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The impact of Japanese nuclear wastewater discharge into the sea on the global economy: Input-output model approach

Wu X, Zhang Y, Feng X

Mar Pollut Bull, 2023 Jul;192:115067.
PMID: 37269704 DOI: 10.1016/j.marpolbul.2023.115067

As the division of work within the world economic system becomes increasingly complex, the impact of disturbing events on the economic system is expanding. Recently, Japan proposed to discharge nuclear wastewater into the Pacific Ocean, which will cause damage to marine fisheries, thereby seriously affecting fisheries and other industries in Japan and other countries and regions around the world. Considering different scenarios of final and intermediate demand shifting, this paper uses the Inoperability Input-Output Model (IIM) and Multi-Region Input-Output Model (MRIO) to simulate the economic consequences of nuclear wastewater discharge in Japan and calculate the economic changes of each industry and country (region). The results show that: In the short term, when only the final demand for Japanese fishery products decreases. (1) The ten countries (regions) with significant economic losses are Japan, the United States, Chinese Taipei, Canada, Chile, South Africa, Mexico, Peru, the United Kingdom, and Ireland. (2) The ten countries (regions) with a significant increase in total output due to demand shift are China (People's Republic of), the Rest of the World, India, Indonesia, Viet Nam, the Philippines, Brazil, Myanmar, the Russian Federation, and Malaysia. (3) A ranking of changes in the total output of different industries. In the long term, when both intermediate and final demand for Japanese fishery products decrease. (4) The change in value added in Japan. (5) The change in value added of 67 countries (regions) worldwide. The ten countries (regions) with the most significant increase in value-added are the Russian Federation, China (People's Republic of), the Rest of the World, the United States, Indonesia, Australia, Norway, Korea, Viet Nam, and Myanmar. The ten countries (regions) with the most significant decrease in value-added are Japan, Chinese Taipei, Chile, South Africa, Peru, Thailand, Mexico, Cambodia, Costa Rica, and Morocco. Changes in value added of 45 industrial sectors worldwide.
Fulltext Student Behavior Recognition From Heterogeneous View Perception in Class Based on 3-D Multiscale Residual Dense Network for the Analysis of Case Teaching

Liu H, Liu Y, Zhang R, Wu X

Front Neurorobot, 2021;15:675827.
PMID: 34393749 DOI: 10.3389/fnbot.2021.675827

The study of student behavior analysis in class plays a key role in teaching and educational reforms that can help the university to find an effective way to improve students' learning efficiency and innovation ability. It is also one of the effective ways to cultivate innovative talents. The traditional behavior recognition methods have many disadvantages, such as poor robustness and low efficiency. From a heterogeneous view perception point of view, it introduces the students' behavior recognition. Therefore, we propose a 3-D multiscale residual dense network from heterogeneous view perception for analysis of student behavior recognition in class. First, the proposed method adopts 3-D multiscale residual dense blocks as the basic module of the network, and the module extracts the hierarchical features of students' behavior through the densely connected convolutional layer. Second, the local dense feature of student behavior is to learn adaptively. Third, the residual connection module is used to improve the training efficiency. Finally, experimental results show that the proposed algorithm has good robustness and transfer learning ability compared with the state-of-the-art behavior recognition algorithms, and it can effectively handle multiple video behavior recognition tasks. The design of an intelligent human behavior recognition algorithm has great practical significance to analyze the learning and teaching of students in the class.
Construction of DNA nanotubes with controllable diameters and patterns using hierarchical DNA sub-tiles

Shi X, Wu X, Song T, Li X

Nanoscale, 2016 Jul 22.
PMID: 27444699

The design of DNA nanotubes is a promising and hot research branch in structural DNA nanotechnology, which is rapidly developing as a versatile method for achieving subtle nanometer scale materials and molecular diagnostic/curative devices. Multifarious methods have been proposed to achieve varied DNA nanotubes, such as using square tiles and single-stranded tiles, but it is still a challenge to develop a bottom-up assembly way to build DNA nanotubes with different diameters and patterns using certain universal DNA nanostructures. This work addresses the challenge by assembling three types of spatial DNA nanotubes with different diameters and patterns from the so-called "basic bricks", i.e., hierarchical DNA sub-tiles. A high processing rate and throughput synthesis of DNA nanotubes are observed and analyzed by atomic force microscopy. Experimental observations and data analysis suggests the stability and controllability of DNA nanotubes assembled by hierarchical DNA sub-tiles.
Fulltext Addressing the COVID-19 Shock: The Potential Job Creation in China by the RCEP

Wu X, Yong CC, Lee ST

Int J Environ Res Public Health, 2022 Nov 25;19(23).
PMID: 36497744 DOI: 10.3390/ijerph192315669

In 2020, coronavirus disease (COVID-19) left around 81% of the global workforce, nearly 2.7 billion workers, affected. Employment in China was the first to be hit by COVID-19. The Regional Comprehensive Economic Partnership (RCEP) is expected to bring dynamism to China's employment market in an era of long COVID-19. This study aims to examine the number of sectoral jobs that the RCEP will create in China, with the number of skilled or unskilled labour employed in each sector. The exogenous shocks to the RCEP can be reflected in the number of jobs created through multipliers based on a social accounting matrix compiled from China's input-output tables in 2017, combined with the employment satellite accounts compiled. The results show that the RCEP is expected to create over 17 million potential jobs in China, with unskilled labour accounting for 10.44 million and skilled labour for 6.77 million. It is even expected that there will be job losses in the metalworking machinery sector. The contribution of this paper can serve as a reference for policies to protect vulnerable sectors, further open up trade markets and strengthen cooperation among RCEP members as important measures to address the employment impact of long COVID-19.
Fulltext Motivational Processes Influencing Mental Health Among Winter Sports Athletes in China

Wu X, Zainal Abidin NE, Aga Mohd Jaladin R

Front Psychol, 2021;12:726072.
PMID: 34603145 DOI: 10.3389/fpsyg.2021.726072

This study examined the association between motivational processes, psychological distress (depression, anxiety, and stress), and burnout among winter sports athletes within the Hierarchical Model of Intrinsic and Extrinsic Motivation (HMIEM). A total of 685 winter sport athletes participated in this study (377 males, 308 females, age range 18-25 years), from three sport universities across nine winter sports. They completed five psychometric inventories related to motivational factors and mental disorders. Overall, a task-oriented climate showed a positive association with basic psychological needs, eliciting a positive pathway to autonomous and controlled motivation. In contrast, an ego-oriented climate showed a negative association with basic psychological needs, eliciting a negative pathway to amotivation. Autonomous and controlled motivation were negatively associated with symptoms of psychological distress and burnout, while amotivation was positively associated with symptoms of psychological distress and burnout. These findings highlight the complex relationships between various motivational factors and mental health disorders among winter sport athletes, and support the essential requirement for adding mental health factors to the outcomes of the HMIEM sequence.
Fulltext Discernment on assistive technology for the care and support requirements of older adults and differently-abled individuals

Muthu P, Tan Y, Latha S, Dhanalakshmi S, Lai KW, Wu X

Front Public Health, 2022;10:1030656.
PMID: 36699937 DOI: 10.3389/fpubh.2022.1030656

Assistive technology for the differently abled and older adults has made remarkable achievements in providing rehabilitative, adaptive, and assistive devices. It provides huge assistance for people with physical impairments to lead a better self-reliant daily life, in terms of mobility, education, rehabilitation, etc. This technology ranges from simple hand-held devices to complex robotic accessories which promote the individual's independence. This study aimed at identifying the assistance required by differently-abled individuals, and the solutions proposed by different researchers, and reviewed their merits and demerits. It provides a detailed discussion on the state of art assistive technologies, their applications, challenges, types, and their usage for rehabilitation. The study also identifies different unexplored research areas related to assistive technology that can improve the daily life of individuals and advance the field. Despite their high usage, assistive technologies have some limitations which have been briefly described in the study. This review, therefore, can help understand the utilization, and pros and cons of assistive devices in rehabilitation engineering and assistive technologies.
Case Report: Cord blood-derived natural killer cells as new potential immunotherapy drug for solid tumor: a case study for endometrial cancer

Mu Y, Tong J, Wang Y, Yang Y, Wu X

Front Immunol, 2023;14:1213161.
PMID: 37457710 DOI: 10.3389/fimmu.2023.1213161

Adoptive transfer of natural killer (NK) cells represents a viable treatment method for patients with advanced malignancies. Our team previously developed a simple, safe, and cost-effective method for obtaining high yields of pure and functional NK cells from cord blood (CB) without the need for cell sorting, feeder cells, or multiple cytokines. We present the case of a 52-year-old female patient diagnosed with poorly differentiated stage IVB (T3N2M1) endometrial cancer, who exhibited leukemoid reaction and pretreatment thrombocytosis as paraneoplastic syndromes. The patient received two courses of CB-derived NK (CB-NK) cell immunotherapy between March and September 2022, due to her extremely low NK cell activity. Two available CB units matched at 8/10 HLA with KIR-mismatch were chosen, and we were able to produce NK cells with high yield (>1.0×1010 NK cells), purity (>90%), and function (>80%) from CB without cell sorting, feeder cells, or multiple cytokines. These cells were then adoptively transferred to the patient. No adverse effects or graft-versus-host disease were observed after infusion of CB-NK cells. Our clinical experience supports the efficacy of CB-NK cell treatment in increasing NK cell activity, depleting tumor activity, improving quality of life, and reducing the size of abdominal and pelvic masses with the disappearance of multiple lymph node metastases through the regulation of systemic antitumor immunity. Remarkably, the white blood cell and platelet counts decreased to normal levels after CB-NK cell immunotherapy. This clinical work suggests that CB-NK cell immunotherapy holds promise as a therapeutic approach for endometrial cancer.
Current advances in the development of meniscus tissue engineering: narrative review

Tong JB, Sanjiv R, Elderdery A, Wu X, Rajesh R, Suresh Kumar S, et al.

Med J Malaysia, 2023 Jul;78(4):534-540.
PMID: 37518929

INTRODUCTION: The meniscus plays an important role in maintaining homeostasis to facilitate the normal function of the knee joint. It is one of the most commonly injured areas of the knee joint. Meniscal-related injuries can lead to significantly decreased athletic ability, and their incidence has increased yearly. It has been found that most meniscal injuries are irreparable, and meniscectomy can increase the predisposition to knee osteoarthritis. Tissue engineering technology on meniscus repairing and transplantation has received widespread attention recently. This review aimed to analyse the scientific literature regarding the potential applications of tissue engineering on meniscus repairing and transplantation procedures.
METHOD AND MATERIALS: The electronic search was carried out using PubMed/MEDLINEⓇdatabases with the keywords "tissue engineering AND meniscus" spanning the period of publications from Jan 1980 until Dec 2022.
RESULTS: The literature search identified 405 references in PubMed/MEDLINE, and 179 were selected following the eligibility requirements. The research analysis showed that the existing meniscal tissue engineering studies used a wide variety of seed cells, cytokines, bioactive materials and 3D structures. Each showed distinct advantages and disadvantages in terms of biocompatibility, degradability, mechanical strength, porosity, and etc. It was noted that 3D printing technology is promising for tissue engineering meniscus research. In addition, the optimal use of compression and hydrostatic pressure to markedly improve the functional properties of tissue-engineering meniscal can serve as an useful strategy.
CONCLUSION: This review analysed the different approaches employed for meniscus tissue engineering and regeneration. Meniscal tissue engineering still faces several major challenges in terms of seed cells, choice of materials and 3D printing strategies, which should be effectively overcome to harness the full potential of this technology.
Fulltext Ultrasonic liquid exfoliation for producing graphene materials from rice stem: Investigating cellular components and functionalities

Wu X, Sivakumar M, Lim SS, Wu T, Heng PC

Ultrason Sonochem, 2024 Feb;103:106782.
PMID: 38309050 DOI: 10.1016/j.ultsonch.2024.106782

This study investigates a prospective and straightforward method for producing graphene material derived from biomass, examining the influence of plant cell composition and functions. The experimental outcomes highlight ultrasound's crucial role in synthesizing graphene material sourced from biomass. Ultrasound, a pivotal element in the experiment, significantly affects graphene production from biomass by working synergistically with the liquid components in the solvent system. Notably, the ethanol content reduces the solution's surface tension, facilitating the effective dispersion of biochar and graphene oxide sheets throughout the process. Simultaneously, the water content maintains the solution's polarity, enhancing the cavitation effect induced by ultrasound. Biomass-derived graphene is exfoliated utilizing an ultrasonic bath system (134.4 W, 40 kHz, 0.5 W/cm2) from biochar. The as-synthesized graphene oxide exhibits a structure comprising a few layers while remaining intact, featuring abundant functional groups. Interestingly, the resulting product displays nanopores with an approximate diameter of 100 nm. These nanopores are attributed to preserving specific cell structures, particularly those with specialized cell wall structures or secondary metabolite deposits from biomass resources. The study's findings shed light on the impact of cellular structure on synthesizing graphene material sourced from biomass, emphasizing the potential application of ultrasound as a promising approach in graphene production.
Fulltext Significant Broadband Photocurrent Enhancement by Au-CZTS Core-Shell Nanostructured Photocathodes

Zhang X, Wu X, Centeno A, Ryan MP, Alford NM, Riley DJ, et al.

Sci Rep, 2016;6:23364.
PMID: 26997140 DOI: 10.1038/srep23364

Copper zinc tin sulfide (CZTS) is a promising material for harvesting solar energy due to its abundance and non-toxicity. However, its poor performance hinders their wide application. In this paper gold (Au) nanoparticles are successfully incorporated into CZTS to form Au@CZTS core-shell nanostructures. The photocathode of Au@CZTS nanostructures exhibits enhanced optical absorption characteristics and improved incident photon-to-current efficiency (IPCE) performance. It is demonstrated that using this photocathode there is a significant increase of the power conversion efficiency (PCE) of a photoelectrochemical solar cell of 100% compared to using a CZTS without Au core. More importantly, the PCE of Au@CZTS photocathode improved by 15.8% compared to standard platinum (Pt) counter electrode. The increased efficiency is attributed to plasmon resonance energy transfer (PRET) between the Au nanoparticle core and the CZTS shell at wavelengths shorter than the localized surface plasmon resonance (LSPR) peak of the Au and the semiconductor bandgap.
Characteristics and mechanisms of phosphorous adsorption by rape straw-derived biochar functionalized with calcium from eggshell

Cao H, Wu X, Syed-Hassan SSA, Zhang S, Mood SH, Milan YJ, et al.

Bioresour Technol, 2020 Dec;318:124063.
PMID: 32905948 DOI: 10.1016/j.biortech.2020.124063

Biochar modified with calcium source is acted as an effective adsorbent for phosphorous recovery. In this research, eggshell is used as a low-cost and environmentally friendly calcium source to replace chemical reagents such as CaCO3, Ca(OH)2 and CaCl2 used in the modified biochar production. Biochar derived from rape straw and modified with eggshell shows prominent phosphorous adsorption performance (e.g., equilibrium adsorption amount, 109.7 mg/g). The kinetic and isotherm analysis demonstrate that chemical adsorption process is performed as the main controlled step for the modified biochar adsorption, and the phosphate adsorption process is composed of both monolayer adsorption and multi-layer adsorption. Moreover, it is found from the physicochemical structures comparison before and after phosphate adsorption that Ca-P precipitation, hydrogen bonding and electrostatic attraction are identified as main adsorption mechanisms. In addition, the adsorbed phosphates are mainly distributed inside the space with pore sizes of 15-50 nm.
Reply to "Meta-analysis of astaxanthin supplementation on obesity, blood pressure, CRP, glycemic biomarkers, and lipid profile: Reanalysis is needed"

Xia W, Tang N, Kord-Varkaneh H, Low TY, Tan SC, Wu X, et al.

Pharmacol Res, 2021 01;163:105167.
PMID: 32860944 DOI: 10.1016/j.phrs.2020.105167
Evaluation of biophysical as well as biochemical potential of curcumin and resveratrol during prostate cancer

Guo W, Wu X, Li Y, Gao J, Wang F, Jin Y, et al.

J Drug Target, 2020 01;28(1):41-45.
PMID: 30943812 DOI: 10.1080/1061186X.2019.1601199

Purpose: The present study evaluated biochemical as well as biophysical mechanisms behind the synergistic effects of curcumin and resveratrol during prostate carcinogenesis.Methods: The rats were segregated into five groups that included normal control, 3,2'-dimethyl-4-aminobiphenyl (DMAB)treated, DMAB + curcumin treated, DMAB + resveratrol-treated and DMAB + curcumin + resveratrol-treated.Results: The DMAB treatment resulted in a significant increase in the levels of lipid peroxidation (LPO) in DMAB treated rats. Also, significant changes were recorded in the enzyme activities of both drug metabolising enzyme and antioxidant enzymes after DMAB treatment. Further, radiorespirometric studies showed a significant increase in the 14C-glucose turnover as well as 14C-glucose uptake in the prostate slices of DMAB treated rats. Moreover, a significant rise in cell proliferation was confirmed indirectly by enhanced uptake of 3H-thymidine in the prostate slices of DMAB treated rats. Interestingly, combined treatment of curcumin and resveratrol to DMAB treated animals resulted in a significant decrease in lipid peroxidation, 14C glucose uptakes/turnover and 3H-thymidine uptake in the DMAB treated rats. Besides this, curcumin and resveratrol in combination significantly modulated biochemical indices including drug-metabolising enzymes; antioxidant enzymes in DMBA treated rats.Conclusion: The study, therefore, concludes that the combination of curcumin and resveratrol holds strong modulatory potential against prostate carcinogenesis.
The effects of astaxanthin supplementation on obesity, blood pressure, CRP, glycemic biomarkers, and lipid profile: A meta-analysis of randomized controlled trials

Xia W, Tang N, Kord-Varkaneh H, Low TY, Tan SC, Wu X, et al.

Pharmacol Res, 2020 11;161:105113.
PMID: 32755613 DOI: 10.1016/j.phrs.2020.105113

BACKGROUND AND AIM: Previous studies lack consistent conclusions as to whether astaxanthin is actually linked to various health benefits as claimed. Here, we attempt to unravel the association of astaxanthin consumption with selected health benefits by performing a systematic review and meta-analysis.
METHODS: Online literature search databases including Scopus, Web of Science, PubMed/Medline, Embase and Google Scholar were searched to discover relevant articles available up to 17 March 2020. We used mean changes and SD of the outcomes to assess treatment response from baseline and mean difference, and 95 % CI were calculated to combined data and assessment effect sizes in astaxanthin and control groups.
RESULTS: 14 eligible articles were included in the final quantitative analysis. Current study revealed that astaxanthin consumption was not associated with FBS, HbA1c, TC, LDL-C, TG, BMI, BW, DBP, and SBP. We did observe an overall increase in HDL-C (WMD: 1.473 mg/dl, 95 % CI: 0.319-2.627, p = 0.012). As for the levels of CRP, only when astaxanthin was administered (i) for relatively long periods (≥ 12 weeks) (WMD: -0.528 mg/l, 95 % CI: -0.990 to -0.066), and (ii) at high dose (> 12 mg/day) (WMD: -0.389 mg/dl, 95 % CI: -0.596 to -0.183), the levels of CRP would decrease.
CONCLUSION: In summary, our systematic review and meta-analysis revealed that astaxanthin consumption was associated with increase in HDL-C and decrease in CRP. Significant associations were not observed for other outcomes.
Evaluation of the inhibitory effects of drugs on the growth of Babesia gibsoni using relative quantification real-time PCR

He WH, Feng XX, Wu X, Zhai XH, Li YY, Zhang B, et al.

Trop Biomed, 2020 Dec 01;37(4):871-876.
PMID: 33612740 DOI: 10.47665/tb.37.4.871

To evaluate the inhibitory effects of drugs on the growth of Babesia gibsoni, relative quantification real-time PCR method was developed in this study. The 18S rRNA gene was used as a target gene for the 2-ΔΔCt method analysis. Additionally, chicken RNA was added to the parasitized blood before total RNA extraction. The chicken β-actin gene was selected as an internal control gene for the 2-ΔΔCt method analysis. The 100 µL parasitized blood samples with different percentages of parasitized erythrocytes (PPEs) (3%, 1.5%, 0.75%, 0.375% and 0.1875%) were prepared for relative quantification of B. gibsoni. Regression analysis results revealed significant linear relationships between the relative quantification value and parasitemia. 18S rRNA gene expression was significantly decreased after treatment with diminazene aceturate and artesunate in vitro drug sensitivity test. This result suggested that this relative quantification real-time PCR method can be used to evaluate the effects of drug inhibition.
Fulltext Therapeutic Potential of Bama Pig Adipose-Derived Mesenchymal Stem Cells for the Treatment of Carbon Tetrachloride-Induced Liver Fibrosis

Wu X, Zhang S, Lai J, Lu H, Sun Y, Guan W

Exp Clin Transplant, 2020 12;18(7):823-831.
PMID: 33349209 DOI: 10.6002/ect.2020.0108

OBJECTIVES: Liver fibrosis is inevitable in the healing process of liver injury. Liver fibrosis will develop into liver cirrhosis unless the damaging factors are removed. This study investigated the potential therapy of Bama pig adipose-derived mesenchymal stem cells in a carbon tetrachloride-induced liver fibrosis Institute of Cancer Research strain mice model.
MATERIALS AND METHODS: Adipose-derived mesenchymal stem cells were injected intravenously into the tails of mice of the Institute of Cancer Research strain that had been treated with carbon tetrachloride for 4 weeks. Survival rate, migration, and proliferation of adipose-derived mesenchymal stem cells in the liver were observed by histochemistry, fluorescent labeling, and serological detection.
RESULTS: At 1, 2, and 3 weeks after adipose-derived mesenchymal stem cell injection, liver fibrosis was significantly ameliorated. The injected adipose-derived mesenchymal stem cells had hepatic differentiation potential in vivo, and the survival rate of adipose-derived mesenchymal stem cells declined over time.
CONCLUSIONS: The findings in this study confirmed that adipose-derived mesenchymal stem cells derived from the Bama pig can be used in the treatment of liver fibrosis, and the grafted adipose-derived mesenchy-mal stem cells can migrate, survive, and differentiate into hepatic cells in vivo.
Fulltext Testing role of green financing on climate change mitigation: Evidences from G7 and E7 countries

Wu X, Sadiq M, Chien F, Ngo QT, Nguyen AT, Trinh TT

Environ Sci Pollut Res Int, 2021 Dec;28(47):66736-66750.
PMID: 34235703 DOI: 10.1007/s11356-021-15023-w

The study estimates the long-run dynamics of a cleaner environment in promoting the gross domestic product of E7 and G7 countries. The recent study intends to estimate the climate change mitigation factor for a cleaner environment with the GDP of E7 countries and G7 countries from 2010 to 2018. For long-run estimation, second-generation panel data techniques including augmented Dickey-Fuller (ADF), Phillip-Peron technique and fully modified ordinary least square (FMOLS) techniques are applied to draw the long-run inference. The results of the study are robust with VECM technique. The outcomes of the study revealed that climate change mitigation indicators significantly affect the GDP of G7 countries than that of E7 countries. The GDP of both E7 and G7 countries is found depleting due to less clean environment. However, green financing techniques helps to clean the environment and reinforce the confidence of policymakers on the elevation of green economic growth in G7 and E7 countries. Furthermore, study results shown that a 1% rise in green financing index improves the environmental quality by 0.375% in G7 countries, while it purifies 0.3920% environment in E7 countries. There is a need to reduce environmental pollution, shift energy generation sources towards alternative, innovative and green sources.The study also provides different policy implications for the stakeholders guiding to actively promote financial hedging for green financing. So that climate change and envoirnmental pollution reduction could be achieved effectively. The novelty of the study lies in study framework.
TMT-labeled quantitative proteomic reveals the mechanism of proteolysis and taste improvement of dry-cured bacon with Staphylococcus co-inoculation

Zhou C, Wu X, Pan D, Xia Q, Sun Y, Geng F, et al.

Food Chem, 2024 Mar 15;436:137711.
PMID: 37839122 DOI: 10.1016/j.foodchem.2023.137711

To understand the mechanism of co-inoculation of Staphylococcus xylosus and Staphylococcus vitulinus (SX & SV) on structural protein degradation and taste enhancement of dry-cured bacon, protease activities, protein degradation, surface morphology of proteins and taste parameters of dry-cured bacon with Staphylococcus inoculation were investigated. The dry-cured bacon with co-inoculation of Staphylococcus xylosus and Staphylococcus vitulinus showed the best taste attributes. High residual activities in cathepsin B + L (more than 1.6-fold) and alanyl aminopeptidase (more than 1.4-fold) accelerated structural protein degradation in SX & SV. 32 down-regulated proteins were identified in SX & SV by TMT-labeled quantitative proteomic compared with control group; myosin and actin showed the most intense response to the accumulation of sweet and umami amino acids, and atomic force microscopy confirmed structural proteins breakdown by morphological changes. The accumulation of glutamic acid, alanine and lysine was mainly responsible for taste improvement of dry-cured bacon with Staphylococcus co-inoculation.
Expression pattern of AGPATs isoforms indicate different functions during the triacylglyceride synthesis in Chinese mitten crab, Eriocheir sinensis

Pan K, Zhu B, Wang L, Guo Q, Shu-Chien AC, Wu X

Comp Biochem Physiol A Mol Integr Physiol, 2024 Jan;287:111535.
PMID: 37852318 DOI: 10.1016/j.cbpa.2023.111535

The 1-acylglycerol-3-phosphate acyltransferase (AGPAT) acts as a crucial enzyme in the process of triacylglycerol (TAG) synthesis, enabling the acylation of lysophosphatidic acid (LPA) into phosphatidic acid (PA). In order to decode the distinctive roles of AGPAT isoforms in the TAG production pathway, three AGPAT isoforms were detected for the first time in the Chinese mitten crab Eriocheir sinensis (Es-agpat2, Es-agpat3, and Es-agpat4). The mRNA levels of Es-agpat2 and Es-agpat4 demonstrated a conspicuous presence in the hepatopancreas, with subsequent high levels in the heart, muscle, and thoracic ganglion. On the other hand, the thoracic ganglion exhibited abundant levels of Es-agpat3, while other tissues recorded relatively low expression levels. Observing the molting cycle of E. sinensis, the hepatopancreas showed minimum expression levels of Es-agpat2 and Es-agpat4 at stage A/B. A peak at stage C was noted, which was then followed by a gradual drop until stage E. For the ovarian development cycle, stage II witnessed the maximum expression level of Es-agpat2 and Es-agpat4, succeeded by a sharp fall in stage III. After this, there was an increasing trend from stage III up to stage V. Expression of Es-agpat3 in the hepatopancreas was consistently lower than Es-agpat2 and Es-agpat4 during either the molting or ovarian development. However, in terms of ovarian expression, Es-agpat3 outperformed Es-agpat2 and Es-agpat4. It exhibited a steep increase in expression, peaking at stage II and subsequently diminishing. In situ hybridization (ISH) revealed that in stages II and IV hepatopancreas, Es-agpat4-mRNA was primarily located in fibrillar cells (F cell) and resorptive cells (R cell), with no signal from Es-agpat3. During stage II of ovarian development, both Es-agpat3-mRNA and Es-agpat4-mRNA were located in the cytoplasm of previtellogenic oocyte (PRO) and endogenous vitellogenic oocyte (EN), with no expression at stage IV. Additionally, the silencing of Es-agpat2 and Es-agpat4 caused a downward trend in the expression levels of all subsequent genes in the E. sinensis TAG synthesis pathway. To sum up, these findings suggest that the three Es-agpats may have unique functions in TAG synthesis during either the molting process or ovarian maturation of E. sinensis.
Functional transcriptome reveals hepatopancreatic lipid metabolism during the molting cycle of the Chinese mitten crab Eriocheir sinensis

Li Z, Zhang G, Pan K, Niu X, Shu-Chien AC, Chen T, et al.

Comp Biochem Physiol A Mol Integr Physiol, 2023 Oct;284:111474.
PMID: 37406959 DOI: 10.1016/j.cbpa.2023.111474

Crustacean molting is highly related to energy and lipid metabolism. This study was conducted to detect the changes of total lipids (TL), triacylglyceride (TAG), phospholipid (PL) and lipid droplets in hepatopancreas, and then to investigate the gene expression patterns related to hepatopancreatic lipid metabolism during the molting cycle of Chinese mitten crab Eriocheir sinensis. Hepatopancreatic TL and TAG increased significantly from post-molt stage to pre-molt stage, then decreased significantly from pre-molt stage to ecdysis stage, which is consistent to the changes of neutral lipid-rich adipocytes in hepatopancreas. By transcriptomic analysis, 65,325 transcripts were sequenced and assembled, and 28,033 transcripts were annotated. Most genes were related to energy metabolism, and the enriched genes were involved in carbohydrate and lipid metabolism and biosynthesis, especially in de novo synthesis of fatty acids and TAG, and ketone body production. Compared to the inter-molt stages, acetyl-CoA carboxylase, fatty acid synthase and other genes related to the synthesis of fatty acids were upregulated in the pre-molt stage. TAG synthesis related genes, including Glycerol-3-phosphate acyltransferase and 1-acylglycerol-3-phosphate acyltransferases, were upregulated in the post-molt stage compared to the inter-molt stage. The expression of ketone body-related genes had no significant changes during the molting cycle. Compared to the TAG synthetic pathway, ketone body biosynthesis may contribute less/secondarily to fatty acid metabolic processes, which could be involved in the other physiological processes or metabolism. In conclusion, these results showed that TAG is the major lipid deposition during inter- and pre-molt stages, and the most genes are related to the fatty acids and TAG metabolism in the hepatopancreas during the molting cycle of E. sinensis.

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