Familial hypercholesterolaemia (FH) is an autosomal dominant genetic disorder characterised by severe hypercholesterolaemia leading to premature coronary artery disease (CAD). Oxidised low-density lipoprotein (ox-LDL), F2-isoprostanes (ISP) and malondialdehyde (MDA) are established oxidative stress biomarker, but the status of oxidative stress in FH is not well studied. The aim of this study is to investigate oxidative stress status among FH patients and normocholesterolaemic control (NC) subjects. Ninety-eight FH patients and 100 (age, gender and BMI matched) NC subjects were recruited in series of health screening programmes across the country. Fasting blood samples were analysed for serum ox-LDL, ISP and MDA. Ox-LDL, ISP and MDA concentrations were higher in FH groups compared to NC (mean±SEM: 63.0±6.5 vs 25.5±1.2 (U/l), p
This study presents an efficient finite element analysis technique which shows great versatility in
modelling of precast composite flooring system subjected to static loadings. The method incorporates sliding and opening in the analysis of composite structures using the interface element which was specifically designed to simulate the actual behaviour at the interfaces between contacting materials. A three-dimensional finite element model of the precast composite slab which exhibits discontinuous behaviour was performed to demonstrate the potential and applicability of the proposed method of analysis. The results of the analysis demonstrate that the overall response of a discontinuous system to external loading is significantly affected by the bonding condition at the interfaces between the contacting materials.
The production of ethanol, from glucose in batch and fed batch culture, was investigated. In the fed batch culture, the glucose feeding was added into the culture at 16th hour of fermentation. The effects of different glucose concentration feeding rates on ethanol fermentation were investigated for fed batch culture. The 2gL-1hr-1 glucose concentration feeding rate was found to give higher ethanol yield (2.47 g ethanol g glucose-1), with respect to substrate consumed as compared to 8 gL-1hr-1 (0.23 g ethanol g glucose-1) and 4 gL-1hr-1 (0.20 g ethanol g glucose-1). The ethanol yield with respect to substrate consumed obtained in batch culture was 0.81 g ethanol g glucose-1. The fed batch culture at 2 gL-1hr-1 glucose concentration feeding rate was proven to be a better fermentation system than the batch culture. The specific growth rate, specific glucose consumption rate and specific ethanol production rate for the fed batch fermentation, at 2 gL-1hr-1 glucose concentration feeding rate, were 0.065 hr-1, 1.20 hr-1 and 0.0009 hr-1, respectively.
In this paper, we introduce T-DepExp system to simulate the transitive dependence based coalition formation (CF). It is a multi-agent based simulation (MABS) tool that aims to enhance cooperation between agents through transitive dependence. Previously, the transitive dependence was introduced by An and his colleagues for expressing the indirect dependence between agents in their cooperation. However, it did not receive much attention. Although it has a few problems need to be addressed, we try to propose our own mechanism to increase the efficiency of the transitive dependence based CF. To simulate MAS dependence relationship, we have included two fundamental dependence relationships in this MABS tool, which are AND-Dependence and OR-Dependence. In addition, the architecture of the T-DepExp system is presented and discussed. It allows possible integration of other features such as budget mechanism and trust model. Subsequently, hypothesis for the experiments and experimental setup are explained. The overall system will be demonstrated for its functionality and the experimental results will also be discussed.
Palm Oil Fuel Ash (POFA) is a biomass produced from palm oil industries. POFA is known to contain
a high amount of silica and has been proven by XRF spectroscopy, in which the silicon dioxide content
is 72.63%. In this study, silica was synthesised from POFA. To extract the silica, POFA was fused
with alkaline agent (Na2CO3) before mixing with Cethyltrimetyl Ammonium Bromide (CTAB) and
Sulphuric Acid (H2SO4). Sodium silicate solution from the fusion was used as silica precursor replacing
conventionally used silica source, Tetraethoxilane (TEOS). XRD pattern showed that raw POFA
dominantly consists of silica. Meanwhile, FTIR analysis of the synthesised silica exhibited spectra
bands at 3393 cm-1, 1635 cm-1, 1028 cm-1 and 787 cm-1 that corresponded to the functional groups of
Si-O and O-H. Thus, it could be concluded that silica was successfully extracted from POFA by the
alkaline fusion method.
The potential of Moringa oleifera Lam. (Moringaceae) and Centella asiatica (L.) Urban (Apiaceae) extracts (TGT-PRIMAAGE) in slowing the decline of memory and learning activity was investigated using D-galactose-induced ageing rat model. The extracts were profiled and standardised based on markers identified using LC/MS-QTOF. Toxicity study of the extract was done, and the rat did not show any sign of toxicity. The extract was orally administered to the rat and dose dependent (100, 500 and 1000 mg/kg) efficacy were investigated. The rats were subjected to Morris Water Maze whereby 3 parameters were studied (number of entry to platform, latency and novel object recognition). Plasma was collected for the determination of catalase (CAT) activity and levels of malondialdehyde (MDA) and advanced glycation end products (AGEs). The activity of acetylcholinesterase (AChE), level of acetylcholine (ACh) and lipid peroxidation (LPO) were measured using the brain lysates. Significant improvement (p < 0.05) was seen in the memory and learning abilities in the aged rats that received medium and high dose of TGT-PRIMAAGE, and tocotrienol. Rats treated with TGT-PRIMAAGE had also shown improved CAT activity and resulted in reduced LPO. The level of ACh was found increased in parallel with the reduced AChE activity. The capabilities of learning and memory of the TGT-PRIMAAGE treated rats were enhanced via inhibition of AChE activity and subsequently increased level of ACh.
Anaerobic composting is a promising method to fully transform food wastes into useful
materials such as biofertilizer and biogas. In this study, the optimum proportions of food
wastes containing vegetable, fruit and meat wastes with dry leaves or cow manure for
composting were determined using the simplex centroid design and response optimizer.
The effectiveness of the pilot-scale composting process was evaluated based on the targeted
compost quality of C/N ratio at 21, pH value at 8 and electrical conductivity of 1 dS/m.
Food wastes composting formulation with dry leaves suggested high percentage of dry
leaves, 86.9% with low food wastes composition of 13.1% constituted by vegetable waste
(1.1%), fruit waste (4.9%) and meat waste (7.1%). With cow manure formulation, only
6% of cow manure was recommended with
another 94.0% of food wastes contributed
by a fair mix of vegetable waste (23.2%),
fruit waste (34.3%) and meat waste (36.5%).
The developed regression models were
experimentally validated with predicted
responses obtained in acceptable ranges for
C/N ratio (21.2 - 21.8), pH (7.92 - 7.99) and
electrical conductivity (0.97 - 1.03 dS/m).
Fibre-rich manure derived from grass-fed cattle showed significantly higher intrinsic sorption efficiency on Cr(VI) solution as compared to corncob, sawdust and cogon grass. This observation could be attributed to the ligneous nature and rough surface morphology of the cattle manure. Four-factor, three-level, face-centred composite design (FCCD) suggested the process was greatly affected by initial pH of the solution, contact time and sorbent dosage (p50% adsorption efficiency. It is predicted that both physisorption and chemisorption are involved in the sorption process.
Concrete durability determines service life of structures. It can though, be weakened by aggressive environmental conditions. For instance, bio-corrosion process is due to the presence and activity of microorganisms which produce sulphuric acid to form sulphate deterioration of concrete materials. The problems related to durability and repair systems are due to lack of suitable concrete materials. The use bacteria for concrete repairing and plugging of pores and cracking in concrete has been recently explored. Previous studies had proved the possibility of using specific bacteria via bio concrete as a sustainable method for improving concrete properties. Thus, lack of information on the application of bio concrete exposed to extreme condition was the motivation for this research.
Many studies have been carried out using different metaheuristic algorithms on optimisation problems in various fields like engineering design, economics and routes planning. In the real world, resources and time are scarce. Thus the goals of optimisation algorithms are to optimise these available resources. Different metaheuristic algorithms are available. The firefly algorithm is one of the recent metaheuristic algorithms that is used in many applications; it is also modified and hybridised to improve its performance. In this paper, we compare the Standard Firefly Algorithm, the Elitist Firefly Algorithm, also called the Modified Firefly Algorithm with the Chaotic Firefly Algorithm, which embeds chaos maps in the Standard Firefly Algorithm. The Modified Firefly Algorithm differs from the Standard Firefly Algorithm in such a way that the global optimum solution at a particular iteration will not move randomly but in a direction that is chosen from randomly generated directions that can improve its performance. If none of these directions improves its performance, then the algorithm will not be updated. On the other hand, the Chaotic Firefly Algorithm tunes the parameters of the algorithms for the purpose of increasing the global search mobility i.e. to improve the attractiveness of fireflies. In our study, we found that the Chaotic Firefly Algorithms using three different chaotic maps do not perform as well as the Modified Firefly Algorithms; however, at least one or two of the Chaotic Firefly Algorithms outperform the Standard Firefly Algorithm under the given accuracy and efficiency tests.
This paper presents a two-staged, pilot-scale vertical flow engineered wetland-based septage treatment system (VFEWs), which was designed and constructed in Curtin University Sarawak Campus to determine the system efficiency in treatment of septage. The treatment system consists of storage tanks, vertical flow wetlands, and a network of influent and effluent distribution pipes. The first stage of the VFEWs treatment system consists of three vertical flow wetlands placed in parallel to provide pre-treatment to raw septage to reduce solids and organic matters mainly by physical filtration and sedimentation processes. The percolate from the first stage is then further treated in the second stage, with four vertical flow wetlands, each with variation in operational regime and substrate (filter) type. The influences of various system and application-related parameters such as substrate material, presence of plants and plant types, and septage feeding practices (solid loading rate (SLR), batch and intermittent loading, and frequency of daily feeding) on pollutant removal efficiency were studied. Results from the first stage wetlands indicate that the removal of total solids and organic matter (BOD and COD) from the raw septage is promising (> 80%) at both SLR of 100 kg TS/m2 .yr and 250 kg TS/m2 .yr, respectively. However, a higher SLR decreased the average NH3-N removal efficiency. The findings on bed clogging assessment during the study period are also presented in this paper. Validation and expansion of these results are carried out with ongoing assessments on the system performance.
In this study, a polluted site at Kg. Pasir Puteh was assessed for heavy metal pollution by using
transplanted caged mussel (Perna viridis) from a relatively clean population, Sg. Melayu; both are located in the Strait of Johore. For control purposes, the P. viridis from Kg. Pasir Puteh were also simultaneously transplanted in Sg. Melayu at the same time. It was found that Zn was the metal which got accumulated fastest in the transplanted mussel while Cd was the slowest. This study indicated that the byssus of Perna viridis was most effective for biomonitoring of Cd, Ni, Pb and Zn, while the shell could be used for the biomonitoring of Cu, Ni and Pb and the total soft tissue for the biomonitoring of Ni since they were able to accumulate and eliminate the respective metals well. By using mussel as a biomonitor, the present study found that Kg. Pasir Puteh, which is located in the eastern part of the Strait of Johore, had significantly higher contamination and bioavailabilities of Cd, Cu, Fe, Ni, Pb and Zn. Therefore, the use of the transplanted caged mussels is very useful for heavy metal assessment purposes since it can increase the validity of data interpretation by minimizing ecological factors.
The concentrations of cadmium, copper, zinc and lead, in the total soft tissues of green-lipped mussel Perna viridis of a wide range of sizes (2-11 cm), were determined from a population at Pasir Panjang. The metal contents (μg per individual) and concentrations (μg per g) of cadmium, lead, copper and zinc were studied in P. viridis to find the relationships with body sizes. Smaller and younger mussels showed higher concentrations (μg per g) of Cd, Pb and Zn than the larger and older ones. The results of the present study showed that the plotting of the metal content, against dry body flesh weight on a double logarithmic basis, gave good positive straight lines; this observation is in agreement with Boyden’s formula (1977). This indicated that P. viridis showed a different physiological strategy for each metal being studied, which is related to age.
Vapour pressure deficit (VPD) analysis introduces an approach to develop a better basis for the control of the environment of lowland greenhouses in Malaysia. The study of vapour pressure deficit (VPD) is to show air moisture conditions for plant production while taking into account different temperature levels. The purpose of this project is to develop a real-time automatic temperature and relative humidity control system in the lowland tropical greenhouse using a PIC16f876A microcontroller. The controller will then be used to monitor the temperature, relative humidity and VPD in the planting of Chili Kulai (Titisan 15). The fertigation system was introduced to the greenhouse to fertilize and irrigate the plant as well as to provide moisture to the environment. A swamp cooler was used to bring down the temperature and increase moisture content in the greenhouse. Ventilators were installed to remove the heat in the greenhouse. The study was carried out in an experimental greenhouse located at the Institute of Advanced Technology, Universiti Putra Malaysia (UPM).
Our continuing interest in anthraquinones from the Guttiferae family has led us to look at the genus Cratoxylum. A detailed chemical study on Cratoxylum aborescens resulted in the isolation of three anthraquinones, namely 1,8-dihydroxy-3-methoxy-6-methylanthraquinone (1), vismiaquinone (2) and vismione (3). These compounds were identified using 1D and 2D NMR spectroscopy. This is the first report on the chemistry of Cratoxylum aborescens.
Adequate and proper diabetes care in any practice is paramount and deems to be the fundamental requirement for good diabetes control. This is an audit of type 2 diabetes care process in a public Polyclinic, with the objectives of studying the quality of diabetic care provided in terms of clinical and managerial performance and also to give recommendations on improving its diabetes care delivery. The audit was done on patients' medical records selected via systematic random sampling. Patients who have been diagnosed with type 2 diabetes mellitus for more than 2 years, and those who have come for follow-up at least twice in between 1st October 2008 and 30th September 2009 by the medical officer and/or family medicine specialist. Patients with gestational diabetes mellitus and Type 1 diabetes mellitus were excluded. A total of 100 medical records were audited. 51% were female and 82% were in the 40-69 age group. Measurements of blood glucose and blood pressure were done at each follow-up at the polyclinic at 96% and 93% of the times, respectively. Within the past one year, HbA1c was performed in 46% of the patients, while renal profile was screened in 66% of the patients and urine protein was tested in 59% of the diabetics. Only 15% had their eyes screened by fundus camera. The diabetes care process at this public polyclinic was unsatisfactory, as many annual blood tests were not done and complications screening were also omitted. Thus, an urgent intervention is recommend in order to rectify these inadequacies.
Transformer failures lead to interruption of power supply. Therefore, asset management is important to monitor the efficient functioning of transformers. An important approach in asset management is condition assessment whereby the health status of the transformer is assessed via a health index. There are many methods in determining the final value of a health index. This paper examines how different assessment methods can be used in order to come up with the final health index and output of final health index. The output trend shapes are almost the same for Assessment Model A, B and C except for Assessment Model D. There is no strong correlation between the health index and age of the transformer. Generally, the value of health index of the transformer is reflected by its operation and loading history .This paper hence examines the assessment steps and results that will guide the development of a new approach to determine health index value.
Landfill leachate imposes a huge problem to the environment and human beings. This work focused on bioconversion of leachate to acetic and butyric acids by Clostridium butyricum NCIMB 7423. A continuous stirred tank reactor (CSTR) was applied and connected to fabricate membrane module. The leachate was collected from Pulau Burung Landfill Site (PBLS), Nibong Tebal, Penang. Prior to fermentation, leachate was treated to remove volatile fatty acid and adjusted to meet the minimum requirement of nutrients for anaerobic fermentation. Synthetic medium fermentation acts as a benchmark to the leachate fermentation. The outcomes indicated that the yield of acetic acid and butyric acid in synthetic medium fermentation was 0.70 g/L and 0.71 g/L, respectively. Meanwhile, leachate fermentation showed that the yield of acetic and butyric acid was 0.93 g/L and 1.86 g/L, respectively. High production of acetic and butyric acid showed that leachate fermentation is a green alternative to produce a cleaner product.
An experimental study was performed to determine the drying characteristics of dried papaya using different drying methods. They were dried using several methods, such as sun drying, solar drying, oven drying, and microwave drying. The effects of different operating conditions on physical quality attributes were investigated. The papaya were cut into different thicknesses and cooked in sugar syrup with different sugar concentration for 24 hours. Three different temperature settings were used in oven and microwave drying. The drying curve and drying rate of each method, temperature, sample
thickness and sugar concentration were studied. The drying times were found as in 6-15 minutes, 5 to 11 h, 10 to 18 h, and 14 to 23 h for the microwave, oven, sun and solar drying, respectively. The drying time increased with the increase of sample thickness and sugar concentration, as well as with the decrease of the drying temperature. In this study, quality attributes like colours and textural property of dried papaya were explored. Among the various methods of the drying characteristics
of papaya halwa, oven drying was preferred with the optimum sample in 5 mm thickness and at the air temperature of 70ºC as it saved up to 40% of the drying time as compared to other methods, except microwave, and produced acceptable physical quality of product.
The aim of this study was to compare physical activity prevalence estimates among the International Physical Activity Questionnaire (IPAQ), steps/day, and accelerometer in a sample of government employees in Kangar, Perlis, Malaysia. Ten government agencies in Kangar were randomly chosen, and all employees were invited to participate. A self-administered questionnaire was employed to obtain information on socio-demographic characteristics and a physical activity assessment using the IPAQ. Anthropometric measurements, which include measurements of weight, height, body mass index, percent body fat, waist and hip circumference, were carried out. An accelerometer was used to assess total daily energy expenditure and the number of steps/day. A total of 272 respondents were involved in this study with a response rate of 83.2%. According to IPAQ, accelerometer and steps/day, the majority of the respondents (22.0%, 55.1%, and 77.6%, respectively) were classified as sedentary. The agreement between physical activity level as determined by the accelerometer vs. the IPAQ (Kappa=-0.46 {95% CI -0.384,-0.536}, p=0.238) and the IPAQ vs. steps/day (Kappa =0.037 {95% CI 0.090,-0.016}, p=0.175) was not significant, but the agreement between physical activity level as determined by the accelerometer vs. steps/day was classified as fair (Kappa=0.296 (95% CI 0.392, 0.200}, p