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Urinary NMR-based metabolomic analysis of rats possessing variable sperm count following orally administered Eurycoma longifolia extracts of different quassinoid levels

Ebrahimi F, Ibrahim B, Teh CH, Murugaiyah V, Chan KL

J Ethnopharmacol, 2016 Apr 22;182:80-9.
PMID: 26899442 DOI: 10.1016/j.jep.2016.02.015

Eurycoma longifolia (Tongkat Ali, TA) roots have been ethnically used as a remedy to boost male sexual desire, libido, energy and fertility.
NMR-based plasma metabolomic discrimination for male fertility assessment of rats treated with Eurycoma longifolia extracts

Ebrahimi F, Ibrahim B, Teh CH, Murugaiyah V, Chan KL

Syst Biol Reprod Med, 2017 Jun;63(3):179-191.
PMID: 28306342 DOI: 10.1080/19396368.2017.1295332

Male infertility is one of the leading causes of infertility which affects many couples worldwide. Semen analysis is a routine examination of male fertility status which is usually performed on semen samples obtained through masturbation that may be inconvenient to patients. Eurycoma longifolia (Tongkat Ali, TA), native to Malaysia, has been traditionally used as a remedy to boost male fertility. In our recent studies in rats, upon the administration of high-quassinoid content extracts of TA including TA water (TAW), quassinoid-rich TA (TAQR) extracts, and a low-quassinoid content extract including quassinoid-poor TA (TAQP) extract, sperm count (SC) increased in TAW- and TAQR-treated rats when compared to the TAQP-treated and control groups. Consequently, the rats were divided into normal- (control and TAQP-treated) and high- (TAW- and TAQR-treated) SC groups [Ebrahimi et al. 2016]. Post-treatment rat plasma was collected. An optimized plasma sample preparation method was developed with respect to the internal standards sodium 3- (trimethylsilyl) propionate- 2,2,3,3- d4 (TSP) and deuterated 4-dimethyl-4-silapentane-1-ammonium trifluoroacetate (DSA). Carr-Purcell-Meibum-Gill (CPMG) experiments combined with orthogonal partial least squares discriminant analysis (OPLS-DA) was employed to evaluate plasma metabolomic changes in normal- and high-SC rats. The potential biomarkers associated with SC increase were investigated to assess fertility by capturing the metabolomic profile of plasma. DSA was selected as the optimized internal standard for plasma analysis due to its significantly smaller half-height line width (W h/2) compared to that of TSP. The validated OPLS-DA model clearly discriminated the CPMG profiles in regard to the SC level. Plasma profiles of the high-SC group contained higher levels of alanine, lactate, and histidine, while ethanol concentration was significantly higher in the normal-SC group. This approach might be a new alternative applicable to the fertility assessment in humans through the quantitative metabolomic analysis of plasma without requiring semen.
ABBREVIATIONS: TA: Tongkat Ali; LOD: limit of detection; LOQ: limit of quantification; HPLC-UV: high performance liquid chromatography-ultrviolet; PDA: photodiode array; NMR: nuclear magnetic resonance; FID: free induction decay; LC-MS: liquid chromatography-mass spectrometry; GC-MS: gas chromatography-mass spectrometry; HSQC: heteronuclear single quantum coherence; CPMG: Carr-Purcell-Meibum-Gill; VLDL: very low density lipoprotein; HDL: high density lipoprotein; EDTA: ethylenediaminetetraacetic acid; ANOVA: analysis of variance; AMIX: analysis of mixtures; SIMCA: soft independent modeling of class analogy; PCA: principal components analysis; OPLS-DA: orthogonal partial least-squares discriminant analysis; VIP: variable importance plot; AUROC: area under the receiver operating characteristic; TSP: sodium 3-(trimethylsilyl) propionate- 2,2,3,3- d4; DSA: deuterated 4-dimethyl-4-silapentane-1-ammonium trifluoroacetate; ESI: electrospray ionization; TCA: trichloroacetic acid; ACN: acetonitrile; dd H2O: distilled deionized water; FSH: follicle-stimulating hormone; LH: luteinizing hormone; OECD: Organisation for Economic Co-operation and Development.
The Effect of Teaching Formulaic Expressions Through Contrastive Lexical Approach on Iranian Pre-intermediate EFL Learners' Writing Skill

Ebrahimi F, Namaziandost E, Ziafar M, Ibna Seraj PM

J Psycholinguist Res, 2021 Oct;50(5):1087-1105.
PMID: 33830415 DOI: 10.1007/s10936-021-09778-z

This study aimed to investigate the effect of the contrastive lexical approach on Iranian EFL learners' writing skills. For this study, forty pre-intermediate students from a private English language institutes in Ahvaz, Iran were selected. Then, they were randomly divided into two equal groups of 20; one experimental and one control group. To have two groups of equal numbers, we used a block randomization sampling method. All of these students were female, ranging in age from 18 to 30. Their level of English language proficiency had already been determined by the Institute to be pre-intermediate. First, they were given a pre-test to determine their writing ability. Afterward, the experimental group received writing practices through the Contrastive Lexical Approach (CLA), during 14 sessions. Each session lasted for an hour and a half. The teacher sensitized learners in the experimental group towards the presence of L2 equivalents for L1 formulaic expressions, while the control group received an ordinary, traditional instruction, during which learners read texts containing the same formulaic expressions as for the experimental group without receiving any translation and were then asked to write about the same topics. At the end of the course, a post-test was administered to the two groups. Data were analyzed through independent and paired samples t tests after ensuring the normality of the data. Finally, to discover the power of the statistical tests, the effect size was also calculated. The study showed that using a contrastive lexical approach has a significant positive effect on Iranian EFL learners' writing skills. As the findings in this study propose, the writing skill can be improved through the use of a contrastive lexical approach. Teaching through a contrastive lexical approach, hopefully, gives the learners the chance to fathom their skillful writing competence, which requires the proper use of varied forms of structures and expressions and this, in turn, may sensitize them to know more about what language features to work on to increase their writing proficiency.
1HNMR-Based Discriminatory Analysis of Eurycoma longifolia from Different Locations and Establishing a Profile for Primary Metabolites Identification and Quassinoids Quantification

Ebrahimi F, Ibrahim B, Teh CH, Murugaiyah V, Lam CK

Planta Med, 2017 Jan;83(1-02):172-182.
PMID: 27399233 DOI: 10.1055/s-0042-110857

Quassinoids, the major secondary metabolites of Eurycoma longifolia roots, improve male fertility. Hence, it is crucial to investigate their quantitative level in E. longifolia extracts. A profile was established to identify the primary metabolites and major quassinoids, and quantify quassinoids using external calibration curves. Furthermore, the metabolic discrimination of E. longifolia roots from different regions was investigated. The (1)H-NMR spectra of the quassinoids, eurycomanone, eurycomanol, 13,21-dihydroeurycomanone, and eurycomanol-2-O-β-D-glycopyranoside were obtained. The (1)H-NMR profiles of E. longifolia root aqueous extracts from Perak (n = 30) were obtained and used to identify primary metabolites and the quassinoids. Selangor, Kedah, Terengganu (n = 5 for each), and Perak samples were checked for metabolic discrimination. Hotelling's T(2) plot was used to check for outliers. Orthogonal partial least-squares discriminant analysis was run to reveal the discriminatory metabolites. Perak samples contained formic, succinic, methylsuccinic, fumaric, lactic, acetic and syringic acids as well as choline, alanine, phenylalanine, tyrosine, α-glucose, eurycomanone, eurycomanol, 13,21-dihydroeurycomanone, and eurycomanol-2-O-β-D-glycopyranoside. The extracts from other locations contained the same metabolites. The limit of quantification values were 1.96 (eurycomanone), 15.62 (eurycomanol), 3.91 (13,21-dihydroeurycomanone), and 31.25 (eurycomanol-2-O-β-D-glycopyranoside) ppm. The Hotelling's T(2) plot revealed no outlier. The orthogonal partial least-squares discriminant analysis model showed that choline, eurycomanol, eurycomanol-2-O-β-D-glycopyranoside, and lactic and succinic acid levels were different among regions. Terengganu and Perak samples contained higher amounts of eurycomanol and eurycomanol-2-O-β-D-glycopyranoside, respectively. The current approach efficiently detected E. longifolia root metabolites, quantified the quassinoids, and discriminated E. longifolia roots from different locations. These findings could be applicable to future research on E. longifolia where the higher content of quassinoids is required.
Polar Quassinoids in Standardized Eurycoma longifolia Extract Formulated into a Lipid-Based Solid Dispersion to Improve Rat Sperm Count

Ma HQ, Ebrahimi F, Low BS, Khan NAK, Chan KL

Phytother Res, 2017 Dec;31(12):1875-1882.
PMID: 28948658 DOI: 10.1002/ptr.5930

Eurycoma longifolia Jack is popularly sought in Southeast Asian countries for traditional remedies to improve sexual performance and fertility. 13α(21)-Epoxyeurycomanone and eurycomanone, two major quassinoids in a root extract (TAF2) were reported to improve rat spermatogenesis and fertility. Unfortunately, these quassinoids possess low bioavailability because of high aqueous solubility and low lipid membrane permeability. Often, other possible barriers may be P-glycoprotein (P-gp) efflux in the gut and presystemic hepatic metabolism. The present study attempted to solve these problems by formulating a lipid-based solid dispersion (TAF2-SD) of optimized mixture of TAF2 and emulsifiers, which was then orally administered to rats prior to sperm count analysis. The TAF2-SD-treated rats showed significantly twofold (p
Fulltext Ethnographic Exploration of Empowerment to Improve Elderly Residents' Quality of Life

Tabatabaei SZ, Ebrahimi F, Hamzah ABH, Rezaeian M, Kamrani MA

Iran J Nurs Midwifery Res, 2017 Sep-Oct;22(5):414-419.
PMID: 29034000 DOI: 10.4103/ijnmr.IJNMR_70_16

BACKGROUND: Evidence underscores that empowerment is central to improve the elderly residents' quality of life. In truth, empowerment is a process through which individuals gain better control over their life. The aim of this study was to explore how perceived empowerment influence on the quality of life among elderly Malay residents.

MATERIALS AND METHODS: A focus ethnographic approach was employed in a Malaysian residential home between May 2011 and January 2012. Data were gathered from participant observations, field notes, in-depth interviews, and exploring related documents.

RESULTS: The analysis of the data gathered in the current study resulted in the development of three themes - social life and its requirements, caregivers' skills empowerment, and listening and supporting.

CONCLUSIONS: Findings of the study provide new insights that are useful in charting new guideline for care providers and policy makers to improve the elderly residents' quality of life.
Relationship between sociodemographics, dietary intake, and physical activity with gestational weight gain among pregnant women in Rafsanjan City, Iran

Ebrahimi F, Shariff ZM, Tabatabaei SZ, Fathollahi MS, Mun CY, Nazari M

J Health Popul Nutr, 2015 Mar;33(1):168-76.
PMID: 25995733

Gestational weight gain (GWG) is a determinant of health and nutrition of mothers and offspring. However, many factors associated with GWG are not completely understood. The present study assessed the relationship between sociodemographics, dietary intake, and physical activity with GWG in 308 Iranian pregnant women attending government healthcare centres in Rafsanjan city, Iran. Women gained an average of 12.87±3.57 kg during pregnancy while 54% did not gain weight within the Institute of Medicine (IOM)-recommended range. Univariate logistic models showed that gestaional weight gain was related to age, pre-pregnancy body mass index (BMI), energy intake, and sitting time. Cumulative logit model showed positive relationship between age (p=0.0137) and pre-pregnancy BMI (p<0.0001) with GWG. All pregnant women should be counselled on achieving the reccomended GWG to prevent adverse maternal and prenatal outcomes. Pre-pregnancy and gestational nutritional status and physical activity should be emphasized in antenatal care.