A compact four-port multi-input, multi-output (MIMO) antenna with good isolation is proposed for sub-6 GHz and Internet of Things (IoT) applications. Four similar L-shaped antennae are placed orthogonally at 7.6 mm distance from the corner of the FR4 substrate. The wideband characteristics and the required frequency band are achieved through the L-shaped structure and with proper placement of the slots on the substrate. To obtain good isolation between the ports, rectangular slots are etched in the bottom layer and are interconnected. The proposed antenna has total dimensions of 40 mm × 40 mm × 1.6 mm. The interconnected ground plane provides good isolation of less than -17 dB between the ports, and the impedance bandwidth obtained by the proposed four-port antenna is about 54% between the frequency range of 3.2 GHz to 5.6 GHz, thus providing a wideband antenna characteristic covering sub-6 GHz 5G bands (from 3.4 to 3.6 GHz and 4.8 to 5 GHz) and the WLAN band (5.2 GHz). The proposed design antenna is fabricated and tested. Good experimental results are achieved when compared with the simulation results. As the proposed design is compact and low profile, this antenna could be a suitable candidate for 5G and IoT devices.