Displaying publications 1 - 20 of 543 in total

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  1. Cherenet T, Sani RA, Panandam JM, Nadzr S, Speybroeck N, van den Bossche P
    Onderstepoort J Vet Res, 2004 Dec;71(4):307-12.
    PMID: 15732457
    During a period of four consecutive years, trypanosomosis surveys were conducted in a tsetse-infested and tsetse-free area of the Amhara Region of north-west Ethiopia. In each study area randomly selected communal cattle were sampled and their blood was investigated using parasitological diagnostic methods. At the same time the population of biting flies was sampled. The monthly average prevalence of trypanosome infections in cattle did not differ significantly between study areas. In both study areas, the prevalence of trypanosome infections was highest during the long rainy season. Trypanosome infections were mainly due to Trypanosoma vivax and they significantly reduced the average packed cell volume and the body condition of the animals. The monthly prevalence of infection was correlated with the density of biting flies, such as Tabanidae and Stomoxys spp., in the preceding month suggesting an important role of mechanical transmission in the epidemiology of trypanosomosis in both areas.
    Matched MeSH terms: Seasons
  2. Ekker MS, Jacob MA, van Dongen MME, Aarnio K, Annamalai AK, Arauz A, et al.
    BMJ Open, 2019 Nov 14;9(11):e031144.
    PMID: 31727655 DOI: 10.1136/bmjopen-2019-031144
    INTRODUCTION: Worldwide, 2 million patients aged 18-50 years suffer a stroke each year, and this number is increasing. Knowledge about global distribution of risk factors and aetiologies, and information about prognosis and optimal secondary prevention in young stroke patients are limited. This limits evidence-based treatment and hampers the provision of appropriate information regarding the causes of stroke, risk factors and prognosis of young stroke patients.

    METHODS AND ANALYSIS: The Global Outcome Assessment Life-long after stroke in young adults (GOAL) initiative aims to perform a global individual patient data meta-analysis with existing data from young stroke cohorts worldwide. All patients aged 18-50 years with ischaemic stroke or intracerebral haemorrhage will be included. Outcomes will be the distribution of stroke aetiology and (vascular) risk factors, functional outcome after stroke, risk of recurrent vascular events and death and finally the use of secondary prevention. Subgroup analyses will be made based on age, gender, aetiology, ethnicity and climate of residence.

    ETHICS AND DISSEMINATION: Ethical approval for the GOAL study has already been obtained from the Medical Review Ethics Committee region Arnhem-Nijmegen. Additionally and when necessary, approval will also be obtained from national or local institutional review boards in the participating centres. When needed, a standardised data transfer agreement will be provided for participating centres. We plan dissemination of our results in peer-reviewed international scientific journals and through conference presentations. We expect that the results of this unique study will lead to better understanding of worldwide differences in risk factors, causes and outcome of young stroke patients.

    Matched MeSH terms: Seasons
  3. Jaswir I, Shahidan N, Othman R, Has-Yun Hashim YZ, Octavianti F, bin Salleh MN
    J Oleo Sci, 2014;63(8):761-7.
    PMID: 25007748
    Carotenoids are antioxidants with pharmaceutical potential. The major carotenoids important to humans are α-carotene, β-carotene, lycopene, lutein, zeaxanthin, and β-cryptoxanthin. Some of the biological functions and actions of these individual carotenoids are quite similar to each other, whereas others are specific. Besides genotype and location, other environmental effects such as temperature, light, mineral uptake, and pH have been found affect carotenoid development in plant tissues and organs. Therefore, this research investigated the effects of the season and storage periods during postharvest handling on the accumulation of carotenoid in pumpkin. This study shows that long-term storage of pumpkins resulted in the accumulation of lutein and β-carotene with a slight decrease in zeaxanthin. The amounts of β-carotene ranged from 174.583±2.105 mg/100g to 692.871±22.019 mg/100g, lutein from 19.841±9.693 mg/100g to 59.481±1.645 mg/100g, and zeaxanthin from not detected to 2.709±0.118 mg/100g. The pumpkins were collected three times in a year; they differed in that zeaxanthin was present only in the first season, while the amounts of β-carotene and lutein were the highest in the second and third seasons, respectively. By identifying the key factors among the postharvest handling conditions that control specific carotenoid accumulations, a greater understanding of how to enhance the nutritional values of pumpkin and other crops will be gained. Postharvest storage conditions can markedly enhance and influence the levels of zeaxanthin, lutein, and β-carotene in pumpkin. This study describes how the magnitudes of these effects depend on the storage period and season.
    Matched MeSH terms: Seasons*
  4. Cardosa MJ, Choo BH, Zuraini I
    PMID: 1667957
    This study describes the status of viral encephalitis in Perak, Malaysia during the year 1990. In addition, 14 cases selected from Penang and Perak during the years 1989 and 1990 are presented, with data showing titers of neutralizing antibodies against Japanese encephalitis virus (JEV) and dengue 2 virus, titers of antibodies against JEV and dengue virus antigens as determined by DEIA, and a comparison of these with the presence of IgM to JEV and dengue virus. These data show that there probably is far more viral encephalitis due to JEV in Malaysia than the national figures reflect.
    Matched MeSH terms: Seasons
  5. Graham LE, Knack JJ, Graham ME, Graham JM, Zulkifly S
    J Phycol, 2015 Jun;51(3):408-18.
    PMID: 26986658 DOI: 10.1111/jpy.12296
    Periphyton dominated by the cellulose-rich filamentous green alga Cladophora forms conspicuous growths along rocky marine and freshwater shorelines worldwide, providing habitat for diverse epibionts. Bacterial epibionts have been inferred to display diverse functions of biogeochemical significance: N-fixation and other redox reactions, phosphorus accumulation, and organic degradation. Here, we report taxonomic diversity of eukaryotic and prokaryotic epibionts and diversity of genes associated with materials cycling in a Cladophora metagenome sampled from Lake Mendota, Dane Co., WI, USA, during the growing season of 2012. A total of 1,060 distinct 16S, 173 18S, and 351 28S rRNA operational taxonomic units, from which >220 genera or species of bacteria (~60), protists (~80), fungi (6), and microscopic metazoa (~80), were distinguished with the use of reference databases. We inferred the presence of several algal taxa generally associated with marine systems and detected Jaoa, a freshwater periphytic ulvophyte previously thought endemic to China. We identified six distinct nifH gene sequences marking nitrogen fixation, >25 bacterial and eukaryotic cellulases relevant to sedimentary C-cycling and technological applications, and genes encoding enzymes in aerobic and anaerobic pathways for vitamin B12 biosynthesis. These results emphasize the importance of Cladophora in providing habitat for microscopic metazoa, fungi, protists, and bacteria that are often inconspicuous, yet play important roles in ecosystem biogeochemistry.
    Matched MeSH terms: Seasons
  6. Maryam S, Fadzly N, Amirul AA, Zuharah WF
    Rev Inst Med Trop Sao Paulo, 2017 Apr 03;59:e4.
    PMID: 28380115 DOI: 10.1590/S1678-9946201759004
    Paederus fuscipes, a vector of Paederus dermatitis in most tropical and subtropical countries of the world have a high prevalence in human dwellings due to their positively phototaxic behaviour which has caused a tremendous impact on human health. In this paper, P. fuscipes dispersal flights were studied for two seasons of the rice cultivation phases in residential premises built close to rice field areas (≈32-60 m and 164 m) in mainland Penang, Malaysia. We examined the effects of different light illuminance, building floor level and their association with rice stages as a focal cause of P. fuscipes dispersion from the rice fields towards human dwellings. The present study showed a significant interaction between different light illuminances and rice cultivation phases in attracting P. fuscipes to disperse and invade human dwellings. The highest number of P. fuscipes was captured near the bright light. P. fuscipes flights increased in line with each floor level, and the highest captures took place at higher building floor levels (levels 2 and 3) compared to lower building floor levels (ground floor and level 1) of a three storey apartment in both rice seasons. This finding not only conveys a better understanding on P. fuscipes dispersal pattern, but also draws public attention on the occurrence of dermatitis linearis caused by the Paederus beetles.
    Matched MeSH terms: Seasons
  7. Mohamad N, Zuharah WF
    Trop Biomed, 2014 Mar;31(1):166-73.
    PMID: 24862057 MyJurnal
    Toxorhynchites splendens larvae are a natural predator of dengue vector mosquito larvae, Aedes albopictus. This study was carried out to evaluate the predation rate of Tx. splendens third instar larvae on Ae. albopictus larvae in 24 h. Each predator was offered prey at a density between 10 to 50 individuals. Predation rate of Tx. splendens were also tested with two manipulated factors; various types of container and different water volumes. The experiment was evaluated in man-made containers (tin cans, plastic drinking glasses and rubber tires) and natural container (bamboo stumps) which were filled with different water volumes (full, half full, 1/4 full, and 1/8 full). The prey density and the characteristics of the container were found as significant factors which influence the predation rate of Tx. splendens. The predator consumed significantly more prey at higher prey densities (40 and 50 preys) compared to the lowest density (10 preys) (F=3.935, df=4, p=0.008). The results showed significantly higher consumption in horizontal shaped container of rubber tire than in vertical shape of bamboo stumps (F=3.100, df=3, p=0.029). However, the water volume had no significant effect on predation rate of Tx. splendens (F=1.736, df=3, p=0.162). We generally suggest that Tx. splendens is best to be released in discarded tires or any other containers with horizontal shape design with wide opening since Tx. splendens can become more effective in searching prey in this type of container design. This predator is also a suitable biocontrol candidates to be introduced either in wet and dry seasons in Malaysia.
    Matched MeSH terms: Seasons
  8. Kulthanan K, Chusakul S, Recto MT, Gabriel MT, Aw DCW, Prepageran N, et al.
    Allergy Asthma Immunol Res, 2018 Jul;10(4):370-378.
    PMID: 29949833 DOI: 10.4168/aair.2018.10.4.370
    PURPOSE: Across Hong Kong, Malaysia, the Philippines, Singapore, Thailand and Vietnam, (referred to as Asia) approximately 30-53 million individuals of the 151 million employed suffer from allergic rhinitis (AR) and urticaria. It is estimated that approximately 90% of patients with these allergic conditions are insufficiently treated, impacting the socioeconomic burden in terms of absence from work and decreased productivity. This study aims to estimate the socioeconomic burden of allergies in Asia and the cost savings that their adequate management can provide. Due to the limited availability of regional data, this study focused AR and urticaria in selected countries.

    METHODS: Published literature, information from statistical bureaus, clinician surveys and extrapolation of selected data from the European Union were used to determine the socioeconomic costs of AR and urticaria.

    RESULTS: Many patients in Asia suffer from perennial allergies and experience symptoms of AR and urticaria for up to 298 days per year. An estimate of the indirect costs of patients insufficiently treated for AR and urticaria amounts to USD 105.4 billion a year, which equates to USD 1,137-2,195 per patient due to absenteeism and presenteeism. Adherence to guideline-approved treatment can lead to estimated savings of up to USD 104 billion.

    CONCLUSIONS: The current study suggests that within Asia, the socioeconomic impact of AR and urticaria is similar to that seen in the European Union in spite of the lower wages in Asia. This is due to the mainly perennial allergens prevailing in Asia, whereas the sensitization patterns observed in the European Union are dominated by seasonal exposure to pollen. These results underline the need for governmental initiatives to increase public awareness on the prevention and treatment of these and other allergic diseases as well as greater research funding and large-scale studies to reduce their growing socioeconomic burden in coming years.

    Matched MeSH terms: Seasons
  9. Browne N, Braoun C, McIlwain J, Nagarajan R, Zinke J
    PeerJ, 2019;7:e7382.
    PMID: 31428541 DOI: 10.7717/peerj.7382
    For reefs in South East Asia the synergistic effects of rapid land development, insufficient environmental policies and a lack of enforcement has led to poor water quality and compromised coral health from increased sediment and pollution. Those inshore turbid coral reefs, subject to significant sediment inputs, may also inherit some resilience to the effects of thermal stress and coral bleaching. We studied the inshore turbid reefs near Miri, in northwest Borneo through a comprehensive assessment of coral cover and health in addition to quantifying sediment-related parameters. Although Miri's Reefs had comparatively low coral species diversity, dominated by massive and encrusting forms of Diploastrea, Porites, Montipora, Favites, Dipsastrea and Pachyseris, they were characterized by a healthy cover ranging from 22 to 39%. We found a strong inshore to offshore gradient in hard coral cover, diversity and community composition as a direct result of spatial differences in sediment at distances <10 km. As well as distance to shore, we included other environmental variables like reef depth and sediment trap accumulation and particle size that explained 62.5% of variation in benthic composition among sites. Miri's reefs showed little evidence of coral disease and relatively low prevalence of compromised health signs including bleaching (6.7%), bioerosion (6.6%), pigmentation response (2.2%), scars (1.1%) and excessive mucus production (0.5%). Tagged colonies of Diploastrea and Pachyseris suffering partial bleaching in 2016 had fully (90-100%) recovered the following year. There were, however, seasonal differences in bioerosion rates, which increased five-fold after the 2017 wet season. Differences in measures of coral physiology, like that of symbiont density and chlorophyll a for Montipora, Pachyseris and Acropora, were not detected among sites. We conclude that Miri's reefs may be in a temporally stable state given minimal recently dead coral and a limited decline in coral cover over the last two decades. This study provides further evidence that turbid coral reefs exposed to seasonally elevated sediment loads can exhibit relatively high coral cover and be resilient to disease and elevated sea surface temperatures.
    Matched MeSH terms: Seasons
  10. Atif A. B., Halim-Fikri A H, Zilfalil BA
    MyJurnal
    In the human genome, point variations are most common (Nachman & Crowell, 2000) and well understood. These variations, when existing in more than 1% of the population, is referred to as
    Single Nucleotide Polymorphism (SNP) and can fall in the coding region of a gene, non coding region or intergenic regions.
    Matched MeSH terms: Seasons
  11. Xomphoutheb T, Jiao S, Guo X, Mabagala FS, Sui B, Wang H, et al.
    Sci Rep, 2020 04 20;10(1):6574.
    PMID: 32313140 DOI: 10.1038/s41598-020-63567-7
    An appropriate tillage method must be implemented by maize growers to improve phosphorus dynamics in the soil in order to increase phosphorus uptake by plant. The objective of this study was to investigate the effects of tillage systems on phosphorus and its fractions in rhizosphere and non-rhizosphere soils under maize. An experimental field was established, with phosphate fertilizers applied to four treatment plots: continuous rotary tillage (CR), continuous no-tillage (CN), plowing-rotary tillage (PR), and plowing-no tillage (PN). Under the different tillage methods, the available P was increased in the non-rhizosphere region. However, the concentration of available P was reduced in the rhizosphere soil region. The soil available P decreased with the age of the crop until the maize reached physiological maturity. The non-rhizosphere region had 132.9%, 82.5%, 259.8%, and 148.4% more available P than the rhizosphere region under the CR, PR, CN, and PN treatments, respectively. The continuous no-tillage method (CN) improved the uptake of soil phosphate by maize. The concentrations of Ca2-P, Ca8-P, Fe-P, Al-P and O-P at the maturity stage were significantly lower than other seedling stages. However, there was no significant relationship between total P and the P fractions. Therefore, a continuous no-tillage method (CN) can be used by farmers to improve phosphorus availability for spring maize. Soil management practices minimizing soil disturbance can be used to impove phosphorus availability for maize roots, increase alkaline phosphatase activity in the rhizosphere soil and increase the abundance of different phosphorus fractions.
    Matched MeSH terms: Seasons
  12. Hussain H, Yusoff MK, Ramli MF, Abd Latif P, Juahir H, Zawawi MA
    Pak J Biol Sci, 2013 Nov 15;16(22):1524-30.
    PMID: 24511695
    Nitrate-nitrogen leaching from agricultural areas is a major cause for groundwater pollution. Polluted groundwater with high levels of nitrate is hazardous and cause adverse health effects. Human consumption of water with elevated levels of NO3-N has been linked to the infant disorder methemoglobinemia and also to non-Hodgkin's disease lymphoma in adults. This research aims to study the temporal patterns and source apportionment of nitrate-nitrogen leaching in a paddy soil at Ladang Merdeka Ismail Mulong in Kelantan, Malaysia. The complex data matrix (128 x 16) of nitrate-nitrogen parameters was subjected to multivariate analysis mainly Principal Component Analysis (PCA) and Discriminant Analysis (DA). PCA extracted four principal components from this data set which explained 86.4% of the total variance. The most important contributors were soil physical properties confirmed using Alyuda Forecaster software (R2 = 0.98). Discriminant analysis was used to evaluate the temporal variation in soil nitrate-nitrogen on leaching process. Discriminant analysis gave four parameters (hydraulic head, evapotranspiration, rainfall and temperature) contributing more than 98% correct assignments in temporal analysis. DA allowed reduction in dimensionality of the large data set which defines the four operating parameters most efficient and economical to be monitored for temporal variations. This knowledge is important so as to protect the precious groundwater from contamination with nitrate.
    Matched MeSH terms: Seasons*
  13. Akbar John B, Kamaruzzaman B, Jalal K, Zaleha K
    Sains Malaysiana, 2012;41:855-861.
    The first time report on the feeding ecology and food preference of mangrove horseshoe crab Carcinoscorpius rotundicauda (Latreille 1802) at their nesting grounds along the Pahang coast is given. Monthly sampling was carried out between March 2010 and February 2011 covering both monsoonal (March to October) and non-monsoonal (November to February) seasons. Major macrobenthic gut contents (bivalves, gastropods, crustaceans, polychaetes and miscellaneous food items including plant materials) were identified using microscopic examination. An electivity index (E1) was calculated for the frequent food items observed in the gut region of C. rotundicauda during monsoon and non-monsoon seasons. The EI was negative for crustaceans and positive for all the other food items including bivalves, gastropods, polychaetes and miscellaneous food items (which include insects, amphipods, Isopods, larval and juvenile stages of fishes, foraminifera and other Annelid worms). It is interesting to note that C. rotundicauda prefered less number of bivalves than polychaetes during non monsoon seasons but it was the reverse during monsoonal period. Male crabs intensely preyed on gastropods and female prefers polychaete worms during the peak mating/nesting season (June - August 2010). Seasonal variations in food composition showed that mollusks formed the main item especially gastropods. Unidentified organic matters in the gut content analysis of C. rotundicauda showed high preference towards plant materials. Gastro Somatic Index (GaSI) analysis showed that the feeding intensity of male crabs was higher during non-monsoon period while it was higher during monsoonal period in female crabs. In conclusion, the feeding ecology of mangrove horseshoe crabs were more similar to its closer and distant conspecifics. However, it was postulated that the higher preference of polychaete worms by the female C. rotundicauda during the peak mating season indicated its role in regulating the nesting behaviour.
    Matched MeSH terms: Seasons
  14. Sharip Z, Zaki AT
    Environ Monit Assess, 2014 Aug;186(8):4959-69.
    PMID: 24696283 DOI: 10.1007/s10661-014-3751-4
    Thermal structure and water quality in a large and shallow lake in Malaysia were studied between January 2012 and June 2013 in order to understand variations in relation to water level fluctuations and in-stream mining activities. Environmental variables, namely temperature, turbidity, dissolved oxygen, pH, electrical conductivity, chlorophyll-A and transparency, were measured using a multi-parameter probe and a Secchi disk. Measurements of environmental variables were performed at 0.1 m intervals from the surface to the bottom of the lake during the dry and wet seasons. High water level and strong solar radiation increased temperature stratification. River discharges during the wet season, and unsustainable sand mining activities led to an increased turbidity exceeding 100 NTU, and reduced transparency, which changed the temperature variation and subsequently altered the water quality pattern.
    Matched MeSH terms: Seasons
  15. Alsalahi MA, Latif MT, Ali MM, Dominick D, Khan MF, Mustaffa NI, et al.
    Mar Pollut Bull, 2015 Apr 15;93(1-2):278-83.
    PMID: 25682566 DOI: 10.1016/j.marpolbul.2015.01.011
    This study aims to determine the concentration of sterols used as biomarkers in the surface microlayer (SML) in estuarine areas of the Selangor River, Malaysia. Samples were collected during different seasons through the use of a rotation drum. The analysis of sterols was performed using gas chromatography equipped with a flame ionisation detector (GC-FID). The results showed that the concentrations of total sterols in the SML ranged from 107.06 to 505.55 ng L(-1). The total sterol concentration was found to be higher in the wet season. Cholesterol was found to be the most abundant sterols component in the SML. The diagnostic ratios of sterols show the influence of natural sources and waste on the contribution of sterols in the SML. Further analysis, using principal component analysis (PCA), showed distinct inputs of sterols derived from human activity (40.58%), terrigenous and plant inputs (22.59%) as well as phytoplankton and marine inputs (17.35%).
    Matched MeSH terms: Seasons
  16. Sadrolhosseini AR, Moksin MM, Nang HL, Norozi M, Yunus WM, Zakaria A
    Int J Mol Sci, 2011;12(4):2100-11.
    PMID: 21731429 DOI: 10.3390/ijms12042100
    In this study, optical and thermal properties of normal grade and winter grade palm oil biodiesel were investigated. Surface Plasmon Resonance and Photopyroelectric technique were used to evaluate the samples. The dispersion curve and thermal diffusivity were obtained. Consequently, the variation of refractive index, as a function of wavelength in normal grade biodiesel is faster than winter grade palm oil biodiesel, and the thermal diffusivity of winter grade biodiesel is higher than the thermal diffusivity of normal grade biodiesel. This is attributed to the higher palmitic acid C(16:0) content in normal grade than in winter grade palm oil biodiesel.
    Matched MeSH terms: Seasons
  17. Bong LJ, Zairi J
    Trop Biomed, 2010 Aug;27(2):317-25.
    PMID: 20962731 MyJurnal
    House flies were collected from April 2007-April 2008 from two poultry farms (Balik Pulau and Juru) in the state of Penang. The resistance level of the first generation offspring was evaluated against DDT, malathion, propoxur, and permethrin using the topical application method. The resistance ratio (RR) of the Balik Pulau strain house flies for propoxur, malathion and DDT ranged from 10.28 to 99.00, 7.83 to 47.01 and 6.05 to 31.10, respectively. Resistance to propoxur and malathion in house fly was attributed to cross resistance to organophosphate insecticides used in the farm. Increased metabolic detoxification might be the mechanism involved in DDT resistance due to excessive application of cypermethrin formulation. The RR of the Juru strain for propoxur, malathion and DDT was in a decreasing pattern throughout the study period, ranging from 5.58 to 83.38, 15.19 to 27.82, and 10.04 to 22.69, respectively. Permethrin appeared to be the most potent insecticide in controlling house fly in both the Balik Pulau (RR=0.50 to 1.96) and Juru poultry farms (RR=0.64 to 2.40). The fluctuations of insecticides resistance in house fly was also found to correlate with climatic factors due to its rapid breeding. Relative humidity exhibited positive correlation indices with the changes in the resistance level for DDT (r=0.481, p<0.05), malathion (r=0.698, p<0.01), and permethrin (r=0.580, p<0.05) in Balik Pulau. Similarly, relative humidity in Juru also showed strong correlation with the RR for DDT (r=0.900, p<0.01), malathion (r=0.762, p<0.05), permethrin (r=0.760, p<0.05), and propoxur (r=0.897, p<0.01).
    Matched MeSH terms: Seasons
  18. Asnor Azrin Sabuti, Che Abd Rahim Mohamed, Zaharudin Ahmad
    MyJurnal
    Various environmental samples (seawater, TSS, sediment, rainwater and fly ash) from eight different stations near Kapar coastal area were analyzed. The 210 Po activity concentrations in liquid samples (seawater and rainwater) varied between 0.34 ± 0.03 mBq L-1 to 22.44 ± 0.53 mBq L-1 . Whereas the concentrations in particulate samples (TSS, sediment and fly ash) varied between 43.79 ± 2.31 Bqkg-1 to 364.48 ± 5.43 Bqkg-1 . Results also showed the radioactivity in Kapar coastal is higher than most of Malaysian coast, reaching a factor of seven. This condition is mainly due to the operation of a coal-fired power plant nearby. This study also clarify the variability of 210 Po in environment was strongly influenced from rainfall events especially during wet seasons.
    Matched MeSH terms: Seasons
  19. Arshad A, Amani AA, Amin SMN, Yusoff FM
    J Environ Biol, 2016 07;37(4 Spec No):709-13.
    PMID: 28779730
    Parapenaeopsis sculptilis (Heller, 1862) locally referred to as ?udang kulit keras? in Malaysia has profound biological, ecological, aquacultural and conservational significance. The reproductive biology of this important penaeid from the coastal waters of Perak, Peninsular Malaysia, was studied during the period between February 2012 to January 2013. Females outnumbered males with a sex ratio of M: F= 1:3 (P < 0.05). Four maturity stages of female gonads viz., immature, maturing, mature and spent stages were distinguished. The first stage of sexual maturity was attained at a length of 9.3 cm, and female P. sculptilis showed a peak gonadosomatic index during the month of April, August and October, indicating that P. sculptilis potentially breeds throughout the year. The findings of this study would greatly contribute towards the understanding of gonadal maturation, spawning season and breeding biology, which could be important for the effective population management of this prawn species.
    Matched MeSH terms: Seasons
  20. Norshazila, S., Irwandi, J., Othman, R., Yumi Zuhanis, H.H.
    MyJurnal
    Characterization and quantification of carotenoid compound is complicated, costly and timeconsuming. The accuracy and reliability of the data depend solely on the standard and to High Performance Liquid Chromatography (HPLC) analysis but the major constraint is to acquire and to maintain the pure standards. Carotenoid standards are commercially available but they are expensive and are prone to isomerization and oxidation. Thus, the purpose of this study is to establish an analytical method for isolating β-carotene by using open column chromatography (OCC) from pumpkin (Cucurbita moschata) to be used as one of the carotenoid standards for determination of total and individual carotenoid. Pumpkin with orange flesh has been chosen due to the non-seasonal nature and its availability all year-round. This study demonstrated that the purity of β-carotene standard; determined by HPLC was ranged from 92.21 to 97.95%. The standard curves with five different concentrations of β-carotene extract from pumpkins in triplicate were constructed by plotting the peak area against the concentration. The coefficient of correlation was 0.9936. Therefore, this study established that pumpkin can be a reliable source of beta-carotene standard as it is cheap and commonly available throughout the year.
    Matched MeSH terms: Seasons
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