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  1. AL-Bayaty, F.H., Omar Emad Ibrahim, William, C., Khairuddin, N.A.
    Compendium of Oral Science, 2018;5(1):26-36.
    MyJurnal
    Objective: This retrospective study aims to evaluate the possible effects of antihypertensive drugs on alveolar bone loss in patients with chronic periodontitis. Methods: 50 patients on antihypertensive drugs selected as the experimental group and 50 patients with chronic periodontitis with no known systemic illnesses as control group were randomly selected as the study samples. Orthopantomographs were obtained, calibration and assessment of alveolar bone loss was performed by using the computer software program available in the faculty, through radiographic linear measurement procedure. Premolars, first and second molars of both maxilla and mandible were measured from the most apical point to the cementoenamel junction for mesial and distal aspects in the form of millimetres and percentile of the root length. Data was statistically analyzed using independent t-test and Analysis of Covariance in SPSS Version 23 with significance at P-value, p
    Matched MeSH terms: Tooth Cervix
  2. Ab Halim N, Esa R, Chew HP
    BMC Oral Health, 2018 01 12;18(1):11.
    PMID: 29329566 DOI: 10.1186/s12903-017-0451-9
    BACKGROUND: The objective of this study was to determine the prevalence and severity of general tooth wear (GTW), i.e. tooth wear irrespective of etiology and erosive tooth wear (ETW), i.e. tooth wear predominantly due to erosion; and also to investigate the relationship between ETW and dental caries experience in 16-year-old adolescents in Kuantan, Malaysia.

    METHODS: A multi-staged cluster sampling method was employed. A total of 598 16-year-old adolescents participated in this study. Participants' demographic profile was assessed through a self-administered questionnaire. Clinical examinations were carried out under standardized conditions by a single examiner. The level of GTW was recorded using the modified Smith and Knight's Tooth Wear Index (TWI) whilst ETW were recorded using the Basic Erosive Wear Examination (BEWE) index. This index was developed to record clinical findings and assist in the decision-making process for the management of erosive tooth wear. Dental caries was recorded using the D3MFT index whereby D3 denotes obvious dental decay into dentine detected visually.

    RESULTS: The prevalence of GTW, ETW and dental caries, i.e. percentage of individuals found to have at least one lesion, was 99.8%, 45.0% and 27.8% respectively. Two thirds of affected teeth with GTW were observed to have a TWI score of 1 whereas almost all of the affected teeth with ETW had a BEWE score of 2. The mean D3MFT was 0.62 (95% CI 0.50, 0.73) with Decayed (D) teeth being the largest component, mean D3T was 0.36 (95% CI 0.30, 0.43). There was no significant association between socio-demographic factors and prevalence of ETW. Logistic regression analysis also showed no significant relationship between the prevalence of ETW and D3MFT (p > 0.05).

    CONCLUSIONS: Almost all adolescents examined had GTW but they were mainly early lesions. However, nearly half were found to have ETW of moderate severity (BEWE score 2). No significant relationship between the occurrence of erosive tooth wear and caries was observed in this population.

    Matched MeSH terms: Tooth Erosion/complications; Tooth Erosion/epidemiology*; Tooth Wear/complications; Tooth Wear/epidemiology*
  3. Ab-Ghani Z, Ngo H, McIntyre J
    Aust Dent J, 2007 Dec;52(4):276-81.
    PMID: 18265682
    BACKGROUND: There have been cononcerns about the dissolution of conventional glass ionomer cement (GIC) and its possible degradation when exposed to an acidic environment over time. The objective of this study was to investigate the effects of exposure of Fuji IX Fast to the simulated acidic aspects of the oral environment in terms of any change in the elemental composition of strontium (Sr), phosphorus (P), calcium (Ca) and fluorine (F) which resulted at the surface of this material.

    METHODS: Sixty-five cylindrical block of Fuji IX Fast were prepared using split moulds. The demineralizing solution was an acetate buffered demineralizing solution at pH 403. The remineralizing solution was a buffered solution containing 1.5 mM Ca, 0.9 mM P and 10 ppm F at pH 7. The blocks of Fuji IX Fast were subjected either to two-day alternating cycles of remineralization and demineralization for up to 24 days (test); 6 two-day cycles of demineralizing or remineralizing solution separately, or deionized distilled water alone (controls) or were left untreated (base line control). Mineral profiles of Ca, P, Sr and F within 100 microm of the material surface were assessed following 8, 16 and 24 days of treatment (test); 4, 8 or 12 days (controls) or for baseline control samples, using electron probe microanalysis (EPMA).

    RESULTS: There were significant changes in mineral profile in the test specimens in terms of Sr and Ca concentrations. A molecule for molecule exchange of these elements resulted between GIC and eluant solutions. Fluoride loss from the GIC occurredto the level comparable with uptake levels recorded in eluant solutions from previous studies. The ionic exchanges appeared to be the result of dissolution followed by an equilibrium-driven diffusion. These exchanges were superficial though substantial.

    CONCLUSIONS: Simulated exposure of Fuji IX to the oral environment resulted in an exchange of Ca from the bathing solutions into Fuji IX to replace any Sr which was lost to the GIC. Fluorine loss from the GIC followed previously described patterns. The possible clinical significance of this exchange was discussed.

    Matched MeSH terms: Tooth Remineralization; Tooth Demineralization
  4. Abd Rahman N, Abdullah N, Samsudin AR, Naing Mohd Ayub Sadiq L
    Malays J Med Sci, 2004 Jul;11(2):41-51.
    PMID: 22973126
    This study was done to determine the prevalence of dental anomalies and facial profile abnormality and its association with the non-syndromic cleft lip and palate (CLP) as compared to the non-cleft children. A comparative cross sectional study was conducted where the case group consist of 98 non-syndromic CLP children-unilateral (UCLP) and bilateral (BCLP) who attended the Combined Clinic at Kota Bharu Dental Clinic (KBDC) while the comparison group comprised of 109 non-cleft children who attended the outpatient clinic at KBDC. Their ages were between 3 to 12 years old. Clinical oral and facial profile examinations were carried out to look for dental anomalies (morphology, number and alignment of teeth) and facial profile abnormality. The prevalence of anomalies in morphology of teeth in CLP (24.5%) and non-cleft (10.1%), number of teeth in CLP (44.9%) and non-cleft (7.3%), mal-alignment in CLP (79.6%) and non-cleft (27.5%) and facial profile abnormality in CLP (26.5%) and non-cleft (9.1 %). There was a significant association between CLP and anomalies in morphology, number, mal-alignment and abnormality in facial profile; (p < 0.05). Therefore, there was a high prevalence and risk of dental anomalies and facial profile abnormality in the CLP children compared to the non-cleft children.
    Matched MeSH terms: Tooth
  5. Abdul Hayei NA, Yahya NA, Safii SH, Saub R, Vaithilingam RD, Baharuddin NA
    BMC Oral Health, 2021 03 31;21(1):169.
    PMID: 33789646 DOI: 10.1186/s12903-021-01540-0
    BACKGROUND: The influence of scaler tip design on root surface roughness, tooth substance loss and patients' pain perception is investigated.

    METHODS: This article was divided into the following parts: Part 1 Surface roughness and substance loss: an in vitro study, which involves intact extracted teeth sectioned and treated using a piezoelectric ultrasonic device (PM200 EMS Piezon, Switzerland) with a conventional scaler tip (FS-407) and a Perio Slim (PS) scaler tip (Perio Slim DS-016A). All sectioned samples for tooth surface roughness (n = 20) and tooth substance loss (n = 46) analyses were measured and compared using a 3D surface texture analyser and scanning electron microscope (SEM) respectively, at baseline and following scaling. Part 2 Pain Perception: a clinical study, which was a split mouth study design including 30 participants with gingivitis and/or mild chronic periodontitis; treated with supra-gingival scaling from teeth #13 to #23. Subjects were randomised to group A or group B. Group A was treated first with PS scaler tips, whereas group B was treated first with conventional scaler tips. Pain perception was recorded using the visual analogue scale (VAS).

    RESULTS: In vitro study: both scaler tips caused significant reduction in root substance roughness after scaling (p  0.05) was observed. The PS scaler tip caused statistically significantly less root substance loss (p tooth was tooth substance loss compared to a wider scaler tip design. In the clinical study, less pain was observed compared than a wide (conventional) scaler tip design.

    Matched MeSH terms: Tooth Root
  6. Abdul Rahim AH, Davies JA, Liversidge HM
    Forensic Sci Int, 2023 May;346:111654.
    PMID: 37011430 DOI: 10.1016/j.forsciint.2023.111654
    Reliability, or repeatability, of permanent tooth staging techniques is usually expressed as Cohen's Kappa. This single value obscures information about the quantity and allocation of disagreements. In this study we assess and compare intra-observer reliability of permanent tooth staging techniques described by Nolla, Moorrees et al. and Demirjian et al. The sample was panoramic radiographs of healthy dental patients made up of 100 males and 100 females aged 6-15 years. All permanent teeth on the left side (excluding third molars) were scored twice. Weighted Kappa and percentage agreement were calculated. Results show Kappa values for all teeth combined as 0.918, 0.922 and 0.938 for Demirjian (number of teeth N = 2682), Nolla (N = 2698) and Moorrees (N = 2674) respectively. A comparison of Kappa values between upper and lower teeth showed marginally higher values for upper incisors and lower molar for all three scoring methods. Small differences in Kappa values were noted between tooth types with the upper first molar having smaller values than other teeth. Percentage agreement ranged from 81 % (Moorrees), 86 % (Nolla) to 87 % (Demirjian). Tooth stage differences between first and second assessments were not more than one stage. Our findings show that Demirjian scoring is marginally more reliable than Nolla or Moorrees. We suggest that (1) data for reliability are tabulated in full to show the quantity and allocation of disagreement between first and second readings, and (2) that the reliability sample is sufficiently large with a wide age range to include multiple different tooth stages.
    Matched MeSH terms: Tooth*
  7. Abdul Razak I, Jaafar N, Mat Nor G
    J Ir Dent Assoc, 1989;35(1):39-41.
    PMID: 2634714
    The reasons underlying the need for extraction of 2765 permanent teeth carried out over a 6 month period in the Out-patient Clinic in the Dental Faculty, University of Malaya, was investigated. The present study showed that dental caries (67.4%) is the leading cause for extraction followed by periodontal disease (19.6%). Caries accounted for the highest proportion of extractions up to the age of 50 whereas periodontal disease becomes the major factor beyond this age. The highest frequency of extractions carried out was between the ages of 21-30.
    Matched MeSH terms: Tooth Extraction/statistics & numerical data
  8. Abdul Razak, A.A.
    Ann Dent, 1998;5(1):-.
    MyJurnal
    Much interest has been generated in tooth-co loured inlays/onlays especially with patient's concern for esthetic appearance and the dentist's appreciation for additional strength of the restored tooth, together with bonding. Many types of tooth-coloured inlay/onlay restorations are available but none have undergone extensive and long term research. The different types of inlay/onlay restorations together with steps in their preparation are presented in this paper.
    Matched MeSH terms: Tooth
  9. Abdul Satar H, Yaakub A, Md Shukri N, Ahmad Tajudin LS
    Cureus, 2021 Apr 09;13(4):e14392.
    PMID: 33981511 DOI: 10.7759/cureus.14392
    Paediatric orbital cellulitis is a potential sight- and life-threatening condition. It is a serious infection in children that can result in significant complications, including blindness, cavernous sinus thrombosis, cerebral venous sinus thrombosis, meningitis, subdural empyema, and brain abscess. Of the patients with orbital cellulitis, 17% died from meningitis, and 20% of the survivors had permanent loss of vision. Therefore, the potential for sight- and life-threatening complications makes prompt diagnosis and early treatment very important. We report here a case of a two-year-old girl who presented with a three-day history of left periorbital swelling, preceded by left upper perioral swelling that extended upward to the left cheek and left lower lid and was associated with low-grade fever. The patient had been admitted and was treated as having left preseptal with facial cellulitis; the patient was started on intravenous amoxicillin/clavulanic acid (200 mg three times per day dose), and chloramphenicol ointment was applied to the periorbital area. On day 3, the condition worsened, and dental examination showed multiple dental caries, upper gum swelling and abscess, and mobility of teeth 61 and 62 (two baby teeth). Contrast-enhanced computed tomography (CECT) of the orbit, paranasal, and brain showed a left periosteal abscess collection extending to the inferomedial region of the orbit. Examination and tooth extraction were performed under general anesthesia. The intraoperative results showed the presence of a left upper gum abscess, which was possibly the primary source of infection. Clinical improvement was observed postoperatively. Orbital cellulitis can be a complication of a dental abscess. This case emphasizes the importance of primary tooth care in children. A lack of care can result in fatal complications.
    Matched MeSH terms: Tooth Extraction; Tooth, Deciduous
  10. Abdul Wahab RM, Zainal Ariffin SH, Yeen WW, Ahmad NA, Senafi S
    ScientificWorldJournal, 2012;2012:236427.
    PMID: 22629122 DOI: 10.1100/2012/236427
    Three specific orthodontic tooth movement genes, that is, FCRL1, HSPG2, and LAMB2 were detected at upper first premolar (with appliance) dental pulp tissue by using GeneFishing technique as compared to lower first premolar (without appliance). These three differentially expressed genes have the potential as molecular markers during orthodontic tooth movement by looking at molecular changes of pulp tissue.
    Matched MeSH terms: Tooth Movement*
  11. Abdulla AM, Sivadas G, Surej Kumar LK, Sheejith Hari Peeceeyen CS, Vedam V
    Case Rep Med, 2017;2017:9483738.
    PMID: 28883834 DOI: 10.1155/2017/9483738
    Ameloblastic fibroodontoma is a benign mixed odontogenic neoplasm considered in patients with asymptomatic swelling and unerupted teeth that exhibit histologic features between ameloblastic fibroma and complex odontoma. Radiographically, this lesion appears as radiolucency admixed with focal radio opaque masses of irregular shapes and sizes. This lesion is confirmed by the presence of proliferating odontogenic epithelium, ectomesenchyme, and dental hard tissue formation on pathological analysis supplementing clinical and radiographic findings. As this tumour is less commonly seen in routine clinical practice, ameloblastic fibroodontoma with detailed orofacial features and periodic approach to its diagnosis is discussed. This paper reports a case of ameloblastic fibroodontoma of the mandible in a 6-year-old male patient with an uncommon case presentation and review of the literature.
    Matched MeSH terms: Tooth, Unerupted
  12. Abdullah D, Liew AK, Wan Noorina WA, Khoo S, Wee FC
    Dent Traumatol, 2015 Oct;31(5):403-8.
    PMID: 26058666 DOI: 10.1111/edt.12191
    OBJECTIVES: To assess and compare the knowledge of rugby players regarding first-aid measures for dental injuries.

    METHODS: A cross-sectional study was conducted at rugby tournaments in 2009 and 2010 on players aged 16 and over. Convenient sampling was performed. A total of 456 self-administered questionnaires were returned. Data collected were analysed using SPSS 21. Descriptive analysis was undertaken for the demographic data. The subjects were classified according to their experience of sustaining each type of injury. Cross-tabulation and chi-square tests were carried out to compare the responses. When the expected cell count was less than five, Fisher's exact test was used. The level of significance was set at P < 0.05.

    RESULTS: The prevalence of self-reported dental injuries was as follows: tooth fracture (19.3%), luxation (6.6%) and avulsion (1.1%). Significant differences were found, whereby 52.2% of those who had no history of tooth fracture were more likely to seek immediate treatment (P < 0.001), whereas 42% of those who previously experienced tooth fracture claimed that they would only visit a dentist if they experienced pain (P = 0.001). Management of luxation and avulsion did not differ significantly between the groups. However, about half of those who did not have a history of tooth avulsion admitted to not knowing the correct answer, while three of five casualties would keep the tooth iced.

    CONCLUSIONS: Knowledge of the management of tooth fracture and storage medium differs between previous casualties and non-casualties. Overall, knowledge of dental trauma management was insufficient, suggesting the need to educate and train the players.

    Matched MeSH terms: Tooth Injuries/epidemiology; Tooth Injuries/therapy*
  13. Abdullah D, Soo SY, Kanagasingam S
    Singapore Dent J, 2016 Dec;37:21-26.
    PMID: 27916252 DOI: 10.1016/j.sdj.2016.01.001
    BACKGROUND: Dental and maxillofacial injuries are one of the areas of concern highlighted in the Malaysian National Oral Health Plan 2011-2020. General dental practitioners (GDPs) have the responsibility of diagnosing and assessing dental trauma and determining the prognosis and outcomes of trauma along with its management. The purpose of this study was to evaluate the knowledge base and preferred methods of general dental practitioners regarding the management of avulsed tooth.

    METHODS: A random convenient sampling methodology was employed for sample selection. A pre-tested 11-item questionnaire was validated on the dental officers. The survey was distributed to 182 GDPs attending the annual Malaysian Dental Association conference in January 2010. The data obtained was statistically analyzed using descriptive analysis and logistic regression was employed to predict the probability of achieving high scores.

    RESULTS: A total of 182 general dental practitioners participated in the study, with the majority being female (n=153, 75%). The place of practice significantly affected the knowledge score. In the group that scored more than 80 points (n=84, 46%), 76% of them worked with government hospitals. Age, work duration and number of traumatised teeth previously treated had no significant effect. The odds ratio for place of practice indicates that respondents who work in government hospitals are 3.6 times more likely to score more than 80 points compared to those who worked in private clinics (OR=3.615, P=0.001).

    CONCLUSION: The knowledge level on the management of avulsed tooth among general dental practitioners in Malaysia needs to be improved. Strategies in improvement of the Malaysian dental educational system, continuous dental educational activities and utilisation of guidelines on trauma management should be recommended to increase the knowledge level of avulsed tooth management to ensure good treatment outcomes.

    CLINICAL IMPLICATION: Trauma prevention and further education regarding the management of avulsed tooth is an essential requirement to improve general dental practitioners knowledge and clinical skills.

    Matched MeSH terms: Tooth Avulsion
  14. Abdullah D, Kanagasingam S, Luke D
    Sains Malaysiana, 2013;42:81-84.
    The aim of the study was to determine the frequency, size and location of apical and lateral foramina on anterior teeth. A total of 100 anterior teeth consisting of maxillary and mandibular incisors and canines were fixed in 10% formalin. Periodontal tissue remnants were mechanically removed and teeth were stained in 2% aqueous silver nitrate. The teeth were dried and examined using a Leica MZ 7.5 zoom stereomicroscope. The size of apical and lateral foramina and their distance from the anatomical apex of the tooth were measured directly using a calibrated eyepiece scale. Accessory foramina more than 1.8 mm from the apex were regarded as lateral foramina. Eighteen percent of teeth possessed more than one apical foramen. Seven teeth (three maxillary centrals, three maxillary canines, one mandibular lateral) had 11 lateral foramina each. The mean diameter of the lateral foramina was 0.14 mm (SD = 0.08) and their mean distance from the apex was 4.49 mm (SD = 2.63, range 1.9-10.5 mm). Multiple foramina were most common on maxillary canines and least common on maxillary laterals. The mean diameter of apical foramina for all teeth possessing a single foramen was 0.35 mm (SD = 0.10) and the mean apical foramen diameter for all teeth with multiple apical foramina was 0.22 mm (SD = 0.08). Most anterior teeth possess one apical foramen and no lateral foramina; about 20% possessed more than one apical foramen and about 10% possessed one or more lateral foramina. These findings should be considered when root-treating anterior teeth.
    Matched MeSH terms: Tooth Root; Tooth Apex
  15. Abdullah, M., Che Ab Aziz, Z.A., Roslan Saub, Murat, N., Sulaiman, E., Hashim, N., et al.
    Ann Dent, 2009;16(1):9-14.
    MyJurnal
    The objectives of this study were to assess the practice of molar root canal treatment (RCT) among general dental practitioners (GDPs), confidence level of GDPs in performing molar RCT and to identify factors that influence their confidence in managing molar RCI. One hundred questionnaires were distributed to GDPs in Kuala Lumpur and Selangor. Fifty questionnaires were delivered by mail and the remaining fifty were hand-delivered. All data was coded and verified. Fiftysix OOPs rcsponded to the questionnaires. Only fortyfive questionnaires were accepted (n=45) as eleven OOPs had never performed molar RCI. Most of the OOPs followed the standard quality guidelines while performing molar RCT except for vitality testing (36.4%) and rubber dam usage (27.3%). In this study, 100% of the GDPs were confident in making diagnosis of perinidicular diseases and 95.4% were confident in performing molar RCT from history taking until obturation. Patients' tolerance and availability of instruments and materials (97.7%) were two factors that most influence the GDPs' confidence level. The. least influcncing factor was undergraduate training (78.1 %). Attending seminars and lectures on endodontic, large number of molar teeth treated for ReT, patLents' affordability to pay for RCT and postgraduate training also influencc GDPs' confidence to a certain degree. From this study, it can be concluded that majority of OOPs complied standard quality guidelines except for vitality testing and rubber dam usage. Most of them were confident in performing molar RCT and were greatly influenced by the availability or"instruments and materials in their clinic and patients' tolerance in receiving molar RCI.
    Matched MeSH terms: Tooth Root
  16. Abu Bakar N, Lee CL
    Med J Malaysia, 2013;68(1):73-5.
    PMID: 23466774
    Osteo-odontokeratoprosthesis (OOKP) surgery is a technique used to replace damaged cornea in blind patients for whom cadaveric transplantation is not feasible. OOKP surgery is a complex procedure requiring lifetime follow-up. The preservation of the osteo-odontolamina is the vital feature in maintaining the stability of the OOKP. Early detection of lamina resorption enables early prophylactic measures to be taken and prevent resorption-related complications. This case illustrates the radiological findings of the first OOKP surgery in Malaysia and the role of multidetector computed tomography (MDCT) in postoperative management of OOKP surgery.
    Matched MeSH terms: Tooth Root
  17. Abu Bakar A, Mohd Nor NA, Ab-Murat N, Jaafar N
    Int J Dent Hyg, 2015 Aug;13(3):199-205.
    PMID: 25040653 DOI: 10.1111/idh.12095
    OBJECTIVE: To assess Malaysian dental therapists' perceptions of their job satisfaction and future roles.
    METHODS: A nationwide postal survey involving all Malaysian dental therapists who met the inclusion criteria (n = 1726).
    RESULTS: The response rate was 76.8%. All respondents were females; mean age 35.4 years (SD = 8.4). Majority were married (85.5%) and more than one-half had a working experience of <10 years (56.1%). Majority worked in community dental service (94.3%) and in urban areas (61.7%). Overall, they were highly satisfied with most aspects of their career. However, they were least satisfied with administrative workload (58.1%), career advancement opportunities (51.9%) and remuneration package; specifically income (45.2%), allowances (45.2%) and non-commensurate between pay and performance (44.0%). Majority perceived their role as very important in routine clinical tasks such as examination and diagnosis, preventive treatment, extraction of deciduous teeth and oral health promotion. However, fewer than one-half consider complex treatment such as placement of preformed crowns on deciduous teeth (37.1%) and extraction of permanent teeth (37.2%) as very important tasks.
    CONCLUSION: Majority expressed high career satisfaction with most aspects of their employment but expressed low satisfaction in remuneration, lack of career advancement opportunities and administrative tasks. We conclude that most Malaysian dental therapists have positive perceptions of their current roles but do not favour wider expansion of their roles. These findings imply that there was a need to develop a more attractive career pathway for therapists to ensure sustainability of effective primary oral healthcare delivery system for Malaysia's children.
    Matched MeSH terms: Tooth, Deciduous
  18. Adam Husein, Huwaina Abd. Ghani, Fazal Reza
    MyJurnal
    Replacing a single missing anterior tooth can be a challenge. Many factors need to be considered when choosing the appropriate treatment. Several treatment options are well established. This case report is to present the use of a cast cobalt chrome partial denture with custom made porcelain tooth to improve aesthetic. The overall shade of the artificial tooth were nicely matched with the adjacent teeth and definite enamel translucency could be achieved which would not be possible with acrylic or even with readymade porcelain tooth.
    Matched MeSH terms: Tooth, Artificial
  19. Afishah Alias, Siti Rahayu Mohd Hashim, Wajir, Julynnie, Fauziah Abdul Aziz, Mihaly, Judith
    MyJurnal
    Unaffected, affected and heavily affected teeth enamel were studied by using FT-Raman spectroscopy. The 14 permanent teeth’s enamel surface were measured randomly, resulting in total n=43 FT-Raman spectra. The results obtained from FT-Raman spectra of heavily affected, affected and unaffected tooth’s enamel surfaces did not show any significant difference. In this study, Kruskal-Wallis and Wilcoxon rank sum tests were used to compare the intensity between the categories of enamel as well as the surfaces of teeth samples.
    Matched MeSH terms: Tooth
  20. Ahmad Azlina, Berahim Zurairah, Sidek Mohamad Ros, Mokhtar Khairani Idah, Samsudin Abdul Rani
    MyJurnal
    Mitochondrial DNA (mtDNA) is a hereditary material located in mitochondria and is normally maternally inherited. Mutational analysis performed on mtDNA proved that the mutations are closely related with a number of genetic illnesses, besides being exploitable for forensic identification. Those findings imply the importance of mtDNA in the scientific field. MtDNA can be found in abundance in tooth dentin where it is kept protected by the enamel, the hardest outer part of the tooth. In this study, two techniques of mtDNA extraction were compared to determine the efficacy between the two techniques. Teeth used for the study was collected from Dental Clinic, Hospital Universiti Sains Malaysia. After the removal of tooth from the tooth socket of the patient, the tooth was kept at -20C until use. Later, pulp tissue and enamel was excised using dental bur and only the root dentin was utilized for the isolation of mtDNA by crushing it mechanically into powdered form. MtDNA was extracted using the two published methods, Pfeifer and Budowle and then subjected to spectrophotometry DNA quantification and purity, Polymerase chain reaction (PCR) amplification of hypervariable-two region of mtDNA, followed by DNA sequencing to analyze the reliability of the extraction techniques. In conclusion, both techniques proved to be efficient and capable for the extraction of mtDNA from tooth dentin.
    Matched MeSH terms: Tooth; Tooth Socket
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