The research, focusing on the analysis of nine trace elements, namely As, Cd, Cr, Cu, Fe, Mn, Ni, Pb, and Zn, completely analyzed their quantities in both water and sediment inside the Rabnabad Channel. Samples were collected during the post-monsoon and analyzed by ICP-OES following acid digestion. The mean concentrations of elements in water and sediments are as follows: Fe > Mn > Pb > Cu > Ni > Zn > Cr > As>Cd, and Zn > Fe > Pb > Mn > As>Cu > Cr > Ni > Cd. To understand the state of ecological and human health risk, several indices were incorporated. Health risk assessment revealed that children posed higher risk than adults. PERI, TRI, and Igeo indices for water sediment indicate a significant ecological risk. Moreover, Mn and Pb exhibit elevated HPI values and contribute substantially to contamination factors. Correlation and PCA implicate both anthropogenic and geogenic sources, such as agricultural practices, coal-based power plants, and the Payra seaport, in the elevated concentrations of Cd, Cr, Mn, and Fe in both water and sediment samples.