Displaying publications 1 - 20 of 127 in total

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  1. Loke P, Lim YAL
    Cell Host Microbe, 2016 10 12;20(4):417-419.
    PMID: 27736641 DOI: 10.1016/j.chom.2016.09.016
    Gut commensals profoundly affect host immunity and intestinal homeostasis, but the impact of commensal eukaryotic protozoans is poorly understood. In a recent Cell paper, Chudnovskiy et al. (2016) identify a commensal protozoan, Tritrichomonas musculis, that can enhance anti-bacterial defenses, but at the cost of increasing intestinal inflammation.
    Matched MeSH terms: Intestines/immunology*
  2. Chung ELT, Reduan MFH, Nordin ML, Abdullah FFJ, Zairi NHM, Rajdi NZIM, et al.
    J Adv Vet Anim Res, 2020 Dec;7(4):692-697.
    PMID: 33409314 DOI: 10.5455/javar.2020.g469
    Objective: This case report aims to discuss the veterinary approach taken to control a case of aspergillosis outbreak on a duck farm.

    Materials and Methods: A broiler duck farm with a population of 900 Muscovy ducks was having a complaint of a 5% mortality rate in their 3-week-old ducklings. Upon presentation, 10% of the ducks appeared to be listless, dyspneic, ruffled feathers, and cyanotic. Postmortem examination of the dead birds was conducted. The collected samples were subjected to isolation and identification of the associated Aspergillus fumigatus under the microscope using the scotch tape method.

    Results: Postmortem examination revealed whitish to creamy caseous nodules in the lungs, thoracic air sacs, gizzard, proventriculus, and intestines. Granuloma lesions and infiltration of inflammatory cells were observed in the lung and liver tissues. As for therapeutic management, all ducks were treated with copper sulfate, erythromycin, and multivitamins as the fungicide, antibiotic, and supplement, respectively, via drinking water.

    Conclusion: There is no effective treatment for Aspergillosis as the spores are difficult to destroy completely. Nonetheless, the disease can be controlled and prevented effectively with proper farm sanitation and providing a suitable feed storage environment to inhibit the growth of this opportunistic fungus.

    Matched MeSH terms: Intestines
  3. Ng GH, Teoh LY, Teh MS, Jamaris S, See MH
    J Surg Case Rep, 2020 Oct;2020(10):rjaa328.
    PMID: 33093939 DOI: 10.1093/jscr/rjaa328
    Shiitake mushroom is a common ingredient in East Asian cuisines. Food processing/preparation can cause the mushroom to be soft and slimy, leading to accidental swallowing. Due to its high insoluble fibre content, it remains the same size and shape in the intestinal tract. We present two cases of small bowel obstruction caused by shiitake mushroom requiring surgical intervention. Preoperative imaging showed dilated small bowel with a suspicious mass in the ileum. However, the exact cause was unclear. For both cases, exploratory laparotomy and enterotomy were then performed and undigested shiitake mushroom was found. Both patients recovered well from the surgery.
    Matched MeSH terms: Intestines
  4. Tan RZ, Darwin FL, Anuar Zainun K
    Malays J Pathol, 2020 Dec;42(3):477-481.
    PMID: 33361732
    Gastrointestinal pathology leading to the death in paediatric age group is uncommon. The diseases that encountered were mostly intestinal obstruction, peritonitis and gastrointestinal bleeding. Due to the severe symptoms, most of the patients presented to hospital in time and were treated appropriately. However, with the presence of contributing factors, certain gastrointestinal pathology can progress rapidly leading to the death. We report a rare case of intestinal volvulus in a 3 years old girl where the deceased presented with one day short history of vomiting before her demise. The contributing factors were bronchopneumonia sepsis and underlying intestinal malrotation identified via post-mortem examination.
    Matched MeSH terms: Intestines/abnormalities*
  5. Liu Y, Yu Q, Shu YL, Zhao JH, Fang JY, Wu HL
    J. Helminthol., 2019 Jul 12;94:e59.
    PMID: 31296272 DOI: 10.1017/S0022149X19000518
    We identified and characterized a new cosmocercid nematode species, Cosmocercoides wuyiensis n. sp., through microscopic examination and sequencing of the partial small ribosomal RNA gene (18S rDNA), internal transcribed spacer (ITS) and mitochondrial cytochrome c oxidase subunit 1 (COI) genes. The new species was isolated from the intestine of the Asiatic frog Amolops wuyiensis Liu and Hu, 1975 captured from four localities of the Anhui province in south-east China. Among the 25 recorded species of the Cosmocercoides genus, the morphology of C. wuyiensis n. sp. is closest to that of C. kiliwai and C. malayensis, which were isolated from various Mexican frog and Malaysian lizard species, respectively. However, C. wuyiensis n. sp. displayed several distinguishing features, such as small size of the male body, two spicules of unequal lengths in the male, small gubernaculum, pre-, ad- and post-cloacal caudal rosette papillae in the ratio of 18-24:2:6 and simple papillae in the ratio of 14:multiple:4, circle and number of punctation in each rosette at 1:11-16, sharply conical tail-end and the presence of lateral alae and somatic papillae in both sexes. BLAST and the phylogenetic analyses of the 18S rDNA and ITS sequences indicated that C. wuyiensis n. sp. belonged to the genus Cosmocercoides, while that of the COI gene sequence of C. wuyiensis n. sp. showed 16.36% nucleotide divergence with C. pulcher and 47.99% nucleotide divergence with C. qingtianensis. The morphological and molecular characterization of C. wuyiensis n. sp. provides new taxonomic data for this genus.
    Matched MeSH terms: Intestines/parasitology
  6. Jajere SM, Atsanda NN, Bitrus AA, Hamisu TM, Goni MD
    Vet World, 2018 May;11(5):598-605.
    PMID: 29915497 DOI: 10.14202/vetworld.2018.598-605
    Background and Aim: Bovine tuberculosis (bTB) still remains a major zoonotic bacterial disease affecting livestock and humans worldwide. The disease remains a poorly managed tropical disease in most developing countries of the world; where in addition to productivity losses and significance in international trade, it posed a major public health threat to both humans and animals. A retrospective study was designed to investigate the occurrence of bTB lesions at Bauchi municipal abattoir.

    Materials and Methods: The study utilized abattoir records spanning a period of 10 years (2004-2013). The records indicated that a total of 1,08,638 heads of cattle comprising n = 56,070 males and n = 52,570 females were slaughtered at the municipal abattoir during the study period.

    Result: Of these heads, n = 1230 (1.13%) (95% confidence interval [CI]: 1.07, 1.19) had tuberculous lesions. The annual occurrence during the study period varied significantly (p<0.001) from 0.53% (95% CI: 0.40, 0.67) to 1.87% (95% CI: 1.66, 2.10) in 2010 and 2012, respectively. Females had a significantly higher (p<0.001) prevalence of 2.10% (95% CI: 1.98, 2.23) compared with the males 0.23% (95% CI: 0.19, 0.27). The distribution of suspected gross bTB lesions in different organs showed 11.87% in the lungs, 5.93% in the liver, 1.14% in the heart, and 0.49% accounted for generalized bTB. However, none was observed on the lymph nodes and intestines.

    Conclusion: It can be concluded that bTB persists in Bauchi State with annual variations during the study period. This study highlights the importance of meat inspection as an important tool for detecting the presence of bTB lesions.

    Matched MeSH terms: Intestines
  7. Sinriah B, Poopalachelvam M
    PMID: 7403946
    Matched MeSH terms: Intestines/parasitology
  8. Jin LZ, Ho YW, Abdullah N, Jalaludin S
    Lett Appl Microbiol, 1998 Sep;27(3):183-5.
    PMID: 9750324
    Twelve Lactobacillus strains isolated from chicken intestine were used to investigate acid and bile tolerance in vitro. Ten out of the 12 strains were slightly affected by 0.3% bile salts, showing a delay of growth (d) of 0.6-37.2 min compared with growth in control cultures. Two strains were not affected by the bile salts. Of the 12 strains, seven could be arbitrarily classified as resistant (d < 15 min) and five as tolerant (15 min < d < or = 40 min). Lactobacillus strains from the caecum showed better tolerance to acid than those from the ileum. Generally, the survival of the ileal strains was very low at pH 1.0 and 2.0, and moderate at pH 3.0. In contrast, caecal Lactobacillus strains could survive at pH 1.0 for up to 2 h of incubation; growth was moderate at pH 2.0 and good at pH 3.0 and 4.0.
    Matched MeSH terms: Intestines/microbiology*
  9. Ida Muryany, Ahmad Rohi Ghazali, Nor Fadilah Rajab, Hing HL, Ina-Salwany, Mohd Zamri Saad, et al.
    Sains Malaysiana, 2018;47:2391-2399.
    Bacterial adhesion to host cells is the most important probiotic character. However, the adhesion of probiotic should not
    affect the viability of the host cells. In this study, Lactobacillus plantarum strain L8, Lactobacillus plantarum strain L20
    and Lactobacillus pentosus strain S1 were tested for their cytotoxic effects through MTT assay and their ability to adhere
    and colonize on HT-29 and CCD-18Co intestinal cells as detected microscopically using light microscopy and Scanning
    Electron Microscopy (SEM). No cytotoxicity effects were observed on both intestinal cells following 24 h treatment with
    all Lactobacillus strains. Additionally, all strains demonstrated strong adhesive activity where more than 100 bacteria
    adhered to both intestinal cells although differences in the adhesion scores observed among different strains. The adhesion
    as observed via SEM showed an autoagreggative pattern and adhered as clusters on the surface of both intestinal cells.
    In conclusion, all three Lactobacillus strains are non-cytotoxic to both cells with strong adhesion ability on intestinal
    cells and this study also proved that Malaysian fermented fish are good source of probiotic bacteria.
    Matched MeSH terms: Intestines
  10. Zhou Z, Zhao J, de Cruz CR, Xu H, Wang L, Xu Q
    Fish Physiol Biochem, 2023 Oct;49(5):951-965.
    PMID: 37665506 DOI: 10.1007/s10695-023-01234-0
    The study investigated the alleviated effects of Alpha-ketoglutaric acid (AKG) on the intestinal health of mirror carp (Cyprinus carpio Songpu) caused by soy antigenic protein. The diets were formulated from fishmeal (CON), 50% soybean meal (SBM), the mixture of glycinin and β-conglycinin (11 + 7S) and adding 1% AKG in the 11 + 7S (AKG). Carp (~ 4 g) in triplicate (30 fish per tank) was fed to apparent satiation thrice a day for six weeks. Compared with CON, SBM treatment resulted in significantly poor growth performance (P  0.05). Gene expression of tumor necrosis factor (TNF-α) and interleukin-1 β (IL-1β) in proximal intestines (PI) and distal intestines (DI) were increased (P 
    Matched MeSH terms: Intestines
  11. Mansharan Kaur Chaincel Singh
    MyJurnal
    Amoebiasis is a parasitic infection caused by the
    intestinal protozoan Entamoeba histolytica, most
    prevalent in developing countries. It results in 40,000 to
    100,000 deaths each year from amoebic colitis and extra
    intestinal infections. Amoebic liver abscess (ALA)
    is the most common extra intestinal site of infection
    with an incidence of between 3% and 9% of all cases of
    amoebiasis. Ultrasound which has a sensitivity of more
    than 90% for detecting ALA is highly recommended
    as an initial investigation followed by serological
    demonstration of circulating antibodies specific to
    Entamoeba histolytica.
    Matched MeSH terms: Intestines
  12. Ismail Elshahawy, Ashraf Elgoniemy
    Sains Malaysiana, 2018;47:9-18.
    The present study was designed to study the enteric parasites infection in domestic rabbits in Upper Egypt and its impact on their health. A total of 298 faecal samples were collected from different farms in Upper Egypt. Each faecal sample was screened for parasitic isolation during May 2011- October 2012. Furthermore, The Eimeria species from samples containing isolated and sporulated oocysts were morphologically identified under microscope. Additionally, the Sheather's sugar flotation and Modified Zeihl Neelsen techniques were used to detect the Cryptosporidium species oocyst. According to results of the present study, the overall enteric parasite infections in rabbits was 68.12%, with 5.7% samples were found to be positive for helminths (P. ambiguus) and 49% were found to harbour various species of protozoa (Eimeria spp. and Cryptosporidium oocysts). Influences of age, sex and breed on the prevalence were recorded. Also, there was strong significant seasonal trends in the prevalence of the recovered parasites. Eight species of Eimeria were detected. Concurrent infection with two to eight Eimeria species occurred most frequently. Cryptosporidium oocyst was detected in 45 rabbits (15.1%). To the best of author's knowledge, this the first report of E. piriformis and Cryptosporidium species in rabbits in Egypt. Histopathological changes were indicative of inflammatory reactions brought about by parasitic infection with Eimeria species and its consequent irritating effects on intestine. The current study showed the characteristics of the prevalence of rabbit enteric parasite infection in Egypt and provided relevant 'baseline' data for assessing the effectiveness of future control strategies against these parasites in Egypt.
    Matched MeSH terms: Intestines
  13. Nayak SB, Soumya KV
    Anat Cell Biol, 2020 Sep 30;53(3):372-375.
    PMID: 32647076 DOI: 10.5115/acb.20.082
    Anomalies of the peritoneum and the colon are quite common. Some of these anomalies can disturb the normal digestive and absorptive functions of the intestine and the others might result in formation of volvulus or impede the blood supply of the intestine. We report a rare, combined variation of peritoneum and ascending colon. In a 70-year-old male cadaver, the greater omentum was very small and extended only for about an inch below the transverse colon. From its lower end, a fibrous band extended to the right wall of the upper part of ascending colon. There was a deep constriction on the right wall of the ascending colon at the site of attachment of the fibrous band. The ascending colon was grossly dilated. Further, the ascending colon was mobile and presented a small ascending mesocolon along its left edge. We discuss the possible embryological basis and clinical and surgical relevance of the case.
    Matched MeSH terms: Intestines
  14. Surin J
    PMID: 8525399
    There are few small animals models for filariasis, even more so for onchocerciasis. Therefore it is difficult to test under drug screening conditions large numbers of potentially macrofilaricidal compounds. One way around this difficulty is to use mice infected with Trichinella spiralis which by reason of anatomical location in the host would show some correlation in antinematode activity between the test and target organisms. This study investigated the activity of 16 compounds against the immature larval stage of T. spiralis. All the nine benzimidazole compounds (albendazole, flubendazole, mebendazole, oxfendazole, oxibendazole 780118, 780120, 790163, and 790392) were active, the most potent being oxfendazole. The benzothiazoles (CGP21306, CGP20376, CGP21833 and CGP24588A) also indicated some anti-nematode activity together with 35vr, an imidazopyridine, but not as marked as the benzimidazole group. However, the organic arsenical compounds (Mel Ga and Mel Ni) showed little activity and this was at a rather highly toxic level. The prospects of using the Trichinella-mouse model as a primary screen to test for potential macrofilaricides are discussed.
    Matched MeSH terms: Intestines/parasitology
  15. Rampal L, Oothuman P, Jeffery J, Daud MZ, Shekhar C, Senan P, et al.
    Med J Malaysia, 1983 Jun;38(2):104-7.
    PMID: 6353184
    Bacterial isolates were made from the intestinal tracts ofcarious species of cockroaches (Periplaneta americana, Periplaneta brunnea, Periplaneta australasiae, Neostylopyga rhombifolia, Nauphoeta cinerea) trapped from kitchens and stores (houses and hospital), Shigello, flexneri, Salmonella typhi, Escherichia coli and Salmonella sp. were some of the bacteria isolated and identified.
    Matched MeSH terms: Intestines/microbiology
  16. Khoomsab K, Wannasri S
    Sains Malaysiana, 2017;46:851-858.
    The biological aspects of Channa limbata were studied between November 2013 and October 2014. A total of 346 fish specimens, 185 male and 161 female, were collected from Ta Bo, Huai Yai Wildlife Sanctuary, Phetchabun Province, Thailand. Specimens range from 7.3-17.2 cm in length with body weight 8-31 g; sex ratio between males and females was 1: 0.7. The length (L), weight (W) relationship for mixed sexes was W = 0.2064 L1.85 (R2=0.90). Gonadosomatic indices for males and females were measured monthly and varied from 0.21-0.65% and 1.96-3.74%, respectively. Condition factors for males and females ranged between 0.54 - 2.20 and 0.58 - 2.72, respectively, with fecundity range 956 to 4,652 eggs in females. Fecundity (F) to weight relationship was F = 189.53 W0.59 (R2 = 0.71) and fecundity to length relationship was F = 68.82 L1.15 (R2 = 0.77). The ratio between the intestine length and total length was 1:2, indicating that C. limbata was a carnivorous feeder. Analysis of the stomach contents gave 84% insects and 16% aquatic weed. These results can be applied to conserve efforts to prevent the extinction of C. limbata in protected areas.
    Matched MeSH terms: Intestines
  17. Nurulnadia MY, Koyama J, Uno S, Amano H
    Chemosphere, 2016 Feb;144:185-92.
    PMID: 26363319 DOI: 10.1016/j.chemosphere.2015.08.059
    We evaluated the potential for biomagnification of endocrine disrupting chemicals (EDCs) such as nonylphenol (NP), octylphenol (OP), bisphenol A (BP), and natural estrogens such as estrone (E1) and 17β-estradiol (E2) in a benthic fish, Pleuronectes yokohamae. The assimilation efficiencies (AE) of most EDCs ranged from 88 to 96% suggesting that they were efficiently incorporated and assimilated into P. yokohamae, except for NP (50%). However, the biomagnification factor (BMF) values were <1.0 suggesting that the compounds were not biomagnifying. Additionally, three of the target EDCs were not detected (BP, E1 and E2). Glucuronidation activity towards BP (11.44 ± 2.5 nmol/mg protein/min) and E2 (12.41 ± 3.2 nmol/mg protein/min) was high in the intestine suggesting that EDCs were glucuronidated prior to excretion into bile. Thus, we conclude that biomagnification of dietary EDCs is reduced in P. yokohamae because of effective glucuronidation.
    Matched MeSH terms: Intestines
  18. Karami A, Groman DB, Wilson SP, Ismail P, Neela VK
    Environ Pollut, 2017 Apr;223:466-475.
    PMID: 28129952 DOI: 10.1016/j.envpol.2017.01.047
    There are serious concerns over the adverse impacts of microplastics (MPs) on living organisms. The main objective of this study was to test the effects of MPs on the total length, weight, condition factor (CF), transcriptional level of antioxidant, anti and pro-apoptotic, and neurotransmitter genes, and the histopathology of the gill, liver, brain, kidney, and intestine in the larvae of zebrafish (Danio rerio). Fish were exposed to one of three levels of pristine low-density polyethylene (LDPE) fragments (5, 50, or 500 μg/L) for 10 or 20 days. No significant changes were observed in any of the selected biomarkers across MP concentrations at days 10 or 20. The expression of casp9 (caspase 9, apoptosis-related cysteine protease), casp3a (caspase 3, apoptosis-related cysteine protease a) and cat (catalase), however, were significantly lower in the larvae sampled at day 20 than day 10. We provide evidence that virgin short-term exposure to LDPE fragments has minimal impact on biomarker responses in D. rerio larvae.
    Matched MeSH terms: Intestines/drug effects; Intestines/metabolism
  19. Ooi SK, Lim TY, Lee SH, Nathan S
    Virulence, 2012 Oct 01;3(6):485-96.
    PMID: 23076282 DOI: 10.4161/viru.21808
    The nematode Caenorhabditis elegans is hypersusceptible to Burkholderia pseudomallei infection. However, the virulence mechanisms underlying rapid lethality of C. elegans upon B. pseudomallei infection remain poorly defined. To probe the host-pathogen interaction, we constructed GFP-tagged B. pseudomallei and followed bacterial accumulation within the C. elegans intestinal lumen. Contrary to slow-killing by most bacterial pathogens, B. pseudomallei caused fairly limited intestinal lumen colonization throughout the period of observation. Using grinder-defective mutant worms that allow the entry of intact bacteria also did not result in full intestinal lumen colonization. In addition, we observed a significant decline in C. elegans defecation and pharyngeal pumping rates upon B. pseudomallei infection. The decline in defecation rates ruled out the contribution of defecation to the limited B. pseudomallei colonization. We also demonstrated that the limited intestinal lumen colonization was not attributed to slowed host feeding as bacterial loads did not change significantly when feeding was stimulated by exogenous serotonin. Both these observations confirm that B. pseudomallei is a poor colonizer of the C. elegans intestine. To explore the possibility of toxin-mediated killing, we examined the transcription of the C. elegans ABC transporter gene, pgp-5, upon B. pseudomallei infection of the ppgp-5::gfp reporter strain. Expression of pgp-5 was highly induced, notably in the pharynx and intestine, compared with Escherichia coli-fed worms, suggesting that the host actively thwarted the pathogenic assaults during infection. Collectively, our findings propose that B. pseudomallei specifically and continuously secretes toxins to overcome C. elegans immune responses.
    Matched MeSH terms: Intestines/microbiology
  20. Chai-Hoon, K., Jiun-Horng, S., Shiran, M.S., Son, R., Sabrina, S., Noor Zaleha, A.S., et al.
    MyJurnal
    Caenorhabditis elegans (C. elegans) have been widely used as an infection model for mammalian related pathogens with promising results. The bacterial factors required for virulence in non-mammalian host C. elegans play a role in mammalian systems. Previous reported that Salmonella found in vegetable and poultry meat could be potential health hazards to human. This study evaluated the pathogenicity of various serovars of Salmonella enterica (S. enterica) that recovered from local indigenous vegetables and poultry meat using C. elegans as a simple host model. Almost all S. enterica isolates were capable of colonizing the intestine of C. elegans, causing a significant reduction in the survival of nematodes. The colonization of Salmonella in C. elegans revealed that the ability of S. enterica in killing C. elegans correlates with its accumulation in the intestine to achieve full pathogenicity. Using this model, the virulence mechanisms of opportunistic pathogenic S. enterica were found to be not only relevant for the interactions of the bacteria with C. elegans but also with mammalian hosts including humans. Hence, C. elegans model could provide valuable insight into preliminary factors from the host that contributes to the environmental bacterial pathogenesis scenario.
    Matched MeSH terms: Intestines
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