Displaying publications 1 - 20 of 246 in total

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  1. Mohd Shah NA, Wan Abdul Wahab WN, Mohd Nawi SF, Mohd-Zain Z, Latif B, Suhaimi R
    Malays J Pathol, 2015 Dec;37(3):271-4.
    PMID: 26712674 MyJurnal
    Entamoeba histolytica, the causative agent for human amoebiasis, is among the most deadly parasites, accounting for the second highest mortality rate among parasitic diseases. Because this parasite dwells in low oxygen tension, for its cultivation, microaerophilic conditions are required to mimick the human gut environment. Several methods developed for optimal growth environment are commercially available and some are conventionally modified in-house which include the Anaerocult A and oil blocking preparation methods. This study was undertaken to compare the reliability of the Anaerocult A and the oil blocking methods in generating anaerobic environment for cultivation of E. histolytica. The trophozoites of E. histolytica HM1: IMSS strains were axenically cultivated in TYI-S-33 medium in culture incubated anaerobically by using Anaerocult A (Merck) and mineral oil blocking method. The outcomes of both methods were determined by the minimum inhibitory concentration (MIC) of metronidazole against E. histolytica by giving a score to the growth pattern of the trophozoites. The reliability of both methods was assessed based on susceptibility testing of E. histolytica to metronidazole. The MIC obtained by both anaerobic condition methods was 6.25 ug/ ml, thus showing that oil-blocking method is comparable to the Anaerocult A method and therefore, considered as a reliable method for generating an anaerobic environment for the cultivation of E. histolytica.
    Matched MeSH terms: In Vitro Techniques
  2. Ponnampalam JT, Musa J
    Med J Malaya, 1965 Dec;20(2):144-5.
    PMID: 4221975
    Matched MeSH terms: In Vitro Techniques
  3. Subrahmanyam C
    Med J Malaya, 1966 Mar;20(3):234-9.
    PMID: 4223073
    Matched MeSH terms: In Vitro Techniques
  4. Sivanandam S, Yap Loy Fong
    Med J Malaya, 1965 Sep;20(1):63-4.
    PMID: 4221424
    Matched MeSH terms: In Vitro Techniques
  5. Tang PW, Chua PS, Chong SK, Mohamad MS, Choon YW, Deris S, et al.
    Recent Pat Biotechnol, 2015;9(3):176-97.
    PMID: 27185502
    BACKGROUND: Predicting the effects of genetic modification is difficult due to the complexity of metabolic net- works. Various gene knockout strategies have been utilised to deactivate specific genes in order to determine the effects of these genes on the function of microbes. Deactivation of genes can lead to deletion of certain proteins and functions. Through these strategies, the associated function of a deleted gene can be identified from the metabolic networks.

    METHODS: The main aim of this paper is to review the available techniques in gene knockout strategies for microbial cells. The review is done in terms of their methodology, recent applications in microbial cells. In addition, the advantages and disadvantages of the techniques are compared and discuss and the related patents are also listed as well.

    RESULTS: Traditionally, gene knockout is done through wet lab (in vivo) techniques, which were conducted through laboratory experiments. However, these techniques are costly and time consuming. Hence, various dry lab (in silico) techniques, where are conducted using computational approaches, have been developed to surmount these problem.

    CONCLUSION: The development of numerous techniques for gene knockout in microbial cells has brought many advancements in the study of gene functions. Based on the literatures, we found that the gene knockout strategies currently used are sensibly implemented with regard to their benefits.

    Matched MeSH terms: In Vitro Techniques/methods
  6. Kutty MK, Bau K
    Med J Malaya, 1969 Dec;24(2):151-3.
    PMID: 4244142
    Matched MeSH terms: In Vitro Techniques
  7. Coombs GL, Sandosham AA
    Med J Malaya, 1965 Sep;20(1):53.
    PMID: 4158839
    Matched MeSH terms: In Vitro Techniques
  8. Hasbullah NA, Taha RM, Awal A
    Pak J Biol Sci, 2008 Jun 01;11(11):1449-54.
    PMID: 18817245
    Regeneration potentials in Gerbera jamesonii Bolus ex. Hook f. from tissues culture system was studied using leaf, petiole and root explants. In vitro regeneration, callus induction and root formation were optimized by manipulation of growth regulators during organogenesis. Various kinds of plant growth regulators such as 6-Benzylaminopurine (BAP), alpha-Naphthalene acetic acid (NAA), 2, 4-Dichlorophenoxyacetic acid (2,4-D), Indole-3-acetic acid (IAA), Indole-3-Butyric acid (IBA), N6-[2-Isopentenyl]adenine (2iP), Kinetin and Zeatin were used to initiate cultures. These plant growth regulators were added to Murashige and Skoog medium in different combinations and concentrations. Adventitious shoots were obtained from petiole explants cultured on Murashige and Skoog (MS) medium supplemented with 2.0 mg L(-1) BAP and 0.5 mg L(-1) NAA. Effectiveness of shoot regeneration medium, type of growth regulator used and duration of induction period were investigated. Leaf explants cultured on MS medium supplemented with 1.0 mg L(-1) BAP and 2.0 mg L(-1) 2, 4-D showed the best results for callus induction. Root explants were found to be non-regenerative in all experiments conducted. Petiole segment was identified as the best explant for regeneration of this species. Regenerated plants were rooted on Murashige and Skoog basal medium. Plantlets were then transferred to field with 75% survival rate.
    Matched MeSH terms: In Vitro Techniques
  9. Kurazono H, Yamasaki S, Ratchtrachenchai O, Nair GB, Takeda Y
    Microbiol. Immunol., 1996;40(4):303-5.
    PMID: 8709866
    Vibrio cholerae O139 isolated from different countries, as well as from different locations within a country, were examined using macrorestriction DNA analysis to determine the clonality of the O139 strains. NotI digests of genomic DNA of representative strains from Nepal, India, Bangladesh, China, Thailand, and Malaysia revealed very similar but not identical patterns. Examinations of the banding patterns generated by pulsed-field gel electrophoresis of strains isolated within countries revealed complete homogeneity. These results further reiterate the spread of an identical clone of V. cholerae O139 although it appears that genetic polymorphism among the O139 strains is becoming apparent.
    Matched MeSH terms: In Vitro Techniques
  10. Wan Abas WA
    Biomed Mater Eng, 1995;5(2):59-63.
    PMID: 7655319
    The response of human skin to "stress relaxation" tests at low loads in vitro was investigated. A number of behaviours, other than those already well established and documented, were observed. The significant behaviours are pure recovery and relaxation-recovery. Other behaviours observed are temporary stress recovery during the relaxation process, and momentary sudden non-linear drop in stress value followed by a second relaxation. The pure recovery and relaxation-recovery responses are repeatable. The latter represents the transitional response between the well-known behaviour of stress relaxation and the behaviour of stress recovery.
    Matched MeSH terms: In Vitro Techniques
  11. Chapple PJ
    Bull World Health Organ, 1966;34(2):243-8.
    PMID: 5296130
    Studies have recently been published of surveys of antibodies to common respiratory viruses in human sera from several parts of the world. The present article reports the findings of a survey of antibodies to two more viruses (adenovirus type 8 and coxsackievirus type A21) in human sera mainly collected from six widely separated geographical regions (Alaska, England, Marshall Islands, Sarawak, South-West Africa and Tunisia).A world-wide geographical distribution of infection with these two viruses was found. However, antibodies to individual viruses were not found with the same frequency in all countries; and, in marked contrast to the findings in the earlier surveys of antibodies to the common respiratory viruses, the frequency of antibodies was not the same for each virus in sera from the same country. It was not possible to draw any final conclusions as to the reasons for the observed differences.
    Matched MeSH terms: In Vitro Techniques
  12. Cheong WH, Warren M, Omar AH, Mahadevan S
    Science, 1965 Dec 03;150(3701):1314-5.
    PMID: 5857000
    The mosquito Anopheles balabacensis balabacensis has been identified as a natural vector of at least two species of simian malaria in the monsoon forests of the northern Malay States. This mosquito is also a serious vector of human malaria from Viet Nam to northern Malaya. This is the first report of a mosquito which transmits both human and simian malaria in nature.
    Matched MeSH terms: In Vitro Techniques
  13. Rudnick A
    J Med Entomol, 1965 Jun;2(2):203-8.
    PMID: 5827577
    Matched MeSH terms: In Vitro Techniques
  14. Balasingam E
    Med J Malaya, 1965 Sep;20(1):68-9.
    PMID: 4221427
    Matched MeSH terms: In Vitro Techniques
  15. Lowe CY
    Med J Malaya, 1965 Sep;20(1):56-7.
    PMID: 4221417
    Matched MeSH terms: In Vitro Techniques
  16. Warren M, Coatney GR, Skinner JC
    J Parasitol, 1966 Feb;52(1):9-13.
    PMID: 5910463
    Matched MeSH terms: In Vitro Techniques
  17. Nakanishi K, Sasaki S, Kiang AK, Goh J, Kakisawa H, Ohashi M, et al.
    Chem Pharm Bull (Tokyo), 1965 Jul;13(7):882-90.
    PMID: 5867816
    Matched MeSH terms: In Vitro Techniques
  18. Dalzell O, Mohd Ariffin S, Patrick CJ, Hardiman R, Manton DJ, Parashos P, et al.
    Eur Arch Paediatr Dent, 2021 Oct;22(5):911-927.
    PMID: 34146251 DOI: 10.1007/s40368-021-00641-2
    PURPOSE: Pulpectomy may be indicated in restorable primary teeth exhibiting irreversible pulpitis or pulpal necrosis. The purpose of this study was to compare the cleaning and shaping efficacy of NiTi systems (Reciproc® Blue and MTwo®) with manual stainless-steel instrumentation in primary molars using micro-CT analysis.

    METHODS: Fifty-seven maxillary second primary molars were scanned using micro-CT. Teeth with three divergent roots were divided randomly (n = 15) according to instrument type (K file, MTwo®, and Reciproc® Blue). Teeth with root fusion were instrumented manually as a separate group (n = 12). Pre- and post-instrumentation micro-CT images were superimposed, and the instrumentation area (IA) and procedural complications were recorded.

    RESULTS: No statistically significant differences in IA between file systems was observed in the non-fused teeth. The mean IA of fused roots was significantly lower than in the non-fused distobuccal (p = 0.003) and palatal (p  60%) occurred in both non-fused and fused primary teeth with fewer procedural complications observed after manual instrumentation.

    Matched MeSH terms: In Vitro Techniques
  19. Razavi M, Nyamathulla S, Karimian H, Noordin MI
    Drug Des Devel Ther, 2014;8:1315-29.
    PMID: 25246773 DOI: 10.2147/DDDT.S68517
    This study aimed to develop hydrophilic, gastroretentive matrix tablets of famotidine with good floating and swelling properties. A novel gastroretentive drug delivery formulation was designed using salep, also known as salepi, a flour obtained from grinding dried palmate tubers of Orchis morio var mascula (Orchidaceae family). The main polysaccharide content of salep is glucomannan, highly soluble in cold and hot water, which forms a viscous solution. Salep was characterized for physicochemical properties, thermal stability, chemical interaction, and surface morphology using X-ray diffraction analysis, differential scanning calorimetry, Fourier transform infrared spectroscopy, and scanning electron microscopy. Ten different formulations (S1-S10) were prepared using famotidine to salep ratios from 1:0.5 to 1:5. Results demonstrated that all formulations were able to sustain the drug release for more than 24 hours. The S5 formulation, with a famotidine to salep ratio of 1:2.5, had the shortest floating lag time of 35 seconds and 100% drug release within 24 hours. The dissolution data were fitted into popular mathematical models to assess the mechanism of drug release. S5 showed Zero order release (R=0.9746) with Higuchi diffusion (R=0.9428). We conclude that salep, a novel polymer, can be used in controlled release formulations to sustain release for 24 hours, due to inherent swelling and gelling properties.
    Matched MeSH terms: In Vitro Techniques
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