Displaying publications 1 - 20 of 86 in total

  1. Lee SH, H'ng PS, Peng TL, Lum WC
    Pak. J. Biol. Sci., 2013 Nov 1;16(21):1415-8.
    PMID: 24511759
    The objective of this study is to investigate the feasibility of formaldehyde catcher as termites repellent. Single-layered UF-bonded particleboard was post-treated with formaldehyde catcher and heat respectively. Besides that, some boards were also produced with the formaldehyde catcher was added into the resin during the blending process, called add-in method. Particleboard post-treated with formaldehyde catcher reported the most severe attack. Heat-treated particleboard showed slightly better durability than the control blocks while the add-in catcher showed the best durability among three methods. A valid test was obtained as the termites survived the first week of the test. However, all the termites were found dead at the end of the test.
    Matched MeSH terms: Formaldehyde*
  2. Hartini Yusof, Mohamed Kamel Abd Ghani
    Infeksi Entamoeba histolytica adalah tersebar di seluruh dunia dengan prevalens infeksi yang lebih tinggi di kalangan masyarakat terpinggir termasuk Orang Asli yang tinggal di kawasan tropika dan subtropika. Seramai 71 orang kanak-kanak Orang Asli dari Pos Lenjang, Pahang telah terlibat di dalam kajian ini. Bagi kajian yang lebih terperinci, kumpulan kanak-kanak ini telah dibahagikan menurut jantina dan umur. Sampel feses dikumpul dan setiap sampel diperiksa bagi pengenalpastian Entamoeba histolytica dengan menggunakan 3 jenis teknik diagnostik yang berbeza iaitu teknik apusan langsung, konsentrasi formalin-eter dan perwarnaan trikrom. Prevalens infeksi protozoa usus Entamoeba histolytica di kalangan kanak-kanak Orang Asli di Pos Lenjang, Pahang adalah tinggi iaitu 22.5%. Dari segi jantina, prevalens infeksi lebih tinggi di kalangan kanak-kanak perempuan (32.5%) berbanding kanak-kanak lelaki (9.7%). Infeksi juga didapati lebih kerap berlaku di kalangan kanak-kanak yang bersekolah (32.4%) berbanding kanak-kanak prasekolah (11.8%). Prevalens infeksi Entamoeba histolytica yang tinggi di kalangan kanak-kanak Orang Asli di Pos Lenjang, Pahang adalah berhubung kait dengan pelbagai faktor termasuk status sosioekonomi yang rendah, budaya, kekurangan kemudahan asas dan tahap pengetahuan mengenai penjagaan kesihatan serta kebersihan diri yang rendah.
    Matched MeSH terms: Formaldehyde
  3. Hartini Yusof, Mohamed Kamel Abd. Ghani
    Giardia intestinalis merupakan parasit kosmopolitan dan infeksinya tersebar luas di seluruh dunia terutamanya di negara membangun yang tahap sanitasinya rendah dan kekurangan bekalan air yang bersih. Seramai 71 orang kanak-kanak Orang Asli dari Pos Lenjang, Pahang telah terlibat di dalam kajian ini. Sampel feses dikumpul dan diperiksa bagi mengesan infeksi G. intestinalis dengan menggunakan tiga teknik diagnosis iaitu teknik apusan langsung, konsentrasi formalin-eter dan perwarnaan trikrom. Prevalens infeksi Giardia intestinalis di kalangan kanak-kanak Orang Asli di Pos Lenjang, Pahang adalah tinggi iaitu 43.7%. Dari segi jantina, prevalens infeksi hampir sama di kalangan kanak-kanak perempuan (45.0%) berbanding kanak-kanak lelaki (41.9%). Infeksi juga didapati lebih banyak berlaku di kalangan kanak-kanak bersekolah (48.6%) berbanding kanakkanak pra-sekolah (38.2%).
    Matched MeSH terms: Formaldehyde
  4. Edwin Shiaw CS, Shiran MS, Cheah YK, Tan GC, Sabariah AR
    Med. J. Malaysia, 2010 Jun;65(2):133-7.
    PMID: 23756798 MyJurnal
    This study was done to evaluate various DNA and RNA extractions from archival FFPE tissues. A total of 30 FFPE blocks from the years of 2004 to 2006 were assessed with each modified and adapted method. Extraction protocols evaluated include the modified enzymatic extraction method (Method A), Chelex-100 extraction method (Method B), heat-induced retrieval in alkaline solution extraction method (Methods C and D) and one commercial FFPE DNA Extraction kit (Qiagen, Crawley, UK). For RNA extraction, 2 extraction protocols were evaluated including the enzymatic extraction method (Method 1), and Chelex-100 RNA extraction method (Method 2). Results show that the modified enzymatic extraction method (Method A) is an efficient DNA extraction protocol, while for RNA extraction, the enzymatic method (Method 1) and the Chelex-100 RNA extraction method (Method 2) are equally efficient RNA extraction protocols.
    Matched MeSH terms: Formaldehyde
  5. Lim YC, Phang KS, Cheong SK
    Malays J Pathol, 1992 Dec;14(2):85-9.
    PMID: 1304629
    With the advent of new monoclonal antibodies that are applicable to formalin-fixed, paraffin embedded sections, immunophenotyping is becoming increasingly important in the diagnosis and classification of lymphomas. However, multiple factors such as fixation, trypsinization and even type of antibodies used have certain effects on the final outcome of the staining procedure. In this paper we report our experience and the problems encountered in our laboratory when we first tried to establish a workable immunostaining protocol for formalin-fixed, paraffin embedded tissue sections using the immunoalkaline phosphatase technique.
    Matched MeSH terms: Formaldehyde*
  6. Shi Yeen TN, Pathmanathan R, Shiran MS, Ahmad Zaid FA, Cheah YK
    J. Biomed. Sci., 2013 Apr 16;20:22.
    PMID: 23590575 DOI: 10.1186/1423-0127-20-22
    BACKGROUND: Somatic mutations of the epidermal growth factor receptor (EGFR) are reportedly associated with various responses in non-small cell lung cancer (NSCLC) patients receiving the anti-EGFR agents. Detection of the mutation therefore plays an important role in therapeutic decision making. The aim of this study was to detect EGFR mutations in formalin fixed paraffin embedded (FFPE) samples using both Scorpion ARMS and high resolution melt (HRM) assay, and to compare the sensitivity of these methods.

    RESULTS: All of the mutations were found in adenocarcinoma, except one that was in squamous cell carcinoma. The mutation rate was 45.7% (221/484). Complex mutations were also observed, wherein 8 tumours carried 2 mutations and 1 tumour carried 3 mutations.

    CONCLUSIONS: Both methods detected EGFR mutations in FFPE samples. HRM assays gave more EGFR positive results compared to Scorpion ARMS.

    Matched MeSH terms: Formaldehyde/metabolism; Formaldehyde/chemistry*
  7. Nengsih S, Umar AA, Salleh MM, Oyama M
    Sensors (Basel), 2012;12(8):10309-25.
    PMID: 23112601 DOI: 10.3390/s120810309
    The effect of morphology on the plasmonic sensing of the presence of formaldehyde in water by gold nanostructures has been investigated. The gold nanostructures with two different morphologies, namely spherical and rod, were prepared using a seed-mediated method. In typical results, it was found that the plasmonic properties of gold nanostructures were very sensitive to the presence of formaldehyde in their surrounding medium by showing the change in both the plasmonic peaks position and the intensity. Spherical nanoparticles (GNS), for example, indicated an increase in the sensitivity when the size was increased from 25 to 35 nm and dramatically decreased when the size was further increased. An m value, the ratio between plasmonic peak shift and refractive index change, as high as 36.5 nm/RIU (refractive index unit) was obtained so far. An expanded sensing mode to FD was obtained when gold nanostructures with nanorods morphology (GNR) were used because of the presence of two plasmonic modes for response probing. However, in the present study, effective plasmonic peak shift was not observed due to the intense plasmonic coupling of closely packed nanorod structures on the surface. Nevertheless, the present results at least provide a potential strategy for response enhancement via shape-effects. High performance plasmonic sensors could be obtained if controlled arrays of nanorods can be prepared on the surface.
    Matched MeSH terms: Formaldehyde/analysis*; Formaldehyde/chemistry
  8. Zulkifli I, Fauziah O, Omar AR, Shaipullizan S, Siti Selina AH
    Vet. Res. Commun., 1999 Mar;23(2):91-9.
    PMID: 10359153
    Two experiments were conducted to evaluate the effect of formaldehyde vaporization of a hatcher on the tracheal epithelium of chick embryos, and on the production performance and behaviour of commercial broiler chicks. In experiment 1, chick embryos were exposed to 23.5 ppm of formaldehyde vapour during the last 3 days of incubation. Tracheal samples were taken at 0, 6, 30 and 54 h after exposure to formaldehyde and examined by scanning electron microscopy for pathological changes. Observable lesions included excessive accumulation of mucus, matted cilia, loss of cilia and sloughing of the epithelium. The lesions were more severe in chicks exposed for 54 h as compared to those exposed for 6 or 30 h. In experiment 2, 60 chicks that had been exposed to formaldehyde vapour as above and 60 control chicks were used to investigate the effect of formaldehyde fumigation on production performance and behaviour. Formaldehyde vaporization resulted in higher weekly (days 0-6 and 21-27) and total (days 0-41) feed intake and poorer weekly (days 0-6, 7-13, 21-27 and 28-34) and overall (days 0-41) feed conversion ratios. Body weight, mortality and behaviour (eating, drinking, sitting and standing activities) were not affected by formaldehyde fumigation.
    Matched MeSH terms: Formaldehyde/administration & dosage; Formaldehyde/pharmacology*
  9. Ramesh G, Khamizar W
    Med. J. Malaysia, 2005 Jun;60(2):201-3.
    PMID: 16114161
    The use of high voltage radiotheraphy in pelvi malignancies has led to a common complication which is radiation . proctitis. Treatment with rectal formalin is simple and effective and can be done as a day care procedure. The use of formalin dab was assessed as a day care procedure in our surgical unit in which ten patients were prospectively evaluated. Outcome of these patients was encouraging.
    Matched MeSH terms: Formaldehyde/administration & dosage; Formaldehyde/therapeutic use*
  10. Lian, C.B., Ngeow, W.C.
    Ann Dent, 2000;7(1):-.
    Formalin is a clear solution of 37% formaldehyde in water. It is used in dentistry as a disinfectant, antiseptic and mainly as tissue fixative for preserving biologic specimens for histopathologic examination. The human knowledge on systemic formaldehyde intoxication is inadequate as only few cases of formalin ingestion have been reported. This paper presents a brief communication of the adverse effect of formalin to the human tissue.
    Matched MeSH terms: Formaldehyde
  11. Choon, Y.F., Ramanathan, A., Ali, H., Ghani, W.M.N., Cheong, S.C., Zain, R.B.
    Ann Dent, 2011;18(1):8-17.
    Background: MDM2 and p53 are involved in a negative feedback loop where p53 regulates MDM2 at the transcriptional level. MDM2, in turn, downregulates p53. This co-ordinated interaction between these proteins is set to play an important role in the regulation of cell cycle progression following DNA damage to cells. The over-expression of both p53 and MDM2 has been reported in various cancers. However there are only few studies discussing the co-expression of MDM2 with p53 in oral squamous cell carcinoma Aim: The purpose of this study was to determine the correlation of co-expression of p53, MDM2, and Ki-67 proteins with clinico-pathological factors in oral squamous cell carcinoma (OSCC) and to conduct a systematic review of the co-expression of p53/MDM2.

    Method: This is a retrospective descriptive study and a systematic review. Formalin-fixed paraffinembedded tissues from 45 OSCC cases were stained by immunohistochemistry (IHC) for p53, MDM2, and Ki-67 proteins.

    Results: Immuno-reactivity for p53, MDM2, and Ki-67 was seen in 75.6%, 97.8%, and 62.2% cases of OSCC respectively. The co-expression of p53 and MDM2 (p53/MDM2) was detected in 97.1%, however there was no significant correlation between p53 and MDM2 expression. Notably, p53/MDM2 coexpression was significantly associated with tumour differentiation (p-value = 0.045). The Ki-67LI was not significantly associated with neither MDM2 nor p53/MDM2 co-expression (p-value = 0.268, 0.916 respectively).

    Conclusion: The expression of MDM2 was not signif icantly associated with p53 expression suggesting that MDM2 expression is mediated by p53-independent pathways or mutated p53 could not induce the expression of MDM2 in this set of OSCCs. The only clinico-pathological parameter that correlates significantly with co-expression of p53/MDM2 is tumour differentiation where it is suggestive that the co-expression of these 2 proteins is indicative of aggressive tumour behavior.
    Matched MeSH terms: Formaldehyde
  12. Nor Mohd Razif Noraini, Leman, A.M., Ahmad Sayuti Zainal Abidin
    A preliminary study has been conducted in a new constructed 8 stories building (2 level of Hostels facility, 3 level of Training Room, 2 Level of Offices and 1 level of Exhibition Halls and Rooms) in Bandar Baru Bangi, Selangor. The Hostels facility is a floor tile and furnished with build in locker and use split air conditioning system while the Training Room and Exhibition Rooms used floor tile. The Offices and Exhibition Hall are carpeted furnished. All these spaces were using centralized air conditioning systems. A pre-commissioning assessment on 5 chemical parameters of indoor air pollutants such Total Volatile Organic Compounds (TVOC), Formaldehyde, Respirable Particulates (PM!
    Matched MeSH terms: Formaldehyde
  13. Ambu, Stephen, Yin, Evelyn Synn Yeoh, Joon, Wah Mak, Chakravarthi, Srikumar
    The aim of the study was to assess the prevalence of Sarcocystis infection in wild and peri-urban rodents in some states in Peninsular Malaysia. The thigh muscle from these rodents were formalin preserved, sectioned and stained with hematoxylin and eosin and examined under light microscopy. Of the 146 muscle tissue examined only 73 were positive for Sarcocystis infection.
    Morphological identification showed the presence of some new morphological types to be present. Different species of Sarcocystis were seen in the sections but more extensive studies are needed to identify them to species level.
    Matched MeSH terms: Formaldehyde
  14. Omar, Naja Nadiera, Iskandar Shahrim Mustafa, Nurhayati Abdullah, Rokiah Hashim
    Phenol Formaldehyde (PF) resin has been extensively used in the manufacturing industry as a binding agent, especially in the production of wood-based panels because of its ability to provide good moisture resistance, exterior strength and durability as well as excellent temperature stability. However, due to the use of limited petroleum-based phenol in its formulation, there is a strong interest in exploring renewable biomass material to partially substitute the petroleum-based phenol. In this study, the slow pyrolysis of biomass decomposition process was used to convert two types of biomass, namely, oil palm frond and Rhizophora hardwood, into bio-oil. The phenol-rich fraction of the bio-oil was separated and added into the formulation of PF resin to produce an environmentally-friendly type of PF resin, known as bio-oilphenol-formaldehyde (BPF) resin. This BPF resin was observed to have comparable viscosity, better alkalinity, improved non-volatile content and faster curing temperature than conventional PF resin. Moreover, the particleboard bonded with this BPF resin was observed to have just as excellent bonding strength as the one bonded using conventional PF resin. However, the BPF resin exhibited an increased level of free formaldehyde and less thermal stability than the conventional PF resin, probably due to the addition of the less reactive bio-oil.
    Matched MeSH terms: Formaldehyde
  15. Affandi KA, Tizen NMS, Mustangin M, Zin RRMRM
    J Pathol Transl Med, 2018 Sep;52(5):283-289.
    PMID: 30235512 DOI: 10.4132/jptm.2018.08.14
    BACKGROUND: Lung cancer is the third most common cancer worldwide. With major advances in the molecular testing of lung cancers and the introduction of targeted therapies, the distinction between adenocarcinoma and squamous cell carcinoma as well as pathologic subtyping has become important. Recent studies showed that p40 is highly specific for squamous and basal cells and is superior to p63 for diagnosing lung squamous cell carcinoma. The aim of this study was to evaluate the use of p40 immunohistochemical stain in the diagnosis of non-small cell lung carcinoma and its potential to replace current p63 antibody as the best immunohistochemical squamous marker.

    METHODS: Seventy formalin-fixed paraffin-embedded cases previously diagnosed as primary lung squamous cell carcinoma (n = 35) and lung adenocarcinoma (n = 35) from January 2008 to December 2016 were retrieved. The results of tumour cell immunoreactivity for p40 and p63 antibodies in lung squamous cell carcinoma and lung adenocarcinoma were compared.

    RESULTS: p40 was expressed in 27 cases of lung squamous cell carcinoma (77.1%). All cases of lung adenocarcinoma (35/35, 100%) were negative for p40. p63 expression was positive in 30 cases of lung squamous cell carcinoma (85.7%) and 13 cases of lung adenocarcinoma (37.1%). Reactivity for both p40 and p63 in lung squamous cell carcinoma was strong and diffuse, whereas variable reactivity was observed in lung adenocarcinoma.

    CONCLUSIONS: p40 is an excellent marker for distinguishing lung squamous cell carcinoma from adenocarcinoma, and p40 expression is equivalent to p63 expression in lung squamous cell carcinoma.

    Matched MeSH terms: Formaldehyde
  16. Martinez RC, Sathasivam HP, Cosway B, Paleri V, Fellows S, Adams J, et al.
    Br J Oral Maxillofac Surg, 2018 05;56(4):332-337.
    PMID: 29628167 DOI: 10.1016/j.bjoms.2018.03.011
    Our aim was to examine the clinicopathological features of squamous cell carcinoma (SCC) of the oral cavity and oropharynx in a group of young patients who were dignosed during a 15-year period (2000-2014). Patients' clinical details, risk factors, and survival were obtained from medical records. Formalin-fixed, paraffin-embedded, tissue was tested for high-risk human papillomavirus (HPV). The results were compared with those of a matching group of older patients. We identified 91 patients who were younger than 45 years old, and the 50 youngest patients were studied in detail. The male:female ratio was 2:1, with more tumours located in the oral cavity than in the oropharynx (35 compared with 15). HPV-related SCC was restricted to the oropharynx. When matched for site, stage and HPV status, five-year overall survival was similar in young and matched older patients (log-rank test, p=0.515). Our findings suggest that young patients with oral SCC have a disease profile similar to that of older patients with the condition. It is plausible that prognostic information generally available for oral cancers is applicable to young patients with the disease.
    Matched MeSH terms: Formaldehyde
  17. Al Shehadat S, Gorduysus MO, Abdul Hamid SS, Abdullah NA, Samsudin AR, Ahmad A
    Eur J Dent, 2018 10 30;12(4):574-578.
    PMID: 30369805 DOI: 10.4103/ejd.ejd_401_17
    Objective: The objective of this study was to evaluate and compare the two scanning electron microscope (SEM) preparation protocols and determine the better SEM preparation technique to study stem cells on human amniotic membrane (hAM) scaffold.

    Materials and Methods: Formaldehyde-based protocol and glutaraldehyde-based protocol were compared to evaluate the quality of SEM images for stem cells cultured on hAM scaffold.

    Results: The results suggested that formaldehyde-based protocol is better than glutaraldehyde-based protocol in terms of showing clearer topography of the membrane as well as the boarders of the cells. To provide intact surface of the SEM sample and avoid possible ruptures of the hAM or the thin cell layer, it is recommended to perform the dehydration step using graded alcohol concentrations of 20%, 30%, 40%, 50%, 60%, 70%, 80%, and 90%, one time for each and twice in 100% for 10 min each. Gold sputter-coating step is not recommended as it does not improve the image quality.

    Conclusions: To obtain clear SEM images, it is recommended to run a preliminary study to determine the better chemicals and conditions of sample preparation even when following preexisting protocols.

    Matched MeSH terms: Formaldehyde
  18. Alias MIM, Hambali K, Amir A, Fauzi N, Hassin H, Yin SA
    Trop Life Sci Res, 2019 Jan;30(1):161-167.
    PMID: 30847039 DOI: 10.21315/tlsr2019.30.1.10
    A survey was conducted to determine the diversity of fish in Pergau Lake, Jeli, Kelantan, Malaysia from 2 September to 18 October of 2016. Fish samples were collected at seven random sampling stations around the lake by using setting trap method. The collected samples were preserved in 10% formalin solution and delivered to the laboratory for further identification process. Fourteen species, namely as Hemibagrus nemurus, Hampala macrolepidota, Clarias batrachus, Channa striata, Cyprinus carpio, Poropuntius smedleyi, Pangasius sp., Oreochromis niloticus, Oreochromis mossambicus, Leptobarbus hoevenii, Neolissochilus hexagonolepis, Tor tambroides, Osteochilus hasselti and Neolissochilus soroides, comprising of six families were collected during this study. From this study, family of Cyprinidae was the dominant species in Pergau Lake with exactly 50% of catch percentage, followed by family of Bagridae (29%), Cichlidae (10%), Clariidae (5%), Channidae (3%) and Pangasiidae (3%). This study shows that there is still a diversity of fish species in Pergau Lake, showing that the water and its environmental condition is very good.
    Matched MeSH terms: Formaldehyde
  19. Abd Ghani Aizat, Paiman, Bawon, Lee, Seng Hua, Ashaari Zaidon
    In this study, the effects of addition of ammonium and aluminium-based hardeners into
    urea formaldehyde resin (UF) on the physico-mechanical properties and formaldehyde
    emission of the rubberwood particleboard were investigated. Four types of hardeners,
    namely ammonium chloride (AC), ammonium sulphate (AS), aluminium chloride (AlC)
    and aluminium sulphate (AlS), were added into UF resin. The acidity, gelation time,
    viscosity and free formaldehyde content of the UF/hardener mixtures were determined.
    Particleboard made with the UF/hardener mixtures were tested for physico-mechanical
    properties and formaldehyde emission. The pH values of the resin after addition of
    aluminium-based hardeners were higher and resulted in higher viscosity and shorter
    gelation time. Consequently, despite lower formaldehyde emission was recorded, the
    physico-mechanical properties of the resulted particleboard were inferior compared to
    that of ammonium-based hardeners. The best quality particleboard in terms of mechanical,
    physical and formaldehyde emission were obtained from the particleboard made with AS,
    followed by AC.
    Matched MeSH terms: Formaldehyde
  20. Omotoso GO, Kadir RE, Sulaimon FA, Jaji-Sulaimon R, Gbadamosi IT
    Malays J Med Sci, 2018 Sep;25(5):35-47.
    PMID: 30914861 DOI: 10.21315/mjms2018.25.5.4
    Background and aim: This study aimed to determine the effect of gestational nicotine exposure before neurodevelopment on the morphology and histology of the prefrontal cortex (PFC) in rats.

    Methodology: Adult female Wistar rats were time-mated and grouped into three categories: (a) control-given 0.1 mL of normal saline, (b) low-dose nicotine-given 6.88 mg/ kg/d/0.05 mL, and (c) high-dose nicotine-given 13.76 mg/kg/d/0.1 mL in two divided doses. Treatment was given intraperitoneally from gestational days 2 to 6. On postnatal day 15 (P15), the pups were separated from their mothers, anaesthetised and sacrificed, followed by intracardial perfusion with 4% paraformaldehyde. PFC was excised from the brain and processed for tissue histology, histochemistry, and morphology of brain cells.

    Results: Gestational nicotine exposure during the first week of gestation in rats significantly reduced birth weights in nicotine-treated groups compared with control; it, however, accelerated body weights, altered neuronal morphology, and elevated astrocytic count significantly, while oligodendroglial count was slightly increased in the PFC of juvenile rats examined at P15.

    Conclusion: These alterations revealed that gestational nicotine exposure before the commencement of the cellular processes involved in brain development negatively affects neurodevelopment, and this could result in neurological dysfunctions in later life.

    Matched MeSH terms: Formaldehyde
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