Displaying publications 1 - 20 of 32 in total

  1. Dondero TJ, Sivanandam S
    Med J Malaysia, 1973 Jun;27(4):306-9.
    PMID: 4270791
  2. Sivanandam S, Sandosham AA
    Med J Malaya, 1968 Mar;22(3):238.
    PMID: 4234713
  3. Afzan MY, Sivanandam S, Suresh K
    J Appl Microbiol, 2012 Jan;112(1):132-7.
    PMID: 22040369 DOI: 10.1111/j.1365-2672.2011.05185.x
    We previously reported that Modified Field Stain (MF) can be used as a rapid stain for diagnosis. In the present study we extend the observation to include the stain as an alternative method to assess viability of the cells.
  4. Afzan MY, Sivanandam S, Kumar GS
    Diagn Microbiol Infect Dis, 2010 Oct;68(2):159-62.
    PMID: 20846588 DOI: 10.1016/j.diagmicrobio.2010.06.005
    Trichomonas vaginalis, a flagellate protozoan parasite commonly found in the human genitourinary tract, is transmitted primarily by sexual intercourse. Diagnosis is usually by in vitro culture method and staining with Giemsa stain. There are laboratories that use Gram stain as well. We compared the use of modified Field's (MF), Giemsa, and Gram stains on 2 axenic and xenic isolates of T. vaginalis, respectively. Three smears from every sediment of spun cultures of all 4 isolates were stained, respectively, with each of the stains. We showed that MF staining, apart from being a rapid stain (20 s), confers sharper staining contrast, which differentiates the nucleus and the cytoplasm of the organism when compared to Giemsa and Gram staining especially on parasites from spiked urine samples. The alternative staining procedure offers in a diagnostic setting a rapid stain that can easily visualize the parasite with sharp contrasting characteristics between organelles especially the nucleus and cytoplasm. Vacuoles are more clearly visible in parasites stained with MF than when stained with Giemsa.
  5. Sivanandam S, Mak JW, Lai PF
    PMID: 1145240
    R. sabanus and R. muelleri are very common in the lowland forests of Malaysia. In nature they are infected with Breinlia sp. and D. ramachandrani. In an attempt to determine whether they are also susceptible to subperiodic B. malayi and thereby being potential reservoirs of infection of the disease, 24 R. muelleri and 17 R. sabanus were experimentally infected with the parasite. Results show that although they can support the full development of the parasite, they are poor hosts. This confirms the observation that in Malaysia natural infection of Rattus spp. with the parasite has not been seen. These rats therefore are probably not important in the zoonotic transmission of subperiodic B. malayi in Malaysia.
  6. Sandosham AA, Sivanandam S, Fong YL, Omar I
    Med J Malaya, 1966 Jun;20(4):340.
    PMID: 4224353
  7. Ramachandran CP, Sandosham AA, Sivanandam S
    Med J Malaya, 1966 Jun;20(4):333.
    PMID: 4224348
  8. Muslim A, Fong MY, Mahmud R, Sivanandam S
    Trop Biomed, 2013 Dec;30(4):727-30.
    PMID: 24522144 MyJurnal
    A case of human eye infection caused by Brugia pahangi was reported in 2010 in a semi rural village in Selangor, peninsular Malaysia. Our report here reveals results of investigation on the vector and animal host for the transmission of the infection. We conducted entomological survey and cat blood examination in the vicinity of the patient's home. The mosquito species Armigeres subalbatus was incriminated as the vector, whereas cat served as the reservoir host.
  9. Chandramathi S, Suresh K, Sivanandam S, Kuppusamy UR
    PLoS One, 2014;9(5):e94567.
    PMID: 24788756 DOI: 10.1371/journal.pone.0094567
    Stress alters the oxidant-antioxidant state and immune cell responses which disrupts its function to combat infection. Blastocystis hominis, a common intestinal protozoan has been reported to be opportunistic in immunocompromised patients namely cancer. B. hominis infectivity in other altered immune system conditions especially stress is unknown. We aimed to demonstrate the stress effects towards the susceptibility and pathogenicity of B. hominis infection.
  10. Muslim A, Fong MY, Mahmud R, Lau YL, Sivanandam S
    Parasit Vectors, 2013;6:219.
    PMID: 23898840 DOI: 10.1186/1756-3305-6-219
    In 2011, we reported occurrence of natural human infections with Brugia pahangi, a filarial worm of dogs and cats, in a surburb of Kuala Lumpur, the capital city of Malaysia. Our preliminary entomological survey at that time suggested the mosquito species Armigeres subalbatus as the vector of the zoonotic infections. In this present report, we provide biological evidence to confirm our preliminary finding.
  11. Pirehma M, Suresh K, Sivanandam S, Anuar AK, Ramakrishnan K, Kumar GS
    Parasitol Res, 1999 Oct;85(10):791-3.
    PMID: 10494803
    Acanthamoeba sp. is a free-living amoeba known to cause chronic central nervous system infection or eye infection in humans. Many cases remain undetected for want of a good detection system. We report for the first time a rapid staining method to facilitate the identification of Acanthamoeba sp. using the modified Field's staining technique. A. castellanii, which was used in the present experiment, is maintained in our laboratory in mycological peptone medium (Gibco). The cultures were pooled together and smears were made on glass slides for staining purposes. Different types of stains such as Field's stain, modified Field's stain, Wright's stain, Giemsa stain, Ziehl-Neelsen stain, and trichrome stain were used to determine the best stain for the identification of this amoeba. The concentration of various stains and the duration of staining were varied to provide the best color and contrast for each stain. Acanthamoeba was also obtained from the brain of experimentally infected mice and was stained with various stains as mentioned above to determine the best stain for use in identifying the presence of this parasite in experimentally infected animals. The modified Field's stain gives a very good color contrast as compared with other stains. Furthermore, it takes only 20 s to be carried out using the least number of reagents, making it suitable for both laboratory and field use.
  12. Ramachandran CP, Dondero TJ, Mullin SW, Sivanandam S, Stevens S
    Med J Malaya, 1971 Jun;25(4):273-7.
    PMID: 4261299
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