Displaying publications 61 - 80 of 1574 in total

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  1. Mazumder SK, Das SK, Bakar Y, Ghaffar MA
    J Zhejiang Univ Sci B, 2016 Aug;17(8):580-90.
    PMID: 27487803 DOI: 10.1631/jzus.B1500251
    In this study we aimed to analyze the effects of water temperature and diet on the length-weight relationship and condition of juvenile Malabar blood snapper Lutjanus malabaricus over a 30-d experimental period. The experiment was conducted in the laboratory using a flow-through-sea-water system. The fish were subjected to four different temperatures (22, 26, 30, and 34 °C) and two diets (commercial pellet and natural shrimp). Fish were fed twice daily. L. malabaricus exhibited negative allometric growth (b<3) at the beginning of the experiment (Day 0) at all temperatures and both diets except for 22 °C fed with shrimp, which showed isometric growth (b=3). Conversely, at the end of the experiment (Day 30) fish showed isometric growth (b=3) at 30 °C fed with the pellet diet, indicating that the shape of the fish did not change with increasing weight and length, and a positive allometric growth (b>3) at 30 °C fed with shrimp diet, which indicated that fish weight increases faster than their length. The rest of the temperatures represented negative allometric growth (b<3) on both diet, meaning that fish became lighter with increasing size. The condition factors in the initial and final measurements were greater than 1, indicating the state of health of the fish, except for those fed on a pellet diet at 34 °C. However, the best condition was obtained at 30 °C on both diets. Nevertheless, diets did not have a significant effect on growth and condition of juvenile L. malabaricus. The data obtained from this study suggested culturing L. malabaricus at 30 °C and feeding on the pellet or shrimp diet, which will optimize the overall production and condition of this commercially important fish species.
    Matched MeSH terms: Perciformes/growth & development*
  2. Dinh TD, Ambak MA, Hassan A, Phuong NT
    Pak J Biol Sci, 2007 Oct 01;10(19):3284-94.
    PMID: 19090143
    This study describe the reproductive biological characteristics and population parameters of the goby, Pseudapocryptes elongatus (Cuvier, 1816), in the coastal mud flat areas of the Mekong Delta, Vietnam. A total of 1058 specimens was collected from January 2004 to June 2005 and results showed that the breeding season occurred with two spawning peaks in July and October. Length at first maturity (L(m)) was 15.4 and 16.3 cm for females and males, respectively. The batch fecundity estimates ranged from 2,652 to 29,406 hydrated oocytes per ovary in the fish ranging from 12.8 to 22.4 cm TL. Length frequency data of the goby ranging from 9.0 to 24.0 cm TL were analyzed using the FiSAT II software. The von Bertalanffy growth parameters were determined as L8 = 25.9 cm, K = 0.66 year(-1) and t(o) = - 0.26 year(-1). The longevity (t(max)) of the goby was estimated to be 4.55 years. There were two recruitment peaks with very different magnitudes and the means of these two peaks were separated by an interval of 5 months. Length at first capture (L(c)) was 10.05 cm, the instantaneous fishing mortality rate (F = 1.38 year(-1)) and natural mortality rate (M = 1.46 year(-1)) accounted for 49 and 51% of the total mortality (Z = 2.84 year(-1)), respectively. Relative yield-per-recruit and biomass-per-recruit analyses gave E(max) = 0.65, E0.1 = 0.55 and E0.5 = 0.33. Results show that the fish stock is subjected to growth overexploitation.
    Matched MeSH terms: Fishes/growth & development
  3. Johan I, Maznah WO, Mashhor M, Abu Hena MK, Amin SM
    Pak J Biol Sci, 2012 Jul 01;15(13):647-52.
    PMID: 24218935
    Investigation on copepod communities in Perai river estuary was conducted from November 2005 to May 2006. Five stations were established for monthly sampling and were located from the river mouth to the upper reaches of the river. Copepod samples were collected from vertical tows using a standard zooplankton net. The Perai river estuary was slightly stratified and salinity decreases significantly from the mouth of the river towards the upper reaches of the river. A total of 28 species of copepods were recorded and comprised of 14 families, Paracalanidae, Oithonidae, Corycaeidae, Acartiidae, Calanidae, Centropagidae, Eucalanidae, Pontellidae, Pseudodiaptomidae, Tortanidae, Ectinosomatidae, Euterpinidae, Clausidiidae and Cyclopidae. A total of 10 species showed high positive affiliation towards salinity (R > 0.60), Acartia spinicauda, Euterpina acutifrons, Microsetella norvegica, Oithona nana, Oithona simplex, Paracalanus crassirostris, Paracalanus elegans, Paracalanus parvus, Pseudodiaptomus sp. and Hemicyclops sp. The copepod species Pseudodiaptomus dauglishi were negatively affiliated towards salinity (R = -0.71). The copepod assemblages classified into two distinct groups according to salinity regimes, euryhaline-polyhaline group (25 marine affiliated species) and oligohaline-mesohaline group (3 freshwater affiliated species).
    Matched MeSH terms: Copepoda/growth & development*
  4. Tee HS, Lee CY
    J Econ Entomol, 2017 12 05;110(6):2504-2511.
    PMID: 29029091 DOI: 10.1093/jee/tox241
    Many female parasitoid wasps optimize host selection to balance the benefits of high-quality hosts and the costs of predator- or hyperparasitoid-induced mortality risks to maximize their fitness. Cannibalism exists in many insect species and affects survival of parasitoid larvae developing in or on parasitized hosts. However, little is known about how parasitoid wasps resolve the fitness consequence of host cannibalism-induced mortality risk during host selection. We examined the effect of oothecal age on cannibalism in the American cockroach Periplaneta americana (L.) (Dictyoptera: Blattidae) and its effect on host age selection and fitness of its oothecal parasitoid Evania appendigaster (L.) (Hymenoptera: Evaniidae). P. americana differentially cannibalized 1-d-old (30‒60%) versus 10- to 40-d-old oothecae (<9%). However, parasitoid females did not avoid but still preferred to parasitize 1-d-old (45%) over 10- to 40-d-old oothecae (1.6‒20%). The parasitism rate was greater and the handling time was shorter on 1-d-old compared to older oothecae. For parasitoid progeny emerging from different-aged oothecae, regression analysis showed that development time increased and body size (measured as hind tibia length) and longevity decreased with oothecal age. These results demonstrate that reduced parasitoid progeny survival due to host cannibalism did not change the parasitoid's oviposition preference for newly laid oothecae, and that E. appendigaster females traded progeny survival for fitness gains for themselves and their progeny.
    Matched MeSH terms: Larva/growth & development; Nymph/growth & development; Ovum/growth & development; Periplaneta/growth & development*; Wasps/growth & development
  5. Peng TL, Sajap AS, Jeen LH, Lee SH, Lum WC
    Pak J Biol Sci, 2013 Dec 01;16(23):1786-90.
    PMID: 24506049
    One species of lace bug Cochlochila bullita Stål (Heteroptera: Tingidae) was found heavily infested Orthosiphon aristatus Blume Miq., an important medicinal plant in Malaysia. A morphological re-description of C. bullita was done in order to facilitate the identification of this oligophagous insect pest. Five variables, body length and width, antenna length, tibia length and head width were measured from 15 samples from each stage. Among these variables, body length and width were used to construct the ratio for species identification; while body lengths with the other three variables were used to distinguish the nymphs from each developmental stage. The measurements of four traits except the antenna length showed significant differences between the development stages. And thus suggest the body width, tibia length and head width were suitable parameters used to distinguish the nymphal stages. However, the result on the growth factor showed only the sizes of the head followed a more constant growth rate with growth ratios (1.21-1.39) lie between the Dyar's ratio. Body length and width ratio for the adult female and male was 1.51 +/- 0.00 and 1.59 +/- 0.01, respectively. These data are pertinent for identifying developmental stages and to distinguish the species of the lace bug.
    Matched MeSH terms: Head/growth & development; Nymph/growth & development; Tibia/growth & development; Heteroptera/growth & development*; Arthropod Antennae/growth & development
  6. Zuha RM, Omar B
    Parasitol Res, 2014 Jun;113(6):2285-94.
    PMID: 24728523 DOI: 10.1007/s00436-014-3883-z
    Cosmopolitan scuttle fly, Megaselia scalaris (Loew) (Diptera: Phoridae) is one of the commonest forensic species recorded colonizing human corpse indoors and in concealed environment. The occurrence of this species in such environments provides a higher evidential value to assist estimation of postmortem interval (PMI) compared to other forensically important dipterans. However, developmental and size data of M. scalaris are still lacking and they are derived from a limited range of thermal values. The objective of this study is to develop the growth model of M. scalaris by emphasizing the size range of larvae and puparia at different constant temperatures. This species was reared in six replicates at eight varying constant temperatures ranging from 23 to 36 °C and cow's liver was provided as food source. Larvae and puparia were sampled at set time intervals and measured by their length and weight. Because interpretation of forensic entomological evidence is subject to application of different techniques, development of M. scalaris is expressed herein by using developmental table, length/morphological stage diagrams and linear/nonlinear estimation methods. From the findings, it is very important to highlight that sexual dimorphism of M. scalaris during post feeding larva and pupa stage could be observed based on size and developmental periods. Mean length and weight ratios of male to female puparia are approximately 0.8 and 0.3-0.5, respectively, indicating sexual dimorphism of this species. Developmental period in female are 4.0-11.4 h (post feeding larval stage), 3.7-24.0 h (pupal stage), and 3.0-20.1 h (total developmental period) longer in male. Due to this dimorphism, PMI estimation using M. scalaris post feeding larva or puparium specimens must be carried out carefully by to avoid inaccuracy and misinterpretation.
    Matched MeSH terms: Diptera/growth & development*; Larva/growth & development; Ovum/growth & development; Pupa/growth & development
  7. Abu Tahir N, Ahmad AH
    J Med Entomol, 2013 Sep;50(5):999-1002.
    PMID: 24180104
    Effects of laterite cover soil with different characteristics on survival of buried eggs, third instar larvae, and pupae of Musca domestica (L.) were studied experimentally. Soil treatments were loose dry soil, loose wet soil, compacted dry soil, and compacted wet soil (CWS). Eggs, third instar larvae, and pupae were buried under 30 cm of the different soil treatments and placed under field conditions until adults emerged. Rearing medium was provided for eggs and larvae, and control treatments of all stages were unburied immatures placed on soil surface. Egg and pupal survival to adult were significantly affected by the cover soil treatments, but third instars were more resilient. Wet soil treatments (loose wet soil and CWS) resulted in significantly reduced pupal survival, but increased survival of eggs. However, CWS significantly reduced adult emergence from buried eggs. Though emergence of house flies buried as eggs was significantly reduced, some were able to hatch and emerging first instar larvae developed to pupation. Although cover soil does not completely prevent fly emergence, it did limit development and emergence of buried house flies.
    Matched MeSH terms: Houseflies/growth & development; Larva/growth & development; Ovum/growth & development; Pupa/growth & development
  8. Zuharah WF, Fadzly N, Lester PJ
    J Med Entomol, 2013 Sep;50(5):1014-24.
    PMID: 24180106
    The presence of predators can have dramatic consequences on prey communities, not only by the direct effects of consumption but also through sublethal effects. We investigated the survival rate and subsequent life history of the mosquito Culex pervigilans Bergroth under the influence of its major predator, the backswimmer Anisops wakefieldi White. We established a field experiment with various treatments: 1) control without predators, 2) free-roaming A. wakefieldi (with one, three, or nine A. wakefieldi per container), 3) caged A. wakefieldi (empty cage without predators, with one, three, or nine A. wakefieldi in each cage, and 4) A. wakefieldi cues (with cues concentrations of one, three, or nine A. wakefieldi). Cx. pervigilans eggs were then taken from these four experimental treatments and reared in two different laboratory conditions: 1) in clean water without any traces of predators, or 2) in water with the same treatments as in field. The survival rate of Cx. pervigilans was significantly reduced by the presence of predators or their cues. Even after a brief exposure to waters containing predators or residual cues, the subsequent progeny and the ontogeny of the remaining survivors were still affected. The percentage of eggs that hatched, and the resulting mosquito population, was influenced by the presence of predators or their cues. Our results suggest that sublethal effects may be carried by surviving individuals primarily through the effects of stress, perhaps by epigenetic mechanisms. We may expect to observe similar plasticity in species or populations with high temporal or spatial variability in predation.
    Matched MeSH terms: Culex/growth & development*; Larva/growth & development; Ovum/growth & development; Pupa/growth & development
  9. Bong LJ, Neoh KB, Jaal Z, Lee CY
    J Med Entomol, 2013 Sep;50(5):1003-13.
    PMID: 24180105
    The effects of four temperatures (15, 23.5, 28, and 35 degrees C) on the biological characteristics of the rove beetle Paederus fuscipes Curtis were studied, and its cuticular permeability also was measured. Specimens successfully developed to adulthood at each temperature tested, but development time of each preadult stage significantly decreased with increasing temperature. Both egg and L1 stages required at least 80 degree days above a threshold of approximately 10 degrees C to develop to the subsequent stage. The lengthy development time and high survival rate of preadults at 15 degrees C suggests that P. fuscipes can survive in a harsh environment during cold weather by hibernating, and this ability could allow preadults to succeed ecologically in temperate countries. However, adult longevity was short, and no fecundity was recorded at 15 degrees C. At 28 degrees C, P. fuscipes exhibited a high survival rate of adults, which had a longer life span and high fecundity; thus, the population had the highest intrinsic rate of increase (0.0788 +/- 0.0051 d(-1)) and the shortest mean generation time (48.57 +/- 1.43 d) at 28 degrees C. At this temperature, the population might reach a size that could facilitate invasion into residential areas. However, in the absence of a hygric environment, P. fuscipes was unable to survive despite favorable temperature. Unlike in adults and pupae, high cuticular permeability values were found in the larval stages. This indicates that larvae are highly susceptible to desiccation, and it explains why the distribution of P. fuscipes is restricted to moist habitats.
    Matched MeSH terms: Beetles/growth & development; Larva/growth & development; Ovum/growth & development; Pupa/growth & development
  10. Takaoka H, Srisuka W, Saeung A, Otsuka Y, Choochote W
    Zootaxa, 2013;3694:280-8.
    PMID: 26312290
    Simulium (Nevermannia) khunklangense sp. nov. is described from females, males, pupae and larvae collected in Doi Inthanon National Park, Chiang Mai, Thailand. This new species is placed in the vernum species-group of the subgenus Nevermannia and is similar to S. (N.) chomthongense Takaoka & Srisuka described from Doi Inthanon National Park, Thailand, but is distinguished in the male by the number of enlarged upper-eye facets and the relative width of the hind basitarsus against the hind tibia and femur, and in the pupa by the short common basal stalk of the gill and the cocoon with an anterodorsal bulge or a short anterodorsal projection. Taxonomic notes are provided to separate this new species from five other known species of the vernum species-group, which share an accessory sclerite on the larval abdomen, a rare characteristics in this species-group.
    Matched MeSH terms: Animal Structures/growth & development; Larva/growth & development; Pupa/growth & development; Simuliidae/growth & development
  11. Silahuddin SA, Latif B, Kurahashi H, Walter DE, Heo CC
    J Med Entomol, 2015 Jan;52(1):9-23.
    PMID: 26336275 DOI: 10.1093/jme/tju001
    The stages of decomposition and the faunal succession on rabbit carcasses in three different habitats, namely jungle, rural, and highland areas, were studied. Three New Zealand White rabbit (Oryctolagus cuniculus) carcasses weighing ∼2 kg were sampled daily until the decomposition process was completed. Representative specimens of adult flies, larvae, pupa, and mites were collected from the carcasses and processed in the laboratory. There were differences in decomposition rate and faunal succession between the carcasses. The fastest rate of decomposition was recorded in rural area, and the slowest rate of decomposition was recorded in highland area. The carcasses exhibited the same pattern of colonization by adult flies, but the dominant species of larvae and adult flies on each carcass in specific habitats were different. The primary species of flies recorded in jungle were Chrysomya megacephala F., Chrysomya rufifacies (Macquart), Chrysomya chani Kurahashi, Chrysomya villenuevi Patton, Chrysomya nigripes Aubertin, Chrysomya pinguis (Walker), Hemipyrellia ligurriens (Wiedemann), Hemipyrellia tagaliana (Bigot), Hypopyiopsis fumipennis (Walker), Hypopygiopsis violacea (Macquart), and Hydrotaea spinigera Stein represented by both adults and larvae. Musca domestica L., Atherigona sp., Lioproctia pattoni (Senior-White), Lioproctia saprianovae Pape & Bänziger, and Seniorwhitea princeps (Wiedemann) were represented by adults only. The biodiversity of flies in the rural area were C. megacephala, C. rufifacies, H. ligurriens, Fannia canicularis L., Hydrotaea chalcogaster (Wiedemann), and Hyd. spinigera represented by both adults and larvae, meanwhile M. domestica, Atherigona sp., Boettcherisca peregrina (Robineau-Desvoidy), Parasarcophaga taenionota Wiedemann, Parasarcophaga scopariiformis Senior-White, and S. princeps were represented by adults only. The species of flies collected in the highland area were Lucilia porphyrina (Walker), C. megacephala, C. rufifacies, C. villenuevi, C. pinguis, H. ligurriens, Hyd. spinigera, Hyd. chalcogaster, F. canicularis, and Boettcherisca highlandica Kurahashi & Tan represented by both adults and larvae, whereas C. nigripes, Chrysomya thanomthini Kurahashi & Tumrasvin, M. domestica, Atherigona sp., Parasarcophaga albiceps Meigen, P. taenionota, Sepsidae, Phoridae, and Millichidae were represented by adults only. Faunal succession followed the sequence of dominant flies, i.e., Calliphoridae, Sarcophagidae, Muscidae, Sepsidae, and lastly Stratiomyidae for jungle, or Sepsidae for rural and highland studies. Mites, from suborders Mesostigmata, Prostigmata, Astigmatina, and Oribatida, were also recovered throughout decomposition, which could be used for future implementation in forensic investigations. The data obtained from this study could provide more accurate indicators for local forensic scientists in solving criminal cases especially on the determination of time and primary location of death.
    Matched MeSH terms: Acari/growth & development; Diptera/growth & development; Larva/growth & development; Pupa/growth & development
  12. Chew SY, Cheah YK, Seow HF, Sandai D, Than LT
    Anaerobe, 2015 Aug;34:132-8.
    PMID: 26028405 DOI: 10.1016/j.anaerobe.2015.05.009
    A conspicuous new concept of pathogens living as the microbial societies in the human host rather than free planktonic cells has raised considerable concerns among scientists and clinicians. Fungal biofilms are communities of cells that possess distinct characteristic such as increased resistance to the immune defence and antimycotic agents in comparison to their planktonic cells counterpart. Therefore, inhibition of the biofilm may represent a new paradigm for antifungal development. In this study, we aim to evaluate the in vitro modulation of vulvovaginal candidiasis (VVC)-causing Candida glabrata biofilms using probiotic lactobacilli strains. Probiotic Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 were shown to have completely inhibited C. glabrata biofilms and the results were corroborated by scanning electron microscopy (SEM), which revealed scanty structures of the mixed biofilms of C. glabrata and probiotic lactobacilli strains. In addition, biofilm-related C. glabrata genes EPA6 and YAK1 were downregulated in response to the probiotic lactobacilli challenges. The present study suggested that probiotic L. rhamnosus GR-1 and L. reuteri RC-14 strains inhibited C. glabrata biofilm by partially impeding the adherence of yeast cells and the effect might be contributed by the secretory compounds produced by these probiotic lactobacilli strains. Further investigations are required to examine and identify the biofilm inhibitory compounds and the mechanism of probiotic actions of these lactobacilli strains.
    Matched MeSH terms: Biofilms/growth & development*; Candida glabrata/growth & development; Lactobacillus reuteri/growth & development*; Lactobacillus rhamnosus/growth & development*
  13. Yazid SNE, Jinap S, Ismail SI, Magan N, Samsudin NIP
    Compr Rev Food Sci Food Saf, 2020 03;19(2):643-669.
    PMID: 33325175 DOI: 10.1111/1541-4337.12541
    In this review, we present the current information on development and applications of biological control against phytopathogenic organisms as well as mycotoxigenic fungi in Malaysia as part of the integrated pest management (IPM) programs in a collective effort to achieve food security. Although the biological control of phytopathogenic organisms of economically important crops is well established and widely practiced in Malaysia with considerable success, the same cannot be said for mycotoxigenic fungi. This is surprising because the year round hot and humid Malaysian tropical climate is very conducive for the colonization of mycotoxigenic fungi and the potential contamination with mycotoxins. This suggests that less focus has been made on the control of mycotoxigenic species in the genera Aspergillus, Fusarium, and Penicillium in Malaysia, despite the food security and health implications of exposure to the mycotoxins produced by these species. At present, there is limited research in Malaysia related to biological control of the key mycotoxins, especially aflatoxins, Fusarium-related mycotoxins, and ochratoxin A, in key food and feed chains. The expected threats of climate change, its impacts on both plant physiology and the proliferation of mycotoxigenic fungi, and the contamination of food and feed commodities with mycotoxins, including the discovery of masked mycotoxins, will pose significant new global challenges that will impact on mycotoxin management strategies in food and feed crops worldwide. Future research, especially in Malaysia, should urgently focus on these challenges to develop IPM strategies that include biological control for minimizing mycotoxins in economically important food and feed chains for the benefit of ensuring food safety and food security under climate change scenarios.
    Matched MeSH terms: Aspergillus/growth & development; Fungi/growth & development*; Fusarium/growth & development; Penicillium/growth & development
  14. Joe LK, Hoa KE, Kong OC
    PMID: 5167566
    Matched MeSH terms: Echinostoma/growth & development; Fasciola/growth & development; Schistosoma/growth & development; Trematoda/growth & development*
  15. Wingsanoi A, Siri N, McNeil JN
    J Econ Entomol, 2013 Aug;106(4):1648-52.
    PMID: 24020277
    The Malaysian fruit fly, Bactrocera latifrons (Hendel), is a pest of peppers (Capsicum spp.) in Thailand. A field trial was undertaken to determine whether five commonly used cultivars of C. annuum, with marked differences in morphology and pungency, varied in their susceptibility to infestation by B. latifrons. Experiments carried out in both the dry and rainy seasons showed temporal differences in the number of fruits per cultivar, but there was no effect of variety or season on the proportion of fruits attacked or the number of pupae obtained per infested fruit However, the number of dead larvae per infested fruit was significantly higher, and the percent of pupae giving rise to adults was lower for the larger sweet pepper than other cultivars tested.
    Matched MeSH terms: Capsicum/growth & development*; Larva/growth & development; Pupa/growth & development; Tephritidae/growth & development
  16. Ismail MF, Siraj SS, Daud SK, Harmin SA
    Gen Comp Endocrinol, 2011 Jan 1;170(1):125-30.
    PMID: 20888822 DOI: 10.1016/j.ygcen.2010.09.021
    Annual gonad hormonal profile of wild, matured mahseer (29 males and 23 female) averaging in weight between 0.95±0.26 and 1.19±0.23 kg for males and females, respectively, were investigated from November 2007 to November 2008 using enzyme-linked immunosorbent assay (ELISA) technique. Blood was collected from caudal vein, monthly and plasma separation by centrifugation was done to measure reproductive hormones: 17β-estradiol (E(2)), testosterone (T), and 11-keto-testosterone (11KT). Gonads were sampled for histology processing to observe their maturity. Highest T level in females and males was recorded at 0.22±0.016 and 0.88±0.014 ng/ml, respectively. The 11KT showed several peaks and the highest value was noted at 0.7±0.018 ng/ml in November 2008. The female E(2) initially was at 1.48±0.16 ng/ml and significantly increased (P<0.05) to 1.53±0.39 ng/ml in November 2008. Ovaries were laden with oocytes in several stages in all the samples while testes gonad showed a high level of spermatids throughout the year. Changes in plasma level of the gonadal hormones were correlated with the ovarian and testes maturities. In conclusion, the study suggests that mahseer can be categorized as asynchronized and multiple spawner. The information gathered is important for appropriate breeding and conservation programs of the Malaysian mahseer.
    Matched MeSH terms: Cyprinidae/growth & development; Gonads/growth & development*; Ovary/growth & development; Testis/growth & development
  17. Govender NT, Mahmood M, Seman IA, Mui-Yun W
    Pol J Microbiol, 2016 Aug 26;65(3):383-388.
    PMID: 29334072 DOI: 10.5604/17331331.1215619
    Ganoderma boninense, a phytopathogenic white rot fungus had sought minimal genetic characterizations despite huge biotechnological potentials. Thus, efficient collection of fruiting body, basidiospore and protoplast of G. boninense is described. Matured basidiocarp raised under the glasshouse conditions yielded a total of 8.3 × 104 basidiospores/ml using the low speed centrifugation technique. Mycelium aged 3-day-old treated under an incubation period of 3 h in lysing enzyme from Trichoderma harzianum (10 mg/ml) suspended in osmotic stabilizer (0.6 M potassium chloride and 20 mM dipotassium phosphate buffer) yielded the highest number of viable protoplasts (8.9 × 106 single colonies) among all possible combinations tested (regeneration media, age of mycelium, osmotic stabilizer, digestive enzyme and incubation period).
    Matched MeSH terms: Spores, Fungal/growth & development; Mycelium/growth & development; Ganoderma/growth & development*; Fruiting Bodies, Fungal/growth & development*
  18. Md Setamam N, Jaafar Sidik N, Abdul Rahman Z, Che Mohd Zain CR
    BMC Res Notes, 2014 Jun 30;7:414.
    PMID: 24981787 DOI: 10.1186/1756-0500-7-414
    BACKGROUND: Capsicum annuum and Capsicum frutescens, also known as "chilies", belong to the Solanaceae family and have tremendous beneficial properties. The application of hairy root culture may become an alternative method for future development of these species by adding value, such as by increasing secondary metabolites and improving genetic and biochemical stability compared with normal Capsicum plants. Therefore, in this research, different types of explants of both species were infected with various Agrobacterium rhizogenes strains to provide more information about the morphology and induction efficiency of hairy roots. After 2 weeks of in vitro seed germination, young seedling explants were cut into three segments; the cotyledon, hypocotyl, and radical. Then, the explants were co-cultured with four isolated A. rhizogenes strains in Murashige & Skoog culture media (MS) containing decreasing carbenicillin disodium concentrations for one month.

    RESULTS: In this experiment, thick and short hairy roots were induced at all induction sites of C. annuum while thin, elongated hairy roots appeared mostly at wound sites of C. frutescens. Overall, the hairy root induction percentages of C. frutescens were higher compared with C. annuum. Hairy root initiation was observed earliest using radicles (1st week), followed by cotyledons (2nd week), and hypocotyls (3rd week). Cotyledon explants of both species had the highest induction frequency with all strains compared with the other explants types. Strains ATCC 13333 and ATCC 15834 were the most favourable for C. frutescens while ATCC 43056 and ATCC 43057 were the most favourable for C. annuum. The interactions between the different explants and strains showed significant differences with p-values < 0.0001 in both Capsicum species.

    CONCLUSIONS: Both Capsicum species were amenable to A. rhizogenes infection and hairy root induction is recommended for use as an alternative explants in future plant-based studies.

    Matched MeSH terms: Capsicum/growth & development*; Plant Roots/growth & development*; Hypocotyl/growth & development; Cotyledon/growth & development
  19. Noh A, Rafii MY, Mohd Din A, Kushairi A, Norziha A, Rajanaidu N, et al.
    Genet. Mol. Res., 2014;13(2):2426-37.
    PMID: 24781997 DOI: 10.4238/2014.April.3.15
    Twelve introgressed oil palm (Elaeis guineensis) progenies of Nigerian dura x Deli dura were evaluated for bunch yield, yield attributes, bunch quality components and vegetative characters at the Malaysian Palm Oil Board Research Station, in Keratong, Pahang, Malaysia. Analysis of variance revealed significant to highly significant genotypic differences, indicating sufficient genetic variability among the progenies for bunch yield and its attributes, vegetative characters and bunch quality components, except fruit to bunch ratio. Fresh fruit bunch yield ranged from 167 kg·palm(-1)·year(-1) in PK1330 to 212 kg·palm(-1)·year(-1) in PK1351, with a mean yield of 192 kg·palm(-1)·year(-1). Among the progeny, PK1313 had the highest oil to bunch ratio (19.36%), due to its high mesocarp to fruit ratio, fruit to bunch ratio and low shell to fruit ratio. Among the progenies, PK1313 produced the highest oil yield of 31.4 kg·palm(-1)·year(-1), due to a high mesocarp to fruit ratio (61.2%) and a low shell to fruit ratio (30.7%), coupled with high fruit to bunch ratio (65.6%). PK1330 was found promising for selection, as it had desirable vegetative characters, including smaller petiole cross section (27.15 cm2), short rachis length (4.83 m), short palm height (1.85 m), and the lowest leaf number (164.6), as these vegetative characters are prerequisites for selecting palms for high density planting and high yield per hectare. The genetic variability among the progenies was found to be high, indicating ample scope for further breeding, followed by selection.
    Matched MeSH terms: Plant Leaves/growth & development*; Arecaceae/growth & development
  20. Hossain MA, Ashrafuzzaman M, Hossain AK, Ismail MR, Koyama H
    ScientificWorldJournal, 2014;2014:457187.
    PMID: 24701169 DOI: 10.1155/2014/457187
    Aluminum (Al) sensitive wheat cultivar kalyansona was grown for 14 d in a range of Ca solution (125, 625, and 2500 μM) plus other nutrients without Al. At 14 d after Ca treatment, half of these plants were harvested (H1), and the rest of the plants were exposed to 100 μM Al for additional 6 d and harvested (H2). Severe Al injury was found only in the plants with the lowest supply of Ca before Al treatment. Aluminum concentration in the apoplastic fluid was very high at 125 μM Ca probably because the plasma membrane of some of the cells was destroyed due to the attack of 100 μM Al. Aluminum content in roots decreased with increasing supply of Ca before Al treatment. Calcium content decreased drastically at harvest (H2) in the plants with 100 μM Al. Under Al stress conditions, the plant responded to Al in different ways due to not only the different Ca supply but also the variation of Ca content in the plant tissues. Actually, the plants having the largest Ca content in the roots before Al treatment can receive less Al injury during Al treatment. To substantiate this idea, a companion study was conducted to investigate the effects of 2500 μM Ca supply during, before, and after 100 μM Al treatment on root growth. The results indicated clearly that exogenous Ca supply before Al treatment is able to alleviate Al injury but less effective than Ca supply during Al treatment.
    Matched MeSH terms: Triticum/growth & development*; Plant Roots/growth & development*
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