Displaying publications 81 - 98 of 98 in total

Abstract:
Sort:
  1. Fulltext Rare but Potentially Fatal Presentations of Diffuse Large B-cell Lymphoma: Leukemic Phase or Hemophagocytic Syndrome in Bone Marrow
    Wan Mohd Zohdi WA, Ismail AZ, Yusof N, Ithnin A, Shuib S, Masir N, et al.
    Clin Pathol, 2022 01 09;15:2632010X211070774.
    PMID: 35036912 DOI: 10.1177/2632010X211070774
    Diffuse large B-cell lymphoma (DLBCL) is a type of non-Hodgkin Lymphoma commonly presenting as a solid tumor either by nodal or extra-nodal manifestations. Here we describe two atypical presentations of lymphoma, finally resulting in the diagnosis of DLBCL. Case 1: A 53-year-old man with a previous history of nasopharyngeal carcinoma presented with a two-week history of B-symptoms and hyperleukocytosis. Peripheral blood film showed 78% abnormal mononuclear cells. Immunohistochemical stain showing Ki-67 of 90%, negative c-myc, BCL2 and BCL6, and negative c-MYC with fluorescence in-situ hybridization studies on the trephine biopsy, concluded the diagnosis of CD5+ DLBCL of ABC subtype. He received intravenous cyclophosphamide and oral prednisolone for cytoreduction, followed by 6 cycles of chemo-immunotherapy. However, he succumbed due to severe sepsis after the completion of therapy. Case 2: A 56-year-old lady who was initially investigated for pyrexia of unknown origin was noted to have hemophagocytosis upon bone marrow aspirate examination. The bone marrow trephine biopsy revealed some atypical clusters of B-cells positive for CD20 which was inconclusive. PET-CT scan noted an enlarged hypermetabolic spleen without lymphadenopathy. Splenic biopsy with immunohistochemical studies revealed DLBCL of ABC subtype. The diagnosis was consistent with primary splenic DLBCL. She became unwell post splenic biopsy and was admitted to the intensive care unit where she passed away 2 weeks later from Candida and Sternotrophomonas septicemia. These cases highlight the atypical presentations of a common subtype of NHL in our center. Arriving at the definitive diagnosis can be difficult especially when patients are acutely ill, hampering the necessary invasive procedures for diagnosis. The outcomes of both cases are briefly discussed hoping to spread awareness among clinicians on the rare and acutely critical presentations of DLBCL.
  2. Fulltext Emotional Intelligence, Depression, Stress and Anxiety Amongst Undergraduate Dental Students During the COVID-19 Pandemic
    Dasor MM, Jafridin AA, Azhar AA, Asma AAA, Manivannan PC, Bilal S, et al.
    Int J Public Health, 2023;68:1604383.
    PMID: 36846156 DOI: 10.3389/ijph.2023.1604383
    Objectives: This study aimed to measure depression, anxiety and stress (DAS) among undergraduate dental students during the COVID-19 pandemic, identify key contributing factors of stress and explore the association of emotional intelligence to DAS. Methods: A multi-centre, cross-sectional study was conducted across four universities in Malaysia. The study administered a questionnaire consisting of the validated Depression Anxiety Stress Scale (DASS), Dental Environment Stress (DES), Emotional Intelligence Scale (EI) and 10 statements assessing COVID-19 specific potential stressor factors. Results: Participants included 791 students across four universities. Abnormal levels of DAS were identified in 60.6%, 66.8% and 42.6% of the study participants, respectively. "Pressure of performance," "Faculty administration" and "Self-efficacy belief" were the highest rated stressors. On-time graduation was the main COVID-19 specific stressor factor. EI was negatively correlated with DAS scores (p < 0.001). Conclusion: The levels of DAS during COVID-19 pandemic in this population is high. However, participants with higher EI presented with lower DAS suggesting that EI may be a form of coping resource and should be enhanced in this population.
  3. Fulltext Antimicrobial resistance in methicillin-resistant staphylococcus aureus
    Ali Alghamdi B, Al-Johani I, Al-Shamrani JM, Musamed Alshamrani H, Al-Otaibi BG, Almazmomi K, et al.
    Saudi J Biol Sci, 2023 Apr;30(4):103604.
    PMID: 36936699 DOI: 10.1016/j.sjbs.2023.103604
    In the medical community, antibiotics are revered as a miracle because they stop diseases brought on by pathogenic bacteria. Antibiotics have become the cornerstone of contemporary medical advancements ever since penicillin was discovered. Antibiotic resistance developed among germs quickly, placing a strain in the medical field. Methicillin-resistant Staphylococcus aureus (MRSA), Since 1961, has emerged as the major general antimicrobial resistant bacteria (AMR) worldwide. MRSA can easily transmit across the hospital system and has mostly gained resistance to medications called beta-lactamases. This enzyme destroys the cell wall of beta-lactam antibiotics resulting in resistance against that respective antibiotic. Daptomycin, linezolid and vancomycin were previously used to treat MRSA infections. However, due to mutations and Single nucleotide polymorphisms (SNPs) in Open reading frames (ORFs) and SCCmec machinery of respective antibody, MRSA developed resistance against those antibiotics. The MRSA strains (USA300, CC398, CC130 etc.), when their pan-genomes were analyzed were found the genes involved in invoking resistance against the antibiotics as well as the epidemiology of that respective strain. PENC (penicillin plus potassium clavulanate) is the new antibiotic showing potential in treatment of MRSA though it is itself resistant against penicillin alone. In this review, our main focus is on mechanism of development of AMR in MRSA, how different ORFs are involved in evoking resistance in MRSA and what is the core-genome of different antimicrobial resistant MRSA.
  4. Fulltext Development of Copper-Aluminum Layered Double Hydroxide in Thin Film Nanocomposite Nanofiltration Membrane for Water Purification Process
    Tajuddin MH, Yusof N, Wan Azelee I, Wan Salleh WN, Ismail AF, Jaafar J, et al.
    Front Chem, 2019;7:3.
    PMID: 30800647 DOI: 10.3389/fchem.2019.00003
    This study aims to fabricate a thin film composite (TFC) membrane, modified with copper-aluminium layered double hydroxide (LDH) nanofillers via interfacial polymerization technique for nanofiltration (NF) processes. It was found that Cu-Al LDH nanofillers possessed layered structured materials with typical hexagonal plate-like shape and positive surface charge. The study revealed that TFN membrane exhibits a relatively smooth surface and a less nodular structure compared to pristine TFC membrane. The contact angle of TFN progressively decreased from 54.1° to 37.25°, indicating enhancement in surface hydrophilicity. Moreover, the incorporation of LDH nanofillers resulted in a less negative membrane as compared to the pristine TFC membrane. The best NF performance was achieved by TFN2 membrane with 0.1° of Cu-Al LDH loading and a water flux of 7.01 Lm-2h-1.bar. The addition of Cu-Al LDH resulted in excellent single salt rejections of Na2SO4 (96.8%), MgCl2 (95.6%), MgSO4 (95.4%), and NaCl (60.8%). The improvement in anti-fouling properties of resultant TFN membranes can be observed from the increments of pure water flux recovery and normalized water flux by 14% and 25% respectively. The findings indicated that Cu-Al LDH is a promising material in tailoring membrane surface properties and fouling resistance. The modification of the LDH-filled TFN membrane shows another alternative to fabricating a high-performance composite membrane, especially for water softening and partial desalination process.
  5. Virtual counselling of tobacco cessation during the COVID-19 pandemic: A qualitative study on the experiences and perceptions of Malaysian dental undergraduates and their patients
    Roslan N, Yusof N, Md Bohari NF, Md Sabri BA, Mohd Radzi NA, Bakri NN, et al.
    Eur J Dent Educ, 2024 Feb;28(1):28-40.
    PMID: 37132218 DOI: 10.1111/eje.12912
    INTRODUCTION: When dental institutions had to close down during the Movement Control Order (MCO) implementation due to the COVID-19 pandemic, dental students were faced with delays in completing their tobacco cessation schedule. An alternative was to allow students to conduct virtual counselling (VC) for smoking cessation for their patients to address their clinical requirements. This study aimed to explore Malaysian dental undergraduates' and patients' experiences undergoing smoking cessation counselling through virtual platforms.

    MATERIALS AND METHODS: The study consisted of qualitative, semi-structured Focus Group Discussions (for students, n = 23) and in-depth interviews (for patients, n = 9); to phenomenologically describe the perceptions of participants involved in the VC. Each session was recorded with the participants' permission. The recorded session was transcribed verbatim and thematically analysed using the qualitative data analysis software, NVivo™.

    RESULTS: The major themes that emerged were: (1) General opinions and experiences, (2) Content of VCs, (3) Remote access to counselling, (4) Patient-clinician relationships, (5) Technical issues, (6) Changes after VCs, and (7) Future application. Most students and patients were quite comfortable with VC as it is convenient, allowing students to be creative and avoid the hassle of transport and traffic. However, some of the students felt that it lacked the personal touch and guidance from lecturers who would normally be present during physical class.

    CONCLUSION: Virtual counselling enables remote access to counselling, but it is also subjected to some limitations, especially regarding lack of clinical assessments, human touch and internet issues. Though participants were optimistic about adapting it in the future, multiple factors must be considered. Ultimately, the behavioural change will depend on the patient's motivation in making a difference.

  6. Harnessing the photocatalytic potential of bismuth ferrite-activated carbon nanocomposite (BFO-AC) for Staphylococcus aureus decontamination under visible light
    Daub NA, Aziz F, Mhamad SA, Chee DNA, Jaafar J, Yusof N, et al.
    Environ Sci Pollut Res Int, 2024 Mar;31(11):16629-16641.
    PMID: 38321283 DOI: 10.1007/s11356-024-32261-w
    In response to the escalating global issue of microbial contamination, this study introduces a breakthrough photocatalyst: bismuth ferrite-activated carbon (BFO-AC) for visible light-driven disinfection, specifically targeting the Gram-positive bacterium Staphylococcus aureus (S. aureus). Employing an ultrasonication method, we synthesized various BFO-AC ratios and subjected them to comprehensive characterization. Remarkably, the bismuth ferrite-activated carbon 1:1.5 ratio (BA 1:1.5) nanocomposite exhibited the narrowest band gap of 1.86 eV. Notably, BA (1:1.5) demonstrated an exceptional BET surface area of 862.99 m2/g, a remarkable improvement compared to pristine BFO with only 27.61 m2/g. Further investigation through FE-SEM unveiled the presence of BFO nanoparticles on the activated carbon surface. Crucially, the photocatalytic efficacy of BA (1:1.5) towards S. aureus reached its zenith, achieving complete inactivation in just 60 min. TEM analysis revealed severe damage and rupture of bacterial cells, affirming the potent disinfection capabilities of BA (1:1.5). This exceptional disinfection efficiency underscores the promising potential of BA (1:1.5) for the treatment of contaminated water sources. Importantly, our results underscore the enhanced photocatalytic performance with an increased content of activated carbon, suggesting a promising avenue for more effective microorganism inactivation.
  7. Fulltext Enzymatic and genetic characterization of lignin depolymerization by Streptomyces sp. S6 isolated from a tropical environment
    Riyadi FA, Tahir AA, Yusof N, Sabri NSA, Noor MJMM, Akhir FNMD, et al.
    Sci Rep, 2020 05 08;10(1):7813.
    PMID: 32385385 DOI: 10.1038/s41598-020-64817-4
    The conversion of lignocellulosic biomass into bioethanol or biochemical products requires a crucial pretreatment process to breakdown the recalcitrant lignin structure. This research focuses on the isolation and characterization of a lignin-degrading bacterial strain from a decaying oil palm empty fruit bunch (OPEFB). The isolated strain, identified as Streptomyces sp. S6, grew in a minimal medium with Kraft lignin (KL) as the sole carbon source. Several known ligninolytic enzyme assays were performed, and lignin peroxidase (LiP), laccase (Lac), dye-decolorizing peroxidase (DyP) and aryl-alcohol oxidase (AAO) activities were detected. A 55.3% reduction in the molecular weight (Mw) of KL was observed after 7 days of incubation with Streptomyces sp. S6 based on gel-permeation chromatography (GPC). Gas chromatography-mass spectrometry (GC-MS) also successfully highlighted the production of lignin-derived aromatic compounds, such as 3-methyl-butanoic acid, guaiacol derivatives, and 4,6-dimethyl-dodecane, after treatment of KL with strain S6. Finally, draft genome analysis of Streptomyces sp. S6 also revealed the presence of strong lignin degradation machinery and identified various candidate genes responsible for lignin depolymerization, as well as for the mineralization of the lower molecular weight compounds, confirming the lignin degradation capability of the bacterial strain.
  8. Fulltext Exposure to environmental microbiota explains persistent abdominal pain and irritable bowel syndrome after a major flood
    Yusof N, Hamid N, Ma ZF, Lawenko RM, Wan Mohammad WMZ, Collins DA, et al.
    Gut Pathog, 2017;9:75.
    PMID: 29255490 DOI: 10.1186/s13099-017-0224-7
    Background: After an environmental disaster, the affected community is at increased risk for persistent abdominal pain but mechanisms are unclear. Therefore, our study aimed to determine association between abdominal pain and poor water, sanitation and hygiene (WaSH) practices, and if small intestinal bacterial overgrowth (SIBO) and/or gut dysbiosis explain IBS, impaired quality of life (QOL), anxiety and/or depression after a major flood.

    Results: New onset abdominal pain, IBS based on the Rome III criteria, WaSH practices, QOL, anxiety and/or depression, SIBO (hydrogen breath testing) and stools for metagenomic sequencing were assessed in flood victims. Of 211 participants, 37.9% (n = 80) had abdominal pain and 17% (n = 36) with IBS subtyped diarrhea and/or mixed type (n = 27 or 12.8%) being the most common. Poor WaSH practices and impaired quality of life during flood were significantly associated with IBS. Using linear discriminant analysis effect size method, gut dysbiosis was observed in those with anxiety (Bacteroidetes and Proteobacteria, effect size 4.8), abdominal pain (Fusobacteria, Staphylococcus, Megamonas and Plesiomonas, effect size 4.0) and IBS (Plesiomonas and Trabulsiella, effect size 3.0).

    Conclusion: Disturbed gut microbiota because of environmentally-derived organisms may explain persistent abdominal pain and IBS after a major environmental disaster in the presence of poor WaSH practices.

  9. Fulltext Draft Genome Sequence of the Prazosin-Degrading Bacillus sp. Strain PR5, Isolated from a River Receiving Hospital and Urban Wastewater in Malaysia
    Ahmad Sabri NS, Mohd Mohsi NF, Apandi A, Yusof N, Megat Mohd Noor MJ, Md Akhir FN, et al.
    Microbiol Resour Announc, 2021 Mar 18;10(11).
    PMID: 33737348 DOI: 10.1128/MRA.00025-21
    We report the complete genome sequence of Bacillus sp. strain PR5, isolated from a river receiving hospital and urban wastewater in Malaysia, which demonstrated a high capability for degrading prazosin. This genome sequence of 4,525,264 bp exhibited 41.5% GC content, 4,402 coding sequences, and 32 RNAs.
  10. Fulltext Complete Genome Sequence of Lignin-Degrading Streptomyces sp. Strain S6, Isolated from an Oil Palm Plantation in Malaysia
    Yusof N, Abdullah Tahir A, Riyadi FA, Ahmad Sabri NS, Md Akhir FN, Othman N, et al.
    Microbiol Resour Announc, 2020 Jan 30;9(5).
    PMID: 32001560 DOI: 10.1128/MRA.01332-19
    Streptomyces spp. are bacteria that are responsible for the degradation of aromatic compounds and produce secondary metabolites. Here, we present a complete genome sequence of Streptomyces sp. strain S6, which was isolated from an oil palm plantation, with a 7.8-Mbp liner chromosome, a GC content of 72%, and 4,266 coding sequences.
  11. Photocatalytic degradation of oilfield produced water using graphitic carbon nitride embedded in electrospun polyacrylonitrile nanofibers
    Alias NH, Jaafar J, Samitsu S, Yusof N, Othman MHD, Rahman MA, et al.
    Chemosphere, 2018 Aug;204:79-86.
    PMID: 29653325 DOI: 10.1016/j.chemosphere.2018.04.033
    Separation and purification of oilfield produced water (OPW) is a major environmental challenge due to the co-production of the OPW during petroleum exploration and production operations. Effective capture of oil contaminant and its in-situ photodegradation is one of the promising methods to purify the OPW. Based on the photocatalytic capability of graphitic carbon nitride (GCN) which was recently rediscovered, photodegradation capability of GCN for OPW was investigated in this study. GCN was synthesized by calcination of urea and further exfoliated into nanosheets. The GCNs were incorporated into polyacrylonitrile nanofibers using electrospinning, which gave a liquid-permeable self-supporting photocatalytic nanofiber mat that can be handled by hand. The photocatalytic nanofiber demonstrated 85.4% degradation of OPW under visible light irradiation, and improved the degradation to 96.6% under UV light. Effective photodegradation of the photocatalytic nanofiber for OPW originates from synergetic effects of oil adsorption by PAN nanofibers and oil photodegradation by GCNs. This study provides an insight for industrial application on purification of OPW through photocatalytic degradation under solar irradiation.
  12. Fulltext Antimicrobial Potential of Plastic Films Incorporated with Sage Extract on Chicken Meat
    Aziman N, Jawaid M, Mutalib NAA, Yusof NL, Nadrah AH, Nazatul UK, et al.
    Foods, 2021 Nov 16;10(11).
    PMID: 34829093 DOI: 10.3390/foods10112812
    The function of packaging is crucial in the maintenance of fresh meat product quality. This study aimed to assess the efficiency of six films added with coatings 2379L/220 and 2379L/221 (containing sage extracts) to inhibit Salmonella typhimurium, Staphylococcus aureus, and Escherichia coli, which showed that two of the six films had a significant effect. Additionally, the effects of the films on refrigerated skinless chicken breast meat were evaluated based on microbiological content, colour, weight loss, texture and pH. Four of the six films were examined could extend the storability of refrigerated chicken breast fillets for up to seven days. All six treated films improved the pH, colour stability, weight loss, and texture of the chicken fillets. Therefore, these findings suggested that the coatings containing sage extracts having different viscosities (2379L/220 and 2379L/221) were effective as antimicrobial adhesives in food packaging films and can be commercially applied in prolonging the storage of chicken breast meat without affecting their quality.
  13. Hydrous ferric oxide nanoparticles hosted porous polyethersulfone adsorptive membrane: chromium (VI) adsorptive studies and its applicability for water/wastewater treatment
    Abdullah N, Yusof N, Abu Shah MH, Wan Ikhsan SN, Ng ZC, Maji S, et al.
    Environ Sci Pollut Res Int, 2019 Jul;26(20):20386-20399.
    PMID: 31102226 DOI: 10.1007/s11356-019-05208-9
    In this present study, adsorptive membranes for Cr(VI) ion removal were prepared by blending polyethersulfone (PES) with hydrous ferric oxide (HFO) nanoparticles (NPs). The effects of HFO NPs to PES weight ratio (0-1.5) on the physicochemical properties of the resultant HFO/PES adsorptive membranes were investigated with respect to the surface chemistry and roughness as well as structural morphologies using different analytical instruments. The adsorptive performance of the HFO NPs/PES membranes was studied via batch adsorption experiments under various conditions by varying solution pH, initial concentration of Cr(VI), and contact time. The results showed that the membrane made of HFO/PES at a weight ratio of 1.0 exhibited the highest adsorption capacity which is 13.5 mg/g. Isotherm and kinetic studies revealed that the mechanism is best fitted to the Langmuir model and pseudo-second-order model. For filtration of Cr(VI), the best promising membranes showed improved water flux (629.3 L/m2 h) with Cr(VI) ion removal of 75%. More importantly, the newly developed membrane maintained the Cr(VI) concentration below the maximum contamination level (MCL) for up to 9 h.
  14. Immunophenotyping analysis of lymph node biopsies by flow cytometry
    Raja-Sabudin RZ, Hamid AA, Yusof N, Alauddin H, Aziz SA, Kulaveerasingam S, et al.
    Saudi Med J, 2012 Oct;33(10):1131-3.
    PMID: 23047221
  15. Fulltext A review of the potential of conventional and advanced membrane technology in the removal of pathogens from wastewater
    Nasir AM, Adam MR, Mohamad Kamal SNEA, Jaafar J, Othman MHD, Ismail AF, et al.
    Sep Purif Technol, 2022 Apr 01;286:120454.
    PMID: 35035270 DOI: 10.1016/j.seppur.2022.120454
    Consumption of pathogenic contaminated water has claimed the lives of many people. Hence, this scenario has emphasized the urgent need for research methods to avoid, treat and eliminate harmful pathogens in wastewater. Therefore, effective water treatment has become a matter of utmost importance. Membrane technology offers purer, cleaner, and pathogen-free water through the water separation method via a permeable membrane. Advanced membrane technology such as nanocomposite membrane, membrane distillation, membrane bioreactor, and photocatalytic membrane reactor can offer synergistic effects in removing pathogen through the integration of additional functionality and filtration in a single chamber. This paper also comprehensively discussed the application, challenges, and future perspective of the advanced membrane technology as a promising alternative in battling pathogenic microbial contaminants, which will also be beneficial and valuable in managing pandemics in the future as well as protecting human health and the environment. In addition, the potential of membrane technology in battling the ongoing global pandemic of coronavirus disease 2019 (COVID-19) was also discussed briefly.
  16. A Prospective Multicenter Randomized Controlled Trial to Evaluate the Efficacy of Chitosan Hydrogel Paste in Comparison to Commercial Hydroactive Gel as a Wound Bed Preparation
    Nasir NAM, Saad AZM, Bachok NS, Rashid AHA, Ujang Z, Noorsal K, et al.
    Indian J Plast Surg, 2023 Feb;56(1):44-52.
    PMID: 36998939 DOI: 10.1055/s-0042-1759503
    Background  This clinical trial aimed to evaluate the clinical efficacy of chitosan derivative hydrogel paste (CDHP) as a wound bed preparation for wounds with cavities. Methods  This study enrolled 287 patients, with 143 patients randomized into the CDHP group (treatment) and 144 patients randomized into the commercial hydroactive gel (CHG) group (control). The granulation tissue, necrotic tissue, patient comfort, clinical signs, symptoms, and patient convenience during the application and removal of the dressing were assessed. Results  The study was completed by 111 and 105 patients from the treatment and control groups, respectively. Both groups showed an increasing mean percentage of wound granulation over time when the initial wound size and comorbidity were adjusted (F(10,198) = 4.61; p  
  17. Fulltext Prevalence of Plasmodium falciparum Molecular Markers of Antimalarial Drug Resistance in a Residual Malaria Focus Area in Sabah, Malaysia
    Norahmad NA, Mohd Abd Razak MR, Abdullah NR, Sastu UR, Imwong M, Muniandy PK, et al.
    PLoS One, 2016;11(10):e0165515.
    PMID: 27788228 DOI: 10.1371/journal.pone.0165515
    Chloroquine (CQ) and fansidar (sulphadoxine-pyrimethamine, SP) were widely used for treatment of Plasmodium falciparum for several decades in Malaysia prior to the introduction of Artemisinin-based Combination Therapy (ACT) in 2008. Our previous study in Kalabakan, located in south-east coast of Sabah showed a high prevalence of resistance to CQ and SP, suggesting the use of the treatment may no longer be effective in the area. This study aimed to provide a baseline data of antimalarial drug resistant markers on P. falciparum isolates in Kota Marudu located in the north-east coast of Sabah. Mutations on genes associated with CQ (pfcrt and pfmdr1) and SP (pfdhps and pfdhfr) were assessed by PCR amplification and restriction fragment length polymorphism. Mutations on the kelch13 marker (K13) associated with artemisinin resistance were determined by DNA sequencing technique. The assessment of pfmdr1 copy number variation associated with mefloquine resistant was done by real-time PCR technique. A low prevalence (6.9%) was indicated for both pfcrt K76T and pfmdr1 N86Y mutations. All P. falciparum isolates harboured the pfdhps A437G mutation. Prevalence of pfdhfr gene mutations, S108N and I164L, were 100% and 10.3%, respectively. Combining the different resistant markers, only two isolates were conferred to have CQ and SP treatment failure markers as they contained mutant alleles of pfcrt and pfmdr1 together with quintuple pfdhps/pfdhfr mutation (combination of pfdhps A437G+A581G and pfdhfr C59R+S108N+I164L). All P. falciparum isolates carried single copy number of pfmdr1 and wild type K13 marker. This study has demonstrated a low prevalence of CQ and SP resistance alleles in the study area. Continuous monitoring of antimalarial drug efficacy is warranted and the findings provide information for policy makers in ensuring a proper malaria control.
  18. Fulltext Microbial Diversity in Decaying Oil Palm Empty Fruit Bunches (OPEFB) and Isolation of Lignin-degrading Bacteria from a Tropical Environment
    Tahir AA, Mohd Barnoh NF, Yusof N, Mohd Said NN, Utsumi M, Yen AM, et al.
    Microbes Environ, 2019 Jun 27;34(2):161-168.
    PMID: 31019143 DOI: 10.1264/jsme2.ME18117
    Oil palm empty fruit bunches (OPEFB) are the most abundant, inexpensive, and environmentally friendly lignocellulosic biomass in Malaysia. Investigations on the microbial diversity of decaying OPEFB may reveal microbes with complex enzymes that have the potential to enhance the conversion of lignocellulose into second-generation biofuels as well as the production of other value-added products. In the present study, fungal and bacterial diversities in decaying OPEFB were identified using Illumina MiSeq sequencing of the V3 region of the 16S rRNA gene and V4 region of the 18S rRNA gene. Fungal diversity in decaying OPEFB was dominated by the phylum Ascomycota (14.43%), while most of the bacterial sequences retrieved belonged to Proteobacteria (76.71%). Three bacterial strains isolated from decaying OPEFB, designated as S18, S20, and S36, appeared to grow with extracted OPEFB-lignin and Kraft lignin (KL) as the sole carbon source. 16S rRNA gene sequencing identified the 3 isolates as Paenibacillus sp.. The molecular weight distribution of KL before and after degradation showed significant depolymerization when treated with bacterial strains S18, S20, and S36. The presence of low-molecular-weight lignin-related compounds, such as vanillin and 2-methoxyphenol derivatives, which were detected by a GC-MS analysis, confirmed the KL-degrading activities of isolated Paenibacillus strains.
Related Terms
    Try searching for something
Filters
Contact Us

Please provide feedback to Administrator (afdal@afpm.org.my)

External Links