A novel bacteriophage, PA-1, was isolated using Pantoea ananatis, a bacterium that is responsible for rice leaf blight worldwide, as the host. PA-1 has a linear genome of 46,332 bp with a GC content of 50.55%. It contains 83 protein-encoding genes, and no tRNA-encoding genes were detected. The genome is densely organized, with an average CDS size of 516 bp, and 92.38% of the genome consists of protein-coding regions. Whole-genome sequencing and phylogenetic and morphological analysis showed that PA-1 is a novel phage that is phylogenetically distinct, suggesting that it may represent a new genus within the class Caudoviricetes..