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New species and records of black flies (Diptera: Simuliidae) from Vinh Phuc Province, Vietnam

Takaoka H, Sofian-Azirun M, Ya'cob Z, Chen CD, Lau KW, Pham HT

Zootaxa, 2014;3838(3):347-66.
PMID: 25081781 DOI: 10.11646/zootaxa.3838.3.6

Four new species of black flies are described, and three others are reported as newly recorded, based on adults reared from pupae, pupae and larvae collected in and near Tam Dao National Park, Vinh Phuc Province, Vietnam. New species include Simulium (Gomphostilbia) hongthaii sp. nov., S. (G.) tamdaoense sp. nov. (both species placed in the asakoae species-group), S. (Simulium) taythienense sp. nov. and S. (S.) xuandai sp. nov. (the two latter species placed in the striatum species-group). Newly recorded species are S. (G.) brinchangense Takaoka, Sofian-Azirun & Hashim, S. (Nevermannia) aureohirtum Brunetti and S. (S.) brevipar Takaoka & Davies. These discoveries increase the number of species of black flies known in Vietnam from 21 to 28.

Matched MeSH terms: Pupa/growth & development
Simulium (Nevermannia) khunklangense, a new species of black fly (Diptera: Simuliidae) from Chiang Mai, Thailand

Takaoka H, Srisuka W, Saeung A, Otsuka Y, Choochote W

Zootaxa, 2013;3694:280-8.
PMID: 26312290

Simulium (Nevermannia) khunklangense sp. nov. is described from females, males, pupae and larvae collected in Doi Inthanon National Park, Chiang Mai, Thailand. This new species is placed in the vernum species-group of the subgenus Nevermannia and is similar to S. (N.) chomthongense Takaoka & Srisuka described from Doi Inthanon National Park, Thailand, but is distinguished in the male by the number of enlarged upper-eye facets and the relative width of the hind basitarsus against the hind tibia and femur, and in the pupa by the short common basal stalk of the gill and the cocoon with an anterodorsal bulge or a short anterodorsal projection. Taxonomic notes are provided to separate this new species from five other known species of the vernum species-group, which share an accessory sclerite on the larval abdomen, a rare characteristics in this species-group.

Matched MeSH terms: Pupa/growth & development
Conochironomus (Diptera: Chironomidae) in Asia: new and redescribed species and vouchering issues

Cranston PS

Zootaxa, 2016 May 09;4109(3):315-31.
PMID: 27394867 DOI: 10.11646/zootaxa.4109.3.3

The presence of the Afro-Australian genus Conochironomus Freeman, 1961 (Diptera: Chironomidae) in Asia has been recognised only informally. An unpublished thesis included Conochironomus from Singapore, and the genus has been keyed from Malaysia without named species. Here, the Sumatran Conochironomus tobaterdecimus (Kikuchi & Sasa, 1980) comb. n. is recorded from Singapore and Thailand. The species is transferred from Sumatendipes Kikuchi & Sasa, 1980, rendering the latter a junior synonym (syn. n.) of Conochironomus Freeman. Conochironomus nuengthai sp. n. and Conochironomus sawngthai sp. n. are described as new to science, based on adult males from Chiang Mai, Thailand. All species conform to existing generic diagnoses for all life stages, with features from male and female genitalia, pupal cephalic tubercles and posterolateral 'spurs' of tergite VIII providing evidence for species distinction. Some larvae are linked to C. tobaterdecimus through molecular barcoding. Variation in other larvae, which clearly belong to Conochironomus and are common throughout Thailand, means that they cannot be segregated to species. Larval habitats include pools in river beds, urban storage reservoirs, drains with moderately high nutrient loadings, and peat swamps. Endochironomus effusus Dutta, 1994 from north-eastern India may be a congener but may differ in adult morphology, thereby precluding formal new combination until discrepancies can be reconciled. Many problems with vouchering taxonomic and molecular material are identified that need to be rectified in the future.

Matched MeSH terms: Pupa/growth & development
Larval growth of Liosarcophaga dux Thompson (Diptera: Sarcophagidae) under uncontrolled indoor temperatures in Malaysia

Kumara TK, Hassan AA, Salmah MR, Bhupinder S

Southeast Asian J. Trop. Med. Public Health, 2013 Mar;44(2):182-7.
PMID: 23691627

The larval growth of Liosarcophaga dux Thompson (Diptera: Sarcophagidae) was studied under varying indoor room temperatures in Malaysia. Five replicates were established. The immature growth of this species from first instar until adult emergence was 307.0+/-3.0 hours. The mean larval length measured for second instar, third instar, post-feeding stage and puparia were 6.5+/-0.5 mm (n=10), 11.8+/-3.7 mm (n=31), 12.7+/-0.8 mm (n=16), and 9.5+/-0.5 mm (n=15), respectively.

Matched MeSH terms: Pupa/growth & development
The inheritance of autogeny in Aedes (Finlaya) togoi (Theobald) from Malaysia and some aspects of its biology

Thomas V, Leng YP

Southeast Asian J. Trop. Med. Public Health, 1972 Jun;3(2):163-74.
PMID: 5082846
Matched MeSH terms: Pupa/growth & development
Developmental rate, size, and sexual dimorphism of Megaselia scalaris (Loew) (Diptera: Phoridae): its possible implications in forensic entomology

Zuha RM, Omar B

Parasitol Res, 2014 Jun;113(6):2285-94.
PMID: 24728523 DOI: 10.1007/s00436-014-3883-z

Cosmopolitan scuttle fly, Megaselia scalaris (Loew) (Diptera: Phoridae) is one of the commonest forensic species recorded colonizing human corpse indoors and in concealed environment. The occurrence of this species in such environments provides a higher evidential value to assist estimation of postmortem interval (PMI) compared to other forensically important dipterans. However, developmental and size data of M. scalaris are still lacking and they are derived from a limited range of thermal values. The objective of this study is to develop the growth model of M. scalaris by emphasizing the size range of larvae and puparia at different constant temperatures. This species was reared in six replicates at eight varying constant temperatures ranging from 23 to 36 °C and cow's liver was provided as food source. Larvae and puparia were sampled at set time intervals and measured by their length and weight. Because interpretation of forensic entomological evidence is subject to application of different techniques, development of M. scalaris is expressed herein by using developmental table, length/morphological stage diagrams and linear/nonlinear estimation methods. From the findings, it is very important to highlight that sexual dimorphism of M. scalaris during post feeding larva and pupa stage could be observed based on size and developmental periods. Mean length and weight ratios of male to female puparia are approximately 0.8 and 0.3-0.5, respectively, indicating sexual dimorphism of this species. Developmental period in female are 4.0-11.4 h (post feeding larval stage), 3.7-24.0 h (pupal stage), and 3.0-20.1 h (total developmental period) longer in male. Due to this dimorphism, PMI estimation using M. scalaris post feeding larva or puparium specimens must be carried out carefully by to avoid inaccuracy and misinterpretation.

Matched MeSH terms: Pupa/growth & development
Sexually anomalous individuals of the black fly Simulium trangense (Diptera: Simuliidae) infected with mermithid parasites (Nematoda: Mermithidae)

Ya'cob Z, Low VL, Tan TK, Noor-Izwan A, Lourdes EY, Ramli R, et al.

Parasitol Res, 2021 May;120(5):1555-1561.
PMID: 33655351 DOI: 10.1007/s00436-021-07087-x

Sexually anomalous individuals, typically intersexes or gynandromorphs, bear a mixture of male and female traits. Twelve sexually anomalous individuals of the black fly Simulium (Gomphostilbia) trangense Jitklang, Kuvangkadilok, Baimai, Takaoka & Adler were discovered among 49 adults reared from pupae. All 12 sexually anomalous adults were parasitized by mermithid nematodes, although five additional parasitized adults had no overt external anomalies. Sequence analysis of the 18S rRNA gene revealed that the mermithids, possibly representing a new species, are related to Mesomermis spp., with genetic distances of 5.09-6.87%. All 12 anomalous individuals had female phenotypical traits on the head, thorax, forelegs, midlegs, and claws, but male features on the left and right hind basitarsi. One individual had mixed male and female genitalia. The findings are in accord with the trend that mermithid infections are associated with sexually anomalous adult black flies.

Matched MeSH terms: Pupa/growth & development
Fulltext Distribution pattern of black fly (Diptera: Simuliidae) assemblages along an altitudinal gradient in Peninsular Malaysia

Ya'cob Z, Takaoka H, Pramual P, Low VL, Sofian-Azirun M

Parasit Vectors, 2016 Apr 19;9:219.
PMID: 27094088 DOI: 10.1186/s13071-016-1492-7

BACKGROUND: Preimaginal black flies (Diptera: Simuliidae) are important components of the stream ecosystem. However, there has been limited research undertaken on the vertical distribution of preimaginal black flies and their associated ecological factors. Stream conditions are generally variable along the altitudinal gradient. Therefore, we conducted an in-depth entomological survey to investigate the simuliid distribution pattern along an altitudinal gradient in Peninsular Malaysia.
METHODS: A total of 432 collections were performed in this study (24 samplings at each of 18 fixed-streams at monthly intervals) from February 2012 to January 2014. Larvae and pupae attached on aquatic substrates such as grasses, leaves and stems, twigs, plant roots and rocks were collected by hand using fine forceps. Stream depth (m), width (m), velocity (m/s), water temperature (°C), acidity (pH), conductivity (mS/cm) and dissolved oxygen (mg/L) were measured at the time of each collection.
RESULTS: A total of 35 black fly species were recorded in the present study. The most frequently collected species were Simulium tani (31.7%) and S. whartoni (21.5%), while the relatively common species were Simulium sp. (nr. feuerborni) (16.2%), S. decuplum (15.5%), S. angulistylum (14.8%), S. bishopi (13.2%) and S. izuae (11.8%). Total estimated species richness ranged between 39.8 and 41.3, which yielded more than 80% of sampling efficiency. Six simuliid species were distributed below 500 m, whereas eight species were distributed above 1400 m. Simulium sp. (nr. feuerborni) and S. asakoae were found from middle to high altitudes (711-1813 m). Simulium whartoni, S. brevipar and S. bishopi were distributed widely from low to high altitudes (159-1813 m). Regression analysis between species richness and PCs revealed that the species richness was significantly associated with wider, deeper and faster streams at low altitude, normal water temperature (23-25 °C), low conductivity, higher discharge, more canopy cover and riparian vegetation and with larger streambed particles (F = 20.8, df = 1, 422, P

Matched MeSH terms: Pupa/growth & development
Fulltext Bacterial Larvicide, Bacillus thuringiensis israelensis Strain AM 65-52 Water Dispersible Granule Formulation Impacts Both Dengue Vector, Aedes aegypti (L.) Population Density and Disease Transmission in Cambodia

Setha T, Chantha N, Benjamin S, Socheat D

PLoS Negl Trop Dis, 2016 09;10(9):e0004973.
PMID: 27627758 DOI: 10.1371/journal.pntd.0004973

A multi-phased study was conducted in Cambodia from 2005-2011 to measure the impact of larviciding with the bacterial larvicide, Bacillus thuringiensis israelensis (Bti), a water dispersible granule (WG) formulation on the vector, Aedes aegypti (L.) and the epidemiology. In our studies, all in-use containers were treated at 8 g/1000 L, including smaller containers and animal feeders which were found to contribute 23% of Ae aegypti pupae. The treated waters were subjected to routine water exchange activities. Pupal production was suppressed by an average 91% for 8 weeks. Pupal numbers continued to remain significantly lower than the untreated commune (UTC) for 13 weeks post treatment in the peak dengue vector season (p<0.05). Suppression of pupal production was supported by very low adult numbers in the treated commune. An average 70% of the household harbored 0-5 Ae aegypti mosquitoes per home for 8 weeks post treatment, but in the same period of time >50% of the household in the UTC harbored ≥11 mosquitoes per home. The adult population continued to remain at significantly much lower numbers in the Bti treated commune than in the UTC for 10-12 weeks post treatment (p<0.05). In 2011, a pilot operational program was evaluated in Kandal Province, a temephos resistant site. It was concluded that 2 cycles of Bti treatment in the 6 months monsoon season with complete coverage of the target districts achieved an overall dengue case reduction of 48% in the 6 treated districts compared to the previous year, 2010. Five untreated districts in the same province had an overwhelming increase of 352% of dengue cases during the same period of time. The larvicide efficacy, treatment of all in-use containers at the start of the monsoon season, together with treatment coverage of entire districts interrupted disease transmission in the temephos resistant province.

Matched MeSH terms: Pupa/growth & development
Morphology of immature stages of Megaselia spiracularis Schmitz (Diptera: Phoridae)

Dian-Xing F, Guang-Chun L

Microsc Res Tech, 2012 Sep;75(9):1297-303.
PMID: 22522698 DOI: 10.1002/jemt.22064

In addition to causing myiasis in humans, Megaselia spiracularis Schmitz has also been reported as a forensically important fly. In this study, we presented the morphology of all larval instars and puparium of M. spiracularis using scanning electron microscopy. The first instar larva was composed of 12 segments. Antennae and maxillary palp complex were visible. Two spiracular slits could be seen at the posterior spiracle. The branch of posterior spiracular hairs was approximately equal to the palm-formed base in length. Second and third larval instars were very similar to first instar, except for the presence of anterior spiracle. The labium of the second instar larva was triangular and ventrally curved, whereas it was a bilobed structure and the tip forked in the first instar. The bubble membrance comprised of ≈40 globules presented at the third instar larvae. Puparia showed a retracted cephalic region and a pair of pupal respiratory horns on the dorsum. A comparison of the morphological features between immature stages of M. spiracularis and M. scalaris, a forensically important fly indoors in Germany, Malaysia, and China, was discussed.

Matched MeSH terms: Pupa/growth & development
A new species of Simulium (Gomphostilbia) (Diptera: Simuliidae) from Malaysia, with keys to 32 species of the Simulium ceylonicum species-group

Takaoka H, Sofian-Azirun M, Ya'cob Z, Hashim R, Otsuka Y

J Med Entomol, 2014 May;51(3):517-28.
PMID: 24897845

Simulium (Gomphostilbia) leparense sp. nov. is described from females, males, and pupae collected from Peninsular Malaysia. This new species is assigned to the ceylonicum species-group of the subgenus Gomphostilbia, and is characterized by the female and male scuta covered with dark-brown short hairs, smaller number of male upper-eye facets, presence of shiny paired spots on the male abdominal segments 2-8, and absence of grapnel-shaped hooklets on the pupal abdominal segment 9. The male and pupa of S. capillatum Takaoka, which was originally described from larvae collected from Sarawak and Sabah, are described for the first time. Keys to identify all 32 species of the Simulium ceylonicum species-group including 27 species from other countries are provided for females, males, pupae, and mature larvae.

Matched MeSH terms: Pupa/growth & development
Effects of compaction and wetting of laterite cover soil on development and survival of Musca domestica (Diptera: Muscidae) immatures

Abu Tahir N, Ahmad AH

J Med Entomol, 2013 Sep;50(5):999-1002.
PMID: 24180104

Effects of laterite cover soil with different characteristics on survival of buried eggs, third instar larvae, and pupae of Musca domestica (L.) were studied experimentally. Soil treatments were loose dry soil, loose wet soil, compacted dry soil, and compacted wet soil (CWS). Eggs, third instar larvae, and pupae were buried under 30 cm of the different soil treatments and placed under field conditions until adults emerged. Rearing medium was provided for eggs and larvae, and control treatments of all stages were unburied immatures placed on soil surface. Egg and pupal survival to adult were significantly affected by the cover soil treatments, but third instars were more resilient. Wet soil treatments (loose wet soil and CWS) resulted in significantly reduced pupal survival, but increased survival of eggs. However, CWS significantly reduced adult emergence from buried eggs. Though emergence of house flies buried as eggs was significantly reduced, some were able to hatch and emerging first instar larvae developed to pupation. Although cover soil does not completely prevent fly emergence, it did limit development and emergence of buried house flies.

Matched MeSH terms: Pupa/growth & development
Lethal and sublethal impacts of predaceous backswimmer Anisops wakefieldi (Hemiptera: Notonectidae) on the life-history traits of the New Zealand mosquito Culex pervigilans (Diptera: Culicidae)

Zuharah WF, Fadzly N, Lester PJ

J Med Entomol, 2013 Sep;50(5):1014-24.
PMID: 24180106

The presence of predators can have dramatic consequences on prey communities, not only by the direct effects of consumption but also through sublethal effects. We investigated the survival rate and subsequent life history of the mosquito Culex pervigilans Bergroth under the influence of its major predator, the backswimmer Anisops wakefieldi White. We established a field experiment with various treatments: 1) control without predators, 2) free-roaming A. wakefieldi (with one, three, or nine A. wakefieldi per container), 3) caged A. wakefieldi (empty cage without predators, with one, three, or nine A. wakefieldi in each cage, and 4) A. wakefieldi cues (with cues concentrations of one, three, or nine A. wakefieldi). Cx. pervigilans eggs were then taken from these four experimental treatments and reared in two different laboratory conditions: 1) in clean water without any traces of predators, or 2) in water with the same treatments as in field. The survival rate of Cx. pervigilans was significantly reduced by the presence of predators or their cues. Even after a brief exposure to waters containing predators or residual cues, the subsequent progeny and the ontogeny of the remaining survivors were still affected. The percentage of eggs that hatched, and the resulting mosquito population, was influenced by the presence of predators or their cues. Our results suggest that sublethal effects may be carried by surviving individuals primarily through the effects of stress, perhaps by epigenetic mechanisms. We may expect to observe similar plasticity in species or populations with high temporal or spatial variability in predation.

Matched MeSH terms: Pupa/growth & development
Influence of temperature on survival and water relations of Paederus fuscipes (Coleoptera: Staphylinidae)

Bong LJ, Neoh KB, Jaal Z, Lee CY

J Med Entomol, 2013 Sep;50(5):1003-13.
PMID: 24180105

The effects of four temperatures (15, 23.5, 28, and 35 degrees C) on the biological characteristics of the rove beetle Paederus fuscipes Curtis were studied, and its cuticular permeability also was measured. Specimens successfully developed to adulthood at each temperature tested, but development time of each preadult stage significantly decreased with increasing temperature. Both egg and L1 stages required at least 80 degree days above a threshold of approximately 10 degrees C to develop to the subsequent stage. The lengthy development time and high survival rate of preadults at 15 degrees C suggests that P. fuscipes can survive in a harsh environment during cold weather by hibernating, and this ability could allow preadults to succeed ecologically in temperate countries. However, adult longevity was short, and no fecundity was recorded at 15 degrees C. At 28 degrees C, P. fuscipes exhibited a high survival rate of adults, which had a longer life span and high fecundity; thus, the population had the highest intrinsic rate of increase (0.0788 +/- 0.0051 d(-1)) and the shortest mean generation time (48.57 +/- 1.43 d) at 28 degrees C. At this temperature, the population might reach a size that could facilitate invasion into residential areas. However, in the absence of a hygric environment, P. fuscipes was unable to survive despite favorable temperature. Unlike in adults and pupae, high cuticular permeability values were found in the larval stages. This indicates that larvae are highly susceptible to desiccation, and it explains why the distribution of P. fuscipes is restricted to moist habitats.

Matched MeSH terms: Pupa/growth & development
Two new Species of Simulium (Simulium) (Diptera: Simuliidae) From Mount Murud, Sarawak, Malaysia

Takaoka H, Ya'cob Z, Sofian-Azirun M

J Med Entomol, 2015 Jan;52(1):38-49.
PMID: 26336278 DOI: 10.1093/jme/tju009

Two new species of black flies, Simulium (Simulium) murudense and Simulium (Simulium) cheedhangi, are described on the basis of females, males, pupae, and larvae collected in Mount Murud, Sarawak, Malaysia. Both species belong to the Simulium melanopus Edwards species group. S. (S.) murudense sp. nov. is distinguished from most known species by a combination of the haired basal portion of the radial vein and the darkened fore coxae, and S. (S.) cheedhangi sp. nov. is characterized in the female by having a medium-sized claw tooth and in the pupa by six somewhat inflated gill filaments. Notes are given on the S. melanopus species-group in Sarawak and Sabah.

Matched MeSH terms: Pupa/growth & development
The Importance of Habitat in the Ecology of Decomposition on Rabbit Carcasses in Malaysia: Implications in Forensic Entomology

Silahuddin SA, Latif B, Kurahashi H, Walter DE, Heo CC

J Med Entomol, 2015 Jan;52(1):9-23.
PMID: 26336275 DOI: 10.1093/jme/tju001

The stages of decomposition and the faunal succession on rabbit carcasses in three different habitats, namely jungle, rural, and highland areas, were studied. Three New Zealand White rabbit (Oryctolagus cuniculus) carcasses weighing ∼2 kg were sampled daily until the decomposition process was completed. Representative specimens of adult flies, larvae, pupa, and mites were collected from the carcasses and processed in the laboratory. There were differences in decomposition rate and faunal succession between the carcasses. The fastest rate of decomposition was recorded in rural area, and the slowest rate of decomposition was recorded in highland area. The carcasses exhibited the same pattern of colonization by adult flies, but the dominant species of larvae and adult flies on each carcass in specific habitats were different. The primary species of flies recorded in jungle were Chrysomya megacephala F., Chrysomya rufifacies (Macquart), Chrysomya chani Kurahashi, Chrysomya villenuevi Patton, Chrysomya nigripes Aubertin, Chrysomya pinguis (Walker), Hemipyrellia ligurriens (Wiedemann), Hemipyrellia tagaliana (Bigot), Hypopyiopsis fumipennis (Walker), Hypopygiopsis violacea (Macquart), and Hydrotaea spinigera Stein represented by both adults and larvae. Musca domestica L., Atherigona sp., Lioproctia pattoni (Senior-White), Lioproctia saprianovae Pape & Bänziger, and Seniorwhitea princeps (Wiedemann) were represented by adults only. The biodiversity of flies in the rural area were C. megacephala, C. rufifacies, H. ligurriens, Fannia canicularis L., Hydrotaea chalcogaster (Wiedemann), and Hyd. spinigera represented by both adults and larvae, meanwhile M. domestica, Atherigona sp., Boettcherisca peregrina (Robineau-Desvoidy), Parasarcophaga taenionota Wiedemann, Parasarcophaga scopariiformis Senior-White, and S. princeps were represented by adults only. The species of flies collected in the highland area were Lucilia porphyrina (Walker), C. megacephala, C. rufifacies, C. villenuevi, C. pinguis, H. ligurriens, Hyd. spinigera, Hyd. chalcogaster, F. canicularis, and Boettcherisca highlandica Kurahashi & Tan represented by both adults and larvae, whereas C. nigripes, Chrysomya thanomthini Kurahashi & Tumrasvin, M. domestica, Atherigona sp., Parasarcophaga albiceps Meigen, P. taenionota, Sepsidae, Phoridae, and Millichidae were represented by adults only. Faunal succession followed the sequence of dominant flies, i.e., Calliphoridae, Sarcophagidae, Muscidae, Sepsidae, and lastly Stratiomyidae for jungle, or Sepsidae for rural and highland studies. Mites, from suborders Mesostigmata, Prostigmata, Astigmatina, and Oribatida, were also recovered throughout decomposition, which could be used for future implementation in forensic investigations. The data obtained from this study could provide more accurate indicators for local forensic scientists in solving criminal cases especially on the determination of time and primary location of death.

Matched MeSH terms: Pupa/growth & development
A New Species of Simulium (Gomphostilbia) (Diptera: Simuliidae) From Kalimantan, Indonesia, With Keys to Identify 19 Bornean Species of the Subgenus Gomphostilbia

Takaoka H, Sofian-Azirun M, Ya'cob Z, Chen CD, Low VL, Harmonis

J Med Entomol, 2016 07;53(4):798-806.
PMID: 27099400

A new simuliid species, Simulium kalimantanense sp. nov., is described on the basis of females, males, pupae, and mature larvae from East Kalimantan, Indonesia, and is assigned to the Simulium banauense species-group of Simulium (Gomphostilbia). This new species has close similarities to S alienigenum Takaoka from the Philippines, in many characters including the adult antennal color pattern and pupal gill with four long filaments arranged in two pairs each bearing a long stalk, but is distinguished from the latter in the female by the longer sensory vesicle and in the pupa by the gill with an elongate common basal stalk. Simulium kalimantanense sp. nov. is the first member of the S. banauense group in Borneo, and marks the most southerly distribution of the group. Keys to identify 19 Bornean species of the subgenus Gomphostilbia are provided.

Matched MeSH terms: Pupa/growth & development
Three Taxa in One: Cryptic Diversity in the Black Fly Simulium nobile (Diptera: Simuliidae) in Southeast Asia

Low VL, Takaoka H, Pramual P, Adler PH, Ya'cob Z, Chen CD, et al.

J Med Entomol, 2016 07;53(4):972-976.
PMID: 27208009

We access the molecular diversity of the black fly Simulium nobile De Mejiere, using the universal cytochrome c oxidase subunit I (COI) barcoding gene, across its distributional range in Southeast Asia. Our phylogenetic analyses recovered three well-supported mitochondrial lineages of S. nobile, suggesting the presence of cryptic species. Lineage A is composed of a population from Sabah, East Malaysia (Borneo); lineage B represents the type population from Java, Indonesia; and lineage C includes populations from the mainland of Southeast Asia (Peninsular Malaysia and Thailand). The genetic variation of lineage C on the mainland is greater than that of lineages A and B on the islands of Borneo and Java. Our study highlights the value of a molecular approach in assessing species status of simuliids in geographically distinct regions.

Matched MeSH terms: Pupa/growth & development
Revision of Simulium rufibasis (Diptera: Simuliidae) in Japan and Korea: Chromosomes, DNA, and Morphology

Adler PH, Fukuda M, Takaoka H, Reeves WK, Kim SK, Otsuka Y

J Med Entomol, 2020 02 27;57(2):388-403.
PMID: 31746337 DOI: 10.1093/jme/tjz197

The widespread nominal black fly Simulium (Simulium) rufibasis Brunetti was reexamined morphologically, chromosomally, and molecularly to determine the status of populations in Japan and Korea with respect to S. rufibasis from the type locality in India and to all other known species in the S. (S.) tuberosum species-group. Morphological comparisons established that the species previously known as S. rufibasis in Japan and Korea is distinct from all other species. Consequently, it was described and illustrated as a new species, Simulium (S.) yamatoense. Simulium yokotense Shiraki, formerly a synonym of S. rufibasis, was morphologically reevaluated and considered a species unplaced to species-group in the subgenus Simulium. Chromosomal analyses of S. yamatoense sp. nov. demonstrated that it is unique among all cytologically known species of the S. tuberosum group and is the sister species of the Taiwanese species tentatively known as S. (S.) arisanum Shiraki. Populations of S. yamatoense sp. nov. included two cytoforms, based on the sex chromosomes. Cytoform A, including topotypical representatives, was found in Kyushu, Japan, whereas cytoform B was found in Korea and Honshu, Japan. Molecular analysis based on the COI mitochondrial gene generally corroborated morphological and chromosomal data that S. yamatoense sp. nov. is a distinct species and, like the chromosomal data, indicate that it is most closely related to S. arisanum, with interspecific genetic distance of 2.92-4.63%.

Matched MeSH terms: Pupa/growth & development
First Life Table of Synthesiomyia nudiseta (Diptera: Muscidae) From Palearctic Region for Analyzing the Effect on Its Dispersal Ability

Ivorra T, García-Martínez B, Martínez-Sánchez A

J Med Entomol, 2021 11 09;58(6):2247-2254.
PMID: 34279664 DOI: 10.1093/jme/tjab129

To better understand the population dynamics and dispersal ability of insect species, it is often helpful to derive a life table containing fundamental demographic data. The aim of this study was to determine a life table for the predatory necrophagous species Synthesiomyia nudiseta (van der Wulp, 1883) on a pig liver diet and under controlled laboratory conditions (29.5 ± 2. 5°C, RH 50 ± 15%, and a photoperiod of 12:12). This species has medical and veterinary importance and its distribution extends in tropical and subtropical areas and now it has been established in the southwestern of Europe. The mean adult longevity was 36. 18 ± 2. 06 d and the net reproduction rate, R, was 27.65 offspring/female, the mean generation time, T, was 22. 09 d, the finite rate of increase, λ, was 1. 16 d-1, and the intrinsic rate of increase, r, was 0. 15 d-1. These results indicate that S. nudiseta cannot be considered an r-strategist as the most common synanthropic necrophagous blowflies due to its predatory behavior; however, its invasive and colonist abilities are discussed. This is the first life table study of this species from Palearctic region to analyze the effect of its dispersal ability.

Matched MeSH terms: Pupa/growth & development

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