed DHQ can provide very similar estimations to that obtained from 7-d WI.
  10. Satvinder Kaur, Hip, Seng Yim, Hamid Jan Jan Mohamed, Rohana Abdul Jalil, Barakatun-Nisak Mohd. Yusof
    Malays J Nutr, 2016;22(2):245-255.
    MyJurnal
    Introduction: Validation of a culturally specific FFQ is important in assessing habitual dietary intake of ethnic population groups. This study aimed to assess the validity and reproducibility of a FFQ developed specifically for determining the dietary intake of Malaysian Punjabis.

    Methods: Subjects were approached through voluntary participation for the development and validation phase in the Klang Valley. A list of foods consumed by participants (n=100) was documented through a 3-day dietary recall. The validation process was conducted by verifying the developed FFQ against another group of Punjabi adult volunteers (n=101) who kept a 2-day dietary record. Macronutrients, dietary fibre, fatty acids, cholesterol, three types of minerals (calcium, sodium, iron) and four vitamins (B12, folate, C, A) were included in the analysis. Reproducibility was shown with intraclass correlation (ICC) values between FFQ1 vs FFQ2 that were administered 6 months apart among 32 participants.

    Results: In the validation study, the FFQ1 was found to have over-estimated almost all nutrients compared to those in the dietary records. The Spearman correlation coefficients for energy, carbohydrate, protein and fat intake based on the FFQ1 and 2-day dietary records were 0.54, 0.38, 0.47, and 0.31, respectively. The classification into the same and adjacent quartiles was between 61- 84% for the nutrients consumed. Bland Altman plots showed relatively good agreement (between ±2 standard deviation) for both the dietary methods used. Reproducibility analysis of ICC (FFQ1 vs FFQ2) was between 0.46-0.76 for macronutrients and 0.20-0.92 for micronutrients.

    Conclusion: The developed FFQ could be used as a valid tool for assessing dietary intake of Malaysian Punjabis, as it showed a moderate agreement with dietary record for intake of energy and macronutrients.
  11. Ng TK, Low CX, Kong JP, Cho YL
    Malays J Nutr, 2012 Dec;18(3):393-7.
    PMID: 24568080 MyJurnal
    Carotenoid-rich red palm oil (RPO)-based snacks have been provided to children in impoverished communities to improve their vitamin A status. The non-availabilty of information on the acceptability of RPO-based snacks by Malaysian aborigines (Orang Asli) children forms the basis of this study.
  12. Loke, Mi Mi, Chong, Kah Hui, Noor Salihah Zakaria, Hayati Mohd Yusof
    Malays J Nutr, 2017;23(2):227-238.
    MyJurnal
    Introduction: Chinese Herbal Medicine (CHM) is becoming increasingly popular among cancer patients worldwide. While health-related quality of life (HRQoL) in relation to cancer outcomes has attracted global attention, there are few studies on CHM use and HRQoL among cancer patients in Malaysia. This study attempted to determine the association between use of CHM, including the types, reasons, and beliefs, and HRQoL among Malaysian cancer patients.
    Methods: A cross-sectional study was conducted among 120 cancer patients (60 male and 60 female) recruited from government oncology clinics in Johor state. A purposive non-probability sampling was applied to recruit respondents.
    Results: Use of CHM was reported by about half of the patients (49.2%). Common types of CHM used included Chinese herbal extracts (27.5%), Sabah snake grass (12.2%), and ginger (11.5%). The median score for overall belief in CHM significantly differed between
    CHM users (71.7%) and non-users (65.0%) (p<0.001). The HRQoL was generally reported as ‘good’ with high scores for overall functioning scales and low scores for symptom scales. However, no significant difference in HRQoL between CHM users and non-users was noted. ‘Social Functioning’ scores between both groups showed a significant difference (p < 0.001) with respect to sex, with females scoring higher than males. No significant association was noted between CHM use and socio-demographic characteristics except for sex.
    Conclusion: There is a high prevalence of self-prescription of CHM among the cancer patients studied. Nonetheless use of CHM did not show any significant difference in terms of quality of life among CHM users.
    Study site: Oncology clinics, Hospital Sultan Ismail (Johor Bahru) and Hospital Sultanah Nora Ismail (Batu Pahat), Johor, Malaysia
  13. Cheong, S.M., Jasvindar Kaur, Lim, K.H., Ho, B.K., Mohmad, S.
    Malays J Nutr, 2013;19(3):353-362.
    MyJurnal
    Introduction: Consumers are advised to read the nutrition labeling when purchasing packaged food. To what extent consumers read nutrition labeling and understand what they read is not well established among Malaysian older persons. Methods: Data from the National Health and Morbidity Survey III (NHMS III) undertaken in 2006 was analysed to determine the use and understanding of nutrition labeling and its associated factors among free living elderly men and women aged ~60 years. Descriptive analysis and binary Logistic Regression were used to analyse the data. Results: A total of 4,898 respondents provided self-reported information on their use and understanding of nutrition labeling when they bought or received food. Use of nutrition labeling was higher among elderly men [61.9% (95% CI: 59.6-64.1)] than for women [36.6% (95% CI: 34.5-38.8)]. Nutrition labeling use was significantly associated with age, formal education, higher household income levels and marital status among both elderly men and women. Understanding of nutrition labeling among elderly men and women was 91.8% (95% CI: 90.1-93.2) and 89.7% (95% CI: 87.4-91.7) respectively and was significantly associated with formal education for both elderly men and women. Conclusion: Overall, the reading of nutrition labeling among Malaysian elderly is moderate. Elderly men and women with formal education were more likely to understand nutrition labeling. The importance of reading nutrition labels should be inculcated in consumers including older persons so that they choose foods that are nutritious and safe.
    Study name: National Health and Morbidity Survey (NHMS-2006)
  14. Chia, Yoke Yin, Ton, So Ha
    Malays J Nutr, 2006;12(1):67-78.
    MyJurnal
    The objective of the study was to quantify and to profile the amino acids content in urine samples. The amino acids content in urine was determined in 162 individuals (62 young non-vegetarians aged 15-45 years, 24 elderly non-vegetarians aged 46-70 years, 40 young vegetarians and 36 elderly vegetarians) by high performance liquid chromatography (HPLC). The most common amino acids detected in the young and elderly individuals on vegetarian and non-vegetarian diets were phenylalanine, threonine, arginine and asparagine, while leucine, aspartic acid and alanine were not found in any urine samples in both groups. Isoleucine was not detected in the urine of vegetarians. The concentrations of the majority of essential amino acids were between 0.10 - 2.00 mgl24hrs except for histidine which had a range of 4.1 - 5.0 mgl24hrs. The concentrations of non-essential amino acids varied. Proline, glycine and tyrosine concentrations were between 0.10 - 1.00 mg/24hrs, while cysteine, glutamine, glutamic acid and cystine concentrations were between 11.0 - 21.0 mg124hrs. Asparagine and hydroxy-proline had a range of 0.10 - 5.00 mg/24hrs, while serine and arginine ranged between 31.0 - 50.0 mg124hrs. Isoleucine and serine were not detected in elderly vegetarians while histidine, glycine, glutamic acid and hydroxy-proline were not detected in elderly non-vegetarians. Isoleucine, glycine and hydroxy proline were detected in young non-vegetarians but not in young vegetarians. The levels of amino acids showed no significant statistical differences between young vegetarians and non-vegetarians as well as between elderly vegetarians and non-vegetarians. Phenylalanine, threonine and trypthophan were commonly detected in the lacto-ovo and lacto vegetarians, while valine, cysteine, arginine and asparagine were commonly detected in vegans. In conclusion, except for isoleucine, general differences were seen in urinary amino acid excretions between vegetarians and non-vegetarians even though the differences were statistically not significant. Therefore lacto-ovo diets could be nutritionally adequate as the nutrients were substituted by dairy or plant products.
  15. Ahmad Ali Zainuddin, Norazmir Md. Nor, Safiah Md Yusof, Adriana Irawati Nur Ibrahim, Tahir Aris, Foo, Leng Huat
    Malays J Nutr, 2019;25(3):261-271.
    MyJurnal
    Introduction: Under-reporting of energy intake is a common cause of bias
    in nutritional studies. This study was aimed at examining the extent of underreporting of energy intake and its related characteristics among respondents in
    the Malaysian Adult Nutrition Survey (MANS) 2003 and MANS 2014. Methods:
    The present study analysed energy intakes of 9,624 adults aged 18-59 years from
    the MANS in year 2014 (2,890 respondents) and 2003 (6,734 respondents) using
    a single 24-hour diet recall. Basal metabolic rates (BMR) were calculated from the
    age- and gender-specific equations of Schofield. Under-reporting was defined as an
    energy intake:BMR ratio of
  16. Al-Shookri A, Khor GL, Chan YM, Loke SC, Al-Maskari M
    Malays J Nutr, 2011 Apr;17(1):129-41.
    PMID: 22135872 MyJurnal
    During the past four decades, Oman has undergone a rapid socioe-conomic and epidemiological transition leading to a substantial reduction in the prevalence of various communicable diseases, including vaccine-preventable diseases. Health care planning together with the commitment of policy makers has been a critical factor in this reduction. However, with rapid social and economic growth, lifestyle-related non communicable diseases have emerged as new health challenges to the country. Diabetes and obesity are leading risks posed by the chronic diseases. The burden of diabetes has increased sharply in Oman over the last decade, rising from 8.3% in 1991 to 11.6% in 2000 among adults aged 20 years and older. The World Health Organization (WHO) predicted an increase of 190% in the number of subjects living with diabetes in Oman over the next 20 years, rising from 75,000 in 2000 to 217,000 in 2025. There is a lack of awareness of the major risk factors for diabetes mellitus in the Omani population generally. As education is often the most significant predictor of knowledge regarding risk factors, complications and the prevention of diabetes, health promotion in Oman is deemed critical, along with other prevention and control measures. Suitable prevention strategies for reducing the prevalence of diabetes in Oman are discussed. Recommendations are made for reforms in the current health care system; otherwise, diabetes will constitute a major drain on Oman's human and financial resources, threatening the advances in health and longevity achieved over the past decades.
  17. Malays J Nutr, 1995;1(1):-.
    MyJurnal
    An examination of the fat composition of the diet of a Malaysian urban hostel population obtained by chemical analysis of representative meals prepared by a 7-day rotation menu, revealed both nutritional attributes and limitations when compared against the dietary messages contained in the American Heart Association (AHA) and World Health Organisation (WHO) models. The Malaysian diet supplies 26% kcal i.e. 66 g total fat (51 g vegetable fats, 15 g animal fats) and contains
  18. Hazirah, A.R., Abdah, M.A., Zainal, B.
    Malays J Nutr, 2013;19(2):223-232.
    MyJurnal
    Introduction: Cancer chemopreventive agents from natural sources have been actively investigated over the years to seek prevention against cancer. In this study, cocoa polyphenols extract (CPE) was examined to explore its antioxidant and cytotoxicity activities. Methods: CPE was analysed for total phenolic content (TPC) and antioxidant activity (DPPH radical scavenging activity and FRAP ferric-reducing antioxidant power assays). In vitro cytotoxicity effect of CPE
    against HepG2, HT-29, HeLa, MCF-7, MDA-MB-231 and WRL-68 cell lines after 48 h exposure was measured by MTT assay. Results: The study showed that CPE had higher total phenolic content (13560.0±420.1 mg GAE/100g dry weight of sample) than vitamin E (p
  19. Mohd SM, Abdul Manan MJ
    Malays J Nutr, 2012 Apr;18(1):125-36.
    PMID: 23713236 MyJurnal
    The haruan (Channa striatus) is an indigenous, predatory freshwater fish of Malaysia. It is a common food fish among the local populace with traditionally identified pharmacological benefits in treating wound and pain and in boosting energy of the sick. Channa striatus is also a subject of renewed interest in Malaysian folk medicine in the search for a better cure for diseases and ailments. Amino acids and fatty acids, found in high concentrations in the fish, might have contributed to its pharmacological properties. Important amino acids of the fish include glycine, lysine and arginine, while its fatty acids are arachidonic acid, palmitic acid and docosahexaenoic acid. They appear to effect their influence through the formation of several types of bioactive molecules. Extracts of the fish are produced from whole fish, roe, mucus and skin of the fish. This review updates research findings on potential uses of Channa striatus, beyond the traditional prescription as a wound healer, pain reliever and energy booster to include its properties as a ACE-inhibitor, anti-depressant and neuroregenerative agent. The fish appears to have wide-ranging medical uses and should be studied more intensively to unearth its other properties and mechanisms of action.
  20. Maznah I
    Malays J Nutr, 1999 Dec;5(1):31-45.
    PMID: 22692356
    Iron absorption is essential for the maintenance of iron levels in the body, since excretion is poorly regulated. Dietary factors can influence iron absorption including low molecular weight substances such as ascorbic acid which has been shown to enhance iron transport across mucosal cell monolayers. Both in vivo and in vitro work may be carried out to study iron absorption. Studies in vivo have the drawback of dealing with a complex system in which it is difficult to determine the relative importance of different factors. In vitro cell culture models could overcome this difficulty but attempts to establish differentiated enterocyte cell lines in culture have not been successful. However the Caco-2 line, derived from a colon carcinoma, is able to differentiate spontaneously when grown in standard culture conditions. The differentiated cells polarized, formed microvilli and T-junctions associated with the duodenal enterocytes brush border. This cell line thus represents an appropriate model for the study of transport mechanisms related to the intestinal barrier and can be used to study the absorption of nutrients especially iron in relation to dietary intake in particular pertaining to dietary factors that may affect absorption. In this work we have therefore used differentiated Caco-2 cells grown in bicameral chambers as a intestinal cell model to study the absorption of iron from different sources and compared it with INT 407 cells. Transfer of iron across the monolayers in the apical-to-basolateral direction has been found to be greater from feric lactoferrin than from iron citrate, while very little transport occurred from Fe-transferrin. It is concluded that in this in vitro study lactoferrin but not transferrin enhances mucosal iron transport. More importanty this study has also shown that Caco-2 can be used as an in vitro method to investigate not only iron bioavailability but can be applied to other minerals as well.
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