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Fulltext 'Movers and shakers' in the regulation of fruit ripening: a cross-dissection of climacteric versus non-climacteric fruit

Cherian S, Figueroa CR, Nair H

J Exp Bot, 2014 Sep;65(17):4705-22.
PMID: 24994760 DOI: 10.1093/jxb/eru280

Fruit ripening is a complex and highly coordinated developmental process involving the expression of many ripening-related genes under the control of a network of signalling pathways. The hormonal control of climacteric fruit ripening, especially ethylene perception and signalling transduction in tomato has been well characterized. Additionally, great strides have been made in understanding some of the major regulatory switches (transcription factors such as RIPENING-INHIBITOR and other transcriptional regulators such as COLOURLESS NON-RIPENING, TOMATO AGAMOUS-LIKE1 and ETHYLENE RESPONSE FACTORs), that are involved in tomato fruit ripening. In contrast, the regulatory network related to non-climacteric fruit ripening remains poorly understood. However, some of the most recent breakthrough research data have provided several lines of evidences for abscisic acid- and sucrose-mediated ripening of strawberry, a non-climacteric fruit model. In this review, we discuss the most recent research findings concerning the hormonal regulation of fleshy fruit ripening and their cross-talk and the future challenges taking tomato as a climacteric fruit model and strawberry as a non-climacteric fruit model. We also highlight the possible contribution of epigenetic changes including the role of plant microRNAs, which is opening new avenues and great possibilities in the fields of fruit-ripening research and postharvest biology.

Matched MeSH terms: Fruit/growth & development; Lycopersicon esculentum/growth & development; Fragaria/growth & development
'Targeting the feast of a sleeping beast': Nutrient and mineral dependencies of encysted Acanthamoeba castellanii

Baig AM, Khan NA, Katyara P, Lalani S, Baig R, Nadeem M, et al.

Chem Biol Drug Des, 2021 01;97(1):18-27.
PMID: 32602961 DOI: 10.1111/cbdd.13755

Acanthamoeba spp. cause a corneal infection, Acanthamoeba keratitis (AK), and a cerebral infection, granulomatous amoebic encephalitis (GAE). Though aggressive chemotherapy has been able to kill the active trophozoite form of Acanthamoeba, the encysted form of this parasite has remained problematic to resist physiological concentrations of drugs. The emergence of encysted amoeba into active trophozoite form poses a challenge to eradicate this parasite. Acanthamoeba trophozoites have active metabolic machinery that furnishes energy in the form of ATPs by subjecting carbohydrates and lipids to undergo pathways including glycolysis and beta-oxidation of free fatty acids, respectively. However, very little is known about the metabolic preferences and dependencies of an encysted trophozoite on minerals or potential nutrients that it consumes to live in an encysted state. Here, we investigate the metabolic and nutrient preferences of the encysted trophozoite of Acanthamoeba castellanii and the possibility to target them by drugs that act on calcium ion dependencies of the encysted amoeba. The experimental assays, immunostaining coupled with bioinformatics tools show that the encysted Acanthamoeba uses diverse nutrient pathways to obtain energy in the quiescent encysted state. These findings highlight potential pathways that can be targeted in eradicating amoebae cysts successfully.

Matched MeSH terms: Acanthamoeba castellanii/growth & development
2-dodecanol (decyl methyl carbinol) inhibits hyphal formation and SIR2 expression in C. albicans

Lim CS, Wong WF, Rosli R, Ng KP, Seow HF, Chong PP

J Basic Microbiol, 2009 Dec;49(6):579-83.
PMID: 19810039 DOI: 10.1002/jobm.200900035

Candida albicans is capable of undergoing yeast-hypha transition to attain pathogenicity in humans. In this study, we investigated the differential expression of CaSIR2 via quantitative real-time PCR (qPCR), during yeast-hypha transition with and without the presence of 2-dodecanol. SIR2 transcript levels were found to be significantly enhanced after hyphal induction as compared to the yeast form. This study found that 2-dodecanol is able to inhibit hyphal development and block SIR2 up-regulation, even in hyphal-inducing growth conditions. We suggest that SIR2 may be involved in Candida albicans quorum-sensing and serum-induced yeast-hyphae transition via the Ras1-cAMP-Efg1 signalling cascade.

Matched MeSH terms: Candida albicans/growth & development; Hyphae/growth & development*
Fulltext 5α-Reduced neurosteroids sex-dependently reverse central prenatal programming of neuroendocrine stress responses in rats

Brunton PJ, Donadio MV, Yao ST, Greenwood M, Seckl JR, Murphy D, et al.

J Neurosci, 2015 Jan 14;35(2):666-77.
PMID: 25589761 DOI: 10.1523/JNEUROSCI.5104-13.2015

Maternal social stress during late pregnancy programs hypothalamo-pituitary-adrenal (HPA) axis hyper-responsiveness to stressors, such that adult prenatally stressed (PNS) offspring display exaggerated HPA axis responses to a physical stressor (systemic interleukin-1β; IL-1β) in adulthood, compared with controls. IL-1β acts via a noradrenergic relay from the nucleus tractus solitarii (NTS) to corticotropin releasing hormone neurons in the paraventricular nucleus (PVN). Neurosteroids can reduce HPA axis responses, so allopregnanolone and 3β-androstanediol (3β-diol; 5α-reduced metabolites of progesterone and testosterone, respectively) were given subacutely (over 24 h) to PNS rats to seek reversal of the "programmed" hyper-responsive HPA phenotype. Allopregnanolone attenuated ACTH responses to IL-1β (500 ng/kg, i.v.) in PNS females, but not in PNS males. However, 3β-diol normalized HPA axis responses to IL-1β in PNS males. Impaired testosterone and progesterone metabolism or increased secretion in PNS rats was indicated by greater plasma testosterone and progesterone concentrations in male and female PNS rats, respectively. Deficits in central neurosteroid production were indicated by reduced 5α-reductase mRNA levels in both male and female PNS offspring in the NTS, and in the PVN in males. In PNS females, adenovirus-mediated gene transfer was used to upregulate expression of 5α-reductase and 3α-hydroxysteroid dehydrogenase mRNAs in the NTS, and this normalized hyperactive HPA axis responses to IL-1β. Thus, downregulation of neurosteroid production in the brain may underlie HPA axis hyper-responsiveness in prenatally programmed offspring, and administration of 5α-reduced steroids acutely to PNS rats overrides programming of hyperactive HPA axis responses to immune challenge in a sex-dependent manner.

Matched MeSH terms: Hypothalamo-Hypophyseal System/growth & development; Paraventricular Hypothalamic Nucleus/growth & development; Pituitary-Adrenal System/growth & development; Solitary Nucleus/growth & development
A New Species of Simulium (Asiosimulium) (Diptera: Simuliidae) from Thailand

Takaoka H, Srisuka W, Saeung A

J Med Entomol, 2018 05 04;55(3):569-574.
PMID: 29361148 DOI: 10.1093/jme/tjx242

Simulium (Asiosimulium) saeungae sp. nov. (Diptera: Simuliidae) is described based on females, males, pupae, and mature larvae collected from Nan Province, Northern Thailand. It is characterized by the medium-long cerci in the female, enlarged hind basitarsus, and broad ventral plate with its posterior margin not deeply concave in the male, arborescent pupal gill with 42-56 filaments in the pupa and smaller number of primary rays of the labral fan (30-33) in the larva. This is the fifth species of the subgenus Asiosimulium, the second smallest among 10 subgenera in the Oriental Region. Taxonomic notes are given to distinguish this new species from the three known species from Thailand and one from Nepal.

Matched MeSH terms: Larva/growth & development; Pupa/growth & development; Simuliidae/growth & development
A New Species of Simulium (Gomphostilbia) (Diptera: Simuliidae) From Kalimantan, Indonesia, With Keys to Identify 19 Bornean Species of the Subgenus Gomphostilbia

Takaoka H, Sofian-Azirun M, Ya'cob Z, Chen CD, Low VL, Harmonis

J Med Entomol, 2016 07;53(4):798-806.
PMID: 27099400

A new simuliid species, Simulium kalimantanense sp. nov., is described on the basis of females, males, pupae, and mature larvae from East Kalimantan, Indonesia, and is assigned to the Simulium banauense species-group of Simulium (Gomphostilbia). This new species has close similarities to S alienigenum Takaoka from the Philippines, in many characters including the adult antennal color pattern and pupal gill with four long filaments arranged in two pairs each bearing a long stalk, but is distinguished from the latter in the female by the longer sensory vesicle and in the pupa by the gill with an elongate common basal stalk. Simulium kalimantanense sp. nov. is the first member of the S. banauense group in Borneo, and marks the most southerly distribution of the group. Keys to identify 19 Bornean species of the subgenus Gomphostilbia are provided.

Matched MeSH terms: Larva/growth & development; Pupa/growth & development; Simuliidae/growth & development
A New Species of the Simulium (Simulium) argentipes Species-Group (Diptera: Simuliidae) From Taiwan, and Its Phylogenetic Relationships With Four Related Species

Takaoka H, Low VL, Tan TK, Huang YT, Fukuda M, Ya'cob Z

J Med Entomol, 2018 06 28;55(4):884-892.
PMID: 29538704 DOI: 10.1093/jme/tjy028

A new black fly species, Simulium haiduanense Takaoka, Low & Huang (Diptera: Simuliidae), is described on the basis of females, males, pupae, and mature larvae from Taiwan. This new species is placed in the Simulium argentipes species-group of the subgenus Simulium (Diptera: Simuliidae) and is characterized by the yellowish female legs, ovipositor valves rounded apically and with its inner margin concave, claw with a small subbasal tooth, male style without a basal protuberance, pupal gill with eight filaments, corbicular cocoon, and larval abdomen lacking paired protuberances. It represents the first record of the S. argentipes species-group from Taiwan. Taxonomic notes are given to separate this new species from all eight species in the same species-group. The phylogenetic relationships of this new species with four related species are presented.

Matched MeSH terms: Larva/growth & development; Pupa/growth & development; Simuliidae/growth & development
Fulltext A P. falciparum NF54 Reporter Line Expressing mCherry-Luciferase in Gametocytes, Sporozoites, and Liver-Stages

Marin-Mogollon C, Salman AM, Koolen KMJ, Bolscher JM, van Pul FJA, Miyazaki S, et al.

Front Cell Infect Microbiol, 2019;9:96.
PMID: 31058097 DOI: 10.3389/fcimb.2019.00096

Transgenic malaria parasites expressing fluorescent and bioluminescent proteins are valuable tools to interrogate malaria-parasite biology and to evaluate drugs and vaccines. Using CRISPR/Cas9 methodology a transgenic Plasmodium falciparum (Pf) NF54 line was generated that expresses a fusion of mCherry and luciferase genes under the control of the Pf etramp10.3 gene promoter (line mCherry-luc@etramp10.3). Pf etramp10.3 is related to rodent Plasmodium uis4 and the uis4 promoter has been used to drive high transgene expression in rodent parasite sporozoites and liver-stages. We examined transgene expression throughout the complete life cycle and compared this expression to transgenic lines expressing mCherry-luciferase and GFP-luciferase under control of the constitutive gapdh and eef1a promoters. The mCherry-luc@etramp10.3 parasites express mCherry in gametocytes, sporozoites, and liver-stages. While no mCherry signal was detected in asexual blood-stage parasites above background levels, luciferase expression was detected in asexual blood-stages, as well as in gametocytes, sporozoites and liver-stages, with the highest levels of reporter expression detected in stage III-V gametocytes and in sporozoites. The expression of mCherry and luciferase in gametocytes and sporozoites makes this transgenic parasite line suitable to use in in vitro assays that examine the effect of transmission blocking inhibitors and to analyse gametocyte and sporozoite biology.

Matched MeSH terms: Plasmodium falciparum/growth & development*; Sporozoites/growth & development
A Volume Celebrating the Life of Don Colless, Australian Dipterist 1922–2012

Yeates DK, Cranston PS

Zootaxa, 2013;3680:1-211.
PMID: 26146692 DOI: 10.11646/zootaxa.3680.1.1

Donald Henry Colless (24 August 1922–16 February 2012) was a taxonomist at the Australian National Insect Collection (ANIC) from 1960 until his retirement in 1987. He continued working in ANIC as an Honorary Fellow until his death in 2012. Don’s main scientific interests were in the taxonomy and biology of true flies, and in the theory of phylogenetic reconstruction and classification. Don was trained in entomology at the University of Sydney, and spent nearly two decades of his early career in Asia studying mosquitoes and disease transmission, first in the Army during the Second World War in New Guinea and Borneo (1942–45), then after the war in North Borneo (1947–1952) and as a lecturer in the Department of Parasitology at the University of Malaya (1952–1960) in Singapore. We list the 127 scientific papers and book chapters that Don published during his scientific career that spanned 64 years. Six of these papers were published in the prestigious international journal Nature, and he was Chief Curator of the ANIC from 1971–1977. Don had extremely broad taxonomic interests, publishing on the taxonomy of 18 families of Diptera that spanned the phylogenetic breadth of the order. He described as new to science the fly families Perissommatidae and Axiniidae, thirteen new genera and over 120 species and, with David McAlpine, authored the Diptera chapters in both editions of The Insects of Australia (Melbourne University Press, 1970 and 1991). He published a number of influential critiques of cladistic theory in the 1960's and 1970's, and advocated a phenetic approach to the discovery of taxonomic groups, and phylogenetic reconstruction.

Matched MeSH terms: Animal Structures/growth & development; Diptera/growth & development
A brief period of darkness induces changes in fatty acid biosynthesis towards accumulation of saturated fatty acids in Chlorella vulgaris UMT-M1 at stationary growth phase

Cha TS, Yee W, Phua PSP, Loh SH, Aziz A

Biotechnol Lett, 2021 Apr;43(4):803-812.
PMID: 33438120 DOI: 10.1007/s10529-021-03077-2

OBJECTIVE: The effects of a brief (3 days) and prolonged (6 days) period of incubation in darkness and light on the biomass content, lipid content and fatty acid profile in Chlorella vulgaris UMT-M1 were determined.
RESULTS: Three days of incubation in darkness increased saturated fatty acid (SFA) content from 34.0 to 41.4% but decreased monounsaturated fatty acid (MUFA) content from 36.7 to 29.8%. Palmitic acid (C16:0) content was increased from 23.2 to 28.9%, whereas oleic acid (C18:1) content was reduced from 35.4 to 28.8%. Total oil content was slightly decreased from 20.4 to 18.7% after 3 days of darkness, without a significant reduction in biomass compared to 3 days of incubation in light. Biomass and oil content was highest in cultures incubated for 6 days in light, however the stimulatory and inhibitory effects of darkness (or light) on SFA and MUFA content was no longer present at 6 days of incubation.
CONCLUSIONS: Findings from this study suggests that fatty acid composition in C. vulgaris could be modulated to favor either C16:0 or C18:1 by a brief period of either darkness or light incubation, prior to harvesting.

Matched MeSH terms: Chlorella vulgaris/growth & development*
A case of Hunterellus hookeri parasitization of ticks in Petaling Jaya, Peninsula Malaysia

Cheong WH, Rajamanikam C, Mahadevan S

Southeast Asian J. Trop. Med. Public Health, 1978 Sep;9(3):456-8.
PMID: 749234
Matched MeSH terms: Hymenoptera/growth & development*; Wasps/growth & development*
Fulltext A case of human enteric myiasis due to larvae of Hermetia illucens (Family: Stratiomyiadae): first report in Malaysia

Lee HL, Chandrawathani P, Wong WY, Tharam S, Lim WY

Malays J Pathol, 1995 Dec;17(2):109-11.
PMID: 8935136

A case of true enteric myiasis in a 7-year-old girl is reported. Two larvae were obtained from the vomitus of the patient. After processing and identification, the larvae were found to be those of Hermetia illucens (Soldier Fly). This is the first case of true enteric myiasis due to these larvae in Malaysia.

Matched MeSH terms: Diptera/growth & development*
A climate distribution model of malaria transmission in Sudan

Musa MI, Shohaimi S, Hashim NR, Krishnarajah I

Geospat Health, 2012 Nov;7(1):27-36.
PMID: 23242678

Malaria remains a major health problem in Sudan. With a population exceeding 39 million, there are around 7.5 million cases and 35,000 deaths every year. The predicted distribution of malaria derived from climate factors such as maximum and minimum temperatures, rainfall and relative humidity was compared with the actual number of malaria cases in Sudan for the period 2004 to 2010. The predictive calculations were done by fuzzy logic suitability (FLS) applied to the numerical distribution of malaria transmission based on the life cycle characteristics of the Anopheles mosquito accounting for the impact of climate factors on malaria transmission. This information is visualized as a series of maps (presented in video format) using a geographical information systems (GIS) approach. The climate factors were found to be suitable for malaria transmission in the period of May to October, whereas the actual case rates of malaria were high from June to November indicating a positive correlation. While comparisons between the prediction model for June and the case rate model for July did not show a high degree of association (18%), the results later in the year were better, reaching the highest level (55%) for October prediction and November case rate.

Matched MeSH terms: Anopheles/growth & development
Fulltext A clinical tool to predict Plasmodium vivax recurrence in Malaysia

Mat Ariffin N, Islahudin F, Kumolosasi E, Makmor-Bakry M

BMC Infect Dis, 2017 12 08;17(1):759.
PMID: 29216842 DOI: 10.1186/s12879-017-2868-9

BACKGROUND: Recurrence rates of Plasmodium vivax infections differ across various geographic regions. Interestingly, South-East Asia and the Asia-Pacific region are documented to exhibit the most frequent recurrence incidences. Identifying patients at a higher risk for recurrences gives valuable information in strengthening the efforts to control P. vivax infections. The aim of the study was to develop a tool to identify P. vivax- infected patients that are at a higher risk of recurrence in Malaysia.
METHODS: Patient data was obtained retrospectively through the Ministry of Health, Malaysia, from 2011 to 2016. Patients with incomplete data were excluded. A total of 2044 clinical P. vivax malaria cases treated with primaquine were included. Data collected were patient, disease, and treatment characteristics. Two-thirds of the cases (n = 1362) were used to develop a clinical risk score, while the remaining third (n = 682) was used for validation.
RESULTS: Using multivariate analysis, age (p = 0.03), gametocyte sexual count (p = 0.04), indigenous transmission (p = 0.04), type of treatment (p = 0.12), and incomplete primaquine treatment (p = 0.14) were found to be predictors of recurrence after controlling for other confounding factors; these predictors were then used in developing the final model. The beta-coefficient values were used to develop a clinical scoring tool to predict possible recurrence. The total scores ranged between 0 and 8. A higher score indicated a higher risk for recurrence (odds ratio [OR]: 1.971; 95% confidence interval [CI]: 1.562-2.487; p ≤ 0.001). The area under the receiver operating characteristic (ROC) curve of the developed (n = 1362) and validated model (n = 682) was of good accuracy (ROC: 0.728, 95% CI: 0.670-0.785, p value

Matched MeSH terms: Plasmodium vivax/growth & development
A comparative genomic analysis of the alkalitolerant soil bacterium Bacillus lehensis G1

Noor YM, Samsulrizal NH, Jema'on NA, Low KO, Ramli AN, Alias NI, et al.

Gene, 2014 Jul 25;545(2):253-61.
PMID: 24811681 DOI: 10.1016/j.gene.2014.05.012

Bacillus lehensis G1 is a Gram-positive, moderately alkalitolerant bacterium isolated from soil samples. B. lehensis produces cyclodextrin glucanotransferase (CGTase), an enzyme that has enabled the extensive use of cyclodextrin in foodstuffs, chemicals, and pharmaceuticals. The genome sequence of B. lehensis G1 consists of a single circular 3.99 Mb chromosome containing 4017 protein-coding sequences (CDSs), of which 2818 (70.15%) have assigned biological roles, 936 (23.30%) have conserved domains with unknown functions, and 263 (6.55%) have no match with any protein database. Bacillus clausii KSM-K16 was established as the closest relative to B. lehensis G1 based on gene content similarity and 16S rRNA phylogenetic analysis. A total of 2820 proteins from B. lehensis G1 were found to have orthologues in B. clausii, including sodium-proton antiporters, transport proteins, and proteins involved in ATP synthesis. A comparative analysis of these proteins and those in B. clausii and other alkaliphilic Bacillus species was carried out to investigate their contributions towards the alkalitolerance of the microorganism. The similarities and differences in alkalitolerance-related genes among alkalitolerant/alkaliphilic Bacillus species highlight the complex mechanism of pH homeostasis. The B. lehensis G1 genome was also mined for proteins and enzymes with potential viability for industrial and commercial purposes.

Matched MeSH terms: Bacillus/growth & development
Fulltext A comparison of the clinical, laboratory and epidemiological features of two divergent subpopulations of Plasmodium knowlesi

Hu TH, Rosli N, Mohamad DSA, Kadir KA, Ching ZH, Chai YH, et al.

Sci Rep, 2021 10 11;11(1):20117.
PMID: 34635723 DOI: 10.1038/s41598-021-99644-8

Plasmodium knowlesi, a simian malaria parasite responsible for all recent indigenous cases of malaria in Malaysia, infects humans throughout Southeast Asia. There are two genetically distinct subpopulations of Plasmodium knowlesi in Malaysian Borneo, one associated with long-tailed macaques (termed cluster 1) and the other with pig-tailed macaques (cluster 2). A prospective study was conducted to determine whether there were any between-subpopulation differences in clinical and laboratory features, as well as in epidemiological characteristics. Over 2 years, 420 adults admitted to Kapit Hospital, Malaysian Borneo with knowlesi malaria were studied. Infections with each subpopulation resulted in mostly uncomplicated malaria. Severe disease was observed in 35/298 (11.7%) of single cluster 1 and 8/115 (7.0%) of single cluster 2 infections (p = 0.208). There was no clinically significant difference in outcome between the two subpopulations. Cluster 1 infections were more likely to be associated with peri-domestic activities while cluster 2 were associated with interior forest activities consistent with the preferred habitats of the respective macaque hosts. Infections with both P. knowlesi subpopulations cause a wide spectrum of disease including potentially life-threatening complications, with no implications for differential patient management.

Matched MeSH terms: Plasmodium knowlesi/growth & development
A comparison of weed communities of coastal rice fields in Peninsular Malaysia

Hakim MA, Juraimi AS, Hanafi MM, Selamat A

J Environ Biol, 2013 Sep;34(5):847-56.
PMID: 24558797

A survey was conducted at 100 different rice fields in coastal areas of West Malaysia to identify most common and prevalent weeds associated with rice. Fields surveyed were done according to the quantitative survey method by using 0.5m x 0.5m size quadrate with 20 samples from each field. A total of 53 different weed species belong to 18 families were identified of which 32 annual and 21 perennial; 12 grassy, 13 sedges and 28 broadleaved weeds. Based on relative abundance the most prevalent and abundant weed species were selected in the coastal rice field. Among the 10 most abundant weed species, there were four grasses viz. Echinochloa crusgalli, Leptochloo chinensis, Echinochloo colona, Oryza sotivo L. (weedy rice).; four sedges viz. Fimbristylis miliacea, Cyperus iria, Cyperus difformis, Scirpus grossus and two broadleaved weeds viz. Sphenocleo zeylonica, Jussiaea linifolio. Leptochloa chinensis, E. crusgalli, F. miliocea, E. colona were more prevalent and abundant species out of the 10 most dominant weed species in the coastal rice field of Peninsular Malaysia.

Matched MeSH terms: Oryza/growth & development*; Plant Weeds/growth & development
A contribution to the knowledge of Orthoptera diversity from Peninsular Malaysia: Bukit Larut, Perak

Tan MK, Kamaruddin KN

Zootaxa, 2016 May 12;4111(1):21-40.
PMID: 27394894 DOI: 10.11646/zootaxa.4111.1.2

Bukit Larut is a hill station at the southern tip of the Bintang Range, Perak of Peninsular Malaysia. While the biodiversity of Bukit Larut has been previously documented, its entomofauna, including the Orthoptera, remains relatively unknown. A faunistic survey was conducted in 2015 as part of the continuous exploration of the highlands in Malay Peninsula. An annotated species list of 71 (24 Caelifera and 47 Ensifera) species of Orthoptera from ten families (five from each order) is presented here. While the coverage of lineages in the orthopteran phylogeny is well-represented, the diversity in Bukit Larut is dominated by the three main families: Acrididae, Gryllidae and Tettigoniidae. Eight new locality records for Bukit Larut and/or Peninsular Malaysia and potential new species awaiting description highlight that the orthopteran diversity in Bukit Larut is not exhaustive.

Matched MeSH terms: Animal Structures/growth & development; Orthoptera/growth & development
A developmental study on the appearance of tocopherols and tocotrienols in developing palm mesocarp (Elaeis guineensis)

Choo YM, Ma AN, Chuah CH, Khor HT, Bong SC

Lipids, 2004 Jun;39(6):561-4.
PMID: 15554155

The concentration of vitamin E isomers, namely, alpha-tocopherol (alpha-T), alpha-tocotrienol, gamma-tocotrienol, and delta-tocotrienol in palm mesocarp at 4, 8, 12, 16, and 20 wk after anthesis (WAA) were quantified using HPLC coupled with fluorescence detection. alpha-T was detected throughout the palm fruits' maturation process, whereas unsaturated tocotrienols were found only in ripe palm fruits. These developmental results indicate that tocotrienols are synthesized between 16 and 20 WAA.

Matched MeSH terms: Fruit/growth & development*; Cycadophyta/growth & development
A feline kidney cell line-based plaque assay for feline calicivirus, a surrogate for Norwalk virus

Bidawid S, Malik N, Adegbunrin O, Sattar SA, Farber JM

J Virol Methods, 2003 Feb;107(2):163-7.
PMID: 12505630

Feline calicivirus (FCV) has been used by researchers as a surrogate for Norwalk virus (NV), since they share a similar genomic organization, physicochemical characteristics, and are grouped in the same family, Caliciviridae. Unlike NV, however, FCV can grow in established cell lines and produce a syncytial form of cytopathic effect. In this report, we describe the development and standardization of a plaque assay for FCV using monolayers of an established line of feline kidney (CrFK) cells in 12-well cell culture plates. The assay method has demonstrated reproducibility, ease of performance and resulted in clear plaque zones, readable in 24 h after virus inoculation. The infectivity titre of the virus by this plaque assay agreed well with tissue culture infectious dose(50) (TCID(50)) determinations. The described plaque assay would be a valuable tool in conducting various quantitative investigations using FCV as a model for NV and Norwalk-like viruses (NLV).

Matched MeSH terms: Norwalk virus/growth & development*; Calicivirus, Feline/growth & development*

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