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  1. Zailani NIM, Hanis F, Anuar MAM
    J Equine Vet Sci, 2024 Mar;134:105018.
    PMID: 38316315 DOI: 10.1016/j.jevs.2024.105018
    This study aimed to evaluate the benefits of using sawdust, pinewood shavings, and rubber wood shavings as horse bedding materials. Nine horses were used in a repeated measure design, where each horse was provided with each of the bedding materials for two consecutive weeks. The weight, cost, mucking out time, ammonia emissions, and urine holding capacity were measured and calculated for each bedding material. Results showed that pinewood shavings had the highest urine-holding capacity, while rubber wood shavings had the lowest absorbency for urine. Pinewood shavings also had lower ammonia emissions compared to sawdust and rubber wood shavings but were significantly more expensive. Sawdust was the cheapest of the three bedding materials, but it was used in greater quantities, which required more time for mucking out. The findings from this study could be used to make informed decisions on bedding materials for stables, taking into consideration the trade-off between cost, maintenance requirements, and labor hours.
  2. Hanis F, Chung ELT, Kamalludin MH, Idrus Z
    J Equine Vet Sci, 2020 11;94:103230.
    PMID: 33077069 DOI: 10.1016/j.jevs.2020.103230
    The present study aimed to provide preliminary data on the prevalence of oral stereotypic, locomotory stereotypic, and redirected behaviors as well as their associations with stable management and feeding practices. In this cross-sectional study, a total of 207 working horses used for leisure riding, equestrian sport, polo, endurance, and patrolling were selected from seven equine facilities. Data on the stable management and feeding practices were obtained through the stable records, interviews, and on-site monitoring visits, whereas the prevalence of abnormal behaviors in the studied population was determined using the instantaneous scan sampling method. Most horses in the present study worked for more than 8 hours per week (n = 93). In addition, more horses were fed three times per day (n = 65) with different amounts of hay, concentrate, and chaff. Among the study population, oral stereotypic behaviors had the highest prevalence (n = 281; 54%), followed by redirected behavior (n = 181; 34%), and locomotory stereotypic behaviors had the lowest prevalence (n = 63; 12%). The oral stereotypic behavior was found to be significantly influenced (P < .05) by the working hours, amount of hay, and amount of concentrate. Both locomotory stereotypic and redirected behaviors were found to be influenced (P < .05) by the number of feedings per day and the amount of hay. In summary, the present study has proven that the stable management and feeding practices could influence the prevalence of different abnormal behaviors in the majority of working horses, with oral stereotypy being the most common abnormal behavior in this country.
  3. Hanis F, Chung ELT, Kamalludin MH, Idrus Z
    J Equine Vet Sci, 2022 Nov;118:104130.
    PMID: 36182046 DOI: 10.1016/j.jevs.2022.104130
    The high prevalence of abnormal oral behavior (AOB) in working horses has been linked to management issues and the pathophysiology of this behavior remains unclear. Therefore, this study aims to elucidate the blood profile, hormones, and telomere length responses between low and high levels of AOB among different horse working groups. A total of 207 healthy horses from various breeds were initially selected from four working groups (leisure riding, equestrian, endurance, and patrolling) and observed for the time spent on AOB. Then, six horses each with higher and lower AOB than the population means were randomly selected from each of the working groups and categorized as high and low AOB horses, respectively. Blood samples were collected for hematology, biochemistry, cortisol, ghrelin, leptin, and relative telomere length analyzes. High AOB horses notably had higher values of glucose, alanine aminotransferase (ALT), alkaline phosphatase (ALP), and creatine kinase (CK) compared to low AOB horses. High AOB horses also recorded higher plasma cortisol and ghrelin, but lower leptin concentrations. Among working groups, both endurance and patrolling horses presented the highest values in sodium, potassium, chloride, phosphate, ALT, and CK. While patrolling horses had the lowest levels of urea, ALP, and albumin levels, equestrian and leisure horses recorded the highest and lowest plasma cortisol and leptin concentrations, respectively. Finally, the telomere length of endurance and patrolling horses were significantly greater than leisure and equestrian horses. The present findings suggest that AOB horses had distinctive physiological characteristics that could be linked to improper diet and a demanding workload, while ghrelin and leptin hormones could be potential biomarkers for this behavior.
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