MyMedR

Synthesis, evaluation, molecular docking, and molecular dynamics studies of novel N-(4-[pyridin-2-yloxy]benzyl)arylamine derivatives as potential antitubercular agents

Verma R, Boshoff HIM, Arora K, Bairy I, Tiwari M, Varadaraj BG, et al.

Drug Dev Res, 2020 05;81(3):315-328.

PMID: 31782209 DOI: 10.1002/ddr.21623

A new series of novel triclosan (2,4,4'-trichloro-2'-hydroxydiphenylether) analogues were designed, synthesized, and screened for their in vitro antimycobacterial and antibacterial activities. Most of the compounds showed significant activity against Mycobacterium tuberculosis H37Rv strain with minimum inhibitory concentration (MIC) values in 20-40 μM range in GAST/Fe medium when compared with triclosan (43 μM) in the first week of assay, and after additional incubation, seven compounds, that is, 2a, 2c, 2g, 2h, 2i, 2j, and 2m, exhibited MIC values at the concentration of 20-40 μM. The compounds also showed more significant activity against Bacillus subtilis and Staphylococcus aureus. The synthesized compounds showed druggable properties, and the predicted ADME (absorption, distribution, metabolism, and excretion) properties were within the acceptable limits. The in silico studies predicted better interactions of compounds with target protein residues and a higher dock score in comparison with triclosan. Molecular dynamics simulation study of the most active compound 2i was performed in order to further explore the stability of the protein-ligand complex and the protein-ligand interaction in detail.

Matched MeSH terms: Staphylococcal Infections

Fulltext A typical presentation of infective endocarditis

Loong YY, Fadzli A, Lim PH, Rakhi G

Malaysian Journal of Medicine and Health Sciences, 2005;1(1):67-69.

We describe a patient who had fever and bilateral cortical blindness and was later found to have leaking intracerebral mycotic aneurysm secondary to Staphylococcus aureus endocarditis. He had a vegetation at the .tricuspid valve with evidence of systemic embolisation, however, chest radiograph did not reveal any evidence of pulmonary emboli.
Keywords: Endocarditis, cortical blindness, mycotic aneurysm, Staphylococcus

Matched MeSH terms: Staphylococcal Infections

Interaksi gal quercus infectoria dengan vankomisin terhadap staphylococcus aureus yang kurang kerentanan terhadap vankomisin

Norza Wani Jaffar, Dayang Fredalina Basri, Noraziah M. Zin

Sains Malaysiana, 2011;40:1237-1241.

Staphylococcus aureus tahan Methicillin (MRSA) adalah patogen yang mudah tersebar dan cenderung untuk menjadi multi-rintangan terhadap hampir semua terapi antibiotik yang terdapat di pasaran kini. Gal daripada Quercus infectoria atau lebih dikenali sebagai manjakani di Malaysia telah diketahui mempunyai aktiviti anti-bakteria. Tujuan kajian ini adalah untuk mengkaji kesan kombinasi ekstrak gal daripada Q. infectoria dengan vankomisin terhadap lima strain MRSA dan dua strain Vancomycin-intermediate Staphylococcus aureus (VISA). Proses pengekstrakan dilakukan menggunakan pelarut aseton, metanol dan akues. Teknik mikro pencairan bersiri kaldu dijalankan menggunakan plat telaga 96 bagi menentukan nilai Minimum Inhibitory Concentration (MIC). Kesan interaksi di antara gal Q. infectoria dengan vankomisin dikaji menggunakan teknik checkerboard bagi mendapatkan nilai indek Fractional Inhibitory Concentration (FIC). Hasil kombinasi ekstrak gal dan vankomisin menunjukkan terdapat aktiviti yang sinergi pada ekstrak aseton terhadap MRSA ATCC 43300 (0.3124), Sa7 (0.3750) dan VISA U9495 (0.3750), manakala ekstrak akues terhadap MRSA Sa7 (0.4167) dan VISA U9495 (0.3958) dan N316 (0.5000). Sebaliknya kombinasi metanol dan vankomisin menunjukkan kesan yang sinergi terhadap ATCC 49476 (0.3750) sahaja manakala aditif terhadap enam lagi strain MRSA yang diuji. Kesan interaksi ini menunjukkan potensi dan kepentingannya dalam pembangunan dan modifikasi agen anti-MRSA.

Matched MeSH terms: Staphylococcal Infections

Synthesis, characterization and antibacterial activity of mono-, di- and tri-tosylate of glycerol

Yusrabbil Amiyati Yusof, Azhar Ariffin

Sains Malaysiana, 2016;45:621-625.

Glycerol is a valuable co-product from oleochemical industry such as from fatty acid and biodiesel production. By having three hydroxyl groups in its molecule, glycerol can undergo chemical modifications that lead to many possible applications. This paper reports the tosylation process of glycerol with para-toluenesulfonyl chloride (p-TsCl). Reaction of glycerol with p-TsCl in the presence of a base produced mono-, di- and tri-tosylate of glycerol even though the reaction was carried out at the mole ratio of 1.2:1.0 of glycerol to p-TsCl. The compounds were successfully isolated and characterized. Mono-, di- and tri-tosylate of glycerol exhibited inhibitory activity against Staphylococcus aureus (gram positive bacteria) and Pseudomonas aeruginosa (gram negative bacteria).

Matched MeSH terms: Staphylococcal Infections

Fulltext Microbiological quality evaluation of fried rice sold at food premises in Kuantan City, Pahang

Abu Bakar A, Abdul Rafa AA, Abdullah Sani A

International Journal of Allied Health Sciences, 2018;2(1):285-291.

MyJurnal

Food contamination is a crucial health problem as it could result in food-borne illness. This research aimed to evaluate the microbiological quality of ready-to-eat (RTE) fried rice dishes sold at different type of food premises in Kuantan city, Pahang. Total Plate Count (TPC), Staphylococcus aureus, Bacillus cereus and Aeromonas spp. bacteria were used as microbiological contamination indicators. About 52 samples were collected stratified randomly from four types of food premises (restaurant, cafeteria, food stall and night market) where about 13 samples were respectively collected from each type of the food premises. The results showed that TPC had medium mean count (6.30x105±1.47x105 cfu/g), S. aureus and B. cereus had high mean counts (7.70x104±2.22x105 cfu/g and 3.85x105±1.67x106 cfu/g respectively), while Aeromonas spp. had medium mean count (7.13x104±2.42x105 cfu/g). The mean counts of TPC in the samples collected from cafeteria were highest compare to other food premises.

Matched MeSH terms: Staphylococcal Infections

Fulltext Identification of Escherichia spp. strains in street-vended beverages and associated preparation surfaces using 16S rRNA analysis

Chong, S. Y., Rao, P. V., Soon, J. M.

International Food Research Journal, 2017;24(4):1811-1818.

MyJurnal

Street-vended beverages are commonly prepared by mechanical extraction of the pulp, usually
unpasteurised and requires multiple handling steps. Foodborne pathogens transmitted via skin of
street vendors or via faecal-oral route may contaminate the preparation surfaces and beverages.
The aim of this study is to identify Escherichia spp. strains of street-vended beverages and their
associated preparation surfaces using 16s rRNA analysis. The hygienic practice of vendors was represented by Staphylococcus spp. analysis and Staphylococcus aureus is not detected in beverages and associated preparation surfaces. A total of 80 samples (18 beverages, 15 swab
samples and 47 direct film samples) were collected followed by enumeration of microbial load.
Polymerase Chain Reaction (PCR) amplification and 16S ribosomal ribonucleic acid (rRNA)
sequencing were carried out. Results of 16S rRNA sequence analysis indicated that three gram-negative isolates were identified as Escherichia coli RM9387 (Accession no. CP009104.1),
Escherichia coli c164 (Accession no. JQ781646.1) and Escherichia fergusonii E10 (Accession no. KJ626264.1) with similarity value of 99% respectively.

Matched MeSH terms: Staphylococcal Infections

Distribution of sasX, qacA/B and mupA genes and determination of genetic relatedness of methicillin-resistant Staphylococcus aureus among clinical isolates and nasal swab samples from the same patients in a hospital in Malaysia

Nurhafiza NNBA, Siti Asma H, Azian H, Foo PC, Yasmin KMI, Chan YY

Singapore Med J, 2020 12 02.

PMID: 33264563 DOI: 10.11622/smedj.2020166

INTRODUCTION: This study determined the distribution of sasX, qacA/B and mupA genes from methicillin-resistant Staphylococcus aureus (MRSA) isolated from clinical samples and nasal swab samples of the same patients and analysed their genetic relatedness.

METHODS: Polymerase chain reaction (PCR) was used to detect the presence of sasX, qacA/B and mupA genes from 47 paired MRSA isolates. A paired isolate was defined as one nasal swab (colonising) isolate and clinical isolate that caused infection in the same patient. 22 selected paired isolates were subjected to multilocus sequence typing (MLST). The genetic relatedness among the isolates and association between the putative genes with epidemic sequence types (STs) were investigated.

RESULTS: 7 (14.9%, n = 14) paired isolates were positive for the sasX gene. qacA/B genes were positive in 7.4% (n = 7) of the isolates, from three paired isolates and one clinical isolate whose paired colonising isolate was negative. The paired sample of three patients were positive for both genes. The mupA gene was not detected in all the isolates. MLST revealed two epidemic STs, ST22 and ST239, and a novel ST4649. sasX and qacA/B genes were found in ST239 in 29.5% (n = 13) and 13.6% (n = 6) of cases, respectively. Gene co-existence occurred in 13.6% (n = 6) of MRSA ST239 and 2.3% (n = 1) of MRSA ST4649.

CONCLUSION: sasX and qacA/B genes were present in the MRSA isolates, while the mupA gene was undetected. ST22 and ST239 were the major MRSA clones. The circulating MRSA genotypes conferred different virulence and resistance determinants in our healthcare settings.

Matched MeSH terms: Staphylococcal Infections

Fulltext Prevalence of ST9 methicillin-resistant Staphylococcus aureus among pigs and pig handlers in Malaysia

Neela V, Mohd Zafrul A, Mariana NS, van Belkum A, Liew YK, Rad EG

J Clin Microbiol, 2009 Dec;47(12):4138-40.

PMID: 19812280 DOI: 10.1128/JCM.01363-09

Methicillin-resistant Staphylococcus aureus (MRSA) of sequence type 398 (ST398) has frequently been detected in pigs and pig handlers. However, in Malaysia, sampling 360 pigs and 90 pig handlers from 30 farms identified novel ST9-spa type t4358-staphylococcal cassette chromosome mec type V MRSA strains that were found to transiently colonize more than 1% of pigs and 5.5% of pig handlers.

Matched MeSH terms: Staphylococcal Infections/microbiology; Staphylococcal Infections/epidemiology*; Staphylococcal Infections/veterinary

Fulltext Staphylococcus aureus carriage in selected kindergartens in Klang Valley

Mohamed NA, Ramli S, Amin NN, Sulaiman WS, Isahak I, Jamaluddin TZ, et al.

Med J Malaysia, 2016 04;71(2):62-5.

PMID: 27326943 MyJurnal

INTRODUCTION: Nasal colonisation of S. aureus in healthy children was 18% to 30%. One to three percent of them were colonised by Methicillin-resistant Staphlycoccus aureus (MRSA). Although MRSA infection has become increasingly reported, population-based S. aureus and MRSA colonisation estimates are lacking. The main objective of this study was to determine the prevalence of S. aureus carriage among children.

METHODS: Nasal samples for S. aureus culture were obtained from 250 children from three kindergartens in the Klang Valley, after consent was obtained from the children and their parents. Swabs were transported in Stuart medium, and inoculated on mannitol-salt agar within four hours of collection. Identification and disk diffusion test were done according to guidelines. Polymerase chain reaction was done on MRSA isolates for the presence of mecA and lukS/FPV genes.

RESULTS: Overall prevalence of S. aureus and MRSA carriage were 19.2% (48/250) and 1.6% (4/250) respectively. mecA gene was present in all isolates, 50% isolates carried Panton-Valentine leucocidin (PVL) gene. Sccmec type I was found in 2 isolates and the remaining isolates has Sccmec type V.

CONCLUSION: The prevalence of S. aureus and MRSA carriage were similar to other studies. However, risk of contracting severe infection might be higher due to presence of PVL gene in half of the MRSA isolates.

Matched MeSH terms: Staphylococcal Infections/diagnosis*

Fulltext High prevalence of methicillin-resistant Staphylococcus aureus (MRSA) on doctors' neckties

Koh KC, Husni S, Tan JE, Tan CW, Kunaseelan S, Nuriah S, et al.

Med J Malaysia, 2009 Sep;64(3):233-5.

PMID: 20527275 MyJurnal

We set out to investigate whether neckties worn by doctors are more likely to be contaminated with Methicillin resistant Staphylococcus aureus (MRSA) compared to neckties worn by preclinical medical undergraduates who have never been exposed to a hospital environment. We discovered that more than half (52%) of neckties worn by doctors were contaminated with Staphylococcus and out of these, 62% of them were identified as MRSA. In contrast, none of the student's ties were contaminated with MRSA. Due to the high prevalence of staphylococcus detected on doctors' neckties, we recommend that health care workers do not wear neckties.

Matched MeSH terms: Staphylococcal Infections/transmission*

Fulltext Doctor's attire and patient safety

Abu Bakar S

Med J Malaysia, 2009 Sep;64(3):185-6.

PMID: 20527264 MyJurnal

Koh and others have reported (in this issue of the MJM) the high prevalence of Methicillin resistant Staphylococcus aureus (MRSA) on doctors’ neckties. As they have pointed out, this is nothing new, and like other studies with similar findings. They also point out that patient’s confidence and satisfaction are not affected by doctor’s not wearing neckties. They also referred to the British Department of Health decision to ban the use of neckties, long sleeve shirts and jewellery, and the Scottish government’s intention to ban the wearing of white coats, including neckties, to stop the spread of infections. They support the call by The Malaysian Medical Association to avoid the use of neckties.

Matched MeSH terms: Staphylococcal Infections/transmission*

Fulltext Vancomycin treatment failure in a vancomycin susceptible methicillin-resistant Staphylococcus aureus (MRSA) infected patient

Norazah A, Salbiah N, Nurizzat M, Santhana R

Med J Malaysia, 2009 Jun;64(2):166-7.

PMID: 20058580 MyJurnal

A 64-year old patient, who had bacteraemia, did not respond to vancomycin despite the MRSA isolate being sensitive to the antibiotic at MIC 2 microg/mL. Electron microscopy of the MRSA isolate showed thickening of the cell wall, which was not observed in MRSA with lower vancomycin MIC.

Matched MeSH terms: Staphylococcal Infections/drug therapy*

Florid peripheral stigmata of staphylococcus aureus infective endocarditis

Yew KL, Razali F

Acute Card Care, 2015;17(2):32.

PMID: 26458028 DOI: 10.3109/17482941.2015.1066822

Matched MeSH terms: Staphylococcal Infections/pathology*

Fulltext Mycotic bronchial artery aneurysmal rupture in the early stage of lung abscess: A case report

Mohd Alkaf AL, Syed Rasul SH, Abdul Rahman I

Med J Malaysia, 2016 04;71(2):96-7.

PMID: 27326956 MyJurnal

Symptomatic bronchial artery aneurysm warrants urgent intervention. It has a known association with pulmonary infection caused by Staphylococcus aureus. We hereby report an elderly lady with a ruptured left superior bronchial artery mycotic aneurysm. She was in the early stages of treatment for a left lung abscess. She had multiple episodes of haemoptysis following which she underwent a left lower lobectomy. Presentation of lung abscess with a concurrent ruptured mycotic aneurysm warrants early surgical intervention and can be curative as seen in this case.

Matched MeSH terms: Staphylococcal Infections/complications*

Orbital abscess due to acute ethmoiditis in a neonate

Reddy SC, Sharma HS, Mazidah AS, Darnal HK, Mahayidin M

Int J Pediatr Otorhinolaryngol, 1999 Jun 15;49(1):81-6.

PMID: 10428409

Orbital complications due to ethmoiditis are not uncommon in children. However, they are very rare in infants. A case of orbital abscess due to acute ethmoiditis in a 10 days old boy is reported. Causative microorganisms isolated from the operated specimen were Staphylococcus aureus and aspergillosis. Successful outcome was achieved following antimicrobial therapy, external ethmoidectomy, and surgical drainage of the abscess. The aetiopathogenesis and management of this clinical entity is discussed, with a brief review of the literature.

Matched MeSH terms: Staphylococcal Infections/complications*

Pyomyositis revisited

Kong NC, Asmah J, Lim VK, Ong PH, Adam PA

Ann Acad Med Singap, 1996 Jul;25(4):609-11.

PMID: 8893941

Pyomyositis, purportedly a common tropical infection affecting mainly healthy adults and children, appears to be most uncommon in this region. We report a case of pyomyositis caused by a Methicillin-resistant Staphylococcus aureus (MRSA) in a previously healthy army officer. This case serves to illustrate the difficulty in recognising this disease entity, which is why many cases may have been missed. With the increasing incidence of MRSA nosocomial infections, the emergence of MRSA in a hitherto community-acquired infection poses a major concern especially since intravenous drug abuse and acquired immune deficiency syndrome (AIDS) are on the rise in our country. We hope to inculcate greater awareness of this infection.

Matched MeSH terms: Staphylococcal Infections*

Fulltext Acute stridor in childhood; retropharyngeal abscess

Yeo TC

Med J Malaysia, 1988 Mar;43(1):65-73.

PMID: 3244324

Thirty-eight patients with acute stridor were admitted to the Paediatric Unit of the Alor Setar General Hospital over a three-year period (1984 - 1986). The causes are discussed and retropharyngeal abscess is highlighted as it is often initially overlooked. The clinical presentation and the problems in the diagnosis of the latter are discussedwith reference to the three cases seen.
Key words: Acute stridor in childhood, retropharyngeal abscess

Matched MeSH terms: Staphylococcal Infections/complications*

Fulltext Dermatitis cruris pustulosa et atrophicans: case reports

Ting HC

Med J Malaysia, 1984 Mar;39(1):82-4.

PMID: 6513846

The presence of dermatitis cruris pustulosa et atrophicans in three local patients is documented and the condition briefly discussed.

Matched MeSH terms: Staphylococcal Infections/pathology*

Fulltext Pattern of neonatal septicemia in a Malaysian maternity hospital

Boo NY, Wong YH, Lim VK

Med J Malaysia, 1989 Sep;44(3):189-93.

PMID: 2626133

Over a 12 months period, out of 25,411 livebirths, 155 neonates (6.1 per 1000 livebirths) had proven septicemia by blood culture. The mortality rate was 26.5%. Septicemia was more common among the very low birthweight and preterm neonates of gestation of 30 weeks or less. 45.8% of the septicemia occurred during the first 48 hours of life. Staphylococcus epidermidis was the most common causative organism. However, mortality was highest among neonates who acquired multiresistant nosocomial infection during the later part of neonatal life.

Matched MeSH terms: Staphylococcal Infections/epidemiology

Fulltext Prevalence of Staphylococcus aureus carriage by young Malaysian footballers during indoor training

William JL, Radu S, Aziz SA, Rahim RA, Cheah YK, Liwan A, et al.

Br J Sports Med, 2004 Feb;38(1):12-4.

PMID: 14751938

BACKGROUND: Research has shown that athletes are carriers of Staphylococcus aureus during physical activity.
OBJECTIVE: To estimate the mean total plate count of S aureus carried by footballers before and after training at an indoor venue.
METHODS: Forty Malay and 20 Indian students volunteered to participate. There was also a control group consisting of 40 Malay and 20 Indian students who were not active. The experimental group were active footballers who had played at school or club level. The subjects were healthy and free of skin infection. The experiment was divided into three sessions, with 20 subjects present at each. At each session, the subjects trained for one hour. Swabs were taken from the skin, nose, and ear before and after training. For the control group, swabs were taken only once from the skin, nose, and ear. The swabs were subjected to biochemical tests and then streaked and cultured aerobically in Baird Parker agar plates for 24 hours at 37 degrees C. Black colonies with a clear zone were presumed to be S aureus, and the mean total plate count of the colonies was estimated. Gram staining, catalase, coagulase slide, coagulase tube, acetoin production, o-nitrophenyl beta-D-galactopyranoside (ONPG), and mannitol fermentation tests were used to confirm the colonies as S aureus. A haemolysin test was conducted with human blood to confirm haemolytic activity.
RESULTS: All subjects in the experimental group were carrying S aureus both before and after training. The estimated mean total counts of colonies from the skin, ear, and nose for the Malays before training were 33, 71, and 312 respectively. Counts after training were 21, 44, and 452 respectively. The results for the Indians were 72, 80, and 309 respectively before training and 55, 200, and 466 respectively after training. The positive results for Gram staining, catalase, coagulase slide, coagulase tube, acetoin production, ONPG, and mannitol fermentation tests were 100%, 96%, 95%, 95%, 93%, 93%, and 90% respectively. All subjects in the control group were also carrying S aureus.
CONCLUSIONS: All of the players were carriers of S aureus during training. The decrease in total count from the skin for both races may be due to lysozyme activity lysing the bacterial cells. Contamination of the environment with these bacteria may have increased the estimated total plate count in the nose. The experimental group face a higher risk of infection because of lower immunity during training and higher rate of injuries compared with the control group.

Matched MeSH terms: Staphylococcal Infections/microbiology*

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