MATERIALS AND METHODS: Ninety-eight female rats (Sprague Dawley) were divided into donor and recipient groups, with donors further divided into seven subgroups, including negative control, Cd-exposed, EBN-treated, and EDTA-treated groups. Embryos from donors were transferred to recipient rats, with EBN and Cd administered for 4 weeks and EDTA given only in the last 5 days for the donor group.
RESULTS: Results showed significant differences in pregnancy rates and blastocyst quality. EBN at 120 mg/kg BW led to higher blastocyst production and better quality compared to Cd-exposed groups. The highest pregnancy rates in recipient groups correlated with the highest blastocyst scores from donors.
CONCLUSION: EBN at 120 mg/kg demonstrated significant protection against Cd toxicity and its effect on pregnancy rates, embryo production, quality, and pre- and post-embryo transfer, surpassing the effects of both 90 mg/kg EBN and EDTA. This study provides empirical evidence in support of the conventional belief in the positive impact of EBN on female reproduction.