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Sea water shaping the freshwater biota: Hidden diversity and biogeographic history in the Paracanthocobitis zonalternans species complex (Teleostei: Nemacheilidae) in western Southeast Asia

Bohlen J, Dvořák T, Šlechta V, Šlechtová V

Mol Phylogenet Evol, 2020 07;148:106806.
PMID: 32247884 DOI: 10.1016/j.ympev.2020.106806

Western Southeast Asia is hosting one of the world's most diverse faunas, and one of the reasons for this huge diversity is the complex geologic past of the area, increasing the frequency of isolation and expansion events over evolutionary time scale. As an example case, the present study reveals the phylogeny and biogeographic history of the Paracanthocobitis zonalternans species complex, small benthic freshwater fish (Teleostei: Nemacheilidae) that are commonly occurring across western Southeast Asia (from central Myanmar through western and southern Thailand to northern Malaysia). The group is particularly interesting since it occurs in three biogeographic subdivisions (Indian, Indochinese, Malay/Sundaic) and across all of the major biogeographic barriers in the region. Basing on mitochondrial and nuclear sequence data of 93 samples from about 50 localities we found six major clades, most with exclusive geographic distribution. Divergence time dated the origin of the P. zonalternans species complex to early Miocene (17.8 MYA) and a biogeographic analysis identified the Tenasserim region as the ancestral region. From this region the fish spread during periods of lowered global sea level, particularly during late Miocene (11-8 MYA) northwards into all Burmese river basins and southwards into south Thailand and northern Malaysia. Besides lowered global sea level periods, local stream capture events allowed the complex to expand, e.g. into the Mae Klong basin. Strong fragmentations during periods with elevated sea level during the Pliocene and Pleistocene repeatedly restricted populations to refuges and shaped the observed major lineages. Our results document a higher diversity within the P. zonalternans species complex than formerly believed and a strong impact of global sea level on its evolutionary history. Low sea levels promoted dispersal and elevated sea levels fragmentation events. A very similar impact of sea level changes can be expected in all stationary fauna (freshwater and terrestrial) in all non-mountainous coastal regions worldwide.

Matched MeSH terms: Fishes
Fulltext Cell entry of a host-targeting protein of oomycetes requires gp96

Trusch F, Loebach L, Wawra S, Durward E, Wuensch A, Iberahim NA, et al.

Nat Commun, 2018 06 14;9(1):2347.
PMID: 29904064 DOI: 10.1038/s41467-018-04796-3

The animal-pathogenic oomycete Saprolegnia parasitica causes serious losses in aquaculture by infecting and killing freshwater fish. Like plant-pathogenic oomycetes, S. parasitica employs similar infection structures and secretes effector proteins that translocate into host cells to manipulate the host. Here, we show that the host-targeting protein SpHtp3 enters fish cells in a pathogen-independent manner. This uptake process is guided by a gp96-like receptor and can be inhibited by supramolecular tweezers. The C-terminus of SpHtp3 (containing the amino acid sequence YKARK), and not the N-terminal RxLR motif, is responsible for the uptake into host cells. Following translocation, SpHtp3 is released from vesicles into the cytoplasm by another host-targeting protein where it degrades nucleic acids. The effector translocation mechanism described here, is potentially also relevant for other pathogen-host interactions as gp96 is found in both animals and plants.

Matched MeSH terms: Fishes/parasitology*
Fulltext Heavy Metals Uptake by Asian Swamp Eel, Monopterus albus from Paddy Fields of Kelantan, Peninsular Malaysia: Preliminary Study

Yin SA, Ismail A, Zulkifli SZ

Trop Life Sci Res, 2012 Dec;23(2):27-38.
PMID: 24575231 MyJurnal

Swamp eel, Monopterus albus is one of the common fish in paddy fields, thus it is suitable to be a bio-monitor for heavy metals pollution studies in paddy fields. This study was conducted to assess heavy metals levels in swamp eels collected from paddy fields in Kelantan, Malaysia. The results showed zinc [Zn (86.40 μg/g dry weight)] was the highest accumulated metal in the kidney, liver, bone, gill, muscle and skin. Among the selected organs, gill had the highest concentrations of lead (Pb), cadmium (Cd) and nickel (Ni) whereas muscle showed the lowest total metal accumulation of Zn, Pb, copper (Cu), Cd and Ni. Based on the Malaysian Food Regulation, the levels of Zn and Cu in edible parts (muscle and skin) were within the safety limits. However, Cd, Pb and Ni exceeded the permissible limits. By comparing with the maximum level intake (MLI), Pb, Ni and Cd in edible parts can still be consumed. This investigation indicated that M. albus from paddy fields of Kelantan are safe for human consumption with little precaution.

Matched MeSH terms: Fishes
Fulltext Recognize fish as food in policy discourse and development funding

Bennett A, Basurto X, Virdin J, Lin X, Betances SJ, Smith MD, et al.

Ambio, 2021 May;50(5):981-989.
PMID: 33454882 DOI: 10.1007/s13280-020-01451-4

The international development community is off-track from meeting targets for alleviating global malnutrition. Meanwhile, there is growing consensus across scientific disciplines that fish plays a crucial role in food and nutrition security. However, this 'fish as food' perspective has yet to translate into policy and development funding priorities. We argue that the traditional framing of fish as a natural resource emphasizes economic development and biodiversity conservation objectives, whereas situating fish within a food systems perspective can lead to innovative policies and investments that promote nutrition-sensitive and socially equitable capture fisheries and aquaculture. This paper highlights four pillars of research needs and policy directions toward this end. Ultimately, recognizing and working to enhance the role of fish in alleviating hunger and malnutrition can provide an additional long-term development incentive, beyond revenue generation and biodiversity conservation, for governments, international development organizations, and society more broadly to invest in the sustainability of capture fisheries and aquaculture.

Matched MeSH terms: Fishes
Mapping the world's free-flowing rivers

Grill G, Lehner B, Thieme M, Geenen B, Tickner D, Antonelli F, et al.

Nature, 2019 05;569(7755):215-221.
PMID: 31068722 DOI: 10.1038/s41586-019-1111-9

Free-flowing rivers (FFRs) support diverse, complex and dynamic ecosystems globally, providing important societal and economic services. Infrastructure development threatens the ecosystem processes, biodiversity and services that these rivers support. Here we assess the connectivity status of 12 million kilometres of rivers globally and identify those that remain free-flowing in their entire length. Only 37 per cent of rivers longer than 1,000 kilometres remain free-flowing over their entire length and 23 per cent flow uninterrupted to the ocean. Very long FFRs are largely restricted to remote regions of the Arctic and of the Amazon and Congo basins. In densely populated areas only few very long rivers remain free-flowing, such as the Irrawaddy and Salween. Dams and reservoirs and their up- and downstream propagation of fragmentation and flow regulation are the leading contributors to the loss of river connectivity. By applying a new method to quantify riverine connectivity and map FFRs, we provide a foundation for concerted global and national strategies to maintain or restore them.

Matched MeSH terms: Fishes
Fulltext The effects of chilling and freezing to the crude fat, ash and moisture content of Channa striata (Bloch, 1793) and Clarias batrachus (Linnaeus, 1758)

Sharir Aizat Kamaruddin, Muhammad Huzaifah Jaafar, Muhammad Ariff Zakariya, Muhammad Fayyadh Fadzilan, Aliff Iqbal Ahmad Nadzri, Khairul Naim Abd.Aziz, et al.

Journal of Academia Universiti Teknologi MARA Negeri Sembilan, 2020;8(2):39-47.
MyJurnal

This research is conducted to investigate the effects of cubed and crushed ice on the crude fat, ash, and
moisture content of Channa striata and Clarias batrachus. The samples were chilled to four and seven days in the Polyvinyl chloride plastic (PVC) boxes filled with cubed ice and crushed ice. Before the determination of crude, ash, and moisture content, the samples were frozen for 1 week. Statistical
analyses were carried out to indicate the significant difference in the effects of treatment days and
physical characteristics of ice towards the crude fat, ash, and moisture content. The result concludes that crushed ice is more superior to cubed ice for all experiments. The outcomes of this research benefit many parties specially to cubed ice suppliers to support aquaculture industries and the socio-economic sectors in Malaysia.

Matched MeSH terms: Fishes
Feeding ecology and food preferences of Carcinoscorpius rotundicauda collected from the Pahang nesting grounds

Akbar John B, Kamaruzzaman B, Jalal K, Zaleha K

Sains Malaysiana, 2012;41:855-861.

The first time report on the feeding ecology and food preference of mangrove horseshoe crab Carcinoscorpius rotundicauda (Latreille 1802) at their nesting grounds along the Pahang coast is given. Monthly sampling was carried out between March 2010 and February 2011 covering both monsoonal (March to October) and non-monsoonal (November to February) seasons. Major macrobenthic gut contents (bivalves, gastropods, crustaceans, polychaetes and miscellaneous food items including plant materials) were identified using microscopic examination. An electivity index (E1) was calculated for the frequent food items observed in the gut region of C. rotundicauda during monsoon and non-monsoon seasons. The EI was negative for crustaceans and positive for all the other food items including bivalves, gastropods, polychaetes and miscellaneous food items (which include insects, amphipods, Isopods, larval and juvenile stages of fishes, foraminifera and other Annelid worms). It is interesting to note that C. rotundicauda prefered less number of bivalves than polychaetes during non monsoon seasons but it was the reverse during monsoonal period. Male crabs intensely preyed on gastropods and female prefers polychaete worms during the peak mating/nesting season (June - August 2010). Seasonal variations in food composition showed that mollusks formed the main item especially gastropods. Unidentified organic matters in the gut content analysis of C. rotundicauda showed high preference towards plant materials. Gastro Somatic Index (GaSI) analysis showed that the feeding intensity of male crabs was higher during non-monsoon period while it was higher during monsoonal period in female crabs. In conclusion, the feeding ecology of mangrove horseshoe crabs were more similar to its closer and distant conspecifics. However, it was postulated that the higher preference of polychaete worms by the female C. rotundicauda during the peak mating season indicated its role in regulating the nesting behaviour.

Matched MeSH terms: Fishes
Chemical and Chemometric Methods for Halal Authentication of Gelatin: An Overview

Hassan N, Ahmad T, Zain NM

J Food Sci, 2018 Dec;83(12):2903-2911.
PMID: 30440088 DOI: 10.1111/1750-3841.14370

The issue of food authenticity has become a concern among religious adherents, particularly Muslims, due to the possible presence of nonhalal ingredients in foods as well as other commercial products. One of the nonhalal ingredients that commonly found in food and pharmaceutical products is gelatin which extracted from porcine source. Bovine and fish gelatin are also becoming the main commercial sources of gelatin. However, unclear information and labeling regarding the actual sources of gelatin in food and pharmaceutical products have become the main concern in halal authenticity issue since porcine consumption is prohibited for Muslims. Hence, numerous analytical methods involving chemical and chemometric analysis have been developed to identify the sources of gelatin. Chemical analysis techniques such as biochemical, chromatography, electrophoretic, and spectroscopic are usually combined with chemometric and mathematical methods such as principal component analysis, cluster, discriminant, and Fourier transform analysis for the gelatin classification. A sample result from Fourier transform infrared spectroscopy analysis, which combines Fourier transform and spectroscopic technique, is included in this paper. This paper presents an overview of chemical and chemometric methods involved in identification of different types of gelatin, which is important for halal authentication purposes.

Matched MeSH terms: Fishes
Phototrophic purple bacteria as feed supplement on the growth, feed utilization and body compositions of Malaysian mahseer, tor tambroides juveniles

Nor Hafizah Zakaria, Mohammad Mustafizur Rahman, Ahmed Jalal Khan Chowdhury, Zaima Azira Zainal Abidin

Sains Malaysiana, 2016;45:135-140.

The application of microbial techniques in aquaculture has been playing a vital role to increase the production yield by improving the nutritional values of the targeted fish. Phototrophic purple bacteria as single cell protein (SCP) appears to be a promising substitution for protein rich supplement for aquaculture feeds making them a promising growth enhancer in aquaculture industry. Two species of phototrophic purple bacteria, Marichromatium sp. and Rhodopseudomonas sp. were used in the commercial diet to compare the growth, survival rate and feed utilizationon for Tor tambroides juvenile. Purple bacteria were isolated from mangrove sediment and fish tank and mass cultured using 112 synthetic media under anaerobic light condition. Bacterial cells were included in the diets by mixing the fresh biomass with the crushed commercial pellet, re-pelleted and air-dried. The experimental diets were fed to the fingerlings twice per day for 10 weeks to satiation level. The results showed that there were trends of increased growth, better survival rate and improved feed conversion ratio when fed with diet 1 (Marichromatium sp.) compared with other diets. There was significant difference (p<0.05) between the sampling days. The specific growth rate and weight gain of the fish fed with diet 1 were 0.49 % and 4.92 g, respectively, compared to 0.42% and 4.11 g from the control. This study suggested that purple bacteria could be used in feed formulation as a supplement to promote growth and survival of freshwater fishes in Malaysia.

Matched MeSH terms: Fishes
Universal mini COI barcode for the identification of fish species in processed products

Sultana S, Ali ME, Hossain MAM, Asing, Naquiah N, Zaidul ISM

Food Res Int, 2018 03;105:19-28.
PMID: 29433207 DOI: 10.1016/j.foodres.2017.10.065

Species substitution, the use of a low value fish in place of a high value fish, is the biggest problem in international trade and the leading cause of fraud in the fisheries arena sector. Current DNA barcoding systems have partly solved this problem but also failed in many instances to amplify PCR targets from highly processed products because of the degradation of a longer barcode marker (~650bp). In the present study, a novel mini barcode marker (295bp) was developed to discriminate fish species in raw and processed states forms. The barcode primers were cross-tested against 33 fish species and 15 other animal species and found to be universal for all the tested fish varieties. When 20 commercial fish products of five different categories were screened, all commercial fish sample yielded positive bands for the novel fish barcode. PCR product was sequenced to retrieve the species IDs that reflected 55% (11/20) of Malaysian fish products were mislabeled.

Matched MeSH terms: Fishes/classification*
Dietary exposure to heterocyclic amines in high-temperature cooked meat and fish in Malaysia

Jahurul MH, Jinap S, Ang SJ, Abdul-Hamid A, Hajeb P, Lioe HN, et al.

Food Addit Contam Part A Chem Anal Control Expo Risk Assess, 2010 Aug;27(8):1060-71.
PMID: 20589547 DOI: 10.1080/19440041003801190

The intake of heterocyclic amines is influenced by the amount and type of meat and fish ingested, frequency of consumption, cooking methods, cooking temperature, and duration of cooking. In this study, the dietary intake of heterocyclic amines in Malaysia and their main sources were investigated. Forty-two samples of meat and fish were analysed by high-performance liquid chromatography with photodiode array detector to determine the concentration of the six predominant heterocyclic amines, namely: 2-amino-3-methylimidazo[4,5-f] quinoline (IQ), 2-amino-3,4-dimethylimidazo[4,5-f] quinoline(MeIQ), 2-amino-3,8-dimethylimidazo[4,5-f] quinoxaline (MeIQx), 2-amino-3,4,8-trimethylimidazo[4,5-f] quinoxaline (4,8-DiMeIQx), 2-amino-3,7,8-trimethylimidazo[4,5-f] quinoxaline (7,8-DiMeIQx), and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). Dietary intake data were obtained using a food-frequency questionnaire when interviewing 600 Malaysian respondents. The level of total heterocyclic amines in food samples studies ranged from not detected to 38.7 ng g(-1). The average daily intake level of heterocyclic amine was 553.7 ng per capita day(-1). The intake of PhIP was the highest, followed by MeIQx and MeIQ. The results reveal that fried and grilled chicken were the major dietary source of heterocyclic amines in Malaysia. However, the heterocyclic amine intake by the Malaysian population was lower than those reported from other regions.

Matched MeSH terms: Fishes
Gene mutation effect of aqueous and methanol extracts of salted fish from pulau pinang, malaysia towards v79 lung fibroblast cells

AHMAD ROHI GHAZALI, RASYIDAH MOHAMAD HALIM, NOR FADILAH RAJAB, NOORAIN RAMLI, ROZAINI ABDULLAH, FIRDAUS KAMARULZAMAN, et al.

Sains Malaysiana, 2013;42:1599-1603.

Salted fish is a locally processed raw food which is used in everyday cooking among Malaysians. Previous studies suggested that salted fish intake was a risk of nasopharyngeal cancer. Hence, this study was carried out to evaluate gene mutation effects through the induction of mutagenic effect of aqueous and methanol extracts of salted fish from Balik Pulau, Pulau Pinang, Malaysia. Balik Pulau was chosen for sampling purpose due to its popularity as a commercial centre for local raw fisheries in Malaysia. Evaluation of mutagenic effect was carried out by hprt Gene Mutation Assay towards V79 lung fibroblast cells. It was found that the aqueous and methanol extracts of salted fish were not cytotoxic towards V79 lung fibroblast cells. It was also found that the extracts of salted fish from Balik Pulau were not mutagenic towards hprt gene of V79 lung fibroblast cells as the mutation frequency of the extracts did not exceed 3 times of the value for negative control mutation frequency. In conclusion, both aqueous and methanol extracts of salted fish from Balik Pulau did not have gene mutation effect towards hprt gene in vitro. However, other toxicological profile could be assessed to determine the mechanism of toxicity of salted fish.

Matched MeSH terms: Fishes
Condition factors of two archerfish species from Johor coastal waters, Malaysia

K.D. Simon, A.G. Mazlan, Z.C. Cob

Sains Malaysiana, 2013;42:1115-1119.

Archerfishes Toxotes chatareus (Hamilton 1822) and Toxotes jaculatrix (Pallas 1767) inhabits mangrove estuaries, which are a critically important habitat as a spawning and nursery ground as well as the full life cycle of many fish species. In order to manage and conserve resilience fish species like the archerfish, we need to know some basic parameters about their biology and life history. Our research has focused on understanding the annual condition factors of these two species of archerfishes, as a complimentary to other several biological aspects of this two species that have been published. Our results indicated that, both species are in good conditions as the mean values of condition factors (Fulton condition factor K and Relative condition factor Kn) are greater than values of unity (K, Kn >1). The average K and Kn values of T. chatareus and T. jaculatrix were lowest in November and highest in September, indicates the spawning and recovery condition of the fishes. Condition factors measure overall fish population health that can be used by fisheries biologists and resource managers in proper management of fish resources in Malaysia waters.

Matched MeSH terms: Fishes
Population structure of the Southeast Asian river catfish Mystus nemurus

Usmani S, Tan SG, Siraj SS, Yusoff K

Anim. Genet., 2003 Dec;34(6):462-4.
PMID: 14687079

A total of 143 microsatellites were isolated from Mystus nemurus using a 5' anchored polymerase chain reaction technique or the random amplified hybridization microsatellite method, the first set of microsatellite markers developed for the Southeast Asian river catfish. Twenty polymorphic microsatellite loci were used as markers for population characterization of M. nemurus from six different geographical locations in Malaysia (Perak, Kedah, Johor, UPM, Sarawak and Terengganu). The number of alleles per locus ranged from 2 to 11 with 6.3 as the average number of alleles per locus. Characterization of the populations showed relatively high levels of genetic variation compared with previous studies using allozyme markers. The highest genetic similarity was found between Perak and Kedah, while the highest genetic distance was found between Terengganu and Kedah. The majority of clustering was in accordance with geographical locations and the histories of the populations. Microsatellite analysis indicated that the Sarawak population might be genetically closer to the Peninsular Malaysian populations than has been previously shown by other molecular marker studies.

Matched MeSH terms: Fishes/classification; Fishes/genetics*
Comparison of four antibiotics with indigenous marine Bacillus spp. in controlling pathogenic bacteria from shrimp and Artemia

Banerjee S, Devaraja TN, Shariff M, Yusoff FM

J Fish Dis, 2007 Jul;30(7):383-9.
PMID: 17584435

Use of antibiotics for the control of bacterial diseases in shrimp culture has caused several adverse impacts to the industry. This has resulted in the search for alternative environment friendly approaches to overcome bacterial infections. This study was conducted to investigate the use of beneficial bacteria as an alternative to antibiotics. Ten pathogenic bacterial species isolated from shrimp, Penaeus monodon, and Artemia cysts were tested for susceptibility to indigenous marine Bacillus subtilis AB65, Bacillus pumilus AB58, Bacillus licheniformis AB69 and compared with oxytetracycline, chloramphenicol, gentamicin and bacitracin, which are common antibiotics used in Asian aquaculture. The Bacillus spp. were isolated from the local marine environment for bioremediation use in shrimp hatcheries and were proven to reduce total ammonium nitrogen. The pathogenic bacterial isolates were 90% susceptible to B. subtilis AB65, 70% susceptible to B. pumilus AB58 and B. licheniformis AB69 and 100% susceptible to oxytetracycline, chloramphenicol and gentamicin but only 40% to bacitracin. Two representative isolates of the vibrio group, Vibrio alginolyticus VaM11 and Vibrio parahaemolyticus VpM1, when tested for competitive exclusion by a common broth method using the marine Bacillus spp., showed decreased viable counts from 10(8) to 10(2) cfu mL(-1). The results suggest that the action of the marine bacteria appears to be significant in protecting the host shrimp against pathogenic bacteria. In addition to the alternative use of antibiotics, the selected marine bacteria had additional bioremediation properties of reducing ammonia.

Matched MeSH terms: Fishes
Effects of varying dietary carbohydrate levels on growth performance, body composition and liver histology of Malaysian mahseer fingerlings (Tor tambroides)

Ishak SD, Kamarudin MS, Ramezani-Fard E, Saad CR, Yusof YA

J Environ Biol, 2016 07;37(4 Spec No):755-64.
PMID: 28779735

We investigated the effects of four iso-nitrogenous (40% crude protein) and iso-caloric (17.6 kJ g(-1)) diets with different dietary carbohydrate levels (15%, 20%, 25% and 30%) on the growth performance, feed utilization efficiency, body composition and liver histology of Malaysian mahseer (Tor tambroides) fingerlings in a 10-week feeding trial. Fish (initial weight of 0.8?0.1 g; initial total length 4.2?0.1 cm) were fed twice daily at 4% body mass. Dietary carbohydrate level had significant effects (P<0.05) on weight gain, SGR (specific growth rate), FCR (feed conversion rate), PER (protein efficiency rate), survival percentage and all nutrient retention values (PRV, LRV, CRV, ERV). Protein, carbohydrate and gross energy composition of the fish body were also significantly differed (P<0.05) among treatments. Liver histology showed mild hepatic steatosis and hypertrophy for fishes receiving a higher dietary carbohydrate inclusion. In general, treatments with 20% and 25% dietary carbohydrate levels produced better growth results compared to the rest of the treatments. Using a second-order polynomial regression analysis model, the optimal dietary carbohydrate level of 23.4% was estimated for mahseer fingerlings. ?

Matched MeSH terms: Fishes/growth & development*
Fulltext Randomized Controlled Trial on the Effect of Channa striatus Extract on Measurement of the Uterus, Pulsatility Index, Resistive Index of Uterine Artery and Superficial Skin Wound Artery in Post Lower Segment Caesarean Section Women

Abu Bakar MR, Abdul Kadir A, Abdul Wahab SZ, Abdul Karim AH, Nik Hussain NH, Mohd Noor N, et al.

PLoS One, 2015;10(7):e0133514.
PMID: 26222158 DOI: 10.1371/journal.pone.0133514

AIM: To compare the mean of anteroposterior (AP) measurements of the uterus in longitudinal and oblique transverse planes, and the pulsatility index (PI) and resistive index (RI) of the uterine artery and superficial skin wound artery between patients taking Channa striatus and placebo.
BACKGROUND: Channa striatus, also known as haruan, is a fresh water snakehead fish consumed in many parts of Southeast Asia. Channa striatus is also normally consumed by women postpartum to promote wound healing as well as to reduce post-operative pain.
METHODOLOGY: This study is a randomised, double blind, placebo-controlled study conducted in women after Lower Segment Caesarean Section (LSCS). Subjects were randomised to either a Channa striatus or a placebo group and were given a daily dosage of 500 mg of Channa striatus extract or 500 mg maltodextrin, respectively, for six weeks post LSCS. The anteroposterior measurements of the uterus in the longitudinal and oblique transverse planes, and the pulsatility index (PI) and resistive index (RI) of the uterine and superficial skin wound arteries were assessed using pelvic Gray-scale ultrasound and Doppler ultrasound at baseline (Day 3) and at two weeks, four weeks and six weeks post-operatively.
RESULTS: Sixty-six subjects were randomised into the study with 33 in the Channa striatus group and 33 in the placebo group. No significant differences were detected in terms of the pulsatility index (PI) and the resistive index (RI) of the uterine and superficial skin wound arteries between the Channa striatus and placebo groups. However, in the Channa striatus group, the AP measurements of the uterus on the longitudinal and oblique transverse planes were significantly lower compared to the placebo group (p<0.05 and p<0.001, respectively).
CONCLUSION: Daily intake of Channa striatus extract results in marked differences compared to placebo in terms of uterine involution and recovery in women post LSCS.
TRIAL REGISTRATION: www.isrctn.com 11960786.

Matched MeSH terms: Fishes/physiology
Pyridine-4-carbaldehyde azine

Shanmuga Sundara Raj S, Fun HK, Zhang J, Xiong RG, You XZ

Acta Crystallogr C, 2000 Jun 1;56(Pt 6):E274-5.
PMID: 15263128

In the crystal structure of the title compound, C(12)H(10)N(4), the pyridine ring makes a dihedral angle of 1.12 (9) degrees with the mean plane of the complete almost planar and crystallographically centrosymmetric molecule. There are stacks of parallel molecules along the a-axis direction, with alternate stacks having a herring-bone arrangement relative to each other and an interplanar spacing of 3.551 A.

Matched MeSH terms: Fishes
Fulltext Dissemination of streptococcal pyrogenic exotoxin G (spegg) with an IS-like element in fish isolates of Streptococcus dysgalactiae

Abdelsalam M, Chen SC, Yoshida T

FEMS Microbiol Lett, 2010 Aug 1;309(1):105-13.
PMID: 20528946 DOI: 10.1111/j.1574-6968.2010.02024.x

The Lancefield group C alpha-hemolytic Streptococcus dysgalactiae ssp. dysgalactiae (GCSD) causes systemic granulomatous inflammatory disease and high mortality rates in infected fish. Superantigen and streptolysin S genes are the most important virulence factors contributing to an invasive streptococcal infection. PCR amplification revealed that all strains isolated from moribund fish harbored the streptolysin S structural gene (sagA). GCSD fish isolates were PCR negative for emm, speA, speB, speC, speM, smeZ, and ssa. However, the size of the streptococcal pyrogenic exotoxin G (spegg) locus, a superantigen, in positive S. dysgalactiae fish and pig strains was variable. The ORF of the spegg locus of 26 GCSD fish strains and one GCSD pig strain was inserted with IS981SC. Interestingly, the ORF of the spegg locus of two fish strains of GCSD collected in Malaysia was inserted with an IS981SC-IS1161 hybrid IS element. The hybrid IS element was found in all of the GCSD fish isolates and one GCSD pig through PCR screening. Although no insertion sequence (IS) was detected in the spegg locus of S. dysgalactiae ssp. equisimilis (GCSE) strains, a five-nucleotide deletion mutation was detected in the ORF of the spegg locus of one GCSE strain at the supposed site of IS981SC insertion, resulting in a frameshift mutation.

Matched MeSH terms: Fishes
Fulltext Molecular quantitation and characterization of Vibrio cholerae from different seafood obtained from wetmarket and supermarket

Vengadesh, L., Son, R., Yoke-Kqueen, C.

International Food Research Journal, 2012;19(1):45-50.
MyJurnal

Vibrio cholerae still represents a significant threat to human health worldwide despite the advances in hygiene, consumer knowledge, food treatment and food processing. In Malaysia, statistics in year 2009 have shown that among the food and water borne diseases, food poisoning has the highest incidence rate of 36.17 per 100,000 populations and with a mortality rate of 0.01 per 100,000 populations. In this study, 22 seafood samples comprising of fish, squid, crustacean and mollusks purchased from wet market and supermarket were analyzed. The Most Probable Number (MPN) and real time PCR was used to enumerate the Vibrio cholerae in seafood sample. The results showed that MPN-real time PCR of the samples from wet market had a maximum of >1100 MPN/g compare to 93 MPN/g enumerated from the MPN plate. The MPN-real time PCR in the samples from supermarket indicated 290 MPN/g as compared to 240 MPN/g enumerated from the MPN plate. The standard curves showed that there was a good linear correlation between the Ct values. The minimum level of detection of Vibrio cholerae standard DNA at targeted gene was 3 x 10-5 ng/μl.

Matched MeSH terms: Fishes

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