Displaying publications 121 - 140 of 330 in total

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  1. Fulltext Nano-cellulose based nano-coating biomaterial dataset using corn leaf biomass: An innovative biodegradable plant biomaterial
    Sharif Hossain ABM, Uddin MM, Veettil VN, Fawzi M
    Data Brief, 2018 Apr;17:162-168.
    PMID: 29877503 DOI: 10.1016/j.dib.2017.12.046
    The nanocellulose derived biodegradable plant biomaterial as nano-coating can be used in the medical, biomedical cosmetics, and bioengineering products. Bio-plastic and some synthetic derived materials are edible and naturally biodegradable. The study was conducted to investigate edible nano-biopolymer based nano-coating of capsules and drugs or other definite biomedical materials from corn leaf biomass. Corn leaf biomass was used as an innovative sample to produce edible nano-coating bioplastic for drug and capsule coating and other industrial uses. The data show the negligible water 0.01% absorbed by bio-plastic nanocoating. Odor represented by burning test was under the completely standard based on ASTM. Moreover, data on color coating, tensile strength, pH, cellulose content have been shown under standard value of ASTM (American standard for testing and materials) standard. In addition to that data on the chemical element test like K+,


    CO


    3


    -
    -


    , Cl-, Na+ exhibited positive data compared to the synthetic plastic in the laboratory using the EN (166)) standardization. Therefore, it can be concluded that both organic (cellulose and starch) based edible nano-coating bioplastic may be used for drug and capsule coating as biomedical and medical components in the pharmaceutical industries.
    Matched MeSH terms: Biocompatible Materials
  2. An overview of application of silver nanoparticles for biomaterials in dentistry
    Bapat RA, Chaubal TV, Joshi CP, Bapat PR, Choudhury H, Pandey M, et al.
    Mater Sci Eng C Mater Biol Appl, 2018 Oct 01;91:881-898.
    PMID: 30033323 DOI: 10.1016/j.msec.2018.05.069
    Oral cavity is a gateway to the entire body and protection of this gateway is a major goal in dentistry. Plaque biofilm is a major cause of majority of dental diseases and although various biomaterials have been applied for their cure, limitations pertaining to the material properties prevent achievement of desired outcomes. Nanoparticle applications have become useful tools for various dental applications in endodontics, periodontics, restorative dentistry, orthodontics and oral cancers. Off these, silver nanoparticles (AgNPs) have been used in medicine and dentistry due to its antimicrobial properties. AgNPs have been incorporated into biomaterials in order to prevent or reduce biofilm formation. Due to greater surface to volume ratio and small particle size, they possess excellent antimicrobial action without affecting the mechanical properties of the material. This unique property of AgNPs makes these materials as fillers of choice in different biomaterials whereby they play a vital role in improving the properties. This review aims to discuss the influence of addition of AgNPs to various biomaterials used in different dental applications.
    Matched MeSH terms: Biocompatible Materials
  3. Efficient differentiation of human ES and iPS cells into cardiomyocytes on biomaterials under xeno-free conditions
    Sung TC, Liu CH, Huang WL, Lee YC, Kumar SS, Chang Y, et al.
    Biomater Sci, 2019 Oct 28.
    PMID: 31656967 DOI: 10.1039/c9bm00817a
    Current xeno-free and chemically defined methods for the differentiation of hPSCs (human pluripotent stem cells) into cardiomyocytes are not efficient and are sometimes not reproducible. Therefore, it is necessary to develop reliable and efficient methods for the differentiation of hPSCs into cardiomyocytes for future use in cardiovascular research related to drug discovery, cardiotoxicity screening, and disease modeling. We evaluated two representative differentiation methods that were reported previously, and we further developed original, more efficient methods for the differentiation of hPSCs into cardiomyocytes under xeno-free, chemically defined conditions. The developed protocol successively differentiated hPSCs into cardiomyocytes, approximately 90-97% of which expressed the cardiac marker cTnT, with beating speeds and sarcomere lengths that were similar to those of a healthy adult human heart. The optimal cell culture biomaterials for the cardiac differentiation of hPSCs were also evaluated using extracellular matrix-mimetic material-coated dishes. Synthemax II-coated and Laminin-521-coated dishes were found to be the most effective and efficient biomaterials for the cardiac differentiation of hPSCs according to the observation of hPSC-derived cardiomyocytes with high survival ratios, high beating colony numbers, a similar beating frequency to that of a healthy adult human heart, high purity levels (high cTnT expression) and longer sarcomere lengths similar to those of a healthy adult human heart.
    Matched MeSH terms: Biocompatible Materials
  4. The potential of dendrimer in delivery of therapeutics for dentistry
    Bapat RA, Dharmadhikari S, Chaubal TV, Amin MCIM, Bapat P, Gorain B, et al.
    Heliyon, 2019 Oct;5(10):e02544.
    PMID: 31687479 DOI: 10.1016/j.heliyon.2019.e02544
    Dendrimers are hyperbranched nanoparticle structures along with its surface modifications can to be used in dental biomaterials for biomimetic remineralisation of enamel and dentin. The review highlights the therapeutic applications of dendrimers in the field of dentistry. It addresses the possible mechanisms of enhancement of mechanical properties of adhesives and resins structure. Dendrimers due to its unique construction of possessing inner hydrophobic and outer hydrophilic structure can act as drug carrier for delivery of antimicrobial drugs for treatment of periodontal diseases and at peripheral dental implant areas. Dendrimers due to its hyperbranched structures can provides a unique drug delivery vehicle for delivery of a drug at specific site for sustained release for therapeutic effects. Thus, dendrimers can be one of the most important constituents which can be incorporated in dental biomaterials for better outcomes in dentistry.
    Matched MeSH terms: Biocompatible Materials
  5. An in vitro study to evaluate the genotoxicity of value added hydroxyapatite as a bone replacement material
    Akram Hassan, Swaminathan D
    Sains Malaysiana, 2011;40.
    Hydroxyapatite (HA) used for bone replacement is one of the most active areas of ceramic biomaterials research currently. It has been used clinically for the last 20 years due to its excellent biocompatibility, osseoconduction and osseointegration. Many modifications have been done to develop a stronger, tougher and biocompatible ceramic biomaterial because pure HA is brittle. Researchers in Universiti Sains Malaysia had developed this value added HA that is stronger and less brittle compared to pure HA. The objective of this in vitro study was to evaluate the genotoxic characteristic of the value added HA based material by using Bacterial Reverse Mutation Assay (Ames test). The Bacterial Reverse Mutation Assay of HA was performed on Salmonella typhimurium strains TA98, TA100, TA1535, TA1537 and Escherichia coli strain WP2 uvrA using the preincubation method in the presence and absence of an exogenous metabolic activation system. All the bacterial tester strains treated with and without S9 Mix showed no increase of revertant colonies with increase in concentration of test substance for both the dose finding test and the main test. The number of revertant colonies was less than twice that of the solvent control for all the five bacterial strains and this was reproducible for both the dose finding test and the main test. The numbers of revertant colonies in the negative and positive controls were within the background data of our laboratory. In conclusion the results of the tests showed that the value added HA was considered to have no reverse mutagenic potential under the present test conditions.
    Matched MeSH terms: Biocompatible Materials
  6. Preparation, characterization and properties of core-shell cobalt ferrite/polycaprolactone nanomagnetic biomaterials
    Khoo KS, Nur Farhana Amari, Tan CY, Shahidan Radiman, Redzuwan Yahaya, Muhamad Samudi Yasir
    Sains Malaysiana, 2013;42:167-173.
    Combination of magnetic and biocompatible materials to form core-shell nanomaterials has been widely used in medical fields. These core-shell magnetic biomaterials have a great potential for magnetic fluid hyperthermia (MFH) treatment to remedy cancer. The aims of this study were to investigate the production of core-shell cobalt ferrite/polycaprolactone (CoFe2O4/PCL) nanomaterials with different ratios of cobalt ferrite to caprolactone, to study the effects of using polymer in reducing the agglomerations between particles and to determine the structure, morphology, thermal and magnetic properties of these core-shell nanomaterials. The core-shell nanomaterials were produced by in situ polymerization method. The formation of the CoFe2O4/PCL was investigated by means of Fourier transform infrared spectroscopy (FTIR), x-ray diffractometer (XRD) and transmission electron microscopy (TEM). Its thermal properties were determined by using thermogravimetric analyzer (TGA). The vibrating sample magnetometer (VSM) was used to reveal the magnetic properties. The results for the XRD and FTIR spectra demonstrated the formation of cobalt ferrite and polycaprolactone in core-shell nanomaterials. From the TEM results, it was seen that the core-shell CoFe2O4/PCL nanomaterials were best formed at a ratio of CoFe2O4 to monomer caprolactone mixtures of 1:4.
    Matched MeSH terms: Biocompatible Materials
  7. Biomimetic hydroxyapatite/poly xylitol sebacic adibate/vitamin K nanocomposite for enhancing bone regeneration
    Dai Z, Dang M, Zhang W, Murugan S, Teh SW, Pan H
    Artif Cells Nanomed Biotechnol, 2019 Dec;47(1):1898-1907.
    PMID: 31066314 DOI: 10.1080/21691401.2019.1573183
    Hydroxyapatite (HAP) is a significant bone mineral that establishes bone strength. HAP composites in combination with biodegradable and bioactive polymer poly xylitol sebacic adipate (PXSA) would result in a constant release at target sites. Numerous studies have shown that vitamin K (VK) might possess a vital function in bone metabolism. The purpose of the present study was to inspect the synthesized composite HAP/PXSA/VK in developing polymeric biomaterials composite for the application of bone tissue regeneration. FTIR, X-ray diffraction, SEM and TEM techniques were applied to characterize the prepared composites. The release of VK from the HAP/PXSA/VK composite was evidenced through UV-Vis spectroscopy. In vitro studies proved that the HAP/PXSA/VK composite is appropriate for mesenchymal stem cell culture. Compared to pure HAP prepared following the same method, HAP/PXSA/VK composite provided favourable microstructures and good biodegradation distinctiveness for the application of tissue engineering, as well as tissue in-growth characteristics and improved scaffold cell penetration. This work reveals that the HAP/PXSA/VK composites have the potential for applications in bone tissue engineering.
    Matched MeSH terms: Biocompatible Materials
  8. Electrospun poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)/silk fibroin film is a promising scaffold for bone tissue engineering
    Ang SL, Shaharuddin B, Chuah JA, Sudesh K
    Int J Biol Macromol, 2020 Feb 15;145:173-188.
    PMID: 31866541 DOI: 10.1016/j.ijbiomac.2019.12.149
    Polyhydroxyalkanoates (PHAs) are biodegradable polyesters produced by microorganisms, under unbalanced growth conditions, as a carbon storage compound. PHAs are composed of various monomers such as 3-hydroxybutyrate (3HB) and 3-hydroxyhexanoate (3HHx). Silk fibroin (SF) derived from Bombyx mori cocoons, is a widely studied protein polymer commonly used for biomaterial applications. In this study, non-woven electrospun films comprising a copolymer of 3HB and 3HHx [P(3HB-co-3HHx)], SF and their blends were prepared by electrospinning technique. The growth and osteogenic differentiation of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) were studied using different types of fabricated electrospun films. The differentiation study revealed that electrospun P(3HB-co-3HHx)/SF film supports the differentiation of hUC-MSCs into the osteogenic lineage, confirmed by histological analysis using Alizarin Red staining, energy dispersive X-ray (EDX) and quantitative real-time PCR analysis (qPCR). Electrospun P(3HB-co-3HHx)/SF film up-regulated the expression of osteogenic marker genes, alkaline phosphatase (ALP) and osteocalcin (OCN), by 1.6-fold and 2.8-fold respectively, after 21 days of osteogenic induction. In conclusion, proliferation and osteogenic differentiation of hUC-MSCs were enhanced through the blending of P(3HB-co-3HHx) and SF. The results from this study suggest that electrospun P(3HB-co-3HHx)/SF film is a promising biomaterial for bone tissue engineering.
    Matched MeSH terms: Biocompatible Materials
  9. Fulltext Titanium and titanium based alloys as metallic biomaterials in medical applications – spine implant case study
    Nur Azida Che Lah, Muhamad Hellmy Hussin
    Pertanika Journal of Science & Technology, 2019;27(1):459-472.
    MyJurnal
    Titanium (Ti) and Ti-based alloys presence the most widely applied as advanced biomaterials
    in biomedical implant applications. Moreover, these alloys are known to be the most
    valuable metallic materials including spinal cord surgical treatment. It becomes an interest
    due to its advantages compared to others, including its bio compatibility and corrosion
    resistant. However, an issue arises when it comes for permanent implant application as
    the alloy has a possible toxic effect produced from chemical reaction between body fluid
    environments with alloys chemical compositions. It also relies on the performance of
    neighbouring bone tissue to integrate with the implant surface. Abnormalities usually
    happen when surrounding tissue shows poor responses and rejection of implants that would
    leads to body inflammation. These cause an increase in foreign body reaction leading to
    severe body tissue response and thus, loosening of the implant. Corrosion effects and
    biocompatibility behaviour of implantation usage also become one of the reasons of
    implant damage. Here, this paper reviews the importance of using Ti and Ti-based alloys
    in biomedical implantation, especially in orthopaedic spinal cord injury. It also reviews the
    basic aspects of corrosion effects that lead to implant mechanical damage, poor response
    of body rejection and biocompatibility behaviour of implantation usage.
    Matched MeSH terms: Biocompatible Materials
  10. XPS study of sulfur and phosphorus compounds with different oxidation states
    Leanne Britcher, Sunil Kumar, Hans J. Griesser, Kim S. Siow
    Sains Malaysiana, 2018;47:1913-1922.
    In this report, we demonstrate that continuous improvement in XPS instruments and the calibration standards as well
    as analysis with standard component-fitting procedures can be used to determine the binding energies of compounds
    containing phosphorus and sulfur of different oxidation states with higher confidence. Based on such improved XPS
    analyses, the binding energies (BEs) of S2p signals for sulfur of increasing oxidation state are determined to be 166-167.5
    eV for S=O in dimethyl sulfoxide, 168.1 eV for S=O2
    in polysulfone, 168.4 eV for SO3
    in polystyrene sulfonate and 168.8
    eV for SO4
    in chondroitin sulfate. The BEs of P2p signals show the following values: 132.9 eV for PO3
    in triisopropyl
    phosphite, 133.3 eV for PO4
    in glycerol phosphate, 133.5 eV for PO4
    in sodium tripolyphosphate and 134.0 eV for PO4
    in sodium hexametaphosphate. These results showed that there are only small increases in the binding energy when
    additional oxygen atoms are added to the S-O chemical group. A similar result is obtained when the fourth oxygen or
    poly-phosphate environment is added to the phosphorus compound. These BE values are useful to researchers involved
    in identifying oxidation states of phosphorus and sulfur atoms commonly observed on modified surfaces and interfaces
    found in applications such as biomaterials, super-capacitors and catalysis.
    Matched MeSH terms: Biocompatible Materials
  11. Fulltext Mechanism of Fatigue Crack Growth in Biomedical Alloy Ti-27Nb
    Amjad M, Badshah S, Rafique AF, Adil Khattak M, Khan RU, Abdullah Harasani WI
    Materials (Basel), 2020 May 16;13(10).
    PMID: 32429420 DOI: 10.3390/ma13102299
    Implants are widely used in the human body for the replacement of affected bones. Fatigue failure is one of the serious concerns for implants. Therefore, understanding of the underlying mechanism leading to fatigue failure is important for the longevity of biomaterial implants. In this paper, the fracture toughness and fatigue crack growth of titanium alloy biomaterial Ti-27Nb has been experimentally investigated. The Ti-27Nb material is tested for fatigue crack growth in different environmental conditions representing the ambient and in vitro environments for 504 hours and 816 hours, respectively. Fractography of the tested specimen is conducted using Scanning Electron Microscope (SEM). The results of the fatigue crack growth propagation of the ambient and in vitro samples are similar in the Paris crack growth region. However, in the threshold region, the crack growth rate is higher for the Simulated Body Fluid (SBF) treated specimen. The fracture surface morphology of in vitro samples shows brittle fracture as compared to ambient specimens with significant plasticity and striations marks. It is proposed that a similar investigation may be conducted with specimens treated in SBF for prolonged periods to further ascertain the findings of this study.
    Matched MeSH terms: Biocompatible Materials
  12. A dual-application poly (dl-lactic-co-glycolic) acid (PLGA)-chitosan composite scaffold for potential use in bone tissue engineering
    Boukari Y, Qutachi O, Scurr DJ, Morris AP, Doughty SW, Billa N
    J Biomater Sci Polym Ed, 2017 Nov;28(16):1966-1983.
    PMID: 28777694 DOI: 10.1080/09205063.2017.1364100
    The development of patient-friendly alternatives to bone-graft procedures is the driving force for new frontiers in bone tissue engineering. Poly (dl-lactic-co-glycolic acid) (PLGA) and chitosan are well-studied and easy-to-process polymers from which scaffolds can be fabricated. In this study, a novel dual-application scaffold system was formulated from porous PLGA and protein-loaded PLGA/chitosan microspheres. Physicochemical and in vitro protein release attributes were established. The therapeutic relevance, cytocompatibility with primary human mesenchymal stem cells (hMSCs) and osteogenic properties were tested. There was a significant reduction in burst release from the composite PLGA/chitosan microspheres compared with PLGA alone. Scaffolds sintered from porous microspheres at 37 °C were significantly stronger than the PLGA control, with compressive strengths of 0.846 ± 0.272 MPa and 0.406 ± 0.265 MPa, respectively (p 
    Matched MeSH terms: Biocompatible Materials/metabolism; Biocompatible Materials/pharmacology*; Biocompatible Materials/chemistry*
  13. 100 Years of the Journal of Dental Research: A Bibliometric Analysis
    Ahmad P, Alam MK, Jakubovics NS, Schwendicke F, Asif JA
    J Dent Res, 2019 Dec;98(13):1425-1436.
    PMID: 31746684 DOI: 10.1177/0022034519880544
    Since its inception in 1919, the Journal of Dental Research has continually published high-quality articles that span the breadth of research topics relevant to dentistry, oral surgery, and medicine. As part of the journal's centennial celebration, we conducted an electronic search on Scopus to identify and analyze the top 100 most cited articles from 1919 to 2018. Since Scopus does not capture older citations, we conducted an additional analysis by Google Scholar to identify key articles published in the first 50 y of the journal. Based on Scopus, the articles were ranked in descending order per their citation counts. The citation counts of the 100 most cited articles varied from 262 to 1,503. The year in which the largest number of top 100 articles were published was 2004 (n = 6). Within the top 100, the majority of articles originated from the United States (n = 52). Research Reports-Biomaterials & Bioengineering was the most frequent category of cited articles (n = 35). There was no significant association between total citation count and time since publication (correlation coefficient = -0.051, P = 0.656). However, there was a significant negative association of citation density (correlation coefficient = -0.610, P < 0.01) with time since publication. Our analyses demonstrate the broad reach of the journal and the dynamics in citation patterns and research agenda over its 100-y history. There is considerable evidence of the high variance in research output, when measured via citations, across the globe. Moreover, it remains unclear how patients' priorities and dental health care needs are aligned with the perceived influence of single research pieces identified by our search. Our findings may help to inspire future research in tackling these inequalities and highlight the need for conceptualizing research priorities.
    Matched MeSH terms: Biocompatible Materials
  14. Current appraises of therapeutic applications of nanocurcumin: A novel drug delivery approach for biomaterials in dentistry
    Bapat RA, Bedia SV, Bedia AS, Yang HJ, Dharmadhikari S, Abdulla AM, et al.
    Environ Res, 2023 Dec 01;238(Pt 1):116971.
    PMID: 37717805 DOI: 10.1016/j.envres.2023.116971
    Curcumin is a natural herb and polyphenol that is obtained from the medicinal plant Curcuma longa. It's anti-bacterial, anti-inflammatory, anti-cancer, anti-mutagenic, antioxidant and antifungal properties can be leveraged to treat a myriad of oral and systemic diseases. However, natural curcumin has weak solubility, limited bioavailability and undergoes rapid degradation, which severely limits its therapeutic potential. To overcome these drawbacks, nanocurcumin (nCur) formulations have been developed for improved biomaterial delivery and enhanced treatment outcomes. This novel biomaterial holds tremendous promise for the treatment of various oral diseases, the majority of which are caused by dental biofilm. These include dental caries, periodontal disease, root canal infection and peri-implant diseases, as well as other non-biofilm mediated oral diseases such as oral cancer and oral lichen planus. A number of in-vitro studies have demonstrated the antibacterial efficacy of nCur in various formulations against common oral pathogens such as S. mutans, P. gingivalis and E. faecalis, which are strongly associated with dental caries, periodontitis and root canal infection, respectively. In addition, some clinical studies were suggestive of the notion that nCur can indeed enhance the clinical outcomes of oral diseases such as periodontitis and oral lichen planus, but the level of evidence was very low due to the small number of studies and the methodological limitations of the available studies. The versatility of nCur to treat a diverse range of oral diseases augurs well for its future in dentistry, as reflected by rapid pace in which studies pertaining to this topic are published in the scientific literature. In order to keep abreast of the latest development of nCur in dentistry, this narrative review was undertaken. The aim of this narrative review is to provide a contemporaneous update of the chemistry, properties, mechanism of action, and scientific evidence behind the usage of nCur in dentistry.
    Matched MeSH terms: Biocompatible Materials
  15. Chitosan and hyaluronic acid-based nanocarriers for advanced cancer therapy and intervention
    Rohtagi P, Garg U, Triveni, Jain N, Pandey M, Amin MCIM, et al.
    Biomater Adv, 2024 Feb;157:213733.
    PMID: 38118207 DOI: 10.1016/j.bioadv.2023.213733
    Cancer has become a major public health issue leading to one of the foremost causes of morbidity and death in the world. Despite the current advances in diagnosis using modern technologies and treatment via surgery or chemo- and radio-therapies, severe side effects or after-effects limit the application of these treatment modalities. Novel drug delivery systems have shown the potential to deliver chemotherapeutics directly to cancer cells, thus minimizing unnecessary exposure to healthy cells. Concurrently, to circumvent difficulties associated with conventional deliveries of cancer therapeutics, natural polysaccharides have gained attention for the fabrication of such deliveries owing to biocompatibility, low toxicity, and biodegradability. It has been exhibited that natural polysaccharides can deliver high therapeutic concentrations of the entrapped drug to the target cells by sustained and targeted release. Considering the immense potential of natural polymers, the present work focuses on naturally generated biopolymer carriers based on chitosan and hyaluronic acid. This review delineated on the role of chitosan and its derivation from renewable resources as a biocompatible, biodegradable, nonimmunogenic material with notable antitumor activity as a drug delivery carrier in oncotherapy. Moreover, hyaluronic acid, itself by its structure or when linked with other molecules contributes to developing promising pharmaceutical delivery systems to setback the restrictions related to conventional cancer treatment.
    Matched MeSH terms: Biocompatible Materials
  16. A novel chitosan-alginate@Fe/Mn mixed oxide nanocomposite for highly efficient removal of Cr (VI) from wastewater: Experiment and adsorption mechanism
    Alqarni LS, Algethami JS, El Kaim Billah R, Alorabi AQ, Alnaam YA, Algethami FK, et al.
    Int J Biol Macromol, 2024 Apr;263(Pt 2):129989.
    PMID: 38354916 DOI: 10.1016/j.ijbiomac.2024.129989
    In this study, the synthesis and experimental theoretical evaluation of a new chitosan/alginate/hydrozyapatite nanocomposite doped with Mn2 and Fe2O3 for Cr removal was reported. The physicochemical properties of the obtained materials were analyzed using the following methods: SEM-EDX, XRD, FTIR, XPS, pH drift measurements, and thermal analysis. The adsorption properties were estimated based on equilibrium and adsorption kinetics measurements. The Langmuir, Freundlich and Temkin isotherms were applied to analyze the equilibrium data. The thermodynamic analysis of adsorption isotherms was performed. A number of equations and kinetic models were used to describe the adsorption rate data, including pseudo-first (PFOE) and pseudo-second (PSOE) order kinetic equations. The obtained test results show that the synthesized biomaterial, compared to pure chitosan, is characterized by greater resistance to high temperatures. Moreover, this biomaterial had excellent adsorption properties. For the adsorption of Cr (VI), the equilibrium state was reached after 120 min, and the sorption capacity was 455.9 mg/g. In addition, DFT calculations and NCI analyses were performed to get more light on the adsorption mechanism of Cr (VI) on the prepared biocomposite.
    Matched MeSH terms: Biocompatible Materials
  17. Fulltext Attachment, Proliferation, and Morphological Properties of Human Dermal Fibroblasts on Ovine Tendon Collagen Scaffolds: A Comparative Study
    Busra FM, Lokanathan Y, Nadzir MM, Saim A, Idrus RBH, Chowdhury SR
    Malays J Med Sci, 2017 Mar;24(2):33-43.
    PMID: 28894402 DOI: 10.21315/mjms2017.24.2.5
    INTRODUCTION: Collagen type I is widely used as a biomaterial for tissue-engineered substitutes. This study aimed to fabricate different three-dimensional (3D) scaffolds using ovine tendon collagen type I (OTC-I), and compare the attachment, proliferation and morphological features of human dermal fibroblasts (HDF) on the scaffolds.

    METHODS: This study was conducted between the years 2014 to 2016 at the Tissue Engineering Centre, UKM Medical Centre. OTC-I was extracted from ovine tendon, and fabricated into 3D scaffolds in the form of sponge, hydrogel and film. A polystyrene surface coated with OTC-I was used as the 2D culture condition. Genipin was used to crosslink the OTC-I. A non-coated polystyrene surface was used as a control. The mechanical strength of OTC-I scaffolds was evaluated. Attachment, proliferation and morphological features of HDF were assessed and compared between conditions.

    RESULTS: The mechanical strength of OTC-I sponge was significantly higher than that of the other scaffolds. OTC-I scaffolds and the coated surface significantly enhanced HDF attachment and proliferation compared to the control, but no differences were observed between the scaffolds and coated surface. In contrast, the morphological features of HDF including spreading, filopodia, lamellipodia and actin cytoskeletal formation differed between conditions.

    CONCLUSION: OTC-I can be moulded into various scaffolds that are biocompatible and thus could be suitable as scaffolds for developing tissue substitutes for clinical applications and in vitro tissue models. However, further study is required to determine the effect of morphological properties on the functional and molecular properties of HDF.

    Matched MeSH terms: Biocompatible Materials
  18. In vitro and in vivo study of hazardous effects of Ag nanoparticles and Arginine-treated multi walled carbon nanotubes on blood cells: application in hemodialysis membranes
    Zare-Zardini H, Amiri A, Shanbedi M, Taheri-Kafrani A, Kazi SN, Chew BT, et al.
    J Biomed Mater Res A, 2015 Sep;103(9):2959-65.
    PMID: 25690431 DOI: 10.1002/jbm.a.35425
    One of the novel applications of the nanostructures is the modification and development of membranes for hemocompatibility of hemodialysis. The toxicity and hemocompatibility of Ag nanoparticles and arginine-treated multiwalled carbon nanotubes (MWNT-Arg) and possibility of their application in membrane technology are investigated here. MWNT-Arg is prepared by amidation reactions, followed by characterization by FTIR spectroscopy, Raman spectroscopy, and thermogravimetric analysis. The results showed a good hemocompatibility and the hemolytic rates in the presence of both MWNT-Arg and Ag nanoparticles. The hemolytic rate of Ag nanoparticles was lower than that of MWNT-Arg. In vivo study revealed that Ag nanoparticle and MWNT-Arg decreased Hematocrit and mean number of red blood cells (RBC) statistically at concentration of 100 µg mL(-1) . The mean decrease of RBC and Hematocrit for Ag nanoparticles (18% for Hematocrit and 5.8 × 1,000,000/µL) was more than MWNT-Arg (20% for Hematocrit and 6 × 1000000/µL). In addition, MWNT-Arg and Ag nanoparticles had a direct influence on the White Blood Cell (WBC) drop. Regarding both nanostructures, although the number of WBC increased in initial concentration, it decreased significantly at the concentration of 100 µg mL(-1) . It is worth mentioning that the toxicity of Ag nanoparticle on WBC was higher than that of MWNT-Arg. Because of potent antimicrobial activity and relative hemocompatibility, MWNT-Arg could be considered as a new candidate for biomedical applications in the future especially for hemodialysis membranes.
    Matched MeSH terms: Biocompatible Materials/toxicity; Biocompatible Materials/chemistry
  19. Fulltext Enhanced blood compatibility of metallocene polyethylene subjected to hydrochloric acid treatment for cardiovascular implants
    Jaganathan SK, Mohandas H, Sivakumar G, Kasi P, Sudheer T, Avineri Veetil S, et al.
    Biomed Res Int, 2014;2014:963149.
    PMID: 24955370 DOI: 10.1155/2014/963149
    Blood compatibility of metallocene polyethylene (mPE) was investigated after modifying the surface using hydrochloric acid. Contact angle of the mPE exposed to HCl poses a decrease in its value which indicates increasing wettability and better blood compatibility. Surface of mPE analyzed by using FTIR revealed no significant changes in its functional groups after treatment. Furthermore, scanning electron microscope images supported the increasing wettability through the modifications like pit formations and etching on the acid rendered surface. To evaluate the effect of acid treatment on the coagulation cascade, prothrombin time (PT) and activated partial thromboplastin time (APTT) were measured. Both PT and APTT were delayed significantly (P < 0.05) after 60 min exposure implying improved blood compatibility of the surfaces. Hemolysis assay of the treated surface showed a remarkable decrease in the percentage of lysis of red blood cells when compared with untreated surface. Moreover, platelet adhesion assay demonstrated that HCl exposed surfaces deter the attachment of platelets and thereby reduce the chances of activation of blood coagulation cascade. These results confirmed the enhanced blood compatibility of mPE after HCl exposure which can be utilized for cardiovascular implants like artificial vascular prostheses, implants, and various blood contacting devices.
    Matched MeSH terms: Biocompatible Materials/therapeutic use; Biocompatible Materials/chemistry
  20. Fulltext Engineered silybin nanoparticles educe efficient control in experimental diabetes
    Das S, Roy P, Pal R, Auddy RG, Chakraborti AS, Mukherjee A
    PLoS One, 2014;9(7):e101818.
    PMID: 24991800 DOI: 10.1371/journal.pone.0101818
    Silybin, is one imminent therapeutic for drug induced hepatotoxicity, human prostate adenocarcinoma and other degenerative organ diseases. Recent evidences suggest that silybin influences gluconeogenesis pathways favorably and is beneficial in the treatment of type 1 and type 2 diabetes. The compound however is constrained due to solubility (0.4 mg/mL) and bioavailabilty limitations. Appropriate nanoparticle design for silybin in biocompatible polymers was thus proposed as a probable solution for therapeutic inadequacy. New surface engineered biopolymeric nanoparticles with high silybin encapsulation efficiency of 92.11% and zeta potential of +21 mV were designed. Both the pure compound and the nanoparticles were evaluated in vivo for the first time in experimental diabetic conditions. Animal health recovered substantially and the blood glucose levels came down to near normal values after 28 days treatment schedule with the engineered nanoparticles. Restoration from hyperglycemic damage condition was traced to serum insulin regeneration. Serum insulin recovered from the streptozotocin induced pancreatic damage levels of 0.17 ± 0.01 µg/lit to 0.57 ± 0.11 µg/lit after nanoparticle treatment. Significant reduction in glycated hemoglobin level, and restoration of liver glycogen content were some of the other interesting observations. Engineered silybin nanoparticle assisted recovery in diabetic conditions was reasoned due to improved silybin dissolution, passive transport in nanoscale, and restoration of antioxidant status.
    Matched MeSH terms: Biocompatible Materials/administration & dosage*; Biocompatible Materials/pharmacokinetics
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