Soil is an important sink for microplastics (MPs) and pesticides. MPs can act as carriers for pesticides, thus induce direct and indirect effects on soil organisms. Fluindapyr (FIP), a novel succinate dehydrogenase inhibitors fungicides (SDHIs), may pose a serious threat to earthworms. However, few studies have evaluated the effects of joint exposure to MPs and FIP. Here, earthworms (Eisenia fetida) were jointly exposed to PMMA (polymethylmethacrylate) and PS (polystyrene) MPs of different sizes (0.1, 1 and 10 μm) along with FIP for 28-day to investigate the toxic effects of single and joint exposure of FIP and MPs on earthworms. The results showed that joint exposure to 0.1 and 1 μm MP promoted the accumulation of FIP in earthworms at the beginning of the experiment compared to the sole group, but the elimination of FIP from earthworms accelerated after 14 d. In addition, the joint exposure caused more serious damages to the epidermis and intestine of earthworms and increased the severity of oxidative stress. The effects of joint exposure to FIP and MPs depended on the size of the MPs, and the strongest effects were observed in the treatment with the smallest size. The 16S rRNA sequencing results showed that the joint exposure to MPs and FIP didn't cause gut microbiota dysbiosis. However, the sole 0.1 μm PS significantly altered the community diversity and richness of earthworm gut bacteria, and the relative abundance of Proteobacteria, Actinobacteria and Firmicutes was significantly changed. The obtained results inferred that MPs could influence environmental and toxicological behaviors of FIP and may provide data support for the risk assessments of MPs and FIP on soil ecosystems.
The mechanical properties of tissue scaffolds are essential in providing stability for tissue repair and growth. Thus, the ability of scaffolds to withstand specific loads is crucial for scaffold design. Most research on scaffold pores focuses on grids with pore size and gradient structure, and many research models are based on scaffolding with vertically arranged holes. However, little attention is paid to the influence of the distribution of holes on the mechanical properties of the scaffold. To address this gap, this research investigates the effect of pore distribution on the mechanical properties of tissue scaffolds. The study involves four types of scaffold designs with regular and staggered pore arrangements and porosity ranging from 30% to 80%. Finite element analysis (FEA) was used to compare the mechanical properties of different scaffold designs, with von-Mises stress distribution maps generated for each scaffold. The results show that scaffolds with regular vertical holes exhibit a more uniform stress distribution and better mechanical performance than those with irregular holes. In contrast, the scaffold with a staggered arrangement of holes had a higher probability of stress concentration. The study emphasized the importance of balancing porosity and strength in scaffold design.
Cardiogenic shock (CS) remains a major clinical challenge, with persistently high short-term mortality despite advances in reperfusion therapy, pharmacological support, and mechanical circulatory support. This review reassesses the conceptual evolution of CS and examines why hemodynamic-centric diagnostic and therapeutic strategies have failed to yield meaningful improvements in outcomes. Historically, CS has been defined by cardiac-oriented macrocirculatory compromise, and contemporary definitions and staging systems have improved risk stratification but remain largely hemodynamics-focused and primarily validated in intensive care settings. Increasing evidence indicates that inflammatory activation, microcirculatory dysfunction, endothelial injury, and metabolic derangements are central determinants of shock progression and organ failure. Integrating multimodal diagnostic approaches-including bedside assessment of microcirculation and inflammation, omics-based phenotyping, advanced echocardiographic techniques, and artificial intelligence-assisted risk stratification-may enable earlier recognition, more precise classification, and improved risk stratification across the continuum of care. Reframing cardiogenic shock as a dynamic, multisystem disorder supports a shift from hemodynamic-centric paradigms toward multimodal precision care, with important implications for clinical management and future trial design.