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Fulltext Evaluation of image quality of bitewing radiographs taken by UITM dental students.

Ayoub,A,A,, A,Rasid,L,H,, Razak,S,, Kamaruzaman,M,, Azmi,N,W,

Compendium of Oral Science, 2020;7(1):32-40.
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Abstract
Objectives: To evaluate the quality of bitewing radiograph taken by Universiti Teknologi MARA (UiTM) dental
students during daily clinical practices and to evaluate the difference in caries lesions found on bitewing
radiographs and clinical examination.
Materials and method: 120 patients who attended the undergraduate dental clinic for dental examination were
included in this study. The inclusion criteria were patients within the age range of 17-45 years old, possessing
at least three sets of posterior teeth, with bitewing radiographs taken by undergraduate dental students. The
number of caries lesions detected by clinical examination, bitewing radiographs, and a combination of both
methods were recorded. The quality of 240 bitewing radiographs was classified into three categories; excellent,
acceptable, and unacceptable. The frequency of radiographic errors; foreshortening/elongation, horizontal
overlapping, inadequate film coverage, non-ideal centering and inadequate contrast and density were also
evaluated.
Results: The quality of bitewing radiographs are mostly accepted to be used as a diagnostic tool and one of the
factors which commonly affected the quality of the bitewing is the overlapping of adjacent teeth. The highest
number of caries lesions were detected radiographically (74%) compared with 25% caries by clinical
examination. The majority of radiographs (71%, n=171) were deemed to be of acceptable quality, 39(16%) were
excellent, and 30(13%) were diagnostically unacceptable. “Horizontal overlap” was the most common error
detected on the radiographs (n=139, 57.9%), followed by “non-ideal centering” (n=93, 38.8%), “inadequate
contrast” (n=46, 19.2%) and “inadequate film coverage” (n=24,10%). The highest number of caries lesions were
detected radiographically (74%) compared with 25% caries by clinical examination.
Conclusion: The quality of the majority of bitewing radiographs taken by undergraduate dental students in this
institution is acceptable. However, given that more than half of the radiographs possessed horizontal
overlapping error, caries diagnosis may have been underestimated. Further training and periodic audits are
required to reduce the percentage of errors in bitewing radiographs amongst undergraduate dental students.
Fulltext Microcomputed tomography (micro-CT) analysis of apical mandibular premolar in relation to clinical sign presentation: An in vitro study

Nurulaqmar Iwani S, Kamaruzaman M, Jawami AA

Saudi Dent J, 2024 Jan;36(1):129-133.
PMID: 38375388 DOI: 10.1016/j.sdentj.2023.10.009

INTRODUCTION: Variations in anatomic apex and apical foramina of root canals are common in different teeth types. The sophisticated 3D micro-CT aids researchers in investigating the apical morphology, such as the apical foramen (AF) and constriction (AC).
OBJECTIVES: To measure the length between the foramen and the apex and the physiological length to the apex, in lower premolar teeth. Furthermore, a measurement was conducted on the average length from the foramen to the apex of the lower premolar teeth with respect to the presence of clinical and non-clinical signs.
METHODS: A total of 80 lower premolar teeth were selected based on the inclusion criteria. All samples were scanned using micro-CT ZEISS X-Radia (17 µm), and the datasets were analysed. The root canal AF and AC were reconstructed using Drishti software (V3.0) by utilising a 4-digit system code in millimetres (mm).
RESULTS: More than half of the lower premolars (n = 47, 58.8 %) demonstrated a clinical signs presentation, while the rest had a non-clinical sign (n = 33, 41.3 %). There was a significant difference (p = 0.013) between the non-clinical and clinical groups in the mean length from the apical foramen to the apex (AFA) at 0.59 mm and 0.47 mm, respectively. Meanwhile, the non-clinical and clinical mean length from apical constriction to apex (ACA) were 0.75 mm and 0.73 mm, and the mean length from AF to AC (AFC) was 0.48 mm and 0.53 mm, respectively. Nonetheless, no significant differences were detected between the ACA and AFC of non-clinical and clinical groups.
CONCLUSION: The clinical signs presentation observed in lower premolars mainly affected the apical morphology, particularly the position and length of the root AF, and less so the AC position and length. Therefore, the information is useful for clinical purposes.
Purification and characterization of alpha-amylase from Aspergillus flavus

Khoo SL, Amirul AA, Kamaruzaman M, Nazalan N, Azizan MN

Folia Microbiol (Praha), 1994;39(5):392-8.
PMID: 7729774

Aspergillus flavus produced approximately 50 U/mL of amylolytic activity when grown in liquid medium with raw low-grade tapioca starch as substrate. Electrophoretic analysis of the culture filtrate showed the presence of only one amylolytic enzyme, identified as an alpha-amylase as evidenced by (i) rapid loss of color in iodine-stained starch and (ii) production of a mixture of glucose, maltose, maltotriose and maltotetraose as starch digestion products. The enzyme was purified by ammonium sulfate precipitation and ion-exchange chromatography and was found to be homogeneous on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The purified enzyme had a molar mass of 52.5 +/- 2.5 kDa with an isoelectric point at pH 3.5. The enzyme was found to have maximum activity at pH 6.0 and was stable in a pH range from 5.0 to 8.5. The optimum temperature for the enzyme was 55 degrees C and it was stable for 1 h up to 50 degrees C. The Km and V for gelatinized tapioca starch were 0.5 g/L and 108.67 mumol reducing sugars per mg protein per min, respectively.

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