The present study was conducted to investigate the effect of oyster mushroom (Pleurotus sajorcaju,
PSC) addition to partially replace coconut milk powder on nutritional composition and
sensory values of Herbal Seasoning (HS). This study evaluates the nutritional composition,
dietary fibre and sensory acceptance of HS that processed using six different formulations
with different levels of PSC powder, namely 0% (A), 20% (B), 40% (C), 60% (D), 80% (E)
and 100% (F). The use of PSC powder substantially brought down the fat content of HS.
The fat content of PSC-based HS was ranged from 13.82±0.84% to 8.16±0.74%. The protein
content showed an increasing trend in line with increasing of PSC powder ranging from 7%
to 12%.Substitution of coconut milk powder with PSC powder resulted in significantly higher
(p0.05).The panels preferred HS formulated with PSC powder since its
enhance colour and viscosity attributes of the products. In brief, HS formulated with more than
40% PSC powder is recommended since it has significant nutrients and palatably accepted by
sensorial panellists.
Consumption of dietary fibre-rich food has been associated with various beneficial physiological properties and health effects. Presently, different types of natural fibre-rich ingredients are added into variety of bakery-based products to improve it fibre content for health promotional purposes. However, majority of these products are frequently added with imported dietary fibre ingredients. In the present study, bread samples were prepared with Zea mays ear (young corn) powder at the levels of either 2%, 4% or 6%. The bread samples were analyzed for nutritional composition, textural properties and sensory acceptance. Addition of young corn powder (YCP) at the level of 6% increases total dietary fibre (from 3.48% to 5.26%), moisture (from 25.64% to 26.55%), fat (from 4.35% to 4.61%) and protein content (from 9.13% to 9.51%) significantly. However, with the increasing of YCP levels in the bread, the carbohydrate content was decreased significantly (from 59.93% to 58.34%) while the ash content (from 0.95% to 0.99%) was not significantly affected. Results of texture profile analysis indicated that addition of YCP up to 6% not significantly affected the springiness (1.01-1.00) but significantly decreased cohesiveness (0.95-0.82). However, the addition of YCP up to 6% has increased hardness (0.18kg-0.57kg), gumminess (0.17kg-0.47kg) and chewiness (0.18kg-0.47kg) attributes of bread samples. On the other result, the sensory evaluation shows that the flavour score was not significantly affected by addition of YCP up to 4% (4.82-4.52) while the tenderness (4.53-4.42), elasticity (4.75-4.58), aroma (4.40-4.47), colour (4.93-4.55) and overall acceptance (4.80-4.35) scores were not significant affected up to 6%. In summary, breads with 4% addition of YCP were considered to be acceptable and potentially used in improving nutritional composition without changing sensory score.
Water kefir is the product of a brown sugar solution fermented with water kefir grains. To produce starter cultures for manufacturing commercial water kefir on an industrial scale, six lactic acid bacteria (LAB), three acetic acid bacteria (AAB), and two yeast strains have been isolated from local water kefir grains, and in vitro α-glucosidase inhibitory activity has been investigated. Five of the 11 isolates (LAB [K65, K9, K67], AAB [A5], and yeast [Y6]) showed high α-glucosidase inhibitory activity. They were then tested in an in vitro gastrointestinal tract tolerance test in which all demonstrated tolerance (40‒80% survival rate). These five strains, identified as Lactobacillus mali, Lactobacillus casei, Leuconostoc mesenteroides, Gluconobacter hansenii and Saccharomyces cerevisiae; were then used to ferment water kefir beverages. The storage stability of the fermented beverage products was analysed during 28 days of storage at 4°C by measuring α-glucosidase inhibitory activity, microbial cell viability, and microbial survival in the beverage treated with simulated gastric juice. The water kefir beverage sample containing both pumpkin purée and brown sugar fermented using all five strains (109 CFU/mL LAB strains, 107 CFU/mL yeast strain, and 106 CFU/mL AAB strain) was the most suitable formulation because it achieved >50% α-glucosidase inhibitory activity and microbial survival rates of 106‒1010 CFU/mL viable LAB cells, 106‒107 CFU/mL viable AAB cells, and 105‒108 CFU/mL viable yeast cells during storage. Therefore, these five strains have potential to act as starters in the formulation of water kefir, which is an anti-hyperglycaemic beverage that can be used to manage type-2 diabetes mellitus.
Mushroom cultivation has been more popular recently in Malaysia. They are favoured due to their delicious flavour and low calorific value. Apart from that, they also contain high amount of protein and other essential nutrients. As recommended by food pyramid, people should take in more of their calories from whole grains-based foods than any other sources. Three selected carbohydrate based products namely rice-porridge (RP), paratha flat bread (PB) and conventional cake (CC) were formulated with dried Pleurotus sajor-caju (PSC) powder. All three products were analyzed for proximate analysis and sensory evaluation. Result shows the percentage of moisture, ash, fat and protein of RP increased in line with the levels of PSC powder used except for carbohydrate. Whereas for PB and CCs added with 2%, 4% and 6% PSC, the percentage of all nutrients were higher than control (0%) except for fat. Mushroombased RP had significantly higher value of odour attribute as compared to control, with RP added with 6% PSC powder received the highest score. Meanwhile, mushroom-based PB received better score on textural attribute compared the control. In CC, panels prefer the cake added with 4% PSC powder as they gave higher scores for softness and flavour attributes. In conclusion, addition of PSC powder to partially replace rice and wheat flour in RP, CC and PB enhance essential nutritional components and well accepted by consumers. Thus, PSC powder can be considered to be utilized in carbohydrate–based food products with the purpose of enhancing nutrient compositions without affecting its sensory acceptance.
Ascorbic acid or vitamin C is mostly found in natural products such as fruits and vegetables. High performance liquid chromatography (HPLC) method has been developed and validated to compare the ascorbic acid content in Benincasa hispida (Bh) fruit extract with three different extraction solvents; i) 3% metaphosphoric acid, ii) 3% citric acid and iii) distilled water. The compound has been detected and quantified by the use of HPLC coupled with UV-Vis detector. The amount of ascorbic acid detected in Bh fruit extract prepared with different extraction solvents; 3% metaphosphoric acid, 3% citric acid and distilled water were 13.18, 7.91 and 9.42 mg/100g respectively. Total run time was 6 min and the retention time was 2.60 min. Calibration curve was linear with the concentration range 1.00 – 16.00 μg/ml. Limits of detection was 0.24 μg/ml, limit of quantification was 0.81 μg/ml and recovery was 93.52%. The result showed ascorbic acid content is higher in Bh fruit extract with 3% metaphosphoric acid, followed by extract with distilled water and 3% citric acid. Thus, Bh is another novel fruit/ vegetable potentially used as food ingredient as it contains a good source of ascorbic acid that can be good for one’s health.
Discovery of novel dietary fibre (DF)-rich food ingredient is of great interest to meet the rising consumer demand for healthy food. Intake of DF-enriched food has been positively associated with a decreased risk of chronic diseases. Pleurotus sajor-caju (PSC), one of the prominent edible mushrooms widely cultivated in Malaysia, is useful for its culinary and medicinal purpose. This study aimed to determine the nutritional, physical, colour and sensorial properties of cinnamon biscuit formulated with PSC powder as partial replacement (0, 4, 8 and 12%) for wheat flour. Results indicated that elevated incorporation levels of PSC powder significantly (P
The present study was conducted to investigate the effects of brown rice (BR) powder addition on the proximate composition, total dietary fibre content and acceptability of some selected Malaysian traditional rice-based local kuih. Two types of kuih samples, namely Kuih Lompang (KL) and Kuih Talam Pandan (KTP) were prepared at the levels of either 0%, 10%, 20% or 30%. The kuih samples were analyzed for nutritional composition and sensory acceptance. There was significant increase in total dietary fibre content (from 2.64 g/100 g to 3.15 g/100 g) and protein content (from 2.36% to 2.51%) with the incorporation of 90% BR powder in the KL formulation. The moisture (from 36.79% to 36.83%), ash (from 1.11% to 1.21%) and fat (from 8.51% to 8.73%) content were not significantly affected for all percentages of BR powder addition. For KTP, the addition of BR powder at the level of 90% significantly increased the total dietary fibre (from 2.77 g/100 g to 3.45 g/100 g), fat (from 5.73% to 6.95%) and moisture (from 64.10% to 64.12%) content as compared to the control (0%). However, the protein content was not significantly affected (from 3.41% to 3.59%). On the other hand, there was no significant difference for all sensory attributes of KL formulated with 30-90% of BR powder as compared to the control (0%). The sensory score of KTP added with 30-90% BR powder received significantly lower sensory score compared to the control sample (0%) for appearance, colour, firmness, adhesiveness, chewiness, taste and overall acceptance attributes. In summary, sensory evaluation showed that all BR-incorporated KL were acceptable, while only 30% addition of BR powder in KTP was acceptable. Thus, BR powder is potentially used in improving the nutritional composition of KL. However, further study is needed to improve palatability aspect of KTP formulated with BR powder.
The influence of oyster mushroom (pleurotus sajor-caju, PSC) powder on the physical
properties of herbal seasoning (HS) was investigated. The pH, total solid, viscosity, rheology
and texture of semi solid HS containing different PSC powder level (0%, 20%, 40%, 60%,
8%, 100% w/w) of coconut milk powder were measured. The pH of the products were in the
range of 4.05 - 4.15. Rheological behavior was characterized by oscillatory rheometry. Stress
sweep, frequency sweep and steady stress experiments were conducted to study the behavior
of the products. The products showed non Newtonian characteristic or shear thinning. All
samples were G’> G’’ showed the gel like network. In addition, the back extrusion rig texture
analysis showed the correlation among the samples were also studied. Total substitution of PSC
powder (100% w/w) in the formulation resulted more viscous product and the combination
of the coconut milk powder and PSC powder showed the best spreadability and flow to the
product characteristics. No added PSC powder (0% w/w) showed the least viscous products
and the less moduli among the samples studied. The present study suggested the incorporation
of more than 40% PSC powder to replace coconut milk powder give better flowability and not
affect the viscosity of the products.
The intake of dietary fibre (DF) has been proven to lower the risk of chronic diseases, leading to the increasing demand for fibre-enriched bakery product. Banana is one of the most consumed fruits that exhibits rich sources of DF and provides excellent nutritional health benefits. However, overripe banana is discarded due to its low quality and appearance. Thus, the present work was aimed to determine the properties of chocolate cookies formulated with overripe banana pulp powder (OBPP) as partial replacement (0, 6, 8, and 10%) for wheat flour. Nutritional composition, physical properties, and sensory acceptability of the cookies were analysed using AOAC methods, texture profile analyser, and 7-point hedonic scaling method, respectively. Results showed that increased incorporation of OBPP significantly increased the nutritional values of chocolate cookies. Chocolate cookies formulated with 10% of OBPP recorded the highest total dietary fibre (8.21%) and ash (1.23%) contents. In texture profile analysis, the firmness of the chocolate cookies was recorded to increase slightly with increasing level of OBPP, although this was not significant. Sensory scores for the control (0%) and 6% OBPP-incorporated cookies were not significantly different for all the sensory attributes. However, the incorporation of 8% OBPP produced the highest scores in terms of aroma, flavour, and overall acceptance. In summary, the addition of 8% OBPP could be an effective way to produce nutritious and the most palatable chocolate cookies.
The aim of the present work was to develop legume-based kefir yogurts to replace conventional dairy yogurts that are not suitable to be consumed by vegetarians and consumers who have lactose intolerance and milk allergy. Soy and black bean milk were incubated at 15°C and 20°C for 24 h with 35 g of water kefir grains to produce kefir yogurt. The proximate composition, physico-chemical and microbiological characteristics of the yogurts were evaluated. At 20°C, soy and black bean milk produced kefir yogurts with significantly lower pH, total soluble solids, and sucrose concentration, indicating that fermentation process carried out at 20°C had higher efficiency than 15°C; meanwhile, black bean milk produced better kefir yogurts than soymilk. Black bean kefir yogurt which was fermented at 20°C had higher level of total plate count (2.05 × 107 CFU/mL), yeast and mould count (6.95 × 106 CFU/mL), and lactobacilli count (8.3
× 105 CFU/mL) as compared to other kefir yogurts. In general, 20°C produced kefir yogurt with better technological properties. Both soymilk and black bean milk were good alternative substrates for kefir yogurt production.
The effect of storage time on the quality of ultraviolet-irradiated and thermally pasteurised pineapple juice was evaluated. The juices were irradiated with ultraviolet light (UV-C) at wavelength 254 nm (53.42 mJ/cm2, 4.918 s), thermally pasteurised at 800C for 10 minutes and stored at 40C for 13 weeks. There were significant changes in the total soluble solids, pH, titratable acidity and turbidity of UV-irradiated juice during storage, whereas for the same quality attributes of thermally pasteurised juice remained stable throughout the storage time. There were no significant changes in total phenolics for both treatments throughout the storage period. Other quality parameters (ascorbic acid, colour L, hue angle and chroma) were significantly affected by the storage time. Regarding the microbiological analysis, the total plate counts and yeast and mould counts of the UV-irradiated juice increased gradually throughout the 13 weeks of storage while these parameters remained unchanged in the thermally pasteurised juice with almost no microorganism growth. UV-irradiated pineapple juice preserved better quality attributes (TSS, pH, titratable acidity, ascorbic acid, turbidity, total phenolic, L (lightness), hue angle and chroma) than the thermal pasteurised juice during the storage time. Hence, UV irradiation has great potential as an alternative technology to thermal pasteurisation in producing products of high nutritive values.
This work aimed to evaluate the effect of Atmospheric Cold Plasma (ACP) on the quality of mango flour noodles (NMF). ACP treatment of 5 minutes duration on the surface of the noodles strands were performed and evaluated during three days of storage by monitoring parameters related to colour, water activity, antioxidant activity and total phenolic content. The lightness value (L*) was higher for untreated samples (NMF (U)) than for treated samples (NMF (T)), while a greater increased in the redness (a*) and yellowness (b*) values were observed for the NMF (T). The changes in aw, antioxidant activity and total phenolic content (TPC) were negligible. However the NMF (T) showed significant different (p
Marine fungi are potential source of bioactive compounds as indicated by the increasing statistic
of research findings. However similar research in Malaysia is still lacking. Hence, this study
is undertaken to determine the antibacterial activity of four marine fungal isolates (PR1T4,
PP2L4, PR3T13 and PR5T4) from Pulau Redang and Pulau Payar Marine Parks, Malaysia
against Salmonella Typhi, Listeria monocytogenes, Staphylococcus aureus, and Escherichia
coli. Fungal isolates were first macroscopically and microscopically characterized and later
molecularly identified as Penicillium citrinum, Sacroladium strictum, Aspergillus sydowii and
Aspergillus sp. respectively. Solid and broth fermentation of fungi were carried out to produce
crude extracts and these extracts were screened for antibacterial activity. In general, solid
fermentation extracts (SFE) showed significantly higher antibacterial activity (p
The detection of tetrodotoxin (TTX) and saxitoxin (STX) in dried salted yellow puffer fish (Xenopterus naritus) eggs bought from Satok Market, Kuching, Sarawak was carried out by mouse bioassay method. The amount of TTX and STX detected in the samples ranged from 95.6-195.5 Mouse Unit (MU)/g and 1.72-3.58 MU/g respectively. The results indicate that the dried salted eggs samples were found to contain TTX 9-20 times above the regulatory limit for human consumption (10 MU/g). Although detected, the amount of STX in salted eggs extract was slightly below the accepted threshold limit (4 MU/g). The local public in Sarawak should be educated on the potential danger of consuming dried salted puffer fish eggs in addition to the current warnings on puffer fish.
‘Nasi kandar’ is defined as boiled white rice placed inside a wooden basket and carried on the shoulder using a pole. The rice is served with a variety of pairing dishes that are usually fried, curried or boiled. The tradition of carrying rice and pairing dishes on the shoulder began to die out in the 20th century as seller resorted to a more conventional way of selling ‘nasi kandar’. The ‘nasi kandar’ business in Malaysia has evolved from being sold as a street food into a multi-million dollar franchise business rivalling the fast food business. It is available 24 hours and enjoyed by Malaysians from all walks of life. This study aims to compare ‘nasi kandar’ eating pattern among patrons in the Klang Valley and Penang. Three hundred sets of questionnaires were answered and collected. Data from the questionnaires were analyzed in PASW 18.0 using descriptive statistics and chi-square test for association. Klang Valley patrons (72%) would consume ‘nasi kandar’ once a week. More than half (53.3%) of the patrons from Penang consumed ‘nasi kandar’ more than twice a week. A significant relationship exists between patron’s region and frequency of ‘nasi kandar’ consumption where p = <0.0001 and r = 0.277. A significant but moderate relationship between patron’s region and consumption of ‘nasi kandar’ during breakfast (p = <0.001, r = 0.250), lunch (p = <0.0001, r = 264) and dinner (p = <0.0001, r = 0.336). Patrons in Penang consumed ‘nasi kandar’ more often than their Klang Valley counterparts. Klang Valley patrons were more particular than Penang patrons when it comes to cleanliness, menu pricing, friendly service, air-condition and separate smoking area. Klang Valley patrons tend to consume more chicken based dishes while Penang patrons tend to consume more meat, fish, and squid based dishes.
Microbiological qualities of fresh goat milk collected from two selected, popular dairy farms in Penang Island, Malaysia were evaluated, as a measure of food safety. Milk samples were screened for total plate counts, yeast and mould counts, psychrotrophic counts, Staphylococcus aureus, presumptive Escherichia coli, Coliforms and Klebsiella pneumoniae, which were in the range of (mean values) 4.2- 4.5, 4.2- 4.6, 3.1- 4.3, 2.7- 3.2, < 2- 4.6, 2.2- 4.0 and 4.1- 4.8 log CFU/ml, respectively in the two farms. Milk samples were also screened for the presence of selected foodborne pathogens such as Listeria monocytogenes and Salmonella sp. Results
showed the presence of only Salmonella sp. (at 2.9 log CFU/ml) with the absence of Listeria monocytogenes. The outcome of this study assumes importance as the presence of microbial contaminants amounts indicates poor milk quality, which requires immediate consideration as it can pose serious health risk to consumers.
Protein-rich by-products from the canning industry, especially dark flesh of skipjack, have limited uses due to several factors such as darken color, susceptibility to oxidation and off flavour. Protein hydrolysates from skipjack dark flesh was produced with different type of industrial proteases (Alcalase®2.4L FG, Protamex®, Neutrase®1.5MG and Flavourzyme®500MG) for 60, 120, 180 and 240 min with level of proteases used of 0.5, 1, 1.5 and 2% per weight of raw material. The degree of hydrolysis and free tryptophan content of hydrolysate were investigated. The results shows longer time with higher concentration of enzyme has increased the degree of hydrolysis. Alcalase®2.4L FG had the highest degree of hydrolysis among all proteases followed by Protamex®, Flavourzyme®500MG and Neutrase® 1.5MG. All enzymes increase free tryptophan content linearly with the increament of protease enzyme level. The longer the hydrolysis time, the higher the content of free tryptophan produced.
The present work was carried out to formulate a pasta with oyster mushroom flour (OMF), and to evaluate the effect of its addition on the nutritional compositions, physicochemical properties, and sensorial acceptability of the formulated pasta. Four pasta formulations with different levels of OMF (0%, 5%, 10%, and 15%) were developed prior to the determination of the nutritional composition, physicochemical properties, and sensorial acceptability. Proximate components such as protein, ash, and fat increased with increasing OMF levels except for carbohydrates and energy, which were decreased with increasing OMF levels. Lightness (L*) and yellowness (b*) significantly decreased with increasing OMF levels, while redness (a*) increased with increasing OMF levels. The results showed that the optimum cooking time, elasticity, firmness, and shear work decreased with increasing OMF levels, while there were no significant differences in hardness or adhesiveness between the samples. In sensory evaluation, the pasta fortified with up to 10% OMF was generally well accepted by the sensory panellists. The addition of 10% OMF into the pasta resulted in an increase in its nutritional values, thus affecting its physicochemical properties, and improving some of its sensorial attributes.
The aim of this study were to find out the physicochemical characteristics of cholesterol-reduced egg yolk powder and its application in the production of mayonnaises. Cholesterol-reduced egg yolk powder (CREYP) were prepared from removal of cholesterol by formation of cholesterol:β-cyclodextrin inclusion complex. The physicochemical characteristics of CREYP and NEYP were foaming capacity (FC): 1.96%, 4%; foaming stability (FS): 96.48%, 94.55%; emulsion capacity (EC): 59.82%, 58.43% and emulsion stability (ES): 43.94%, 41.48% respectively. Whereas the viscosity of CREY, NEY and commercial mayonnaises were 8000, 4768 and 6747 cP respectively. The lightness (L*), redness (a*), yellowness (b*), saturation (C*) and hue angle (h°) values for CREYP and NEYP results showed significantly different (p>0.05) for all chroma values with CREYP showed higher L* and h° values but lower in a*, b* and C* values showing that the yellow colour of NEYP lessened. Commercial mayonnaise appeared to be lighter and less yellowish than CREY mayonnaises with L* and b* values of
commercial to CREY mayonnaises were L*: 78.34; 63.78% and b*: 8.29; 14.98% respectively. It can be concluded that CREYP can be used as replace to the NEYP and whole liquid egg yolk
with enhance nutritional values. The results obtained from this study will be very useful for producing CREYP.
The antioxidant capacity and total phenolic content of seeds of three types of pitaya namely Hylocereus polyrhizus, Hylocereus undatus and Selenicereus megalanthus and the fatty acid content of pitaya seeds oil were examined in this study. The ethanolic extracts of H. polyrhizus seeds showed significantly high (p