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  1. Chang YJ, Pong LY, Hassan SS, Choo WS
    Access Microbiol, 2020;2(1):acmi000073.
    PMID: 33062932 DOI: 10.1099/acmi.0.000073
    This study investigated the antiviral activity of betacyanins from red pitahaya (Hylocereus polyrhizus) and red spinach (Amaranthus dubius) against dengue virus type 2 (DENV-2). The pulp of red pitahaya and the leaves of red spinach were extracted using methanol followed by sub-fractionation and Amberlite XAD16N column chromatography to obtain betacyanin fractions. The half maximum cytotoxicity concentration for betacyanin fractions from red pitahaya and red spinach on Vero cells were 4.346 and 2.287 mg ml-1, respectively. The half-maximal inhibitory concentration (IC50) of betacyanin fraction from red pitahaya was 125.8 μg ml-1 with selectivity index (SI) of 5.8. For betacyanin fraction from red spinach, the IC50 value was 14.62 µg ml-1 with SI of 28.51. Using the maximum non-toxic betacyanin concentration, direct virucidal effect against DENV-2 was obtained from betacyanin fraction from red pitahaya (IC50 of 126.70 μg ml-1; 95.0 % virus inhibition) and red spinach (IC50 value of 106.80 μg ml-1; 65.9 % of virus inhibition). Betacyanin fractions from red pitahaya and red spinach inhibited DENV-2 in vitro.
  2. Tiew WT, Janapatla RP, Chang YJ, Chen YC, Yang HP, Su LH, et al.
    Infection, 2022 Jan 11.
    PMID: 35018587 DOI: 10.1007/s15010-021-01736-0
    PURPOSE: Antimicrobial resistance of nontyphoidal Salmonella (NTS) is a threat to public health worldwide.

    METHODS: A study on longitudinally collected NTS isolates from a medical center in Taiwan from 2011 to 2019 was undertaken. The multidrug resistance (MDR) and extensively drug resistance (XDR) phenotypes were determined according to internationally used definitions. Molecular serotyping was performed on the resistant NTS.

    RESULTS: Notably 16.1% (870/5412) of the isolates were MDR, while XDR accounted for 2.1% (111/5412). Both MDR and XDR NTS have increased significantly from 2011 to 2019, especially from 2015 to 2017 (MDR from 9.6% in 2015 to 23.1% 2017; XDR from 1.4% in 2016 to 4.7% in 2017). S. Anatum was the commonest NTS serotype expressing MDR and XDR, in 256/559 (45.8%) and 81/111 (73.0%) of the isolates, respectively, followed by S. Typhimurium and S. Goldcoast. Children 

  3. O'Brien E, Nishimata N, Obama K, Okamura M, Shimazu T, Kobayashi N, et al.
    Asian Pac J Cancer Prev, 2026 Oct 01;27(10):3599-3609.
    PMID: 42935050 DOI: 10.31557/APJCP.2026.27.10.3599
    OBJECTIVES: Effective communication between cancer patients and healthcare providers is essential for patient-centered care and often depends on patients asking questions during consultations. The Question Prompt List (QPL) is a structured communication tool designed to encourage patients to ask questions and support informed decision-making. Most studies on QPL implementation have been conducted in Western countries, where communication practices differ from those in Asia. This study aimed to assess awareness of and willingness to use QPLs among cancer care providers affiliated with the Asian National Cancer Centers Alliance (ANCCA).

    STUDY DESIGN: Cross-sectional, semi-structured online survey.

    METHODS: An anonymized, self-administered, semi-structured online survey was conducted via Google Forms between July and October 2024. Survey invitations were distributed via email, professional networks, and social media where appropriate. The survey assessed respondents' demographics, respondents' prior use of QPLs (Yes/No), prior awareness of the existence of QPLs (Yes/No), preferred communication style (single-response, multiple-choice), and perceived barriers and facilitators to implementation (open-ended response). All questions excluding the two open-ended questions were required for submission. Descriptive statistics were used to analyze quantitative data, and thematic analysis following Braun and Clarke's framework was used for open-ended responses.

    RESULTS: Among 85 respondents, 84.7% reported being unaware of QPLs, and 22.4% reported having implemented QPLs in their clinical practice. Among respondents who had not implemented QPLs, 68.2% indicated that they were "Somewhat" or "Very" willing to implement them.  Most respondents favored communication styles that emphasize sharing information (16.4%), open and inclusive communication (15.6%), and supportive counseling (15.6%). QPLs are aligned with current attitudes toward patient-provider communication in ANCCA countries.

    CONCLUSION: There is substantial opportunity to introduce QPLs across ANCCA countries. This study provides foundational evidence to guide their effective implementation, promoting patient-centered care in Asia.

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