Ultrasmall metal nanoclusters (NCs, <2 nm) have emerged as a novel class of luminescent probes due to their atomically precise size and tailored physicochemical properties. The rapid advancements in the design and utilization of metal NC-based luminescent probes are facilitated by the atomic-level manipulation of metal NCs. This review article explores (i) the engineering of metal NCs' functions for bioimaging applications, and (ii) the diverse uses of metal NCs in bioimaging. We begin by presenting an overview of the engineering functions of metal NCs as luminescent probes for bioimaging applications, highlighting key strategies for enhancing NCs' luminescence, biocompatibility and targeting capabilities towards biological specimens. Our discussion then centers on the bioimaging applications of metal NCs in subcellular organelles, individual cells, tissues, and entire organs. Finally, we offer a perspective on the challenges and potential developments in the future use of metal NCs for bioimaging applications.
Being abundant in many tropical part of the world, Dioscorea sp. as food is limited due to its toxicity. However polysaccharides derive from these tubers could be important for other applications. Here we developed a Highly Luminescent Carbon Nanodots (C-dots) via acid hydrolysis of Gadong starch (GS). The hydrolysis rate of GS increased from 49% to 86% within 7 days while the X-ray diffraction showed the native GS particle is a C-crystalline type. The GS particles were either round or oval with diameters ranging from 50-90 nm. Further acid dehydration and surface oxidation reduced the size of GS nanoparticles to 6-25 nm. The C-dots produced a fluorescent emission at wavelength 441 nm. Toxicity tests demonstrate that zebrafish embryo were able to tolerate the C-dots for 48 h after exposure. This study has successfully demonstrated a novel approach of converting GS into excellent fluorescent C-dot.