The objective of the present study was to determine the effects of dietary supplementation of graded levels of coffee by-products (CBP) on growth performance, gut health, and toxicity in broiler chickens. A total of 60 (14 days old) Cobb 500 broiler chickens were used in this experimental study. The three treatment diets were prepared with 0%, 1.5% and 2.5% CBP in a complete randomized experiment. There were no differences in total feed intake (TFI) across treatments (P > 0.05). However, chickens fed diets with 2.5% CBP had lower total weight gain (TWG) and higher feed conversion ratio (FCR) compared to those on the control and 1.5% CBP diet (P