Displaying publications 1 - 20 of 102 in total

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  1. Lee SH, H'ng PS, Peng TL, Lum WC
    Pak. J. Biol. Sci., 2013 Nov 1;16(21):1415-8.
    PMID: 24511759
    The objective of this study is to investigate the feasibility of formaldehyde catcher as termites repellent. Single-layered UF-bonded particleboard was post-treated with formaldehyde catcher and heat respectively. Besides that, some boards were also produced with the formaldehyde catcher was added into the resin during the blending process, called add-in method. Particleboard post-treated with formaldehyde catcher reported the most severe attack. Heat-treated particleboard showed slightly better durability than the control blocks while the add-in catcher showed the best durability among three methods. A valid test was obtained as the termites survived the first week of the test. However, all the termites were found dead at the end of the test.
    Matched MeSH terms: Formaldehyde*
  2. Edwin Shiaw CS, Shiran MS, Cheah YK, Tan GC, Sabariah AR
    Med. J. Malaysia, 2010 Jun;65(2):133-7.
    PMID: 23756798 MyJurnal
    This study was done to evaluate various DNA and RNA extractions from archival FFPE tissues. A total of 30 FFPE blocks from the years of 2004 to 2006 were assessed with each modified and adapted method. Extraction protocols evaluated include the modified enzymatic extraction method (Method A), Chelex-100 extraction method (Method B), heat-induced retrieval in alkaline solution extraction method (Methods C and D) and one commercial FFPE DNA Extraction kit (Qiagen, Crawley, UK). For RNA extraction, 2 extraction protocols were evaluated including the enzymatic extraction method (Method 1), and Chelex-100 RNA extraction method (Method 2). Results show that the modified enzymatic extraction method (Method A) is an efficient DNA extraction protocol, while for RNA extraction, the enzymatic method (Method 1) and the Chelex-100 RNA extraction method (Method 2) are equally efficient RNA extraction protocols.
    Matched MeSH terms: Formaldehyde
  3. Hartini Yusof, Mohamed Kamel Abd Ghani
    MyJurnal
    Infeksi Entamoeba histolytica adalah tersebar di seluruh dunia dengan prevalens infeksi yang lebih tinggi di kalangan masyarakat terpinggir termasuk Orang Asli yang tinggal di kawasan tropika dan subtropika. Seramai 71 orang kanak-kanak Orang Asli dari Pos Lenjang, Pahang telah terlibat di dalam kajian ini. Bagi kajian yang lebih terperinci, kumpulan kanak-kanak ini telah dibahagikan menurut jantina dan umur. Sampel feses dikumpul dan setiap sampel diperiksa bagi pengenalpastian Entamoeba histolytica dengan menggunakan 3 jenis teknik diagnostik yang berbeza iaitu teknik apusan langsung, konsentrasi formalin-eter dan perwarnaan trikrom. Prevalens infeksi protozoa usus Entamoeba histolytica di kalangan kanak-kanak Orang Asli di Pos Lenjang, Pahang adalah tinggi iaitu 22.5%. Dari segi jantina, prevalens infeksi lebih tinggi di kalangan kanak-kanak perempuan (32.5%) berbanding kanak-kanak lelaki (9.7%). Infeksi juga didapati lebih kerap berlaku di kalangan kanak-kanak yang bersekolah (32.4%) berbanding kanak-kanak prasekolah (11.8%). Prevalens infeksi Entamoeba histolytica yang tinggi di kalangan kanak-kanak Orang Asli di Pos Lenjang, Pahang adalah berhubung kait dengan pelbagai faktor termasuk status sosioekonomi yang rendah, budaya, kekurangan kemudahan asas dan tahap pengetahuan mengenai penjagaan kesihatan serta kebersihan diri yang rendah.
    Matched MeSH terms: Formaldehyde
  4. Hartini Yusof, Mohamed Kamel Abd. Ghani
    MyJurnal
    Giardia intestinalis merupakan parasit kosmopolitan dan infeksinya tersebar luas di seluruh dunia terutamanya di negara membangun yang tahap sanitasinya rendah dan kekurangan bekalan air yang bersih. Seramai 71 orang kanak-kanak Orang Asli dari Pos Lenjang, Pahang telah terlibat di dalam kajian ini. Sampel feses dikumpul dan diperiksa bagi mengesan infeksi G. intestinalis dengan menggunakan tiga teknik diagnosis iaitu teknik apusan langsung, konsentrasi formalin-eter dan perwarnaan trikrom. Prevalens infeksi Giardia intestinalis di kalangan kanak-kanak Orang Asli di Pos Lenjang, Pahang adalah tinggi iaitu 43.7%. Dari segi jantina, prevalens infeksi hampir sama di kalangan kanak-kanak perempuan (45.0%) berbanding kanak-kanak lelaki (41.9%). Infeksi juga didapati lebih banyak berlaku di kalangan kanak-kanak bersekolah (48.6%) berbanding kanakkanak pra-sekolah (38.2%).
    Matched MeSH terms: Formaldehyde
  5. Ali Ahmadzadeh, Sarani Zakaria, Maisarah Yusoff
    The liquefaction of oil palm empty fruit bunch (EFB) in phenol was carried out in the presence of sulfuric acid as a catalyst in the reflux condenser system. The effect of initial phenol input and EFB on liquefaction reaction was investigated by measuring the reaction yield, EFB residue and combined phenol. The initial ratio of phenol to EFB has the greatest influence on the residue and reaction yield. The liquefaction products consist of some liquid by products with high amount of OH groups. The amount of this byproduct is 0.5 time of the solid product (phenolic resin).
    Matched MeSH terms: Formaldehyde
  6. Mohamed Kamel Abd. Ghani, Hartini Yusof
    Sains Malaysiana, 2011;40:1123-1127.
    Blastocystis hominis merupakan antara protozoa yang paling biasa ditemui di dalam sampel feses manusia di seluruh dunia. Prevalens infeksi protozoa ini adalah lebih tinggi di kalangan mereka yang tinggal di negara membangun berbanding negara maju. Seramai 71 orang kanak-kanak Orang Asli dari Pos Lenjang, Pahang telah menjadi subjek dalam kajian ini. Bagi kajian yang lebih terperinci, kumpulan kanak-kanak ini telah dibahagikan menurut jantina dan umur. Sampel feses dikumpul dan setiap sampel diperiksa dengan menggunakan 3 teknik diagnostik iaitu teknik apusan langsung, konsentrasi formalin-eter dan perwarnaan trikrom bagi tujuan pengesanan dan pengenalpastian Blastocystis hominis. Prevalens infeksi Blastocystis hominis di kalangan kanak-kanak Orang Asli adalah sangat tinggi iaitu 93%. Kanak-kanak perempuan didapati lebih ramai terinfeksi (97.5%) berbanding kanak-kanak lelaki (87.1%) walaupun secara statistiknya tidak signifikan (p>0.05). Protozoa ini juga telah menginfeksi kesemua kanak-kanak prasekolah (100%) manakala kanak-kanak yang bersekolah turut menunjukkan prevalens infeksi yang tinggi iaitu 86.5%. Daripada segi diagnosis, teknik perwarnaan trikrom didapati paling sensitif dan ia dapat mengenalpasti kesemua (66) sampel feses yang positif dengan Blastocystis hominis. Ini diikuti dengan teknik konsentrasi formalin-eter (43 sampel) dan teknik apusan langsung (18 sampel) (p<0.05). Prevalens infeksi Blastocystis hominis yang tinggi di kalangan kanak-kanak Orang Asli adalah berhubungkait dengan pelbagai faktor termasuk status sosioekonomi yang rendah, budaya, kekurangan kemudahan asas dan tahap pengetahuan mengenai penjagaan kesihatan serta kebersihan diri yang rendah. Selain itu, peningkatan prevalens infeksi dalam kajian ini menunjukkan pentingnya penggunaan teknik diagnostik yang lebih berkesan di dalam pemeriksaan rutin bagi memperolehi hasil diagnosis yang lebih tepat.
    Matched MeSH terms: Formaldehyde
  7. Ali Ahmadzadeh, Sarani Zakaria, Rozaidi Rasid, Sharifah Nabihah
    Sains Malaysiana, 2008;37:233-237.
    Biofiber is used in the polymer based composite as a renewable resource due to its positive environmental benefits, biodegradable properties, low cost and high toughness. Biocomposite was fabricated using oil palm empty fruit bunch (EFB) as filler in phenolated EFB (PEFB) matrix. Phenolated EFB (PEFB) obtained from liquefaction of EFB in phenol was used as a biopolymer to replace novolak phenolic resin which is commonly used in composite materials. Sulfuric acid was used as a catalyst in the liquefaction reaction. The effect of thermal aging and blending ratio of PEFB matrix and EFB fibers on the mechanical properties of composites has been studied. The flexural data before and after thermal aging revealed the optimum amount of EFB filler is 50% . The result showed better compatibility between EFB and PEFB when compared with EFB and commercial novolak resin.
    Matched MeSH terms: Formaldehyde
  8. Mariam Ahmad Zawawi, Mohamed Kamel Abd Ghani, Gopal G, Hidayatulfathi Othman, Hartini Yusof, Norhisham Haron
    Sains Malaysiana, 2012;41:1095-1098.
    entamoeba histolytica dan/atau entamoeba dispar merupakan protozoa usus yang mempunyai prevalens infeksi yang tinggi dalam kalangan masyarakat pedalaman terutamanya masyarakat Orang Asli. Ia tersebar secara meluas di kawasan tropika dan subtropika serta di negara membangun berbanding negara maju. Sebanyak 111 sampel feses kanak-kanak Orang Asli daripada suku kaum Jahai telah diterima dan disaring untuk entamoeba histolytica dan/atau entamoeba dispar menggunakan kaedah apusan langsung yang memberi hasil positif terhadap 43 sampel atau 38.7%. Oleh sebab amaun sampel yang diterima adalah sedikit, hanya 66 sampel feses sahaja yang dapat diperiksa menggunakan tiga jenis teknik diagnostik berbeza iaitu apusan langsung, kepekatan formalin-eter dan pewarnaan trikrom. Hasil kajian mendapati, prevalens yang tinggi bagi infeksi entamoeba histolytica dan/atau entamoeba dispar iaitu 50% menggunakan
    ketiga-tiga teknik diagnosis. Prevalens infeksi yang tinggi juga turut ditunjukkan pada kanak-kanak perempuan iaitu 62.5% berbanding kanak-kanak lelaki 30.8% (p<0.05). Selain itu, daripada segi umur, kanak-kanak yang berumur 7-9 tahun adalah lebih terdedah kepada infeksi entamoeba histolytica dan/atau entamoeba dispar dengan prevalens 60.7% (p>0.05). Teknik pewarnaan trikrom menunjukkan pengesanan 100% ke atas infeksi entamoeba histolytica dan/atau entamoeba dispar, diikuti teknik kepekatan formalin-eter 78.8% dan apusan langsung 72.7%. Prevalens infeksi entamoeba histolytica dan/atau entamoeba dispar yang tinggi dalam kalangan kanak-kanak Orang Asli di Pos Sungai Rual ini berhubungkait dengan pelbagai faktor iaitu status sosioekonomi yang rendah, kekurangan pengetahuan tentang penjagaan kesihatan serta kebersihan diri yang rendah. Peningkatan prevalens infeksi dalam kajian ini juga menunjukkan pentingnya penggunaan teknik diagnostik yang lebih berkesan dalam pemeriksaan rutin bagi mendapatkan hasil diagnosis yang lebih tepat.
    Matched MeSH terms: Formaldehyde
  9. Lim YC, Phang KS, Cheong SK
    Malays J Pathol, 1992 Dec;14(2):85-9.
    PMID: 1304629
    With the advent of new monoclonal antibodies that are applicable to formalin-fixed, paraffin embedded sections, immunophenotyping is becoming increasingly important in the diagnosis and classification of lymphomas. However, multiple factors such as fixation, trypsinization and even type of antibodies used have certain effects on the final outcome of the staining procedure. In this paper we report our experience and the problems encountered in our laboratory when we first tried to establish a workable immunostaining protocol for formalin-fixed, paraffin embedded tissue sections using the immunoalkaline phosphatase technique.
    Matched MeSH terms: Formaldehyde*
  10. Abdullah D, Kanagasingam S, Luke D
    Sains Malaysiana, 2013;42:81-84.
    The aim of the study was to determine the frequency, size and location of apical and lateral foramina on anterior teeth. A total of 100 anterior teeth consisting of maxillary and mandibular incisors and canines were fixed in 10% formalin. Periodontal tissue remnants were mechanically removed and teeth were stained in 2% aqueous silver nitrate. The teeth were dried and examined using a Leica MZ 7.5 zoom stereomicroscope. The size of apical and lateral foramina and their distance from the anatomical apex of the tooth were measured directly using a calibrated eyepiece scale. Accessory foramina more than 1.8 mm from the apex were regarded as lateral foramina. Eighteen percent of teeth possessed more than one apical foramen. Seven teeth (three maxillary centrals, three maxillary canines, one mandibular lateral) had 11 lateral foramina each. The mean diameter of the lateral foramina was 0.14 mm (SD = 0.08) and their mean distance from the apex was 4.49 mm (SD = 2.63, range 1.9-10.5 mm). Multiple foramina were most common on maxillary canines and least common on maxillary laterals. The mean diameter of apical foramina for all teeth possessing a single foramen was 0.35 mm (SD = 0.10) and the mean apical foramen diameter for all teeth with multiple apical foramina was 0.22 mm (SD = 0.08). Most anterior teeth possess one apical foramen and no lateral foramina; about 20% possessed more than one apical foramen and about 10% possessed one or more lateral foramina. These findings should be considered when root-treating anterior teeth.
    Matched MeSH terms: Formaldehyde
  11. Sanusi R, Ab Shukor N, Sulaiman M
    Sains Malaysiana, 2013;42:1511-1516.
    This study was carried out to evaluate the anti-inflammatory effects of three concentrations of Labisia pumila (Blume) F. Vill-Naves aqueous leaf extract in rats. The effects of these extracts as anti-inflammatory agents were determined using two experiments namely formalin-induced paw licking and carrageenan-induced paw oedema test. The exposure of inflammation to various treatments resulted in significant differences between treatments in formalin-induced paw licking in rats experiment whereas in phase 2, 50 mg kg-1 of L. pumila extract showed the most significant inhibition of 82.12%, followed by 10 mg kg-1 with 76.00% and 25 mg kg-1 with 57.80%. Similarly, different treatments showed significant effects at p<0.05 in the carrageenan inducing paw oedema experiment. All treatments were able to suppress the oedema formation induced by carrageenan as compared with the control. It is evident that the anti-inflammtory effect of every concentration of L. pumila extract started as early as the first hour of carrageenan injection and showed the maximum inhibition during the fifth hour. Again, 50 mg kg-1 of L. pumila extract was found to be the best treatment that could reduce inflammation with highest inhibition of 64.59% followed by 25 mg kg-1 with 56.99% and 10 mg kg-1 with 5.55%. The result of this study has shown that these extracts of L. pumila can be effective for anti-inflammation purposes which supports and justifies traditional uses of this plant.
    Matched MeSH terms: Formaldehyde
  12. Shi Yeen TN, Pathmanathan R, Shiran MS, Ahmad Zaid FA, Cheah YK
    J. Biomed. Sci., 2013 Apr 16;20:22.
    PMID: 23590575 DOI: 10.1186/1423-0127-20-22
    BACKGROUND: Somatic mutations of the epidermal growth factor receptor (EGFR) are reportedly associated with various responses in non-small cell lung cancer (NSCLC) patients receiving the anti-EGFR agents. Detection of the mutation therefore plays an important role in therapeutic decision making. The aim of this study was to detect EGFR mutations in formalin fixed paraffin embedded (FFPE) samples using both Scorpion ARMS and high resolution melt (HRM) assay, and to compare the sensitivity of these methods.

    RESULTS: All of the mutations were found in adenocarcinoma, except one that was in squamous cell carcinoma. The mutation rate was 45.7% (221/484). Complex mutations were also observed, wherein 8 tumours carried 2 mutations and 1 tumour carried 3 mutations.

    CONCLUSIONS: Both methods detected EGFR mutations in FFPE samples. HRM assays gave more EGFR positive results compared to Scorpion ARMS.

    Matched MeSH terms: Formaldehyde/metabolism; Formaldehyde/chemistry*
  13. Nengsih S, Umar AA, Salleh MM, Oyama M
    Sensors (Basel), 2012;12(8):10309-25.
    PMID: 23112601 DOI: 10.3390/s120810309
    The effect of morphology on the plasmonic sensing of the presence of formaldehyde in water by gold nanostructures has been investigated. The gold nanostructures with two different morphologies, namely spherical and rod, were prepared using a seed-mediated method. In typical results, it was found that the plasmonic properties of gold nanostructures were very sensitive to the presence of formaldehyde in their surrounding medium by showing the change in both the plasmonic peaks position and the intensity. Spherical nanoparticles (GNS), for example, indicated an increase in the sensitivity when the size was increased from 25 to 35 nm and dramatically decreased when the size was further increased. An m value, the ratio between plasmonic peak shift and refractive index change, as high as 36.5 nm/RIU (refractive index unit) was obtained so far. An expanded sensing mode to FD was obtained when gold nanostructures with nanorods morphology (GNR) were used because of the presence of two plasmonic modes for response probing. However, in the present study, effective plasmonic peak shift was not observed due to the intense plasmonic coupling of closely packed nanorod structures on the surface. Nevertheless, the present results at least provide a potential strategy for response enhancement via shape-effects. High performance plasmonic sensors could be obtained if controlled arrays of nanorods can be prepared on the surface.
    Matched MeSH terms: Formaldehyde/analysis*; Formaldehyde/chemistry
  14. Zulkifli I, Fauziah O, Omar AR, Shaipullizan S, Siti Selina AH
    Vet. Res. Commun., 1999 Mar;23(2):91-9.
    PMID: 10359153
    Two experiments were conducted to evaluate the effect of formaldehyde vaporization of a hatcher on the tracheal epithelium of chick embryos, and on the production performance and behaviour of commercial broiler chicks. In experiment 1, chick embryos were exposed to 23.5 ppm of formaldehyde vapour during the last 3 days of incubation. Tracheal samples were taken at 0, 6, 30 and 54 h after exposure to formaldehyde and examined by scanning electron microscopy for pathological changes. Observable lesions included excessive accumulation of mucus, matted cilia, loss of cilia and sloughing of the epithelium. The lesions were more severe in chicks exposed for 54 h as compared to those exposed for 6 or 30 h. In experiment 2, 60 chicks that had been exposed to formaldehyde vapour as above and 60 control chicks were used to investigate the effect of formaldehyde fumigation on production performance and behaviour. Formaldehyde vaporization resulted in higher weekly (days 0-6 and 21-27) and total (days 0-41) feed intake and poorer weekly (days 0-6, 7-13, 21-27 and 28-34) and overall (days 0-41) feed conversion ratios. Body weight, mortality and behaviour (eating, drinking, sitting and standing activities) were not affected by formaldehyde fumigation.
    Matched MeSH terms: Formaldehyde/administration & dosage; Formaldehyde/pharmacology*
  15. Ramesh G, Khamizar W
    Med. J. Malaysia, 2005 Jun;60(2):201-3.
    PMID: 16114161
    The use of high voltage radiotheraphy in pelvi malignancies has led to a common complication which is radiation . proctitis. Treatment with rectal formalin is simple and effective and can be done as a day care procedure. The use of formalin dab was assessed as a day care procedure in our surgical unit in which ten patients were prospectively evaluated. Outcome of these patients was encouraging.
    Matched MeSH terms: Formaldehyde/administration & dosage; Formaldehyde/therapeutic use*
  16. Long I, Suppian R, Ismail Z
    Korean J Pain, 2013 Jul;26(3):255-64.
    PMID: 23861999 DOI: 10.3344/kjp.2013.26.3.255
    We investigated the effects of pre-emptive administration of ketamine and norBNI on pain behavior and the expression of DREAM, c-Fos, and prodynorphin proteins on the ipsilateral side of the rat spinal cord at 2 and 4 hours after formalin injection.
    Matched MeSH terms: Formaldehyde
  17. Ambu, Stephen, Yin, Evelyn Synn Yeoh, Joon, Wah Mak, Chakravarthi, Srikumar
    MyJurnal
    The aim of the study was to assess the prevalence of Sarcocystis infection in wild and peri-urban rodents in some states in Peninsular Malaysia. The thigh muscle from these rodents were formalin preserved, sectioned and stained with hematoxylin and eosin and examined under light microscopy. Of the 146 muscle tissue examined only 73 were positive for Sarcocystis infection.
    Morphological identification showed the presence of some new morphological types to be present. Different species of Sarcocystis were seen in the sections but more extensive studies are needed to identify them to species level.
    Matched MeSH terms: Formaldehyde
  18. Choon, Y.F., Ramanathan, A., Ali, H., Ghani, W.M.N., Cheong, S.C., Zain, R.B.
    Ann Dent, 2011;18(1):8-17.
    MyJurnal
    Background: MDM2 and p53 are involved in a negative feedback loop where p53 regulates MDM2 at the transcriptional level. MDM2, in turn, downregulates p53. This co-ordinated interaction between these proteins is set to play an important role in the regulation of cell cycle progression following DNA damage to cells. The over-expression of both p53 and MDM2 has been reported in various cancers. However there are only few studies discussing the co-expression of MDM2 with p53 in oral squamous cell carcinoma Aim: The purpose of this study was to determine the correlation of co-expression of p53, MDM2, and Ki-67 proteins with clinico-pathological factors in oral squamous cell carcinoma (OSCC) and to conduct a systematic review of the co-expression of p53/MDM2.

    Method: This is a retrospective descriptive study and a systematic review. Formalin-fixed paraffinembedded tissues from 45 OSCC cases were stained by immunohistochemistry (IHC) for p53, MDM2, and Ki-67 proteins.

    Results: Immuno-reactivity for p53, MDM2, and Ki-67 was seen in 75.6%, 97.8%, and 62.2% cases of OSCC respectively. The co-expression of p53 and MDM2 (p53/MDM2) was detected in 97.1%, however there was no significant correlation between p53 and MDM2 expression. Notably, p53/MDM2 coexpression was significantly associated with tumour differentiation (p-value = 0.045). The Ki-67LI was not significantly associated with neither MDM2 nor p53/MDM2 co-expression (p-value = 0.268, 0.916 respectively).

    Conclusion: The expression of MDM2 was not signif icantly associated with p53 expression suggesting that MDM2 expression is mediated by p53-independent pathways or mutated p53 could not induce the expression of MDM2 in this set of OSCCs. The only clinico-pathological parameter that correlates significantly with co-expression of p53/MDM2 is tumour differentiation where it is suggestive that the co-expression of these 2 proteins is indicative of aggressive tumour behavior.
    Matched MeSH terms: Formaldehyde
  19. Lian, C.B., Ngeow, W.C.
    Ann Dent, 2000;7(1):-.
    MyJurnal
    Formalin is a clear solution of 37% formaldehyde in water. It is used in dentistry as a disinfectant, antiseptic and mainly as tissue fixative for preserving biologic specimens for histopathologic examination. The human knowledge on systemic formaldehyde intoxication is inadequate as only few cases of formalin ingestion have been reported. This paper presents a brief communication of the adverse effect of formalin to the human tissue.
    Matched MeSH terms: Formaldehyde
  20. Nor Mohd Razif Noraini, Leman, A.M., Ahmad Sayuti Zainal Abidin
    MyJurnal
    A preliminary study has been conducted in a new constructed 8 stories building (2 level of Hostels facility, 3 level of Training Room, 2 Level of Offices and 1 level of Exhibition Halls and Rooms) in Bandar Baru Bangi, Selangor. The Hostels facility is a floor tile and furnished with build in locker and use split air conditioning system while the Training Room and Exhibition Rooms used floor tile. The Offices and Exhibition Hall are carpeted furnished. All these spaces were using centralized air conditioning systems. A pre-commissioning assessment on 5 chemical parameters of indoor air pollutants such Total Volatile Organic Compounds (TVOC), Formaldehyde, Respirable Particulates (PM!
    Matched MeSH terms: Formaldehyde
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