Displaying publications 1 - 20 of 54 in total

  1. Arumugam S, Yew HZ, Baharin SA, Qamaruz Zaman J, Muchtar A, Kanagasingam S
    Aust Endod J, 2021 Dec;47(3):520-530.
    PMID: 33956372 DOI: 10.1111/aej.12516
    This study aimed to evaluate and compare the frequency of microcracks and its severity at different root canal dentin locations, after preparation with thermomechanically heat-treated engine-driven nickel-titanium instruments. Forty mandibular premolars were assigned to four experimental groups (n = 10): ProTaper Next, ProTaper Gold, WaveOne Gold and Reciproc Blue. After pre-instrumentation micro-computed tomography scans, the root canals were prepared to size 25. Following post-instrumentation scans, pre- and post-instrumentation scanned images were analysed for the presence and extent of dentinal defects. A total of 56 500 cross-sectional images were obtained, showing that less than 2.3% with pre-existing dentinal microcracks. No new microcracks were identified during the post-instrumentation analyses. No significant association was found between the types of dentinal defects, file motions and sequences. Thermomechanically heat-treated rotary files did not induce the formation of new microcracks. There was also no association between the kinematic motions and sequences of the rotary instruments to the types of dentinal defects.
    Matched MeSH terms: X-Ray Microtomography
  2. Alyessary AS, Yap AUJ, Othman SA, Ibrahim N, Rahman MT, Radzi Z
    Am J Orthod Dentofacial Orthop, 2018 Aug;154(2):260-269.
    PMID: 30075928 DOI: 10.1016/j.ajodo.2017.11.031
    INTRODUCTION: In this study, we evaluated the effect of bone-borne accelerated expansion protocols on sutural separation and sutural bone modeling using a microcomputed tomography system. We also determined the optimum instant sutural expansion possible without disruption of bone modeling.

    METHODS: Sixteen New Zealand white rabbits, 20 to 24 weeks old, were randomly divided into 4 experimental groups. Modified hyrax expanders were placed across their interfrontal sutures and secured with miniscrew implants located bilaterally in the frontal bone. The hyrax appliances were activated as follows: group 1 (control), 0.5-mm per day expansion for 12 days; group 2, 1-mm instant expansion followed by 0.5 mm per day for 10 days; group 3, 2.5-mm instant expansion followed by 0.5 mm per day for 7 days, and group 4, 4-mm instant expansion followed by 0.5 mm per day for 4 days. After 6 weeks of retention, sutural separation and sutural bone modeling were assessed by microcomputed tomography and quantified. Statistical analysis was performed using Kruskal Wallis and Mann-Whitney U tests and the Spearman rho correlation (P <0.05).

    RESULTS: Median amounts of sutural separation ranged from 2.84 to 4.41 mm for groups 1 and 4, respectively. Median bone volume fraction ranged from 59.96% to 69.15% for groups 4 and 3, respectively. A significant correlation (r = 0.970; P <0.01) was observed between the amounts of instant expansion and sutural separation.

    CONCLUSIONS: Pending histologic verifications, our findings suggest that the protocol involving 2.5 mm of instant expansion followed by 0.5 mm per day for 7 days is optimal for accelerated sutural expansion. When 4 mm of instant expansion was used, the sutural bone volume fraction was decreased.

    Matched MeSH terms: X-Ray Microtomography*
  3. Ibrahim N, Parsa A, Hassan B, van der Stelt P, Rahmat RA, Ismail SM, et al.
    BMC Oral Health, 2021 05 08;21(1):249.
    PMID: 33964918 DOI: 10.1186/s12903-021-01595-z
    BACKGROUND: The aim of this study was to compare the trabecular bone microstructures of anterior and posterior edentulous regions of human mandible using cone-beam computed tomography (CBCT) and micro computed tomography (µCT).

    METHODS: Twenty volumes of interests consisting of six anterior and fourteen posterior edentulous regions were obtained from human mandibular cadavers. A CBCT system with a resolution of 80 µm (3D Accuitomo 170, J. Morita, Kyoto, Japan) and a µCT system with a resolution of 35 µm (SkyScan 1173, Kontich, Belgium) were used to scan the mandibles. Three structural parameters namely, trabecular number (Tb.N), trabecular thickness (Tb.Th), and trabecular separation (Tb.Sp) were analysed using CTAn software (v 1.11, SkyScan, Kontich, Belgium). For each system, the measurements obtained from anterior and posterior regions were tested using independent sample t-test. Subsequently, all measurements between systems were tested using paired t-test.

    RESULTS: In CBCT, all parameters of the anterior and posterior mandible showed no significant differences (p > 0.05). However, µCT showed a significant different of Tb.Th (p = 0.023) between anterior and posterior region. Regardless of regions, the measurements obtained using both imaging systems were significantly different (p ≤ 0.021) for Tb.Th and Tb.N.

    CONCLUSIONS: The current study demonstrated that only the variation of Tb.Th between anterior and posterior edentulous region of mandible can be detected using µCT. In addition, CBCT is less feasible than µCT in assessing trabecular bone microstructures at both regions.

    Matched MeSH terms: X-Ray Microtomography
  4. Nur Aliah NA, Heo CC, Noor Shafini M, Mohd Hafizi M
    Trop Biomed, 2019 Sep 01;36(3):640-653.
    PMID: 33597486
    Accurate estimation of the minimum post-mortem interval (minPMI) is important in the investigation of forensic cases. Various thanatological methods are being used to estimate this interval. However, entomology approach is the most reliable method for this minPMI estimation especially when death has occurred over 72 hours and involved insects or other arthropods evidence at the death scene. The current methods of age estimation are daunting and destructive especially when dealing with pupal stage. The aims of this study were to characterize the morphological changes during intra-puparial period of Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae) and their relation with minPMI estimation by using a high resolution micro-Computed Tomography (micro-CT). Gravid C. megacephala were collected from a rural area in Sungai Buloh, Selangor and cultured in the laboratory at 23.83±0.25°C with light: dark hour of 12:12 to initiate oviposition. The resulting larvae were reared until pupal stage. A pupa was collected at first (24 hours), second (48 hours), third (72 hours), and fourth quarter (96 hours) of the intra-puparial period. The pupal samples were placed directly into 70% ethanol for preservation. Micro-CT scanning was employed to acquire microstructural information following pupal sample staining for contrast enhancement. Eight age-informative internal morphological landmarks were mapped from the micro-CT scanning. The present study enhanced the potential value of micro-CT for the estimation of minPMI based on the internal morphological changes of C. megacephala pupae. This novel method is a promising tool for improving medico-legal investigations in forensic entomology.
    Matched MeSH terms: X-Ray Microtomography
  5. Effendy NM, Khamis MF, Shuid AN
    Curr Drug Targets, 2013 Dec;14(13):1542-51.
    PMID: 24010967
    Bone quality assessment is important in assessments of potential agents for the prevention and treatment of osteoporosis. Bone density, microarchitecture and strength are important determinants in osteoporotic study which are widely studied using Dual-Energy X-ray Absorptiometry (DXA), histomorphometry and radiological imaging techniques. In recent years, high resolution micro-CT has become feasible for in vitro or in vivo evaluation of bone architecture. Three-dimensional images of micro-CT reflected high correlations with the conventional histomorphometry and DXA. In comparison to other imaging techniques, micro-CT is the most effective tool in detecting early bone changes for fracture prediction and assessments of potential anti-osteoporotic agents. It is crucial to define an ideal setting with safe radiation doses and appropriate methods for image reconstruction and segmentation to obtain high resolution images. Micro-CT evaluation provides a better insight of bone structure as well as non-metric parameters such as connectivity density, structural model index (SMI) and degree of anisotropy (DA). This non- invasive imaging technique is also equipped with finite element analysis for evaluation of bone biomechanical strength. Micro-CT allows a compressive understanding of the relationships between bone density, microarchitecture and strength which is fundamental to development of pharmacological interventions.
    Matched MeSH terms: X-Ray Microtomography*
  6. Ahmed HM, Khamis MF, Gutmann JL
    Scanning, 2016 Nov;38(6):554-557.
    PMID: 26751249 DOI: 10.1002/sca.21299
    The root and root canal morphology of deciduous molars shows considerable variations. Consequently, a comprehensive understanding of the normal and unusual root and root canal configuration types in deciduous teeth is of prime importance. The purpose of this report is to describe a rare anatomical variation in a double-rooted maxillary deciduous molar examined by the dental operating microscope and micro-computed tomography. SCANNING 38:554-557, 2016. © 2016 Wiley Periodicals, Inc.
    Matched MeSH terms: X-Ray Microtomography/methods*
  7. Alavi R, Akbarzadeh AH, Hermawan H
    J Mech Behav Biomed Mater, 2021 05;117:104413.
    PMID: 33640846 DOI: 10.1016/j.jmbbm.2021.104413
    In-depth analyses of post-corrosion mechanical properties and architecture of open cell iron foams with hollow struts as absorbable bone scaffolds were carried out. Variations in the architectural features of the foams after 14 days of immersion in a Hanks' solution were investigated using micro-computed tomography and scanning electron microscope images. Finite element Kelvin foam model was developed, and the numerical modeling and experimental results were compared against each other. It was observed that the iron foam samples were mostly corroded in the periphery regions. Except for quasi-elastic gradient, other mechanical properties (i.e. compressive strength, yield strength and energy absorbability) decreased monotonically with immersion time. Presence of adherent corrosion products enhanced the load-bearing capacity of the open cell iron foams at small strains. The finite element prediction for the quasi-elastic response of the 14-day corroded foam was in an agreement with the experimental results. This study highlights the importance of considering corrosion mechanism when designing absorbable scaffolds; this is indispensable to offer desirable mechanical properties in porous materials during degradation in a biological environment.
    Matched MeSH terms: X-Ray Microtomography
  8. Khan AS, Ur Rehman S, Ahmad S, AlMaimouni YK, Alzamil MAS, Dummer PMH
    Int Endod J, 2021 Oct;54(10):1819-1839.
    PMID: 34196006 DOI: 10.1111/iej.13595
    AIM: The International Endodontic Journal (IEJ) has served as a platform for research and clinical practice in Endodontics since 1967. This study provides a bibliographic analysis and overview of the publications that have appeared in the IEJ from 1967 to 2020.

    METHODOLOGY: A literature search was performed in Elsevier's Scopus database to locate all the publications of the International Endodontic Journal. Various bibliometric software packages including the open-source visualization software Gephi and Biblioshiny (version 2.0) were employed for data visualization and analysis.

    RESULTS: A total of 3739 records with citation and bibliographic details were selected and retrieved to allow a bibliometric analysis to be performed. The bibliometric analysis indicates that the IEJ has grown both in terms of productivity and influence. Over time, the journal has been associated with an increase in the number of manuscripts published and the citations they have attracted, but with minor downward fluctuations in citations in the last few years. Bibliographic coupling of the IEJ articles revealed that the major research themes published in the journal include 'endodontics', 'root canal treatment', 'calcium hydroxide', 'apical periodontitis', 'mineral trioxide aggregate', 'microbiology', 'cyclic fatigue', 'cone-beam computed tomography' and 'micro-computed tomography'. Authors affiliated to institutions in the UK were the major contributors to the journal and were linked with other countries such as Brazil, USA and Malaysia. The largest number of publications were from the University of São Paulo, Brazil.

    CONCLUSION: The IEJ is one of the leading journals in Endodontology and has been providing a platform for innovative research and clinical reports for more than 50 years. Publications have been associated with a wide range of authors, institutions and countries around the world.

    Matched MeSH terms: X-Ray Microtomography
  9. Goh CF, O'Flynn D, Speller R, Lane ME
    Micron, 2021 06;145:103045.
    PMID: 33689970 DOI: 10.1016/j.micron.2021.103045
    Drug crystallisation in the skin is recognised as a significant problem in topical and transdermal drug delivery. Our recent investigations provided new evidence of drug crystallisation in the skin, however, confirming the precise location of crystals remains challenging. Of note, most approaches used have required disruption of the membrane by tape stripping, with crystal detection limited to the superficial skin layers. Hence, a non-destructive method for complete spatial resolution of crystallised drug in skin is still lacking. In this communication, we report the application of X-ray micro-computed tomography (microCT) to examine drug crystallisation in mammalian skin ex vivo. Permeation studies of a saturated solution of diclofenac sodium were conducted in porcine skin; subsequently, tissue samples were scanned using microCT to generate 2D and 3D maps. A layer of drug crystals was observed on the skin surface; microCT maps also confirmed the distribution of drug crystals up to a skin depth of 0.2 - 0.3 mm. MicroCT also allowed the identification of drug crystallisation as a distinct and confirmed event in the skin and as an extension from drug crystals formed on the skin. These preliminary results confirm the potential of microCT to study this important phenomenon in topical and transdermal drug delivery.
    Matched MeSH terms: X-Ray Microtomography
  10. Sulaiman SZS, Tan WM, Radzi R, Shafie INF, Ajat M, Mansor R, et al.
    J Orthop Surg Res, 2021 Nov 08;16(1):663.
    PMID: 34749769 DOI: 10.1186/s13018-021-02781-z
    BACKGROUND: Osteoarthritis (OA) is a multifaceted condition that affects both the subchondral bones and the articular cartilage. Animal models are widely used as an effective supplement and simulation for human OA studies in investigating disease mechanisms and pathophysiology. This study is aimed to evaluate the temporal changes of bone and cartilage in surgically and chemically induced osteoarthritis using micro-computed tomography and histology.

    METHODS: Thirty rabbits underwent either anterior cruciate ligament transection (ACLT) procedure or injected intraarticularly with monosodium iodoacetate (MIA, 8 mg) at the right knee joint. The subchondral bones were scanned via micro-CT, and articular cartilage was assessed histologically at 4-, 8- and 12-week post-induction.

    RESULTS: Based on bone micro-architecture parameters, the surgically induced group revealed bone remodelling processes, indicated by increase bone volume, thickening of trabeculae, reduced trabecular separation and reduced porosity. On the other hand, the chemically induced group showed active bone resorption processes depicted by decrease bone volume, thinning of trabeculae, increased separation of trabecular and increased porosity consistently until week 12. Histologically, the chemically induced group showed more severe articular cartilage damage compared to the surgically induced group.

    CONCLUSIONS: It can be concluded that in the ACLT group, subchondral bone remodelling precedes articular cartilage damage and vice versa in the MIA group. The findings revealed distinct pathogenic pathways for both induction methods, providing insight into tailored therapeutic strategies, as well as disease progression and treatment outcomes monitoring.

    Matched MeSH terms: X-Ray Microtomography
  11. Yunos MA, Hussain SA, Yusoff HM, Abdullah J
    Appl Radiat Isot, 2014 Sep;91:57-61.
    PMID: 24907683 DOI: 10.1016/j.apradiso.2014.05.015
    Radioactive particle tracking (RPT) has emerged as a promising and versatile technique that can provide rich information about a variety of multiphase flow systems. However, RPT is not an off-the-shelf technique, and thus, users must customize RPT for their applications. This paper presents a simple procedure for preparing radioactive tracer particles created via irradiation with neutrons from the TRIGA Mark II research reactor. The present study focuses on the performance evaluation of encapsulated gold and scandium particles for applications as individual radioactive tracer particles using qualitative and quantitative neutron activation analysis (NAA) and an X-ray microcomputed tomography (X-ray Micro-CT) scanner installed at the Malaysian Nuclear Agency.
    Matched MeSH terms: X-Ray Microtomography
  12. Abdul Razak HR, Shaffiq Said Rahmat SM, Md Saad WM
    Quant Imaging Med Surg, 2013 Oct;3(5):256-61.
    PMID: 24273743 DOI: 10.3978/j.issn.2223-4292.2013.10.04
    The study aimed to investigate the effects of different tube potentials and concentrations of iodinated contrast media (CM) on the image enhancement, contrast-to-noise ratio (CNR) and noise in micro-computed tomography (µCT) images. A phantom containing of five polyethylene tube was filled with 2 mL of deionized water and iodinated CM (Omnipaque 300 mgI/mL) at four different concentrations: 5, 10, 15, and 20 mol/L, respectively. The phantom was scanned with a µCT machine (SkyScan 1176) using various tube potentials: 40, 50, 60, 70, 80, and 90 kVp, a fixed tube current; 100 µA, and filtration of 0.2 mm aluminum (Al). The percentage difference of image enhancement, CNR and noise of all images, acquired at different kVps and concentrations, were calculated. The image enhancement, CNR and noise curves with respect to tube potential and concentration were plotted and analysed. The highest image enhancement was found at the lowest tube potential of 40 kVp. At this kVp setting, the percentage difference of image enhancement [Hounsfield Unit (HU) of 20 mol/L iodine concentration over HU of deionized water] was 43%. By increasing the tube potential, it resulted with the reduction of HU, where only 17.5% different were noticed for 90 kVp. Across all iodine concentrations (5-20 M), CNR peaked at 80 kVp and then these values showed a slight decreasing pattern, which might be due insufficient tube current compensation. The percentage difference of image noise obtained at 40 and 90 kVp was 72.4%. Lower tube potential setting results in higher image enhancement (HU) in conjunction with increasing concentration of iodinated CM. Overall, the tube potential increment will substantially improve CNR and reduce image noise.
    Matched MeSH terms: X-Ray Microtomography
  13. Ahmed HMA, Ibrahim N, Mohamad NS, Nambiar P, Muhammad RF, Yusoff M, et al.
    Int Endod J, 2021 Jul;54(7):1056-1082.
    PMID: 33527452 DOI: 10.1111/iej.13486
    Adequate knowledge and accurate characterization of root and canal anatomy is an essential prerequisite for successful root canal treatment and endodontic surgery. Over the years, an ever-increasing body of knowledge related to root and canal anatomy of the human dentition has accumulated. To correct deficiencies in existing systems, a new coding system for classifying root and canal morphology, accessory canals and anomalies has been introduced. In recent years, micro-computed tomography (micro-CT) and cone beam computed tomography (CBCT) have been used extensively to study the details of root and canal anatomy in extracted teeth and within clinical settings. This review aims to discuss the application of the new coding system in studies using micro-CT and CBCT, provide a detailed guide for appropriate characterization of root and canal anatomy and to discuss several controversial issues that may appear as potential limitations for proper characterization of roots and canals.
    Matched MeSH terms: X-Ray Microtomography
  14. Gan SC, Fok ASL, Sedky RA, Sukumaran P, Chew HP
    Dent Mater, 2020 11;36(11):1379-1387.
    PMID: 32907752 DOI: 10.1016/j.dental.2020.08.003
    OBJECTIVE: The aim of this research was to determine the association between sorptivity of water and the state of mineralization in carious enamel of different stages of severity.

    METHODS: As a preliminary work, water droplets of 1.5 μL were placed on the surfaces of hydroxyapatite (HA) discs of different densities. The water droplet profile was dynamically recorded every second over a period of 10 s using a contact angle meter to determine the relationship between sorptivity and density. To measure and calculate sorptivity on enamel surfaces, varnish was painted on the labial surface of 96 extracted caries-free human teeth, leaving two 1.4 ± 0.1 mm diameter circular exposed test sites. The specimens were randomly divided into 6 groups (n = 16) and subjected to 0(G0), 7(G7), 14(G14), 21(G21), 28(G28) and 35(G35) days of pH cycling, respectively. A 0.7 μL water droplet was placed on each exposed site and Optical Coherence Tomography was used to measure its height every 10 seconds for 2 min. Sorptivity was computed by considering sorption equations and Washburn's analysis of capillary kinetics and correction for evaporation was also performed. Micro-Computed Tomography scans of the specimens were obtained and delta Z (ΔZ) is the parameter used to measure mineral loss. ΔZ at 10 μm (ΔZ10) and 50 μm (ΔZ50) from the surface were calculated. One-way ANOVA and Post-hoc Tukey tests were used to compare sorptivity between groups and bivariate correlations were used to analyze the association between sorptivity and ΔZ.

    RESULTS: Sorptivity was found to be inversely and linearly correlated with HA density with R2 value of 0.95. With enamel, there is a general trend of increase in mean sorptivity from G0 to G35, except for a decrease in G21. The same trends were observed for both ΔZ10 and ΔZ50. The decrease in sorptivity in G21 coincided with the presence of a surface hypermineralized layer in G21 samples. Post-hoc Tukey showed significant differences in mean sorptivity between G0 and G14, G0 and G21 as well as G14 and G21. Post-hoc Dunnett's T3 showed significant differences for ΔZ10 between G0 and G14 as well as G14 and G21. Significant correlation between mean sorptivity and ΔZ10 was detected with Pearson correlation coefficient of 0.461. For ΔZ50, post-hoc Tukey showed significant differences between G0 and G14 but no significant difference was detected between G14 and G21. No correlations were detected between mean sorptivity and ΔZ50.

    SIGNIFICANCE: Sorptivity was found to be inversely and linearly correlated with HA density with R2 value of 0.95. With enamel, there is a general trend of increase in mean sorptivity from G0 to G35, except for a decrease in G21. The same trends were observed for both ΔZ10 and ΔZ50. The decrease in sorptivity in G21 coincided with the presence of a surface hypermineralized layer in G21 samples.

    Matched MeSH terms: X-Ray Microtomography
  15. Khairiah Yazid @ Khalid, Roslan Yahya, Nadira Kamarudini, Mohd. Zaid Abdullah, Mohd Ashhar Khalid, Abdul Aziz Mohamed
    Detection and analysis of resin is particularly significant since the commercial value of agarwood is related to the quantity of resins that are present. This article explores the potential of a scanning electron microscope in combination with new non-destructive 3D visualization technique, X-ray micro-computed tomography, as imaging tools to visualize micro-structure resin in agarwood. These techniques were used to compare two samples of agarwood chips: high grade and low grade. From the results, it can be concluded that a wood cell filled with resin deposit have a higher attenuation. It can be shown that the combination of scanning electron microscopy and micro-CT can offer high resolution images concerning the localization and structure of resin inside Agarwood. While the second allows the 3D investigation of internal structure of agarwood, the first technique can provide details 2D morphological information. These imaging techniques, although sophisticated can be used for standard development especially in grading of agarwoodlbr commercial activities.
    Matched MeSH terms: X-Ray Microtomography
  16. Mok PL, Leow SN, Koh AE, Mohd Nizam HH, Ding SL, Luu C, et al.
    Int J Mol Sci, 2017 Feb 08;18(2).
    PMID: 28208719 DOI: 10.3390/ijms18020345
    Mesenchymal stem cells are widely used in many pre-clinical and clinical settings. Despite advances in molecular technology; the migration and homing activities of these cells in in vivo systems are not well understood. Labelling mesenchymal stem cells with gold nanoparticles has no cytotoxic effect and may offer suitable indications for stem cell tracking. Here, we report a simple protocol to label mesenchymal stem cells using 80 nm gold nanoparticles. Once the cells and particles were incubated together for 24 h, the labelled products were injected into the rat subretinal layer. Micro-computed tomography was then conducted on the 15th and 30th day post-injection to track the movement of these cells, as visualized by an area of hyperdensity from the coronal section images of the rat head. In addition, we confirmed the cellular uptake of the gold nanoparticles by the mesenchymal stem cells using transmission electron microscopy. As opposed to other methods, the current protocol provides a simple, less labour-intensive and more efficient labelling mechanism for real-time cell tracking. Finally, we discuss the potential manipulations of gold nanoparticles in stem cells for cell replacement and cancer therapy in ocular disorders or diseases.
    Matched MeSH terms: X-Ray Microtomography
  17. Mohd Ariffin S, Dalzell O, Hardiman R, Manton DJ, Parashos P, Rajan S
    Eur Arch Paediatr Dent, 2020 Aug;21(4):519-525.
    PMID: 32100200 DOI: 10.1007/s40368-020-00515-z
    AIM: Successful endodontic treatment of primary teeth requires comprehensive knowledge and understanding of root canal morphology. The purpose of this study was to investigate the root canal configurations of primary maxillary second molars using micro-computed tomography.

    METHODS: Extracted human primary maxillary second molars (n = 57) were scanned using micro-computed tomography and reconstructed to produce three-dimensional models. Each root canal system was analysed qualitatively according to Vertucci's classification.

    RESULTS: 22.8% (n = 13) of the sample presented with the fusion of the disto-buccal and palatal roots; of these, Type V was the most prevalent classification. For teeth with three separate roots (n = 44), the most common root canal type was Type 1 for the palatal canal (100%) and disto-buccal canal (77.3%) and Type V for the mesio-buccal canal (36.4%). Overall, 7% (n = 4) of mesio-buccal canals were 'unclassifiable'.

    CONCLUSION: The root canal systems of primary maxillary second molars were not only complex but had a range of configurations that may contribute to unfavourable clinical outcomes after endodontic treatment.

    Matched MeSH terms: X-Ray Microtomography
  18. Dalzell O, Mohd Ariffin S, Patrick CJ, Hardiman R, Manton DJ, Parashos P, et al.
    Eur Arch Paediatr Dent, 2021 Oct;22(5):911-927.
    PMID: 34146251 DOI: 10.1007/s40368-021-00641-2
    PURPOSE: Pulpectomy may be indicated in restorable primary teeth exhibiting irreversible pulpitis or pulpal necrosis. The purpose of this study was to compare the cleaning and shaping efficacy of NiTi systems (Reciproc® Blue and MTwo®) with manual stainless-steel instrumentation in primary molars using micro-CT analysis.

    METHODS: Fifty-seven maxillary second primary molars were scanned using micro-CT. Teeth with three divergent roots were divided randomly (n = 15) according to instrument type (K file, MTwo®, and Reciproc® Blue). Teeth with root fusion were instrumented manually as a separate group (n = 12). Pre- and post-instrumentation micro-CT images were superimposed, and the instrumentation area (IA) and procedural complications were recorded.

    RESULTS: No statistically significant differences in IA between file systems was observed in the non-fused teeth. The mean IA of fused roots was significantly lower than in the non-fused distobuccal (p = 0.003) and palatal (p  60%) occurred in both non-fused and fused primary teeth with fewer procedural complications observed after manual instrumentation.

    Matched MeSH terms: X-Ray Microtomography
  19. Ahmed HMA, Rossi-Fedele G
    Eur Endod J, 2020 12;5(3):159-176.
    PMID: 33353923 DOI: 10.14744/eej.2020.88942
    OBJECTIVE: Consistent reporting of publications in a given topic is essential. This systematic review aimed to identify and evaluate the reporting items in previous publications related to root canal anatomy in major Endodontic journals.

    METHODS: A systematic review was undertaken following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. A comprehensive literature search was performed by 2 independent reviewers using a customized search strategy in major Endodontic journals through Scopus until November 2019. Studies investigating root and canal anatomy were included. The selected publications were divided into 7 categories according to the study design: micro-computed tomography (microCT) and cone-beam computed tomography (CBCT) experimental studies (extracted teeth), CBCT and 2D clinical studies, CBCT and 2D case reports in addition to others (i.e. staining and clearing method and root sectioning). The selected studies were evaluated according to three domains: 1) Criteria for study sample selection; 2) Criteria for methodological procedures and 3) Criteria for detection and evaluation.

    RESULTS: After the removal of duplicated and irrelevant papers, 137 articles were included. Results showed that microCT studies reported accurately the tooth type, number of teeth, classifications used, qualitative and/or quantitative analysis (if required) and the evaluation process. However, sample size calculation, calibration, and reproducibility were not reported in the majority of microCT studies. CBCT clinical studies presented information for the type of study, inclusion/exclusion criteria, number of patients, tooth type, and number of teeth. However, the majority did not report sample size calculation and calibration of examiners. Radiographic exposure descriptions and classifications used were not reported adequately in CBCT and 2D case reports. Sample size calculation, calibration and reproducibility were not reported in staining and clearing method.

    CONCLUSION: Despite accurate presentation of certain items, there is considerable inconsistent reporting of root and canal morphology regardless of the type of study and experimental procedure used. The PROUD checklist protocol presented in this systematic review aims to provide an accurate description of root canal anatomy in experimental, clinical, and case report publications.

    Matched MeSH terms: X-Ray Microtomography
  20. Omar FN, Hafid HS, Samsu Baharuddin A, Mohammed MAP, Abdullah J
    Planta, 2017 Sep;246(3):567-577.
    PMID: 28620814 DOI: 10.1007/s00425-017-2717-5
    MAIN CONCLUSION: X-ray microtomography results revealed that delignification process damaged the oil palm fibers, which correlated well with reduction of lignin components and increase of the phenolic content. Biodegradation investigation of natural fibers normally focuses on physico-chemical analysis, with less emphasis on physical aspect like fiber structures affect from microbial activity. In this work, the performance of Pycnoporus sanguineus to delignify oil palm empty fruit bunch fibers through solid-state fermentation utilizing various ratio of POME sludge was reported. In addition to tensile testing, physico-chemical and X-ray microtomography (µ-CT) analyses on the oil palm fibers were conducted to determine the effectiveness of the degradation process. The best ratio of fiber to fungi (60:40) was chosen based on the highest lignin loss and total phenolic content values and further investigation was performed to obtain fermentation kinetics data of both laccase and manganese peroxidase. µ-CT results revealed that delignification process damaged the pre-treated and untreated fibers structure, as evident from volume reduction after degradation process. This is correlated with reduction of lignin component and increase of the phenolic content, as well as lower stress-strain curves of the pre-treated fibers compared to the untreated ones (from tensile testing). It is suggested that P. sanguineus preferred to consume the outer layer of the fiber, before it penetrates through the cellular structure of the inner fiber.
    Matched MeSH terms: X-Ray Microtomography
Contact Us

Please provide feedback to Administrator (afdal@afpm.org.my)

External Links