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Fulltext Comparative metabolomics of Phialemonium curvatum as an omnipotent fungus cultivated on crude palm oil versus glucose

Zamani AI, Barig S, Ibrahim S, Mohd Yusof H, Ibrahim J, Low JYS, et al.

Microb Cell Fact, 2020 Sep 09;19(1):179.
PMID: 32907579 DOI: 10.1186/s12934-020-01434-w

BACKGROUND: Sugars and triglycerides are common carbon sources for microorganisms. Nonetheless, a systematic comparative interpretation of metabolic changes upon vegetable oil or glucose as sole carbon source is still lacking. Selected fungi that can grow in acidic mineral salt media (MSM) with vegetable oil had been identified recently. Hence, this study aimed to investigate the overall metabolite changes of an omnipotent fungus and to reveal changes at central carbon metabolism corresponding to both carbon sources.
RESULTS: Targeted and non-targeted metabolomics for both polar and semi-polar metabolites of Phialemonium curvatum AWO2 (DSM 23903) cultivated in MSM with palm oil (MSM-P) or glucose (MSM-G) as carbon sources were obtained. Targeted metabolomics on central carbon metabolism of tricarboxylic acid (TCA) cycle and glyoxylate cycle were analysed using LC-MS/MS-TripleQ and GC-MS, while untargeted metabolite profiling was performed using LC-MS/MS-QTOF followed by multivariate analysis. Targeted metabolomics analysis showed that glyoxylate pathway and TCA cycle were recruited at central carbon metabolism for triglyceride and glucose catabolism, respectively. Significant differences in organic acids concentration of about 4- to 8-fold were observed for citric acid, succinic acid, malic acid, and oxaloacetic acid. Correlation of organic acids concentration and key enzymes involved in the central carbon metabolism was further determined by enzymatic assays. On the other hand, the untargeted profiling revealed seven metabolites undergoing significant changes between MSM-P and MSM-G cultures.
CONCLUSIONS: Overall, this study has provided insights on the understanding on the effect of triglycerides and sugar as carbon source in fungi global metabolic pathway, which might become important for future optimization of carbon flux engineering in fungi to improve organic acids production when vegetable oil is applied as the sole carbon source.

Matched MeSH terms: Lipids/biosynthesis
Fulltext Severe hypertriglyceridemia in a nondiabetic treated with low dose insulin infusion

Tong CV, Velaiutham S, Aziz NA, Lim SL, Khaw CH

Med J Malaysia, 2015 Aug;70(4):249-50.
PMID: 26358023

We report a case of a 54-year-old man with severe HTG which did not respond to conventional anti lipid therapies. He was treated with intravenous insulin and concurrent dextrose infusions which led to a dramatic reduction in serum triglyceride levels.

Matched MeSH terms: Lipids
Impact of various microalgal-bacterial populations on municipal wastewater bioremediation and its energy feasibility for lipid-based biofuel production

Leong WH, Azella Zaine SN, Ho YC, Uemura Y, Lam MK, Khoo KS, et al.

J Environ Manage, 2019 Nov 01;249:109384.
PMID: 31419674 DOI: 10.1016/j.jenvman.2019.109384

The microalgal-bacterial co-cultivation was adopted as an alternative in making microbial-based biofuel production to be more feasible in considering the economic and environmental prospects. Accordingly, the microalgal-bacterial symbiotic relationship was exploited to enhance the microbial biomass yield, while bioremediating the nitrogen-rich municipal wastewater. An optimized inoculation ratio of microalgae and activated sludge (AS:MA) was predetermined and further optimization was performed in terms of different increment ratios to enhance the bioremediation process. The nitrogen removal was found accelerating with the increase of the increment ratios of inoculated AS:MA, though all the increment ratios had recorded a near complete total nitrogen removal (94-95%). In light of treatment efficiency and lipid production, the increment ratio of 0.5 was hailed as the best microbial population size in accounting the total nitrogen removal efficiency of 94.45%, while not compromising the lipid production of 0.241 g/L. Moreover, the cultures in municipal wastewater had attained higher biomass and lipid productions of 1.42 g/L and 0.242 g/L, respectively, as compared with the synthetic wastewater which were only 1.12 g/L (biomass yield) and 0.175 g/L (lipid yield). This was possibly due to the presence of trace elements which had contributed to the increase of biomass yield; thus, higher lipid attainability from the microalgal-bacterial culture. This synergistic microalgal-bacterial approach had been proven to be effective in treating wastewater, while also producing useful biomass for eventual lipid production with comparable net energy ratio (NER) value of 0.27, obtained from the life-cycle analysis (LCA) studies. Thereby, contributing towards long-term sustainability and possible commercialization of microbial-based biofuel production.

Matched MeSH terms: Lipids
Effects of light intensity and nutrients on the lipid content of marine microalga (diatom) Amphiprora sp. for promising biodiesel production

Jayakumar S, Bhuyar P, Pugazhendhi A, Rahim MHA, Maniam GP, Govindan N

Sci Total Environ, 2021 May 10;768:145471.
PMID: 33736330 DOI: 10.1016/j.scitotenv.2021.145471

In this research investigation, three microalgal species were screened (Pleurosigma sp., Amphora sp., and Amphiprora sp.) for lipid content before choosing the potential microalgae for biodiesel production. It was found that the lipid content of Amphiprora sp. was 41.48 ± 0.18%, which was higher than the Pleurosigma sp. (27.3 ± 0.8%) and Amphora sp. (22.49 ± 0.21%). The diatom microalga, Amphiprora sp. was isolated and exposed to a controlled environment. Two different media were prepared, and the main research was on the SiO2-NP medium as the cell wall of diatom was made up of silica. Essential growth parameters were studied such as dry cell weight and chlorophyll a content. The results revealed that Amphiprora sp. cultured in the modified medium showed a higher biomass yield and growth rate in all the analyses. In Soxhlet extraction method, biodiesel yield of Amphiprora sp. in modified medium under 24 μmol m-2 s-1 of light intensity was 81.47 ± 1.59% when using 2% of catalyst amount with 1.5:1 volume ratio of methanol/oil in 3 h reaction time at 65 °C. Results reveled that Amphiprora sp. diatom has a higher yield of oil 52.94 ± 0.42% and can be efficiently optimized with further studies with modified nanomaterial culture medium. The present research revealed the series of experiments on microalgal lipid transesterification and in future investigation different types of nanomaterials should be used in culture medium to identify the lipid production in microalgal cells.

Matched MeSH terms: Lipids
Small interfering RNA for cancer treatment: overcoming hurdles in delivery

Charbe NB, Amnerkar ND, Ramesh B, Tambuwala MM, Bakshi HA, Aljabali AAA, et al.

Acta Pharm Sin B, 2020 Nov;10(11):2075-2109.
PMID: 33304780 DOI: 10.1016/j.apsb.2020.10.005

In many ways, cancer cells are different from healthy cells. A lot of tactical nano-based drug delivery systems are based on the difference between cancer and healthy cells. Currently, nanotechnology-based delivery systems are the most promising tool to deliver DNA-based products to cancer cells. This review aims to highlight the latest development in the lipids and polymeric nanocarrier for siRNA delivery to the cancer cells. It also provides the necessary information about siRNA development and its mechanism of action. Overall, this review gives us a clear picture of lipid and polymer-based drug delivery systems, which in the future could form the base to translate the basic siRNA biology into siRNA-based cancer therapies.

Matched MeSH terms: Lipids
Spinach leaf and chloroplast lipid: A natural rheology modifier for chocolate?

Mohamad NJ, Gray D, Wolf B

Food Res Int, 2020 07;133:109193.
PMID: 32466904 DOI: 10.1016/j.foodres.2020.109193

In this study the possibility of replacing current surfactants in chocolate formulations with natural lipids extracted from spinach leaf (SPLIP) or spinach chloroplast (CH.SPLIP) was evaluated. SPLIP and CH.SPLIP were extracted with chloroform/methanol following enzyme deactivation with hot isopropanol. Results showed a higher extraction yield for SPLIP while glycolipids were more concentrated in CH.SPLIP. Sugar/oil suspensions with dispersed volume fractions of 0.28, 0.33 and 0.37 containing 0.1% to 0.7% (w/w) surfactant (SPLIP, CH.SPLIP, lecithin and PGPR as commercial references) based on oil phase were prepared and analyzed in shear rheology. Apparent viscosity at 40 s-1 was significantly lower for the natural surfactants compared to lecithin at 0.5-0.7% (w/w) addition. With regard to yield stress, taken as the shear stress at 5 s-1, both natural surfactants showed comparable performance to PGPR at 0.3% to 0.7% addition. As SPLIP and CH.SPLIP behaved similar (p > 0.05), SPLIP, due to higher extraction yield, would be the preferred choice for application in chocolate matrices.

Matched MeSH terms: Lipids
The occurrence of a furanoid fatty acid inHevea brasiliensis latex

Hasma H, Subramaniam A

Lipids, 1978 Dec;13(12):905-7.
PMID: 27520427 DOI: 10.1007/BF02533847

Methyl esters from the triglyceride fraction of the neutral lipids of natural rubber latex were found by gas liquid chromatography to contain about 90% of a furanoid acid. Spectroscopic analysis identified the acid as 10,13-epoxy-11-methyloctadeca-10,12-dienoic acid.

Matched MeSH terms: Lipids
Food waste compost as an organic nutrient source for the cultivation of Chlorella vulgaris

Chew KW, Chia SR, Show PL, Ling TC, Arya SS, Chang JS

Bioresour Technol, 2018 Nov;267:356-362.
PMID: 30029182 DOI: 10.1016/j.biortech.2018.07.069

The present study investigates the prospective of substituting inorganic medium with organic food waste compost medium as a nutrient supplement for the cultivation of Chlorella vulgaris FSP-E. Various percentages of compost mixtures were replaced in the inorganic medium to compare the algal growth and biochemical composition. The use of 25% compost mixture combination was found to yield higher biomass concentration (11.1%) and better lipid (10.1%) and protein (2.0%) content compared with microalgae cultivation in fully inorganic medium. These results exhibited the potential of combining the inorganic medium with organic food waste compost medium as an effective way to reduce the cultivation cost of microalgae and to increase the biochemical content in the cultivated microalgae.

Matched MeSH terms: Lipids
Composition and sources of sterols in Pulau Tinggi, Johor, Malaysia

Masni Mohd Ali, Norfariza Humrawali, Mohd Talib Latif, Mohamad Pauzi Zakaria

Sains Malaysiana, 2011;40.

This study explores the role of sterols as lipid biomarkers to indicate their input which originates from various sources in the marine environment. Sterols and their ratios were investigated in sediments taken from sixteen sampling stations at Pulau Tinggi, Johor in order to assess the sources of organic matter. The compounds extracted from the sediments were quantified using a gas chromatography-mass spectrometry (GC-MS). The distributions of sterols indicated that organic matter at all sampling stations originated from a mixture of marine source and terrestrial origins at different proportions. A total of eleven sterols were quantified, with the major compounds being phytosterols (44% of total sterols), cholesterol (11%), brassicasterol (11%) and fecal sterols (12%).

Matched MeSH terms: Lipids
Novel Nanostructured Lipid Carriers Co-Loaded with Mesalamine and Curcumin: Formulation, Optimization and In Vitro Evaluation

Awasthi A, Kumar B, Gulati M, Vishwas S, Corrie L, Kaur J, et al.

Pharm Res, 2022 Nov;39(11):2817-2829.
PMID: 36195824 DOI: 10.1007/s11095-022-03401-z

PURPOSE: The aim of current study is to formulate, optimize and characterize the developed formulation of Mesalamine-Curcumin Nanostructured Lipid Carriers (Mes-Cur NLCs).
METHODS: It was formulated using high pressure homogenization followed by probe sonication and formulation variables were optimized using Central Composite Design. The particle size (PS), zeta potential (ZP), entrapment efficiency (EE), drug release, cytotoxicity on NIH 3T3 fibroblasts cells and HaCaT keratinocytes cells and efficacy on RAW264.7 cells for optimized formulation was determined.
RESULTS: The PS, ZP and EE were found to be 85.26 nm, -23.7 ± 7.45 mV, 99.2 ± 2.62 % (Mes) and 84 ± 1.51 % (Cur), respectively. The good correlation between predicted and obtained value indicated suitability and reproducibility of experimental design. NLCs showed spherical shape as confirmed by TEM. In vitro drug release profile of prepared formulation showed that Mes exhibited 100 % release at 48 h, whereas Cur exhibited 82.23 ± 2.97% release at 120 h. Both the drugs exhibited sustained release upon incorporation into the NLCs. The absence of any significant cell death during MTT assay performed on NIH 3T3 fibroblasts cells and HaCaT keratinocytes cells indicated that NLCs' were safe for use. Furthermore, significant reduction in nitric oxide level during anti-inflammatory evaluation of formulation on RAW264.7 cells showed excellent potential for the formulation to treat inflammation. The formulation was found stable as no significant difference between the PS, ZP and EE of the fresh and aged NLCs was observed.
CONCLUSION: The outcomes of study deciphered successful formulation of Mes-Cur NLCs.

Matched MeSH terms: Lipids
Fatty acid profile from immobilised Chlorella vulgaris cells in different matrices

Abu Sepian NR, Mat Yasin NH, Zainol N, Rushan NH, Ahmad AL

Environ Technol, 2019 Apr;40(9):1110-1117.
PMID: 29161985 DOI: 10.1080/09593330.2017.1408691

The immobilisation of Chlorella vulgaris 211/11B entrapped in combinations of natural matrices to simplify the harvesting process was demonstrated in this study. Three combinations of matrices composed of calcium alginate (CA) and sodium alginate (SA), sodium carboxymethyl cellulose (CMC) and SA, and mixed matrices (SA, CA, and CMC) were investigated. The number of cells grown for each immobilised matrix to microalgae volume ratios (0.2:1-1:1) were explored and compared with using SA solely as a control. The optimum volume ratios obtained were 1:1 for SA, 0.3:1 for CA and SA, 1:1 for CMC and SA, and 0.3:1 for mixed matrices. The immobilised microalgae of mixed matrices exhibited the highest number of cells with 1.72 × 109 cells/mL at day 10 and 30.43% of oil extraction yield followed by CA and SA (24.29%), CMC and SA (13.00%), and SA (6.71%). Combining SA, CA, and CMC had formed a suitable structure which improved the growth of C. vulgaris and increased the lipid production compared to the immobilisation using single matrix. Besides, the fatty acids profile of the oil extracted indicates a high potential for biodiesel production.

Matched MeSH terms: Lipids
Enhanced single cell oil production by mixed culture of Chlorella pyrenoidosa and Rhodotorula glutinis using cassava bagasse hydrolysate as carbon source

Liu L, Chen J, Lim PE, Wei D

Bioresour Technol, 2018 May;255:140-148.
PMID: 29414159 DOI: 10.1016/j.biortech.2018.01.114

The single cell oil (SCO) production by the mono and mixed culture of microalgae Chlorella pyrenoidosa and red yeast Rhodotorula glutinis was investigated using non-detoxified cassava bagasse hydrolysate (CBH) as carbon source. The results suggested that the two strains were able to tolerate and even degrade some byproducts presented in the CBH, and the mixed culture approach enhanced the degradation of certain byproducts. Biomass (20.37 ± 0.38 g/L) and lipid yield (10.42 ± 1.21 g/L) of the mixed culture achieved in the batch culture were significantly higher than that of the mono-cultures (p 

Matched MeSH terms: Lipids
Fulltext Targeting Membrane Lipid a Potential Cancer Cure?

Tan LT, Chan KG, Pusparajah P, Lee WL, Chuah LH, Khan TM, et al.

Front Pharmacol, 2017;8:12.
PMID: 28167913 DOI: 10.3389/fphar.2017.00012

Cancer mortality and morbidity is projected to increase significantly over the next few decades. Current chemotherapeutic strategies have significant limitations, and there is great interest in seeking novel therapies which are capable of specifically targeting cancer cells. Given that fundamental differences exist between the cellular membranes of healthy cells and tumor cells, novel therapies based on targeting membrane lipids in cancer cells is a promising approach that deserves attention in the field of anticancer drug development. Phosphatidylethanolamine (PE), a lipid membrane component which exists only in the inner leaflet of cell membrane under normal circumstances, has increased surface representation on the outer membrane of tumor cells with disrupted membrane asymmetry. PE thus represents a potential chemotherapeutic target as the higher exposure of PE on the membrane surface of cancer cells. This feature as well as a high degree of expression of PE on endothelial cells in tumor vasculature, makes PE an attractive molecular target for future cancer interventions. There have already been several small molecules and membrane-active peptides identified which bind specifically to the PE molecules on the cancer cell membrane, subsequently inducing membrane disruption leading to cell lysis. This approach opens up a new front in the battle against cancer, and is of particular interest as it may be a strategy that may be prove effective against tumors that respond poorly to current chemotherapeutic agents. We aim to highlight the evidence suggesting that PE is a strong candidate to be explored as a potential molecular target for membrane targeted novel anticancer therapy.

Matched MeSH terms: Membrane Lipids
Upcycling of food waste generated from the fresh market by utilising black soldier fly larvae: Influence on growth, bioconversion, and nutritional composition

Taufek NM, Mohamad Zulkifli NFN, Hamizah AN

J Environ Manage, 2024 Jan 01;349:119467.
PMID: 37976636 DOI: 10.1016/j.jenvman.2023.119467

Innovative solutions are needed to limit environmental effect and optimise resource use as food waste generation rises worldwide. This study investigates the potential of upcycling food waste from fresh markets using Black Soldier Fly (Hermetia illucens) larvae (BSFL) as a sustainable approach. This study explored four fresh market food waste substrates for BSFL bioconversion: discarded fish waste (FI), slaughtered chicken waste (CHI), vegetable waste (VEG), and a 1:1:1 combination of all three (MIX). Soybean curd residue (SCR) was treated as the control substrate. The effects on larval growth, nutritional content, and waste bioconversion rates were examined. The larvae growth rate was strongly impacted by waste type, with BSF-fed CHI and MIX gaining 18.0 and 16.7 mg/d, respectively, followed by BSF-fed with SCR (12.2 mg/d), FI (8.9 mg/d) and VEG (7.6 mg/d). The waste type did not substantially alter BSFL length. The survival rate of the BSFL fed with the food waste studied ranges from 95 to 98.47%, with SCR being the highest. Our findings indicated that BSFL can effectively convert a variety of fresh market food waste into valuable biomass. CHI waste produced the highest larval biomass and bioconversion rate followed by MIX, SCR, FI and VEG. The different waste stream has a major influence on BSFL biomass nutrition. BSFL nutritional composition is independent of the substrate's nutritional content, indicating no direct correlation between substrate and BSFL biomass nutritional composition. SCR waste produced the highest protein content of BSFL (50.49%), followed by VEG (32.61%), MIX (32.57%), FI (31.03%) and CHI (29.06%). SCR waste also produced BSFL biomass with lowest lipid content (26.55%) compared to other waste which resulted into BSFL with lipid levels ranging from 42.92% to 53.72%. BSFL-fed with SCR is the most suitable to be used as an alternative animal's feed based on the protein and lipid levels, while defatting procedure is necessary for the other waste-fed BSFL to render it suitability as animal feed alternatives. Based on bioconversion rate, BSFL growth, and lipid content, the MIX and CHI waste might be viable substrates for future research.

Matched MeSH terms: Lipids
Enhancing the various solvent extraction method via microwave irradiation for extraction of lipids from marine microalgae in biodiesel production

Teo CL, Idris A

Bioresour Technol, 2014 Nov;171:477-81.
PMID: 25201293 DOI: 10.1016/j.biortech.2014.08.024

The types of microalgae strains and the method used in lipid extraction have become crucial factors which influence the productivity of crude oil. In this paper, Nannochloropsis sp. and Tetraselmis sp. were chosen as the strains and four different methods were used to extract the lipids: Hara and Radin, Folch, Chen and Bligh and Dyer. These methods were performed by using conventional heating and microwave irradiation methods. Results revealed that highest lipid yield from the different species was obtained using different extraction methods; both under microwave irradiation. The lipid yield for Tetraselmis sp. and Nannochloropsis sp. was highest when Hara and Radin (8.19%), and Folch (8.47%) methods were used respectively under microwave irradiation. The lipids extracted were then transesterified to biodiesel and the quality of the biodiesel was analyzed using the gas chromatography.

Matched MeSH terms: Lipids/analysis; Lipids/isolation & purification*
Lipid constituents of the edible mushroom, Pleurotus giganteus demonstrate anti-Candida activity

Phan CW, Lee GS, Macreadie IG, Malek SN, Pamela D, Sabaratnam V

Nat Prod Commun, 2013 Dec;8(12):1763-5.
PMID: 24555294

Different solvent extracts of Pleurotus giganteus fruiting bodies were tested for antifungal activities against Candida species responsible for human infections. The lipids extracted from the ethyl acetate fraction significantly inhibited the growth of all the Candida species tested. Analysis by GC/MS revealed lipid components such as fatty acids, fatty acid methyl esters, ergosterol, and ergosterol derivatives. The sample with high amounts of fatty acid methyl esters was the most effective antifungal agent. The samples were not cytotoxic to a mammalian cell line, mouse embryonic fibroblasts BALB/c 3T3 clone A31. To our knowledge, this is the first report of antifungal activity of the lipid components of Pleurotus giganteus against Candida species.

Matched MeSH terms: Lipids/toxicity; Lipids/chemistry*
An improved method for the preparations of nanostructured lipid carriers containing heat-sensitive bioactives

Hung LC, Basri M, Tejo BA, Ismail R, Nang HL, Abu Hassan H, et al.

Colloids Surf B Biointerfaces, 2011 Oct 1;87(1):180-6.
PMID: 21652183 DOI: 10.1016/j.colsurfb.2011.05.019

Heat-sensitive bioactive compounds such as β-carotene and tocols, are widely used in the pharmaceutical and cosmetic fields. Their chemical stability in delivery systems is one of the major concerns in the production of nanostructured lipid carriers (NLCs). A previously established high-temperature high-pressure homogenisation technique involved in the preparation of NLCs can cause degradation of heat-sensitive compounds. Therefore, a novel preparation process needs to be developed to minimise the degradation of heat-sensitive active compounds during the preparation of NLCs. In this work, modified methods A and B were designed to minimise the degradation of β-carotene and tocols during the production of NLCs. These methods improved the chemical stability of heat-sensitive bioactive compounds (β-carotene and tocols) significantly compared to the previously established method. The physical stability of the formulation was maintained throughout study duration.

Matched MeSH terms: Lipids/chemical synthesis*; Lipids/chemistry
Lowering of lipid composition in aorta of guinea pigs by Curcuma domestica

Ahmad-Raus RR, Abdul-Latif ES, Mohammad JJ

BMC Complement Altern Med, 2001;1:6.
PMID: 11495637

A short-term study was carried out using guinea pigs to determine the effects of Curcuma domestica on lipid composition in the serum and aorta.

Matched MeSH terms: Lipids/analysis*; Lipids/blood
Interactive effects of dietary palm oil concentration and water temperature on lipid digestibility in rainbow trout, Oncorhynchus mykiss

Ng WK, Campbell PJ, Dick JR, Bell JG

Lipids, 2003 Oct;38(10):1031-8.
PMID: 14669967

An experiment was conducted to evaluate the interactive effects of dietary crude palm oil (CPO) concentration and water temperature on lipid and FA digestibility in rainbow trout. Four isolipidic diets with 0, 5, 10, or 20% (w/w) CPO, at the expense of fish oil, were formulated and fed to groups of trout maintained at water temperatures of 7, 10, or 15 degrees C. The apparent digestibility (AD) of the FA, measured using yttrium oxide as an inert marker, decreased with increasing chain length and increased with increasing unsaturation within each temperature regimen irrespective of CPO level fed to the fish. PUFA of the n-3 series were preferentially absorbed compared to n-6 PUFA in all diet and temperature treatments. Except for a few minor FA, a significant (P < 0.05) interaction between diet and temperature effects on FA digestibility was found. Increasing dietary levels of CPO lead to significant reductions in the AD of saturates and, to a lesser extent, also of the other FA. Lowering water temperature reduced total saturated FA digestibility in trout regardless of CPO level. Based on the lipid class composition of trout feces, this reduction in AD of saturates was due in part to the increasing resistance of dietary TAG to digestion. Increasing CPO level and decreasing water temperature significantly increased TAG content in trout fecal lipids, with saturates constituting more than 60% of the FA composition. Total monoene and PUFA digestibilities were not significantly affected by water temperature in fish fed up to 10% CPO in their diet. The potential impact of reduced lipid and FA digestibility in cold-water fish fed diets supplemented with high levels of CPO on fish growth performance requires further research.

Matched MeSH terms: Lipids/analysis; Lipids/classification
Biotechnology of oil palm: strategies towards manipulation of lipid content and composition

Parveez GK, Rasid OA, Masani MY, Sambanthamurthi R

Plant Cell Rep, 2015 Apr;34(4):533-43.
PMID: 25480400 DOI: 10.1007/s00299-014-1722-4

Oil palm is a major economic crop for Malaysia. The major challenges faced by the industry are labor shortage, availability of arable land and unstable commodity price. This has caused the industry to diversify its applications into higher value products besides increasing its yield. While conventional breeding has its limitations, biotechnology was identified as one of the tools for overcoming the above challenges. Research on biotechnology of oil palm began more than two decades ago leveraging a multidisciplinary approach involving biochemical studies, gene and promoter isolation, transformation vector construction and finally genetic transformation to produce the targeted products. The main target of oil palm biotechnology research is to increase oleic acid in the mesocarp. Other targets are stearic acid, palmitoleic acid, ricinoleic acid, lycopene (carotenoid) and biodegradable plastics. Significant achievements were reported for the biochemical studies, isolation of useful oil palm genes and characterization of important promoters. A large number of transformation constructs for various targeted products were successfully produced using the isolated oil palm genes and promoters. Finally transformation of these constructs into oil palm embryogenic calli was carried out while the regeneration of transgenic oil palm harboring the useful genes is in progress.

Matched MeSH terms: Lipids/analysis*; Lipids/biosynthesis

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