Displaying publications 1 - 20 of 184 in total

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  1. Hooper C, Debnath PP, Biswas S, van Aerle R, Bateman KS, Basak SK, et al.
    Viruses, 2020 10 02;12(10).
    PMID: 33023199 DOI: 10.3390/v12101120
    Mass mortalities of the larval stage of the giant freshwater prawn, Macrobrachium rosenbergii, have been occurring in Bangladesh since 2011. Mortalities can reach 100% and have resulted in an 80% decline in the number of hatcheries actively producing M. rosenbergii. To investigate a causative agent for the mortalities, a disease challenge was carried out using infected material from a hatchery experiencing mortalities. Moribund larvae from the challenge were prepared for metatranscriptomic sequencing. De novo virus assembly revealed a 29 kb single‑stranded positive-sense RNA virus with similarities in key protein motif sequences to yellow head virus (YHV), an RNA virus that causes mass mortalities in marine shrimp aquaculture, and other viruses in the Nidovirales order. Primers were designed against the novel virus and used to screen cDNA from larvae sampled from hatcheries in the South of Bangladesh from two consecutive years. Larvae from all hatcheries screened from both years were positive by PCR for the novel virus, including larvae from a hatchery that at the point of sampling appeared healthy, but later experienced mortalities. These screens suggest that the virus is widespread in M. rosenbergii hatchery culture in southern Bangladesh, and that early detection of the virus can be achieved by PCR. The hypothesised protein motifs of Macrobrachium rosenbergii golda virus (MrGV) suggest that it is likely to be a new species within the Nidovirales order. Biosecurity measures should be taken in order to mitigate global spread through the movement of post-larvae within and between countries, which has previously been linked to other virus outbreaks in crustacean aquaculture.
    Matched MeSH terms: Open Reading Frames
  2. Noor YM, Samsulrizal NH, Jema'on NA, Low KO, Ramli AN, Alias NI, et al.
    Gene, 2014 Jul 25;545(2):253-61.
    PMID: 24811681 DOI: 10.1016/j.gene.2014.05.012
    Bacillus lehensis G1 is a Gram-positive, moderately alkalitolerant bacterium isolated from soil samples. B. lehensis produces cyclodextrin glucanotransferase (CGTase), an enzyme that has enabled the extensive use of cyclodextrin in foodstuffs, chemicals, and pharmaceuticals. The genome sequence of B. lehensis G1 consists of a single circular 3.99 Mb chromosome containing 4017 protein-coding sequences (CDSs), of which 2818 (70.15%) have assigned biological roles, 936 (23.30%) have conserved domains with unknown functions, and 263 (6.55%) have no match with any protein database. Bacillus clausii KSM-K16 was established as the closest relative to B. lehensis G1 based on gene content similarity and 16S rRNA phylogenetic analysis. A total of 2820 proteins from B. lehensis G1 were found to have orthologues in B. clausii, including sodium-proton antiporters, transport proteins, and proteins involved in ATP synthesis. A comparative analysis of these proteins and those in B. clausii and other alkaliphilic Bacillus species was carried out to investigate their contributions towards the alkalitolerance of the microorganism. The similarities and differences in alkalitolerance-related genes among alkalitolerant/alkaliphilic Bacillus species highlight the complex mechanism of pH homeostasis. The B. lehensis G1 genome was also mined for proteins and enzymes with potential viability for industrial and commercial purposes.
    Matched MeSH terms: Open Reading Frames
  3. Hamidah Othman, Noor Azizah Mohd Ali
    MyJurnal
    HIV/AIDS is a matter of concern and has become a pandemic disease that threatens the world population. It
    has remained one of the priorities in global public health challenges. Caring for people with HIV/AIDS
    requires clinical expertise as well as high-quality skills for health care providers. The purpose of the study was
    to examine knowledge and attitude on HIV/AIDS patients among nursing students. A cross-sectional study
    was conducted in Public University at Wast Cost Malaysia. The 126 respondents of nursing students agreed to
    participate in the study using a self-administered questionnaire that was designed and modied after reading
    and referring to articles, books, and journals. The results of the research revealed that nursing students, in
    general, have a satisfactory knowledge (mean score 30.71), but had a moderate attitude toward HIV/AIDS
    patient with total mean score 2.34. Most of the students (89.3%) get the knowledge regarding HIV/AIDS
    through the internet. The majority of the students knew that blood transfusion (99.4%) and sharing
    needles/syringe (99%) were the higher sources HIV/AIDS modes of transmission. Most of the students agreed
    that peoples with HIV/AIDS should be treated in society with support from the community (97.0%). Although
    the knowledge level of nursing students seems to be satisfactory, but they still showed the moderate attitude
    towards HIV/AIDS patients. Thus, structured education programmes should be conducted by nursing faculty
    to ensure that nursing students gain the necessary accurate knowledge and an appropriate attitude towards the
    care of HIV/AIDS patients.
    Matched MeSH terms: Reading
  4. Radaideh, K.M., Matalqah, L.M., Tajuddin, A.A., Lee Luen, F.W., Bauk, S., Abdel Munem, E.M.E
    MyJurnal
    The ultimate check of the actual dose delivered to a patient in radiotherapy can be achieved by using dosimetric measurements. The aims of this study were to develop and evaluate a custom handmade head and neck phantom for evaluation of Three-Dimensional Conformal Radiation Therapy (3D-CRT) dose planning and delivery. A phantom of head and neck region of a medium built male patient with nasopharyngeal cancer was constructed from Perspex material. Primary and secondary Planning Target Volume (PTV) and twelve Organs at Risk (OAR) were delineated using Treatment Planning System (TPS) guided by computed tomography printout transverse images. One hundred and seven (107) holes distributed among the organs were loaded with Rod-shaped Thermoluminescent dosimeters (LiF:Mg,Ti TLDs) after common and individual calibration. Head and neck phantom was imaged, planned and irradiated conformally (3D-CRT) by linear accelerator (LINAC Siemens Artiste). The planned predicted doses by TPS at PTV and OAR regions were obtained and compared with the TLD measured doses using the phantom. Repeated TLD measurements were reproducible with a percent standard deviation of < 3.5%. Moreover, the average of dose discrepancies between TLDs reading and TPS predicted doses were found to be < 5.3%. The phantom’s preliminary results have proved to be a valuable tool for 3D-CRT treatment dose verification.
    Matched MeSH terms: Reading
  5. Matsumoto T, Sato M, Nishizono A, Ahmed K
    Arch Virol, 2019 Aug;164(8):2179-2182.
    PMID: 31111258 DOI: 10.1007/s00705-019-04286-x
    We identified two novel circoviruses, HK02976 and HK00220, in oral swabs from bats. The size of their full genome was 2,010 nucleotides (nt). The full-genome sequence of our strains shared 96.1% nucleotide sequence identity with each other, and 39.9%-69.5% identity with bat-associated circoviruses (BatACVs)1-9. Based on the species demarcation threshold for viruses of the family Circoviridae, which is 80% genome-wide nucleotide sequence identity, we have tentatively named this group of viruses "bat-associated circovirus 10" (BatACV10).
    Matched MeSH terms: Open Reading Frames/genetics
  6. Chai JT, Chen CJ
    MyJurnal
    Dyslexia is a language disorder that leads to difficulty with words and it is the most common type of learning disability. This article presents a systematic review on the current state of assistive technologies used in improving the learning process of learn-ers with dyslexia. A total of 25 journals articles and international conference papers published between 2000 and 2014 were included in the review. The research articles were collected from 12 databases and analyzed based on the qualitative cyclical pro-cess. A majority of the studies focused on children and adolescents. Four main themes on the types of technologies used in aiding the learning process of learners with dys-lexia are derived and discussed. These include text-to-speech, eye-tracking, virtual learning environments, and games. The text-to-speech technology is the most common type of technology used by learners with dyslexia. In terms of the roles played by the assistive technologies, another four emerging themes are identified, which cover the roles of aiding reading, writing, memory, and mathematics. The review also discovers that a majority of these studies focus on the use of technologies for improving the reading ability of learners with dyslexia.
    Matched MeSH terms: Reading
  7. Lee LW, Wheldall K
    Dyslexia, 2011 Feb;17(1):19-37.
    PMID: 21241030 DOI: 10.1002/dys.421
    Malay is a consistent alphabetic orthography with complex syllable structures. The focus of this research was to investigate word recognition performance in order to inform reading interventions for low-progress early readers. Forty-six Grade 1 students were sampled and 11 were identified as low-progress readers. The results indicated that both syllable awareness and phoneme blending were significant predictors of word recognition, suggesting that both syllable and phonemic grain-sizes are important in Malay word recognition. Item analysis revealed a hierarchical pattern of difficulty based on the syllable and the phonic structure of the words. Error analysis identified the sources of errors to be errors due to inefficient syllable segmentation, oversimplification of syllables, insufficient grapheme-phoneme knowledge and inefficient phonemic code assembly. Evidence also suggests that direct instruction in syllable segmentation, phonemic awareness and grapheme-phoneme correspondence is necessary for low-progress readers to acquire word recognition skills. Finally, a logical sequence to teach grapheme-phoneme decoding in Malay is suggested.
    Matched MeSH terms: Reading*
  8. Chen AH, Khalid NM, Buari NH
    Int J Ophthalmol, 2019;12(7):1170-1176.
    PMID: 31341810 DOI: 10.18240/ijo.2019.07.19
    AIM: To investigate the effect of age on reading acuity and reading speed in attaining text information in healthy eyes.

    METHODS: Reading acuity, critical print size, reading speed and maximum reading speed were measured in groups of 40 children (8 to 12 years old), 40 teenagers (13 to 19 years old), 40 young adults (20 to 39 years old), and 40 adults (40 years old and above) using the Buari-Chen Malay Reading Chart [contextual sentences (CS) set and random words (RW) set] in a cross-sectional study design.

    RESULTS: Reading acuity was significantly improved by 0.04 logMAR for both CS set and RW set from children to teenagers, then gradually worsened from young adults to adults (CS set: 0.06 logMAR; RW set: 0.08 logMAR). Critical print size for children showed a significant improvement in teenagers (CS set: 0.14 logMAR; RW set: 0.07 logMAR), then deteriorated from young adults to adults by 0.09 logMAR only for CS set. Reading speed significantly increased from children to teenagers, [CS set: 46.20 words per minute (wpm); RW set: 42.06 wpm], then stabilized from teenagers to young adults, and significantly reduced from young adults to adults (CS set: 28.58 wpm; RW set: 24.44 wpm). Increment and decrement in maximum reading speed measurement were revealed from children to teenagers (CS set: 39.38 wpm; RW set: 43.38 wpm) and from young adults to adults (CS set: 22.26 wpm; RW set: 26.31 wpm) respectively.

    CONCLUSION: The reference of age-related findings in term of acuity and speed of reading should be incorporated in clinical practice to enhance reading assessment among healthy eyes population.

    Matched MeSH terms: Reading
  9. Sayed, Inayatullah Shah
    MyJurnal
    Allah (s.w.t) has created innumerable distinct creatures and mentioned to us about their special qualities through His revelation. The Qur’an is the ultimate source of guidance for its followers for all aspects of life including science. If one is to study nature scientifically there are countless observable facts that are parallel to the teachings of Islam. One of these facts is echolocation found in bats and dolphins. These animals generate ultrasonic signals and detect the echoes reflected back to them to map out their environment and catch prey. Modern health sciences have already adopted this phenomenon in the form of ultrasound imaging for diagnosis of certain diseases. However, there is room for improvement in the overall performance of this technique. This article highlights the technological developments directly inspired by nature i.e., crawfish/crayfish and relates echolocation characteristics of bats and dolphins with basic principles of ultrasound imaging. In-depth studies on the echolocation properties of these creatures can lead to further improvement in the current ultrasound imaging technique. Such as; the construction of a transducer which simultaneously generates multi-frequency ultrasound signals and development of new interpreting software. Moreover, reading verses of the Holy Qur’an heartily and enthusiastically will lead to the development of innovative ideas that can be translated into reality and applied for the betterment of humankind.
    Matched MeSH terms: Reading
  10. Julia Lee, Ai Cheng, Wang, Joanna Joo Ying
    MyJurnal
    Alphabetic knowledge and phonological awareness are essential skills in learning to read. This research examined the level of acquisition on alphabetic knowledge and phonological awareness among 60 preschoolers from private and public preschools in Kuching, Sarawak. The mean age of the children was 5.58. The children were administered letter name and sound knowledge, and letter naming fluency tests to examine their alphabetic knowledge; Comprehensive Test of Phonological Processing and Yopp-Singer Phoneme Segmentation Test to examine their phonological awareness. Higher achievement in alphabetic knowledge and phonological awareness was found among preschoolers from private preschools compared to those from public preschools. This study discusses the implications for practice and ways teachers could explicitly foster alphabetic knowledge and phonological awareness skills in classroom.
    Matched MeSH terms: Reading
  11. Abdul Razak F, Baharuddin BA, Akbar EFM, Norizan AH, Ibrahim NF, Musa MY
    Arch Oral Biol, 2017 Aug;80:180-184.
    PMID: 28448807 DOI: 10.1016/j.archoralbio.2017.04.014
    OBJECTIVE: Compact-structured oral biofilm accumulates acids that upon prolonged exposure to tooth surface, causes demineralisation of enamel. This study aimed to assess the effect of alternative sweeteners Equal Stevia(®), Tropicana Slim(®), Pal Sweet(®) and xylitol on the matrix-forming activity of plaque biofilm at both the early and established stages of formation.

    METHODS: Saliva-coated glass beads (sGB) were used as substratum for the adhesion of a mixed-bacterial suspension of Streptococcus mutans, Streptococcus sanguinis and Streptococcus mitis. Biofilms formed on sGB at 3h and 24h represented the early and established-plaque models. The biofilms were exposed to three doses of the sweeteners (10%), introduced at three intervals to simulate the exposure of dental plaque to sugar during three consecutive food intakes. The treated sGB were (i) examined under the SEM and (ii) collected for turbidity reading. The absorbance indicated the amount of plaque mass produced. Analysis was performed comparative to sucrose as control.

    RESULTS: Higher rate of bacterial adherence was determined during the early compared to established phases of formation. Comparative to the sweeteners, sucrose showed a 40% increase in bacterial adherence and produced 70% more plaque-mass. Bacterial counts and SEM micrographs exhibited absence of matrix in all the sweetener-treated biofilms at the early phase of formation. At the established phase, presence of matrix was detected but at significantly lower degree compared to sucrose (p<0.05).

    CONCLUSION: Alternatives sweeteners promoted the formation of oral biofilm with lighter mass and lower bacterial adherence. Hence, suggesting alternative sweeteners as potential antiplaque agents.

    Matched MeSH terms: Reading
  12. Abu Bakar, B., Abdul Rahman, M.S., Teoh, C.C., Abdullah, M.Z.K., Ismail, R.
    Food Research, 2018;2(2):177-182.
    MyJurnal
    Rice plant population density is a key indicator in determining the crop setting and fertilizer application rate. It is therefore essential that the population density is monitored to ensure that a correct crop management decision is taken. The conventional method of determining plant population is by manually counting the total number of rice plant tillers in a 25 cm x 25 cm square frame. Sampling is done by randomly choosing several different locations within a plot to perform tiller counting. This sampling method is time consuming, labour intensive and costly. An alternative fast estimating method was developed to overcome this issue. The method relies on measuring the outer circumference
    or ambit of the contained rice plants in a 25 cm x 25 cm square frame to determine the number of tillers within that square frame. Data samples of rice variety MR219 were collected from rice plots in the Muda granary area, Sungai Limau Dalam, Kedah. The data were taken at 50 days and 70 days after seeding (DAS). A total of 100 data samples were collected for each sampling day. A good correlation was obtained for the variety of 50 DAS and 70 DAS. The model was then verified by taking 100 samples with the latching strap for 50 DAS and 70 DAS. As a result, this technique can be used as a fast, economical and practical alternative to manual tiller counting. The technique can potentially be used in the development of an electronic sensing system to estimate paddy plant population density.
    Matched MeSH terms: Reading Frames
  13. Ahmad, H.S.
    MyJurnal
    The past decade has seen a marked increase in the popularity of ATS use, particularly methamphetamine, within East Asia,and the Pacific region. In Malaysia, the National Anti Drug Agency has identified 8,870 addicts (from January till August 2008) out of which 1,126 was ATS dependence. During the same period, the police have arrested 46,388 people under the Dangerous Drug Act 1952. They also has seize 283kg of syabu, 545kg of ecstacy powder, 66194 tablets of esctacy pills and 222,376 tablets of yaba pills from Jan till August this year. The occurrence of psychosis arising from the use of ATS was first reported in the late 1930's. With growing ATS use, particularly methamphetamine, ATS-induced psychosis has become a major impact on public health.Symptoms of ATS-induced psychosis: Methamphetamine use produces a variety of effects, ranging from irritability, to physical aggression, hyperawareness, hypervigilance, and psychomotor agitation. Repeated or high-dose use of the stimulant can cause drug-induced psychosis resembling paranoid schizophrenia, characterized by hallucinations, delusions and thought disorders. When used in long term, methamphetamine may lead to development of psychiatric symptoms due to dopamine depletion in the striatum. The most common lifetime psychotic symptoms among methamphetamine psychotic patients - as reported in a cross-country study involving Australia, Japan, the Philippines and Thailand - are persecutory delusion, auditory hallucinations, strange or unusual beliefs and thought reading. Those patients were also reported to suffer from impaired speech, psychomotor retardation, depression and anxiety. An ATS psychosis can be distinguished from primary psychotic disorders by time. In ATS-induced psychosis symptoms usually resolve after the drug is discontinued. If symptoms do not resolve within 2 weeks after cessation of stimulant use, a primary psychiatric disorder should be suspected. When compared with other stimulants, such as cocaine, psychosis is induced more commonly by ATS, possibly due to the longer duration of action produced by amphetamines.For example, while smoking cocaine produces a high that lasts for 20-30 minutes, smoking methamphetamine produces a high that lasts 8-24 hours. Other symptoms of ATS-induced psychosis reported include affective blunting,(6) violent behavior, and self-mutilation and self-injurious behavior.
    Matched MeSH terms: Reading
  14. Lee JAC, Lee S, Yusoff NFM, Ong PH, Nordin ZS, Winskel H
    Front Psychol, 2020;11:1700.
    PMID: 32754104 DOI: 10.3389/fpsyg.2020.01700
    The aim of the study was to develop a new comprehensive reading assessment battery for multi-ethnic and multilingual learners in Malaysia. Using this assessment battery, we examined the reliability, validity, and dimensionality of the factors associated with reading difficulties/disabilities in the Malay language, a highly transparent alphabetic orthography. In order to further evaluate the reading assessment battery, we compared results from the assessment battery with those obtained from the Malaysian national screening instrument. In the study, 866 Grade 1 children from multi-ethnic and multilingual backgrounds from 11 government primary schools participated. The reading assessment battery comprised 13 assessments, namely, reading comprehension, spelling, listening comprehension, letter name knowledge, letter name fluency, rapid automatized naming, word reading accuracy, word reading efficiency, oral reading fluency, expressive vocabulary, receptive vocabulary, elision, and phonological memory. High reliability and validity were found for the assessments. An exploratory factor analysis yielded three main constructs: phonological-decoding, sublexical-fluency, and vocabulary-memory. Phonological-decoding was found to be the most reliable construct that distinguished between at-risk and non-at-risk children. Identifying these underlying factors will be useful for detecting children at-risk for developing reading difficulties in the Malay language. In addition, these results highlight the importance of including a range of reading and reading-related measures for the early diagnosis of reading difficulties in this highly transparent orthography.
    Matched MeSH terms: Reading
  15. Foo JN, Tan LC, Liany H, Koh TH, Irwan ID, Ng YY, et al.
    Hum Mol Genet, 2014 Jul 15;23(14):3891-7.
    PMID: 24565865 DOI: 10.1093/hmg/ddu086
    To evaluate the contribution of non-synonymous-coding variants of known familial and genome-wide association studies (GWAS)-linked genes for Parkinson's disease (PD) to PD risk in the East Asian population, we sequenced all the coding exons of 39 PD-related disease genes and evaluated the accumulation of rare non-synonymous-coding variants in 375 early-onset PD cases and 399 controls. We also genotyped 782 non-synonymous-coding variants of these genes in 710 late-onset PD cases and 9046 population controls. Significant enrichment of LRRK2 variants was observed in both early- and late-onset PD (odds ratio = 1.58; 95% confidence interval = 1.29-1.93; P = 8.05 × 10(-6)). Moderate enrichment was also observed in FGF20, MCCC1, GBA and ITGA8. Half of the rare variants anticipated to cause loss of function of these genes were present in healthy controls. Overall, non-synonymous-coding variants of known familial and GWAS-linked genes appear to make a limited contribution to PD risk, suggesting that clinical sequencing of these genes will provide limited information for risk prediction and molecular diagnosis.
    Matched MeSH terms: Open Reading Frames
  16. Noor'Ain Azizan, Seri Rahayu Kamat, Nur Syafiqah Rayme, Ruzy Haryati Hambali
    MyJurnal
    Repetitive movement can lead to the pain muscle, nerves, and tendons that cause by
    repetitive overuse of working task. The muscle will fatigue due to; repetitive
    movement, force that been applied, posture during working and duration of working.
    The stress level during working can influent the energy performance usage during
    working. The aim of this paper is to analyse the influence of heart rate and muscle
    activity of workers in composite manufacturing towards muscle fatigue. The data was
    collected for a worker in hand layup department and the Qualitative method was used
    in a way to investigate the working load and level of pain received by their body. Then,
    the Qualitative data was sorted and the respondent proceeded for a Quantitative
    method which involves muscle activity analysis and heart rate analysis. The tools that
    were used to conduct these experiments were surface electromyography (sEMG),
    Wristwatch with chest strap and perceived stress scale (PSS). The experimentation
    used to calculate the average reading of heart rate and muscle activity during working
    and detect the duration the muscle to start fatigue. Moreover, this paper analysed the
    relationship between heart rate and muscle activity through the duration of working.
    As an overall finding of this research, it was shown that the heart rate of the workers
    influence the muscle activity of workers and has high potential relationship to the
    fatigue of muscles of the workers in the layup department.
    Matched MeSH terms: Reading
  17. Dawood OT, Hassali MA, Saleem F, Ibrahim IR
    Pharmacol Res Perspect, 2018 04;6(2):e00387.
    PMID: 29511566 DOI: 10.1002/prp2.387
    This study was undertaken to assess the people's self-reported reading of medicine labels and its associated factors and to assess the sources of information about medicines among general public. A cross-sectional study was carried out among general public in the State of Penang, Malaysia. A total of 888 participants were conveniently selected and completed the survey. A self-administered questionnaire was used to obtain the data from all the participants. Most of the participants (74.2%) reported that they have adequate information about medicines provided on their medicine labels. In addition, 86.9% of them reported that they read their medicine's label for the directions of usage and 84.3% for the dosage instruction. However, 42.1% of the participants do not read their medicine's label for the active ingredients, and 33% of them do not read their medicine's label for the safety information. In addition, 36.5% of the respondents did not read the label of medicine for the symptoms which can be used for. However, females, Malay respondents, and higher education level (college/university) were more likely to self-reported the reading medicine's label. Females were more likely to read the labels of medicines compared with males (OR = 1.6, 95% CI 1.20-2.13, P = .001). The reading of medicine labels was predicted by females, Malay respondents, and higher educated people. Health educational programs are needed to clarify label's information that can help in concept of patient safety.
    Matched MeSH terms: Reading*
  18. Kaur D, Bishop GD
    Int J Psychophysiol, 2013 Feb;87(2):130-40.
    PMID: 23206971 DOI: 10.1016/j.ijpsycho.2012.11.011
    Epidemiological studies have shown significant ethnic differences in coronary heart disease death rates with South Asians showing significantly greater coronary heart disease mortality than other groups.
    Matched MeSH terms: Reading
  19. Yong HY, Bakar FD, Illias RM, Mahadi NM, Murad AM
    Braz J Microbiol, 2013 Dec;44(4):1241-50.
    PMID: 24688518
    The mitogen-activated protein (MAP) kinase pathways has been implicated in the pathogenicity of various pathogenic fungi and plays important roles in regulating pathogenicity-related morphogenesis. This work describes the isolation and characterization of MAP kinase gene, Cgl-SLT2, from Colletotrichum gloeosporioides. A DNA sequence, including 1,633 bp of Cgl-SLT2 open-reading frame and its promoter and terminator regions, was isolated via DNA walking and cloned. To analyze gene function, a gene disruption cassette containing hygromycin-resistant gene was constructed, and Cgl-SLT2 was inactivated via gene deletion. Analysis on Cgl-slt2 mutant revealed a defect in vegetative growth and sporulation as compared to the wild-type strain. When grown under nutrient-limiting conditions, hyperbranched hyphal morphology was observed in the mutant. Conidia induction for germination on rubber wax-coated hard surfaces revealed no differences in the percentage of conidial germination between the wild-type and Cgl-slt2 mutant. However, the percentage of appressorium formation in the mutant was greatly reduced. Bipolar germination in the mutant was higher than in the wild-type at 8-h post-induction. A pathogenicity assay revealed that the mutant was unable to infect either wounded or unwounded mangoes. These results suggest that the Cgl-SLT2 MAP kinase is required for C. gloeosporioides conidiation, polarized growth, appressorium formation and pathogenicity.
    Matched MeSH terms: Open Reading Frames
  20. Wong CM, Tam HK, Ng WM, Boo SY, González M
    Plasmid, 2013 Mar;69(2):186-93.
    PMID: 23266397 DOI: 10.1016/j.plasmid.2012.12.002
    A cryptic plasmid, pMWHK1 recovered from an Antarctic bacterium Pedobacter cryoconitis BG5 was sequenced and characterised. The plasmid is a circular 6206bp molecule with eight putative open reading frames designated as orf1, orf2, orf3, orf4, orf5, orf6, orf7 and orf8. All the putative open reading frames of pMWHK1 are found to be actively transcribed. Proteins encoded by orf2 and orf4 are predicted to be responsible for the mobilization and replication of the plasmid respectively. orf4 shares 55% and 61% identities with the theta-type Rep proteins from two strains of Riemerella anatipestifer. This suggests that pMWHK1 could be a member of the theta-type replicating plasmid. The origin of replication is located within the AT-rich region upstream of orf4. orf5 and orf6 encode bacterial toxin-antitoxin proteins predicted to maintain plasmid stability. orf3 encodes an entry exclusion protein that is hypothetically involved in reducing the frequency of DNA transfer through conjugation. orf1, orf7 and orf8 encode proteins with unknown functions. Plasmid, pMWHK1 is stably maintained in P. cryoconitis BG5 at 20°C.
    Matched MeSH terms: Open Reading Frames/genetics
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