Displaying publications 1 - 20 of 162 in total

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  1. Geosesarma sodalis, a new species of vampire crab (Crustacea, Brachyura, Sesarmidae) from a limestone cave in central Sarawak, Malaysia
    Ng PKL
    Zookeys, 2021;1031:133-141.
    PMID: 33958910 DOI: 10.3897/zookeys.1031.63134
    A new species of semi-terrestrial crab of the genus Geosesarma (Sesarmidae) is described from a limestone cave in central Sarawak, Malaysian Borneo. Geosesarma sodalissp. nov. is characterised by its quadrate carapace, absence of a flagellum on the exopod of the third maxilliped, presence of 10 or 11 sharp tubercles on the dactylus of the chela and a diagnostic male first gonopod structure. This is the sixth species of Geosesarma reported from Sarawak, and the first member of the genus collected from inside caves.
    Matched MeSH terms: Seafood
  2. Fulltext Serum cortisol level and survival rate of juvenile Epinephelus fuscoguttatus following exposure to different salinities
    Tahir D, Shariff M, Syukri F, Yusoff FM
    Vet World, 2018 Mar;11(3):327-331.
    PMID: 29657425 DOI: 10.14202/vetworld.2018.327-331
    Background and Aim: Brown-marbled grouper Epinephelus fuscoguttatus is a premium marine food fish with high demand in Asia. In fish, stress due to environmental changes such as fluctuations in the salinity can result in increased cortisol level. Stress in fish increases susceptibility to diseases ultimately resulting in death. Therefore, the aim of this study was to investigate the salinity tolerance of E. fuscoguttatus and their survival in lower salinities.

    Materials and Methods: In this study, grouper juveniles (92.43±standard error of the mean 0.51 mm) maintained in 31 ppt seawater were transferred into five tanks with seawater diluted to 25, 20, 15, 10, and 5 ppt. The salinity of the control group was not changed and was maintained at 31 ppt. Serum cortisol was measured using ELISA at 0, 30, 60, and 120 min after the fish were transferred to the different concentrations of salinity.

    Results: The survival percentage was recorded for 14 days following the transfer and the results revealed that serum cortisol of fish in a high change in salinity (15, 10, and 5 ppt) was significantly higher than the control group immediately after exposure. At the high salinity change, the cortisol levels gradually decrease at 30 min and 60 min, until no difference in cortisol concentration was observed at 120 min. No mortality was observed in fish exposed to low salinity change (25 and 20 ppt) while in higher salinity change (5 ppt), the survival percentage was 50%.

    Conclusion: The study revealed that the serum cortisol concentration was high initially and continues to decrease to resting cortisol level at 120 min indicating that cortisol hormone is released following acute stress as a primary response in grouper juveniles.

    Matched MeSH terms: Seafood
  3. Bacteria flora and heavy metals in cultivated oysters Crassostrea iredalei of Setiu Wetland, East Coast Peninsular Malaysia
    Najiah M, Nadirah M, Lee KL, Lee SW, Wendy W, Ruhil HH, et al.
    Vet Res Commun, 2008 Jun;32(5):377-81.
    PMID: 18369732 DOI: 10.1007/s11259-008-9045-y
    Slipper oyster Crassostrea iredalei is a species of good demand for its sweet flavor and white coloured flesh. The filter feeding nature predisposes oysters to accumulation of pathogenic and heavy metals in waters impacted by sewage pollutions and may thus render the oysters unfit for human consumption. A study was undertaken to investigate the presence of bacteria flora and heavy metal concentrations in cultivated oysters Crassostrea iredalei at Setiu Wetland, Terengganu, the only source of cultivated oysters in East Coast of Malaysia. A total of 200 slipper oyster samples were analyzed. The bacteria were isolated using non selective agar such as TSA agar and selective agars before they were then identified using conventional methods in combination with BBL Crystal identification kit. Heavy metals such as zinc (Zn), copper (Cu), cadmium (Cd), and lead (Pb) concentrations were determined using atomic absorption spectrophotometry. Results showed that the oysters harbor predominantly Shewanella putrifaciens followed by Vibrio parahaemolyticus, Vibrio vulnificus, Vibrio cholerae, Enterobacter cloacae, Escherichia coli and Chromobacterium violaceum. They also contain high concentration of Zn (785.68 +/- 285.88 microg/g) with the lowest heavy metal was Pb (0.17 +/- 0.15 microg/g), whilst the concentrations of other heavy metals were Cu (38.9 +/- 13.2 microg/g) and Cd (1.60 +/- 0.28 microg/g). The study is very useful to evaluate the type of bacteria and heavy metal present in oyster meat for human consumption.
    Matched MeSH terms: Seafood/analysis*; Seafood/microbiology*
  4. Fulltext Effect of salinity on embryo and larval development of oyster Crassostrea iredalei
    Ng, Amelia Phei Fang, Teh, Chiew Peng, Poi, Khoy Yen, Tan, Aileen Shau Hwai, Zulfigar Yasin
    Trop Life Sci Res, 2016;27(11):23-29.
    MyJurnal
    The effects of salinity on the embryonic and larvae stage of Crassostrea iredalei
    were investigated. Fertilised eggs and one day old D-larvae were subjected to salinities
    ranging from 0 to 30 ppt at temperature of 30±2°C. At salinity lower than 10 ppt, 100%
    mortality was observed. For embryo development, the highest survival was observed at
    salinity 25 ppt with 80.9±2.2% survival with no significant difference compared to 15 and
    30 ppt. Shell height and length were both greatest at salinity 30 ppt. Throughout the 11
    days culture, the highest larval survival occurred at salinity 15 ppt with no significant
    difference compared to all other salinities except 10 ppt. Larval shell sizes showed no
    significant differences between salinities, except for 10 ppt. Optimum culture condition for
    larvae growth are salinities ranging from 15 to 30 ppt whereby the larval of this species
    can tolerate wider range of salinity compared to other oyster species and thus, making it a
    competitive species to be cultured.
    Matched MeSH terms: Seafood
  5. Fulltext The effects of salinity on the filtration rates of juvenile tropical oyster Cassostrea iredalei
    Chang, Geraldine Olive Ju Lien, Lai, Ven Inn, Tan, Aileen Shau Hwai, Zulfigar Yasin
    Trop Life Sci Res, 2016;27(11):45-51.
    MyJurnal
    A small scale laboratory study was conducted to determine the effects of
    salinity ranging from 15, 20, 25, 30, 35, 40, and 45 ppt on the filtration rates of juvenile
    oyster Crassostrea iredalei with 25 ppt as the control. Three juvenile oysters (shell weight:
    1.04 ± 0.12 g; shell length: 1.9 ± 0.2 cm; shell height: 1.9 ± 0.1 cm) were used to test the
    filtration rates in each salinity over the course of 8 hours. The hourly filtration rates were
    determined from the exponential decrease in algal (Chaetoceros calcitrans) concentration
    as a function of time. The oyster in 35 ppt salinity produced the highest overall filtration
    rate (FR2) with 134.06 ± 15.66 mL–1 hr–1 oyster–1 and the lowest overall filtration rate (FR2)
    occurred in oyster exposed to 15 ppt and 45 ppt with 31.30 ± 6.90 mL–1 hr–1 oyster–1 and
    32.11 ± 7.68 mL–1 hr–1 oyster–1
    respectively throughout the 8 hours. The result from this
    study can be useful for optimum oyster culturing and the oysters can be employed as a
    natural biofilter in marine polyculture farming.
    Matched MeSH terms: Seafood
  6. The Feeding Ecology of the Blue Swimming Crab, Portunus pelagicus (Linnaeus, 1758), at Kung Krabaen Bay, Chanthaburi Province, Thailand
    Kunsook C, Gajaseni N, Paphavasit N
    Trop Life Sci Res, 2014 Aug;25(1):13-27.
    PMID: 25210585
    The natural diet of blue swimming crabs, Portunus pelagicus, was investigated from October 2008 to October 2009 using hand sampling and a crab gill net. The results showed that the major prey items in the stomach contents of P. pelagicus were teleost fish (29.61%), organic matter (20.69%), crustaceans (18.3%) and shelled molluscs (11.46%). Significant differences were found in diet composition between juvenile and mature crabs, between crabs inside and outside the bay and among seasons. In contrast, significant differences were not found between male and female crabs.
    Matched MeSH terms: Seafood
  7. Fulltext Muscle Fatty Acid Content in Selected Freshwater Fish from Bukit Merah Reservoir, Perak, Malaysia
    Jaya-Ram A, Fuad F, Zakeyuddin MS, Sah ASRM
    Trop Life Sci Res, 2018 Jul;29(2):103-117.
    PMID: 30112144 MyJurnal DOI: 10.21315/tlsr2018.29.2.8
    One of the oldest reservoirs in Peninsular Malaysia, Bukit Merah Reservoir, is a place in which locals participate in fishing activities. Inland fisheries are important to individuals, society and the environment; whereby they generate a source of income and food security. It is essential to gauge the nutrition value of fish caught in this location as food source, especially in terms of fatty acid composition, to better demonstrate its potential towards the betterment of human health and general well-being. From an initial list of 47 fish species available in Bukit Merah Reservoir, a total of seven edible freshwater fish species were identified, namely tinfoil barb (Barbonymus schwanenfeldii), Javanese barb (Barbonymus gonionotus), hampala barb (Hampala macrolepidota), beardless barb (Cyclocheilichthys apogon), glassfish (Oxygaster anomalura), striped snakehead (Channa striata) and horseface loach (Acantopsis dialuzona), and muscle fatty acid content was analysed to determine their nutritional value. Muscle of cyprinid fish contained substantial amount of omega-3 long-chain polyunsaturated fatty acids (LC-PUFA) compared to fish from Channidae and Cobitidae families. Javanese and tinfoil barbs muscle recorded the highest levels of combined eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) indicating the highest nutritional value comparatively. Unexpectedly, the striped snakehead, a predatory carnivore, contained lower levels of LC-PUFA compared to detrivorous/herbivorous fishes. This further justifies that the omega-3 polyunsaturated fatty acids (PUFA) content in fish muscle varies according to their feeding habits. Even though it has been recommended that marine fish be consumed to improve health to a certain extent, there still are benefits of consuming freshwater fish, as there are several species which contain considerable amounts of beneficial omega-3 PUFA.
    Matched MeSH terms: Seafood
  8. Fulltext Identification of Fish Species and Toxins Implicated in a Snapper Food Poisoning Event in Sabah, Malaysia, 2017
    Dao HV, Uesugi A, Uchida H, Watanabe R, Matsushima R, Lim ZF, et al.
    Toxins (Basel), 2021 09 15;13(9).
    PMID: 34564661 DOI: 10.3390/toxins13090657
    In the coastal countries of Southeast Asia, fish is a staple diet and certain fish species are food delicacies to local populations or commercially important to individual communities. Although there have been several suspected cases of ciguatera fish poisoning (CFP) in Southeast Asian countries, few have been confirmed by ciguatoxins identification, resulting in limited information for the correct diagnosis of this food-borne disease. In the present study, ciguatoxin-1B (CTX-1B) in red snapper (Lutjanus bohar) implicated in a CFP case in Sabah, Malaysia, in December 2017 was determined by single-quadrupole selected ion monitoring (SIM) liquid chromatography/mass spectrometry (LC/MS). Continuous consumption of the toxic fish likely resulted in CFP, even when the toxin concentration in the fish consumed was low. The identification of the fish species was performed using the molecular characterization of the mitochondrial cytochrome c oxidase subunit I gene marker, with a phylogenetic analysis of the genus Lutjanus. This is the first report identifying the causative toxin in fish-implicated CFP in Malaysia.
    Matched MeSH terms: Seafood/classification; Seafood/toxicity*
  9. Fulltext Assessment of heavy metals contamination and human health risk in shrimp collected from different farms and rivers at Khulna-Satkhira region, Bangladesh
    Sarkar T, Alam MM, Parvin N, Fardous Z, Chowdhury AZ, Hossain S, et al.
    Toxicol Rep, 2016;3:346-350.
    PMID: 28959555 DOI: 10.1016/j.toxrep.2016.03.003
    This study is aimed to assess the heavy metals contamination and health risk in Shrimp (Macrobrachium rosenbergii and Penaeus monodon) collected from Khulna-Satkhira region in Bangladesh. The results showed that the Pb concentrations (0.52-1.16 mg/kg) in all shrimp samples of farms were higher than the recommended limit. The Cd levels (0.05-0.13 mg/kg) in all samples and Cr levels in all farms except tissue content at Satkhira farm were higher than the permissible limits. The individual concentration of Pb, Cd, and Cr between shrimp tissue and shell in all rivers and farms were not statistically significant (P > 0.05). Target hazard quotient (THQ) and hazard index (HI) were estimated to assess the non-carcinogenic health risks. Shrimp samples from all locations under the current study were found to be safe for consumption, the possibility of health risk associated with non-carcinogenic effect is very low for continuous consumption for 30 years.
    Matched MeSH terms: Seafood
  10. Fulltext Cadmium and lead in blood cockle (Anadara granosa) from Asajaya, Sarawak, Malaysia
    Hossen MF, Hamdan S, Rahman MR
    ScientificWorldJournal, 2014;2014:924360.
    PMID: 25538965 DOI: 10.1155/2014/924360
    The concentrations were ranged from 1.35 ± 0.16 to 2.22 ± 0.34 µg/g (dry weight) and 2.65 ± 0.34 to 4.36 ± 0.53 µg/g (dry weight) for Cd and Pb, respectively, in blood cockle Anadara granosa from four sites of Sabang River, namely, Kampung Sambir, Kampung Tambirat, Beliong Temple, and Kampung Tanjung Apong, which are located at Asajaya, Sarawak, Malaysia. All values exceeded safety limits set by Malaysian Food Regulation (1985). It may be the cause of serious human health problems after long term consumption. Thus, consumer should have consciousness about such type of seafood from mentioned sites and need further investigation.
    Matched MeSH terms: Seafood/analysis*
  11. Health risk assessments of heavy metal exposure via consumption of marine mussels collected from anthropogenic sites
    Yap CK, Cheng WH, Karami A, Ismail A
    Sci Total Environ, 2016 May 15;553:285-96.
    PMID: 26925739 DOI: 10.1016/j.scitotenv.2016.02.092
    A total of 40 marine mussel Perna viridis populations collected (2002-2009) from 20 geographical sites located in two busy shipping lanes namely the Straits of Malacca (10 sites; 16 populations) and the Straits of Johore (8 sites; 21 populations) and three populations (2 sites) on the east coast of Peninsular Malaysia, was determined for Cd, Cu, Fe, Ni, Pb and Zn concentrations. In comparison with the maximum permissible limits (MPLs) set by existing food safety guidelines, all metal concentrations found in all the mussel populations were lower than the prescribed MPLs. In terms of the provisional tolerable weekly intake prescribed by the Joint FAO/WHO Expert Committee on Food Additives (JECFA) and oral reference doses (ORDs) by the USEPA, all the studied metals (except for Pb) were unlikely to become the limiting factors or unlikely to pose a risk for the consumption of the mussel populations. The estimated daily intake (EDI) for average level mussel (ALM) and high level mussel (HLM) consumers of mussels was found to be lower than the ORD guidelines for Cd, Cu, Fe, Ni and Zn. Furthermore, the target hazard quotient (THQ) was found to be less than 1 for ALM consumers but higher than 1 for HLM consumers in some sites. Therefore, there were no potential human health risks to the ALM consumers of the mussels. However, for Pb THQ values, the Pb levels in some mussel populations could create a health risk problem. Present results indicate that the consumption amounts of mussels should be limited for minimizing potential health risks of heavy metals to the HLM consumers.
    Matched MeSH terms: Seafood
  12. Microplastic and mesoplastic contamination in canned sardines and sprats
    Karami A, Golieskardi A, Choo CK, Larat V, Karbalaei S, Salamatinia B
    Sci Total Environ, 2018 Jan 15;612:1380-1386.
    PMID: 28898945 DOI: 10.1016/j.scitotenv.2017.09.005
    No report was found on the occurrence of microplastics in processed seafood products that are manufactured for direct human consumption. This study investigates the potential presence of micro- and mesoplastics in 20 brands of canned sardines and sprats originating from 13 countries over 4 continents followed by their chemical composition determination using micro-Raman spectroscopy. The particles were further inspected for their inorganic composition through energy-dispersive X-ray spectroscopy (EDX). Plastic particles were absent in 16 brands while between 1 and 3 plastic particles per brand were found in the other 4 brands. The most abundant plastic polymers were polypropylene (PP) and polyethylene terephthalate (PET). The presence of micro- and mesoplastics in the canned sardines and sprats might be due to the translocation of these particles into the edible tissues, improper gutting, or the result of contamination from the canneries. The low prevalence of micro- and mesoplastics sized >149μm, and the absence of potentially hazardous inorganic elements on them, might indicate the limited health risks associated with their presence in canned sardines and sprats. Due to the possible increase in micro- and mesoplastic loads in seafood products over time, the findings of this study suggest their quantification to be included as one of the components of food safety management systems.
    Matched MeSH terms: Seafood*
  13. Fulltext Identification of major and minor allergens of black tiger prawn (Penaeus monodon) and king prawn (Penaeus latisulcatus)
    Sahabudin S, Misnan R, Yadzir ZH, Mohamad J, Abdullah N, Bakhtiar F, et al.
    Malays J Med Sci, 2011 Jul;18(3):27-32.
    PMID: 22135598 MyJurnal
    BACKGROUND: Prawns and shrimp are a frequent cause of seafood allergy mediated by IgE antibodies. Penaeus monodon and Penaeus latisulcatus, commonly known as black tiger prawn and king prawn, respectively, are among the most frequently consumed prawns in Malaysia. The aim of this study was to identify the IgE-binding proteins of these 2 prawn species.
    METHODS: Raw and boiled prawn extracts were prepared and then resolved by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). IgE-immunoblotting was then performed using sera from patients with positive skin prick tests to the raw prawn extracts.
    RESULTS: SDS-PAGE analysis of the raw extracts of both prawn species revealed 23 protein bands; the boiled extracts yielded fewer protein bands. The bands in the range of 40 to 100 kDa were sensitive to heat and therefore were not found in the boiled extracts. Immunoblot of raw extracts of black tiger prawns and king prawns yielded 14 and 11 IgE-binding proteins, respectively, with molecular weights of between 15 and 200 kDa. Proteins at 36, 42, and 49 kDa were detected as the major allergens in both species of prawns. A protein of 75 kDa was also identified as a major allergen in black tiger prawns. Other potential allergens were also observed at various molecular masses.
    CONCLUSION: Proteins of 36, 42, and 49 kDa were identified as the major allergens of both species of prawns. The 36 and 42 kDa proteins are hypothesised to be tropomyosin and arginine kinase, respectively. A high molecular weight protein of 75 kDa was found to be an additional major allergen in black tiger prawns.
    KEYWORDS: Penaeus; allergens; allergy and clinical immunology; hypersensitivity; immunoblotting; tropomyosin
    Matched MeSH terms: Seafood
  14. Medics and Marine Mammals - An Unlikely but Important Connection for Humanity's Survival
    Ponnampalam LS
    Malays J Med Sci, 2014 Mar;21(2):1-3.
    PMID: 24876801
    Marine mammals, as top predators in the marine food web, are sentinels of changes in the oceans and public health. Pollution in the sea and overfishing of seafood resources affects these organisms just as much as it affects human beings. Medics, especially doctors, have an influential reach to patients, and are in an ideal position to get better acquainted with ongoing marine environmental issues and subsequently disseminating such information to them. While seemingly an out-of-the-box approach, it is one that can help with environmental conservation and preservation for the future of humanity.
    Matched MeSH terms: Seafood
  15. Comparative PCR-based fingerprinting of Vibrio cholerae isolated in Malaysia
    Shuan Ju Teh C, Thong KL, Osawa R, Heng Chua K
    J Gen Appl Microbiol, 2011;57(1):19-26.
    PMID: 21478644
    Vibrio cholerae, the causative agent of cholera, is endemic in many parts of the world, especially in countries poor in resources. Molecular subtyping of V. cholerae is useful to trace the regional spread of a clone or multidrug-resistant strains during outbreaks of cholera. Current available PCR-based fingerprinting methods such as Random Amplified Polymorphic DNA (RAPD)-PCR, Enterobacterial Repetitive Intergenic Consensus Sequence (ERIC)-PCR, and Repetitive Extragenic Palindromic (REP)-PCR were used to subtype V. cholerae. However, there are problems for inter-laboratory comparison as these PCR methods have their own limitations especially when different PCR methods have been used for molecular typing. In this study, a Vibrio cholerae Repeats-PCR (VCR-PCR) approach which targets the genetic polymorphism of the integron island of Vibrios was used and compared with other PCR-based fingerprinting methods in subtyping. Forty-three V. cholerae of different serogroups from various sources were tested. The PCR-fingerprinting approaches were evaluated on typeability, reproducibility, stability and discriminatory power. Overall, Malaysian non-O1/non-O139 V. cholerae were more diverse than O1 strains. Four non-O1/non-O139 strains were closely related with O1 strains. The O139 strain in this study shared similarity with strains of both O1 and non-O1/non-O139 serogroups. ERIC-PCR was the most discriminative approach (D value = 0.996). VCR-PCR was useful in discriminating non-O1/non-O139 strains. RAPD-PCR and REP-PCR were less suitable for efficient subtyping purposes as they were not reproducible and lacked stability. The combination of the ERIC-PCR and VCR-PCR may overcome the inadequacy of any one approach and hence provide more informative data.
    Matched MeSH terms: Seafood/microbiology
  16. Molecular characterization of serogrouping and virulence genes of Malaysian Vibrio cholerae isolated from different sources
    Shuan Ju Teh C, Lin Thong K, Tein Ngoi S, Ahmad N, Balakrish Nair G, Ramamurthy T
    J Gen Appl Microbiol, 2009 Dec;55(6):419-25.
    PMID: 20118606
    A pair of primers targeting the hlyA gene for Vibrio cholerae which could distinguish the classical from El Tor biotypes was designed and combined with other specific primers for ompW, rfb complex, and virulence genes such as ctxA, toxR, and tcpI in a multiplex PCR (m-PCR) assay. This m-PCR correctly identified 39 V. cholerae from clinical, water and seafood samples. The efficiency of this multiplex PCR (m-PCR) was compared with conventional biochemical and serogrouping methods. One O139 and 25 O1 V. cholerae strains including 10 environmental strains harbored all virulence-associated genes except 1 clinical strain which only had toxR and hlyA genes. Thirteen environmental strains were classified as non-O1/non-O139 and had the toxR and hlyA genes only. The detection limit of m-PCR was 7 x 10(4) cfu/ml. The m-PCR test was reliable and rapid and reduced the identification time to 4 h.
    Matched MeSH terms: Seafood/microbiology*
  17. Migratory Fish Bone Presented With Extensive Surgical Subcutaneous Emphysema: A Case Report
    Muhammad A, Tuang GJ, Zahedi FD, Nik Hussin NR
    J Emerg Med, 2021 Jul;61(1):e4-e6.
    PMID: 33863569 DOI: 10.1016/j.jemermed.2021.02.022
    BACKGROUND: Fishbone ingestion represents a common cause for emergency department (ED) referral. In the majority of cases, an observed fishbone can be easily retrieved in the clinic setting. An impacted fishbone in the throat, albeit uncommon, carries potential risks of life-threatening events. Unusual complications caused by a migrated fishbone, including deep neck abscess, airway obstruction, and major vessels injury, are greatly influenced by the type of ingested fishbone and time between onset and presentation.

    CASE REPORT: Here we report an unusual case of surgical subcutaneous emphysema after multiple attempts of purging to remove an ingested fishbone. WHY SHOULD AN EMERGENCY PHYSICIAN BE AWARE OF THIS?: Fishbone foreign body is a common presentation to the ED. A thorough history and examination for the migratory foreign body is essential, as the complications are consequential.

    Matched MeSH terms: Seafood
  18. Anisakiasis causing acute dysentery in Malaysia
    Amir A, Ngui R, Ismail WH, Wong KT, Ong JS, Lim YA, et al.
    Am J Trop Med Hyg, 2016 Aug 03;95(2):410-2.
    PMID: 27325803 DOI: 10.4269/ajtmh.16-0007
    Human anisakiasis is a zoonosis acquired by eating raw or undercooked infected seafood. Herein, we report a case of acute dysentery caused by anisakiasis in a 64-year-old man in Malaysia. A colonoscopy was performed and a nematode larva was found penetrating the mucosa of the ascending colon. Bleeding was observed at the site of penetration. Y-shaped lateral epidermal cords were seen from the cross section of the worm, which is a prominent feature of Anisakis larva. Molecular analysis using polymerase chain reaction of cytochrome oxidase 2 (cox2) gene confirmed the specimen to be larva of Anisakis simplex.
    Matched MeSH terms: Seafood/parasitology
  19. Sennetsu neorickettsiosis: a probable fish-borne cause of fever rediscovered in Laos
    Newton PN, Rolain JM, Rasachak B, Mayxay M, Vathanatham K, Seng P, et al.
    Am J Trop Med Hyg, 2009 Aug;81(2):190-4.
    PMID: 19635868
    Neorickettsia sennetsu has been described from Japan and Malaysia, causing a largely forgotten infectious mononucleosis-like disease. Because it is believed to be contracted from eating raw fish, frequently consumed in the Lao PDR, we looked for evidence of N. sennetsu among Lao patients and fish. A buffy coat from 1 of 91 patients with undifferentiated fever was positive by 16S rRNA amplification and sequencing and real-time polymerase chain reactions (PCR) targeting two N. sennetsu genes. Lao blood donors and patients with fever, hepatitis, or jaundice (N = 1,132) had a high prevalence (17%) of immunofluorescence assay IgG anti-N. sennetsu antibodies compared with 4% and 0% from febrile patients (N = 848) in Thailand and Malaysia, respectively. We found N. sennetsu DNA by PCR, for the first time, in a fish (Anabas testudineus). These data suggest that sennetsu may be an under-recognized cause of fever and are consistent with the hypothesis that it may be contracted from eating raw fish.
    Matched MeSH terms: Seafood/microbiology*
  20. Fulltext A Colorimetric pH Sensor Based on Clitoria sp and Brassica sp for Monitoring of Food Spoilage Using Chromametry
    Ahmad NA, Yook Heng L, Salam F, Mat Zaid MH, Abu Hanifah S
    Sensors (Basel), 2019 Nov 05;19(21).
    PMID: 31694284 DOI: 10.3390/s19214813
    A developed colorimetric pH sensor film based on edible materials for real-time monitoring of food freshness is described. The mixed natural dyes from edible plants Clitoria sp and Brassica sp were extracted and incorporated into ι-carrageenan film as a colorimetric pH sensor film for monitoring food spoilage and its freshness. The color changes of the developed colorimetric sensor film were measured with chromametry and UV-vis spectroscopy, respectively. Experimental results show that colorimetric pH sensor film demonstrated statistically significant differences (p < 0.05) between CIE-L*a*b* coordinates color system indicated that the developed colorimetric sensor film was able to give a gradual change in color over a wide pH range. The color of the colorimetric sensor film also changes discretely and linearly with factors that contribute to food spoilage using shrimp and durian samples. Moreover, the developed colorimetric pH sensor film has the potential to be used as a safe, non-destructive testing and also a flexibly visual method for direct assessment of food freshness indicator during storage.
    Matched MeSH terms: Seafood
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