Kappaphycus striatus is one of the carrageenan-producing red algae, and found primarily in tropical and subtropical coastal regions. Its global distribution is mainly in the Philippines, Indonesia, and Malaysia, among other locations. Here, through the high-quality chromosome-level genome sequences and assembly with PacBio HiFi and Hi-C sequencing data, we assembled one genome with a total of 211.46 Mb in size, containing a contig N50 length of 5.04 Mb and a scaffold N50 length of 5.39 Mb. After Hi-C assembly and manual adjustment to the heatmap, we deduced that 199.42 Mb of genomic sequences were anchored to 33 presumed chromosomes, which accounting for 94.31% of the entire genome. One total of 14,596 protein-coding genes and 1,673 non-coding RNAs were identified, and the 100.96 Mb of repetitive sequences accounting for 47.73% of the assembled genome. Our chromosome-level genome assembly data provide valuable references for K. striatus future nursery and breeding, and will be useful for the functional genomics interpretations and evolutionary studies of eukaryotes.