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Displaying publications 21 - 28 of 28 in total

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Copper complex derived from S-benzyldithiocarbazate and 3-acetylcoumarin induced apoptosis in breast cancer cell

Foo JB, Low ML, Lim JH, Lor YZ, Zainol Abidin R, Eh Dam V, et al.

Biometals, 2018 08;31(4):505-515.
PMID: 29623473 DOI: 10.1007/s10534-018-0096-4

Copper complexes have been widely studied for the anti-tumour application as cancer cells are reported to take up greater amounts of copper than normal cells. Preliminary study revealed that the newly synthesised copper complex [Cu(SBCM)2] displayed marked anti-proliferative towards triple-negative MDA-MB-231 breast cancer cells. Therefore, Cu(SBCM)2 has great potential to be developed as an agent for the management of breast cancer. The present study was carried out to investigate the mode of cell death induced by Cu(SBCM)2 towards MDA-MB-231 breast cancer cells. The inhibitory and morphological changes of MDA-MB-231 cells treated with Cu(SBCM)2 was determined by using MTT assay and inverted light microscope, respectively. The safety profile of Cu(SBCM)2 was also evaluated towards human dermal fibroblast (HDF) normal cells. Confirmation of apoptosis and cell cycle arrest were determined by flow cytometry analysis. The expression of p53, Bax, Bcl-2 and MMP2 protein were detected with western blot analysis. Cu(SBCM)2 significantly inhibited the growth of MDA-MB-231 cells in a dose-dependent manner with GI50 18.7 ± 3.06 µM. Indeed, Cu(SBCM)2 was less toxic towards HDF normal cells with GI50 31.8 ± 4.0 µM. Morphological study revealed that Cu(SBCM)2-treated MDA-MB-231 cells experienced cellular shrinkage, membrane blebbing, chromatin condensation and formation of apoptotic bodies, suggesting that Cu(SBCM)2 induced apoptosis in the cells, which was confirmed by Annexin-V/PI flow cytometry analysis. It was also found that Cu(SBCM)2 induced G2/M phase cell cycle arrest towards MDA-MB-231 cells. The induction of apoptosis and cell cycle arrest in the present study is possibly due to the down-regulation of the mutant p53 and MMP2 protein. In conclusion, Cu(SBCM)2 can be developed as a targeted therapy for the treatment of triple-negative breast cancer.

Matched MeSH terms: Thiocarbamates/pharmacology; Thiocarbamates/chemistry
Fulltext Satisfaction with amplification in daily life (SADL) in different types and degrees of hearing loss

Nursakinah Abd Ghani, Wan Ahmad Wan Aslynn

International Journal of Allied Health Sciences, 2019;3(4):933-938.
MyJurnal

Introduction: The aim of this study is to determine the satisfaction level of hearing aid users in different types and degrees of hearing loss using the satisfaction with amplification in daily life (SADL) questionnaire. Methods: 35 hearing aid users with different types of hearing loss who sought help from the International Islamic University, Malaysia (IIUM) Hearing and Speech Clinic at Jalan Hospital Campus, Kuantan, IIUM Medical Specialist Centre and private hearing aid centres were involved in this study. The data was collected from the participants who filled in the Malay SADL questionnaires. Results: Among the thirty-five (35) participants, there was no significant difference reported between the types of hearing loss and their satisfaction level in the SADL global score and each of the subscales. It was found that satisfaction level with the hearing aids may depend on several factors such as cosmetic preference, cost of hearing aid and audiologist competence. Meanwhile, in terms of degrees of hearing loss, 80% of the users were relatively satisfied with their hearing aids. Yet, there was no correlation between hearing aid users’ satisfaction level and their degrees of hearing loss for all subscales and SADL global score. Conclusions: This study indicates that although hearing aid users reported to be satisfied with their hearing aids; there are some who are not satisfied in terms of feedback, telephone use, cosmetic appearance and costs of hearing aid. The findings of this study can benefit the audiologists, as they would be able to use the information to help future patients understand and adapt to the use of the hearing aids better.

Matched MeSH terms: Thiocarbamates
Fulltext Assessment of traffic noise and the association with non auditory effect among shop lot workers in Kajang, Selangor

Mohd Zahiruddin Zukfali, Haliza Abdul Rahman

Malaysian Journal of Public Health Medicine, 2016;16(10):0-0.
MyJurnal

The objective of study is to determine traffic noise level and non-auditory effect among shop lot workers at Kajang Selangor. This cross sectional study was carried to study traffic noise exposure with annoyance and work performance level among shop lot workers in Jalan Mendaling, JalanTukang and Jalan Sulaiman at Kajang town, Selangor. This study involves 120 shop lot workers that exposed to the traffic noise during their working hours where they are randomly selected. Noise exposure was estimated using the Sound Level Meter for environmental noise. The traffic volume was recorded using video recorder and calculated using tally counter. One set questionnaire consist standard questionnaire was used to assess the annoyance level and work performance level among the respondents. Respondents were predominantly by male which are 94 and female, 26 respondents. The mean age of the respondent were ranged between 41 to 60 years old. Only 12.5% of respondent are ranged 21 until 30 years old. In total of 120 respondent, 54.2% of them are Chinese while Malay and India only 30% and 15.8% respectively. The result showed that the traffic noise level at study areas are exceeded the permissible sound limit of commercial and business area during daylight which is 70 dB(A). Regarding work performance, 94 respondents are having low work performance level and 82% of respondent high annoyance level during the exposure of traffic noise from four different sources which are noise from the traffic, speeding vehicle, high traffic volume and exhaust system. There is a significant relationship between traffic noise level with work performance level (p=0.001) and annoyance level (p=0.026). The average traffic noise (Laeq) level at Jalan Mendaling, Jalan Tukang and Jalan Sulaiman is 71.19 dB(A) which were high and exceeds permissible sound level from road traffic, commercial and business place at day time, 70 dB(A). The exposure from the traffic noise effect the annoyance level and work performance level among the shop lot worker. In order to reduce traffic noise exposure towards the shop lot workers, some recommendation are needed to control the traffic noise such as build a noise barrier, plant trees and also enforcement of legal requirement in noise level.

Matched MeSH terms: Thiocarbamates
A simple method for chromium speciation analysis in contaminated water using APDC and a pre-heated glass tube followed by HPLC-PDA

Salihu SO, Bakar NKA

Talanta, 2018 May 01;181:401-409.
PMID: 29426532 DOI: 10.1016/j.talanta.2018.01.041

In this study, a simple sample preparation method was developed for the determination of tri-and hexavalent chromium in water samples. It utilizes a pre-heated customized glass tube (CGT), to supply the heat energy required for the reaction of Cr(III) with ammonium pyrrolidinedithiocarbamate (APDC). The products of the Cr complexes, tris(1-pyrrolidinecarbodithioato)chromium(III) and bis(1-pyrrolidinecarbodithioato)[1-pyrrolidinecarbodithio(thioperoxoato)]chromium(III) were chromatographed with Shimadzu LC-20AT and Zobax Eclipse C18 (150mm × 4.6mm, 5µm) column using ACN: Water, (7:3, v/v) as the mobile phase. The concentration of Cr(III) ranged from 0.06mgL-1to 0.09mgL-1and that of Cr(VI) was between 0.02mgL-1to 0.04mgL-1in the samples. Percentage recoveries from spiked real samples were between 87% (tap water) to 110% (wastewater) for Cr(III) and 92% (pond water) to 117% (tap water) for Cr(VI). The limits of detection (LODs) were 0.0029mgL-1and 0.0014mg/L-1for Cr(III) Cr(VI) respectively. While the limits of quantitation (LOQs), were 0.0098mgL-1and 0.0047mgL-1for Cr(III) and Cr(VI) respectively. Method precision (RSD (%)) was 3.3% and 3.5% for Cr(III) and Cr(VI) respectively. The developed method was applied for the speciation analysis of chromium in drinking water, tap water, wastewater, river water, and pond water samples. Our findings proved the method is simple and inexpensive. The method was validated by the analysis of a certified reference material (CRM) SLRS-4. The percentage recovery and RSD(%) from the spiked CRM were 91% and 115% and 0.32% and 1.4% for Cr(III) and Cr(VI) respectively.

Matched MeSH terms: Thiocarbamates
Cytotoxicity of triphenyltin(IV) methyl- and ethylisopropyldithiocarbamate compounds in chronic myelogenus leukemia cell line (K-562)

Awang N, Kamaludin NF, Hamid A, Mokhtar NW, Rajab NF

Pak J Biol Sci, 2012 Sep 01;15(17):833-8.
PMID: 24163967

Studies on the discovery of new cancer treatment by using metal-based compounds such as tin (Sn) has now greatly being synthesized and evaluated to identify their effectiveness and suitability to be developed as a new anticancer drug.
APPROACH: This study was carried out to evaluate the cytotoxicity of triphenyltin(lV) methylisopropyldithiocarbamate (compound 1) and triphenyltin(IV) ethylisopropyldithiocarbamate (compound (2) on chronic myelogenus leukemia cells. The determination of their cytotoxicity (IC50) at different time of exposure and concentration was carried out through the employment of 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay.
RESULTS: The IC50 values obtained for compound 1 and 2 following treatment at 24, 48 and 72 h were 0.660, 0.223, 0.370 microM and 0.677, 0.306, 0.360 microM, respectively. Cell morphological changes such as apoptotic and necrotic features were also been observed.
CONCLUSION: The compounds tested were found to give cytotoxic effect against chronic myelogenus leukemia (K-562) cell at a micromolar dose. Thus, further study on their specific mechanism of actions in the human cells should be carried out to elucidate their potential as an anticancer agent.

Matched MeSH terms: Thiocarbamates/pharmacology*
Phosphanegold(I) dithiocarbamates, R3PAu[SC(=S)N((i)Pr)CH2CH2OH] for R = Ph, Cy and Et: role of phosphane-bound R substituents upon in vitro cytotoxicity against MCF-7R breast cancer cells and cell death pathways

Jamaludin NS, Goh ZJ, Cheah YK, Ang KP, Sim JH, Khoo CH, et al.

Eur J Med Chem, 2013 Sep;67:127-41.
PMID: 23856069 DOI: 10.1016/j.ejmech.2013.06.038

The synthesis and characterisation of R3PAu[S2CN((i)Pr)CH2CH2OH], for R = Ph (1), Cy (2) and Et (3)4, is reported. Compounds 1-3 are cytotoxic against the doxorubicin-resistant breast cancer cell line, MCF-7R, with 1 exhibiting greater potency and cytotoxicity than either of doxorubicin and cisplatin. Based on human apoptosis PCR-array analysis, caspase activities, DNA fragmentation, cell apoptotic assays, intracellular reactive oxygen species (ROS) measurements and human topoisomerase I inhibition, induction of apoptosis by 1, and necrosis by 2 and 3, are demonstrated, by both extrinsic and intrinsic pathways. Compound 1 activates the p53 gene, 2 activates only the p73 gene, whereas 3 activates both the p53 and p73 genes. Compounds 1 and 3 activate NF-κB, and each inhibits topoisomerase I.

Matched MeSH terms: Thiocarbamates/chemistry*
Two new monofunctional platinum(II) dithiocarbamate complexes: phenanthriplatin-type axial protection, equatorial-axial conformational isomerism, and anticancer and DNA binding studies

Imran M, Rehman ZU, Hogarth G, Tocher DA, Chaudhry GE, Butler IS, et al.

Dalton Trans, 2020 Nov 10;49(43):15385-15396.
PMID: 33140800 DOI: 10.1039/d0dt03018j

The syntheses of two platinum(ii) dithiocarbamate complexes (1 and 2) that show quinoplatin- and phenanthriplatin-type axial protection of the Pt-plane are described. The Pt-plane of complex 2 is axially more protected than that of complex 1. Furthermore, both complexes adopt two different stereochemical conformations in the solid state (based on single-crystal X-ray structures) owing to the structurally flexible piperazine backbone; i.e., C-e,e-Anti (1) and C-e,a-Syn (2), where "C" stands for the chair configuration, "e" and "a" stand for the equatorial and axial positions and "Anti" (opposite side) and "Syn" (same side) represent the relative orientations in space of the terminal substituents on the piperazine ring. In complex 2, the C-e,a-Syn conformation may provide additional steric hindrance to the Pt-plane. Despite the lower lipophilicity of 2 as compared to that of 1, the in vitro anticancer action against selected cancer cell lines is better for the former revealing the superior role of the axial protection over lipophilicity in modulating anticancer activity. The activity against the cancer promoting protein NF-κB signifies that the mode of cancer cell death may be the result of hindering the activity of NF-κB in the initiation of apoptosis. The apoptotic mode of cell death has been established earlier in a study using Annexin V-FITC. Finally, DNA binding studies revealed that the complex-DNA adduct formation is spontaneous and the mode of interaction is non-intercalative (electrostatic/covalent).

Matched MeSH terms: Thiocarbamates/chemistry*
Our recent experiences with sarin poisoning cases in Japan and pesticide users with references to some selected chemicals

Yokoyama K

Neurotoxicology, 2007 Mar;28(2):364-73.
PMID: 16730798

Attention has been paid to neurobehavioral effects of occupational and environmental exposures to chemicals such as pesticides, heavy metals and organic solvents. The area of research that includes neurobehavioral methods and effects in occupational and environmental health has been called "Occupational and Environmental Neurology and Behavioral Medicine." The methods, by which early changes in neurological, cognitive and behavioral function can be assessed, include neurobehavioral test battery, neurophysiological methods, questionnaires and structured interview, biochemical markers and imaging techniques. The author presents his observations of neurobehavioral and neurophysiological effects in Tokyo subway sarin poisoning cases as well as in pesticide users (tobacco farmers) in Malaysia in relation to Green Tobacco Sickness (GTS). In sarin cases, a variety effects were observed 6-8 months after exposure, suggesting delayed neurological effects. Studies on pesticide users revealed that organophosphorus and dithiocarbamate affected peripheral nerve conduction and postural balance; subjective symptoms related to GTS were also observed, indicating the effects of nicotine absorbed from wet tobacco leaves. In addition, non-neurological effects of pesticides and other chemicals are presented, in relation to genetic polymorphism and oxidative stress.

Matched MeSH terms: Thiocarbamates/adverse effects