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  1. Venkateshbabu N, Anand S, Abarajithan M, Sheriff SO, Jacob PS, Sonia N
    Open Dent J, 2016;10:214-26.
    PMID: 27386007 DOI: 10.2174/1874210601610010214
    Complete eradication of microbial biofilms and elimination of the smear layer are the key factors during endodontic treatment. Various chemical irrigants have been proposed in the literature for the same. The major setback with these chemical irrigants is that they are not bio-friendly to the dental and peri-radicular tissues. In the recent years, research to use natural products for root canal disinfection has gained importance. The aim of this article is to compile various herbal products that have been used as an irrigants and intracanal medicaments in the field of Endodontics to eradicate the biofilm and remove smear layer.
  2. Nagendrababu V, Sheriff Sultan O, Kannathasan S, Patel AS, Chitra E, Neelakantan P, et al.
    Restor Dent Endod, 2018 Feb;43(1):e1.
    PMID: 29487832 DOI: 10.5395/rde.2018.43.e1
    Objectives: To determine the effect of root canal irrigants on the hydrophobicity and adherence ofStaphylococcus epidermidis(S. epidermidis) to root canal dentinin vitro.

    Materials and Methods: Root dentin blocks (n= 60) were randomly divided into 4 groups based on the irrigation regimen: group 1, saline; group 2, 5.25% sodium hypochlorite (NaOCl); group 3, 5.25% NaOCl followed by 17% ethylenediaminetetraacetic acid (EDTA); group 4, same as group 3 followed by 2% chlorhexidine (CHX). The hydrophobicity ofS. epidermidisto root dentin was calculated by cell surface hydrophobicity while the adherence was observed by fluorescence microscopy, and bacteria were quantified using ImageJ software (National Institutes of Health). Statistical analysis of the data was done using Kruskal-Wallis test and Mann-WhitneyUtest (p= 0.05).

    Results: The hydrophobicity and adherence ofS. epidermidisto dentin were significantly increased after irrigating with group 3 (NaOCl-EDTA) (p< 0.05), whereas in group 4 (NaOCl-EDTA-CHX) both hydrophobicity and adherence were significantly reduced (p< 0.05).

    Conclusions: The adherence ofS. epidermidisto dentin was influenced differently by root canal irrigants. Final irrigation with CHX reduces the bacterial adherence and may impact biofilm formation.

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