The objective of the present study is to evaluate the effect of incorporation of 3- acetylcoumarin (3-AC), an antibacterial agent, on the mechanical and surface morphology of glass ionomer cement (GIC). A conventional GIC, Fuji II LC, was used as a control. 3-AC was incorporated into GIC during its manipulation at percentage of 2% and 5% (wt/wt). Flexural strength of the specimens were analysed using Shimadzu AGX-Plus while morphological evaluation of the specimens were observed using Scanning Electron Microscope (SEM). Oneway analysis (ANOVA) with post-hoc Bonferroni multiple-range test was used to determine the significant differences among the groups. Statistically, the incorporation of 2% (wt/wt) of 3-AC into GIC showed a significantly lower flexural strength (p