This study aimed to analyze the estimated suicide trends among males in the Association of Southeast Asian Nations (ASEAN) countries from year 2000 to 2019 and their relationship with unemployment rates. Age-standardized suicide rate and unemployment data of 10 ASEAN countries (Brunei, Cambodia, Indonesia, Lao People's Democratic Republic, Malaysia, Myanmar, the Philippines, Singapore, Thailand, Vietnam) were analyzed using Joinpoint regression. Results showed that ASEAN countries (except the Philippines and Myanmar) recorded an increase or a plateau in the decline of age-standardized suicide rates in males during the second half of the 2010s. This was in contrast with global trends for males, which has been steadily decreasing. Unemployment was significantly associated with suicide rates. Our study found generally increasing or plateauing suicide trends in ASEAN countries between years 2013 and 2017. However, limitations in data quality highlight the importance of improved suicide surveillance to effectively support prevention efforts.