Displaying publications 1 - 20 of 106 in total

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  1. Abubakar M. Umar, Tham, Lik Gin, Natarajan Perumal, Nur Adeela Yasid, Hassan Mohd Daud, Mohd Yunus Shukor
    MyJurnal
    Acetylcholinesterase (AChE) is usually used as an inhibitive assay for insecticides. A lesserknown
    property of AChE is its inhibition by heavy metals. In this work, we evaluate an AChE
    from brains of Clarias batrachus (catfish) exposed to wastes from aquaculture industry as an
    inhibitive assay for heavy metals. We discovered that the AChE was inhibited completely by
    Hg2+, Ag2+, Pb2+, Cu2+, Cd2+, Cr6+ and Zn2+ during initial screening. When tested at various
    concentrations, the heavy metals exhibited exponential decay type inhibition curves. The
    calculated IC50 (mg/L) for the heavy metals Ag2+, Cu2+, Hg2+, Cr6+ and Cd2+ were 0.088, 0.078,
    0.071, 0.87 and 0.913, respectively. The IC50 for these heavy metals are comparable, and some
    are lower than the IC50 values from the cholinesterases from previously studied fish. The assay
    can be carried out in less than 30 minutes at ambient temperature.
    Matched MeSH terms: Catfishes
  2. Adebiyi FA, Siraj SS, Harmin SA, Christianus A
    Fish Physiol Biochem, 2013 Jun;39(3):547-57.
    PMID: 23010937 DOI: 10.1007/s10695-012-9718-x
    Plasma sex steroid hormonal profile and gonad histology were correlated to study the annual reproductive cycle of Hemibagrus nemurus. Hormones were measured by Enzyme Linked Immunosorbent Assay. Gonad tissues were observed by using light microscopy. The highest testosterone (T) value for male was observed in November and that of female was in October. 11-ketotestosterone (11-KT) and 17β-estradiol (E2) levels were highest in June and November, respectively. Hormonal profiles of T, 11-KT and E2 showed several peaks which indicated a non-seasonal pattern. There were significant differences (p 
    Matched MeSH terms: Catfishes/anatomy & histology; Catfishes/blood*
  3. Adebiyi FA, Siraj SS, Harmin SA, Christianus A
    J Exp Zool A Ecol Genet Physiol, 2011 Nov 1;315(9):536-43.
    PMID: 21898849 DOI: 10.1002/jez.702
    Hemibagrus nemurus is a riverine catfish with high economic and nutritive values. Investigations on ovarian development of this fish were carried out to determine the mode of ovarian development and describe the oocyte developmental stages. Histological studies were done on ovaries using light microscopy and scanning electron microscopy. Fish were sampled monthly for a period of six months (August 2009 to January 2010). The mean oocyte diameter (OD) ranged from 871 ± 161.41 µm to 1,167 ± 26.77 µm and the highest OD was in November. Oocyte size-frequency distribution showed a polymodal distribution. The mean gonadosomatic index (GSI) ranged from 1.14 ± 0.87% to 7.06 ± 1.40% and highest GSI was in November. The ovaries exhibited three phases of oocyte growth, which were primary growth, secondary growth and maturation phases. Based on histological criteria, the oocyte developmental stages were divided into seven stages as chromatin nucleolar, early perinucleolar, late perinucleolar, cortical alveolar, vitellogenesis, mature oocyte and germinal vesicle migration stages. All the seven stages of oocyte development were observed in the ovaries. Oogonia were always present throughout the developmental stages. The ovaries had more than two stages of oocyte development. This is the first report on the mode of ovarian development of H. nemurus. These findings indicated that H. nemurus has asynchronous mode of ovarian development and is capable of spawning several times in a year under favourable conditions.
    Matched MeSH terms: Catfishes/anatomy & histology; Catfishes/growth & development*
  4. Ahmad R, Zaini IZ, Mohamed NA, Roslanuddin MS
    PMID: 24050106
    Peripheral vascular injuries result from penetrating or blunt trauma to the extremities. We describe here an unusual cause of a radial artery injury by the spine from the pectoral fin of Pangasius sutchi in a 51 year-old man and we review the literature.
    Matched MeSH terms: Catfishes*
  5. Alam L, Mohamed CA
    Environ Health, 2011 May 20;10:43.
    PMID: 21595985 DOI: 10.1186/1476-069X-10-43
    BACKGROUND: Po²¹⁰ can be accumulated in various environmental materials, including marine organisms, and contributes to the dose of natural radiation in seafood. The concentration of this radionuclide in the marine environment can be influenced by the operation of a coal burning power plant but existing studies regarding this issue are not well documented. Therefore, the aim of this study was to estimate the Po²¹⁰ concentration level in marine organisms from the coastal area of Kapar, Malaysia which is very near to a coal burning power plant station and to assess its impact on seafood consumers.

    METHODS: Concentration of Po²¹⁰ was determined in the edible muscle of seafood and water from the coastal area of Kapar, Malaysia using radiochemical separation and the Alpha Spectrometry technique.

    RESULTS: The activities of Po²¹⁰ in the dissolved phase of water samples ranged between 0.51 ± 0.21 and 0.71 ± 0.24 mBql⁻¹ whereas the particulate phase registered a range of 50.34 ± 11.40 to 72.07 ± 21.20 Bqkg⁻¹. The ranges of Po²¹⁰ activities in the organism samples were 4.4 ± 0.12 to 6.4 ± 0.95 Bqkg⁻¹ dry wt in fish (Arius maculatus), 45.7 ± 0.86 to 54.4 ± 1.58 Bqkg⁻¹ dry wt in shrimp (Penaeus merguiensis) and 104.3 ± 3.44 to 293.8 ± 10.04 Bqkg⁻¹ dry wt in cockle (Anadara granosa). The variation of Po²¹⁰ in organisms is dependent on the mode of their life style, ambient water concentration and seasonal changes. The concentration factors calculated for fish and molluscs were higher than the recommended values by the IAEA. An assessment of daily intake and received dose due to the consumption of seafood was also carried out and found to be 2083.85 mBqday⁻¹person⁻¹ and 249.30 μSvyr⁻¹ respectively. These values are comparatively higher than reported values in other countries. Moreover, the transformation of Po²¹⁰ in the human body was calculated and revealed that a considerable amount of Po²¹⁰ can be absorbed in the internal organs. The calculated values of life time mortality and morbidity cancer risks were 24.8 × 10⁻⁴ and 34 × 10⁻⁴ respectively which also exceeded the recommended limits set by the ICRP.

    CONCLUSIONS: The findings of this present study can be used to evaluate the safety dose uptake level of seafood as well as to monitor environmental health. However, as the calculated dose and cancer risks were found to cross the limit of safety, finding a realistic way to moderate the risk is imperative.

    Matched MeSH terms: Catfishes/metabolism
  6. Alias MIM, Hambali K, Amir A, Fauzi N, Hassin H, Yin SA
    Trop Life Sci Res, 2019 Jan;30(1):161-167.
    PMID: 30847039 MyJurnal DOI: 10.21315/tlsr2019.30.1.10
    A survey was conducted to determine the diversity of fish in Pergau Lake, Jeli, Kelantan, Malaysia from 2 September to 18 October of 2016. Fish samples were collected at seven random sampling stations around the lake by using setting trap method. The collected samples were preserved in 10% formalin solution and delivered to the laboratory for further identification process. Fourteen species, namely as Hemibagrus nemurus, Hampala macrolepidota, Clarias batrachus, Channa striata, Cyprinus carpio, Poropuntius smedleyi, Pangasius sp., Oreochromis niloticus, Oreochromis mossambicus, Leptobarbus hoevenii, Neolissochilus hexagonolepis, Tor tambroides, Osteochilus hasselti and Neolissochilus soroides, comprising of six families were collected during this study. From this study, family of Cyprinidae was the dominant species in Pergau Lake with exactly 50% of catch percentage, followed by family of Bagridae (29%), Cichlidae (10%), Clariidae (5%), Channidae (3%) and Pangasiidae (3%). This study shows that there is still a diversity of fish species in Pergau Lake, showing that the water and its environmental condition is very good.
    Matched MeSH terms: Catfishes
  7. Alih RA, Solomon SG, Olufeagba SO, Cheikyula JO, Abol-Munafi AB, Okomoda VT
    Zygote, 2022 Feb;30(1):125-131.
    PMID: 34176523 DOI: 10.1017/S0967199421000411
    The study sought to investigate the chronology of events and timing of embryogenesis, as well as breeding performances of three strains of Heterobranchus longifilis from Nigeria. Fish samples were collected from Benue River in Makurdi, Niger River in Onitsha, and Rima River in Sokoto for this study. Induced spawning of the strains was carried out so that egg development could be tracked from fertilization to hatching using a simple microscope. The microphotographs obtained showed that the embryogenesis of the strains followed a similar pattern to those of other members of the family Clariidae, however with changes occurring in the specific timing of the sequences of events (i.e. interstrain and interspecies differences). When the different strains were compared, the study noted similarities (P > 0.05) in the overall breeding performance (except for fertilization rate), survival at different stages of development, timing of embryogenesis, and larvae characteristics. The outcomes of this study, therefore, provide baseline information on what genetic improvement of the species through strain crossing can be attempted in future studies.
    Matched MeSH terms: Catfishes*
  8. Amin OM, Chaudhary A, Heckmann RA, Ha NV, Singh HS
    Acta Parasitol, 2019 Dec;64(4):779-796.
    PMID: 31332657 DOI: 10.2478/s11686-019-00102-3
    BACKGROUND: Most (82%) of the 46 recognized species of Acanthogyrus (Acanthosentis) Verma and Datta, 1929 are known from Asian freshwater fishes. Only three species of Acanthosentis are known from marine or brackish water fishes from India and Pakistan. We have discovered another marine species of Acanthosentis in the Pacific Ocean, off Vietnam.

    PURPOSE: The purpose is to describe the new species morphologically and molecularly and provide new information of its evolutionally relationships with other species of the subgenus.

    METHODS: Standard methods of collection and examination of marine hosts, processing and illustrating of specimens, and taxonomic identification of parasites using the extensive collection of the lead author were used. Specimens were further studied using energy-dispersive X-ray analysis and ion sectioning of hooks, SEM analysis, and molecular sequencing. Type specimens were deposited at the Harold W. Manter Lab. collection, Lincoln, Nebraska.

    RESULTS: Acanthogyrus (Acanthosentis) fusiformis n. sp. is described from the catfish, Arius sp. (Ariidae: Siluriformes) off the Pacific Coast of Vietnam at Bac Lieu in the Gulf of Thailand. The three other marine Indian species include A. (A.) arii Bilqees, 1971 which is also described from a similar catfish, Arius serratus Day off the Karachi coast in the Arabian Sea, Indian Ocean. Our new species from Vietnam is distinguished from the other 46 species by a combination of characters including a small fusiform trunk, complete circles of small hollow spines covering the entire trunk, prominent double apical organs often extending posteriorly past posterior hooks, middle and posterior hooks of equal size slightly smaller than anterior hooks, large neck continuous with the outline of the proboscis without distinct separation, big drop-shaped cephalic ganglion, extension of the proboscis receptacle anteriorly past the base of the proboscis up to the insertion point of the posterior hooks, presence of two para-receptacle structures (PRSs), free unattached thick lemnisci, short female reproductive system with filamentous attachment of the distal end of the uterine bell to the ventral body wall, and small narrowly ellipsoid eggs with thickened polar ends. Partial sequences of the 18S and internal transcribed spacers (ITS1-5.8S-ITS2) of ribosomal RNA were generated and used for phylogenetic analyses to confirm the taxonomic identity of Acanthogyrus (Acanthosentis) fusiformis n. sp.

    CONCLUSIONS: We describe unique morphological features of A. fusiformis never before known in the subgenus Acanthosentis. The uniqueness of A. fusiformis is further demonstrated by its EDXA fingerprint characterized by high levels of calcium and phosphorous in hooks. The zoogeography of species of Acanthosentis is elucidated in the Indian subcontinent, the Caribbean, China, and Africa. Molecular data have been available only in few species of Acanthogyrus (Acanthosentis) to date on GenBank database. For 18S, only two sequences from unknown Acanthosentis sp. from India are available, while for the ITS1-5.8S-ITS2 region, only sequences of A. cheni from China and of two unidentified species from Malaysia are available. Additional studies of species of Acanthosentis based on morphological and molecular genetic data will be needed to reconstruct the evolutionary history and phylogenetic affinities of this group of acanthocephalans.

    Matched MeSH terms: Catfishes/parasitology*
  9. Amiza, M.A, Kang, W.C.
    MyJurnal
    The effect of the addition of different concentrations of chitosan (0–2.0% w/w) on the gelling properties of surimi gels made from African catfish (Clarias gariepinus) was tested. Lipid oxidation, total volatile basic nitrogen (TVB-N), and aerobic plate count (APC) changes during 20 days of storage at 4oC also were evaluated. Surimi gels with 1.5% (w/w) chitosan added exhibited the highest improvement in gel strength (58.92%), whiteness (13.18%), and water holding capacity (36.8%). Incorporation of 2.0% (w/w) chitosan in gels resulted in the lowest TVB-N value (36.63 mg N/100 g) at the end of the 20 days storage period. Both the peroxide values and the 2-thiobarbituric acid values increased more slowly in the chitosan-treated gels than in the control gel during the storage period. Chitosan at concentrations of 1.75% and 2.0% (w/w) conferred the best antioxidant effect on catfish surimi gels and resulted in a significant reduction in APC. Based on the microbiological acceptability limit (106 cfu/g), the shelf life
    of surimi gels with 1.75% and 2.0% (w/w) chitosan was extended to 12 days in refrigerated storage at 4oC, whereas the other samples lasted only 8 days. Hence, the addition of 1.5–2.0% (w/w) chitosan is a promising approach for the preparation of catfish surimi gels, as it improves texture, prevents lipid oxidation, and inhibits microbial growth.
    Matched MeSH terms: Catfishes
  10. Amiza, M.A., Nurul Ashikin, S., Faazaz, A.L.
    MyJurnal
    This study aims to determine the combined effects of hydrolysis time, temperature, pH and ratio of enzyme to substrate on the degree of hydrolysis (DH) of silver catfish frame using Response Surface Methodology. The proximate compositions of silver catfish frame and silver catfish hydrolysate powder were determined as well. The effects of independent factors were described using a three-level factors Face Centered Central Composite design. The suggested hydrolysis conditions for obtaining the optimum DH using Alcalase® were – temperature of 55oC, hydrolysis time of 163 min, pH of substrate at 9.45 and an enzyme concentration of 2.0%. The generated model showed a quadratic fit with experimental data. Proximate analyses revealed that silver catfish frame contained 25.02% protein, 68.21% fat and 7.08% ash. While silver catfish frame hydrolysate powder contained 65.05% protein, 32.92% fat and 0.86% ash. The protein recovery in silver catfish frame hydrolysate was as high as 71.6%.
    Matched MeSH terms: Catfishes
  11. Amiza, M.A., Ow, Y.W., Faazaz, A.L.
    MyJurnal
    The physicochemical properties of silver catfish frame hydrolysate powder at three different degree of hydrolysis, DH43%, DH 55% and DH 68% were studied. The hydrolysates powder were obtained by hydrolysis using Alcalase®, centrifugation and spray drying of the supernatant. The study found that preparation of these hydrolysates affected the protein, ash and fat content as well as amino acid composition. As for essential amino acids, their values were generally considered as adequate as compared to the suggested essential amino acids profile of FAO/WHO. The results showed that SFHs were rich in lysine and glutamate. Hydrolysate at DH 68% exhibited better peptide solubility and water holding capacity. As degree of hydrolysis increased, emulsifying capacity and foaming capacity of the hydrolysate decreased. It was also found that the lightness in hydrolysate powder decreased with increase in degree of hydrolysis. This study shows that silver catfish frame hydrolysate has good solubility, good foaming properties and light colour profile, thus having high potential as food ingredient.
    Matched MeSH terms: Catfishes
  12. Asmah, R., Siti Sumaiyah, S.A., Nurul, S.R.
    MyJurnal
    Omega-3 fatty acids have been shown to reduce the risk of chronic diseases like cardiovascular disease and cancer as well as promote brain development among infants and children. This study was carried out to compare total protein, fat and omega-3 fatty acids content of raw and pressurized fish of P. pangasius (yellowtail catfish) and H. macrura (long tail shad). The fish was cooked using pressure cooker for six minute to be pressurized. The protein content was determined by using Kjedahl method while total fat was determined using solvent extraction using chloroform and methanol. Fatty acid methyl esters (FAME) were prepared by a direct transesterification method, and quantified by gas chromatography using external standard. Results showed that marine fish H. macrura (long tail shad) had higher content (p < 0.05) of protein (18.30 ± 0.040 g/100 g), fat (10.965 ± 1.610 g/100 g), EPA (11.83 ± 0.02 g/100 g) and DHA (5.96 ± 0.31 g/100 g) compared to freshwater fish P. pangasius (yellowtail catfish). The protein content of pressurized fish was higher compare to raw fish, but there was no difference in total fat and omega-3 fatty acids content between raw and pressurized of freshwater fish P. pangasius and marine fish, H. macrura. In conclusion, marine fish are better source of protein, fat and omega-3 content, while pressurized fish shown to have comparable amount of protein, fat and omega-3 fatty acids content with raw fish. The result obtained assist the consumers to prepare a healthy menu in order to retain the protein and omega-3 fatty acids content of fish through healthy way of cooking.
    Matched MeSH terms: Catfishes
  13. Azizah Endut, Fathurrahman Lananan, Ahmad Jusoh, Wan Norsani Wan Nik, Nora'aini Ali
    MyJurnal
    The current and escalating extent of soil degradation, water scarcity and environmental concern
    plaguing agricultural productivity, demands re-assessing the direction of food production. Aquaponics
    is a concept relatively new to modern food production methods and can contribute to food security.
    This study was conducted to establish sustainable aquaculture systems that maximize benefits and
    minimize the accumulation of detrimental compounds and other types of negative impacts on both
    natural and social environments. This study carried out at an average inflow rate of 1.28 m/day to
    evaluate the operation of the aquaponics recirculation system (ARS) on nutrients removal and growth
    and yield of African catfish as well as water spinach. A special design of ARS was used to provide
    nitrification of fishery wastewater, where the combination of sands and gravels in hydroponics trough,
    providing both surfaces for biofuel development and cultivation area for plants. Removal efficiencies
    of 5-day biochemical oxygen demand (BOD5), total suspended solids (TSS), total ammonia nitrogen
    (TAN), nitrite-nitrogen (NO2-N), nitrate-nitrogen (NO3-N), and orthophosphate (PO4
    3-
    ) were 82%, 89%,
    93%, 94%, 81%, and 80%, respectively. The feed conversion ratio (FCR) and specific growth rate
    (SGR) of African catfish were 1.08 and 3.34% day-1
    , respectively. The average water spinach
    production was 3.56 kg per m2
    . This study showed that ARS is a method of producing crop along with
    a healthy protein source and among the best alternatives for achieving economic and environmental
    sustainability.
    Matched MeSH terms: Catfishes
  14. Aznan AS, Lee KL, Low CF, Iberahim NA, Wan Ibrahim WN, Musa N, et al.
    Fish Shellfish Immunol, 2018 Jul;78:338-345.
    PMID: 29684603 DOI: 10.1016/j.fsi.2018.04.033
    Outbreaks of edwardsiellosis have severe impact on the aquaculture production of African catfish Clarias gariepinus. In this study, feed supplemented with apple mangrove Sonneratia caseolaris extract was evaluated for its protective effect against Edwardsiella tarda infection in African catfish. Results showed an increase in growth performance and higher survival rate in the treatment groups in a dose dependent manner. Haematological analyses showed an increase in white blood cell count in the treatment groups. Histopathological analysis revealed degenerative changes and regeneration of liver tissue architecture in both the control and treatment groups. However, the presence of inflammatory cells was found exclusively in the kidney of T3 treatment group that was supplemented with the highest dose of extract at 3.17 mg/ml, which inferred the activation of immune response in the fish. Contrast to the deteriorative alteration observed in the kidney of the control group due to E. tarda infection, treatment group exhibited tissue regeneration and well-defined kidney tissue architecture at 3 dpi. Taken together, these results demonstrated that supplementation with the methanol extract of S. caseolaris possesses protective effect in African catfish against the infection of E. tarda.
    Matched MeSH terms: Catfishes/growth & development; Catfishes/immunology*
  15. Bahari IB, Noor FM, Daud NM
    Mutat Res, 1994 Aug;313(1):1-5.
    PMID: 7519306
    An in vivo study on the effects of both physical (gamma-radiation) and chemical (mitomycin C) genotoxic agents was carried out with the catfish species, Clarias gariepinus. The fish were either exposed to gamma-radiation at doses of 0-9 Gy or injected intraperitoneally with mitomycin C at concentrations of 0-2 mg/kg. Micronucleated erythrocytes were sampled from 0 to 60 days post treatment. Data obtained showed a time-dependent response of the induction of micronucleated erythrocytes with both genotoxic agents. A linear dose-dependent increase was observed 2-4 days after treatment. These data show the importance of sampling time in the micronucleus assay with Clarias gariepinus.
    Matched MeSH terms: Catfishes
  16. Baki MA, Shojib MFH, Sehrin S, Chakraborty S, Choudhury TR, Bristy MS, et al.
    Environ Geochem Health, 2020 Feb;42(2):531-543.
    PMID: 31376046 DOI: 10.1007/s10653-019-00386-4
    This study aimed to assess the effects of major ecotoxic heavy metals accumulated in the Buriganga and Turag River systems on the liver, kidney, intestine, and muscle of common edible fish species Puntius ticto, Heteropneustes fossilis, and Channa punctatus and determine the associated health risks. K was the predominant and reported as a major element. A large concentration of Zn was detected in diverse organs of the three edible fishes compared with other metals. Overall, trace metal analysis indicated that all organs (especially the liver and kidney) were under extreme threat because the maximum permissible limit set by different international health organizations was exceeded. The target hazard quotient and target cancer risk due to the trace metal content were the largest for P. ticto. Thus, excessive intake of P. ticto from the rivers Buriganga and Turag could result in chronic risks associated with long-term exposure to contaminants. Histopathological investigations revealed the first detectable indicators of infection and findings of long-term injury in cells, tissues, and organs. Histopathological changes in various tissue structures of fish functioned as key pointers of connection to pollutants, and definite infections and lesion types were established based on biotic pointers of toxic/carcinogenic effects. The analysis of histopathological alterations is a controlling integrative device used to assess pollutants in the environment.
    Matched MeSH terms: Catfishes
  17. Bashir FA, Shuhaimi-Othman M, Mazlan AG
    J Environ Public Health, 2012;2012:352309.
    PMID: 22046193 DOI: 10.1155/2012/352309
    This study is focused on evaluating the trace metal levels in water and tissues of two commercial fish species Arius thalassinus and Pennahia anea that were collected from Kapar and Mersing coastal waters. The concentrations of Fe, Zn, Al, As, Cd and Pb in these coastal waters and muscle, liver and gills tissues of the fishes were quantified. The relationship among the metal concentrations and the height and weight of the two species were also examined. Generally, the iron has the highest concentrations in both water and the fish species. However, Cd in both coastal waters showed high levels exceeding the international standards. The metal level concentration in the sample fishes are in the descending order livers > gills > muscles. A positive association between the trace metal concentrations and weight and length of the sample fishes was investigated. Fortunately the level of these metal concentrations in fish has not exceeded the permitted level of Malaysian and international standards.
    Matched MeSH terms: Catfishes
  18. Brunton LA, Desbois AP, Garza M, Wieland B, Mohan CV, Häsler B, et al.
    Sci Total Environ, 2019 Oct 15;687:1344-1356.
    PMID: 31412468 DOI: 10.1016/j.scitotenv.2019.06.134
    Aquaculture systems are highly complex, dynamic and interconnected systems influenced by environmental, biological, cultural, socio-economic and human behavioural factors. Intensification of aquaculture production is likely to drive indiscriminate use of antibiotics to treat or prevent disease and increase productivity, often to compensate for management and husbandry deficiencies. Surveillance or monitoring of antibiotic usage (ABU) and antibiotic resistance (ABR) is often lacking or absent. Consequently, there are knowledge gaps for the risk of ABR emergence and human exposure to ABR in these systems and the wider environment. The aim of this study was to use a systems-thinking approach to map two aquaculture systems in Vietnam - striped catfish and white-leg shrimp - to identify hotspots for emergence and selection of resistance, and human exposure to antibiotics and antibiotic-resistant bacteria. System mapping was conducted by stakeholders at an interdisciplinary workshop in Hanoi, Vietnam during January 2018, and the maps generated were refined until consensus. Thereafter, literature was reviewed to complement and cross-reference information and to validate the final maps. The maps and component interactions with the environment revealed the grow-out phase, where juveniles are cultured to harvest size, to be a key hotspot for emergence of ABR in both systems due to direct and indirect ABU, exposure to water contaminated with antibiotics and antibiotic-resistant bacteria, and duration of this stage. The pathways for human exposure to antibiotics and ABR were characterised as: occupational (on-farm and at different handling points along the value chain), through consumption (bacterial contamination and residues) and by environmental routes. By using systems thinking and mapping by stakeholders to identify hotspots we demonstrate the applicability of an integrated, interdisciplinary approach to characterising ABU in aquaculture. This work provides a foundation to quantify risks at different points, understand interactions between components, and identify stakeholders who can lead and implement change.
    Matched MeSH terms: Catfishes
  19. Budiati T, Rusul G, Wan-Abdullah WN, Chuah LO, Ahmad R, Thong KL
    J Food Prot, 2016 Apr;79(4):659-65.
    PMID: 27052872 DOI: 10.4315/0362-028X.JFP-15-372
    A total of 43 Salmonella enterica isolates belonging to different serovars (Salmonella Albany, Salmonella Agona, Salmonella Corvallis, Salmonella Stanley, Salmonella Typhimurium, Salmonella Mikawasima, and Salmonella Bovismorbificans) were isolated from catfish (Clarias gariepinus) and tilapia (Tilapia mossambica) obtained from nine wet markets and eight ponds in Penang, Malaysia. Thirteen, 19, and 11 isolates were isolated from 9 of 32 catfish, 14 of 32 tilapia, and 11 of 44 water samples, respectively. Fish reared in ponds were fed chicken offal, spoiled eggs, and commercial fish feed. The genetic relatedness of these Salmonella isolates was determined by random amplified polymorphic DNA PCR (RAPD-PCR) using primer OPC2, repetitive extragenic palindromic PCR (REP-PCR), and pulsed-field gel electrophoresis (PFGE). Composite analysis of the RAPD-PCR, REP-PCR, and PFGE results showed that the Salmonella serovars could be differentiated into six clusters and 15 singletons. RAPD-PCR differentiated the Salmonella isolates into 11 clusters and 10 singletons, while REP-PCR differentiated them into 4 clusters and 1 singleton. PFGE differentiated the Salmonella isolates into seven clusters and seven singletons. The close genetic relationship of Salmonella isolates from catfish or tilapia obtained from different ponds, irrespective of the type of feed given, may be caused by several factors, such as the quality of the water, density of fish, and size of ponds.
    Matched MeSH terms: Catfishes/microbiology*
  20. Chang CC, Saad B, Surif M, Ahmad MN, Md Shakaff AY
    Sensors (Basel), 2008 Jun 01;8(6):3665-3677.
    PMID: 27879900
    A disposable screen-printed e-tongue based on sensor array and pattern recognition that is suitable for the assessment of water quality in fish tanks is described. The characteristics of sensors fabricated using two kinds of sensing materials, namely (i) lipids (referred to as Type 1), and (ii) alternative electroactive materials comprising liquid ion-exchangers and macrocyclic compounds (Type 2) were evaluated for their performance stability, sensitivity and reproducibility. The Type 2 e-tongue was found to have better sensing performance in terms of sensitivity and reproducibility and was thus used for application studies. By using a pattern recognition tool i.e. principal component analysis (PCA), the e-tongue was able to discriminate the changes in the water quality in tilapia and catfish tanks monitored over eight days. E-tongues coupled with partial least squares (PLS) was used for the quantitative analysis of nitrate and ammonium ions in catfish tank water and good agreement were found with the ion-chromatography method (relative error, ±1.04- 4.10 %).
    Matched MeSH terms: Catfishes
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