Displaying publications 1 - 20 of 399 in total

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  1. Nazrun, A.S., Aqilah-Sn, S.M.Z, Sabarul, A.M., Isa, N.M., Zulfarina, S.M.
    Medicine & Health, 2014;9(1):1-10.
    MyJurnal
    Osteoporosis dikenali sebagai penyakit senyap kerana tidak mempunyai tanda-tanda awal. Ini disebabkan oleh kepadatan tulang yang berkurangan secara perlahanlahan seiring dengan peningkatan usia. Insiden penyakit ini semakin meningkat setiap tahun di seluruh dunia. Mengukur ketumpatan mineral tulang (BMD) menggunakan densitometry tulang konvensional (DXA) adalah praktikal dalam diagnosis osteoporosis tetapi kosnya adalah tinggi dan tidak dapat dilaksanakan dalam masyarakat. Untuk mengukur ketumpatan tulang, “quantitative ultrasound” (QUS) adalah teknik yang agak moden untuk diagnosis osteoporosis. Ianya agak mudah, konsisten, lebih murah dan kaedah yang selamat berbanding dengan teknik densitometry yang lain. Kedua-dua parameter QUS yang diukur pada masa kini adalah ultrasound jalur pengecilan (BUA) dan kelajuan bunyi (SOS). QUS juga dapat menjangka risiko patah. Ianya kini digunakan untuk memantau tindakbalas kepada rawatan anti-osteoporosis. Kajian in-vitro menunjukkan bahawa indeks QUS berhubungkait dengan BMD, bentuk tulang mikro dan parameter mekanikal. Oleh yang demikian, QUS berupaya untuk menjadi teknik baru untuk penilaian tulang.
  2. Nor Rizan, K., Abdul Manaf ,A., Sabariah, A.R., Siti Aishah, M.A., Noorjahan Banu, M.A., Zubaidah, Z.
    Medicine & Health, 2011;6(1):59-67.
    MyJurnal
    Human papillomavirus (HPV) plays an important role in the pathogenesis of cervical cancer. HPV has been found in 99.7% of cervical cancers worldwide. In Malaysia, it is the second most common cancer among women in all major ethnic groups. The main purpose of this study was to establish the method of SyBrGreen Real-Time PCR and apply it for identification of multiple infections of the two high risk HPV subtypes. In this study, 57 positive samples for HPV 16 and HPV 18 were used to establish a simple
    and sensitive method to detect and identify HPV infection in the cervical neoplasia at different stages of the disease by using real-time ABICycler SyBrGreen 1 technology. The results showed 67 HPV genomes in 57 samples. HPV 16 genome was detected in 55/67 (82%) cases while HPV 18 was detected in 8/67 (12%) cases with 4 cases showing multiple infections of HPV 16 and HPV 18. HPV 16 was the most prevalent followed by HPV 18. Using SyBr Green Real-Time PCR techniques, the results
    showed that DNA melting curve for HPV 16 had a peak around 80.2ºC and Ct value of 20 cycles whereas the DNA melting curve for HPV 18 around 79.2ºC and Ct value of 22 cycles. In conclusion, a SyBr Green Real-Time PCR method has the potential for clinical usage in detection and identification of HPV infection in cervical neoplasia at different stages of the disease.
  3. Irmi Elfina, R., Ezalia, E., Elizabeth, G., Wan Hayati, M.Y, Norhanim, A., Wahidah, A., et al.
    Medicine & Health, 2014;9(1):44-52.
    MyJurnal
    Thalassaemia screening programme has been conducted in Malaysia since 2004. The aim of the programme was to reduce the burden of the disease by identifying thalassaemia carriers. However, the response towards the screening activities was unsatisfactory as there was lack of public awareness against the importance of thalassaemia screening. An alternative approach is to screen blood donors. The purpose of this study was to observe the prevalence of thalassaemia carriers among healthy blood donors. Seven hundred and thirty eight healthy blood donors were screened in Hospital Tengku Ampuan Rahimah, Klang from July to September 2010 using cation-exchange high performance liquid chromatography (HPLC). Cases with haemoglobin variants were further analyzed by gel electrophoresis at alkaline pH. Result shows that the blood donors consisted of 413 Malays (56%), 162 Indians (22%), 148 Chinese (20%) and 15 others (2%). There were 19 (2.6%) individuals with haemoglobin E trait, six (0.8%) with co-inheritance of haemoglobin E and αα- thalassaemia and five (0.7%) with β-thalassaemia trait. Haemoglobin Constant Spring and haemoglobin A2 prime were observed in two (0.3%); and Haemoglobin Lepore and alpha chain variant in one (0.2%). αα-thalassaemia and normal haemoglobin A2 β-thalassaemia could not be excluded in 190 cases (26%), as they required deoxyribonucleic acid (DNA) studies for identification. Thalassaemia screening in blood donors is more feasible and effective. Therefore, a wider scale population screening including blood donors could benefit the existing thalassaemia screening programme in Malaysia.
  4. Salwati Shuib, Sharifah Noor Akmal, Zarina Abdul Latif, Nor Zarina Zainal Abidin, Zubaidah Zakaria
    Medicine & Health, 2006;1(1):45-52.
    MyJurnal
    In this report we demonstrate the role of fluorescence in situ hybridisation (FISH) and conventional cytogenetic methods in clinically and cytogenetically confirmed cases of microdeletion syndromes. A total of nine cases were referred to the Cytopathology and Cytogenetic Unit, Hospital Universiti Kebangsaan Malaysia (HUKM) from 2002 to 2004. They include three Prader-Willi syndrome, three DiGeorge syndrome, one Williams syndrome, one Miller-Dieker syndrome and one Kallmann syndrome. Blood samples from the patients were cultured and harvested following standard procedures. Twenty metaphases were analysed for each of the cases. FISH analysis was carried out for all the cases using commercial probes (Vysis, USA): SNRPN and D15S10 for Prader-Willi syndrome, LIS1 for Miller Dieker syndrome, ELN for Williams syndrome, KAL for Kallmann syndrome, TUPLE 1 and D22S75 for DiGeorge syndrome. Conventional cytogenetic analysis revealed normal karyotypes in all but one case with structural abnormality involving chromosomes 9 and 22. FISH analysis showed microdeletions in all of the nine cases studied. This study has accomplished two important findings ie. while the FISH method is mandatory in ruling out microdeletion syndromes, conventional cytogenetics acts as a screening tool in revealing other chromosomal abnormalities that may be involved with the disease.
  5. Lina Izzati, A., Haszianaliza, H., Zar, C.T.
    Medicine & Health, 2018;13(1):49-70.
    MyJurnal
    Malaysia is blessed to have several natural products. Since past decades, studies have been carried out to discover the highly effective anti-atherosclerotic supplements. The phytoestrogens and soy proteins have gained much attention, over the years. Several literature highlighted the therapeutic effect of alternative medicines on atherosclerosis. However, the overall anti-atherosclerotic mechanism of isoflavones regardless of menopause or postmenopausal state was not discussed, to date. This review aimed to summarize the molecular mechanism of different types of alternative medicines for the treatment of atherosclerosis in general population. The herbs commonly used in the treatment of atherosclerosis are garlic (Allium sativum), pomegranate (Punica granatum), ginger (Zingiber officinale), cinnamon (Cinnamomum spp.) and green tea (Camellia sinenses). Nevertheless, the palm oil, coconut oil, olive oil and omega-3 also plays significant role in attenuating the risk of atherosclerosis by decreasing the LDL level, increasing the endothelial nitric oxide synthase (eNOS), scavenging the free radicals and also decreasing the inflammatory process. Daidzein improves atherosclerotic changes by activating the NF-ĸB pathway and regulating the expression of inflammatory cytokines. Genistein reduces the monocyte-endothelial cell and adhesion molecules secretion via cAMP/PKA pathway, it decreases the inflammatory response via initiation of nuclear factor erythroid 2-related factor (Nrf2)/ heme oxygenase-1 (HO-1) pathway. Conclusively, we recognized that alternative medicines demonstrate remarkable therapeutic efficacy for the treatment of atherosclerosis in patients of all ages.
  6. Kumeshini, S., Nurshazana, A., Faizah, O., Kamsiah, J., Srijit, D., Zar Chi, T.
    Medicine & Health, 2016;11(2):171-180.
    MyJurnal
    Menopause, a cessation of menstrual bleeding, is directly related to serious
    illness confined to cardiovascular disease (CVD) such as hypertension and stroke. The oestrogen withdrawal and consumption of high cholesterol diet has
    a detrimental effect on cardiovascular function and metabolism in menopausal
    women. The present study was aimed to develop the hypertensive rat model in
    the ovariectomised Sprague Dawley rats fed with 2% cholesterol diet following a
    short term period. Eighteen Sprague-Dawley rats were randomly divided into three
    groups; Sham control (sham), ovariectomized control fed with normal diet (Ovx
    +N), ovariectomized rats fed with 2% cholesterol diet (Ovx + Cho) for four weeks.
    At the end of the study, the food intake and body weight were measured. The
    systolic, diastolic and mean blood pressure was determined. OVX+Cho showed
    significant increase in physiological changes such as body weight and food intake
    compared to Ovx+N group. The significant increase in systolic, diastolic and mean
    blood pressure was observed in Ovx+Cho group. Based on the above findings,
    it is believed that feeding cholesterol diet for four weeks (short term) results in
    significant increase in the blood pressure, food intake and body weight in the
    ovariectomised rats.
  7. Putri Shuhaili, S., Haszianaliza, H., Muhammad Fairuz, A., Zar Chi, T.
    Medicine & Health, 2016;11(2):105-116.
    MyJurnal
    In recent years, there is a growing enthusiasm in honey which has potential health benefits, disease prevention and serves as substitution of modern medicine. Honey is a natural sweet product which is produced by honey bees from floral nectar and has been traditionally used to treat several diseases such as cardiovascular disease, diabetes mellitus, cancer and Alzheimer’s disease since ancient times. Gelam honey (GH) is one of the Malaysian wild monofloral honey which is widely used as traditional medicine by the local population. GH is enriched with several therapeutic properties namely, antioxidative, antiinflammatory, anticancer and accelerated wound healing activities. However, there is lack of literature focusing on the overall effects of GH and its medicinal value. This review paper summarises the phytochemistry characteristics of GH along with its therapeutic values which lead to a broad spectrum idea on several diseases. A concise clarification on the therapeutic properties of GH is expected to provide new knowledge to the scientific society in the era of modern globalization.
  8. Asma, A.A.A., Rohaya, M.A.W., Juhaida, S., Badiah, B., Zaleha, S.
    Medicine & Health, 2020;15(2):96-107.
    MyJurnal
    Rawatan ortodontik boleh menjejaskan keseimbangan mikrobiota oral yang memainkan peranan utama dalam etiologi penyakit periodontium. Kajian klinikal prospektif ini bertujuan untuk menilai kesihatan periodontal dan profil mikrobiologi pesakit periodontal yang sihat (Kumpulan 1) dan yang telah stabil (Kumpulan 2) selama tiga bulan pertama semasa rawatan ortodontik. Aplian ortodontik atas dan bawah tetap dipasang. Kesihatan periodontium dinilai menggunakan skor plak (PS), pendarahan pada probing (BOP) dan kedalaman poket (PD). 29 tapak telah diambil untuk persampelan plak subgingival. Sampel plak diinokulasikan pada agar Trypticase Soya Darah (TSBA) dan agar Trypticase Soya Bacitracin Vancomycin (TSBV) untuk penilaian aerob, anaerob, bakteria berpigmen hitam (BPH) dan Aggregatibacter actinomycetemcomitans. Semua ukuran diambil sebelum pendakap gigi dipasang (T0), 1 minggu (T1), 1 bulan (T2) dan 3 bulan selepas dipasang pendakap gigi (T3). Secara umumnya, kesihatan periodontium dalam kedua-dua kumpulan hampir sama. Selepas 1 minggu, bilangan aerob adalah lebih tinggi dalam Kumpulan 1 (88%) manakala anaerob adalah lebih tinggi dalam Kumpulan 2 (45%). A. actinomycetemcomitans lebih tinggi dalam Kumpulan 1 pada T0 dan T1 tetapi jauh lebih tinggi dalam Kumpulan 2 di T3. BPH adalah minimal pada setiap masa dengan tiada perbezaan signifikan. Oleh itu, semasa 3 bulan pertama rawatan ortodontik dijalankan, terdapat perubahan ketara dalam bilangan aerob-anaerob pada kedua-dua pesakit periodontal yang sihat dan stabil. Bakteria patogenik akan meningkat semasa rawatan awal ortodontik.

  9. Mohd Shahrol, A.W., Mohd Hasni, J., Zaleha, M.I.
    Medicine & Health, 2020;15(2):187-214.
    MyJurnal
    Pengetahuan mengenai pendedahan plumbum (Pb) di kalangan kanak-kanak masih kurang baik di kalangan ibu bapa di Malaysia. Tujuan utama kajian ini adalah untuk menterjemahkan versi Bahasa Inggeris Chicago Lead Knowledge Test (CLKT) yang asal ke dalam Bahasa Melayu (bahasa tempatan) dan disesuaikan dengan latar belakang budaya negara ini. Langkah-langkah terjemahan adalah sistematik dan berdasarkan ‘International Society for Pharmacoeconomics and Outcome Research’ (ISPOR) iaitu persediaan, terjemahan ke depan, perangkuman, terjemahan mundur, penilaian terjemahan mundur, penyelarasan, pembahasan kognitif, penilaian pembahasan kognitif dan semakan akhir, pembacaan pruf, dan laporan akhir. Jumlah item adalah kekal 24, di mana satu item dihapus, satu item disemak semula, dan satu item ditambah ke dalam borang soalan kaji selidik. Keempat-empat tema iaitu pengetahuan umum, pendedahan, pencegahan dan pemakanan dalam soal selidik ini dikekalkan. Skala respon, skema jawapan, dan sistem pemarkahan adalah tetap sama. Sebilangan besar item mempunyai terjemahan langsung ke bahasa Melayu kecuali item 5, 7, 12, 20, dan 22. Purata (+SD) dan julat interkuartil (IQR) untuk skor keseluruhan pengetahuan adalah 9.50 + 2.45 markah dan 5 markah masing-masing. Skor terendah adalah 4 markah dan skor tertinggi ialah 13 markah. Ini merupakan satu-satunya kajian yang menggunakan pendekatan sistematik dan standard dalam terjemahan dan penyesuaian budaya di Malaysia. Terjemahan CLKT dan penyesuaian budaya di Malaysia akan menyumbang kepada penyelidikan semasa yang lain terutamanya mengenai pendedahan Pb di kalangan kanak-kanak.

  10. Khairani, O., Norazua, R., Zaiton, A.
    Medicine & Health, 2007;2(1):80-85.
    MyJurnal
    Smoking in adolescence is a great health concern as it is related to many chronic diseases and mortality in later life. It is also associated with other high-risk behaviours among adolescents. The objective of this study was to determine the prevalence and reasons for smoking among upper secondary schoolboys. This was a cross-sectional study conducted among Form 4 and Form 5 students. Three schools in the District of Hulu Langat were identified and students from these schools were selected randomly. The students were given a self-administered questionnaire. A total of 343 students were included in the study. The prevalence of smoking among the schoolboys was 37%. Although the majority of them initiated smoking between 13-15 years old (67%), 21% of them began smoking below 12 years of age (primary school). There was a significant association between adolescent smoking and smoking among family members (p<0.05). The common reasons reported for
    initiating smoking were curiosity (69%) and peer pressure (51%). However, the common reasons for continuing smoking were stress (70%) and addiction (49%). Schoolwork was reported as the most important factor which contributed to their stress. Smoking prevention programmes should begin early in primary schools. Adolescents should be educated on effective coping strategies in managing stress and learning to be assertive.
    Keywords: smoking, adolescents, secondary school, reasons.
  11. Adeeb, N., Nur-Azurah, A.G., Ong, F.B., Seri, S.S., Shamsuddin, K., Noor-Aini, M.Y., et al.
    Medicine & Health, 2008;3(1):59-68.
    MyJurnal
    Cancers of the breast and cervix made up 30.4% and 12% of all cancer cases in Malaysia. Thus screening for reproductive organ cancers as women approached menopause becomes exceedingly important. The study reports the baseline assessment tests of 495 disease free urban Malaysian women aged 45 years and above who volunteered in a healthy lifestyle intervention study. The sample comprised of 58.0% premenopaused and 42.0% postmenopaused women with an average age of 51.27±5.35 years old. Over two thirds were Chinese followed by Malays and Indians. Overall, abnormal Pap smears were seen in 7.6% comprising of 1.3% cervical intraepithelial neoplasia (CIN), 6.1% human papilloma virus (HPV) infection and 0.2% atypical squmous cells of undetermined significances (ASCUS). Yeast and other infections were found in 6.9% and 1.9% respectively. Comparatively, postmenopausal women had a 2.8 fold higher cancerous changes whereas premenopausal women had a higher infection rate, 11.8% vs. 4.7% respectively (p=0.024) with comparable HPV infection rates in both. This study found 1.3% had breast cancer (BC) with 3.6% requiring a biopsy while 3.4% needed regular follow up. Postmenopaused women had more abnormal mammograms (p
  12. Ng CWK, Hanizasurana H, Nor Azita AT, Nor Fariza N, Zabri K
    Medicine & Health, 2016;11(2):313-318.
    MyJurnal
    Cytomegalovirus (CMV) retinitis occurs predominantly in Human Immunodeficiency Virus (HIV) -infected patients. It was also reported in HIV-seronegative patients with systemic autoimmune disorder requiring systemic immunosuppression, organ or bone marrow transplantation, haematological or breast malignancy receiving chemotherapy, ocular diseases following intraocular or periocular corticosteroid injection, diabetes mellitus and Good syndrome. However, CMV retinitis in patients with concurrent dermatomyositis and malignancy has not been previously reported. It has not been reported in cancer other than haematological or breast malignancy, or in cancer patient prior to chemotherapy. We report a case of 40-year-old HIV-seronegative woman with underlying dermatomyositis and lung malignancy who developed right CMV retinitis which relapsed after recommencement of immunosuppressant. Both episodes of CMV retinitis were successfully treated after taken her immunocompromised state into consideration.
  13. Kuharajan R., Mohd Yazid, B., Ohnmar, H., Yuliawiratman, B.S.
    Medicine & Health, 2019;14(1):172-182.
    MyJurnal
    Charcot arthropathy is a devastating degenerative condition which affects one or more joints that is marked by joint instability, hypermobility resulted from peripheral nerve damage. This was a retrospective study performed on patients who were diagnosed with Charcot arthropathy and those underwent surgery for hindfoot arthrodesis at Universiti Kebangsaan Malaysia Medical Centre from January 2011 until June 2016. The objective of the study was to review the feasibility of an algorithm in managing Charcot arthropathy and to assess functional, clinical and radiographic outcomes of Charcot arthropathy joints treated with hindfoot arthrodesis, at least 6 months follow-up using standardized and internationally accepted scoring systems, which were the American Orthopaedic Foot and Ankle Surgery (AOFAS) score and SF36. A total of 16 patients were included in this study of which 4 (25%) were males and 12 (75%) were females. The mean age was 58.1 (20-71) years. There was equal distribution for side of fusion. We achieved union in 13 out of our 16 (81%) cases followed up. Out of the 3 patients with non-union, 2 were attributed to deep infections. The AOFAS scores for hindfoot and midfoot showed significant improvement post operatively with p-value
  14. Siti, N. I., Kavireshna, K., Zafirah, M. R., Foo, Y. I. W., Rabiatul Adawiyah, R., Salina, H., et al.
    Medicine & Health, 2017;12(2):259-274.
    MyJurnal
    Model tiga dimensi (3D) menyerupai ciri-ciri persekitaran tisu asli, justeru
    morfologi dan isyarat-isyarat sel daripada kultur 3D selalunya lebih menyerupai
    fisiologi asal berbanding sel kultur dua dimensi (2D). Diketahui juga, rembesan
    sel mempunyai kesan parakrin kepada pertumbuhan sel-sel lain. Dalam kajian
    ini, pengkulturan fibroblast hidung menggunakan system kultur sel 3D telah
    dioptimumkan dan kesan bahan rembesan (BR) daripada kultur 3D terhadap kadar
    pertumbuhan dan perlindungan sel telah dikaji. Fibroblas hidung dipencilkan
    daripada turbinate hidung manusia. Mikrosfera yang sesuai telah dipilih melalui
    pengkulturan fibroblast pemindahan ke-3 pada pelbagai jenis mikro sferapolisterin
    PolyGEM™. Kemudian, sel-sel telah dikulturkan pada mikrosfera yang terpilih
    menggunakan system kultur 3D dan media terkondisi (MT) telah dikumpulkan.
    Media terkondisi tiga dimensi (MT3D) telah ditambah kepada fibroblast untuk
    mengkaji kadar perlekatan sel, kadar proliferasi, dan perlindungan sel terhadap
    kesitotoksikan Centella asiatica. Asai protein asid bicinchonic dijalankan untuk
    mengetahui kuantiti protein di dalam BR. Elektroforesis gel poliakrilamida-Sodium
    Dodesil Sulfat (SDS-PAGE) telah dilakukan untuk memperoleh profil awal protein
    dan membandingkan profil MT3D dengan protein media terkondisi dua dimensi
    (MT2D). Kajian ini menunujukkan MT3D tidak menggalakkan perlekatan dan
    proliferasi sel secara signifikan. BR didapati memberikan perlindungan sel yang
    signifikan pada fibroblast hidung terhadap kesitotoksikan Centella asiatica. MT3D
    mempunyai kepekatan protein yang lebih tinggi berbanding MT2D. SDS-PAGE
    menunjukkan MT3D mempunyai 3 jalur ekslusif manakala MT2D mempunyai 4 jalur eksklusif. Kajian masa depan harus dijalankan keatas penggunaan BR
    fibroblast hidung untuk perlindungan sel terhadap agen-agen yang memudaratkan
    di alam sekitar dan produk herba yang sitotoksik.
  15. Nur Ain, J., Ainaa Diyana, S., Nur Hazirah, R., Nur Fathiah, A.S., Fairus, A., Teoh, S.l., et al.
    Medicine & Health, 2018;13(1):165-174.
    MyJurnal
    Piper betle (PB) leaves have been traditionally used in many Asian countries for the healing of wounds and other ailments. The aim of this study was to examine the wound healing effect of PB on skin injury-inflicted Sprague-Dawley rats. Eighteen male Sprague-Dawley rats were inflicted with wounds and then divided into three groups consisting of a control (normal wound healing without dressing), saline (wound healing aided by gauze soaked in saline) and PB (gauze impregnated with PB paste) dressings. Wounds were created on the back of rats with 6-mm sterilized punch biopsy needle. Wounds were examined on days 3, 5, 7, 9, 11 and 13 for dryness, exudation, and scar formation. Rats were sacrificed on day 14 and the granulation tissue formed on the wound was then excised for histological examination using Hematoxylin and Eosin staining. The formation of granulation tissue in rats treated with PB showed higher progress to wound closure with shorter days (on day 3) compared to the control and saline group (on day 5). PB dressing minimized the formation of scar tissue significantly (p
  16. Saba, Z.H., Suzana, M., Yasmin Anum, M.Y.
    Medicine & Health, 2013;8(1):3-18.
    MyJurnal
    Madu adalah bahan semulajadi yang dihasilkan oleh lebah madu, Apis mellifera, berpunca daripada madu yang diambil dari bunga yang berkembang atau cecair dari pokok dan tumbuhan yang dikenali sebagai madu nektar dan madu serangga masing-masing. Ia adalah larutan tepu gula, yang kaya dengan protein, mineral, vitamin, asid organik dan polifenol. Madu mempunyai pelbagai khasiat, sifat penyembuhan dan profilaktik disebabkan oleh komponen-komponen yang terkandung di dalamnya. Madu mempunyai beberapa khasiat kesihatan seperti penyembuhan luka, antimikrob, antioksidan dan potensi anti-radang. Ulasan ini adalah berkaitan komposisi nutrien, antioksidan dan kesan terapeutik madu dengan penekanan kepada madu di Malaysia.
  17. Arshad, N.A., Nellysha Namela, M.A.K., Teoh, S.L., Yahaya, M.F.
    Medicine & Health, 2019;14(2):30-40.
    MyJurnal
    Brain health deterioration is a worldwide health epidemic. Alzheimer’s disease and other dementias are among the most expensive diseases to treat, with direct care expenses exceeding those for cancer and heart disease. The consumption of overly processed food products over the time has contributed to the increasing number of health deterioration at an earlier age. Honey has been known worldwide through history to be one of the purest, and popular natural product to be consumed as a health booster notably due to its antioxidant and phenolic contents. In the recent decade, many studies were perfomed to understand the biological process of cellular insult to the brain and ways to halt or slow down the process using natural products. Honey was shown in some of these studies as having the potential that may reduce or slow down these neural degenerations. Based on recent studies conducted on the use of honey and its effects on the brain physiologically, and anatomically, the results showed that honey holds potential in halting or even treating brain health deteriorations.
  18. Puwaneswarry M., Nathratul Ayeshah, Z., Gaaitheri K., Lim, K.Q., Tang, S.L., Ng, C.G., et al.
    Medicine & Health, 2020;15(2):262-275.
    MyJurnal
    Dalam situasi wabak COVID-19 ketika ini, maklumat yang tidak disahkan telah membanjiri platform media sosial, lalu meningkatkan tahap kegelisahan dan gejala kemurungan di kalangan orang ramai. Penyelidikan ini bertujuan untuk menilai hubungan antara pengetahuan, sikap, dan amalan (KAP) yang berkaitan dengan COVID-19 dan kesan psikologinya, seperti kegelisahan dan gejala kemurungan pada rakyat Malaysia. Tinjauan dalam talian telah dilaksanakan dari 8-22 April 2020, yang melibatkan 227 responden. Set soal selidik tersebut merangkumi ciri-ciri sosiodemografi, soal selidik KAP - 11 soalan mengenai pengetahuan (K1-K11), 3 soalan mengenai sikap (A1-A3), 4 soalan mengenai amalan (P1-P4), bersama dengan Soal Selidik ‘Patient Health Questionnaire (PHQ-9)’ dan ‘Generalized Anxiety Disorder (GAD-7) Scale’. Untuk mentafsir hasil penyelidikan, analisis deskriptif, ujian chi-square dan analisis teori respons barang telah dilaksanakan. Peratusan responden yang menjawab soalan penyataan benar dari komponen Pengetahuan (K1-K3) dengan betul adalah hampir 100%. Walau bagaimanapun, item yang menguji maklumat palsu COVID-19 (K4-K11) berkisar dari 38.8% hingga 95.2%. Satu-satunya item dari komponen Sikap yang kurang dari 95% dijawab dengan betul adalah A1 (78.9%), mengenai penggunaan topeng muka. Dari komponen Latihan, lebih daripada 80% responden mengenal pasti bahawa mereka menggunakan media sosial untuk mendapatkan kemas kini mengenai COVID-19 sepanjang masa. Ini merupakan sesuatu yang membimbangkan kerana kebanyakan maklumat yang beredar di media sosial belum disahkan oleh pihak berwajib, yang mungkin menyebabkan, dan terus-menerus memperkuat maklumat palsu yang menimbulkan kegelisahan. Kajian ini menunjukkan bahawa skor KAP keseluruhan yang lebih rendah, terutamanya untuk item komponen Pengetahuan, berkaitan langsung dengan tahap kegelisahan dan kemurungan yang lebih tinggi.
  19. Yasmin Anum, M.Y., Shahriza, Z.A., Looi, ML, Shafina Hanim, M.H., Harlianshah, H., Noor Aini, A.H., et al.
    Medicine & Health, 2008;3(2):263-274.
    MyJurnal
    Ginger extract has been reported previously by our group to exhibit anticancer and an-tioxidant effects by reducing tumour burden and lipid peroxidation respectively in he-patocarcinogenesis induced rats. The current study examined the expression of pro-apoptotic protein caspase-8 and anti-apoptotic protein Bcl-2 in hepatocarcinogenesis treated rats. Thirty normal male Wistar rats were divided into 5 groups based on the diet given: i) control (normal rat chow), ii) olive oil, iii) ginger extract (100mg/kg body weight), iv) choline deficient diet + ethionine, CDE (to induce liver cancer) and v) CDE+ ginger extract. Rats were killed at week 8, and liver tissues were excised for immuno-histochemical study to identify pro-apoptotic and anti-apoptotic proteins, caspase-8 and Bcl-2. The observation on H&E staining confirmed the CDE diet induced liver can-cer as indicated by the presence of numerous oval cells. Identification of Bcl-2 expres-sion showed that 91.6% (11/12) of the samples from the CDE group revealed positive staining while treatment with ginger extract however inhibited the expression with only 8.4% (1/12) samples showing positive staining for Bcl-2. As for caspase-8 protein, 41.7% (5/12) of the samples from CDE group showed positive staining, which in-creased to 100% (12/12) with ginger extract treatment. Our findings suggest that gin-ger extract has an anticancer effect by inducing apoptosis in liver cancer cells via up-regulation of the expression of pro-apoptotic protein, caspase-8 and down-regulation of the expression of anti-apoptotic protein Bcl-2.
  20. Farahani, A.S.R., Zakiah, J., Abdul Rahman, M., Karsani, S.A., Wan, Ngah Wz
    Medicine & Health, 2008;3(2):256-262.
    MyJurnal
    Gamma-tocotrienol (GTT) has been shown to exhibit significant antitumor activity in a variety of tumor cells. Previous findings have demonstrated that GTT had antiprolifera-tive effects on a liver cancer cell line (HepG2) with an IC50  value of 170μM. In this study, two dimensional gel electrophoresis (2DE) was used to determine changes in protein expression in HepG2 cell line following treatment with GTT. The ultimate aim is to identify the possible molecular mechanisms involved in GTT antitumor activity. This study is focused on obtaining a 2DE protein profile for HepG2 cell line with and without
    GTT treatment. In the preliminary analysis  of the resulting 2DE profiles, 18 protein spots were found to be differentially expressed in cells treated with GTT. This observa-tion is confirmed by extending the analysis  to a larger sample size. By studying the effects of GTT treatment on differential protein expression in HepG2 cells the underly-ing mechanisms involved in the antitumor activity of GTT may be elucidated.
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