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  1. Leninkumar R, Garibaldi A, Venkatesh L
    Int J Lang Commun Disord, 2025;60(5):e70120.
    PMID: 40939100 DOI: 10.1111/1460-6984.70120
    BACKGROUND: Tamil is a member of the Dravidian language family, and it is spoken extensively in several South Asian countries, including India, Sri Lanka, Malaysia and Singapore. The phonological development of Tamil-speaking children is examined in the current study within the context of lexical development by including children with typical language development (TD) and late talkers (LTs). The current study was conducted amongst Tamil-speaking children in Tamil Nadu, a southern state of India.

    AIMS: The study aimed to compare the size and nature of the phonetic inventory, the proportion of consonants, vowels and syllable structure types amongst Tamil-speaking children with TD and LTs around 24 months.

    METHODS AND PROCEDURES: The study included 68 Tamil-speaking children, comprising 34 LTs and 34 age- and gender-matched TD children. All children were born preterm and were assessed after their second birthdays as per their adjusted age for prematurity (M = 24.38 months; SD = 1.45). Language samples were recorded during free play and interaction with the caregiver. The transcription of children's spontaneous speech was subjected to independent analyses of speech to derive the phonological characteristics.

    OUTCOMES AND RESULTS: LTs produced a significantly lower phonetic inventory (Mdn = 8.5) than TD children (Mdn = 15.5). Only consonants /p/ and /m/ were produced by 90% of Tamil-speaking LTs. In comparison, 90% of TD children produced a wide range of consonants over different manners of articulation, including nasals, semi-vowels and stops. Tamil's retroflex consonants and geminate clusters emerged early in TD children's productions; fricatives and affricates were limited. LTs produced a significantly higher proportion of front vowels and a significantly lower proportion of mid- and back vowels than TD children. Syllable structures produced by LTs predominantly consisted of monosyllables and reduplicated syllables compared to TD children's wide range of complex structures.

    CONCLUSIONS AND IMPLICATIONS: The data contribute to an emerging database on early phonological development in Tamil as spoken in Tamil Nadu, India. Observations from 2-year-old LTs and TD children speaking Tamil revealed similarities with English (bilabials, alveolar stops, disyllable and monosyllable production) and differences in line with distinct phonological characteristics of Tamil (lesser production of fricatives, back vowels and closed syllables). The study provides clinical information relevant to the assessment and intervention of young children with phonological and lexical delays.

    WHAT THIS PAPER ADDS: What is already known on this subject Research from English and other Indo-European languages has revealed bidirectional relationships between phonological and lexical development, as evidenced by findings from typically developing (TD) children and children with language delays. Children identified as Late talkers (LTs) at 2 years of age demonstrate limited phonetic inventory and syllable shapes compared to TD children. What this paper adds to existing knowledge The current study is the first to report on the size and nature of the phonetic inventory, the proportion of consonants, vowels and syllable structure types amongst Tamil-speaking children born preterm who were TD and late-talking. There are considerable differences in TD children's and late talkers' early phonological abilities. The characteristics of Tamil influenced the phonological skills of both groups of children as the ambient language of exposure. What are the potential or actual clinical implications of this work? The study contributes to the much-needed data corpus on early phonological skills, specifically the phonetic inventory and syllable structures from Tamil-speaking children. The data will aid in the identification of delays and atypical patterns in phonological development amongst children learning to speak Tamil. The information will guide intervention programs for children with early language delays. The findings strengthen existing cross-linguistic research on the relationship between early phonological skills and lexical development amongst children.

  2. Srinivasan S, Yeri A, Cheah PS, Chung A, Danielson K, De Hoff P, et al.
    Cell, 2019 04 04;177(2):446-462.e16.
    PMID: 30951671 DOI: 10.1016/j.cell.2019.03.024
    Poor reproducibility within and across studies arising from lack of knowledge regarding the performance of extracellular RNA (exRNA) isolation methods has hindered progress in the exRNA field. A systematic comparison of 10 exRNA isolation methods across 5 biofluids revealed marked differences in the complexity and reproducibility of the resulting small RNA-seq profiles. The relative efficiency with which each method accessed different exRNA carrier subclasses was determined by estimating the proportions of extracellular vesicle (EV)-, ribonucleoprotein (RNP)-, and high-density lipoprotein (HDL)-specific miRNA signatures in each profile. An interactive web-based application (miRDaR) was developed to help investigators select the optimal exRNA isolation method for their studies. miRDar provides comparative statistics for all expressed miRNAs or a selected subset of miRNAs in the desired biofluid for each exRNA isolation method and returns a ranked list of exRNA isolation methods prioritized by complexity, expression level, and reproducibility. These results will improve reproducibility and stimulate further progress in exRNA biomarker development.
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