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Fulltext Effect of nutritional factors on the growth and production of biosurfactant by Pseudomonas aeruginosa strain 181

Al-Araji, L., Rahman, R.N.Z.A., Basri, M., Salleh, A.B.

ASM Science Journal, 2008;2(1):45-56.
MyJurnal

The growth and production of biosurfactant by P. seudomonas aeruginosa (181) was dependant on nutritional factors. Among the eleven carbon sources tested, glucose supported the maximum growth (0.25 g/L) with the highest biosurfactant yield and this was followed by glycerol. Glucose reduced the surface tension to 35.3 dyne/cm and gave an E24 reading of 62.7%. Butanol gave the lowest growth and had no biosurfactant production. For the nitrogen sources tested, casamino acid supported a growth of 0.21 g/L which reduced the surface tension to 41.1 dyne/cm and gave an E24 reading of 56%. Soytone was assimilated similarly, with good growth and high biosurfactant production. Corn steep liquor gave the lowest growth and did not show any biosurfactant activity.

Matched MeSH terms: Reading
Fulltext Continuing Education and the Malaysian Dentists

Razak, I.A.

Ann Dent, 1995;2(1):-.
MyJurnal

A 73.1 percent response rate was obtained in a postal questionnaire survey conducted among Malaysian dentists to assess their attitudes and needs for continuing dental education. It appeared that on an average the Malaysian dentist spent very little time on continuing education,reading journals and participation in professional dental meetings.The need for continuing education was strongly evident as almost all dentists indicated that such activities be further developed in Malaysia. Crown and Bridgework, Oral Surgery and Orthodontic appeared to be areas in which more continuing education were required.

Matched MeSH terms: Reading
Fulltext A research review: how technology helps to improve the learning process of learners with dyslexia

Chai JT, Chen CJ

Journal of Cognitive Sciences & Human Development, 2017;2(2):26-43.
MyJurnal

Dyslexia is a language disorder that leads to difficulty with words and it is the most common type of learning disability. This article presents a systematic review on the current state of assistive technologies used in improving the learning process of learn-ers with dyslexia. A total of 25 journals articles and international conference papers published between 2000 and 2014 were included in the review. The research articles were collected from 12 databases and analyzed based on the qualitative cyclical pro-cess. A majority of the studies focused on children and adolescents. Four main themes on the types of technologies used in aiding the learning process of learners with dys-lexia are derived and discussed. These include text-to-speech, eye-tracking, virtual learning environments, and games. The text-to-speech technology is the most common type of technology used by learners with dyslexia. In terms of the roles played by the assistive technologies, another four emerging themes are identified, which cover the roles of aiding reading, writing, memory, and mathematics. The review also discovers that a majority of these studies focus on the use of technologies for improving the reading ability of learners with dyslexia.

Matched MeSH terms: Reading
Fulltext Genome Sequence of Bacillus sp. Strain UMTAT18 Isolated from the Dinoflagellate Alexandrium tamiyavanichii Found in the Straits of Malacca

Danish-Daniel M, Ming GH, Mohd Noor ME, Sung YY, Usup G

Genome Announc, 2016 Oct 6;4(5).
PMID: 27795265 DOI: 10.1128/genomeA.01106-16

Bacillus sp. strain UMTAT18 was isolated from the harmful dinoflagellate Alexandrium tamiyavanichii Its genome consists of 5,479,367 bp with 5,546 open reading frames, 102 tRNAs, and 29 rRNAs. Gene clusters for biosynthesis of nonribosomal peptides, bacteriocin, and lantipeptide were identified. It also contains siderophore and genes related to stress tolerance.

Matched MeSH terms: Open Reading Frames
Effect of scanning parameters on dose-response of radiochromic films irradiated with photon and electron beams

Tagiling N, Ab Rashid R, Azhan SNA, Dollah N, Geso M, Rahman WN

Heliyon, 2018 Oct;4(10):e00864.
PMID: 30364574 DOI: 10.1016/j.heliyon.2018.e00864

Proper dosimetry settings are crucial in radiotherapy to ensure accurate radiation dose delivery. This work evaluated scanning parameters as affecting factors in reading the dose-response of EBT2 and EBT3 radiochromic films (RCFs) irradiated with clinical photon and electron beams. The RCFs were digitised using Epson® Expression® 10000XL flatbed scanner and image analyses of net optical density (netOD) were conducted using five scanning parameters i.e. film type, resolution, image bit depth, colour to grayscale transformation and image inversion. The results showed that increasing spatial resolution and deepening colour depth did not improve film sensitivity, while grayscale scanning caused sensitivity reduction below than that detected in the Red-channel. It is also evident that invert and colour negative film type selection negated netOD values, hence unsuitable for scanning RCFs. In conclusion, choosing appropriate scanning parameters are important to maintain preciseness and reproducibility in films dosimetry.

Matched MeSH terms: Reading
Fulltext Crystal structure and functional analysis of human C1ORF123

A Rahaman SN, Mat Yusop J, Mohamed-Hussein ZA, Aizat WM, Ho KL, Teh AH, et al.

PeerJ, 2018;6:e5377.
PMID: 30280012 DOI: 10.7717/peerj.5377

Proteins of the DUF866 superfamily are exclusively found in eukaryotic cells. A member of the DUF866 superfamily, C1ORF123, is a human protein found in the open reading frame 123 of chromosome 1. The physiological role of C1ORF123 is yet to be determined. The only available protein structure of the DUF866 family shares just 26% sequence similarity and does not contain a zinc binding motif. Here, we present the crystal structure of the recombinant human C1ORF123 protein (rC1ORF123). The structure has a 2-fold internal symmetry dividing the monomeric protein into two mirrored halves that comprise of distinct electrostatic potential. The N-terminal half of rC1ORF123 includes a zinc-binding domain interacting with a zinc ion near to a potential ligand binding cavity. Functional studies of human C1ORF123 and its homologue in the fission yeast Schizosaccharomyces pombe (SpEss1) point to a role of DUF866 protein in mitochondrial oxidative phosphorylation.

Matched MeSH terms: Open Reading Frames
Fulltext Analysis the influence of heart rate and muscle activity towards muscle fatique in layup workers

Noor'Ain Azizan, Seri Rahayu Kamat, Nur Syafiqah Rayme, Ruzy Haryati Hambali

Journal of Advanced Research in Applied Sciences and Engineering Technology, 2018;11(1):99-107.
MyJurnal

Repetitive movement can lead to the pain muscle, nerves, and tendons that cause by
repetitive overuse of working task. The muscle will fatigue due to; repetitive
movement, force that been applied, posture during working and duration of working.
The stress level during working can influent the energy performance usage during
working. The aim of this paper is to analyse the influence of heart rate and muscle
activity of workers in composite manufacturing towards muscle fatigue. The data was
collected for a worker in hand layup department and the Qualitative method was used
in a way to investigate the working load and level of pain received by their body. Then,
the Qualitative data was sorted and the respondent proceeded for a Quantitative
method which involves muscle activity analysis and heart rate analysis. The tools that
were used to conduct these experiments were surface electromyography (sEMG),
Wristwatch with chest strap and perceived stress scale (PSS). The experimentation
used to calculate the average reading of heart rate and muscle activity during working
and detect the duration the muscle to start fatigue. Moreover, this paper analysed the
relationship between heart rate and muscle activity through the duration of working.
As an overall finding of this research, it was shown that the heart rate of the workers
influence the muscle activity of workers and has high potential relationship to the
fatigue of muscles of the workers in the layup department.

Matched MeSH terms: Reading
Fulltext Ambit determination method in estimating rice plant population density

Abu Bakar, B., Abdul Rahman, M.S., Teoh, C.C., Abdullah, M.Z.K., Ismail, R.

Food Research, 2018;2(2):177-182.
MyJurnal

Rice plant population density is a key indicator in determining the crop setting and fertilizer application rate. It is therefore essential that the population density is monitored to ensure that a correct crop management decision is taken. The conventional method of determining plant population is by manually counting the total number of rice plant tillers in a 25 cm x 25 cm square frame. Sampling is done by randomly choosing several different locations within a plot to perform tiller counting. This sampling method is time consuming, labour intensive and costly. An alternative fast estimating method was developed to overcome this issue. The method relies on measuring the outer circumference
or ambit of the contained rice plants in a 25 cm x 25 cm square frame to determine the number of tillers within that square frame. Data samples of rice variety MR219 were collected from rice plots in the Muda granary area, Sungai Limau Dalam, Kedah. The data were taken at 50 days and 70 days after seeding (DAS). A total of 100 data samples were collected for each sampling day. A good correlation was obtained for the variety of 50 DAS and 70 DAS. The model was then verified by taking 100 samples with the latching strap for 50 DAS and 70 DAS. As a result, this technique can be used as a fast, economical and practical alternative to manual tiller counting. The technique can potentially be used in the development of an electronic sensing system to estimate paddy plant population density.

Matched MeSH terms: Reading Frames
Fulltext Gauging The Enemy in the Blanket through the paradigm of Malayness

Nurhanis Sahiddan, Haizan Mohd Taha, Mastura Johar

Malaysian Journal of Social Sciences and Humanities, 2016;1(2):1-8.
MyJurnal

Anthony Burgess (1917 - 1993) has written a trilogy of novels on the Malay World, namely The
Malayan Trilogy (1964). It has been suggested that the trilogy, which consists of the novels Time for a
Tiger (1956), The Enemy in the Blanket (1958) and Beds in the East (1959), depicts the Islamic
practices through its Muslim Malay characters, displaying their hypocrisy and their wayward Islamic
practices as stated by Zawiyah Yahya (2003). In contrast, the trilogy has rarely been studied based on
elements of the paradigm of Malayness in literature, consisting of six elements, namely the Malay
language, Islam, the Malay rulers, adat/culture, ethnicity, and identity. Therefore, we aim to analyse
one of the novels in the trilogy, The Enemy in the Blanket, in light of Islam as one of the elements
under the paradigm of Malayness in literature as stated by Ida as our primary conceptual framework in
this study. To achieve these objectives, we employ a close textual reading on the novel by analysing
the Muslim Malay characters in The Enemy in the Blanket. The findings show that the Muslim Malay
characters in The Enemy in the Blanket could be considered as wayward Muslims in their practices and
beliefs. Therefore, we would like to reconfirm that the paradigm of Malayness in in fact, an everydaydefined
social reality, as experienced by the people in the course of their everyday life as opposed to it
as an authority-defined social reality, as defined by people of the dominant power structure. It is hoped
that this study will contribute to the on-going discourse on Islam as the paradigm of Malayness as well
as English literature on the Malay World.

Matched MeSH terms: Reading
Fulltext Reading Performance and Compensatory Head Posture in Infantile Nystagmus after Null Zone Training

Mohamad Fadzil N, Mohammed Z, Mohamad Shahimin M, Saliman NH

Int J Environ Res Public Health, 2019 11 27;16(23).
PMID: 31783492 DOI: 10.3390/ijerph16234728

This study aimed to assess the visual function, reading performance, and compensatory head posture (CHP) in schoolchildren with infantile nystagmus. A total of 18 participants aged between 13 to 18 years old were divided into spectacle (n = 9) and null zone group (n = 9) based on their visual acuity. Visual acuity (LogMAR), contrast sensitivity (Pelli-Robson), reading time and rate (Tobii TX300), and CHP were measured pre and post null zone reading training. Participants in the null zone group received 10 sessions of training (5 weeks). Visual acuity and contrast sensitivity of participants in the spectacle and null zone groups were not significantly different pre and post training. Reading performance, i.e., reading time (z = -1.36; p = 0.173) and reading rate (z = -0.06; p = 0.953), of participants in the spectacle group was not significantly different after 5 weeks. Reading time (z = -2.55; p = 0.011) and reading rate (z = -2.07; p = 0.038 of participants in the null zone group showed significant improvement post training. After 5 weeks, CHP improved in six out of the nine participants (66.7%) of the null zone group and was unchanged in all participants in the spectacle group. Null zone reading training could benefit children with infantile nystagmus in improving reading performance and compensatory head posture.

Matched MeSH terms: Reading
Fulltext Allah’s wondrous creatures, the holy Qur’an and technological inventions: ultrasound imaging

Sayed, Inayatullah Shah

International Medical Journal Malaysia, 2018;17(101):83-88.
MyJurnal

Allah (s.w.t) has created innumerable distinct creatures and mentioned to us about their special qualities through His revelation. The Qur’an is the ultimate source of guidance for its followers for all aspects of life including science. If one is to study nature scientifically there are countless observable facts that are parallel to the teachings of Islam. One of these facts is echolocation found in bats and dolphins. These animals generate ultrasonic signals and detect the echoes reflected back to them to map out their environment and catch prey. Modern health sciences have already adopted this phenomenon in the form of ultrasound imaging for diagnosis of certain diseases. However, there is room for improvement in the overall performance of this technique. This article highlights the technological developments directly inspired by nature i.e., crawfish/crayfish and relates echolocation characteristics of bats and dolphins with basic principles of ultrasound imaging. In-depth studies on the echolocation properties of these creatures can lead to further improvement in the current ultrasound imaging technique. Such as; the construction of a transducer which simultaneously generates multi-frequency ultrasound signals and development of new interpreting software. Moreover, reading verses of the Holy Qur’an heartily and enthusiastically will lead to the development of innovative ideas that can be translated into reality and applied for the betterment of humankind.

Matched MeSH terms: Reading
Fulltext Effect of windscreen glass waste powder (WGWP) mortar on water absorption

Nik Anisah Nik Ngah, Maziah Muda, Ahmad Nasrul Hisyam Hamzah, Mohamad Awang, Kartini Kamaruddin

Journal of Engineering and Science Research, 2018;2(4):7-11.
MyJurnal

Diverting waste material from landfill sites has a big implication for the creation of new markets and has environmental benefits through offsetting the need for the extraction of raw materials. Besides, the major attention especially in construction industry is the problem to make natural resources sustainable for three to four decades in future. However, in this study finely glass powder with pozzolanic properties is found to be a partial replacement for cement and can acts as filler in the microstructure of a mortar block. Thus, a study was conducted to see the effects of various proportions of windscreen glass waste powder (WGWP) in determining the degree of water absorption on mortar incorporated with WGWP and to determine the optimum replacement to the ordinary Portland cement (OPC) with WGWP. Several compositions of WGWP (0%, 5%, 10%, 15% and 20% by cement weight) of mortar cube sample were prepared and cured at 7, 28 and 60 days to undergo a water absorption test. From this research, it shows that the use of WGWP was found to be the best features pozzolanic enough to use as a partial replacement for cement and it can reduced the pores of mortar by blocking the large voids in the hydrated cement paste through pozzolanic reaction. In the term of water absorption, the lowest reading was achieved in 15% and it can be said that 15% replacement is the optimum replacement of OPC.

Matched MeSH terms: Reading
Fulltext Psychrobacter Phage Encoding an Antibiotics Resistance Gene Represents a Novel Caudoviral Family

Wang H, Ren L, Liang Y, Zheng K, Guo R, Liu Y, et al.

Microbiol Spectr, 2023 Aug 17;11(4):e0533522.
PMID: 37272818 DOI: 10.1128/spectrum.05335-22

Psychrobacter is an important bacterial genus that is widespread in Antarctic and marine environments. However, to date, only two complete Psychrobacter phage sequences have been deposited in the NCBI database. Here, the novel Psychrobacter phage vB_PmaS_Y8A, infecting Psychrobacter HM08A, was isolated from sewage in the Qingdao area, China. The morphology of vB_PmaS_Y8A was characterized by transmission electron microscopy, revealing an icosahedral head and long tail. The genomic sequence of vB_PmaS_Y8A is linear, double-stranded DNA with a length of 40,226 bp and 44.1% G+C content, and encodes 69 putative open reading frames. Two auxiliary metabolic genes (AMGs) were identified, encoding phosphoadenosine phosphosulfate reductase and MarR protein. The first AMG uses thioredoxin as an electron donor for the reduction of phosphoadenosine phosphosulfate to phosphoadenosine phosphate. MarR regulates multiple antibiotic resistance mechanisms in Escherichia coli and is rarely found in viruses. No tRNA genes were identified and no lysogeny-related feature genes were detected. However, many similar open reading frames (ORFs) were found in the host genome, which may indicate that Y8A also has a lysogenic stage. Phylogenetic analysis based on the amino acid sequences of whole genomes and comparative genomic analysis indicate that vB_PmaS_Y8A contains a novel genomic architecture similar only to that of Psychrobacter phage pOW20-A, although at a low similarity. vB_PmaS_Y8A represents a new family-level virus cluster with 22 metagenomic assembled viral genomes, here named Minviridae. IMPORTANCE Although Psychrobacter is a well-known and important bacterial genus that is widespread in Antarctic and marine environments, genetic characterization of its phages is still rare. This study describes a novel Psychrobacter phage containing an uncharacterized antibiotic resistance gene and representing a new virus family, Minviridae. The characterization provided here will bolster current understanding of genomes, diversity, evolution, and phage-host interactions in Psychrobacter populations.

Matched MeSH terms: Open Reading Frames
Fulltext Shewanella phage encoding a putative anti-CRISPR-like gene represents a novel potential viral family

Wang H, Zheng K, Wang M, Ma K, Ren L, Guo R, et al.

Microbiol Spectr, 2024 Feb 06;12(2):e0336723.
PMID: 38214523 DOI: 10.1128/spectrum.03367-23

Shewanella is a prevalent bacterial genus in deep-sea environments including marine sediments, exhibiting diverse metabolic capabilities that indicate its significant contributions to the marine biogeochemical cycles. However, only a few Shewanella phages were isolated and deposited in the NCBI database. In this study, we report the isolation and characterization of a novel Shewanella phage, vB_SbaS_Y11, that infects Shewanella KR11 and was isolated from the sewage in Qingdao, China. Transmission electron microscopy revealed that vB_SbaS_Y11 has an icosahedral head and a long tail. The genome of vB_SbaS_Y11 is a linear, double-stranded DNA with a length of 62,799 bp and a G+C content of 46.9%, encoding 71 putative open reading frames. No tRNA genes or integrase-related feature genes were identified. An uncharacterized anti-CRISPR AcrVA2 gene was detected in its genome. Phylogenetic analysis based on the amino acid sequences of whole genomes and comparative genomic analyses indicate that vB_SbaS_Y11 has a novel genomic architecture and shares low similarity to Pseudomonas virus H66 and Pseudomonas phage F116. vB_SbaS_Y11 represents a potential new family-level virus cluster with eight metagenomic assembled viral genomes named Ranviridae.IMPORTANCEThe Gram-negative Shewanella bacterial genus currently includes about 80 species of mostly aquatic Gammaproteobacteria, which were isolated around the globe in a multitude of environments, such as freshwater, seawater, coastal sediments, and the deepest trenches. Here, we present a Shewanella phage vB_SbaS_Y11 that contains an uncharacterized anti-CRISPR AcrVA2 gene and belongs to a potential virus family, Ranviridae. This study will enhance the knowledge about the genome, diversity, taxonomic classification, and global distribution of Shewanella phage populations.

Matched MeSH terms: Open Reading Frames
Fulltext Age factor affects reading acuity and reading speed in attaining text information

Chen AH, Khalid NM, Buari NH

Int J Ophthalmol, 2019;12(7):1170-1176.
PMID: 31341810 DOI: 10.18240/ijo.2019.07.19

AIM: To investigate the effect of age on reading acuity and reading speed in attaining text information in healthy eyes.
METHODS: Reading acuity, critical print size, reading speed and maximum reading speed were measured in groups of 40 children (8 to 12 years old), 40 teenagers (13 to 19 years old), 40 young adults (20 to 39 years old), and 40 adults (40 years old and above) using the Buari-Chen Malay Reading Chart [contextual sentences (CS) set and random words (RW) set] in a cross-sectional study design.
RESULTS: Reading acuity was significantly improved by 0.04 logMAR for both CS set and RW set from children to teenagers, then gradually worsened from young adults to adults (CS set: 0.06 logMAR; RW set: 0.08 logMAR). Critical print size for children showed a significant improvement in teenagers (CS set: 0.14 logMAR; RW set: 0.07 logMAR), then deteriorated from young adults to adults by 0.09 logMAR only for CS set. Reading speed significantly increased from children to teenagers, [CS set: 46.20 words per minute (wpm); RW set: 42.06 wpm], then stabilized from teenagers to young adults, and significantly reduced from young adults to adults (CS set: 28.58 wpm; RW set: 24.44 wpm). Increment and decrement in maximum reading speed measurement were revealed from children to teenagers (CS set: 39.38 wpm; RW set: 43.38 wpm) and from young adults to adults (CS set: 22.26 wpm; RW set: 26.31 wpm) respectively.
CONCLUSION: The reference of age-related findings in term of acuity and speed of reading should be incorporated in clinical practice to enhance reading assessment among healthy eyes population.

Matched MeSH terms: Reading
Haplotype data of mitochondrial DNA coding region encompassing nucleotide positions 11,719-12,184 and evaluate the importance of these positions for forensic genetic purposes in Iraq

Hameed IH, Jebor MA, Ommer AJ, Abdulzahra AI, Yoke C

Mitochondrial DNA A DNA Mapp Seq Anal, 2016;27(2):1324-7.
PMID: 25090379 DOI: 10.3109/19401736.2014.945576

Samples of 100 random healthy unrelated Iraqi male persons from the Arab ethnic group of Iraqi population were collected for mtDNA coding region sequencing by using the Sanger technique and to establish the degree of variation characteristic of a fragment. Portion of coding region encompassing positions 11,719-12,184 was amplified in accordance with the Anderson reference sequence. PCR products were purified by EZ-10 spin column then sequenced and detected by using the ABI 3130xL DNA Analyzer. This is to intend the detection of polymorphisms of mtDNA. Four new polymorphic positions 11,741, 11,756, 11,878, and 12,133 are described which may be suitable in the future to be the sources for human identification purpose in Iraq. The obtained data can be used to identify variable nucleotide positions characterized by frequent occurrence most promising for identification variants. The calculated value GD = 0.95 and RMP = 0.048 of the genetic diversity should be understood as high in the context of coding function of the analysed DNA fragment. The relatively high gene diversity and a relatively low random match probability were observed in this study.

Matched MeSH terms: Open Reading Frames*
Acquisition of Malay word recognition skills: lessons from low-progress early readers

Lee LW, Wheldall K

Dyslexia, 2011 Feb;17(1):19-37.
PMID: 21241030 DOI: 10.1002/dys.421

Malay is a consistent alphabetic orthography with complex syllable structures. The focus of this research was to investigate word recognition performance in order to inform reading interventions for low-progress early readers. Forty-six Grade 1 students were sampled and 11 were identified as low-progress readers. The results indicated that both syllable awareness and phoneme blending were significant predictors of word recognition, suggesting that both syllable and phonemic grain-sizes are important in Malay word recognition. Item analysis revealed a hierarchical pattern of difficulty based on the syllable and the phonic structure of the words. Error analysis identified the sources of errors to be errors due to inefficient syllable segmentation, oversimplification of syllables, insufficient grapheme-phoneme knowledge and inefficient phonemic code assembly. Evidence also suggests that direct instruction in syllable segmentation, phonemic awareness and grapheme-phoneme correspondence is necessary for low-progress readers to acquire word recognition skills. Finally, a logical sequence to teach grapheme-phoneme decoding in Malay is suggested.

Matched MeSH terms: Reading*
Contributions of phonological awareness, phonological short-term memory, and rapid automated naming, toward decoding ability in students with mild intellectual disability

Soltani A, Roslan S

Res Dev Disabil, 2013 Mar;34(3):1090-9.
PMID: 23314249 DOI: 10.1016/j.ridd.2012.12.005

Reading decoding ability is a fundamental skill to acquire word-specific orthographic information necessary for skilled reading. Decoding ability and its underlying phonological processing skills have been heavily investigated typically among developing students. However, the issue has rarely been noticed among students with intellectual disability who commonly suffer from reading decoding problems. This study is aimed at determining the contributions of phonological awareness, phonological short-term memory, and rapid automated naming, as three well known phonological processing skills, to decoding ability among 60 participants with mild intellectual disability of unspecified origin ranging from 15 to 23 years old. The results of the correlation analysis revealed that all three aspects of phonological processing are significantly correlated with decoding ability. Furthermore, a series of hierarchical regression analysis indicated that after controlling the effect of IQ, phonological awareness, and rapid automated naming are two distinct sources of decoding ability, but phonological short-term memory significantly contributes to decoding ability under the realm of phonological awareness.

Matched MeSH terms: Reading*
Levels of Visual Stress in Proficient Readers: Effects of Spectral Filtering of Fluorescent Lighting on Reading Discomfort

Loew SJ, Rodríguez C, Marsh NV, Jones GL, Núñez JC, Watson K

Span J Psychol, 2015;18:E58.
PMID: 26255657 DOI: 10.1017/sjp.2015.59

Visual stress (VS) affects reading in 5-12% of the general population and 31-36% of children with reading disorders. Symptoms include print distortions and visual discomfort when reading, and are exacerbated by fluorescent lighting. Prior research has indicated that VS can also affect proficient readers. We therefore examined levels of visual discomfort in a group of expert readers (n = 24) under both standard and spectrally-filtered fluorescent lighting. Participants rated their awareness of six symptoms of VS under each lighting condition. Under the standard condition, 4(16.7%) of the group recorded moderate to high levels of VS. Differences in symptom levels and reading speed between conditions were analysed using the Wilcoxon Signed Rank Test. Under the filter condition, the group reported less discomfort regarding all six symptoms of VS surveyed. The differences were significant with respect to three of the symptoms (p = .029 - p < .001), with a medium effect size in all of them (r = .31 - r = .46) and total score (p = .007; r = .39). Variations in reading proficiency included significantly fewer self-corrections (p = .019) and total errors (p = .004). Here we present evidence that VS-type symptoms of reading discomfort are not confined to populations with reading difficulties and may also occur in proficient readers, and that simple adaptations to fluorescent lighting may alleviate such symptoms.

Matched MeSH terms: Reading*
Rice tungro bacilliform virus open reading frame 3 encodes a single 37-kDa coat protein

Marmey P, Bothner B, Jacquot E, de Kochko A, Ong CA, Yot P, et al.

Virology, 1999 Jan 20;253(2):319-26.
PMID: 9918890

Rice tungro bacilliform virus (RTBV) is a plant pararetrovirus and a member of the Caulimoviridae family and closely related to viruses in the Badnavirus genus. The coat protein of RTBV is part of the large polyprotein encoded by open reading frame 3 (ORF3). ORF3 of an RTBV isolate from Malaysia was sequenced (accession no. AF076470) and compared with published sequences for the region that encodes the coat protein or proteins. Molecular mass of virion proteins was determined by mass spectrometry (matrix-assisted laser desorption/ionization-TOF) performed on purified virus particles from three RTBV isolates from Malaysia. The N- and C-terminal amino acid sequences of the coat protein were deduced from the mass spectral analysis, leading to the conclusion that purified virions contain a single coat protein of 37 kDa. The location of the coat protein domain in ORF3 was reinforced as a result of immunodetection reactions using antibodies raised against six different segments of ORF3 using Western immunoblots after SDS-PAGE and isoelectrofocusing of proteins purified from RTBV particles. These studies demonstrate that RTBV coat protein is released from the polyprotein as a single coat protein of 37 kDa.

Matched MeSH terms: Open Reading Frames*

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