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  1. Extraction and characterization of gelatin from different marine fish species in Malaysia
    Irwandi, J., Faridayanti, S., Mohamed, E.S.M., Hamzah, M.S., Torla, H.H., Che Man, Y.B.
    International Food Research Journal, 2009;16(3):381-389.
    MyJurnal
    Gelatins from the skin of four local marine fish, namely “kerapu” (Epinephelus sexfasciatus), “jenahak” (Lutjianus argentimaculatus), “kembung” (Rastrelliger kanagurta), and “kerisi” (Pristipomodes typus) have been successfully extracted by acid extraction. Results characterization showed that the fish gelatins were comparable to the fish gelatins from other fish species previously reported. They appeared snowy white in color with crystal-like and light texture. The gelatine extracted from “kerapu” had the strongest fishy odor, followed by the gelatines derived from “jenahak”, “kembung” and “kerisi”. In terms of bloom strength, the gelatin extracted from “kerapu” was found to be the strongest one compared to others, with the bloom value of more than 2000 g. The gelatins developed in this study contained almost all essential amino acids, with glycine being the most predominant one.
    Matched MeSH terms: Amino Acids, Essential
  2. Fulltext Macronutrients content of red seaweed kappaphycus alvarezii and kappaphycus striatum
    Chan, Kok Meng, Farah Diyana Ariffin, Aminah Abdullah, Shahrul Hisham Zainal Ariffin
    Jurnal Sains Kesihatan Malaysia, 2017;15(2):19-27.
    MyJurnal
    Seaweed contains various nutrients that has the potential to be a source of nutritious food, but only a few studies done on
    the red seaweeds in Malaysia. Therefore, this study was conducted to determine the macronutrients content, amino acid
    profile and fatty acid component in Kappaphycus alvarezii and Kappaphycus striatum. The study found that the range
    of moisture, fat, ash, protein, fiber and carbohydrates content for both red seaweeds were 6.9% - 7.3%, 0.5% - 2.6%,
    29.4% - 30.9%, 2.5% - 5.7% , 5.3% - 5.5% and 50.1% - 53.3% respectively. A total of 16 amino acids were identified
    in which the essential amino acid for K. alvarezii and K. striatum were 41.11% and 36.15% respectively. A total of 34
    fatty acids were identified in which the content of saturated fatty acids (SFA) was the highest (42.7% - 72.8%), followed
    by mono-unsaturated fatty acid (MUFA) (13.8% - 36.2%) and polyunsaturated fatty acids (PUFAs) was the lowest (13.5%
    - 21.2%). In conclusion, this study suggest that K. alvarezii and K. striatum are potentially be used as raw materials or
    food ingredients to improve the nutritional value of the human diet.
    Matched MeSH terms: Amino Acids, Essential
  3. Fulltext Novel essential amino acid-sulfanilamide hybrid as safe anti-ulcerogenic agent with anti-helicobacter pylori activity
    Awaad AS, Alafeefy AM, Alasmary FAS, El-Meligy RM, Zain ME, Alqasoumi SI
    Saudi Pharm J, 2017 Nov;25(7):967-971.
    PMID: 29158702 DOI: 10.1016/j.jsps.2017.02.012
    A novel and safe essential amino acid (Leucine) incorporating sulfanilamide was synthesized, and evaluated for its anti-ulcerogenic activity and in vitro anti-Helicobacter pylori activity. The new molecule showed a dose dependent activity against absolute ethanol-induced ulcer in rats, it produced percent protection of control ulcer by 66.7 at dose 100 mg/kg. In addition it showed a potent anti-Helicobacter pylori activity in vitro against 7 clinically isolated strains. The minimum inhibitory concentration (MIC) ranged from 12.5 to 50 μg/ml. The preliminary safety studies and toxicity profile are optimistic and encouraging.
    Matched MeSH terms: Amino Acids, Essential
  4. Fulltext Effects of degree of hydrolysis on physicochemical properties of cobia (Rachycentron canadum) frame hydrolysate
    Amiza, M.A., Kong, Y.L., Faazaz, A.L.
    International Food Research Journal, 2012;19(1):199-206.
    MyJurnal
    The effect of degree of hydrolysis (DH) on the physicochemical properties of cobia frame hydrolysate was determined. Three levels of degree of hydrolysis of cobia frame hydrolysate were studied, which were 53%, 71% and 96%. After enzymatic hydrolysis using Alcalase®, the samples were spray-dried. Cobia hydrolysate powder samples were analyzed for their proximate analysis and physicochemical properties. The proximate analysis showed significant differences in fat and ash content only. DH96 hydrolysate showed desirable essential amino acid profile for human requirement except for methionine and isoleucine. The study found that cobia frame hydrolysate had good colour, emulsifying capacity and excellent foaming properties. However, there were no significant differences in water-holding capacity, oil-holding capacity and peptide solubility among the hydrolysate samples. This study suggested that cobia frame hydrolysate is a potential ingredient and foaming agent for food industry.
    Matched MeSH terms: Amino Acids, Essential
  5. Nutritional properties of spent duck surimi-like material prepared by conventional and acid-alkaline solubilization methods
    Nurkhoeriyati T, Huda N, Ahmad R
    Int J Food Sci Nutr, 2012 Jun;63(4):498-505.
    PMID: 22126368 DOI: 10.3109/09637486.2011.637902
    The nutritional properties of surimi-like materials produced from spent duck meat processed conventionally (CDS) and processed with acid and alkaline solubilization (ACDS and ALDS, respectively) were studied. The essential amino acids (EAAs) content was significantly higher (p amino acid score and EAA index than the CON. The saturated fatty acid compositions of the ACDS and ALDS samples were significantly lower (p 
    Matched MeSH terms: Amino Acids, Essential/analysis*
  6. Effects of low-protein diets on acute phase proteins and heat shock protein 70 responses, and growth performance in broiler chickens under heat stress condition
    Zulkifli I, Akmal AF, Soleimani AF, Hossain MA, Awad EA
    Poult Sci, 2018 Apr 01;97(4):1306-1314.
    PMID: 29381776 DOI: 10.3382/ps/pex436
    A study with a 4 × 2 factorial arrangement was conducted to investigate the effects of 4 dietary protein levels and 2 environmental conditions on acute phase proteins (APP), brain heat shock protein (HSP) 70 density, and growth performance of broiler chickens. Day-old broiler chicks (Cobb 500) were fed isocaloric diets but with various levels of crude protein (CP), namely, (1) 21.0 and 19.0% CP in starter and finisher diets, respectively (control), (2) 19.5 and 17.5% CP in starter and finisher diets, respectively (Diet A), (3) 18.0 and 16.0% CP in starter and finisher diets, respectively (Diet B), and (4) 16.5 and 14.5% CP in starter and finisher diets, respectively (Diet C). Equal numbers of birds from each diet were subjected to either 23±1°C throughout or 33±1°C for 6 h per d from 22 to 35 d of age. From d 1 to 21, feed intake (FI) and weight gain (WG) decreased linearly (P = 0.021 and P = 0.009, respectively), as CP level was reduced. During the heat treatment period (d 22 to 35), there were significant (P = 0.04) diet × heat treatment interactions for FCR. Diet had no effect on FCR among the unheated birds, but the ratio increased linearly (P = 0.007) as dietary CP level decreased. Irrespective of ambient temperature, there was a significant linear decrease in FI (P = 0.032) and WG (P < 0.001) as dietary CP level decreased. Low-CP diets improved the survivability of heat-stressed broilers when compared to those fed control diets. Low-CP diets linearly decreased (P < 0.01) APP (ovotransferrin and alpha-acid glycoprotein) responses. Both APP and HSP 70 reactions were elevated following heat treatment. In conclusion, feeding broilers with low-CP diets adversely affect the growth performance of broilers under heat stress condition. However, low-CP diets were beneficial in improving the survivability. Because APP are involved in the restoration of homeostasis, the adverse effect of low-CP diet on the synthesis of these proteins could be of concern.
    Matched MeSH terms: Amino Acids, Essential/administration & dosage; Amino Acids, Essential/metabolism*
  7. A Comprehensive Review on L-Asparaginase and Its Applications
    Batool T, Makky EA, Jalal M, Yusoff MM
    Appl Biochem Biotechnol, 2016 Mar;178(5):900-23.
    PMID: 26547852 DOI: 10.1007/s12010-015-1917-3
    L-asparaginase (LA) catalyzes the degradation of asparagine, an essential amino acid for leukemic cells, into ammonia and aspartate. Owing to its ability to inhibit protein biosynthesis in lymphoblasts, LA is used to treat acute lymphoblastic leukemia (ALL). Different isozymes of this enzyme have been isolated from a wide range of organisms, including plants and terrestrial and marine microorganisms. Pieces of information about the three-dimensional structure of L-asparaginase from Escherichia coli and Erwinia sp. have identified residues that are essential for catalytic activity. This review catalogues the major sources of L-asparaginase, the methods of its production through the solid state (SSF) and submerged (SmF) fermentation, purification, and characterization as well as its biological roles. In the same breath, this article explores both the past and present applications of this important enzyme and discusses its future prospects.
    Matched MeSH terms: Amino Acids, Essential
  8. Fulltext Untargeted and Targeted Metabolomic Profiling of Australian Indigenous Fruits
    Lim V, Gorji SG, Daygon VD, Fitzgerald M
    Metabolites, 2020 Mar 19;10(3).
    PMID: 32204361 DOI: 10.3390/metabo10030114
    Selected Australian native fruits such as Davidson's plum, finger lime and native pepperberry have been reported to demonstrate potent antioxidant activity. However, comprehensive metabolite profiling of these fruits is limited, therefore the compounds responsible are unknown, and further, the compounds of nutritional value in these native fruits are yet to be described. In this study, untargeted and targeted metabolomics were conducted using the three fruits, together with assays to determine their antioxidant activities. The results demonstrate that targeted free and hydrolysed protein amino acids exhibited high amounts of essential amino acids. Similarly, important minerals like potassium were detected in the fruit samples. In antioxidant activity, Davidson's plum reported the highest activity in ferric reducing power (FRAP), finger lime in antioxidant capacity (ABTS), and native pepperberry in free radical scavenging (DPPH) and phosphomolybdenum assay. The compounds responsible for the antioxidant activity were tentatively identified using untargeted GC×GC-TOFMS and UHPLC-QqQ-TOF-MS/MS metabolomics. A clear discrimination into three clusters of fruits was observed using principal component analysis (PCA) and partial least squares (PLS) analysis. The correlation study identified a number of compounds that provide the antioxidant activities. GC×GC-TOFMS detected potent aroma compounds of limonene, furfural, and 1-R-α-pinene. Based on the untargeted and targeted metabolomics, and antioxidant assays, the nutritional potential of these Australian bush fruits is considerable and supports these indigenous fruits in the nutraceutical industry as well as functional ingredients for the food industry, with such outcomes benefiting Indigenous Australian communities.
    Matched MeSH terms: Amino Acids, Essential
  9. Fulltext Proximate Analysis of Selected Macroalgal Species from the Persian Gulf as a Nutritional Resource
    Pirian K, Jeliani ZZ, Arman M, Sohrabipour J, Yousefzadi M
    Trop Life Sci Res, 2020 Apr;31(1):1-17.
    PMID: 32963708 DOI: 10.21315/tlsr2020.31.1.1
    Nowadays the exploration and utilisation of food and feed from marine origin is becoming more important with the increase of human population. Macroalgae are rich in nutritious compounds, which can directly be used in human and animal feed industries. The current study presents the screening of chemical components of eight macroalgae species, Sargassum boveanum, Sirophysalis trinodis, Hypnea caroides, Palisda perforata, Galaxaura rugosa, Caulerpa racemose, Caulerpa sertularioides and Bryopsis corticolans from the Persian Gulf. The results revealed that the eight studied algal species possess high protein (14.46% to 38.20%), lipid (1.27% to 9.13%) and ash (15.50% to 49.14%) contents. The fatty acids and amino acids profile showed the presence of essential fatty acids and amino acids with high nutritional value. Phaeophyta species, S. boveanum and S. trinodis, showed the highest value of ash content and polyunsaturated fatty acids while Chlorophyta species, C. racemose, C. sertularioides and B. corticolans, showed the highest level of lipid and protein contents. Rhodophyta species, G. rugosa and P. perforata, showed the highest essential amino acid content. In conclusion, this study demonstrates the potential of the studied marine species as a nutritional source for human and animal uses.
    Matched MeSH terms: Amino Acids, Essential
  10. Fulltext Preliminary studies on the evaluation of nutritional composition of unripe and ripe ‘Kundang’ fruits (Bouea macrophylla Griffith)
    Rajan, Nithiya Shanmuga, Bhat,Rajeev, Karim, A.A.
    International Food Research Journal, 2014;21(3):985-990.
    MyJurnal
    Unripe and ripe kundang fruits (Bouea macrophylla Griffith) is either consumed fresh or is cooked in Malaysia. In this study composition of unripe and ripe fruits (proximate, amino acids profile, minerals and heavy metal contents) were evaluated. Results obtained showed unripe kundang fruit to possess higher moisture, ash, crude lipid, crude fiber and crude protein contents than the ripe fruits. With regard to amino acid contents, unripe fruits had higher content of essential amino acids. The unripe and ripe fruits were found to be rich in essential minerals with potassium (K) to be in abundance. Heavy metals such as cadmium, nickel, mercury, lead and arsenic, were detected in trace amounts (< 5.0 mg/kg) in both unripe and ripe fruits. Through this investigation, it is concluded that both unripe and ripe fruits to posses’ adequate amount of nutritionally important compounds beneficial to human health and can be explored for commercial purposes.
    Matched MeSH terms: Amino Acids, Essential
  11. Fulltext Nutritional composition and amino acid profile of a sub-tropical red seaweed Gelidium pusillum collected from St. Martin’s Island, Bangladesh
    Siddique, M.A.M., Khan, M.S.K., Bhuiyan, M.K.A.
    International Food Research Journal, 2013;20(5):2287-2292.
    MyJurnal
    Nutritional fact study has prime importance to make the species edible and commercially viable to the food consumers. The proximate chemical composition and amino acid profile of Gelidium pusillum were studied to understand the nutritional status. The red seaweed Gelidium pusillum was rich in dietary fibre (24.74 ± 1.05%), lipid (2.16 ± 0.61%) and ash content (21.15 ± 0.74%). The mean protein content (11.31 ± 1.02% DW) was within the range of 10-47% for green and red seaweeds and this range was higher than Gracilaria cornea (5.47% DW), Gracilaria changgi (6.90% DW) and Eucheuma cottonii (9.76% DW). Gelidium pusillum was found to contained all the essential amino acids, which accounted for 52.08% of the total amino acids. Tyrosine (26.2 mg g-1 protein), methionine (15.8 mg g-1 protein) and Lysine (48.3 mg g-1 protein) were the limiting amino acid of Gelidium pusillum. However, the levels of other essential amino acids were above the FAO/WHO requirement pattern (EAA score ranged from 1.14 to 1.62). Aspartic and glutamic acids constituted a substantial amount of the total amino acids (24.68% of total amino acid). The result from this study suggested that Gelidium pusillum could be utilized as a healthy food item for human consumption.
    Matched MeSH terms: Amino Acids, Essential
  12. Fulltext Physicochemical properties of silver catfish (Pangasius sp.) frame hydrolysate
    Amiza, M.A., Ow, Y.W., Faazaz, A.L.
    International Food Research Journal, 2013;20(3):1255-1262.
    MyJurnal
    The physicochemical properties of silver catfish frame hydrolysate powder at three different degree of hydrolysis, DH43%, DH 55% and DH 68% were studied. The hydrolysates powder were obtained by hydrolysis using Alcalase®, centrifugation and spray drying of the supernatant. The study found that preparation of these hydrolysates affected the protein, ash and fat content as well as amino acid composition. As for essential amino acids, their values were generally considered as adequate as compared to the suggested essential amino acids profile of FAO/WHO. The results showed that SFHs were rich in lysine and glutamate. Hydrolysate at DH 68% exhibited better peptide solubility and water holding capacity. As degree of hydrolysis increased, emulsifying capacity and foaming capacity of the hydrolysate decreased. It was also found that the lightness in hydrolysate powder decreased with increase in degree of hydrolysis. This study shows that silver catfish frame hydrolysate has good solubility, good foaming properties and light colour profile, thus having high potential as food ingredient.
    Matched MeSH terms: Amino Acids, Essential
  13. Fulltext Role of amino acid arginine for broiler production: A review
    M. J. Khatun, T.C. Loh, H.L. Foo, M.K.I. Khan
    Journal of Engineering and Science Research, 2018;2(3):1-6.
    MyJurnal
    Amino acids are known as anabolic factors that are essential for formation of muscle by stimulating protein synthesis while inhibiting proteolysis, and they are significant component for the synthesis of various nitrogenous compounds. There are 20 amino acids are essential to require in cell for formation of body protein of which about 10 amino acids, which cannot be synthesized by the birds are termed essential. Among the essential amino acid arginine one of the essential amino acids for chickens because, like other birds, they are unable to obtain Arginine from endogenous sources due to the absence of most of the enzymes involved in the urea cycle. This amino acid involved in synthesis of proline, hydroxyl proline and polyamines which are essential for connective tissue synthesis as well as increased growth of chicken. Moreover, L-arginine (L-Arg) is effective for reducing fat deposition in broiler. Moreover, it decrease heat stress increase meat quality and increase immune response of broiler. This re-view presents the recent advances in the relevance of the inclusion of excess L-Arginine in broiler ration to growth, fat deposition and immune response in broiler.
    Matched MeSH terms: Amino Acids, Essential
  14. Fulltext Biochemical properties of red tilapia (Oreochromis niloticus) protein hydrolysates
    Shamloo, M., Bakar, J., Mat Hashim, D., Khatib, A.
    International Food Research Journal, 2012;19(1):183-188.
    MyJurnal
    The amino-acid composition, 2, 2-Diphenyl-1-picryhydrazyl (DPPH) radical-scavenging activity, and peptide patterns of tilapia protein hydrolysates produced by the enzymatic hydrolysis of Alcalase (AH), Flavourzyme (FH) and Protamex (PH) for 5h using pH-stat method were studied. The ratio of essential amino acids to non-essential amino acids increased after hydrolysis in all samples; however, no significant differences among them were observed. AH had a highest (P < 0.05) DPPH radical-scavenging activity, but no significant difference in the DPPH between FH and PH was observed. SDS-PAGE patterns for all the hydrolysates showed significant (P < 0.05) reduction in the number and the intensity of the bands with increasing time of hydrolysis. Flavourzyme showed the lowest rate of hydrolytic activity towards the tilapia mince.
    Matched MeSH terms: Amino Acids, Essential
  15. Fulltext Effects of Seasonal Variability on the Physicochemical, Biochemical, and Nutritional Composition of Western Peninsular Malaysia Gracilaria manilaensis
    Aroyehun AQ, Palaniveloo K, Ghazali F, Rizman-Idid M, Abdul Razak S
    Molecules, 2019 Sep 10;24(18).
    PMID: 31510066 DOI: 10.3390/molecules24183298
    This study evaluated the effect of seasonal variation on the physicochemical, biochemical, and nutritional composition of Gracilaria manilaensis. Sampling was designed during the main monsoon seasons in Malaysia-the Southwest monsoon (SWM) and Northeast monsoon (NEM)-to understand the intraspecific variation (p < 0.05). Carbohydrates, protein, and dietary fiber were found to be higher in NEM-G. manilaensis, whereas a higher ash content was quantified in SWM-G. manilaensis. No significant differences were found in crude lipid and moisture content (p > 0.05). Vitamin B2 was calculated as (0.29 ± 0.06 mg 100 g-1) and (0.38 ± 0.06 mg 100 g-1) for the NEM and SWM samples, respectively (p < 0.05). The fatty acid profile showed the dominance of saturated fatty acids (SFAs)-palmitic acids, stearic acid, and myristic acid-while the mineral contents were found to be good sources of calcium (1750.97-4047.74 mg 100 g-1) and iron (1512.55-1346.05 mg 100 g-1). Tryptophan and lysine were recorded as the limiting essential amino acids (EAAs) in NEM G. manilaensis, while leucine and phenylalanine were found to be the limiting EAAs in the SWM samples. None of the extracts exhibited antibacterial properties against the screened strains. The study concluded that seasonal changes have a great effect on the biochemical composition of G. manilaensis.
    Matched MeSH terms: Amino Acids, Essential
  16. Fulltext Urinary amino acids profile of vegetarians and non-vegetarians
    Chia, Yoke Yin, Ton, So Ha
    Malays J Nutr, 2006;12(1):67-78.
    MyJurnal
    The objective of the study was to quantify and to profile the amino acids content in urine samples. The amino acids content in urine was determined in 162 individuals (62 young non-vegetarians aged 15-45 years, 24 elderly non-vegetarians aged 46-70 years, 40 young vegetarians and 36 elderly vegetarians) by high performance liquid chromatography (HPLC). The most common amino acids detected in the young and elderly individuals on vegetarian and non-vegetarian diets were phenylalanine, threonine, arginine and asparagine, while leucine, aspartic acid and alanine were not found in any urine samples in both groups. Isoleucine was not detected in the urine of vegetarians. The concentrations of the majority of essential amino acids were between 0.10 - 2.00 mgl24hrs except for histidine which had a range of 4.1 - 5.0 mgl24hrs. The concentrations of non-essential amino acids varied. Proline, glycine and tyrosine concentrations were between 0.10 - 1.00 mg/24hrs, while cysteine, glutamine, glutamic acid and cystine concentrations were between 11.0 - 21.0 mg124hrs. Asparagine and hydroxy-proline had a range of 0.10 - 5.00 mg/24hrs, while serine and arginine ranged between 31.0 - 50.0 mg124hrs. Isoleucine and serine were not detected in elderly vegetarians while histidine, glycine, glutamic acid and hydroxy-proline were not detected in elderly non-vegetarians. Isoleucine, glycine and hydroxy proline were detected in young non-vegetarians but not in young vegetarians. The levels of amino acids showed no significant statistical differences between young vegetarians and non-vegetarians as well as between elderly vegetarians and non-vegetarians. Phenylalanine, threonine and trypthophan were commonly detected in the lacto-ovo and lacto vegetarians, while valine, cysteine, arginine and asparagine were commonly detected in vegans. In conclusion, except for isoleucine, general differences were seen in urinary amino acid excretions between vegetarians and non-vegetarians even though the differences were statistically not significant. Therefore lacto-ovo diets could be nutritionally adequate as the nutrients were substituted by dairy or plant products.
    Matched MeSH terms: Amino Acids, Essential
  17. Fulltext Composition of tree peony (Paeonia suffruticosa) and Chinese apple flower (Malus spp.) buds
    Voon, H.C., Bhat, Rajeev, Karim, A.A., Rosma, A.
    International Food Research Journal, 2013;20(3):1173-1179.
    MyJurnal
    Traditionally, in Chinese medicine, tree peony and apple flower buds are used to prepare herbal decoctions to cure various ailments. As both of these flowers are popular and used regularly, providing scientific evidence on their basic composition is a necessity. Hence, in the present study, we report the chemical composition of these two flower buds. Results revealed tree peony and apple flower buds to have high crude protein (15.73 and 26.30%), fibre (13.11 and 16.51%), and carbohydrate (57.84 and 40.63%) contents. Both the flowers had significant amounts of essential amino acids and unsaturated fatty acids. Essential minerals present in tree peony and apple flowers were potassium (1540.37 and 1125.60 mg/100 g), calcium (462.46 and 449.98 mg/100 g), magnesium (241.51 and 164.23 mg/100 g), sodium (12.75 and 20.06 mg/100 g), and phosphorus (420.00 and 590.00 mg/100 g), respectively. Heavy metals (cadmium, nickel, mercury, lead, and arsenic) were detected in trace amounts (< 0.50 mg/100 g) in both the flower buds. Results obtained indicate that both flowers could be exploited as an additional source of nutraceutical for the development of new functional foods.
    Matched MeSH terms: Amino Acids, Essential
  18. Fulltext Ficus carica L
    Muhammad Aiman, Mohammad Rahimi, Siti Zaharah Sakimin, Mohd Fauzi, Ramlan
    The Pertanika Journal of Scholarly Research Reviews, 2019;5(1):37-43.
    MyJurnal
    Ficus carica L. or fig is the oldest fruit tree that being cultivated by man. Grouped under genus Ficus, this species is grown widely in Mediterranean region and now being cultivated in an area with temperate or sub-temperate climate. Fig planting in Malaysia is still new, which was brought by a man as a hobby at first. Fig is a unique fruit tree as some variety can produce fruits without pollination. Contain lots of carbohydrates, essential amino acids, vitamins and minerals such potassium, fibre, calcium, iron compared to other fruits, fig have become an important source of diet to people especially in Mediterranean region since ancient time.
    Matched MeSH terms: Amino Acids, Essential
  19. Fulltext Chemical composition and molecular structure of polysaccharide-protein biopolymer from Durio zibethinus seed: extraction and purification process
    Amid BT, Mirhosseini H, Kostadinović S
    Chem Cent J, 2012 Oct 14;6(1):117.
    PMID: 23062269 DOI: 10.1186/1752-153X-6-117
    BACKGROUND: The biological functions of natural biopolymers from plant sources depend on their chemical composition and molecular structure. In addition, the extraction and further processing conditions significantly influence the chemical and molecular structure of the plant biopolymer. The main objective of the present study was to characterize the chemical and molecular structure of a natural biopolymer from Durio zibethinus seed. A size-exclusion chromatography coupled to multi angle laser light-scattering (SEC-MALS) was applied to analyze the molecular weight (Mw), number average molecular weight (Mn), and polydispersity index (Mw/Mn).

    RESULTS: The most abundant monosaccharide in the carbohydrate composition of durian seed gum were galactose (48.6-59.9%), glucose (37.1-45.1%), arabinose (0.58-3.41%), and xylose (0.3-3.21%). The predominant fatty acid of the lipid fraction from the durian seed gum were palmitic acid (C16:0), palmitoleic acid (C16:1), stearic acid (C18:0), oleic acid (C18:1), linoleic acid (C18:2), and linolenic acid (C18:2). The most abundant amino acids of durian seed gum were: leucine (30.9-37.3%), lysine (6.04-8.36%), aspartic acid (6.10-7.19%), glycine (6.07-7.42%), alanine (5.24-6.14%), glutamic acid (5.57-7.09%), valine (4.5-5.50%), proline (3.87-4.81%), serine (4.39-5.18%), threonine (3.44-6.50%), isoleucine (3.30-4.07%), and phenylalanine (3.11-9.04%).

    CONCLUSION: The presence of essential amino acids in the chemical structure of durian seed gum reinforces its nutritional value.

    Matched MeSH terms: Amino Acids, Essential
  20. Optimization of hexametaphosphate-assisted extraction and functional characterization of palm kernel cake protein
    Chee KL, Ayob MK
    Food Sci Technol Int, 2013 Apr;19(2):109-22.
    PMID: 23520324 DOI: 10.1177/1082013212442185
    Response surface methodology was applied to study the optimization of palm kernel cake protein (PKCP) hexametaphosphate-assisted extraction. The optimum PKCP yield (28.37%) when extracted using 1.50% sodium hexametaphosphate (SHMP) of pH 10, at 50 °C, and the 1:70 (w/v) ratio of cake-to-solvent was significantly (P amino acid profile showed deficiencies. Therefore, it can be used as a complementary protein source by supplementing with a tryptophan-rich source, as this was the limiting amino acid.
    Matched MeSH terms: Amino Acids, Essential
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