Displaying publications 1 - 20 of 44 in total

Abstract:
Sort:
  1. Ivorra T, Martínez-Sánchez A, Rojo S
    Acta Trop, 2022 Feb;226:106233.
    PMID: 34808117 DOI: 10.1016/j.actatropica.2021.106233
    The larval development of Chrysomya albiceps and Lucilia sericata is a well-known and valuable tool for estimating the postmortem interval (PMI). The third instar larvae of Ch. albiceps are facultative predators of the larvae of other necrophagous dipterans, and this behaviour is undoubtedly an important factor to consider. Both species are found together during human autopsies in Spain and other parts of Europe, where they cohabit, so a precise knowledge of their larval coexistence data is essential. The aim of this study is to investigate their coexistence and the intraguild predation of Ch. albiceps on L. sericata under experimental conditions and in real case reports. To analyse intra- and interspecific competition, four densities were used under controlled abiotic conditions [25ºC, 60-70% RH and 12:12 (D:N)]. The experimental data were compared with data corresponding to their coexistence in natural conditions, so annual activity and forensic case reports for both species were studied. The results indicate that the mortality of immature specimens in both species, the preimaginal developmental time, and adult size were affected by competition. In natural conditions, adult coexistence during the spring-summer period was confirmed. The simultaneity of the two species colonising human corpses is frequent in the studied area, with Ch. albiceps being the dominant species, and L. sericata the relevant species for estimating the minimum postmortem interval.
    Matched MeSH terms: Forensic Sciences
  2. Tan, Soon Guan
    MyJurnal
    In various biological studies, for example those in population genetics, conservation biology, forensic science, gene mapping, breed, strain and population characterization and identification, marker assisted selection and the identification of cryptic species complexes, codominant genetic markers play important roles. The information that can be gained from them are far superior than those from dominant markers like random amplified polymorphic DNA (RAPD), amplified fragment length polymorphisms (AFLP), direct amplification of length polymorphisms (DALP) and randomly amplified microsatellites (RAM) or inter simple sequence repeats (ISSR).
    Matched MeSH terms: Forensic Sciences
  3. Chong, Lee Kim
    MyJurnal
    Animal species identification is one of the important fields in forensic science. Unlike human forensics which makes use of DNA fingerprinting techniques to identify individuals of the same species - humans, animal forensic species identification is much more complicated as it involves the ability to identify and distinguish between hundreds to thousands of species when the material evidence is only a trace of animal tissue without the presence of any visual physical morphology. It is even more difficult when the specimen is an unknown and no reference material is available. Animal species identification is not only important for the prevention of wildlife crimes for the purpose of wildlife protection and conservation but it is also becoming more and more significant in food safety issues especially for the meat industry. Owing to the demand and the necessity of providing such services for regulation and enforcement in the context of environmental protection, food safety and biosafety, the Department of Chemistry (DOC)
    Malaysia has initiated the use of DNA techniques employing the most widely used genetic markers as part of its scientific solution for animal species identification.
    Matched MeSH terms: Forensic Sciences
  4. Nur Atiqah Zaharulli
    ESTEEM Academic Journal, 2020;16(2):88-95.
    MyJurnal
    Questioned document examination becomes a great interest and one of the broad fields in forensic science. It involves the analysis of ink, handwriting and signature examination, paper’s physical structure analysis and the ageing of a document. Ink analysis in forensic document examination is a challenging process. Questioned documents examiners are dealing with unknown source of ink and minute sample size. Ink extraction needs to be done before the ink analysis. 17 gel pen ink samples were chosen in this study. Solubility test has been done to determine the degree of solubility of ink in a variety of organic solvents. Extraction solvent optimization is a process to evaluate the efficiency of organic solvents to extract ink samples. Ethanoic acid showed the ability to dissolve most of the ink samples and displayed maximum absorbance of UV-Vis spectra.
    Matched MeSH terms: Forensic Sciences
  5. Ong SQ, Ahmad H, Tan EH
    Environ Entomol, 2018 12 07;47(6):1582-1585.
    PMID: 30165432 DOI: 10.1093/ee/nvy127
    Megaselia scalaris (Loew) (Diptera: Phoridae) provides great evidential value in estimating the postmortem interval (PMI) compared with other dipterans due to its common occurrence on human corpses both indoors and in concealed environments. Studies have focused on the effect of temperature, larval diet, and photoperiod on the development of the species; however, knowledge of M. scalaris development at different moisture levels is insufficient. This study aimed to investigate the effects of substrate moisture on the larval development time, pupal recovery, pupal weight, adult emergence, and adult head width of M. scalaris. The larvae were reared in five replicates on substrates with six moisture levels ranging from 50 to 90%. Larvae and puparia were sampled daily, and the collection time, number, and weight were recorded, measured, and then compared using multivariate analysis of variance with a post hoc least significant difference test. Larvae developed most quickly (3.75 ± 0.04 d) at 50% substrate moisture; the larvae were able to survive in extremely wet substrates (90% moisture), but the development time was significantly longer (6.48 ± 0.19 d). Moisture greatly influenced the pupation rate and adult emergence but showed a weak effect on the pupae weight and adult head width. Due to the significance of moisture on the development of M. scalaris, PMI estimation using M. scalaris with cadavers of different moisture content must be carefully conducted to avoid inaccuracy.
    Matched MeSH terms: Forensic Sciences
  6. Jayaprakash PT
    Forensic Sci Int, 2013 Sep 10;231(1-3):403.e1-16.
    PMID: 23849815 DOI: 10.1016/j.forsciint.2013.05.028
    Uniqueness being unprovable, it has recently been argued that individualization in forensic science is irrelevant and, probability, as applied for DNA profiles, should be applied for all identifications. Critiques against uniqueness have omitted physical matching, a realistic and tangible individualization that supports uniqueness. Describing case examples illustrating pattern matches including physical matching, it is indicated that individualizations are practically relevant for forensic science as they establish facts on a definitive basis providing firm leads benefitting criminal investigation. As a tenet of forensic identification, uniqueness forms a fundamental paradigm relevant for individualization. Evidence on the indeterministic and stochastic causal pathways of characteristics in patterns available in the related fields of science sufficiently supports the proposition of uniqueness. Characteristics involved in physical matching and matching achieved in patterned evidence existing in the state of nature are not events amenable for counting; instead these are ensemble of visible units occupying the entire pattern area stretching the probability of re-occurrence of a verisimilitude pattern into infinity offering epistemic support to uniqueness. Observational methods are as respectable as instrumental or statistical methods since they are capable of generating results that are tangible and obviously valid as in physical matching. Applying the probabilistic interpretation used for DNA profiles to the other patterns would be unbefitting since these two are disparate, the causal pathways of the events, the loci, in the manipulated DNA profiles being determinable. While uniqueness enables individualizations, it does not vouch for eliminating errors. Instead of dismissing uniqueness and individualization, accepting errors as human or system failures and seeking remedial measures would benefit forensic science practice and criminal investigation.
    Matched MeSH terms: Forensic Sciences/methods*
  7. Yin SH, Kuppuswamy R
    Forensic Sci Int, 2009 Jan 10;183(1-3):50-3.
    PMID: 19041202 DOI: 10.1016/j.forsciint.2008.10.009
    Chemical etching, which is the most sensitive method to recover obliterated serial numbers on metal surfaces, has been practised quite successfully in forensic science laboratories all over the world. A large number of etchants suitable for particular metal surfaces based on empirical studies is available in the literature. This article reviews the sensitivity and efficacy of some popular etchants for recovering obliterated marks on medium carbon steel (0.31% C with ferrite-pearlite microstructure) used in automobile parts. The experiments involved engraving these carbon steel plates with some alphanumeric characters using a computer controlled machine "Gravograph" and erasing them to several depths below the bottom of their engraving depth. Seven metallographic reagents of which most of them were copper containing compounds were chosen for etching. The erased plates were etched with every one of these etchants using swabbing method. The results have revealed that Fry's reagent comprising cupric chloride 90 g, hydrochloric acid 120 mL and water 100mL provided the necessary contrast and was concluded to be the most sensitive. The same reagent was recommended by earlier workers for revealing strain lines in steel surfaces. Earlier, another reagent containing 5 g copper sulphate, 60 mL water, 30 mL (conc.) ammonium hydroxide, and 60 mL (conc.) hydrochloric acid was proved to be more sensitive to restore erased marks on low carbon steel (0.1% C with ferrite-pearlite structure) [M.A.M. Zaili, R. Kuppuswamy, H. Harun, Restoration of engraved marks on steel surfaces by etching technique, Forensic Sci. Int. 171 (2007) 27-32]. Thus the sensitivity of the etching reagent on steel surfaces appeared to be dependent on the content of carbon in the steel.
    Matched MeSH terms: Forensic Sciences
  8. Lee WC, Khoo BE, Abdullah AFL
    Forensic Sci Int, 2016 06;263:1-9.
    PMID: 27061146 DOI: 10.1016/j.forsciint.2016.03.046
    Evidence in crime scenes available in the form of biological stains which cannot be visualized during naked eye examination can be detected by imaging their fluorescence using a combination of excitation lights and suitable filters. These combinations selectively allow the passage of fluorescence light emitted from the targeted stains. However, interference from the fluorescence generated by many of the surface materials bearing the stains often renders it difficult to visualize the stains during forensic photography. This report describes the use of background correction algorithm (BCA) to enhance the visibility of seminal stain, a biological evidence that fluoresces. While earlier reports described the use of narrow band-pass filters for other fluorescing evidences, here, we utilize BCA to enhance images captured using commonly available colour filters, yellow, orange and red. Mean-based contrast adjustment was incorporated into BCA to adjust the background brightness for achieving similarity of images' background appearance, a crucial step for ensuring success while implementing BCA. Experiment results demonstrated the effectiveness of our proposed colour filters' approach using the improved BCA in enhancing the visibility of seminal stains in varying dilutions on selected surfaces.
    Matched MeSH terms: Forensic Sciences/methods
  9. Mohd Noor MS, Khoo LS, Zamaliana Alias WZ, Hasmi AH, Ibrahim MA, Mahmood MS
    Forensic Sci Int, 2017 Sep;278:410.e1-410.e9.
    PMID: 28698062 DOI: 10.1016/j.forsciint.2017.05.014
    The first ever mass identification operation of skeletal remains conducted for the clandestine graves in Malaysia consisted of 165 individuals unearthed from 28 human trafficking transit camps located in Wang Kelian, along the Thai-Malaysia border. A DVI response was triggered in which expert teams comprising of pathologists, anthropologists, odontologists, radiologists and DNA experts were gathered at the identified operation centre. The Department of Forensic Medicine, Hospital Sultanah Bahiyah, Alor Star, Kedah, located approximately 75km away from Wang Kelian, was temporarily converted into a victim identification centre (VIC) as it is the nearest available forensic facility to the mass grave site. The mortuary operation was conducted over a period of 3 months from June to September 2015, and was divided into two phases; phase 1 involving the postmortem examination of the remains of 116 suspected individuals and for phase 2 the remains of 49 suspected individuals. The fact that the graves were of unknown individuals afforded the mass identification operation a sufficient duration of 2 weeks as preparatory phase enabling procedurals and daily victim identification workflow to be established, and the setting up of a temporary body storage for the designated mortuary. The temporary body storage has proven to be a significant factor in enabling the successful conclusion of the VIC operation to the final phase of temporary controlled burials. Recognition from two international observers, Mr. Andréas Patiño Umaña, from the International Committee of Red Cross (ICRC) and Prof. Noel Woodford from Victoria Institute of Forensic Medicine (VIFM) had proven the mortuary operation was in compliance to the international quality and standards. The overall victim identification and mortuary operation identified a number of significant challenges, in particular the management of commingled human remains as well as the compilation of postmortem data in the absence of antemortem data for future reconciliation. CF Index in this DVI operation is 9%, indicating the primary identifications in this operation were effective. Limitations and further improvements of the mass identification operation will be discussed. This paper details the planning, preparations and management of the mass identification operation on the exhumed human remains which was also a forensic humanitarian service for the dead with dignity and respect.
    Matched MeSH terms: Forensic Sciences/organization & administration*
  10. Jahanirad M, Anuar NB, Wahab AWA
    Forensic Sci Int, 2017 Mar;272:111-126.
    PMID: 28129583 DOI: 10.1016/j.forsciint.2017.01.010
    The VoIP services provide fertile ground for criminal activity, thus identifying the transmitting computer devices from recorded VoIP call may help the forensic investigator to reveal useful information. It also proves the authenticity of the call recording submitted to the court as evidence. This paper extended the previous study on the use of recorded VoIP call for blind source computer device identification. Although initial results were promising but theoretical reasoning for this is yet to be found. The study suggested computing entropy of mel-frequency cepstrum coefficients (entropy-MFCC) from near-silent segments as an intrinsic feature set that captures the device response function due to the tolerances in the electronic components of individual computer devices. By applying the supervised learning techniques of naïve Bayesian, linear logistic regression, neural networks and support vector machines to the entropy-MFCC features, state-of-the-art identification accuracy of near 99.9% has been achieved on different sets of computer devices for both call recording and microphone recording scenarios. Furthermore, unsupervised learning techniques, including simple k-means, expectation-maximization and density-based spatial clustering of applications with noise (DBSCAN) provided promising results for call recording dataset by assigning the majority of instances to their correct clusters.
    Matched MeSH terms: Forensic Sciences
  11. Chung NE, Castilani A, Tierra WE, Beh P, Mahmood MS
    Forensic Sci Int, 2017 Sep;278:367-373.
    PMID: 28806635 DOI: 10.1016/j.forsciint.2017.07.030
    On December 1st, 2014, the sinking of Oryong 501 occurred in the Bering Sea off the east coast of Russia. A total of 60 crew members, including 35 Indonesians, 13 Filipinos, 11 South Koreans and 1 Russian inspector were on board out of which only seven survived. Through an international rescue operation, the dead bodies of 27 were found and the remaining 26 crew are still missing. After transferring the dead bodies to the Busan Harbor in South Korea, the operation to identify the deceased began involving DVI teams from three countries: Korea, Indonesia and the Philippines. When a deep sea fishing boat sinks, it is very difficult to obtain antemortem data of the crew who had been on board for a long time. This is especially so if the crews are multinational. Further, the accuracy of the antemortem data provided by the families may be questionable, and the provided data is often not standardized. Despite the fact that the antemortem data were received in different formats, the identification process for the bodies of the 27 crew from the Oryong sinking was quickly completed through the cooperation among the three DVI teams. This case is an excellent example of how efficiently a DVI operation can be conducted in the Asia Pacific region. Issues raised during this operation should enable even better preparation for similar events in the future.
    Matched MeSH terms: Forensic Sciences/organization & administration*
  12. Khoo LS, Hasmi AH, Ibrahim MA, Mahmood MS
    Forensic Sci Med Pathol, 2020 09;16(3):463-470.
    PMID: 32519316 DOI: 10.1007/s12024-020-00269-6
    The emergence of a novel human coronavirus, SARS-CoV-2, causing severe respiratory tract infections in humans, is affecting all countries of the world and has become a global health concern. Since the virus was first identified in December 2019, the number of deaths have been propagating exponentially, causing countries across the world, including Malaysia, to increase emergency measures to combat the virus. Due to the fact that the COVID-19 pandemic does not discriminate its victims, it is of paramount importance to construct a plan for management of the dead for all suspected or confirmed COVID-19 cases, including the unidentified deceased, as an essential portion of the humanitarian forensic action approach. This document provides an overview on ways to maximize the local collective capacity from various government agencies to manage the dead based on the prevailing regulations and legislation in the country, in preparation for possible large scale deaths from this pandemic. The National Institute of Forensic Medicine Malaysia has improvised procedures and guidelines for management of the dead within the existing regulations in order to achieve a balance between medicolegal requirements and the safety of personnel managing the bodies of the deceased with suspected or confirmed COVID-19 infection; at the site of death, during transport, during postmortem procedures, storage and preparation before and during burial or cremation as well as environmental cleaning and disinfection, involving various agencies in the country. A form of temporary controlled burial is as an option to allow the reinvestigation of a decedent to help formally identify victims of the pandemic such as undocumented migrants or refugees who were previously not identified. Due to the different legal requirements and mortality rates between countries, there is no one-size-fits-all approach to the management of the dead. Whenever possible, every opportunity and assistance must be given to families to mourn their loved ones, even in times of crisis or an outbreak, in order to sustain an appropriate level of dignity and respect.
    Matched MeSH terms: Forensic Sciences/organization & administration*
  13. Azman AR, Mahat NA, Abdul Wahab R, Abdul Razak FI, Hamzah HH
    Int J Mol Sci, 2018 May 25;19(6).
    PMID: 29799469 DOI: 10.3390/ijms19061576
    Waterways are popular locations for the disposition of criminal evidence because the recovery of latent fingerprints from such evidence is difficult. Currently, small particle reagent is a method often used to visualize latent fingerprints containing carcinogenic and hazardous compounds. This study proposes an eco-friendly, safranin-tinted Candida rugosa lipase (triacylglycerol ester hydrolysis EC 3.1.1.3) with functionalized carbon nanotubes (CRL-MWCNTS/GA/SAF) as an alternative reagent to the small particle reagent. The CRL-MWCNTS/GA/SAF reagent was compared with the small particle reagent to visualize groomed, full fingerprints deposited on stainless steel knives which were immersed in a natural outdoor pond for 30 days. The quality of visualized fingerprints using the new reagent was similar (modified-Centre for Applied Science and Technology grade: 4; p > 0.05) to small particle reagent, even after 15 days of immersion. Despite the slight decrease in quality of visualized fingerprints using the CRL-MWCNTS/GA/SAF on the last three immersion periods, the fingerprints remained forensically identifiable (modified-Centre for Applied Science and Technology grade: 3). The possible chemical interactions that enabled successful visualization is also discussed. Thus, this novel reagent may provide a relatively greener alternative for the visualization of latent fingerprints on immersed non-porous objects.
    Matched MeSH terms: Forensic Sciences/methods*
  14. Syamsa RA, Omar B, Ahmad FM, Hidayatulfathi O, Shahrom AW
    J Forensic Leg Med, 2017 Jan;45:41-46.
    PMID: 27997861 DOI: 10.1016/j.jflm.2016.12.002
    Forensic entomology refers to the science of collection and analysis of insect evidence in order to determine the minimum time period since death. This study aimed to investigate the occurrence of forensically important flies on 34 human remains referred to Universiti Kebangsaan Malaysia Medical Centre over a period of three years. Entomological specimens were collected at the death scenes and/or during autopsies. Live specimens were reared into adults while preserved specimens were processed for species identification. Five families, seven genera and nine species of flies were identified from human remains. The results of the study showed Chrysomya megacephala (Calliphoridae) maggots occurred on corpses with the highest frequency (70.6%), followed by Ch. rufifacies (Calliphoridae) (44.1%), sarcophagid fly (Sarcophagidae) (38.2%), Synthesiomya nudiseta (Muscidae) (20.6%), Megaselia scalaris (Phoridae) (14.7%), Lucilia cuprina (Calliphoridae) (5.9%), Ch. nigripes (Calliphoridae) (5.9%), Eristalis spp. (Syrphidae) (5.9%) and Hydrotaea spinigera (Muscidae) (2.9%). The greatest fly diversity occurred on remains recovered indoors (eight species) compared to outdoors (three species). Whilst, single and double infestations were common for both indoor and outdoor cases, multiple infestation of up to six species was observed in one of the indoor cases. Although large numbers of fly species were found on human remains, the predominant species were still those of Chrysomya, while S. nudiseta was found only on human remains recovered from indoors. The present study provides additional knowledge in the context of Malaysian forensic entomology and the distribution of forensically important flies which is of relevance to forensic science.
    Matched MeSH terms: Forensic Sciences
  15. Cugati N, Kumaresan R, Srinivasan B, Karthikeyan P
    J Forensic Dent Sci, 2015 Sep-Dec;7(3):227-31.
    PMID: 26816464 DOI: 10.4103/0975-1475.172445
    BACKGROUND: Age estimation is of prime importance in forensic science and clinical dentistry. Age estimation based on teeth development is one reliable approach. Many radiographic methods are proposed on the Western population for estimating dental age, and a similar assessment was found to be inadequate in Malaysian population. Hence, this study aims at formulating a regression model for dental age estimation in Malaysian children population using Cameriere's method.
    MATERIALS AND METHODS: Orthopantomographs of 421 Malaysian children aged between 5 and 16 years involving all the three ethnic origins were digitalized and analyzed using Cameriere's method of age estimation. The subjects' age was modeled as a function of the morphological variables, gender (g), ethnicity, sum of normalized open apices (s), number of tooth with completed root formation (N0) and the first-order interaction between s and N0.
    RESULTS: The variables that contributed significantly to the fit were included in the regression model, yielding the following formula: Age = 11.368-0.345g + 0.553No -1.096s - 0.380s.No, where g is a variable, 1 for males and 2 for females. The equation explained 87.1% of total deviance.
    CONCLUSION: The results obtained insist on reframing the original Cameriere's formula to suit the population of the nation specifically. Further studies are to be conducted to evaluate the applicability of this formula on a larger sample size.
    KEYWORDS: Age estimation; Cameriere's method; dental age; forensic odontology
    Matched MeSH terms: Forensic Sciences
  16. Cheng N, Lee GK, Yap BS, Lee LT, Tan SK, Tan KP
    J Forensic Sci, 2005 Jan;50(1):177-84.
    PMID: 15831016
    This paper investigated the class characteristics in English handwriting of the Chinese, Malays and Indians in Singapore, many of whom learned their native language as a second language. One hundred and fifty-four handwriting exemplars were collected and features such as letter designs, pen-lifts, letter spacing and embellishments were studied. A number of characteristic features peculiar to the individual racial group were identified, which confirmed the impact of their native language writing systems on English handwriting.
    Matched MeSH terms: Forensic Sciences/methods
  17. Nordin FA, Bominathan UR, Abdullah AFL, Chang KH
    J Forensic Sci, 2020 Jan;65(1):11-25.
    PMID: 31373699 DOI: 10.1111/1556-4029.14142
    Wherever an impact mark is found, either on the surface or on the recovered projectile, it is important for forensic investigators to extract useful information in solving shooting-related cases. This article reviews a collection of works on examination of impact marks upon striking of projectiles on inanimate objects, emphasizing on the retrievable information from a shooting scene and their forensic significance in shooting event reconstruction. Literature suggested that impact marks on target surfaces and the degree of deformation on striking projectiles vary according to different combinations of ammunition and surface materials. It was noted that conditions in real-case scenarios further differed unpredictably in comparison with controlled studies, where forensic investigation should be treated as case-specific basis. Furthermore, the way forensic science is researched and applied operationally has to be reconsidered to reduce the gap via translational approach for more effective use of forensic evidence.
    Matched MeSH terms: Forensic Sciences
  18. Ahmad A, Ahmad AH, Dieng H, Satho T, Ahmad H, Aziz AT, et al.
    J Med Entomol, 2011 Nov;48(6):1236-46.
    PMID: 22238885
    There is accumulating evidence that criminals wrap dead bodies in an attempt to conceal evidence. To anticipate the forensic implications of this phenomenon, we examined whether flies that are naturally associated with cadavers exhibit a delay in attendance or differ in species composition and abundance patterns because of the presence of wrapping material. Wrapped and exposed carcasses of dead monkeys placed in an oil plantation in Kedah, Malaysia, were visited over 50 d. On daily visits to each of the six carcasses, visiting adult flies were sampled using hand nets. Flies of 12 families were encountered. Calliphoridae (Chrysomya rufifacies Macquart and C. megacephala (F.) was the most prevalent family, followed by Sphaeroceridae. Some families tended to be more abundant in WRCs (i.e., Calliphoridae, Muscidae, and Phoridae), whereas others (i.e., Piophilidae, Sepsidae, and Psychodidae) were more prevalent in exposed carcasses. Wrapping delayed the arrival of all fly species encountered, with delays varying from 1 to 13 d depending on species. Wrapping did not affect species composition of flies, but prolong the occurrence of some species. The results of the current study emphasize the need to take into consideration the presence of a wrap when estimating postmortem interval.
    Matched MeSH terms: Forensic Sciences
  19. Raja Muhammad Zuha, Balkhis Bashuri, Supriyani Mustamin, Baharudin Omar, Nazni Wasi Ahmad
    MyJurnal
    In forensic entomology practice, it is more common to use raw animal tissue to breed dipteran larvae and it often brings unpleasant odour in the laboratory. Few studies suggested the use of synthetic diets, mainly agar-based media, as alternatives to animal tissue but it is rarely being practiced in forensic entomology laboratory. The present study observed the growth of a forensically important fly, Megaselia scalaris (Loew) on raw cow’s liver, nutrient agar, casein agar and cow’s liver agar. A total of 100 M. scalaris eggs were transferred each into the different media and placed in an incubator at 30°C in a continuous dark condition. Data on length and developmental period were collected by randomly sampling three of the largest larvae from each rearing media, twice a day at 0900 and 1500 hours until pupariation. M. scalaris larvae reared on raw cow’s liver recorded the highest mean length (4.23 ± 1.96 mm) followed by cow’s liver agar (3.79 ± 1.62 mm), casein agar (3.14 ± 1.16 mm) and nutrient agar (3.09 ± 1.11 mm). Larval length in raw liver and liver agar were significantly different from those in nutrient and casein agar (p < 0.05). Larvae bred in liver agar and raw liver recorded the shortest larval duration before entering the post-feeding stage (89 hours), followed by nutrient agar (119 hours) and casein agar (184 hours). Total developmental time from oviposition until adult emergence for M. scalaris in liver agar and raw liver was approximately 163 hours. All puparia in nutrient agar and casein agar failed to hatch. This research highlighted the potential use of cow’s liver agar as an alternative diet of raw liver to culture M. scalaris in laboratory.
    Matched MeSH terms: Forensic Sciences
Filters
Contact Us

Please provide feedback to Administrator (afdal@afpm.org.my)

External Links